##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526331_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	207018
Sequences flagged as poor quality	0
Sequence length	56
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.25541740331759	39.0	38.0	40.0	33.0	40.0
2	36.83241070824759	39.0	38.0	40.0	32.0	40.0
3	36.54612642378923	39.0	37.0	40.0	30.0	40.0
4	36.75455757470365	39.0	38.0	40.0	32.0	40.0
5	36.427127109719926	39.0	36.0	39.0	31.0	40.0
6	36.50382575428224	39.0	38.0	40.0	31.0	40.0
7	36.7065182737733	39.0	38.0	40.0	32.0	40.0
8	36.50104338753152	39.0	37.0	40.0	31.0	40.0
9	36.51471852689138	39.0	37.0	40.0	31.0	40.0
10	36.424436522428	39.0	37.0	40.0	31.0	40.0
11	35.34706643866717	38.0	35.0	39.0	29.0	40.0
12	33.233332367233764	38.0	31.0	39.0	18.0	40.0
13	32.69938845897458	38.0	31.0	39.0	16.0	40.0
14	31.71552232173048	37.0	28.0	39.0	12.0	40.0
15	32.43440183945357	37.0	30.0	39.0	16.0	40.0
16	32.228564665874465	36.0	30.0	39.0	15.0	40.0
17	32.29061724101286	36.0	30.0	39.0	16.0	40.0
18	32.6571216029524	36.0	30.0	39.0	19.0	40.0
19	33.324788182670105	36.0	31.0	39.0	23.0	40.0
20	33.39418794500961	37.0	31.0	39.0	23.0	40.0
21	34.91882348394826	38.0	35.0	39.0	26.0	40.0
22	34.65153271696181	38.0	35.0	39.0	25.0	40.0
23	34.96385338472983	38.0	35.0	39.0	27.0	40.0
24	34.50110135350549	38.0	34.0	39.0	24.0	40.0
25	34.758885700760324	38.0	35.0	39.0	26.0	40.0
26	35.09272623636592	38.0	35.0	39.0	27.0	40.0
27	35.07331729608053	38.0	35.0	39.0	27.0	40.0
28	34.73205228530853	38.0	35.0	39.0	27.0	40.0
29	34.68958254837744	38.0	35.0	39.0	26.0	40.0
30	34.45617289317837	38.0	35.0	39.0	25.0	40.0
31	34.25077046440406	38.0	34.0	39.0	24.0	40.0
32	33.067385444743934	38.0	33.0	39.0	16.0	40.0
33	34.397482344530424	38.0	35.0	39.0	25.0	40.0
34	34.17480605551208	38.0	34.0	39.0	24.0	40.0
35	34.24146209508352	38.0	34.0	39.0	25.0	40.0
36	32.38099102493503	36.0	31.0	38.0	19.0	39.0
37	32.21686036962969	36.0	31.0	38.0	18.0	39.0
38	31.408123931252355	35.0	30.0	38.0	10.0	39.0
39	29.678535199837697	35.0	27.0	38.0	4.0	39.0
40	25.58818073790685	31.0	12.0	38.0	4.0	39.0
41	22.184380102213336	26.0	9.0	33.0	4.0	38.0
42	26.08686684249679	30.0	22.0	35.0	4.0	38.0
43	21.892072186959588	26.0	8.0	33.0	4.0	37.0
44	23.4139543421345	28.0	10.0	34.0	4.0	37.0
45	25.835579514824797	30.0	21.0	35.0	4.0	38.0
46	25.62896946159271	31.0	16.0	36.0	4.0	38.0
47	24.835507057357333	30.0	10.0	36.0	4.0	38.0
48	24.822812509057183	30.0	12.0	36.0	4.0	38.0
49	22.422910085113372	27.0	5.0	35.0	4.0	38.0
50	24.866528514428698	30.0	11.0	36.0	4.0	38.0
51	26.213324445217324	33.0	4.0	38.0	4.0	40.0
52	26.051623530321034	32.0	4.0	38.0	4.0	40.0
53	25.850090330309442	32.0	4.0	38.0	4.0	40.0
54	24.76973982938682	31.0	4.0	38.0	4.0	40.0
55	24.948458588142095	31.0	4.0	38.0	4.0	39.0
56	24.403626737771596	31.0	4.0	38.0	4.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	1.636719862207542
1	2	2.286067700000096
1	3	2.8614812913947603
1	4	2.3978085724729112
1	5	2.4178061322181037
1	6	2.605083973957349
1	7	2.495265791839998
1	8	2.6317918274307246
1	9	2.666608188036733
1	10	2.4340983412481663
1	11	-2.799512392873737
1	12	-6.578901386162837
1	13	-21.001814848541606
1	14	-16.62888575897887
1	15	-9.684571231259149
1	16	-19.808438233435513
1	17	-20.302715801108853
1	18	-19.102973637077813
1	19	-5.393431712503112
1	20	-9.40048084225742
1	21	-7.144661919457754
1	22	-2.0130691617878647
1	23	-2.212305071128789
1	24	-2.3418806904174154
1	25	-2.2440519017293923
1	26	3.0047979333344585
1	27	3.07200104502121
1	28	3.014080761734526
1	29	2.835128414064002
1	30	3.3438100588483906
1	31	2.347073889509815
1	32	-10.739826356096469
1	33	2.015621566049383
1	34	2.2425107247835356
1	35	2.1535458365781537
1	36	4.510758257462509
1	37	4.8372213006880465
1	38	5.636692703126016
1	39	7.51380565562571
1	40	10.26756092587394
1	41	0.9701361413355869
1	42	-3.5815353829391547
1	43	-12.54998487050338
1	44	-15.859890524462111
1	45	-12.117066527122063
1	46	-17.59914606390836
1	47	-17.465898406258063
1	48	-14.121374988167906
1	49	-13.346856159732198
1	50	-14.381620144052654
1	51	-15.29946362548175
1	52	-15.352173888774647
1	53	-15.784515110910085
1	54	-18.012512216648066
1	55	-15.365152430812277
1	56	-14.716755671309034
2	1	1.587462886121294
2	2	2.3452862592186534
2	3	2.9032782478258596
2	4	2.320215433904167
2	5	2.5170794875890152
2	6	2.734584554677447
2	7	2.486242295011621
2	8	2.6401303723546334
2	9	2.6000636214921684
2	10	2.5344884660285345
2	11	-1.4042355829627766
2	12	-8.303252915392413
2	13	-9.924847100842154
2	14	-12.401853983166607
2	15	-5.478668732673722
2	16	-0.7946051830170973
2	17	-0.22582250470336263
2	18	-7.8267295559556835
2	19	-0.5500623447435018
2	20	-1.422875791481637
2	21	3.3889900654137435
2	22	3.9182718549190056
2	23	3.4005035294846877
2	24	3.7769882455246915
2	25	3.4324721162580403
2	26	3.5624232348622016
2	27	3.5042503675144303
2	28	3.517222606339786
2	29	3.7321473842537074
2	30	3.835382172371723
2	31	0.1844722634935536
2	32	-7.487972518876777
2	33	0.8763678658200718
2	34	1.052242997930442
2	35	1.144522339749777
2	36	5.105222059243378
2	37	5.508861313791474
2	38	6.188897950453217
2	39	8.103102537605523
2	40	10.95878162928977
2	41	8.518589043447026
2	42	4.163290252130384
2	43	0.15706417800908312
2	44	-7.604641708237686
2	45	3.989592396610032
2	46	-8.523458878465076
2	47	-11.010971219623558
2	48	-5.833516505187472
2	49	-10.260462878216966
2	50	-5.663403401445667
2	51	-4.792763009025034
2	52	-4.809230382416509
2	53	-5.972251493768422
2	54	-13.482195649746133
2	55	-5.839496514912458
2	56	-4.830383236156113
3	1	1.3766985596330485
3	2	2.2024291163615146
3	3	2.8659886044127134
3	4	2.1164197485309018
3	5	2.106152065244885
3	6	2.5751954227922056
3	7	2.5336883621774646
3	8	2.481556719748802
3	9	2.7216441686046267
3	10	2.322389718901249
3	11	1.780359272104313
3	12	2.009075648622712
3	13	-4.3061948656875835
3	14	-6.154318954624831
3	15	-2.0846439717574228
3	16	3.4480369698482
3	17	2.9260790447072687
3	18	2.7120057098164274
3	19	1.4931580570653793
3	20	1.5487307026988049
3	21	3.5730659791212105
3	22	4.147050860034284
3	23	3.6661273711334417
3	24	3.9011634994352846
3	25	3.4782872248650563
3	26	3.152681964197214
3	27	2.9708527842984793
3	28	2.499207927712753
3	29	2.749346583601323
3	30	2.537806369865976
3	31	3.0198195835321044
3	32	1.7681621093388067
3	33	2.8762195968601816
3	34	2.9676555563113425
3	35	2.8697799570675926
3	36	4.869252009573277
3	37	5.001484933036789
3	38	5.923718915684148
3	39	7.992568028005103
3	40	10.16732192137962
3	41	9.937078182745715
3	42	7.930804523017773
3	43	3.0812987395036338
3	44	3.441099265889381
3	45	7.007384671797222
3	46	0.5114584615897826
3	47	-4.9240856091261165
3	48	0.0320035481893548
3	49	-10.388863797484516
3	50	-0.39525898747873
3	51	-1.6605812312802044
3	52	-1.5503527784428996
3	53	-2.8651271702455503
3	54	-4.649887843385397
3	55	-1.9473621832347945
3	56	0.4840210932975175
4	1	1.9012533085591699
4	2	2.665632579564978
4	3	3.2272720662764414
4	4	2.811773395528647
4	5	2.71550338446081
4	6	3.074780943654318
4	7	3.120398235154262
4	8	3.053420703605049
4	9	3.192743668717668
4	10	2.8539613065656795
4	11	3.5273930249407712
4	12	3.9077836459767425
4	13	5.738066482248811
4	14	4.4236324299029555
4	15	6.708803071206063
4	16	7.151546226548948
4	17	6.979618026679521
4	18	6.253457810262727
4	19	5.434109433219852
4	20	5.200635213404091
4	21	3.9684623300301354
4	22	4.5517750848745635
4	23	4.0788229927265505
4	24	4.407112740394197
4	25	3.9247691761825934
4	26	3.5997333009971015
4	27	3.162450999107527
4	28	3.3156983360793717
4	29	3.2804911321539976
4	30	3.6114161900908854
4	31	4.1680105286282725
4	32	4.859029333668314
4	33	3.87166451300142
4	34	3.6200624659716354
4	35	3.5087058281017818
4	36	5.268793151861036
4	37	5.519851801500369
4	38	6.2610128274461445
4	39	8.647520425704116
4	40	11.045515294737402
4	41	6.933511150165142
4	42	9.200869081283493
4	43	9.932320702711284
4	44	12.385500448201586
4	45	9.905431177193822
4	46	9.95962344940661
4	47	9.979746857569019
4	48	9.171695577629933
4	49	6.161514257729127
4	50	9.917383882224101
4	51	8.162180790708124
4	52	7.8022726747628255
4	53	6.190974046397251
4	54	2.742979766116644
4	55	7.544009114712907
4	56	7.3213526849811394
5	1	2.2067374090462764
5	2	2.923810384100392
5	3	3.5853166907268488
5	4	3.0457963462885047
5	5	3.0343511030677064
5	6	3.1304702874231367
5	7	3.14282904536352
5	8	3.214431777620341
5	9	3.1062131011515817
5	10	3.0742466403368596
5	11	4.107036540165261
5	12	6.747073595441286
5	13	6.738039116477921
5	14	7.132250823349768
5	15	7.202308261580612
5	16	7.748475787054669
5	17	7.264924432094293
5	18	6.541108550050733
5	19	5.859610682923389
5	20	5.559218031348735
5	21	4.186011086711943
5	22	4.23765252270865
5	23	4.080246012813021
5	24	4.5880734613910406
5	25	3.955500936896293
5	26	3.4207429155046114
5	27	3.440286549068304
5	28	3.443751900014938
5	29	3.999955492798364
5	30	3.5677586635916967
5	31	3.8779789667725026
5	32	5.195646559508898
5	33	4.003084066756919
5	34	4.003958621993021
5	35	4.05938723579704
5	36	4.684415358271924
5	37	5.090546709642599
5	38	5.846603836878209
5	39	7.964306589570356
5	40	7.160779921741945
5	41	3.7918659200190064
5	42	9.348616878338937
5	43	13.263820529394739
5	44	12.070912667930482
5	45	9.619504480972122
5	46	10.387240985392598
5	47	10.805709676608295
5	48	10.338380488143859
5	49	10.51975956449942
5	50	10.363582776646957
5	51	9.451427867131596
5	52	9.288653642781362
5	53	9.5242526481888
5	54	9.54669421341929
5	55	9.274401539867522
5	56	9.149322327219295
6	1	2.3427573235641503
6	2	3.1234017607384104
6	3	3.7307302457500953
6	4	3.2704217748733484
6	5	2.982545175680549
6	6	3.3178499314041616
6	7	3.387791705642506
6	8	3.50131355865458
6	9	3.3437313013300596
6	10	3.266158715861735
6	11	4.309234652043521
6	12	6.966015871520376
6	13	7.229772712833189
6	14	8.127342646185706
6	15	7.684859574341871
6	16	7.733751255562485
6	17	7.519797383106454
6	18	6.856382609512476
6	19	5.860432626467613
6	20	6.198173458524536
6	21	4.427169322586252
6	22	5.00875298769386
6	23	4.545559995246919
6	24	4.7805961235487615
6	25	4.394700598725237
6	26	3.940422592363376
6	27	4.073183078118134
6	28	3.7231187686448735
6	29	3.8764995724564386
6	30	4.0833990750331495
6	31	4.403811478162297
6	32	5.513856131628575
6	33	4.550991633334341
6	34	4.57302536380881
6	35	4.241107211373574
6	36	4.293264167627992
6	37	4.426510262317439
6	38	5.209433201398433
6	39	6.383145535603887
6	40	4.074616754206367
6	41	0.9730457612664836
6	42	7.649142922207236
6	43	12.73124808094234
6	44	11.038363703579353
6	45	8.41721243268881
6	46	11.114801926635376
6	47	11.13721125004308
6	48	11.118123102394012
6	49	12.462200032302714
6	50	11.122471508975167
6	51	10.673258687666095
6	52	10.595543878092052
6	53	10.802046082034082
6	54	11.795907496026963
6	55	10.865707725579796
6	56	10.369532744766616
7	1	2.02618055800626
7	2	3.0137973256170483
7	3	3.638506691659593
7	4	3.065197521552001
7	5	2.9788991862689045
7	6	3.2940511101075884
7	7	3.3127991974015742
7	8	3.3708071157417834
7	9	3.2881923941279823
7	10	3.183075107830568
7	11	4.308860064090268
7	12	6.626888911176756
7	13	7.075017943079914
7	14	8.04723076925033
7	15	7.590588272773594
7	16	7.74341562475637
7	17	7.187413005921361
7	18	6.557011917273936
7	19	5.583936771907624
7	20	5.86896059267697
7	21	4.3553982707432795
7	22	4.9109106143045835
7	23	4.279178015424719
7	24	4.917870122150298
7	25	4.288842043136384
7	26	3.5503017253171265
7	27	3.551656757071285
7	28	3.368059334022959
7	29	3.7850501668026553
7	30	3.597933087619303
7	31	4.003177175894791
7	32	4.884947930649648
7	33	4.183221180832099
7	34	4.136455590559386
7	35	3.7985440308721223
7	36	2.6813372871964667
7	37	2.526700053547085
7	38	3.477188670382553
7	39	5.104452098384826
7	40	-1.169389838541612
7	41	-8.618403644387298
7	42	6.28514332176772
7	43	10.520979376517204
7	44	9.250979825844858
7	45	6.807033629372459
7	46	9.865251432179274
7	47	10.19562199822009
7	48	9.99753075397727
7	49	11.841208123402506
7	50	9.849871369128998
7	51	10.866571044074046
7	52	11.18192386011183
7	53	11.258069456322367
7	54	11.632786928651079
7	55	11.041913898789268
7	56	10.426045580647148
8	1	2.060185285672965
8	2	2.7448866588190555
8	3	3.6629575928710594
8	4	2.9537526284169715
8	5	2.863416077828049
8	6	3.3195361884095647
8	7	3.0325618291360357
8	8	3.0987692944081857
8	9	3.1809827024112494
8	10	2.835419242569067
8	11	4.1336655403042
8	12	6.233064425802972
8	13	6.925924079197323
8	14	8.059148429055316
8	15	7.375257828569914
8	16	7.843853918856642
8	17	7.485551487158435
8	18	6.772317738213562
8	19	5.762356943285546
8	20	5.775121333344757
8	21	4.302113527317694
8	22	4.555695406976703
8	23	4.1913468325231165
8	24	4.672105323568601
8	25	4.0131353554578695
8	26	3.599203527740052
8	27	3.4395224125425727
8	28	3.0074762551300154
8	29	3.4606140395496254
8	30	3.2940820091203378
8	31	3.943810486246413
8	32	4.91168879541868
8	33	3.7087062591340754
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115	29	2.6347315886671794
115	30	2.4031812432195707
115	31	1.0435024609383845
115	32	-4.011346502616615
115	33	1.381158806586626
115	34	1.4313080385808448
115	35	1.3614212944536135
115	36	3.9055995273037745
115	37	3.861793950260701
115	38	5.1652336066669235
115	39	6.814629831449885
115	40	9.282823441136454
115	41	8.320563491762936
115	42	4.383818707414935
115	43	1.3373777668592588
115	44	0.9560435414719528
115	45	3.474203314147779
115	46	-2.5654159051782024
115	47	-6.03870060406026
115	48	-5.184561801354718
115	49	-7.992460555336594
115	50	-2.0034150158475263
115	51	0.20694663092850618
115	52	0.5995965629958029
115	53	-0.2104919118868871
115	54	0.507786928651079
115	55	0.9822224165625713
115	56	3.5491173445639816
116	1	1.0277455032331773
116	2	2.0595719735043687
116	3	2.5960966760101414
116	4	2.0063555810199247
116	5	1.8370369014488688
116	6	2.3161950850684647
116	7	1.8388555354297438
116	8	1.8193986650375606
116	9	2.0683175897461368
116	10	1.7682863754361975
116	11	-0.5050679484292928
116	12	-2.15112360838506
116	13	-6.628737925464684
116	14	-8.331877211970323
116	15	-4.234998581686501
116	16	-8.627241652238933
116	17	-10.167673066066119
116	18	-2.7820334661376407
116	19	-2.985312054383453
116	20	-3.4500534918300687
116	21	2.4801244053285743
116	22	1.8929137441950417
116	23	1.8637633049395887
116	24	2.5623928138560927
116	25	2.051674593997106
116	26	2.909926138462666
116	27	3.0497788227989844
116	28	2.8657901634439256
116	29	3.2295728585084476
116	30	3.3027844178227497
116	31	1.5637405561764766
116	32	-6.885552851822965
116	33	1.7553651557929726
116	34	1.9023397846125931
116	35	1.5860244690567882
116	36	3.399647146351395
116	37	4.236793950260701
116	38	4.841820908254228
116	39	6.79201078383084
116	40	8.792347250660267
116	41	6.86500793620738
116	42	3.4889774375736664
116	43	-9.432463502982012
116	44	0.902075287503699
116	45	1.8829334728779372
116	46	-5.232479397241693
116	47	-8.740684731044388
116	48	-8.600434817227736
116	49	-6.385317698193736
116	50	-3.7811927936253014
116	51	-0.343053369071491
116	52	-0.1107208973216558
116	53	-1.7870792134741897
116	54	-3.5672130713489203
116	55	-1.6074601231199672
116	56	0.6332443286909637
117	1	0.361829587317267
117	2	0.9694818834142751
117	3	1.7340846639981322
117	4	1.182631857296201
117	5	0.9963462107581833
117	6	1.1411200099933865
117	7	1.2478645444387482
117	8	1.361290556929454
117	9	1.421170442598985
117	10	0.6664845736343992
117	11	-7.69440728776863
117	12	-9.599922407183858
117	13	-8.912071258798019
117	14	-9.17647180656492
117	15	-10.368331915019834
117	16	-13.72724165223893
117	17	-11.1472526456457
117	18	-3.9290304631346373
117	19	-3.2533300724014715
117	20	-3.1761796179561976
117	21	0.7852295104336733
117	22	1.2818026330839274
117	23	0.7958954370717208
117	24	-0.6949645435012641
117	25	1.0294523717748802
117	26	1.7369531654896946
117	27	1.764193237213398
117	28	2.1154898631436296
117	29	1.769062347997938
117	30	1.9985802136185455
117	31	-0.2295026870667627
117	32	-11.34065795692807
117	33	0.2149747654025802
117	34	0.1071445894173948
117	35	-0.21097252794020704
117	36	1.0708183175225692
117	37	1.4597669232336692
117	38	2.041520607953924
117	39	4.497866639686691
117	40	3.51577067408369
117	41	5.126869798069244
117	42	0.9071456057418352
117	43	-7.78006110057961
117	44	-1.963840628412214
117	45	0.397948487892954
117	46	-4.213260178022473
117	47	-6.154048094407752
117	48	-5.397882264675182
117	49	-5.0474798603558995
117	50	-2.442604205036716
117	51	-0.6241344501525745
117	52	0.055195018594258016
117	53	-0.5507428771378535
117	54	-5.574420278556129
117	55	-2.036439102098946
117	56	1.4078689533155888
118	1	1.0130604360003446
118	2	1.8111643301922697
118	3	2.4311639088480774
118	4	1.9566032809491531
118	5	2.1463433416470323
118	6	2.0753599824499176
118	7	1.8312122233278316
118	8	2.004430512171318
118	9	1.9285440582528537
118	10	1.9651016620604054
118	11	-5.001918620757671
118	12	-7.26326090491726
118	13	-6.939672108054918
118	14	0.6495452933375319
118	15	-14.548799006314947
118	16	-5.336583478141268
118	17	-11.105535769533919
118	18	-1.6192238412261055
118	19	-2.3542080204131786
118	20	-2.0819006255880304
118	21	1.4586098971898593
118	22	1.5578111256529326
118	23	1.4932042108136159
118	24	-2.283820915797129
118	25	1.2417665968279579
118	26	2.1833868603310336
118	27	2.5642161901025986
118	28	2.3301567593391823
118	29	2.008518364524015
118	30	2.8637893718213334
118	31	0.688899791845266
118	32	-6.3898345220282025
118	33	1.241600116868696
118	34	1.4686525942375042
118	35	1.0627406757092999
118	36	4.122577082657127
118	37	4.077027495908258
118	38	5.058735274850122
118	39	6.862817577886041
118	40	9.412941730490413
118	41	9.455383024671644
118	42	4.005785647057035
118	43	0.494111160853798
118	44	1.3667957404407147
118	45	4.392841470047081
118	46	-2.837291853009564
118	47	-6.8041312986310825
118	48	-6.480512665776921
118	49	-4.033937655730892
118	50	-2.0580859287986932
118	51	-1.0292529444430443
118	52	-1.5365878470739567
118	53	-3.226074259475606
118	54	-7.080588867527265
118	55	-4.390085742369791
118	56	-1.0037337321724458
119	1	0.7530950249403148
119	2	1.523148132444767
119	3	2.118429273508305
119	4	1.585531445626252
119	5	1.537662361345852
119	6	1.4516623404032671
119	7	1.384550899668156
119	8	1.4892662147064328
119	9	1.5896568097608537
119	10	1.3027234615289132
119	11	-7.224089287649306
119	12	-6.663522431048783
119	13	-1.218914525906186
119	14	-0.9041730177098373
119	15	-7.476499685439258
119	16	-10.950199709634074
119	17	-12.738386826183852
119	18	-1.6178318473002626
119	19	-2.504627727672638
119	20	-2.6858150812340433
119	21	-0.43981672785759685
119	22	-0.5901620468277713
119	23	-0.7939261726174394
119	24	-5.829145081655316
119	25	-0.8425123081368184
119	26	1.381007816166857
119	27	1.5131342311875073
119	28	1.4305068669023555
119	29	1.099293241142739
119	30	1.832659325843352
119	31	-0.2379150729625934
119	32	-12.724883241815604
119	33	0.2845410203993026
119	34	-0.025842702510288973
119	35	0.3872018053334614
119	36	2.0784550933712644
119	37	2.3653811467286907
119	38	3.058524366679535
119	39	5.103379437252471
119	40	7.140912372073071
119	41	6.824978502800466
119	42	2.4578884015177422
119	43	-1.4812125831880465
119	44	1.1934292242218767
119	45	0.07314686507808332
119	46	-7.142560339110716
119	47	-9.336674429351966
119	48	-10.631818187352827
119	49	-7.07295566728866
119	50	-6.427807951829866
119	51	-7.883009218961117
119	52	-7.857041721457049
119	53	-11.230714239228421
119	54	-9.678471349494616
119	55	-11.248777268079497
119	56	-7.502995553575406
120	1	0.7363743448407476
120	2	1.3645365125114637
120	3	1.989595493977049
120	4	1.4619111365754804
120	5	1.5073678707160099
120	6	1.530615888850967
120	7	1.3239619184084646
120	8	1.3772000834772768
120	9	0.8827383935286406
120	10	1.2058750279184665
120	11	-9.634027759303997
120	12	-2.697104695855508
120	13	-5.47944714532284
120	14	-7.099607708424223
120	15	-9.183934751899265
120	16	-10.47192250330276
120	17	-10.692968574340352
120	18	-2.466312425948516
120	19	-2.710489359347992
120	20	-3.3833868251634023
120	21	0.18603457081320585
120	22	0.7782565337931473
120	23	-0.11401447283819266
120	24	-4.79387196155762
120	25	-0.2826043658137678
120	26	1.8163091171860728
120	27	1.9668000993947317
120	28	1.9429769246732391
120	29	2.2913931894777164
120	30	1.8032572310615151
120	31	-1.6174651175823875
120	32	-9.670541031491997
120	33	-2.0943984375876425
120	34	-8.16101718938268
120	35	-2.195063001392384
120	36	0.06276771372728263
120	37	0.33785778004793343
120	38	0.6987949035260925
120	39	1.9895285143272936
120	40	0.31929760527019724
120	41	2.168081222259392
120	42	-2.7338358011970207
120	43	-3.938964685015108
120	44	-5.2630547361369615
120	45	-7.572149269438846
120	46	-11.888744172655406
120	47	-11.603923501729966
120	48	-9.931522287676907
120	49	-15.161913442874589
120	50	-8.345613597407809
120	51	-8.2026278371566
120	52	-7.539207894957592
120	53	-8.84689008817868
120	54	-8.275014489788637
120	55	-8.655568870164885
120	56	-3.921247397077355
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	4403.0
5	672.0
6	298.0
7	399.0
8	765.0
9	662.0
10	385.0
11	294.0
12	293.0
13	405.0
14	463.0
15	706.0
16	881.0
17	1230.0
18	1487.0
19	1774.0
20	2302.0
21	2843.0
22	3449.0
23	4184.0
24	4861.0
25	5272.0
26	5470.0
27	5982.0
28	6476.0
29	6865.0
30	7948.0
31	9104.0
32	11066.0
33	13648.0
34	16104.0
35	19509.0
36	23214.0
37	24958.0
38	14890.0
39	3756.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.71355147861539	0.22171985044778714	0.04830497831106474	0.016423692625762012
8	99.1198832951724	0.4289482074022548	0.05893207353949899	0.3922364238858457
9	97.48516707573971	1.2093423264982703	0.3831436136288967	0.9223469841331193
10	37.757586296843755	36.2852505579225	17.72019824363099	8.236964901602759
11	27.13439670746906	37.667017689624856	20.776372612480316	14.422212990425768
12	28.24875131631066	12.390226936788105	22.46761151204243	36.893410234858806
13	21.96089228955936	11.331381812209566	26.195789738090408	40.511936160140664
14	23.716750018119882	14.238360996351865	33.77062646469039	28.274262520837862
15	32.231260979186125	19.503873868206213	22.23130937229301	26.033555780314654
16	37.128885109014995	15.793644657491882	21.198005257460956	25.879464976032164
17	29.978547408295004	18.546828495226315	30.875787561362145	20.59883653511654
18	28.368122817102805	20.235064514726556	32.77377093528238	18.62304173288826
19	38.471944420287755	23.311688939134804	18.514653448125923	19.701713192451518
20	22.586162744065362	34.9516580258307	22.849494837241437	19.61268439286249
21	21.368495804368095	25.319201356515187	33.64814662731098	19.66415621180574
22	20.234961089400183	19.71180553878259	23.254578214894718	36.79865515692251
23	24.858224888657023	20.704962853471677	22.682085615743556	31.75472664212774
24	20.427209228183056	21.675409867740967	32.618902704112685	25.27847819996329
25	34.07256408893956	26.059212528439694	19.49598342165136	20.372239960969388
26	20.520526333618857	21.012693757652308	22.410797679021666	36.05598222970717
27	19.757345586849695	22.420166118254166	23.607829494445813	34.21465880045032
28	22.71433963359754	32.66798285089374	23.427111459512815	21.190566055995895
29	32.62614207924858	21.873807188516153	27.084249332025568	18.415801400209695
30	21.6498432011133	19.58850560272139	41.40505332128551	17.356597874879803
31	20.320625175515897	30.549933667095974	27.750394608151684	21.379046549236445
32	34.384737648133026	17.33923369389303	28.678747590498226	19.59728106747571
33	25.87555417884731	19.749094921882563	26.446188991926896	27.929161907343232
34	18.570648048312307	35.20725651650461	29.7904951624835	16.43160027269958
35	23.081347020046785	22.49753523168822	39.4295269577993	14.991590790465697
36	19.421665529736764	33.903733484278916	30.38215358713479	16.292447398849525
37	19.5731760467211	22.175572149422283	38.21367347531743	20.03757832853919
38	36.63328495403967	23.119980648282535	25.50798258345428	14.738751814223514
39	22.073745683132202	18.809417562376314	22.899286019168834	36.217550735322654
40	13.247775995333235	16.06387633075689	26.41436974381411	44.27397793009577
41	17.887233295627414	15.960818348480712	29.10978273588953	37.04216562000234
42	26.487248010224828	26.10284862216542	28.179283099982573	19.23062026762718
43	10.736498179720487	12.807884161386806	29.443445297086097	47.012172361806606
44	12.83388697865066	15.874565339860519	20.269246459583893	51.02230122190493
45	17.517228038784243	21.639867495224507	37.699059409531635	23.143845056459607
46	21.089395602005155	21.897644380205637	27.711041393745738	29.301918624043466
47	14.656209680926633	27.805241056716103	28.903127914449872	28.635421347907396
48	18.391965627733665	36.08266356066135	28.020259918515688	17.5051108930893
49	15.092794098401974	19.466645986515896	19.766669736376546	45.673890178705584
50	31.49413046109161	20.928960425995403	29.880672879099603	17.696236233813384
51	22.769512673967103	31.370552962106558	29.610885699189005	16.249048664737334
52	16.949538948093462	26.52612789157883	38.522208586576376	18.002124573751328
53	18.552384456018743	20.64979572869673	42.936182159660774	17.861637655623756
54	19.314878323667266	27.336664457740994	26.30056545272496	27.047891765866776
55	18.101450187019445	35.450951549140385	28.299695639761353	18.147902624078817
56	30.920708684286957	21.93581179204182	27.107173976183564	20.036305547487657
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	2.0
6	11.5
7	21.0
8	76.0
9	131.0
10	262.5
11	394.0
12	472.5
13	623.0
14	695.0
15	784.0
16	873.0
17	981.0
18	1089.0
19	1288.0
20	1487.0
21	1783.5
22	2437.0
23	2794.0
24	3217.0
25	3640.0
26	4026.0
27	4412.0
28	4586.5
29	4671.5
30	4582.0
31	4378.5
32	4175.0
33	4070.5
34	3966.0
35	3811.0
36	3656.0
37	3660.0
38	3924.0
39	4184.0
40	4668.0
41	5152.0
42	7319.0
43	9486.0
44	12663.5
45	15841.0
46	14227.0
47	11347.0
48	10081.0
49	10152.0
50	10223.0
51	10841.0
52	11459.0
53	12111.5
54	13512.0
55	14260.0
56	13466.5
57	12673.0
58	11866.0
59	11059.0
60	9945.5
61	8832.0
62	8038.0
63	6279.0
64	5314.0
65	4778.5
66	4243.0
67	3697.0
68	3151.0
69	2706.0
70	2261.0
71	1809.5
72	1060.5
73	763.0
74	611.0
75	459.0
76	422.5
77	386.0
78	279.5
79	109.5
80	46.0
81	27.5
82	9.0
83	7.0
84	5.0
85	4.0
86	3.0
87	1.5
88	0.5
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.02222029002308978
10	0.0
11	0.0019321991324425896
12	0.0
13	0.0
14	0.030432136335970784
15	0.18210976823271405
16	0.03574568395018791
17	0.02415248915553237
18	0.005313547614217122
19	0.017389792191983305
20	0.026567738071085603
21	0.008211846312881006
22	0.002415248915553237
23	0.0
24	0.0
25	4.830497831106474E-4
26	0.18404196736515666
27	0.027533837637306904
28	0.17389792191983305
29	0.02366943937242172
30	0.03139823590219208
31	0.23379609502555332
32	0.012076244577766185
33	0.19611821194292284
34	0.09516080727279753
35	0.04927107787728604
36	0.1550589803785178
37	0.24925368808509404
38	0.15360983102918588
39	0.4105923156440503
40	0.6318291163087267
41	0.9284216831386644
42	0.22316899979711907
43	0.3531093914538832
44	0.5361852592528186
45	0.11255059946478083
46	0.07294051724970775
47	0.03622873373329855
48	0.05168632679283927
49	0.3386178979605638
50	0.21399105391801682
51	0.35214329188766197
52	0.4149397636920461
53	0.2077114067375784
54	0.13573698905409193
55	0.17148267300427983
56	0.21157580500246356
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	207018.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.70942934567677
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.14917036135289	61.039488811534014
2	6.026828214664665	8.161461983587365
3	1.5574572155673876	3.1636361788912346
4	0.6626087608197906	1.7945944429821619
5	0.38675596978917803	1.3093513005229525
6	0.2435047872956644	0.9892542114437902
7	0.15191725708815	0.7200361549639372
8	0.12208918550846568	0.6613271264045344
9	0.08336867870442398	0.508036109434166
>10	0.5299583445824364	6.818464736485791
>50	0.043666826248109546	2.07839469482782
>100	0.0347349754246326	5.023627138225924
>500	0.002977283607825651	1.6322084428505579
>1k	0.004962139346376085	6.100118667845765
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG	2472	1.1940990638495204	No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG	2129	1.0284129882425683	No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	1913	0.9240742350906683	No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG	1292	0.6241003197789564	No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG	1272	0.6144393241167435	No Hit
ACTTATGGGAATTGACGGGTACGCACAGTTCTGAGTCGGCCACACGCAGGGATGAG	880	0.42508380913736965	No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAACGATTAAG	868	0.41928721174004197	No Hit
ACTTATGGGAATTGACGGGTACGCACAGTTCTGAGTCGGCGACACGCAGGGATGAG	681	0.3289569022983509	No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGCCTCAAAGATTAAG	498	0.24055879198910238	No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTCAG	475	0.22944864697755749	No Hit
ACTTATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC	371	0.17921146953405018	No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG	343	0.16568607560695206	No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAAAGATTAAG	339	0.16375387647450945	No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG	338	0.1632708266913988	No Hit
ACTTATGGGAATTGACGGGTACGCACAGTTCTGAGTCGGACACACGCAGGGATGAG	306	0.14781323363185808	No Hit
ACTTATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	302	0.1458810344994155	No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCAGTCTCAAAGATTAAG	277	0.13380478992164935	No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTAGTCTCAAAGATTAAG	276	0.13332174013853867	No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTCAG	265	0.12800819252432155	No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC	253	0.12221159512699378	No Hit
ACTTATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGCCTCAACGATTAAG	222	0.1072370518505637	No Hit
ACTTATGGGAATTGACGGGTACGCACAGTTCTGAGTCGGAGACACGCAGGGATGAG	222	0.1072370518505637	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0014491493493319421	0.0	0.0	0.0	0.0
10	0.004347448047995826	0.0	0.0	0.0	0.0
11	0.008211846312881006	0.0	0.0	0.0	0.0
12	0.009660995662212948	0.0	0.0	0.0	0.0
13	0.01111014501154489	0.0	0.0	0.0	0.0
14	0.013525393927098128	0.0	0.0	0.0	0.0
15	0.014008443710208774	0.0	0.0	0.0	0.0
16	0.015940642842651363	0.0	0.0	0.0	0.0
17	0.017389792191983305	0.0	0.0	0.0	0.0
18	0.01980504110753654	0.0	0.0	0.0	0.0
19	0.02222029002308978	0.0	0.0	0.0	0.0
20	0.02801688742041755	0.0	0.0	0.0	0.0
21	0.030915186119081433	0.0	0.0	0.0	0.0
22	0.033813484817745314	0.0	0.0	0.0	0.0
23	0.03719483329951985	0.0	0.0	0.0	0.0
24	0.03864398264885179	0.0	0.0	0.0	0.0
25	0.042508380913736966	0.0	0.0	0.0	0.0
26	0.04299143069684762	0.0	0.0	0.0	0.0
27	0.044923629829290206	0.0	0.0	0.0	0.0
28	0.051203277009728626	0.0	0.0	0.0	0.0
29	0.05168632679283927	0.0	0.0	0.0	0.0
30	0.052169376575949916	0.0	0.0	0.0	0.0
31	0.05313547614217121	0.0	0.0	0.0	0.0
32	0.05313547614217121	0.0	0.0	0.0	0.0
33	0.05361852592528186	0.0	0.0	0.0	0.0
34	0.05361852592528186	0.0	0.0	0.0	0.0
35	0.05361852592528186	0.0	0.0	0.0	0.0
36	0.05410157570839251	0.0	0.0	0.0	0.0
37	0.054584625491503155	0.0	0.0	0.0	0.0
38	0.054584625491503155	0.0	0.0	0.0	0.0
39	0.0550676752746138	0.0	0.0	0.0	0.0
40	0.0550676752746138	0.0	0.0	0.0	0.0
41	0.0550676752746138	0.0	0.0	0.0	0.0
42	0.056999874407056395	0.0	0.0	0.0	0.0
43	0.05844902375638834	0.0	0.0	0.0	0.0
44	0.06134732245505222	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCACGT	15	0.0081667155	50.389637	37
TCGCCGA	35	5.044967E-8	50.26676	36
GTCGACG	20	4.077394E-4	50.242264	35
TCGAATC	15	0.008261842	50.24226	43
TGGTAGC	15	0.008261842	50.24226	35
CGATACC	15	0.008285752	50.205555	44
ATATGCG	20	4.121733E-4	50.132294	34
TAGGATT	25	2.049165E-5	50.132294	34
ATACGCT	20	4.121733E-4	50.132294	34
CGATTTA	20	4.1266833E-4	50.12011	49
ACGATTT	20	4.131638E-4	50.107925	48
ACGGATG	200	0.0	50.10792	48
ATTACAG	15	0.00835779	50.09575	45
CCACGAT	240	0.0	50.09575	46
CAACGAT	590	0.0	50.095745	46
AACGTTT	15	0.008365823	50.083576	47
CACGATT	245	0.0	50.083576	47
GGATTAG	15	0.008389956	50.0471	50
GATTAAT	35	5.2221367E-8	50.047096	50
CCCTATG	15	0.008470775	49.92589	32
>>END_MODULE