FastQCFastQC Report
Fri 17 Jun 2016
SRR1526330_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526330_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56847
Sequences flagged as poor quality0
Sequence length56
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG3360.5910602142593276No Hit
CATGATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG2640.4644044540609003No Hit
CATGATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG2640.4644044540609003No Hit
CATGATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG2040.3588579872288775No Hit
CATGATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG1630.2867345682269953No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC1610.2832163526659278No Hit
CATGATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAACGATTAAG1320.23220222703045015No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1110.19526096363924217No Hit
CATGATGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC710.12489665241789363No Hit
CATGATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTCAG670.1178602212957588No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTCCTCTTCC570.10026914349042165No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACATCA150.00802922950.5581441
ACCACTG150.00802922950.5581439
AACCTTA150.00802922950.5581441
TACGCCC150.00802922950.5581438
TGCCCGT354.8035872E-850.46785737
GTACGCC251.951375E-550.46785737
TGCGTGA150.00814353150.37789544
ATGCCCG309.778723E-750.37789536
ATGCTCG150.00814353150.37789536
GCCGGCG150.00817229550.33303542
GCGTTCA150.00817229550.33303542
TATGCTT1600.050.28825435
CGTTCAC150.00823004950.24355743
CCAACAC309.96095E-750.24355743
TAAGAGA150.00823004950.24355734
ATATGCT1800.050.24355734
AATACAG150.00823004950.24355734
CCTCAAA252.003592E-550.24355343
ATATGCG204.0514738E-450.24355334
TCCACGA204.0871988E-450.15439245