##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526330_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 56847 Sequences flagged as poor quality 0 Sequence length 56 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.92638133938466 39.0 38.0 40.0 35.0 40.0 2 36.696254859535244 39.0 38.0 40.0 32.0 40.0 3 37.246644501908634 39.0 38.0 40.0 33.0 40.0 4 37.12023501679948 39.0 38.0 40.0 33.0 40.0 5 36.060143895016445 39.0 36.0 39.0 29.0 40.0 6 37.05922915897057 39.0 38.0 40.0 33.0 40.0 7 37.60423593153553 39.0 38.0 40.0 35.0 40.0 8 37.29774658293314 39.0 38.0 40.0 33.0 40.0 9 37.34652664168733 39.0 38.0 40.0 34.0 40.0 10 36.7452284201453 39.0 36.0 40.0 32.0 40.0 11 35.40383837317712 38.0 34.0 39.0 28.0 40.0 12 33.21017819761817 37.0 31.0 39.0 19.0 40.0 13 32.776980315583934 37.0 30.0 39.0 17.0 40.0 14 31.96668249863669 36.0 28.0 39.0 14.0 40.0 15 32.69111826481608 37.0 30.0 39.0 17.0 40.0 16 32.47601456541242 36.0 30.0 39.0 17.0 40.0 17 32.59355814730768 36.0 30.0 39.0 19.0 40.0 18 32.865076433233064 36.0 30.0 39.0 20.0 40.0 19 33.690203528770205 36.0 31.0 39.0 24.0 40.0 20 33.85355427727057 37.0 31.0 39.0 24.0 40.0 21 35.38895632135381 38.0 35.0 39.0 27.0 40.0 22 35.05395183562897 38.0 34.0 39.0 26.0 40.0 23 35.26124509648706 38.0 35.0 39.0 27.0 40.0 24 34.828715675409434 38.0 34.0 39.0 25.0 40.0 25 35.15045648846905 38.0 35.0 39.0 27.0 40.0 26 35.55561419248157 38.0 35.0 39.0 29.0 40.0 27 35.56001196193291 38.0 35.0 39.0 29.0 40.0 28 35.321371400425704 38.0 35.0 39.0 28.0 40.0 29 35.26903794395483 38.0 35.0 39.0 28.0 40.0 30 34.9673509595933 38.0 35.0 39.0 27.0 40.0 31 34.71857793727022 38.0 35.0 39.0 26.0 40.0 32 33.567488170000175 38.0 33.0 39.0 19.0 40.0 33 34.87188418034373 38.0 35.0 39.0 27.0 40.0 34 34.623973120833114 38.0 35.0 39.0 26.0 40.0 35 34.69929811599557 38.0 35.0 39.0 27.0 40.0 36 32.67866378172991 36.0 31.0 38.0 23.0 39.0 37 32.49298995549457 35.0 31.0 38.0 23.0 39.0 38 31.747409713793164 35.0 31.0 38.0 17.0 39.0 39 29.75569511143948 34.0 28.0 38.0 7.0 39.0 40 25.328548560170283 31.0 12.0 37.0 4.0 39.0 41 22.030502928914455 26.0 9.0 33.0 4.0 37.0 42 26.0655971291361 30.0 22.0 34.0 4.0 37.0 43 21.67876932819674 26.0 8.0 33.0 4.0 36.0 44 22.954474290639787 28.0 10.0 33.0 4.0 37.0 45 25.71289601913909 30.0 22.0 34.0 4.0 37.0 46 25.509085791686456 30.0 17.0 36.0 4.0 38.0 47 24.791967913874082 30.0 11.0 36.0 4.0 38.0 48 24.869597340229035 30.0 13.0 35.0 4.0 38.0 49 22.11121079388534 26.0 5.0 35.0 4.0 38.0 50 24.713458933628864 30.0 12.0 35.0 4.0 38.0 51 26.260066494274103 32.0 4.0 38.0 4.0 40.0 52 26.10262634791634 32.0 4.0 38.0 4.0 40.0 53 25.666824986366915 32.0 4.0 38.0 4.0 40.0 54 24.69966752862948 31.0 4.0 38.0 4.0 40.0 55 24.947367495206432 31.0 4.0 38.0 4.0 39.0 56 24.194416591904584 31.0 4.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.3605766406199251 1 2 1.8738291882780729 1 3 1.332811074229987 1 4 1.167692230398309 1 5 2.1424605309419036 1 6 1.2210370543879847 1 7 0.6429884916067934 1 8 0.9043931653593944 1 9 0.6841898277843583 1 10 1.2490782767771833 1 11 -4.076833265621907 1 12 -8.538889750175947 1 13 -21.89101026334727 1 14 -17.629211066154017 1 15 -11.893163562688205 1 16 -20.859227493085005 1 17 -21.435318034418383 1 18 -21.015755700453873 1 19 -6.408995044111684 1 20 -10.526688093486687 1 21 -7.918393980467474 1 22 -3.606772858009183 1 23 -4.432208800748036 1 24 -3.5430127292831735 1 25 -4.079797198428828 1 26 1.6371081048359883 1 27 1.8273725035039803 1 28 1.5669150445275477 1 29 1.548193976162736 1 30 2.1332294506463896 1 31 0.6922195775621631 1 32 -10.061097756145173 1 33 1.1375431152230817 1 34 1.2374009735721216 1 35 1.010317949184433 1 36 3.917844288071727 1 37 3.809118511124513 1 38 5.1881502884552155 1 39 7.416586329098251 1 40 10.33717459063783 1 41 -2.3713559322866935 1 42 -8.947865562374417 1 43 -13.039513481736819 1 44 -15.58806715461531 1 45 -13.98579458873069 1 46 -17.79048680904593 1 47 -17.26918298333612 1 48 -16.56780560994323 1 49 -13.967766586136705 1 50 -15.810659182259176 1 51 -17.402565790606072 1 52 -16.509726550339717 1 53 -16.617465679454657 1 54 -18.072387695718334 1 55 -16.407679628724054 1 56 -15.503935159582882 2 1 0.6108572242338397 2 2 2.079216393665277 2 3 1.328321736407318 2 4 1.1727427354488142 2 5 2.2642338193818645 2 6 1.4471874472050459 2 7 0.9662208148391187 2 8 0.9245951855614152 2 9 1.086546730141258 2 10 1.2170917447906504 2 11 -3.4410307964861033 2 12 -11.896353274306136 2 13 -13.526812732483073 2 14 -16.67578794606423 2 15 -8.292153461678105 2 16 -3.649350949875128 2 17 -3.0868331859335356 2 18 -10.864801716166554 2 19 -2.5818345502845226 2 20 -3.7618171619490894 2 21 1.8638731351329767 2 22 1.9566390387360428 2 23 2.233335531462963 2 24 1.8582218386180571 2 25 2.254097302132344 2 26 1.290867925262475 2 27 1.3582367010348477 2 28 1.546713024325527 2 29 1.364131125433218 2 30 1.6972025146194554 2 31 -2.804413419070837 2 32 -9.277708306089057 2 33 -1.8955657512191166 2 34 -1.6728122699744574 2 35 -1.5991096602431796 2 36 3.604712974940419 2 37 3.940431642437641 2 38 4.398026831665089 2 39 6.999077911589829 2 40 9.730552817349391 2 41 7.904177176579751 2 42 2.8456248977827094 2 43 -0.911006186562858 2 44 -9.016798916680404 2 45 0.8862592833231844 2 46 -11.463887482446605 2 47 -11.099710480530284 2 48 -9.435931311402262 2 49 -12.110303062006514 2 50 -9.104710809644136 2 51 -8.180904735611684 2 52 -7.94238648299965 2 53 -9.539463434785745 2 54 -14.814250770914743 2 55 -9.140564028275119 2 56 -8.439400928381984 3 1 1.2097662107397227 3 2 2.592997616748825 3 3 2.0403515957239975 3 4 1.6740347251128966 3 5 2.675230086967275 3 6 1.6717065399411908 3 7 1.587667843262885 3 8 1.7165848496441 3 9 1.565444232294574 3 10 1.9724750350576628 3 11 1.5045620832153048 3 12 1.7757684598310988 3 13 -4.995661426137968 3 14 -7.032664202165293 3 15 -2.08801909475304 3 16 2.01286553017081 3 17 2.8201435582525107 3 18 2.235829923224774 3 19 0.4747258861208721 3 20 1.046250595731074 3 21 2.9709647228447196 3 22 3.7364265782135107 3 23 3.2911879575324434 3 24 3.534506650561788 3 25 3.3511400813456618 3 26 2.8999692746739 3 27 2.5327988600931306 3 28 2.5803047555916763 3 29 2.9061925667194615 3 30 2.7058157790466737 3 31 3.6576882174494045 3 32 3.3219184524524294 3 33 3.417669965117369 3 34 3.342404497180297 3 35 3.0201840520737946 3 36 5.702904189410702 3 37 6.322860583006115 3 38 6.657142536517231 3 39 9.60932769625822 3 40 12.292424766818236 3 41 10.986290297452559 3 42 8.912951914722132 3 43 2.540472423830483 3 44 3.4239131131789122 3 45 7.171358335864095 3 46 0.5692735855981823 3 47 -3.630000460432665 3 48 0.45439312155783185 3 49 -8.732037199244527 3 50 0.630144200404672 3 51 0.18587677458068086 3 52 0.5748400458547849 3 53 0.7518084572613404 3 54 -2.356742875422352 3 55 0.5479229082738328 3 56 4.701138836188786 4 1 1.0467452574816107 4 2 2.264768282187461 4 3 1.8623640295255157 4 4 1.6919946606413347 4 5 2.1294092258113935 4 6 1.6475297036529142 4 7 1.3006542581834495 4 8 1.2755302790707645 4 9 1.6879402028218635 4 10 1.6809714660961177 4 11 3.353859803685026 4 12 3.244338572656339 4 13 4.8961184495240175 4 14 3.484950349987596 4 15 5.502125966264689 4 16 6.309089338598167 4 17 5.546830180403099 4 18 5.299725847700945 4 19 3.670212876680395 4 20 4.04981955727839 4 21 2.3573335922898053 4 22 3.097697589035519 4 23 2.6680012202540624 4 24 3.5279443664263965 4 25 2.644025183809397 4 26 1.9594903430598123 4 27 0.35872563881751063 4 28 1.5423125842815253 4 29 1.289568113576479 4 30 1.8849546231636367 4 31 2.442744630067409 4 32 3.7546838220126375 4 33 2.072286589570517 4 34 2.270104243899155 4 35 2.153732290618578 4 36 3.701522655908512 4 37 3.926130212294627 4 38 4.597391212547624 4 39 7.08745341580692 4 40 9.921483021480917 4 41 5.500681271433681 4 42 6.966025826764497 4 43 7.5177922488362405 4 44 10.424834135513706 4 45 7.391943185046689 4 46 7.35202744237921 4 47 7.3515790928715 4 48 6.3390350741251815 4 49 3.8414143319551037 4 50 6.833805264185468 4 51 4.11783624959795 4 52 3.8291573380491215 4 53 3.69021508862215 4 54 -1.335213978346598 4 55 4.2142825674955695 4 56 3.0590711410471805 5 1 1.4776090335143834 5 2 2.828897108863238 5 3 2.5062697994128484 5 4 2.0846201300848293 5 5 3.2161282425406554 5 6 2.2445480261748187 5 7 1.6597074048774303 5 8 1.824978327323869 5 9 1.5028941120058832 5 10 2.2694544523257747 5 11 3.4001782286309634 5 12 6.264140552854357 5 13 6.792897782629744 5 14 6.305480678882557 5 15 6.520107074720361 5 16 7.267209288524189 5 17 6.591327733606697 5 18 6.5856027112493685 5 19 5.062269323704406 5 20 5.290971258655425 5 21 3.839181777108287 5 22 3.95749046487483 5 23 3.1111558805476847 5 24 4.117906810946714 5 25 3.619784828739405 5 26 3.0524686064033872 5 27 2.6549587104005354 5 28 2.9566747101492794 5 29 2.257702858085409 5 30 2.603971352422775 5 31 3.182815188847428 5 32 4.57840067645671 5 33 2.9771460410329027 5 34 2.8601804929033605 5 35 3.057339892758101 5 36 4.654521404059189 5 37 5.132002523663697 5 38 6.257115805696589 5 39 7.973535127426359 5 40 7.74417563556991 5 41 3.5716952693851987 5 42 9.46801740523269 5 43 13.750977636340505 5 44 12.586436502646972 5 45 9.992261837601596 5 46 11.343264497119165 5 47 11.08504899158551 5 48 11.316729395281435 5 49 10.234950237269775 5 50 11.458933294229855 5 51 9.943829193719946 5 52 10.51482935352653 5 53 9.946275177389648 5 54 9.988218364887729 5 55 11.237043464274901 5 56 10.902020953269783 6 1 1.5591506156644854 6 2 2.4456651954081963 6 3 2.2222941401836422 6 4 2.0890826047298603 6 5 2.3921931512627594 6 6 1.6500923128550085 6 7 1.6551097037280016 6 8 1.5821115254306974 6 9 1.947113178942999 6 10 1.6332137491479486 6 11 3.964343204966326 6 12 6.511199376383772 6 13 5.820754442535083 6 14 7.421234566644561 6 15 6.924840002374186 6 16 6.881948977503232 6 17 6.524931519948815 6 18 6.289591893129018 6 19 5.214534367653023 6 20 5.301654152502614 6 21 3.4234955025984775 6 22 3.604278835395448 6 23 3.1578090245639103 6 24 3.831497074638392 6 25 3.3467625163661836 6 26 2.5791758410213745 6 27 2.5842352486020204 6 28 2.1480202138680085 6 29 3.064058503791969 6 30 3.327703603944073 6 31 3.6496170820184872 6 32 4.553518999648048 6 33 3.1038532756508914 6 34 2.883033772146092 6 35 2.5233304269027954 6 36 3.1641900991234237 6 37 3.082915302568363 6 38 4.643052249239528 6 39 4.624785972592008 6 40 3.9123974070371155 6 41 1.845934620297978 6 42 7.50953194208191 6 43 12.207367088673163 6 44 9.928153879252783 6 45 7.31937475173277 6 46 9.824129946747291 6 47 10.544007747501666 6 48 10.41882540609955 6 49 10.88998742455848 6 50 9.781854186724782 6 51 9.586560769108726 6 52 9.33038040153194 6 53 9.51747193195354 6 54 10.17253209037792 6 55 9.59552892742568 6 56 10.013177139882359 7 1 1.413326360036308 7 2 2.4372410850233024 7 3 1.7357291438147229 7 4 1.580150142856219 7 5 2.5728757946905034 7 6 2.1632368299210967 7 7 1.6081961234810933 7 8 1.7394100003762318 7 9 1.6667936437215047 7 10 1.859067053432625 7 11 3.163907475118833 7 12 5.68389363927411 7 13 5.584298378628041 7 14 7.126681189738239 7 15 6.18932801980338 7 16 6.585216951359438 7 17 6.9502038511035025 7 18 6.332729148030975 7 19 4.911992610209303 7 20 5.090034689902765 7 21 3.2342435055033434 7 22 3.0800958288595055 7 23 2.78889108701852 7 24 3.685382332445222 7 25 2.8713812527496216 7 26 2.7970407647686457 7 27 2.197742873874354 7 28 2.5714043823502166 7 29 3.709810137778895 7 30 2.758930909681183 7 31 2.899290284632869 7 32 3.8210571260097055 7 33 2.2031996808796492 7 34 2.1790395818773973 7 35 2.277433549633365 7 36 1.2713796416070835 7 37 0.9774686794746792 7 38 1.9782737452453354 7 39 4.233645812824399 7 40 -2.4793237258604854 7 41 -8.392119119716547 7 42 5.018464403955548 7 43 9.49640122084455 7 44 8.106657873443051 7 45 4.886259283323184 7 46 8.26450757928179 7 47 8.517573470087001 7 48 8.58876004662243 7 49 10.926733975030523 7 50 7.64837561010895 7 51 9.066008844635231 7 52 9.773662899716399 7 53 8.96670940472043 7 54 10.420317130319823 7 55 9.563139675428584 7 56 9.140845985198261 8 1 1.5627247035274792 8 2 2.5846078610820626 8 3 2.246121392603598 8 4 1.7747117931140721 8 5 2.8726101281801135 8 6 2.3063841966971523 8 7 1.6905527417026889 8 8 1.805045255884906 8 9 1.338773640596017 8 10 1.8350007930794447 8 11 3.499116101466697 8 12 5.821102578170816 8 13 6.608989736652731 8 14 7.436573360389904 8 15 6.375919674749465 8 16 7.128114279066896 8 17 6.508103522927222 8 18 6.071125772503553 8 19 5.173283436900974 8 20 4.615429282807902 8 21 3.1007851526357086 8 22 3.4246809202800392 8 23 2.9195365003643587 8 24 4.087788958480537 8 25 2.849815383082486 8 26 2.547157970582056 8 27 2.464503305191748 8 28 2.729554093242548 8 29 3.145815763657943 8 30 1.8706671184637997 8 31 2.609557513887438 8 32 3.433340295254901 8 33 2.0794303419204354 8 34 2.9585363782518286 8 35 2.138818140975765 8 36 0.7720828764875307 8 37 0.7435258759116188 8 38 2.1965891070206105 8 39 3.232083068645622 8 40 -2.822971170773755 8 41 -8.475882207699044 8 42 5.659345791672379 8 43 10.056957557772197 8 44 9.791296098165667 8 45 6.298823746520558 8 46 9.08369807779718 8 47 9.933732244895566 8 48 9.971319821587265 8 49 12.38321154965156 8 50 10.038592831549803 8 51 10.887230910583032 8 52 11.050112344942214 8 53 11.037501716019069 8 54 12.161214145478446 8 55 11.36217077403774 8 56 10.253832389323904 9 1 1.369234649277928 9 2 3.0448248592737457 9 3 2.527616269035178 9 4 1.9306792433853204 9 5 3.090512478993851 9 6 2.270820604171533 9 7 1.6765382751565738 9 8 1.8849126458788774 9 9 1.7902504338449603 9 10 2.1906367183356252 9 11 3.387452448663808 9 12 6.668902457616262 9 13 6.858989736652731 9 14 7.3383214013784475 9 15 7.140386220861576 9 16 7.378867745010236 9 17 6.925071575971224 9 18 6.154157719459551 9 19 5.50767162255498 9 20 5.872662555863961 9 21 3.684419872346382 9 22 3.909460908224581 9 23 3.021254402715172 9 24 4.706987270716823 9 25 3.247042628411002 9 26 2.09598256371045 9 27 2.0773725035039803 9 28 2.4197288973414004 9 29 2.511397439366199 9 30 2.9286839961009363 9 31 3.796115681458261 9 32 5.266824321776902 9 33 3.234945712625681 9 34 3.3055827917539347 9 35 3.2289326677991497 9 36 2.8269351971626406 9 37 3.3491618011677993 9 38 4.271483621788544 9 39 5.712040874552791 9 40 0.10665511011834994 9 41 -2.726334287265047 9 42 7.620316255807403 9 43 11.878235436012101 9 44 10.396781330233175 9 45 8.601867748931646 9 46 11.085054316495196 9 47 12.50787329372016 9 48 11.739553697416078 9 49 14.610155491785374 9 50 11.449081077481086 9 51 12.032499144458864 9 52 11.818195527582361 9 53 11.956127394138417 9 54 12.512027888697247 9 55 11.275220804176382 9 56 11.558835403187679 10 1 1.4608097408530227 10 2 2.654714969163855 10 3 2.2675430089619226 10 4 1.9590675217735978 10 5 2.8872157756971504 10 6 1.8807107140616424 10 7 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3.451048672339944 114 37 3.420337079789885 114 38 4.620774401930394 114 39 6.93367208022655 114 40 10.21177162480933 114 41 9.831416472613373 114 42 6.607904908289672 114 43 2.109745061138746 114 44 2.499618209665801 114 45 5.6963459657502895 114 46 -2.965857537608148 114 47 -6.037719411447014 114 48 -4.288308511297192 114 49 -12.30625788207481 114 50 -2.9035025091450564 114 51 -2.57439041987751 114 52 -1.4758774207459062 114 53 -1.9253315724269306 114 54 -3.0685192237647563 114 55 -1.8390142515480363 114 56 0.8587340860650876 115 1 -0.4656859856427005 115 2 1.0273645418134265 115 3 0.6419019833208992 115 4 0.43940940211547996 115 5 0.3111473996287728 115 6 -0.002195268844339182 115 7 -0.25600140738310984 115 8 0.1690396300058623 115 9 -0.12086067726615113 115 10 0.34301767071657707 115 11 -0.6434999322885773 115 12 -4.758081669367865 115 13 -1.702121374458379 115 14 -6.1473928843358365 115 15 -4.069931239455883 115 16 -12.748116381973894 115 17 -9.5534998526002 115 18 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-1.1554503110980328 116 1 0.7620917921350738 116 2 1.729889794338682 116 3 1.5525080439269558 116 4 0.7131467758528558 116 5 1.8015514400328172 116 6 1.0998249331758672 116 7 0.8323824310007311 116 8 0.5331810441472697 116 9 0.9872201308146629 116 10 0.4915025192014255 116 11 -0.8268332656219073 116 12 -2.690404901691096 116 13 -6.481919354256359 116 14 -8.750423187366138 116 15 -4.4764968960215406 116 16 -9.359227493085005 116 17 -9.495924095024446 116 18 -4.33393751863569 116 19 -4.522631407748047 116 20 -4.526688093486687 116 21 1.3240302619567714 116 22 1.3098938086574847 116 23 1.5829427144034796 116 24 2.313047876777432 116 25 1.9050512864196563 116 26 2.7128656805935663 116 27 2.478887655019136 116 28 1.5972180748305789 116 29 1.64667882464758 116 30 2.618077935494874 116 31 0.6164620018045852 116 32 -5.0004916955391145 116 33 1.0693612970412616 116 34 1.4798252159963639 116 35 1.798196737063222 116 36 4.698147318374758 116 37 5.240936692942693 116 38 5.491180591485513 116 39 8.18173784424976 116 40 10.322023075486314 116 41 6.5756137646830055 116 42 2.612740498231645 116 43 -9.054664996888333 116 44 -1.1183701849183407 116 45 1.3626902597541601 116 46 -5.138971657530782 116 47 -7.534334498487635 116 48 -6.885987428125045 116 49 -6.195039313409433 116 50 -2.7879319095319026 116 51 -0.23589912393940438 116 52 -0.5779083685215376 116 53 -3.4886778006667782 116 54 -4.61784224117288 116 55 -3.407679628724054 116 56 -0.9584806141283373 117 1 -0.03629530463911834 117 2 1.2130276242506994 117 3 0.8655578972993894 117 4 0.6530294738000677 117 5 1.8624018799155166 117 6 0.7669803583958057 117 7 0.2099484134054208 117 8 0.3081077303642843 117 9 -0.8119001037894478 117 10 0.8096843373832456 117 11 -11.1252203623961 117 12 -13.316504608435967 117 13 -9.810365102056945 117 14 -12.839866002615405 117 15 -10.342332673147638 117 16 -11.859227493085005 117 17 -11.635220282707728 117 18 -5.592002034764722 117 19 -6.021898269918136 117 20 -5.598535600818064 117 21 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1.330790872209782 118 4 0.9727427354488114 118 5 1.933369621851 118 6 0.6200269533778879 118 7 0.38844303706133587 118 8 0.5690396300058609 118 9 0.7013615449560717 118 10 -0.25698232928342435 118 11 -6.999055487844128 118 12 -9.11363722492342 118 13 -7.924343596680604 118 14 -0.16961510655805867 118 15 -18.269931239455882 118 16 -7.348116381973892 118 17 -12.909055408155758 118 18 -3.6006041853023554 118 19 -4.031217266333908 118 20 -4.974162840961434 118 21 0.41695955488606273 118 22 0.9023180510817213 118 23 -1.104936073475308 118 24 -3.0874571737276177 118 25 0.2565664379348078 118 26 1.078522246250131 118 27 1.094039170170646 118 28 0.6800463576588598 118 29 1.3542545822233407 118 30 1.134239551656492 118 31 0.4104013957439818 118 32 -12.097461392508812 118 33 -0.6190225413425736 118 34 0.05558279175393466 118 35 -2.3472578083913263 118 36 0.7158240860515264 118 37 0.2737649757709768 118 38 0.7437058440107691 118 39 4.050929763441683 118 40 6.661417014880254 118 41 6.427633966703208 118 42 0.536982922474067 118 43 -1.4344629766863122 118 44 -1.7254408919890452 118 45 1.2788518759157768 118 46 -4.315739334298456 118 47 -7.976253690406827 118 48 -6.744573286710903 118 49 -7.448574666944786 118 50 -3.554093525693517 118 51 -5.2500405380808175 118 52 -4.312756853370022 118 53 -8.0629202249092 118 54 -10.567337190667828 118 55 -8.720810941855365 118 56 -6.622116977764701 119 1 0.39014057262287594 119 2 1.3601558697267038 119 3 0.9898694629956921 119 4 1.029111299134449 119 5 1.294345231607096 119 6 0.7528182812911624 119 7 0.3429145817767818 119 8 0.6590125297348592 119 9 0.17236425498317232 119 10 0.6145623861637333 119 11 -9.113418631475565 119 12 -9.296835056901742 119 13 -2.6349127023716576 119 14 -2.662840038807378 119 15 -6.717627716420651 119 16 -7.908007980889881 119 17 -9.062442942031097 119 18 -1.4802789820503222 119 19 -1.7260682148433908 119 20 -1.4191492908259349 119 21 -1.0307369220787095 119 22 0.31044813238253965 119 23 0.11620213790680367 119 24 -6.197484273998622 119 25 -0.8865229929594989 119 26 -0.02771081608049286 119 27 0.5021692514714644 119 28 0.1537590947862313 119 29 -0.07338769419942537 119 30 -0.30099080064703543 119 31 -1.2416311245812253 119 32 -11.705591473809623 119 33 -1.029862649743663 119 34 -0.3427911919859028 119 35 -0.21067244253766404 119 36 2.493601863829305 119 37 1.8401606397276176 119 38 3.5133535404877314 119 39 5.173422988373929 119 40 7.497189372603831 119 41 7.599449684860389 119 42 2.642673979384636 119 43 -0.1455740877974243 119 44 0.8073504359242314 119 45 -0.6051589641926256 119 46 -7.264248819393307 119 47 -9.20118593972932 119 48 -8.693082771805754 119 49 -7.945593637134488 119 50 -6.045692441682679 119 51 -9.189335931034748 119 52 -9.060724333044817 119 53 -11.719830794014893 119 54 -11.125602773323656 119 55 -12.644930183047778 119 56 -10.480653563130554 120 1 -0.187908207864929 120 2 1.2662534307023137 120 3 0.5502353166542306 120 4 0.09496495767103852 120 5 1.3333696218509985 120 6 0.08391584226677651 120 7 -0.369890296271997 120 8 -0.15318259221636055 120 9 -0.3514162328217054 120 10 -0.06531566261675437 120 11 -8.451833265621907 120 12 -2.6608594471456435 120 13 -5.966010263347268 120 14 -4.314059551002501 120 15 -6.206042350566992 120 16 -10.409227493085005 120 17 -11.586833185933536 120 18 -3.5089375186356904 120 19 -3.8589950441116834 120 20 -4.563051729850322 120 21 -0.6691515562250459 120 22 0.2939847177483941 120 23 -1.3966027401419723 120 24 -5.434679395949843 120 25 -1.7017668953985208 120 26 0.7174111351390167 120 27 1.7107058368373131 120 28 1.3994908021033083 120 29 0.16258791555667074 120 30 0.820350662767602 120 31 -2.531265270922688 120 32 -10.564128059175477 120 33 -1.907911430231465 120 34 -8.561083874912729 120 35 -3.7972578083913255 120 36 -1.6813981361706958 120 37 -0.9317905797845789 120 38 0.3798169551218784 120 39 -0.2546257921138739 120 40 -5.730249651786412 120 41 -2.808477144407906 120 42 -6.6213504108592645 120 43 -6.870574087797424 120 44 -6.636552003100157 120 45 -10.035037012973113 120 46 -13.043517112076234 120 47 -13.520698134851271 120 48 -11.22235106448868 120 49 -14.579130222500341 120 50 -10.01520463680463 120 51 -10.547262760303042 120 52 -10.071090186703355 120 53 -10.904586891575867 120 54 -12.595114968445607 120 55 -11.548588719633145 120 56 -8.4471169777647 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 391.0 5 34.0 6 48.0 7 78.0 8 116.0 9 147.0 10 118.0 11 83.0 12 80.0 13 92.0 14 133.0 15 202.0 16 261.0 17 351.0 18 456.0 19 549.0 20 686.0 21 855.0 22 1114.0 23 1240.0 24 1481.0 25 1635.0 26 1771.0 27 1820.0 28 1929.0 29 2148.0 30 2297.0 31 2677.0 32 3166.0 33 3770.0 34 4514.0 35 5562.0 36 6353.0 37 6244.0 38 3463.0 39 983.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.46874945027882 0.44153605291396203 0.05805055675761254 0.03166394004960684 8 99.3526483367636 0.4450542684750295 0.05453234119654511 0.1477650535648319 9 97.86219516486611 1.1084913959953548 0.39237076397930815 0.6369426751592357 10 49.606839411050714 29.4703326472813 12.396432529421078 8.526395412246908 11 33.55557119234423 31.60116806811385 18.018857967139287 16.82440277240263 12 33.49165303358137 12.757049624430488 20.236775907259837 33.51452143472831 13 24.90368884901578 12.1888578113181 22.783963973472655 40.12348936619347 14 22.38351632882883 14.991554054054054 30.651745495495497 31.97318412162162 15 30.56588477873923 15.628359445217912 24.28845848827168 29.51729728777118 16 33.88589730053145 16.30063703234435 23.9767008059691 25.836764861155103 17 27.973959707926454 20.01231635435911 27.43907803290226 24.574645904812176 18 27.775725268732078 21.998205520662903 27.327105434457522 22.8989637761475 19 31.892319873317497 24.979326119468638 18.69446643793437 24.433887569279495 20 27.363105292792795 28.374859234234233 19.596354166666664 24.665681306306304 21 26.099577762139337 22.76389866291344 27.350457424349052 23.78606615059817 22 25.3276338241244 21.10049782750189 23.88516544408676 29.686702904286943 23 27.837880626946017 21.89209632874206 23.244850211972487 27.02517283233944 24 27.336534909493903 20.69238482241807 27.91704047707003 24.054039791017995 25 31.855682797685013 21.364363994581947 21.94311045437754 24.836842753355498 26 23.080176956835928 21.085006256939913 26.2579974267233 29.576819359500856 27 22.802139790244247 17.670866474273243 26.451749137748997 33.07524459773351 28 21.347007824064285 27.801860858532457 26.709311341368856 24.141819976034398 29 30.065980469780946 20.300870942201108 27.699480953637725 21.933667634380225 30 22.42653528066162 19.929614640154846 36.83265880696815 20.81119127221538 31 23.380470430581514 25.36058116161794 30.92181824593575 20.337130161864795 32 31.355887475589807 16.653471965658593 30.555408947766576 21.435231610985028 33 24.876191818966884 18.19671842230486 32.743518795932395 24.183570962795862 34 21.675822241002887 24.080921191633216 36.80893020635256 17.434326361011337 35 21.276445946421656 21.753441053261522 40.88956947231316 16.08054352800366 36 19.883360349566566 29.011910634998944 33.33744449925999 17.7672845161745 37 21.68300768850956 22.040981872046274 38.756436481625165 17.519573957819002 38 27.388355500748702 23.988373117237735 32.65040077512552 15.972870606888048 39 21.92753213730753 18.600791072185338 29.14606582850685 30.325610962000283 40 13.864802689789418 14.781454609803573 27.688904618651566 43.66483808175544 41 15.97919846650811 16.039544212133045 30.45507791700685 37.52617940435199 42 23.040857334226388 22.00267916945747 33.85800401875419 21.098459477561956 43 11.81214923581942 12.401609544315415 30.193074723800784 45.59316649606438 44 12.916858223998304 14.002546238992823 25.050394313399583 48.03020122360929 45 19.829514433153104 19.966889166769406 40.71223515736452 19.491361242712976 46 19.044601682683844 21.78336325553561 35.0582602879572 24.113774773823355 47 16.97960476534042 23.235433860664827 33.095183627501015 26.689777746493743 48 19.58882650097689 25.09284846778026 36.841919983102464 18.47640504814039 49 14.307646155746529 16.645785173551676 24.810743087049357 44.235825583652435 50 22.82545448133585 19.82966868266535 37.700350889566764 19.644525946432033 51 19.75886174269171 24.68224827001836 36.97041378336393 18.588476203926 52 17.932191417976338 21.199011124845487 39.91523927247042 20.953558184707752 53 18.19287300412393 21.400373620950973 41.7503789080399 18.6563744668852 54 16.88034940650206 22.65858898946849 33.22884012539185 27.232221478637598 55 18.39930940929831 25.388016841956905 35.8420097598788 20.370663988865985 56 23.931202199273958 20.8261375251119 33.94600500475804 21.296655270856096 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 0.5 9 0.0 10 10.5 11 21.0 12 33.5 13 80.0 14 114.0 15 148.0 16 182.0 17 237.5 18 293.0 19 339.5 20 386.0 21 455.0 22 622.5 23 721.0 24 906.0 25 1091.0 26 1296.5 27 1502.0 28 1614.5 29 1801.0 30 1875.0 31 1816.0 32 1757.0 33 1609.5 34 1462.0 35 1328.5 36 1195.0 37 1081.5 38 942.0 39 916.0 40 902.0 41 888.0 42 961.0 43 1034.0 44 1354.5 45 1675.0 46 2146.0 47 2428.5 48 2240.0 49 2145.5 50 2051.0 51 2171.5 52 2292.0 53 2418.0 54 2615.0 55 2686.0 56 2909.5 57 3133.0 58 3325.5 59 3518.0 60 3543.5 61 3569.0 62 3527.0 63 3196.0 64 2907.0 65 2580.0 66 2253.0 67 1946.0 68 1639.0 69 1426.5 70 1214.0 71 1076.5 72 777.0 73 615.0 74 492.5 75 370.0 76 287.0 77 204.0 78 163.0 79 88.5 80 55.0 81 30.5 82 6.0 83 7.0 84 8.0 85 4.5 86 1.0 87 0.5 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.022868401146938274 10 0.0 11 0.0017591077805337132 12 0.0 13 0.0 14 0.0263866167080057 15 0.1829472091755062 16 0.03694126339120798 17 0.02110929336640456 18 0.01055464668320228 19 0.02110929336640456 20 0.0263866167080057 21 0.012313754463735992 22 0.0 23 0.0 24 0.0 25 0.0 26 0.19350185585870847 27 0.03342304783014055 28 0.17415167027283762 29 0.02110929336640456 30 0.029904832269073128 31 0.23396133481098386 32 0.01055464668320228 33 0.1864654247365736 34 0.08971449680721938 35 0.05805055675761254 36 0.16007880802856791 37 0.24451598149418616 38 0.1442468380037645 39 0.3782081728147484 40 0.5928193220398613 41 0.8883494291695252 42 0.1987791792003096 43 0.32543493939873697 44 0.515418579696378 45 0.11961932907629251 46 0.05805055675761254 47 0.03518215561067427 48 0.059809664538146255 49 0.31312118493500096 50 0.2357204425915176 51 0.350062448326209 52 0.3817263883758158 53 0.1847063169560399 54 0.11434200573469137 55 0.1477650535648319 56 0.17766988583390503 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 56847.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.85584111738527 #Duplication Level Percentage of deduplicated Percentage of total 1 93.39524855922716 79.25132372860485 2 4.363779592852108 7.405843756046933 3 0.9950661304365853 2.5331152039685474 4 0.47058335751896846 1.5972698647246117 5 0.2591318048011941 1.0994423628335708 6 0.08914134085161077 0.45384980737769803 7 0.11401799411252539 0.6772564955054796 8 0.05597246983705792 0.37996728059528206 9 0.04560719764501016 0.34830334054567524 >10 0.18450184501845018 2.8462363889035482 >50 0.010365272192047763 0.5312505497211814 >100 0.016584435507276424 2.876141221172621 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CATGATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 336 0.5910602142593276 No Hit CATGATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 264 0.4644044540609003 No Hit CATGATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 264 0.4644044540609003 No Hit CATGATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 204 0.3588579872288775 No Hit CATGATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG 163 0.2867345682269953 No Hit CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 161 0.2832163526659278 No Hit CATGATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAACGATTAAG 132 0.23220222703045015 No Hit CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 111 0.19526096363924217 No Hit CATGATGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC 71 0.12489665241789363 No Hit CATGATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTCAG 67 0.1178602212957588 No Hit CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTCCTCTTCC 57 0.10026914349042165 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0035182155610674268 0.0 0.0 0.0 0.0 11 0.00527732334160114 0.0 0.0 0.0 0.0 12 0.0070364311221348536 0.0 0.0 0.0 0.0 13 0.0070364311221348536 0.0 0.0 0.0 0.0 14 0.01055464668320228 0.0 0.0 0.0 0.0 15 0.01055464668320228 0.0 0.0 0.0 0.0 16 0.012313754463735994 0.0 0.0 0.0 0.0 17 0.014072862244269707 0.0 0.0 0.0 0.0 18 0.019350185585870847 0.0 0.0 0.0 0.0 19 0.019350185585870847 0.0 0.0 0.0 0.0 20 0.02110929336640456 0.0 0.0 0.0 0.0 21 0.024627508927471987 0.0 0.0 0.0 0.0 22 0.0263866167080057 0.0 0.0 0.0 0.0 23 0.0263866167080057 0.0 0.0 0.0 0.0 24 0.029904832269073128 0.0 0.0 0.0 0.0 25 0.029904832269073128 0.0 0.0 0.0 0.0 26 0.03342304783014055 0.0 0.0 0.0 0.0 27 0.03518215561067427 0.0 0.0 0.0 0.0 28 0.03518215561067427 0.0 0.0 0.0 0.0 29 0.03518215561067427 0.0 0.0 0.0 0.0 30 0.03694126339120798 0.0 0.0 0.0 0.0 31 0.038700371171741695 0.0 0.0 0.0 0.0 32 0.038700371171741695 0.0 0.0 0.0 0.0 33 0.038700371171741695 0.0 0.0 0.0 0.0 34 0.038700371171741695 0.0 0.0 0.0 0.0 35 0.038700371171741695 0.0 0.0 0.0 0.0017591077805337134 36 0.038700371171741695 0.0 0.0 0.0 0.0017591077805337134 37 0.038700371171741695 0.0 0.0 0.0 0.0017591077805337134 38 0.038700371171741695 0.0 0.0 0.0 0.0017591077805337134 39 0.038700371171741695 0.0 0.0 0.0 0.0017591077805337134 40 0.038700371171741695 0.0 0.0 0.0 0.0017591077805337134 41 0.038700371171741695 0.0 0.0 0.0 0.0017591077805337134 42 0.038700371171741695 0.0 0.0 0.0 0.0017591077805337134 43 0.038700371171741695 0.0 0.0 0.0 0.0017591077805337134 44 0.038700371171741695 0.0 0.0 0.0 0.0017591077805337134 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACATCA 15 0.008029229 50.55814 41 ACCACTG 15 0.008029229 50.55814 39 AACCTTA 15 0.008029229 50.55814 41 TACGCCC 15 0.008029229 50.55814 38 TGCCCGT 35 4.8035872E-8 50.467857 37 GTACGCC 25 1.951375E-5 50.467857 37 TGCGTGA 15 0.008143531 50.377895 44 ATGCCCG 30 9.778723E-7 50.377895 36 ATGCTCG 15 0.008143531 50.377895 36 GCCGGCG 15 0.008172295 50.333035 42 GCGTTCA 15 0.008172295 50.333035 42 TATGCTT 160 0.0 50.288254 35 CGTTCAC 15 0.008230049 50.243557 43 CCAACAC 30 9.96095E-7 50.243557 43 TAAGAGA 15 0.008230049 50.243557 34 ATATGCT 180 0.0 50.243557 34 AATACAG 15 0.008230049 50.243557 34 CCTCAAA 25 2.003592E-5 50.243553 43 ATATGCG 20 4.0514738E-4 50.243553 34 TCCACGA 20 4.0871988E-4 50.154392 45 >>END_MODULE