FastQCFastQC Report
Fri 17 Jun 2016
SRR1526329_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526329_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12952
Sequences flagged as poor quality0
Sequence length56
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC230.1775787523162446No Hit
GGCTGCGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCCGTCGACCGTGCGCTT150.11581222977146387No Hit
GGCTGCGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCCTGCGCTT140.10809141445336627No Hit
GGCTGCGGGGATGAGATGGACTTAAAGGAATTGACGGGTCCGCACAGTTCTGAGTC140.10809141445336627No Hit
GGCTGCGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT130.10037059913526869No Hit
GGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC130.10037059913526869No Hit
GGCTGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC130.10037059913526869No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGCCT150.00806119950.28404641
AGACCCT150.00806119950.28404640
CGGAGAC251.9084033E-550.28404237
GGTTGGG203.9954894E-450.0891512
CCAAAAC203.9954894E-450.0891543
GGACTCT150.0081866350.089159
GAAGCTG150.0081866350.089159
GAAGCCT203.9954894E-450.089159
TCTATTT150.0081866350.0891546
TATTTTA150.0081866350.0891543
TCTGAGT150.0081866350.0891549
AGATGGT150.0081866350.0891511
TTCTATT150.0081866350.0891545
GTTGGGG203.9954894E-450.0891513
AAGCCTG203.9954894E-450.0891510
AGCTGGT150.0081866350.0891511
TTGCCTA150.0081866350.0891542
GGTCGAG150.0081866350.0891513
GTAGAGC204.0730918E-449.89575633
TGGTAGA204.0730918E-449.89575631