##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526329_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12952 Sequences flagged as poor quality 0 Sequence length 56 %GC 56 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.902177269919704 39.0 38.0 39.0 35.0 40.0 2 37.653721432983325 39.0 38.0 39.0 35.0 40.0 3 37.617279184681905 39.0 38.0 39.0 35.0 40.0 4 37.63063619518221 39.0 38.0 39.0 35.0 40.0 5 37.43715256331068 39.0 38.0 39.0 35.0 40.0 6 37.119904261890056 38.0 37.0 39.0 33.0 40.0 7 36.63411056207536 38.0 36.0 39.0 33.0 39.0 8 36.64159975293391 38.0 36.0 39.0 33.0 40.0 9 36.61504014823966 38.0 36.0 39.0 32.0 40.0 10 36.3733786287832 38.0 35.0 39.0 32.0 40.0 11 34.10060222359481 36.0 31.0 39.0 26.0 39.0 12 32.889592340951204 36.0 31.0 39.0 19.0 39.0 13 32.45243977764052 36.0 30.0 39.0 17.0 39.0 14 32.07388820259419 36.0 29.0 38.0 16.0 39.0 15 32.59349907350216 36.0 30.0 38.0 18.0 39.0 16 32.37229771463866 36.0 30.0 38.0 18.0 39.0 17 32.44186226065472 36.0 30.0 38.0 19.0 39.0 18 32.47467572575664 36.0 30.0 38.0 20.0 39.0 19 33.36596664607782 36.0 31.0 38.0 24.0 39.0 20 33.50586781964176 36.0 31.0 38.0 24.0 39.0 21 34.74374613959234 38.0 33.0 39.0 27.0 39.0 22 34.28119209388512 38.0 33.0 39.0 25.0 39.0 23 34.51412909203212 38.0 33.0 39.0 26.0 39.0 24 34.14075046324892 38.0 33.0 39.0 24.0 39.0 25 34.327825818406424 38.0 33.0 39.0 26.0 39.0 26 34.59488882025942 38.0 34.0 39.0 26.0 39.0 27 34.42804200123533 38.0 33.0 39.0 26.0 39.0 28 34.37909203211859 38.0 33.0 39.0 26.0 39.0 29 34.21903953057443 38.0 33.0 39.0 25.0 39.0 30 34.07991043854231 38.0 33.0 39.0 25.0 39.0 31 33.87330142063002 37.0 33.0 39.0 24.0 39.0 32 32.78883570105003 36.0 31.0 38.0 18.0 39.0 33 34.11789684990735 38.0 33.0 39.0 25.0 39.0 34 33.788295243977764 37.0 33.0 39.0 24.0 39.0 35 33.805203829524395 37.0 33.0 39.0 25.0 39.0 36 31.840642371834466 35.0 31.0 38.0 20.0 39.0 37 31.62083075972823 35.0 30.0 38.0 19.0 39.0 38 30.91846819024089 35.0 30.0 38.0 14.0 39.0 39 28.562615812229772 33.0 25.0 37.0 5.0 38.0 40 24.802733168622606 30.0 11.0 36.0 4.0 38.0 41 21.45097282273008 25.0 8.0 33.0 4.0 37.0 42 25.399166151945646 30.0 20.0 34.0 4.0 37.0 43 21.602532427424336 26.0 8.0 33.0 4.0 36.0 44 22.513357010500307 27.0 9.0 33.0 4.0 36.0 45 24.827825818406424 30.0 18.0 33.0 4.0 37.0 46 24.783662754786906 30.0 11.0 35.0 4.0 38.0 47 24.14615503397159 29.0 7.0 35.0 4.0 38.0 48 24.05064854848672 29.0 8.0 35.0 4.0 38.0 49 21.4399320568252 25.0 4.0 35.0 4.0 38.0 50 23.75216182828907 29.0 4.0 35.0 4.0 38.0 51 25.26343421865349 32.0 4.0 37.0 4.0 39.0 52 25.0633106856084 32.0 4.0 37.0 4.0 39.0 53 24.53698270537369 31.0 4.0 37.0 4.0 39.0 54 23.711781964175415 30.0 4.0 36.0 4.0 39.0 55 23.83153180975911 31.0 4.0 36.0 4.0 39.0 56 22.85423100679432 29.0 4.0 37.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.5691691942746715 1 2 0.8310493471455018 1 3 0.9312319912271647 1 4 -0.10940352228180927 1 5 0.527269528537893 1 6 1.7240165932135696 1 7 2.6312981223855942 1 8 2.735484344481044 1 9 2.894307317424577 1 10 1.4933859056749768 1 11 -5.194020273099213 1 12 -8.302034551168635 1 13 -20.495844410434188 1 14 -18.29908830902928 1 15 -11.937611733202868 1 16 -20.502095900244115 1 17 -21.46960175549281 1 18 -18.923456521870804 1 19 -4.641533877593268 1 20 -10.794429637449184 1 21 -8.601423275368713 1 22 -3.4064380684746496 1 23 -4.046706563273911 1 24 -3.2590209461590938 1 25 -4.15403957003609 1 26 2.440144909520889 1 27 3.5648177201911793 1 28 2.407538132876759 1 29 2.790976382864919 1 30 4.082198279518323 1 31 0.8470469569202166 1 32 -14.43226298182956 1 33 1.3825209657688404 1 34 -0.7657695303564154 1 35 1.6684338343258531 1 36 3.840659934392505 1 37 4.1857207485496986 1 38 6.00368617647543 1 39 7.719942208204593 1 40 3.9874330053403177 1 41 -13.445570100121193 1 42 -15.359088871869442 1 43 -15.38225309079894 1 44 -16.28295969288423 1 45 -18.657150244263246 1 46 -18.495371726513365 1 47 -17.63660800571405 1 48 -17.759060736591707 1 49 -14.979550619014635 1 50 -17.41009694120667 1 51 -18.952284322896748 1 52 -18.479745836951718 1 53 -18.06903614019423 1 54 -17.179468275496735 1 55 -17.569889111433223 1 56 -16.725225250827044 2 1 0.4489768865823649 2 2 0.3358570394531952 2 3 0.6379627604579312 2 4 -0.3545958299741159 2 5 1.0224618362301996 2 6 0.791324285521263 2 7 1.9486058146932876 2 8 1.9614458829425843 2 9 2.557768855886117 2 10 1.1376166749057433 2 11 -4.011327965406906 2 12 -13.191457628091712 2 13 -16.06315210274188 2 14 -16.034665232106203 2 15 -6.202034810125944 2 16 -5.155942054090268 2 17 -2.4455632939543506 2 18 -11.83211036802465 2 19 -2.386726185285575 2 20 -4.395391175910724 2 21 3.292807493862057 2 22 2.4349080853715037 2 23 3.4436780521107053 2 24 2.1255944384562895 2 25 3.163268122271603 2 26 3.493029524905502 2 27 0.6128946432680991 2 28 -0.5251541748155475 2 29 0.9832840751726124 2 30 2.7745059718260165 2 31 -1.3789145815413235 2 32 -4.509186058752636 2 33 -0.747286726538853 2 34 -1.0782695303564154 2 35 -0.3411815502895337 2 36 2.773352242084812 2 37 2.791489979318932 2 38 3.9219554072446634 2 39 7.489172977435359 2 40 8.973009928417241 2 41 -2.657108561659655 2 42 -4.339858102638672 2 43 -11.19475309079894 2 44 -11.90795969288423 2 45 -9.532150244263246 2 46 -13.870371726513365 2 47 -13.011608005714049 2 48 -12.384060736591707 2 49 -13.854550619014635 2 50 -11.597596941206671 2 51 -12.389784322896748 2 52 -11.729745836951718 2 53 -14.756536140194228 2 54 -15.866968275496735 2 55 -14.194889111433223 2 56 -13.475225250827044 3 1 0.5342041593096383 3 2 -0.24937023327407815 3 3 0.15500821500338446 3 4 0.40108598820770425 3 5 0.38041638168474634 3 6 0.916324285521263 3 7 1.6417876328751078 3 8 1.7739458829425843 3 9 2.5236779467952104 3 10 0.5807984930875634 3 11 -1.6136006926796327 3 12 0.03013328099919832 3 13 -7.779061193650971 3 14 -9.085801595742566 3 15 -3.7872620828532177 3 16 1.0258761277279156 3 17 1.219209433318376 3 18 1.594025995611716 3 19 -0.7674080034673914 3 20 -0.46925481227436094 3 21 2.7132620393166036 3 22 3.6564989944624102 3 23 3.432314415747072 3 24 2.216503529365383 3 25 3.856449940453423 3 26 2.055529524905502 3 27 2.2606219159953724 3 28 2.190754916093546 3 29 3.4378295297180657 3 30 2.2745059718260165 3 31 3.9904036002768564 3 32 1.3544503048837306 3 33 3.372031455279327 3 34 3.2342304696435846 3 35 3.1020002678922864 3 36 4.921079514812085 3 37 4.598308161137112 3 38 4.5526372254264835 3 39 7.852809341071726 3 40 10.546873564780874 3 41 9.274709620158529 3 42 7.5465055337249645 3 43 0.9359287273828762 3 44 4.535222125297587 3 45 8.706486119373118 3 46 -2.4499171810588187 3 47 -6.772971642077685 3 48 -1.94087891840989 3 49 -10.070459709923727 3 50 -1.6828242139339444 3 51 -1.7250115956240215 3 52 -1.5252003824062648 3 53 -1.2508543220124118 3 54 -5.861286457314916 3 55 -4.61534365688777 3 56 -2.861588887190681 4 1 0.7614768865823649 4 2 0.8150237061198595 4 3 0.9504627604579312 4 4 0.8745708366925484 4 5 0.3349618362301996 4 6 1.166324285521263 4 7 1.9486058146932876 4 8 2.1906125496092486 4 9 2.7661021892194526 4 10 0.6792833415724076 4 11 3.0303387012597582 4 12 2.7877090385749526 4 13 3.8118478972581187 4 14 4.027834767893797 4 15 6.318798523207391 4 16 6.344057945909732 4 17 4.950270039378985 4 18 6.355389631975349 4 19 3.675773814714425 4 20 4.33377549075594 4 21 2.167807493862057 4 22 2.164074752038168 4 23 1.4853447187773696 4 24 2.2505944384562895 4 25 2.288268122271603 4 26 2.638862858238838 4 27 2.2795613099347634 4 28 1.7665124918511168 4 29 2.066617408505948 4 30 1.2328393051593523 4 31 2.766918751792012 4 32 4.1991472745806995 4 33 2.7318799401278113 4 34 2.4842304696435846 4 35 2.0754851163771306 4 36 2.898352242084812 4 37 1.416489979318932 4 38 1.5261220739113313 4 39 5.239172977435359 4 40 7.827176595083909 4 41 4.009558105007013 4 42 7.201808564027996 4 43 7.6594135758677275 4 44 10.133706973782438 4 45 7.426183089070086 4 46 7.671294940153302 4 47 7.5717253276192835 4 48 8.449272596741626 4 49 2.645449380985365 4 50 7.506569725459997 4 51 4.422715677103252 4 52 5.93692082971495 4 53 4.2226305264724395 4 54 0.3205317245032653 4 55 3.5967775552334444 4 56 5.0247747491729555 5 1 0.2614768865823649 5 2 -0.05559032896785965 5 3 0.22677854993161617 5 4 -0.5749905668162185 5 5 0.5191723625459872 5 6 0.5742190223633656 5 7 1.2709742357459177 5 8 0.6423669355741666 5 9 1.853821487465062 5 10 -0.6847517461468868 5 11 3.2189351924878338 5 12 3.673673950855658 5 13 5.285532107784434 5 14 3.356782136314852 5 15 4.669675716189843 5 16 5.97563689327815 5 17 5.518252495519334 5 18 5.388284368817452 5 19 4.208668551556528 5 20 4.088161455668221 5 21 1.8520180201778444 5 22 2.115829138003086 5 23 0.726572788952808 5 24 2.6519102279299744 5 25 1.2224786485873906 5 26 2.108161103852872 5 27 0.5931578011628389 5 28 1.5208984567633976 5 29 1.11486302254103 5 30 1.3008217612997015 5 31 2.940164365827094 5 32 4.017129730721049 5 33 1.9533711681979895 5 34 0.39212520648568727 5 35 0.7048710812894114 5 36 2.043089084190072 5 37 3.995437347739987 5 38 2.8134027756657183 5 39 5.910225609014304 5 40 5.173667823154084 5 41 1.461312490971924 5 42 7.936457686835013 5 43 10.354589014464217 5 44 8.611777149221034 5 45 6.395481334684124 5 46 9.504628273486635 5 47 9.521286731128058 5 48 8.819886631829345 5 49 10.283607275722208 5 50 9.22148200616175 5 51 7.889820940261146 5 52 7.836043636732491 5 53 8.088858596647878 5 54 7.662636987661159 5 55 8.956426678040462 5 56 8.432669486015062 6 1 0.6705677956732714 6 2 0.7960843121804686 6 3 1.382280942276111 6 4 0.5829041700258841 6 5 0.607689108957473 6 6 2.0072333764303565 6 7 2.6417876328751078 6 8 1.7284913374880375 6 9 3.06913249224975 6 10 2.30807122036029 6 11 4.0227629436840004 6 12 6.393769644635562 6 13 6.720938806349025 6 14 5.823289313348344 6 15 7.303647008055876 6 16 6.844057945909732 6 17 6.764663978772923 6 18 4.866753268338982 6 19 6.096228360168972 6 20 4.985290642271096 6 21 3.80417113022569 6 22 4.065589903553324 6 23 3.750496233928885 6 24 4.307412620274469 6 25 1.1291772131806965 6 26 2.3282567976327755 6 27 2.2151673705408257 6 28 0.8725730979117259 6 29 3.6651022569907923 6 30 3.36541506273511 6 31 4.126767236640497 6 32 5.5362684867019105 6 33 4.46294054618842 6 34 4.0978668332799515 6 35 3.1020002678922864 6 36 3.102897696630265 6 37 4.5073990702280256 6 38 3.64354631633557 6 39 6.261900250162633 6 40 2.6377826556899677 6 41 2.183800529249435 6 42 5.001050988270418 6 43 10.70865600011015 6 44 9.762494852570317 6 45 7.979213392100391 6 46 10.277355546213908 6 47 8.545210176104135 6 48 10.513666536135567 6 49 9.884085744621729 6 50 8.771721240611512 6 51 7.547715677103252 6 52 8.929345072139192 6 53 8.476418405260318 6 54 10.502349906321449 6 55 8.20283816129405 6 56 6.592956567354772 7 1 0.5114768865823649 7 2 1.223357039453198 7 3 1.0504627604579326 7 4 0.9329041700258855 7 5 1.5849618362301996 7 6 0.21632428552126015 7 7 2.623605814693285 7 8 2.973945882942587 7 9 2.732768855886114 7 10 1.962616674905746 7 11 4.313672034593097 7 12 4.171042371908285 7 13 4.711847897258117 7 14 5.027834767893797 7 15 3.7354651898740556 7 16 5.894057945909729 7 17 5.241936706045649 7 18 4.680389631975352 7 19 3.050773814714425 7 20 3.3671088240892786 7 21 3.3178074938620554 7 22 3.3974080853715023 7 23 2.1186780521107025 7 24 3.1755944384562866 7 25 2.6882681222716016 7 26 2.7055295249055007 7 27 1.437894643268102 7 28 1.0498458251844554 7 29 0.8332840751726138 7 30 1.5245059718260165 7 31 2.8585854184586736 7 32 2.540813941247361 7 33 0.19021327346114703 7 34 0.7842304696435818 7 35 2.233818449710469 7 36 1.5983522420848146 7 37 1.8164899793189306 7 38 1.634455407244662 7 39 4.989172977435359 7 40 -2.9394900715827603 7 41 -9.157108561659655 7 42 4.910141897361328 7 43 8.61774690920106 7 44 7.017040307115771 7 45 5.342849755736754 7 46 9.204628273486634 7 47 8.213391994285953 7 48 8.490939263408293 7 49 9.720449380985364 7 50 8.539903058793328 7 51 10.147715677103253 7 52 10.970254163048285 7 53 7.880963859805771 7 54 9.820531724503265 7 55 7.680110888566777 7 56 6.224774749172955 8 1 1.213857838963314 8 2 1.5709760870722462 8 3 1.938557998553172 8 4 1.773380360502074 8 5 1.8349618362301996 8 6 2.463943333140314 8 7 2.799796290883762 8 8 2.8929935019902047 8 9 3.432768855886117 8 10 2.643569055858123 8 11 4.637481558402619 8 12 7.4305661814320985 8 13 6.954705040115265 8 14 7.492120482179512 8 15 6.890227094635961 8 16 8.296438898290681 8 17 6.753841467950409 8 18 6.65896106054678 8 19 6.003154767095374 8 20 5.429013585994035 8 21 4.072569398623962 8 22 4.223598561561978 8 23 4.04486852830118 8 24 3.839880152742005 8 25 4.776363360366844 8 26 4.007910477286451 8 27 3.1069422623157195 8 28 2.2427029680415984 8 29 4.007093598982138 8 30 3.488791686111732 8 31 2.737156847030107 8 32 3.300337750771174 8 33 1.4521180353659062 8 34 2.043754279167395 8 35 2.3314374973295173 8 36 0.05311414684671689 8 37 0.511728074557027 8 38 1.8535030262922838 8 39 4.584411072673454 8 40 -5.994251976344664 8 41 -11.669013323564418 8 42 1.3625228497422803 8 43 8.379651671105822 8 44 6.383706973782438 8 45 3.7714211843081813 8 46 9.21891398777235 8 47 9.411011041904999 8 48 10.145701168170199 8 49 10.829973190509175 8 50 9.018474487364756 8 51 8.047715677103252 8 52 9.139301782095902 8 53 8.550011478853392 8 54 9.582436486408028 8 55 7.239634698090587 8 56 9.131917606315813 9 1 0.7614768865823649 9 2 0.9324479485441017 9 3 0.33682639682156434 9 4 1.0374496245713374 9 5 0.47132547259383273 9 6 1.416324285521263 9 7 2.732696723784194 9 8 2.3648549738516778 9 9 2.5236779467952104 9 10 1.5353439476330166 9 11 3.8863993073203673 9 12 5.530133280999195 9 13 6.220938806349025 9 14 5.459652949711977 9 15 6.8491015535104225 9 16 7.571330673182459 9 17 6.264663978772923 9 18 6.548571450157169 9 19 5.368955632896245 9 20 4.894381551362002 9 21 3.0768984029529634 9 22 3.7474080853715037 9 23 3.932314415747072 9 24 1.9437762566381096 9 25 2.6291772131806965 9 26 3.1918931612691352 9 27 3.2606219159953724 9 28 2.827118552457179 9 29 2.6196477115362455 9 30 2.683596880916923 9 31 1.9449490548223096 9 32 3.0817230321564573 9 33 2.4174860007338737 9 34 2.0978668332799515 9 35 1.6474548133468332 9 36 0.46653406026662836 9 37 -0.6289645661356147 9 38 2.77990995269921 9 39 4.352809341071723 9 40 -2.453126435219122 9 41 -0.9980176525687448 9 42 4.228323715543144 9 43 9.345019636473786 9 44 7.853403943479407 9 45 6.161031573918571 9 46 9.277355546213908 9 47 10.090664721558678 9 48 10.150030172499203 9 49 11.565903926439912 9 50 7.953539422429692 9 51 9.365897495285072 9 52 10.111163253957375 9 53 9.476418405260318 9 54 8.956895360866902 9 55 10.70283816129405 9 56 7.956592930991139 10 1 1.061476886582362 10 2 0.12335703945319665 10 3 0.7504627604579284 10 4 0.5829041700258841 10 5 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12 -1.712290961425044 120 13 -4.854818769408549 120 14 -2.888831898772871 120 15 -6.014534810125944 120 16 -6.322608720756936 120 17 -8.452507738398793 120 18 -0.8807214791357616 120 19 0.050773814714425214 120 20 -3.6662245092440564 120 21 0.056696382750942576 120 22 1.6362969742603894 120 23 1.2353447187773696 120 24 -2.7632944504326 120 25 0.3160459000493816 120 26 1.8333073026832807 120 27 2.487894643268099 120 28 3.0998458251844525 120 29 2.4832840751726124 120 30 -1.003271805951762 120 31 -3.1358590259857664 120 32 -11.175852725419304 120 33 -2.643120059872185 120 34 -5.432436197023083 120 35 -2.605070439178423 120 36 -1.518314424581856 120 37 -0.027954465125510808 120 38 0.7900109628002205 120 39 2.0447285329909164 120 40 -4.8672678493605375 120 41 0.6484469938959023 120 42 -1.8120803248608937 120 43 4.61774690920106 120 44 -2.949626359550898 120 45 -13.768261355374356 120 46 -15.93981617095781 120 47 -15.85883022793627 120 48 -15.981282958813928 120 49 -14.868439507903524 120 50 -14.854541385651116 120 51 -16.841173211785637 120 52 -16.257523614729497 120 53 -16.18014725130534 120 54 -15.290579386607845 120 55 -15.681000222544334 120 56 -13.725225250827044 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 29.0 5 12.0 6 22.0 7 12.0 8 29.0 9 46.0 10 29.0 11 26.0 12 30.0 13 19.0 14 24.0 15 41.0 16 75.0 17 78.0 18 112.0 19 151.0 20 190.0 21 198.0 22 264.0 23 359.0 24 364.0 25 403.0 26 476.0 27 479.0 28 510.0 29 509.0 30 588.0 31 678.0 32 823.0 33 988.0 34 1150.0 35 1353.0 36 1359.0 37 975.0 38 440.0 39 111.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 100.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 98.6951822112415 0.8647313156269302 0.2779493514515133 0.1621371216800494 8 99.16615194564547 0.5713403335392218 0.16985793699814702 0.0926497838171711 9 97.44362063639173 1.5214704973741118 0.5174544331170837 0.5174544331170837 10 55.91414453366276 28.00339715873996 6.539530574428659 9.542927733168623 11 29.671093267449045 26.55960469425571 19.317479925880175 24.45182211241507 12 31.470043236565783 12.198888202594194 27.20043236565781 29.130636195182213 13 32.26528721432984 13.804817788758491 23.91908585546634 30.01080914144534 14 27.92368299088522 15.309748184767496 24.617642515062567 32.14892630928472 15 24.435508815341787 14.406124342715742 26.283637488400867 34.8747293535416 16 27.83099026726402 17.480302796230497 29.07461764251506 25.61408929399042 17 32.37065637065637 20.06949806949807 21.135135135135137 26.424710424710423 18 30.661724963323294 22.006022700949735 19.25720021619952 28.07505211952745 19 35.7402115993513 22.233377094756353 18.580585373387905 23.44582593250444 20 31.559845559845563 24.26254826254826 19.305019305019304 24.872586872586872 21 30.337425681414565 19.813141842328776 24.384217434947107 25.465215041309552 22 28.667387276096356 20.99289684990735 25.617665225447805 24.72205064854849 23 29.439468807906117 23.35546633724521 24.683446571957997 22.521618282890675 24 26.613650401482396 23.424953675108092 23.162445954292775 26.798949969116737 25 25.579061148857317 24.544471896232242 24.467263743051266 25.40920321185917 26 25.315182922113078 23.590378219506537 25.09861551550777 25.995823342872608 27 26.03490886623417 18.759654000617857 27.741736175471114 27.463700957676863 28 24.66929682060803 21.025760037131587 28.83112864547072 25.473814496789664 29 25.09267840593142 22.31232622798888 27.79579857893111 24.799196787148595 30 24.013284930872018 22.80837259596818 31.945624468988953 21.23271800417085 31 24.43515939337666 21.13122872175797 32.814917982048904 21.618693902816467 32 26.866939532010193 18.271681210904315 32.759286431384666 22.102092825700826 33 27.94493000232037 17.959625647768583 33.04973315801686 21.045711191894192 34 23.1643221518009 23.458030607512754 35.56963982068326 17.80800742000309 35 22.568183574132735 22.04280305956888 35.957660511473385 19.431352854825 36 24.945870708320445 21.574389112279615 34.83606557377049 18.643674605629446 37 23.879210220673635 22.493224932249323 33.31784746418893 20.309717382888117 38 25.740354132838476 22.655223072759608 33.92870950282224 17.67571329157968 39 22.561400790268845 18.45510188269931 32.64120244828388 26.342294878747968 40 13.72533789032158 15.441976075811715 24.654342084822122 46.17834394904459 41 15.545695158025843 16.970263116923554 27.728475790129224 39.75556593492138 42 20.800123810260775 23.22990017797725 35.37104387526116 20.598932136500814 43 11.819800294140412 13.499496865082437 28.856722656552364 45.82398018422478 44 15.018229772709644 14.847568070747034 26.607710805988678 43.52649135055465 45 19.863966609986086 20.265883444118103 36.11068171278405 23.759468233111765 46 18.782353395657886 23.588039867109632 37.356099822297764 20.273506914934714 47 20.515790286464366 18.392402131109566 35.49532854605822 25.596479036367846 48 20.435588507877664 21.69447018844609 39.49644732777263 18.373493975903614 49 15.626451912652936 14.704971348923648 26.901037633575964 42.76753910484745 50 18.313346228239848 20.93617021276596 38.46808510638298 22.28239845261122 51 19.71961892959492 23.119820308264273 37.70428316939044 19.456277592750368 52 19.576842594745408 24.071921258622027 35.433620088351546 20.91761605828102 53 20.820116054158607 24.851063829787236 35.26499032882012 19.06382978723404 54 18.935002705000386 20.75121725017389 33.52654764664966 26.78723239817606 55 20.595514307811293 22.53673627223511 37.115235885537516 19.752513534416085 56 19.594646863154637 23.013846986926588 34.68708903844666 22.704417111472114 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.5 13 1.0 14 1.0 15 6.0 16 11.0 17 16.5 18 22.0 19 25.5 20 29.0 21 34.0 22 47.0 23 55.0 24 68.5 25 82.0 26 96.5 27 111.0 28 135.0 29 207.0 30 255.0 31 282.5 32 310.0 33 357.0 34 404.0 35 416.5 36 429.0 37 412.5 38 386.0 39 376.0 40 324.5 41 273.0 42 249.0 43 225.0 44 214.5 45 204.0 46 206.5 47 221.0 48 233.0 49 245.0 50 257.0 51 297.0 52 337.0 53 366.5 54 431.0 55 466.0 56 471.0 57 476.0 58 538.0 59 600.0 60 599.0 61 598.0 62 638.5 63 765.5 64 852.0 65 882.0 66 912.0 67 887.5 68 863.0 69 825.5 70 788.0 71 696.0 72 531.5 73 459.0 74 401.0 75 343.0 76 284.5 77 226.0 78 179.5 79 104.5 80 76.0 81 57.0 82 38.0 83 25.0 84 12.0 85 10.5 86 9.0 87 6.0 88 1.5 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.030883261272390366 10 0.0 11 0.0 12 0.0 13 0.0 14 0.04632489190858555 15 0.1544163063619518 16 0.04632489190858555 17 0.015441630636195183 18 0.0077208153180975915 19 0.023162445954292774 20 0.015441630636195183 21 0.0077208153180975915 22 0.0 23 0.0 24 0.0 25 0.0 26 0.1775787523162446 27 0.030883261272390366 28 0.19302038295243978 29 0.030883261272390366 30 0.03860407659048795 31 0.21618282890673254 32 0.023162445954292774 33 0.1775787523162446 34 0.10809141445336627 35 0.06948733786287832 36 0.1544163063619518 37 0.2856701667696109 38 0.14669549104385424 39 0.3474366893143916 40 0.6022235948116121 41 0.8184064237183446 42 0.22390364422483017 43 0.2547869054972205 44 0.47096973440395307 45 0.10809141445336627 46 0.06948733786287832 47 0.0077208153180975915 48 0.030883261272390366 49 0.2933909820877085 50 0.20846201358863498 51 0.31655342804200126 52 0.378319950586782 53 0.20846201358863498 54 0.10037059913526869 55 0.16985793699814702 56 0.19302038295243978 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 12952.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.90858554663373 #Duplication Level Percentage of deduplicated Percentage of total 1 95.00168010752688 87.31470043236565 2 3.578629032258065 6.578134651019147 3 0.7476478494623656 2.0614576899320567 4 0.19321236559139784 0.7103150092649784 5 0.18481182795698925 0.8492896849907351 6 0.10080645161290322 0.5558987029030266 7 0.04200268817204301 0.2702285361334157 8 0.025201612903225805 0.1852995676343422 9 0.008400537634408603 0.06948733786287832 >10 0.11760752688172044 1.4051883878937617 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGCTGCGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC 23 0.1775787523162446 No Hit GGCTGCGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCCGTCGACCGTGCGCTT 15 0.11581222977146387 No Hit GGCTGCGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCCTGCGCTT 14 0.10809141445336627 No Hit GGCTGCGGGGATGAGATGGACTTAAAGGAATTGACGGGTCCGCACAGTTCTGAGTC 14 0.10809141445336627 No Hit GGCTGCGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT 13 0.10037059913526869 No Hit GGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 13 0.10037059913526869 No Hit GGCTGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC 13 0.10037059913526869 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0077208153180975915 0.0 0.0 0.0 0.0 12 0.0077208153180975915 0.0 0.0 0.0 0.0 13 0.023162445954292774 0.0 0.0 0.0 0.0 14 0.030883261272390366 0.0 0.0 0.0 0.0 15 0.030883261272390366 0.0 0.0 0.0 0.0 16 0.030883261272390366 0.0 0.0 0.0 0.0 17 0.030883261272390366 0.0 0.0 0.0 0.0 18 0.03860407659048796 0.0 0.0 0.0 0.0 19 0.054045707226683136 0.0 0.0 0.0 0.0 20 0.054045707226683136 0.0 0.0 0.0 0.0 21 0.054045707226683136 0.0 0.0 0.0 0.0 22 0.054045707226683136 0.0 0.0 0.0 0.0 23 0.06176652254478073 0.0 0.0 0.0 0.0 24 0.06176652254478073 0.0 0.0 0.0 0.0 25 0.06176652254478073 0.0 0.0 0.0 0.0 26 0.06176652254478073 0.0 0.0 0.0 0.0 27 0.06948733786287832 0.0 0.0 0.0 0.0 28 0.07720815318097592 0.0 0.0 0.0 0.0 29 0.07720815318097592 0.0 0.0 0.0 0.0 30 0.07720815318097592 0.0 0.0 0.0 0.0 31 0.07720815318097592 0.0 0.0 0.0 0.0 32 0.07720815318097592 0.0 0.0 0.0 0.0 33 0.07720815318097592 0.0 0.0 0.0 0.0 34 0.07720815318097592 0.0 0.0 0.0 0.0 35 0.07720815318097592 0.0 0.0 0.0 0.0 36 0.07720815318097592 0.0 0.0 0.0 0.0 37 0.07720815318097592 0.0 0.0 0.0 0.0 38 0.07720815318097592 0.0 0.0 0.0 0.0 39 0.07720815318097592 0.0 0.0 0.0 0.0 40 0.07720815318097592 0.0 0.0 0.0 0.0 41 0.0849289684990735 0.0 0.0 0.0 0.0 42 0.0926497838171711 0.0 0.0 0.0 0.0 43 0.10037059913526869 0.0 0.0 0.0 0.0 44 0.10037059913526869 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTGCCT 15 0.008061199 50.284046 41 AGACCCT 15 0.008061199 50.284046 40 CGGAGAC 25 1.9084033E-5 50.284042 37 GGTTGGG 20 3.9954894E-4 50.08915 12 CCAAAAC 20 3.9954894E-4 50.08915 43 GGACTCT 15 0.00818663 50.08915 9 GAAGCTG 15 0.00818663 50.08915 9 GAAGCCT 20 3.9954894E-4 50.08915 9 TCTATTT 15 0.00818663 50.08915 46 TATTTTA 15 0.00818663 50.08915 43 TCTGAGT 15 0.00818663 50.08915 49 AGATGGT 15 0.00818663 50.08915 11 TTCTATT 15 0.00818663 50.08915 45 GTTGGGG 20 3.9954894E-4 50.08915 13 AAGCCTG 20 3.9954894E-4 50.08915 10 AGCTGGT 15 0.00818663 50.08915 11 TTGCCTA 15 0.00818663 50.08915 42 GGTCGAG 15 0.00818663 50.08915 13 GTAGAGC 20 4.0730918E-4 49.895756 33 TGGTAGA 20 4.0730918E-4 49.895756 31 >>END_MODULE