FastQCFastQC Report
Fri 17 Jun 2016
SRR1526328_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526328_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64843
Sequences flagged as poor quality0
Sequence length56
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC2700.41639035824992676No Hit
TAGCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1690.2606295205342134No Hit
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG1070.16501395678793393No Hit
TAGCATGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC1050.1619295837638604No Hit
TAGCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTCCCTACCAAGCGC1010.15576083771571334No Hit
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG860.13262804003516185No Hit
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG750.11566398840275743No Hit
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG710.10949524235461038No Hit
TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG660.10178430979442654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCTT600.050.3062535
TATGCCC251.9919818E-550.3062535
GTAAGCC150.00819471750.3062535
ATACAAA204.0308275E-450.3062536
TACCCGT150.00822007950.2669849
CACGATT252.0012229E-550.2669847
CGATTAA309.950891E-750.2669849
ACGATTA309.950891E-750.2669848
AAGATTA600.050.2669848
TGTACAT150.00822007950.2669846
AAAGATT600.050.2669847
CATTTGA204.0464132E-450.26697546
AAGGACA150.00824550150.2277745
GTAACTT150.00824550150.2277745
CGACTCC252.0104995E-550.22776844
CCAAAAC1200.050.1886243
TGATACA204.1250617E-450.07153734
TGATATG204.156859E-449.99378650
TCTATTA204.156859E-449.99378650
ATCTCAT252.0764382E-549.95500612