##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526328_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 64843 Sequences flagged as poor quality 0 Sequence length 56 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.377450148821 39.0 38.0 39.0 33.0 40.0 2 35.7212806316796 39.0 36.0 39.0 28.0 40.0 3 36.6501087241491 39.0 36.0 39.0 31.0 40.0 4 36.550175038169115 39.0 36.0 39.0 30.0 40.0 5 35.26463920546551 39.0 35.0 39.0 21.0 40.0 6 36.78488040343599 38.0 37.0 39.0 32.0 40.0 7 36.8715050198171 38.0 37.0 39.0 32.0 40.0 8 36.59917030365652 38.0 36.0 39.0 31.0 40.0 9 36.53942599818022 38.0 36.0 39.0 31.0 40.0 10 36.314868220162545 38.0 36.0 39.0 31.0 40.0 11 35.24608670172571 38.0 34.0 39.0 28.0 40.0 12 33.22656262048332 37.0 31.0 39.0 19.0 40.0 13 32.585398578104034 36.0 30.0 39.0 17.0 40.0 14 32.152136699412424 36.0 29.0 39.0 16.0 40.0 15 32.71463380781272 36.0 30.0 39.0 18.0 40.0 16 32.509260830004784 36.0 30.0 38.0 18.0 40.0 17 32.53422882963466 36.0 30.0 38.0 19.0 40.0 18 32.629073300124915 36.0 30.0 38.0 20.0 40.0 19 33.52543836651605 36.0 31.0 38.0 24.0 40.0 20 33.81945622503586 36.0 31.0 38.0 25.0 40.0 21 35.332942646083616 38.0 35.0 39.0 28.0 40.0 22 35.04180867634132 38.0 35.0 39.0 27.0 40.0 23 35.29949262063754 38.0 35.0 39.0 28.0 40.0 24 34.856021467236246 38.0 34.0 39.0 26.0 40.0 25 35.12769304319664 38.0 35.0 39.0 27.0 40.0 26 35.47027435498049 38.0 35.0 39.0 29.0 40.0 27 35.45099702358003 38.0 35.0 39.0 29.0 40.0 28 35.41244236077911 38.0 35.0 39.0 29.0 40.0 29 35.24061193960797 38.0 35.0 39.0 28.0 40.0 30 35.12140092222754 38.0 35.0 39.0 28.0 40.0 31 34.820582021189644 38.0 35.0 39.0 27.0 40.0 32 33.76208688678809 38.0 33.0 39.0 20.0 40.0 33 35.108168961954256 38.0 35.0 39.0 28.0 40.0 34 34.858442700060145 38.0 35.0 39.0 27.0 40.0 35 34.90524806070046 38.0 35.0 39.0 28.0 40.0 36 32.827090048270435 36.0 32.0 38.0 23.0 39.0 37 32.644849868143055 36.0 31.0 38.0 23.0 39.0 38 31.802615548324415 35.0 31.0 38.0 17.0 39.0 39 29.89938775195472 35.0 28.0 38.0 8.0 39.0 40 25.652807550545162 31.0 13.0 38.0 4.0 39.0 41 22.30459725799238 26.0 10.0 33.0 4.0 37.0 42 26.097034375337355 30.0 22.0 35.0 4.0 37.0 43 22.248461668954242 27.0 9.0 33.0 4.0 37.0 44 23.31081226963589 28.0 10.0 33.0 4.0 37.0 45 25.854710608701016 30.0 22.0 35.0 4.0 38.0 46 25.897830143577565 31.0 18.0 36.0 4.0 38.0 47 25.168730626281942 30.0 12.0 36.0 4.0 38.0 48 25.04651234520303 30.0 13.0 36.0 4.0 38.0 49 22.55666764338479 27.0 5.0 35.0 4.0 38.0 50 24.92612926607344 30.0 13.0 36.0 4.0 38.0 51 26.443116450503524 33.0 10.0 38.0 4.0 39.0 52 26.272072544453525 33.0 4.0 38.0 4.0 39.0 53 25.84848017519239 32.0 4.0 38.0 4.0 39.0 54 24.934796354271086 32.0 4.0 38.0 4.0 39.0 55 25.139089801520594 32.0 4.0 38.0 4.0 39.0 56 23.819379115710255 30.0 4.0 37.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.3129476770517243 1 2 3.3163967475985814 1 3 2.1508426264022162 1 4 2.2539185740927437 1 5 3.9414977844013848 1 6 2.0061243610378483 1 7 2.294431341370057 1 8 2.51403456562511 1 9 2.4582767588332928 1 10 2.319923720331012 1 11 -4.406896194131001 1 12 -8.506178446872845 1 13 -21.861383620838918 1 14 -18.775217459688584 1 15 -11.390761815631137 1 16 -20.559697067648976 1 17 -21.13405997898574 1 18 -20.869527518594502 1 19 -5.717563529178971 1 20 -9.257707746012517 1 21 -7.186218672039708 1 22 -2.472647718992956 1 23 -3.3176566962850096 1 24 -3.6239973297059116 1 25 -3.3089492866752934 1 26 2.6512359994653494 1 27 2.668857315799386 1 28 2.277357025955645 1 29 1.8465074518388391 1 30 2.164112706176134 1 31 1.7991828133932728 1 32 -10.354708827292637 1 33 1.8982557549212942 1 34 2.1491611156960957 1 35 2.036276038630845 1 36 3.792964821438087 1 37 4.149710193259484 1 38 5.264298959758126 1 39 7.1557470188491905 1 40 9.60281492380825 1 41 -3.3886027014804263 1 42 -8.251786195537472 1 43 -14.723063838591292 1 44 -16.513156287294585 1 45 -16.725071756634982 1 46 -18.711310837359342 1 47 -18.3407015586433 1 48 -17.04121576757215 1 49 -15.323545961976553 1 50 -17.17879148050704 1 51 -18.3145934877142 1 52 -17.98434208145106 1 53 -17.928216341620672 1 54 -18.40023788925728 1 55 -17.890006835777346 1 56 -16.46381400402095 2 1 1.0735993061244073 2 2 3.0344418603805394 2 3 1.941569443444827 2 4 1.7332418823634157 2 5 3.9991418946770736 2 6 1.7736682206869716 2 7 2.006837356407651 2 8 2.2565157686326245 2 9 2.3072742525676304 2 10 1.676440011057835 2 11 -2.811658098892906 2 12 -10.639010527073346 2 13 -13.06376457321987 2 14 -16.755167334375297 2 15 -10.22284201613239 2 16 -3.805311102736699 2 17 -2.0920800291110524 2 18 -10.6038633581935 2 19 -2.1323504965975175 2 20 -3.930640076839584 2 21 1.6446083956294615 2 22 2.3995327321348654 2 23 1.491240546822759 2 24 1.8158522943542366 2 25 1.1979429439011469 2 26 1.735195899214716 2 27 1.829258318305648 2 28 1.745402138737603 2 29 2.217434770134574 2 30 1.9009548114392913 2 31 -0.6851530262057253 2 32 -10.539545920024466 2 33 -1.463899633549886 2 34 -0.9040970296673123 2 35 -0.7087114300408359 2 36 3.28732572369373 2 37 3.241815456417381 2 38 4.533095952239325 2 39 6.57993248250834 2 40 8.100935224560136 2 41 4.048740656915562 2 42 -0.6151947168407297 2 43 -4.942362084205326 2 44 -10.29824400659283 2 45 -0.08910684435427996 2 46 -10.88236346893829 2 47 -11.11012511754054 2 48 -8.7279325595521 2 49 -12.589210122377557 2 50 -7.879919300055912 2 51 -7.879129828566331 2 52 -8.329580176689156 2 53 -8.214557193750998 2 54 -15.434072475723445 2 55 -8.592387788158298 2 56 -8.012686184472079 3 1 1.367750947363426 3 2 3.0354820822945427 3 3 2.281144108262211 3 4 1.423949233264942 3 5 4.1244308452087495 3 6 2.0568397417225697 3 7 2.2953255672259587 3 8 2.444795935068136 3 9 2.536585394500129 3 10 2.312131180729587 3 11 1.6586377864515214 3 12 2.1452012158762592 3 13 -3.7327428441398887 3 14 -4.419060075937942 3 15 -3.011099066525361 3 16 2.324947796939675 3 17 2.1647392034363406 3 18 1.7087811170723342 3 19 0.48299855564474115 3 20 0.3294154016624944 3 21 2.7898927229526294 3 22 2.9837907071695398 3 23 2.774065327220356 3 24 3.4774334316635347 3 25 2.6384191343773367 3 26 2.205145043921142 3 27 2.1783105605617763 3 28 2.3945006130787974 3 29 1.8698850706431287 3 30 2.4956150056140487 3 31 2.7488506723610797 3 32 2.4751327669843164 3 33 2.418439709954505 3 34 2.9139797155939036 3 35 2.0015289768015165 3 36 3.3490455572582185 3 37 4.258805747679514 3 38 5.410696507024351 3 39 7.974985649406165 3 40 10.470214004033085 3 41 9.747191882065817 3 42 7.2819388938851155 3 43 2.954887106733185 3 44 3.7422090678408253 3 45 6.8421229291085055 3 46 1.0642384728092829 3 47 -3.727901354248818 3 48 0.8283550040170162 3 49 -9.433407995701643 3 50 0.7979586472395113 3 51 -0.3235357462732189 3 52 0.08119189913146485 3 53 -0.6884360656881228 3 54 -6.971520464627744 3 55 -1.8341815930589007 3 56 1.8142902371645384 4 1 1.7203505299784325 4 2 3.5147489364820075 4 3 2.2969321492748165 4 4 2.096392750187185 4 5 4.336480568464346 4 6 2.4169704013723248 4 7 2.654367396461062 4 8 2.949105888792843 4 9 2.993077545847555 4 10 2.167316735579419 4 11 3.6468421236260085 4 12 3.470580038124698 4 13 5.40263507074987 4 14 3.089514463577526 4 15 5.6854801912552375 4 16 6.186121919070153 4 17 6.352290242362024 4 18 5.277422801130044 4 19 4.714556490496385 4 20 4.398465397125705 4 21 3.0514842300753955 4 22 3.103471316914572 4 23 2.8685213656923665 4 24 3.468722788346227 4 25 2.956176143723134 4 26 2.8809457879552625 4 27 2.3437220477226504 4 28 2.2514101494185113 4 29 2.7996555088973736 4 30 2.4546931291963006 4 31 3.1540770583514615 4 32 4.02109048406988 4 33 2.3689886619552354 4 34 2.981305729567225 4 35 2.9019917297277473 4 36 4.77931504278585 4 37 5.061908914361307 4 38 6.001387006978977 4 39 8.405255134933796 4 40 10.712750978899255 4 41 5.493222187845692 4 42 8.287564517694207 4 43 8.08645411517162 4 44 10.961880603999067 4 45 8.36494299988248 4 46 8.449412231996288 4 47 8.666799671066489 4 48 7.984239225049592 4 49 4.681372877177406 4 50 8.85539445161298 4 51 7.272961849226281 4 52 7.667527077427444 4 53 6.680858916618384 4 54 2.073938205184433 4 55 6.303918397867513 4 56 6.794056138625386 5 1 2.0855040680291665 5 2 3.6926051256866614 5 3 2.51980073596183 5 4 2.5699765762409683 5 5 4.669209921887962 5 6 2.6444165200067005 5 7 2.996633274775 5 8 2.843250462510177 5 9 2.5691790144723896 5 10 2.2444672219421875 5 11 3.577797683420023 5 12 6.318472466123932 5 13 6.470249032222309 5 14 6.374084366304974 5 15 5.851987915840397 5 16 6.825641278215684 5 17 6.1868315355147985 5 18 6.731170655411944 5 19 5.371050863946699 5 20 4.8448701272420465 5 21 3.639506354813136 5 22 3.6495327321348654 5 23 3.2242337441016744 5 24 3.979117600476691 5 25 3.342500767030401 5 26 3.0379169876500924 5 27 2.9857209033396614 5 28 2.2147898938396438 5 29 2.9708361306787907 5 30 2.7427915461331693 5 31 3.292738130256865 5 32 4.390726188819073 5 33 2.774195604545355 5 34 3.2761750791762267 5 35 3.231764760435361 5 36 4.460795111448828 5 37 4.634672599274527 5 38 5.548402074688308 5 39 7.974490305637595 5 40 6.452976040886664 5 41 3.4790127657591015 5 42 8.605893718533423 5 43 12.882467847767462 5 44 11.654136945788121 5 45 8.878580230475656 5 46 10.250289592286197 5 47 10.75892250150708 5 48 10.783972202352661 5 49 11.427796680343533 5 50 10.053754169331842 5 51 9.591958606807822 5 52 10.30647424507955 5 53 10.52693940488846 5 54 9.739396912031658 5 55 9.390605409120614 5 56 8.257721978793228 6 1 2.0472645297896293 6 2 3.365971441910119 6 3 2.48630248817787 6 4 2.545651694773227 6 5 4.117468013003197 6 6 2.122153069171816 6 7 2.7153510649213644 6 8 3.071450833567688 6 9 3.0331039783973566 6 10 2.064607399225217 6 11 4.0451240078892 6 12 6.460195822133006 6 13 6.545182035726736 6 14 7.36460178539382 6 15 6.3774465841562105 6 16 6.646709099283505 6 17 6.30546686843585 6 18 6.033582529252392 6 19 4.687274323027303 6 20 4.295189648990146 6 21 3.0425160435371126 6 22 3.854438937041067 6 23 3.3184411240233374 6 24 4.001999480501425 6 25 3.173772669730873 6 26 2.3232189872378086 6 27 1.767209256256585 6 28 2.124911518246982 6 29 2.0771028798026876 6 30 2.8125709730554505 6 31 2.765424174371475 6 32 4.228491598013051 6 33 2.7244120547617996 6 34 3.0735364479661698 6 35 2.7757763044469 6 36 3.7021886385566454 6 37 3.5322194968214227 6 38 4.711667380810756 6 39 6.580653983229844 6 40 4.096966970591879 6 41 3.779260137435042 6 42 8.205151603505591 6 43 12.853092461249215 6 44 11.17145296310414 6 45 8.650792145544713 6 46 11.062080975506152 6 47 11.37075511333969 6 48 11.185126603507062 6 49 12.826374293206857 6 50 10.584366414229802 6 51 10.314593115156612 6 52 10.29596094885197 6 53 10.185154205960401 6 54 10.625812084161112 6 55 10.309488113717602 6 56 10.422018000232107 7 1 1.724032206557304 7 2 3.1437492196878978 7 3 2.405494407369787 7 4 1.9395910887126249 7 5 4.238680134215315 7 6 1.627203574222321 7 7 2.3719167214870183 7 8 2.4148851770020343 7 9 1.992699937993315 7 10 2.195920530538352 7 11 3.4592654220306187 7 12 5.409690771627954 7 13 5.858313348858051 7 14 6.80904622983827 7 15 6.205729412439041 7 16 5.293173745748149 7 17 5.749911312880293 7 18 5.5992390949089526 7 19 4.61656725232023 7 20 4.729533083333578 7 21 3.0930210940421645 7 22 2.7130247956269287 7 23 2.9851077906900017 7 24 3.527252005753951 7 25 2.7899342858924854 7 26 2.090895754914577 7 27 2.4641789532262877 7 28 2.124911518246982 7 29 2.743769546469352 7 30 2.7923689528534297 7 31 2.866434275381579 7 32 3.864855234376691 7 33 2.9870383173880626 7 34 2.871516245945962 7 35 2.725271253941848 7 36 1.9446128809808876 7 37 1.9160578806598032 7 38 3.4490411181844927 7 39 5.09580549838136 7 40 -0.6707097970848892 7 41 -10.018719660544754 7 42 6.407171805525792 7 43 10.812688420845177 7 44 9.545190336841515 7 45 5.903317398069962 7 46 8.183293096718273 7 47 8.56267430525888 7 48 9.144722563103024 7 49 10.482939949772515 7 50 9.52376035362374 7 51 9.839845640409138 7 52 9.932324585215603 7 53 10.31646733727353 7 54 11.0096504679995 7 55 10.04686185109134 7 56 9.624038202252308 8 1 2.014075496600597 8 2 3.2742377787478816 8 3 2.3769435931046914 8 4 2.0597724946083176 8 5 4.118189513724694 8 6 2.4097226424556766 8 7 2.690510825795407 8 8 2.567740258428543 8 9 2.5385667695744303 8 10 1.9587529362279028 8 11 3.679838499746552 8 12 5.298064302858624 8 13 6.2253510730386346 8 14 6.935308856100892 8 15 6.392804242371014 8 16 6.58074331903201 8 17 6.105198882453578 8 18 5.547497186024188 8 19 4.238397802722211 8 20 4.62038033132368 8 21 2.9864451303233395 8 22 3.1801449770328247 8 23 2.969131703285342 8 24 2.928097192313423 8 25 2.7812762772344826 8 26 1.7522027019358077 8 27 1.9857209033396614 8 28 1.5515245877171964 8 29 1.9708361306787907 8 30 2.304016035929088 8 31 1.9355952731140036 8 32 3.768277209227236 8 33 2.3252160127086157 8 34 2.12311385468643 8 35 1.588907617578215 8 36 0.2771216420610756 8 37 0.12446851764186917 8 38 1.2830959522393286 8 39 3.1173474484947334 8 40 -3.3123300815623153 8 41 -10.582211724036819 8 42 3.871199840982399 8 43 7.453896419196035 8 44 6.817402251910572 8 45 3.950008801904225 8 46 7.107432449429055 8 47 7.238514338241771 8 48 7.834992610515929 8 49 9.876776272180265 8 50 6.71701947545429 8 51 7.796040239460879 8 52 8.398310979773427 8 53 7.73102103754152 8 54 8.270009156929618 8 55 8.064074796875715 8 56 8.982211774711594 9 1 2.1295770163574375 9 2 3.4941683041495324 9 3 2.426878460668732 9 4 2.0235154385944227 9 5 4.227612006125909 9 6 2.0467178660770387 9 7 2.6390562013924566 9 8 2.7648744312466107 9 9 2.5828568260225424 9 10 2.1511107304093997 9 11 3.715444231400916 9 12 5.5405740727240165 9 13 6.686488719586617 9 14 6.967223749717913 9 15 6.3830343769780455 9 16 7.196461946908691 9 17 6.197686941506987 9 18 6.233775952850067 9 19 5.485683951224168 9 20 4.756290014345829 9 21 3.4204442618908644 9 22 3.9139704221044695 9 23 3.380551590389125 9 24 3.967827978244813 9 25 2.76607870884542 9 26 2.708346861727385 9 27 2.267961459136451 9 28 2.1998094335704295 9 29 2.406137910965377 9 30 2.565848428460569 9 31 2.8333373486676265 9 32 3.6060974437040088 9 33 2.760517792995202 9 34 2.363886759593072 9 35 1.8867799580037499 9 36 1.9757755717180423 9 37 1.8535175840769575 9 38 3.2116673808107556 9 39 4.17618374897237 9 40 0.13360999659660777 9 41 -3.45607190640764 9 42 6.698381777586828 9 43 10.0168577739507 9 44 9.368422660073836 9 45 6.263730035078346 9 46 9.872955679997883 9 47 10.90481915804203 9 48 10.242938767702203 9 49 13.057193119770364 9 50 11.148196201463843 9 51 10.07958344397673 9 52 9.967785578123404 9 53 9.615736625904525 9 54 10.122158527316067 9 55 10.21105699400989 9 56 10.427283420391145 10 1 2.0847360188740183 10 2 3.699956453314485 10 3 2.7626139902958258 10 4 2.6456842786767822 10 5 4.7564383616509645 10 6 2.262915532514924 10 7 2.730339660555117 10 8 3.026485046666423 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1.086027022395026 119 37 1.4009063655082876 119 38 2.5753037444471225 119 39 4.393785296361152 119 40 6.571714445339353 119 41 7.226229834404741 119 42 2.8187879671419545 119 43 -3.071149962993207 119 44 0.050240841892019716 119 45 -1.3315310867785222 119 46 -9.041454378029199 119 47 -10.260558017973443 119 48 -12.660832992452532 119 49 -7.749383282550717 119 50 -7.801997222133835 119 51 -8.29904324847975 119 52 -7.143432990541971 119 53 -9.923431652625457 119 54 -9.61913741078838 119 55 -10.571825017595527 119 56 -7.1815173533032475 120 1 0.7770625095876085 120 2 2.5831431590818354 120 3 1.5443832962586796 120 4 0.9345405836621197 120 5 3.4331245786597577 120 6 0.826698523717269 120 7 1.1496944992647968 120 8 1.0714508335676882 120 9 1.3159322612256403 120 10 0.5747084093262274 120 11 -12.611441648676454 120 12 -2.4009152889781085 120 13 -6.906838166293465 120 14 -6.382872962080924 120 15 -7.877603920894295 120 16 -10.729553526979121 120 17 -9.886452323483343 120 18 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56 -9.11333553512143 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 568.0 5 18.0 6 32.0 7 53.0 8 104.0 9 103.0 10 77.0 11 94.0 12 86.0 13 99.0 14 135.0 15 214.0 16 310.0 17 421.0 18 496.0 19 633.0 20 737.0 21 1047.0 22 1256.0 23 1487.0 24 1758.0 25 1940.0 26 2001.0 27 2133.0 28 2283.0 29 2322.0 30 2582.0 31 3043.0 32 3766.0 33 4527.0 34 5195.0 35 6411.0 36 7288.0 37 6838.0 38 3752.0 39 1034.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 100.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.43710192310658 0.48733093780361797 0.0678562065296177 0.007710932560183828 8 99.4000894468177 0.45648720756288264 0.07865151211387506 0.06477183350554416 9 97.53787294437075 1.396130943198297 0.4442936040233254 0.6217025084076394 10 43.363200345449776 38.01335533519424 9.186805052203013 9.43663926715297 11 31.90678880972209 27.48218747108356 23.131612226643224 17.479411492551126 12 33.65050969264223 14.30069552611693 21.686226732260998 30.362568048979842 13 26.81091251175917 13.47099918264115 25.864009993368597 33.85407831223108 14 21.328191284226765 17.243347473968377 28.882375626687235 32.546085615117626 15 24.83200741484514 18.10612497103576 27.223294971808144 29.83857264231096 16 30.181896724624714 18.81913695481124 25.155437616674636 25.84352870388941 17 29.758746220768806 22.66921700499784 23.076448448201393 24.49558832603196 18 29.186588111909685 24.23732996082544 23.54946173540208 23.026620191862797 19 28.795100806762612 26.043161028583768 20.8462523331328 24.315485831520817 20 25.52991268396532 27.78840517108389 23.376322853352256 23.30535929159853 21 25.692473781616286 22.79919802590993 28.11844540407156 23.389882788402222 22 24.253967274802214 23.074194593094088 26.72763444010919 25.94420369199451 23 24.244714155729994 24.16143608408001 28.161867896303377 23.43198186388662 24 23.92239717471431 26.456209613990715 24.73358728004565 24.887805931249325 25 27.574294835217373 26.158567617167623 23.421186558302363 22.845950989312648 26 22.70964951574785 25.553375863080984 25.967345803920356 25.769628817250805 27 22.048228088310164 21.10094573954364 29.447521483561413 27.403304688584782 28 20.97157862218103 25.261044176706825 28.63453815261044 25.132839048501697 29 23.411706624703015 24.26949304205622 29.71088277947484 22.60791755376593 30 23.447648138720474 22.37546474136468 33.24385615772666 20.93303096218818 31 21.04758813619573 24.26237616111034 35.27302514644287 19.417010556251064 32 26.79755698818594 20.383108670841175 31.483080909343286 21.3362534316296 33 24.532179556517036 22.191145793092794 33.10515336475315 20.171521285637024 34 20.740066071814507 23.389113587946525 37.73812096699496 18.132699373244 35 21.19898156006481 25.24959493866214 36.79191420415091 16.759509297122136 36 21.62491892392748 26.131204249930505 33.753899373011706 18.489977453130308 37 19.99227202472952 25.67387944358578 35.85625965996908 18.47758887171561 38 24.61227137913989 24.567474048442904 34.43524468610974 16.385009886307465 39 19.195765230312038 20.678553739474985 29.097015849430413 31.028665180782568 40 13.948404700629439 15.83051688319742 25.33874918607175 44.88232923010139 41 15.217154675084357 17.283739445489747 27.478268982568537 40.02083689685736 42 18.969088098918082 25.63214837712519 36.5564142194745 18.842349304482227 43 12.63038969882688 13.955179991952207 28.71513913393382 44.69929117528709 44 14.103537505616934 15.39117095619567 25.75886700652339 44.74642453166401 45 19.403699471945156 22.636877373930766 36.36939134731186 21.590031806812217 46 19.45829153484065 23.270314067443476 35.35303649972992 21.918357897985956 47 17.18468016843794 23.044531165646063 34.73184124878531 25.038947417130693 48 19.158489168672467 27.439363080910944 35.258902672344625 18.143245078071963 49 14.660293298682012 16.001485056617785 25.51358208031681 43.824639564383396 50 18.85083760895098 24.39574704827842 36.922791617728876 19.830623725041725 51 18.953297638575098 27.485995481415 34.64454829624586 18.91615858376404 52 18.356919456228905 26.892825070448705 35.38228098968817 19.367974483634224 53 20.696369789976355 25.32183535011668 35.05957624368307 18.9222186162239 54 16.476456689265362 23.098543696817135 32.132874152548915 28.292125461368585 55 20.28116792831763 25.288119882589218 35.359184304032134 19.071527885061023 56 19.768843289347632 24.59748447108996 33.56098766958188 22.07268456998053 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 3.5 9 6.0 10 20.5 11 35.0 12 57.0 13 116.0 14 153.0 15 194.0 16 235.0 17 290.5 18 346.0 19 381.5 20 417.0 21 531.0 22 745.5 23 846.0 24 1090.5 25 1335.0 26 1520.5 27 1706.0 28 1918.5 29 2178.0 30 2225.0 31 2203.0 32 2181.0 33 2024.0 34 1867.0 35 1785.5 36 1704.0 37 1635.0 38 1558.0 39 1550.0 40 1581.5 41 1613.0 42 1670.0 43 1727.0 44 1854.5 45 1982.0 46 2206.5 47 2471.5 48 2512.0 49 2547.0 50 2582.0 51 2694.5 52 2807.0 53 2887.5 54 3103.0 55 3238.0 56 3426.5 57 3615.0 58 4001.5 59 4388.0 60 4162.5 61 3937.0 62 3554.5 63 2889.0 64 2606.0 65 2343.5 66 2081.0 67 1793.0 68 1505.0 69 1290.0 70 1075.0 71 870.5 72 541.5 73 417.0 74 335.0 75 253.0 76 187.0 77 121.0 78 96.0 79 50.5 80 30.0 81 21.5 82 13.0 83 8.0 84 3.0 85 1.5 86 0.0 87 1.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.03238591675277208 10 0.0 11 0.0015421865120367657 12 0.0 13 0.0 14 0.02775935721666178 15 0.1665561432999707 16 0.04009684931295591 17 0.023132797680551487 18 0.007710932560183828 19 0.02467498419258825 20 0.03238591675277208 21 0.004626559536110297 22 0.0 23 0.0 24 0.0 25 0.0 26 0.16038739725182363 27 0.04009684931295591 28 0.15884521073978689 29 0.03855466280091914 30 0.03392810326480885 31 0.21899048470922075 32 0.007710932560183828 33 0.197399873540706 34 0.10024212328238977 35 0.05860308745739709 36 0.13725459957127215 37 0.2205326712212575 38 0.16501395678793393 39 0.36241383032863994 40 0.525885600604537 41 0.8235275974276328 42 0.2205326712212575 43 0.35316071125641935 44 0.47190907268325033 45 0.11874836142683097 46 0.07402495257776476 47 0.01850623814444119 48 0.04780778187313974 49 0.3068951158953164 50 0.20819517912496338 51 0.34082321916012526 52 0.39634193359344877 53 0.20973736563700013 54 0.13879678608330892 55 0.17426707586015452 56 0.1927733140045957 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 64843.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.90771555911971 #Duplication Level Percentage of deduplicated Percentage of total 1 94.0738921441353 82.69820952145952 2 4.120908038314445 7.245192233548725 3 0.9052313953896355 2.387304720632913 4 0.2929721764148627 1.0301805900405594 5 0.1701694677379741 0.7479604583378313 6 0.09648784253184099 0.5089215489721327 7 0.07543594961580295 0.4641981401230665 8 0.05438405669976492 0.38246225498511793 9 0.03333216378372689 0.26371389355828695 >10 0.16139784568962492 2.652560800703237 >50 0.007017297638679344 0.45957158058695624 >100 0.008771622048349182 1.159724257051648 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TAGCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC 270 0.41639035824992676 No Hit TAGCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 169 0.2606295205342134 No Hit TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 107 0.16501395678793393 No Hit TAGCATGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC 105 0.1619295837638604 No Hit TAGCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTCCCTACCAAGCGC 101 0.15576083771571334 No Hit TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 86 0.13262804003516185 No Hit TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 75 0.11566398840275743 No Hit TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 71 0.10949524235461038 No Hit TAGCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG 66 0.10178430979442654 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.004626559536110297 0.0 0.0 0.0 0.0 11 0.01079530558425736 0.0 0.0 0.0 0.0 12 0.012337492096294126 0.0 0.0 0.0 0.0 13 0.013879678608330892 0.0 0.0 0.0 0.0 14 0.01696405163240442 0.0 0.0 0.0 0.0 15 0.02159061116851472 0.0 0.0 0.0 0.0 16 0.02467498419258825 0.0 0.0 0.0 0.0 17 0.02930154372869855 0.0 0.0 0.0 0.0 18 0.03547028977684561 0.0 0.0 0.0 0.0 19 0.041639035824992676 0.0 0.0 0.0 0.0 20 0.0493499683851765 0.0 0.0 0.0 0.0 21 0.05551871443332357 0.0 0.0 0.0 0.0 22 0.05706090094536033 0.0 0.0 0.0 0.0 23 0.06322964699350739 0.0 0.0 0.0 0.0 24 0.06477183350554416 0.0 0.0 0.0 0.0 25 0.06939839304165446 0.0 0.0 0.0 0.0 26 0.07865151211387506 0.0 0.0 0.0 0.0 27 0.08173588513794858 0.0 0.0 0.0 0.0 28 0.08636244467405887 0.0 0.0 0.0 0.0 29 0.08944681769813241 0.0 0.0 0.0 0.0 30 0.08944681769813241 0.0 0.0 0.0 0.0 31 0.09098900421016917 0.0 0.0 0.0 0.0 32 0.09098900421016917 0.0 0.0 0.0 0.0 33 0.09098900421016917 0.0 0.0 0.0 0.0 34 0.09098900421016917 0.0 0.0 0.0 0.0 35 0.09098900421016917 0.0 0.0 0.0 0.0 36 0.09098900421016917 0.0 0.0 0.0 0.0 37 0.09098900421016917 0.0 0.0 0.0 0.0 38 0.09098900421016917 0.0 0.0 0.0 0.0 39 0.09407337723424271 0.0 0.0 0.0 0.0 40 0.09407337723424271 0.0 0.0 0.0 0.0 41 0.09407337723424271 0.0 0.0 0.0 0.0 42 0.09561556374627947 0.0 0.0 0.0 0.0 43 0.10024212328238977 0.0 0.0 0.0 0.0 44 0.10178430979442654 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATGCTT 60 0.0 50.30625 35 TATGCCC 25 1.9919818E-5 50.30625 35 GTAAGCC 15 0.008194717 50.30625 35 ATACAAA 20 4.0308275E-4 50.30625 36 TACCCGT 15 0.008220079 50.26698 49 CACGATT 25 2.0012229E-5 50.26698 47 CGATTAA 30 9.950891E-7 50.26698 49 ACGATTA 30 9.950891E-7 50.26698 48 AAGATTA 60 0.0 50.26698 48 TGTACAT 15 0.008220079 50.26698 46 AAAGATT 60 0.0 50.26698 47 CATTTGA 20 4.0464132E-4 50.266975 46 AAGGACA 15 0.008245501 50.22777 45 GTAACTT 15 0.008245501 50.22777 45 CGACTCC 25 2.0104995E-5 50.227768 44 CCAAAAC 120 0.0 50.18862 43 TGATACA 20 4.1250617E-4 50.071537 34 TGATATG 20 4.156859E-4 49.993786 50 TCTATTA 20 4.156859E-4 49.993786 50 ATCTCAT 25 2.0764382E-5 49.955006 12 >>END_MODULE