FastQCFastQC Report
Fri 17 Jun 2016
SRR1526327_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526327_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14685
Sequences flagged as poor quality0
Sequence length56
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCTAGGGTCCAGAGTTCTTTGTCATTACCTTTATGTTCCTTTATGCTGCTGGCT380.2587674497786857No Hit
CCGCTAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC260.17705141300646918No Hit
CCGCTAGGGATGAGATGGACTTAAATGAATTGACGGGTACCCACAGTTCTGAGTCG260.17705141300646918No Hit
CCGCTAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG240.1634320735444331No Hit
CCGCTAGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG230.15662240381341505No Hit
CCGCTAGGGTCCAGAGTTCTTTGTCATTACCTTTATGTTACTTTATGCTGCTGGCT230.15662240381341505No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC220.149812734082397No Hit
CCGCTAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGCGCTAATACATGCCGAC210.14300306435137897No Hit
CCGCTAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG210.14300306435137897No Hit
CCGCTAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG200.1361933946203609No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTCCTCTTCC180.12257405515832481No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC150.10214504596527069No Hit
CCGCTAGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCCGTGCCATGAAGGCCT150.10214504596527069No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCCAG150.00805454750.32758740
GTACCCA150.00805454750.32758737
ACCCACA150.00805454750.32758739
TACCCAC150.00805454750.32758738
GTTACTT150.00805454750.32758737
CCGGAGA150.00816546550.1546436
AGCTCTG150.00816546550.1546445
CAGTTCT309.57094E-750.1546444
TATGCTG203.9869142E-450.1546444
TATGTTC150.00816546550.1546434
TATGTTA150.00816546550.1546434
GTTCTGA309.57094E-750.1546446
GGCCGGA150.00816546550.1546434
TGCTGCT203.9869142E-450.1546446
TGTTACT150.00816546550.1546436
AGAGCCC150.00816546550.1546436
GCCGGAG150.00816546550.1546435
ATGCTGC203.9869142E-450.1546445
ACCCTGC251.9507124E-550.1546442
TCTGAGT354.7062713E-850.1546448