##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526327_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14685 Sequences flagged as poor quality 0 Sequence length 56 %GC 56 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.2789240721825 39.0 38.0 39.0 33.0 40.0 2 36.9190330268982 39.0 38.0 39.0 32.0 40.0 3 35.939121552604696 38.0 35.0 39.0 28.0 40.0 4 35.87654068777664 38.0 35.0 39.0 27.0 40.0 5 36.16779026217228 38.0 36.0 39.0 29.0 40.0 6 34.82499148791284 38.0 35.0 39.0 18.0 40.0 7 36.48144364998298 38.0 36.0 39.0 31.0 40.0 8 36.406741573033706 38.0 36.0 39.0 30.0 40.0 9 36.48192032686415 38.0 36.0 39.0 31.0 40.0 10 35.613891726251275 38.0 35.0 39.0 28.0 40.0 11 34.47579162410623 38.0 33.0 39.0 26.0 40.0 12 32.01641130405175 35.0 29.0 39.0 17.0 40.0 13 31.534218590398364 35.0 28.0 39.0 15.0 40.0 14 30.895471569628874 35.0 26.0 39.0 12.0 40.0 15 31.75056179775281 36.0 28.0 39.0 16.0 40.0 16 32.122574055158324 36.0 29.0 38.0 17.0 40.0 17 32.261967994552265 36.0 29.0 38.0 19.0 40.0 18 32.357916241062306 35.0 29.0 38.0 19.0 40.0 19 33.28879809329248 36.0 30.0 38.0 23.0 40.0 20 33.368947906026555 36.0 30.0 38.0 23.0 40.0 21 35.06189989785496 38.0 34.0 39.0 27.0 40.0 22 34.69451821586653 38.0 33.0 39.0 26.0 40.0 23 34.80660537963909 38.0 33.0 39.0 27.0 40.0 24 34.400408580183864 38.0 33.0 39.0 24.0 40.0 25 34.74211780728635 38.0 33.0 39.0 26.0 40.0 26 35.11161048689139 38.0 35.0 39.0 28.0 40.0 27 35.104528430371126 38.0 35.0 39.0 28.0 40.0 28 34.88130745658835 38.0 35.0 39.0 27.0 40.0 29 34.8388151174668 38.0 35.0 39.0 27.0 40.0 30 34.5297923050732 38.0 34.0 39.0 27.0 40.0 31 34.20415389853592 38.0 33.0 39.0 25.0 40.0 32 33.021722846441946 37.0 32.0 39.0 18.0 40.0 33 34.25808648280558 38.0 33.0 39.0 26.0 40.0 34 33.9919645897174 38.0 33.0 39.0 24.0 40.0 35 34.05325161729656 38.0 33.0 39.0 25.0 40.0 36 32.070003404834864 35.0 31.0 38.0 22.0 39.0 37 31.802110997616616 35.0 31.0 38.0 19.0 39.0 38 30.99938712972421 34.0 30.0 38.0 13.0 39.0 39 29.10875042560436 33.0 27.0 38.0 5.0 39.0 40 25.300578821927136 31.0 12.0 37.0 4.0 39.0 41 21.658495062989445 25.0 9.0 32.0 4.0 36.0 42 25.144773578481445 30.0 20.0 33.0 4.0 36.0 43 21.294041538985358 25.0 7.0 33.0 4.0 36.0 44 22.121756894790604 27.0 8.0 33.0 4.0 36.0 45 24.549336057201227 30.0 18.0 33.0 4.0 36.0 46 24.490432414027918 30.0 11.0 35.0 4.0 38.0 47 23.82165474974464 29.0 5.0 35.0 4.0 38.0 48 23.80749063670412 29.0 4.0 35.0 4.0 38.0 49 21.296493020088526 25.0 4.0 35.0 4.0 38.0 50 23.677970718420156 29.0 4.0 35.0 4.0 38.0 51 25.097786857337418 31.0 4.0 37.0 4.0 39.0 52 24.866734763363976 31.0 4.0 37.0 4.0 39.0 53 24.47211440245148 31.0 4.0 37.0 4.0 39.0 54 23.597208035410283 30.0 4.0 36.0 4.0 39.0 55 23.83003064351379 30.0 4.0 36.0 4.0 39.0 56 23.265645216207012 30.0 4.0 37.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.5878088492704237 1 2 2.282363908415583 1 3 2.6749601895629667 1 4 3.1643184364346695 1 5 2.6446955957067146 1 6 3.835159273897162 1 7 2.2256724249991393 1 8 2.497980981321689 1 9 2.0007063609067544 1 10 3.2259483138665743 1 11 -5.4475606939329 1 12 -8.84804637065216 1 13 -22.118976009794093 1 14 -18.103470065232486 1 15 -12.09472061341912 1 16 -22.114085461289605 1 17 -21.54726901866057 1 18 -20.984676579370475 1 19 -7.884168974920009 1 20 -10.443906470472925 1 21 -11.243773426870202 1 22 -4.552256984882881 1 23 -4.97528911839931 1 24 -3.163465428089495 1 25 -4.825546065312526 1 26 2.1447947778180776 1 27 2.3995124401508576 1 28 2.3465102639065663 1 29 2.510083663334477 1 30 2.364920440306534 1 31 -0.9341150355062737 1 32 -9.955275466797623 1 33 -1.7639738567373904 1 34 -0.9465333896129344 1 35 -1.198300515695987 1 36 0.8410170016365441 1 37 0.25173912237260865 1 38 0.7540953024888886 1 39 3.5140735409129604 1 40 7.092176424782387 1 41 0.15295740355206888 1 42 -3.890761730011242 1 43 -12.857233568381435 1 44 -15.818646924252945 1 45 -14.270523627028508 1 46 -18.344900239593414 1 47 -17.66761467746439 1 48 -17.307007607512855 1 49 -15.092456209190551 1 50 -17.287175623331787 1 51 -18.63727279920186 1 52 -18.352940144382966 1 53 -17.955641056762836 1 54 -17.24842503539931 1 55 -17.70817393268123 1 56 -16.930039929136043 2 1 1.1354278968894675 2 2 1.782363908415583 2 3 1.889245903848682 2 4 2.140508912625144 2 5 1.5494575004686197 2 6 3.3113497500876363 2 7 1.3923390916658036 2 8 1.6170286003693093 2 9 1.6673730275734187 2 10 1.964043551961808 2 11 -3.75708450345671 2 12 -9.300427323033112 2 13 -13.142785533603615 2 14 -15.698708160470582 2 15 -10.90424442294293 2 16 -3.7807521279562692 2 17 -4.118697590089141 2 18 -10.556105150799048 2 19 -3.7175023082533443 2 20 -4.34866837523483 2 21 2.2562265731298012 2 22 2.8763144436885497 2 23 1.8104251673149747 2 24 3.0032012385771694 2 25 2.5315967918303315 2 26 -0.236157603134302 2 27 0.2804648211032372 2 28 0.8227007400970408 2 29 -0.7994401461893332 2 30 1.2458728212589136 2 31 -4.07697217836342 2 32 -8.955275466797623 2 33 -3.049688142451675 2 34 -1.4227238658034125 2 35 -1.198300515695987 2 36 3.9124455730651135 2 37 4.180310550944039 2 38 3.730285778679363 2 39 5.395025921865344 2 40 8.354081186687146 2 41 5.581528832123496 2 42 -1.1526664919160048 2 43 -1.0715192826671505 2 44 -7.961504067110088 2 45 1.5628097063048259 2 46 -10.82109071578389 2 47 -10.381900391750102 2 48 -9.307007607512855 2 49 -10.854360971095314 2 50 -8.144318480474645 2 51 -10.113463275392336 2 52 -10.019606811049632 2 53 -9.812783913905694 2 54 -16.010329797304074 2 55 -10.51769774220504 2 56 -9.025278024374138 3 1 1.9635852467859465 3 2 2.3537924798441523 3 3 2.5786868976375033 3 4 3.161212846372557 3 5 2.5950061547129266 3 6 4.7792586527791485 3 7 2.104554412576775 3 8 2.4513971303900135 3 9 2.2243088453788005 3 10 2.887438997096382 3 11 2.071072846439769 3 12 0.7389101510869693 3 13 -4.991646817247506 3 14 -5.957507332313234 3 15 -2.604037383605455 3 16 1.7616909362259214 3 17 1.4030415403456402 3 18 0.6674973336730012 3 19 1.0754583542725342 3 20 0.7113730326326646 3 21 2.6972203619496753 3 22 1.9694821455519005 3 23 0.9781270306690146 3 24 2.3179010315378363 3 25 2.059547102389338 3 26 1.641689187755965 3 27 0.8995124401508576 3 28 1.191230760800977 3 29 3.0970401850736096 3 30 2.3183365893748586 3 31 2.8795495607670176 3 32 3.1751593158110722 3 33 3.937889497299878 3 34 4.587628101070294 3 35 3.9010783662915927 3 36 3.7944331507048688 3 37 5.85732918448441 3 38 5.4963313273336105 3 39 8.628980373211096 3 40 8.983480772608473 3 41 8.32687044703033 3 42 5.969486717193725 3 43 2.655188791867012 3 44 2.939117050902336 3 45 5.39717823632553 3 46 -0.5188132830716761 3 47 -6.145875547029606 3 48 -0.9591815205563314 3 49 -10.657673600494899 3 50 -0.2871756233317875 3 51 -2.7242293209409887 3 52 -1.3529401443829663 3 53 -2.955641056762836 3 54 -7.813642426703659 3 55 -1.4473043674638397 3 56 1.7656122447769995 4 1 2.6157591598294303 4 2 3.179879436365894 4 3 3.404773854159245 4 4 3.9872998028942988 4 5 3.8123974590607546 4 6 4.909693435387844 4 7 2.626293543011556 4 8 3.190527565172623 4 9 2.876482758422277 4 10 3.4091781275311703 4 11 4.244985889918034 4 12 5.130214498913059 4 13 6.00835318275249 4 14 5.912057885078074 4 15 7.048136529438022 4 16 5.805169197095491 4 17 5.229128496867382 4 18 5.493584290194743 4 19 4.336327919489925 4 20 4.885286076110923 4 21 3.3059160141235893 4 22 3.491221275986682 4 23 3.5868226828429286 4 24 2.6657271184943525 4 25 3.059547102389338 4 26 2.1199500573211836 4 27 4.029947222759553 4 28 2.0607959781922816 4 29 2.183996706812735 4 30 2.4922496328531167 4 31 2.8360712998974478 4 32 3.4795071418980257 4 33 1.6335416712129245 4 34 3.196323753244208 4 35 2.031513148900288 4 36 4.8379114115744315 4 37 4.074720488832238 4 38 6.23546176211622 4 39 7.542023851471971 4 40 10.41826338130413 4 41 5.457305229639026 4 42 8.969486717193728 4 43 6.089971400562664 4 44 9.852160529163204 4 45 8.13630867110814 4 46 8.61162149953702 4 47 9.767167931231263 4 48 7.997340218574102 4 49 6.864065529939882 4 50 9.930215681016037 4 51 8.667075026885097 4 52 8.560103333877905 4 53 7.479141551932816 4 54 6.403748877644166 4 55 6.683130415144856 4 56 5.243873114342218 5 1 2.8092374206989916 5 2 3.4037924798441495 5 3 4.217817332420111 5 4 4.228604150720386 5 5 4.223267024278144 5 6 5.642302131040019 5 7 3.182815282141995 5 8 3.162266695607407 5 9 3.2221349323353223 5 10 4.533091171009431 5 11 5.416725020352814 5 12 8.251953629347842 5 13 7.39530970449162 5 14 8.625101363338938 5 15 8.19813652943802 5 16 6.507343110138965 5 17 7.23130240991086 5 18 6.0153234206295245 5 19 5.0015453107942776 5 20 5.7418078152413585 5 21 3.227655144558369 5 22 4.497743015117116 5 23 3.3389965958864067 5 24 4.472248857624791 5 25 4.803025363258904 5 26 3.2590804921037915 5 27 2.949512440150855 5 28 4.360795978192279 5 29 3.4100836633344755 5 30 4.779206154592252 5 31 3.7230278216365775 5 32 5.894724533202378 5 33 4.007454714691185 5 34 4.3463237532442065 5 35 4.344556627161154 5 36 5.805302715922259 5 37 4.8660248366583225 5 38 6.83980958820317 5 39 8.985502112341532 5 40 9.54217642478239 5 41 3.102957403552068 5 42 12.7520954128459 5 43 14.48562357447571 5 44 14.845638790032773 5 45 12.508047801542922 5 46 9.455099760406586 5 47 10.98238532253561 5 48 11.492992392487142 5 49 11.407543790809449 5 50 12.312824376668214 5 51 11.11272720079814 5 52 12.147059855617034 5 53 11.444358943237162 5 54 11.651574964600687 5 55 12.29182606731877 5 56 12.919960070863958 6 1 2.6175707540323288 6 2 3.4787924798441523 6 3 3.5261506657534483 6 4 4.045270817387049 6 5 3.656600357611481 6 6 5.400635464373352 6 7 3.3328152821419934 6 8 3.337266695607404 6 9 2.9054682656686595 6 10 2.4997578376760927 6 11 3.7250583536861512 6 12 6.068620296014505 6 13 5.428643037824958 6 14 8.450101363338941 6 15 6.7564698627713575 6 16 6.624009776805632 6 17 5.381302409910859 6 18 5.34865675396286 6 19 5.276545310794276 6 20 5.34180781524136 6 21 3.869321811225035 6 22 4.822743015117119 6 23 4.03066326255307 6 24 2.9555821909581255 6 25 3.019692029925565 6 26 3.025747158770457 6 27 2.1078457734841933 6 28 2.4357959781922816 6 29 2.885083663334477 6 30 3.787539487925585 6 31 3.506361154969916 6 32 3.794724533202377 6 33 3.1824547146911826 6 34 4.029657086577544 6 35 4.694556627161155 6 36 4.846969382588924 6 37 3.341024836658324 6 38 3.373142921536509 6 39 5.543835445674869 6 40 0.9671764247823873 6 41 0.986290736885401 6 42 7.2104287461792325 6 43 11.74395690780904 6 44 12.187305456699434 6 45 6.574714468209589 6 46 10.613433093739921 6 47 9.540718655868943 6 48 10.817992392487145 6 49 10.657543790809449 6 50 9.671157710001545 6 51 7.4460605341314725 6 52 8.188726522283702 6 53 9.127692276570496 6 54 8.501574964600689 6 55 9.29182606731877 6 56 8.153293404197289 7 1 2.609237420698996 7 2 2.553792479844155 7 3 3.5178173324201154 7 4 4.328604150720388 7 5 3.973267024278144 7 6 5.292302131040017 7 7 3.2828152821419962 7 8 3.662266695607407 7 9 3.372134932335321 7 10 3.5330911710094313 7 11 5.316725020352813 7 12 7.501953629347842 7 13 8.245309704491621 7 14 9.425101363338943 7 15 8.748136529438025 7 16 6.157343110138971 7 17 6.431302409910856 7 18 6.615323420629526 7 19 4.551545310794275 7 20 4.391807815241357 7 21 3.0776551445583706 7 22 3.3477430151171177 7 23 3.5889965958864067 7 24 4.12224885762479 7 25 3.5030253632588995 7 26 2.7090804921037943 7 27 2.749512440150859 7 28 2.5607959781922816 7 29 3.310083663334474 7 30 2.529206154592252 7 31 3.4230278216365804 7 32 4.094724533202374 7 33 2.3074547146911826 7 34 1.8963237532442108 7 35 3.1445566271611582 7 36 -1.4446972840777406 7 37 -0.08397516334167676 7 38 0.339809588203174 7 39 3.0855021123415334 7 40 -2.9578235752176134 7 41 -10.247042596447931 7 42 6.602095412845902 7 43 11.285623574475707 7 44 8.69563879003277 7 45 6.408047801542921 7 46 10.855099760406588 7 47 10.882385322535608 7 48 11.192992392487145 7 49 13.357543790809451 7 50 10.862824376668211 7 51 12.562727200798143 7 52 12.047059855617032 7 53 9.744358943237163 7 54 8.701574964600688 7 55 9.141826067318771 7 56 7.119960070863957 8 1 2.409237420698993 8 2 1.7537924798441509 8 3 2.2678173324201154 8 4 2.378604150720385 8 5 1.8732670242781424 8 6 3.442302131040016 8 7 1.6828152821419948 8 8 1.912266695607407 8 9 1.1721349323353252 8 10 2.18309117100943 8 11 3.2167250203528184 8 12 5.90195362934784 8 13 6.845309704491623 8 14 5.97510136333894 8 15 6.298136529438022 8 16 6.107343110138967 8 17 5.23130240991086 8 18 4.865323420629526 8 19 3.901545310794276 8 20 3.291807815241363 8 21 1.2776551445583735 8 22 1.6977430151171191 8 23 2.4889965958864053 8 24 2.2722488576247883 8 25 2.0030253632588995 8 26 1.2590804921037915 8 27 1.3495124401508605 8 28 1.7607959781922844 8 29 2.3600836633344784 8 30 2.5792061545922493 8 31 3.1730278216365804 8 32 3.094724533202374 8 33 2.657454714691184 8 34 1.946323753244208 8 35 2.8945566271611582 8 36 2.3553027159222566 8 37 0.9160248366583232 8 38 3.3398095882031704 8 39 4.6355021123415305 8 40 -2.4578235752176134 8 41 -9.397042596447932 8 42 6.102095412845902 8 43 9.935623574475706 8 44 8.795638790032768 8 45 6.308047801542919 8 46 10.355099760406588 8 47 10.082385322535611 8 48 9.092992392487144 8 49 13.25754379080945 8 50 10.812824376668214 8 51 12.662727200798138 8 52 12.147059855617034 8 53 13.144358943237165 8 54 13.901574964600687 8 55 12.741826067318772 8 56 13.169960070863958 9 1 2.833150464177251 9 2 3.5277055233224104 9 3 3.4917303758983707 9 4 4.204691107242127 9 5 4.116745285147708 9 6 5.561867348431321 9 7 3.3219457169246027 9 8 3.8427014782160995 9 9 3.5721349323353238 9 10 4.017873779705077 9 11 4.418898933396292 9 12 7.869344933695668 9 13 7.790961878404669 9 14 8.738144841599809 9 15 7.961180007698889 9 16 7.544299631878101 9 17 6.44651980121521 9 18 6.754453855412134 9 19 5.379806180359495 9 20 5.624416510893532 9 21 3.0885247097757613 9 22 3.9260038846823377 9 23 2.717257465451624 9 24 4.361379292407399 9 25 4.059547102389338 9 26 2.859080492103793 9 27 2.812555918411725 9 28 2.3216655434096722 9 29 3.401388011160563 9 30 3.231380067635726 9 31 4.053462604245276 9 32 4.914289750593682 9 33 3.9813677581694407 9 34 5.152845492374645 9 35 2.5532522793350694 9 36 3.750954889835306 9 37 4.205155271440933 9 38 3.018070457768392 9 39 4.846371677558924 9 40 2.2008720769563013 9 41 -3.542694770360974 9 42 8.7520954128459 9 43 12.089971400562664 9 44 12.026073572641465 9 45 8.918917366760311 9 46 12.829012803884844 9 47 12.593254887753002 9 48 11.779948914226278 9 49 14.385804660374667 9 50 12.495433072320385 9 51 14.10185763558075 9 52 13.168798986051815 9 53 14.43566329106325 9 54 13.534183660252861 9 55 14.55269563253616 9 56 10.113438331733523 10 1 3.159237420698993 10 2 3.444701570753246 10 3 4.726908241511019 10 4 4.651331423447658 10 5 4.482357933369052 10 6 5.9195748583127425 10 7 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-6.122307723429502 114 54 -8.831758368732645 114 55 -8.791507266014564 114 56 -4.013373262469376 115 1 2.295601057062626 115 2 2.9901561162077854 115 3 3.9541809687837457 115 4 2.9240586961749315 115 5 3.7096306606417784 115 6 3.874120312858196 115 7 3.2191789185056265 115 8 3.3486303319710373 115 9 2.9357712959716906 115 10 3.5830911710094284 115 11 0.3121795658073623 115 12 -1.620773643379433 115 13 -0.9955993864174673 115 14 2.1887377269753046 115 15 2.502681983983475 115 16 -3.8153841625883054 115 17 -4.755061226452778 115 18 -0.8937674884613855 115 19 -1.007545598296634 115 20 -1.5218285483950034 115 21 2.0094733263765505 115 22 2.3568339242080256 115 23 2.784451141340952 115 24 3.1677034030793365 115 25 2.921207181440721 115 26 1.9499895830128864 115 27 3.2631488037872245 115 28 3.4244323418286484 115 29 3.2828109360617503 115 30 2.5792061545922493 115 31 3.2866641852729472 115 32 -6.864366375888533 115 33 3.6256365328730027 115 34 4.196323753244208 115 35 3.3991020817066087 115 36 6.328029988649533 115 37 7.284206654840144 115 38 8.357991406384993 115 39 10.221865748705167 115 40 12.86490369750966 115 41 10.062048312642979 115 42 6.66118632193681 115 43 -9.123467334615201 115 44 -0.6498157554217769 115 45 1.6580478015429208 115 46 -6.708536603229778 115 47 -8.940341950191662 115 48 -2.9433712438764914 115 49 -9.91063802737237 115 50 -2.3780847142408774 115 51 0.08999992807086699 115 52 1.8288780374352172 115 53 0.9534498523280739 115 54 0.6606658736915989 115 55 -0.7990830235903204 115 56 4.34268734359123 116 1 0.5823143437759128 116 2 1.199946325997999 116 3 2.087048101650879 116 4 1.8401426122588447 116 5 1.8424977935089117 116 6 -5.561267677478554E-6 116 7 1.6597383590650736 116 8 1.4814974648381707 116 9 1.725981086181477 116 10 1.9677065556248152 116 11 -3.079428825801031 116 12 -4.501892524498313 116 13 -13.212382603200684 116 14 -13.482590944353367 116 15 -5.336478855177361 116 16 -10.773426120630262 116 17 -8.964851436242988 116 18 -4.446215040908935 116 19 -5.098454689205724 116 20 -5.235115261681717 116 21 0.519962836866064 116 22 0.2169737843478856 116 23 0.6236119805017921 116 24 0.08378731916324966 116 25 0.02610228633582068 116 26 0.3206189536422528 116 27 0.28412782476624443 116 28 0.6761805935768948 116 29 0.31777597102678357 116 30 0.7330523084384026 116 31 0.30764320625196717 116 32 -9.416813928336083 116 33 0.038223945460416076 116 34 0.9655545224749744 116 35 1.4830181656226955 116 36 2.901456562076106 116 37 2.6198709905044772 116 38 4.30904035743394 116 39 6.43165595849538 116 40 8.74602257862854 116 41 8.922188172782839 116 42 3.2905569513074404 116 43 -7.829761040908908 116 44 1.7417926361866165 116 45 4.658047801542921 116 46 -0.3449002395934144 116 47 -8.513768523618236 116 48 -7.307007607512855 116 49 -5.784763901498243 116 50 -1.9025602387164042 116 51 2.7473425854135236 116 52 0.3393675479247271 116 53 0.19820509708331713 116 54 -1.709963496937771 116 55 -2.0927893172966137 116 56 1.2238062247101098 117 1 0.802094563556139 117 2 1.4966496227012982 117 3 2.3178173324201126 117 4 2.5214612935775307 117 5 2.216124167135291 117 6 3.2637307024685853 117 7 1.4399581392848475 117 8 1.5694095527502583 117 9 1.4292777894781779 117 10 2.4402340281522825 117 11 -8.233274979647184 117 12 -16.205189227795017 117 13 -13.761833152651235 117 14 -8.746327208089632 117 15 -5.237577756276263 117 16 -6.256942604146747 117 17 -1.8329833043748565 117 18 1.0153234206295245 117 19 0.47297388222284553 117 20 -0.44390647047292475 117 21 2.8276551445583706 117 22 3.1620287294028344 117 23 3.0961394530292594 117 24 -1.2348939995180643 117 25 2.3887396489731856 117 26 1.716223349246647 117 27 -1.529058988420573 117 28 1.9179388353351356 117 29 1.1529408061916229 117 30 1.5792061545922493 117 31 -2.6484007497919926 117 32 -13.098132609654765 117 33 -2.7639738567373904 117 34 -1.9465333896129344 117 35 -1.3411576585531293 117 36 0.7695884302079747 117 37 1.466024836658324 117 38 0.6826667310603156 117 39 4.156930683770106 117 40 6.020747853353814 117 41 6.0101002606949265 117 42 4.466381127131616 117 43 -10.50009071123858 117 44 -7.10436120996723 117 45 0.5151906586857784 117 46 -0.20204309673627208 117 47 -13.524757534607247 117 48 -5.878436178941426 117 49 -3.949599066333409 117 50 -0.14431848047464513 117 51 -1.9229870849161443 117 52 0.6470598556170337 117 53 -0.6699267710485515 117 54 -5.534139321113598 117 55 -2.7081739326812304 117 56 2.355674356578241 118 1 1.159237420698993 118 2 1.8537924798441523 118 3 2.6511506657534483 118 4 2.128604150720385 118 5 2.5732670242781452 118 6 4.35896879770668 118 7 2.166148615475329 118 8 2.3789333622740685 118 9 2.238801599001988 118 10 2.9997578376760927 118 11 -6.649941646313852 118 12 -3.931379703985492 118 13 -5.9880236288417095 118 14 3.158434696672277 118 15 -16.28519680389531 118 16 -6.625990223194364 118 17 -6.535364256755809 118 18 -3.5680099127038076 118 19 -2.2651213558723917 118 20 -3.074858851425308 118 21 2.8276551445583706 118 22 2.281076348450455 118 23 2.6556632625530696 118 24 -1.794417809041878 118 25 1.9363586965922366 118 26 3.275747158770457 118 27 2.9828457734841933 118 28 2.727462644858946 118 29 3.1767503300011413 118 30 3.662539487925585 118 31 1.0896944883032447 118 32 -8.371942133464291 118 33 1.7241213813578469 118 34 2.362990419910872 118 35 2.1945566271611554 118 36 4.305302715922259 118 37 4.54935816999166 118 38 3.373142921536509 118 39 4.335502112341533 118 40 8.592176424782387 118 41 6.402957403552069 118 42 1.5854287461792325 118 43 -5.881043092190959 118 44 -0.8543612099672302 118 45 -2.008618865123747 118 46 -6.428233572926748 118 47 -9.834281344131055 118 48 -6.890340940846189 118 49 -7.175789542523885 118 50 -3.9538422899984553 118 51 -1.1372727992018596 118 52 -0.5196068110496341 118 53 -4.705641056762836 118 54 -10.331758368732645 118 55 -7.45817393268123 118 56 -4.096706595802711 119 1 1.3865101479717197 119 2 2.081065207116879 119 3 2.3178173324201126 119 4 2.9240586961749315 119 5 2.436903387914512 119 6 2.510483949221836 119 7 1.0373607366874467 119 8 1.894084877425584 119 9 1.2084985686989569 119 10 2.128545716463975 119 11 -7.596911343283548 119 12 -9.711682734288523 119 13 0.09530970449162268 119 14 -0.3567168184792422 119 15 -6.2245907432892515 119 16 -9.906293253497395 119 17 -10.300515771907325 119 18 -2.166494761188659 119 19 -1.007545598296634 119 20 -1.3400103665768235 119 21 -2.808708491805266 119 22 -2.1886206212465176 119 23 -3.942821585931778 119 24 -6.4686602332843 119 25 -3.3515200912865524 119 26 -2.231828598805297 119 27 -2.1913966507582323 119 28 -2.666476749080445 119 29 -2.53537088212007 119 30 -2.2389756635895672 119 31 -4.349699451090693 119 32 -8.137093648615807 119 33 -2.919818012581544 119 34 -1.3491307922103388 119 35 -1.0554433728388446 119 36 1.0553027159222594 119 37 1.7387521093855973 119 38 3.2670823154759 119 39 4.31277483961426 119 40 6.592176424782387 119 41 9.516593767188432 119 42 5.84300450375499 119 43 -2.668921880069746 119 44 2.4410933354873166 119 45 4.385320528815647 119 46 -3.617627512320688 119 47 -9.667614677464389 119 48 -7.034280334785581 119 49 -8.183365300099643 119 50 -5.650811986968151 119 51 -2.910000071929133 119 52 -2.5347583262011497 119 53 -5.773822874944653 119 54 -6.430243217217493 119 55 -7.162719387226685 119 56 -2.8391308382269536 120 1 1.2592374206989945 120 2 1.9537924798441537 120 3 2.917817332420114 120 4 2.978604150720386 120 5 2.1732670242781467 120 6 3.192302131040016 120 7 1.3828152821419906 120 8 1.5122666956074013 120 9 1.7721349323353266 120 10 1.7830911710094313 120 11 -13.033274979647185 120 12 -5.14804637065216 120 13 -10.104690295508377 120 14 -5.77489863666106 120 15 -5.451863470561978 120 16 -13.242656889861031 120 17 -5.118697590089141 120 18 -4.584676579370477 120 19 -5.698454689205725 120 20 -7.358192184758639 120 21 -0.8723448554416322 120 22 -1.2522569848828837 120 23 -1.6610034041135933 120 24 -7.87775114237521 120 25 -2.0969746367410984 120 26 1.859080492103793 120 27 2.299512440150856 120 28 2.0607959781922816 120 29 1.6100836633344784 120 30 1.3792061545922465 120 31 -1.8769721783634203 120 32 -10.955275466797623 120 33 -0.7925452853088188 120 34 -10.903676246755793 120 35 -3.3554433728388453 120 36 -3.2446972840777413 120 37 -3.2339751633416753 120 38 -2.2601904117968274 120 39 0.8855021123415341 120 40 -6.207823575217613 120 41 -3.7470425964479297 120 42 -7.0479045871541 120 43 -4.614376425524293 120 44 -8.60436120996723 120 45 -13.14195219845708 120 46 -16.044900239593414 120 47 -17.16761467746439 120 48 -13.007007607512854 120 49 -14.692456209190551 120 50 -12.087175623331788 120 51 -11.837272799201859 120 52 -11.152940144382967 120 53 -11.555641056762836 120 54 -11.94842503539931 120 55 -11.20817393268123 120 56 -8.030039929136043 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 158.0 5 14.0 6 26.0 7 16.0 8 26.0 9 30.0 10 19.0 11 17.0 12 24.0 13 14.0 14 46.0 15 54.0 16 98.0 17 126.0 18 165.0 19 204.0 20 207.0 21 279.0 22 300.0 23 406.0 24 422.0 25 428.0 26 492.0 27 493.0 28 546.0 29 522.0 30 635.0 31 747.0 32 883.0 33 1068.0 34 1218.0 35 1392.0 36 1494.0 37 1244.0 38 685.0 39 187.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 0.0 100.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 100.0 0.0 6 0.0 100.0 0.0 0.0 7 99.59141981613891 0.35410282601293835 0.04085801838610827 0.013619339462036091 8 99.72761321075929 0.22471910112359553 0.020429009193054137 0.027238678924072182 9 99.4277539341917 0.37468492404114717 0.04768717215069146 0.14987396961645888 10 60.38134150493701 24.6033367381682 7.054817841334696 7.960503915560095 11 40.14573685644239 23.69926450558431 18.394170525742304 17.760828112231 12 39.918283963227786 10.24174327545114 18.869594824651003 30.970377936670072 13 25.352400408580184 15.253660197480423 22.138236295539667 37.25570309839973 14 27.454186252469515 13.95871653382383 25.771510320866543 32.81558689284011 15 24.768139661756685 20.376432078559738 25.05455537370431 29.800872885979267 16 31.682223887715473 16.576957143830484 26.483613817537645 25.257205150916402 17 33.555858310626704 19.67983651226158 21.57356948228883 25.190735694822884 18 35.04494688095887 21.35657858894034 22.6436938163988 20.95478071370199 19 29.440207028057753 26.60719150095342 18.31244892399891 25.64015254698992 20 29.1581528402125 22.21768151478 21.591063887753712 27.03310175725378 21 29.019955050057888 21.501055642579857 27.426275284342438 22.052714023019817 22 25.148110316649642 20.830779707184202 30.132788559754854 23.888321416411305 23 31.19509703779367 25.229826353421856 22.96901600272387 20.606060606060606 24 30.153217568947905 21.368743615934626 24.405856315968673 24.07218249914879 25 27.551923731699013 22.989445011916924 21.63432073544433 27.824310520939733 26 25.53191489361702 20.130932896890343 27.823240589198033 26.5139116202946 27 27.112292177705093 15.392477514309075 27.09866448623603 30.396565821749792 28 22.272231314784506 24.23622476813966 28.709765411893073 24.78177850518276 29 25.932988286570417 23.37237809861073 27.546989921002453 23.1476436938164 30 21.758975407044076 18.10750051093399 37.168744464881804 22.96477961714013 31 25.16199440692995 17.959211513539323 35.311370302162196 21.56742377736853 32 34.40245148110317 14.708886618998978 31.065713312904325 19.82294858699353 33 28.36390915910796 18.345495464775286 34.808702175543885 18.48189320057287 34 22.232063773250665 16.856305784560877 39.57893302446004 21.33269741772842 35 26.23475713604469 22.426595817153757 36.62374821173104 14.714898835070509 36 24.412025359601884 22.966800736246505 33.82643670325176 18.79473720089986 37 29.23318324464456 20.55532814845136 31.607313412471004 18.604175194433072 38 25.34260584986705 23.80173177882321 34.724210813390606 16.13145155791914 39 24.55409007038885 18.970819380851502 28.07353242670676 28.401558122052894 40 14.794520547945206 14.054794520547945 23.308219178082194 47.842465753424655 41 19.033232628398792 15.60010985992859 24.711617687448502 40.65503982422412 42 19.454297407912687 20.6343792633015 32.76261937244202 27.148703956343795 43 12.93297806927649 12.07897793263647 27.16403634624582 47.82400765184122 44 14.547693992062404 12.255371561516354 25.557684412207472 47.639250034213774 45 22.24569053621312 23.560673162090346 32.772364924712136 21.421271376984397 46 23.01089918256131 18.896457765667574 35.17029972752044 22.922343324250683 47 18.6729341235779 17.174194427413312 36.834934259826966 27.317937189181823 48 22.44856247445156 17.154925739201527 38.37716310123995 22.019348685106962 49 15.627989613229465 12.088287549542162 24.702747027470277 47.580975809758094 50 21.612771182971755 17.089643880474824 41.15158957565834 20.145995360895075 51 24.626203318085615 19.05509660681368 35.427049907830956 20.89165016726975 52 19.74870254028954 21.19639442775198 37.27123736683966 21.78366566511882 53 23.231703158038332 18.51851851851852 37.446286065070595 20.803492258372554 54 20.13363332651531 16.288266175768733 33.78332310629304 29.794777391422922 55 18.861622358554875 17.23926380368098 35.746421267893666 28.152692569870485 56 23.830947511929107 19.011588275391954 32.992501704158144 24.16496250852079 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 6.0 16 12.0 17 14.5 18 17.0 19 24.5 20 32.0 21 38.0 22 50.5 23 57.0 24 85.0 25 113.0 26 134.5 27 156.0 28 194.5 29 281.5 30 330.0 31 357.5 32 385.0 33 408.0 34 431.0 35 387.0 36 343.0 37 316.0 38 258.5 39 228.0 40 203.0 41 178.0 42 178.5 43 179.0 44 209.5 45 240.0 46 255.0 47 284.5 48 299.0 49 383.5 50 468.0 51 493.0 52 518.0 53 540.0 54 571.5 55 581.0 56 612.0 57 643.0 58 695.0 59 747.0 60 849.0 61 951.0 62 1035.0 63 1124.5 64 1130.0 65 1078.5 66 1027.0 67 937.5 68 848.0 69 758.0 70 668.0 71 591.5 72 444.5 73 374.0 74 320.0 75 266.0 76 228.0 77 190.0 78 162.5 79 94.0 80 53.0 81 42.0 82 31.0 83 24.0 84 17.0 85 10.5 86 4.0 87 2.5 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.04085801838610827 10 0.0 11 0.0068096697310180455 12 0.0 13 0.0 14 0.04085801838610827 15 0.14300306435137897 16 0.054477357848144364 17 0.034048348655090224 18 0.0068096697310180455 19 0.0068096697310180455 20 0.020429009193054137 21 0.013619339462036091 22 0.0 23 0.0 24 0.0 25 0.0 26 0.14300306435137897 27 0.061287027579162406 28 0.14300306435137897 29 0.0068096697310180455 30 0.04085801838610827 31 0.1634320735444331 32 0.0 33 0.149812734082397 34 0.054477357848144364 35 0.04085801838610827 36 0.10895471569628873 37 0.18386108273748722 38 0.12257405515832481 39 0.35410282601293835 40 0.5788219271365339 41 0.8239700374531835 42 0.17024174327545114 43 0.3268641470888662 44 0.48348655090228126 45 0.054477357848144364 46 0.034048348655090224 47 0.04085801838610827 48 0.047667688117126315 49 0.34729315628192037 50 0.18386108273748722 51 0.2587674497786857 52 0.2791964589717399 53 0.1634320735444331 54 0.12257405515832481 55 0.10214504596527069 56 0.10214504596527069 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 14685.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.63363976847123 #Duplication Level Percentage of deduplicated Percentage of total 1 93.31727406946926 81.77732379979571 2 4.553578366617453 7.98093292475315 3 0.9635558318439661 2.533197139938713 4 0.41184241199782423 1.4436499829758258 5 0.16318284248970394 0.7150153217568949 6 0.11655917320693139 0.6128702757916241 7 0.10101795011267385 0.6196799455226422 8 0.08547672701841635 0.599250936329588 9 0.06216489237703007 0.49029622063329925 >10 0.225347734866734 3.2277834525025537 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCGCTAGGGTCCAGAGTTCTTTGTCATTACCTTTATGTTCCTTTATGCTGCTGGCT 38 0.2587674497786857 No Hit CCGCTAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC 26 0.17705141300646918 No Hit CCGCTAGGGATGAGATGGACTTAAATGAATTGACGGGTACCCACAGTTCTGAGTCG 26 0.17705141300646918 No Hit CCGCTAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG 24 0.1634320735444331 No Hit CCGCTAGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG 23 0.15662240381341505 No Hit CCGCTAGGGTCCAGAGTTCTTTGTCATTACCTTTATGTTACTTTATGCTGCTGGCT 23 0.15662240381341505 No Hit CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 22 0.149812734082397 No Hit CCGCTAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGCGCTAATACATGCCGAC 21 0.14300306435137897 No Hit CCGCTAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG 21 0.14300306435137897 No Hit CCGCTAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG 20 0.1361933946203609 No Hit CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTCCTCTTCC 18 0.12257405515832481 No Hit CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 15 0.10214504596527069 No Hit CCGCTAGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCCGTGCCATGAAGGCCT 15 0.10214504596527069 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0068096697310180455 0.0 0.0 0.0 0.0 7 0.0068096697310180455 0.0 0.0 0.0 0.0 8 0.0068096697310180455 0.0 0.0 0.0 0.0 9 0.0068096697310180455 0.0 0.0 0.0 0.0 10 0.0068096697310180455 0.0 0.0 0.0 0.0 11 0.020429009193054137 0.0 0.0 0.0 0.0 12 0.020429009193054137 0.0 0.0 0.0 0.0 13 0.020429009193054137 0.0 0.0 0.0 0.0 14 0.020429009193054137 0.0 0.0 0.0 0.0 15 0.027238678924072182 0.0 0.0 0.0 0.0 16 0.03404834865509023 0.0 0.0 0.0 0.0 17 0.03404834865509023 0.0 0.0 0.0 0.0 18 0.04085801838610827 0.0 0.0 0.0 0.0 19 0.04766768811712632 0.0 0.0 0.0 0.0 20 0.04766768811712632 0.0 0.0 0.0 0.0 21 0.054477357848144364 0.0 0.0 0.0 0.0 22 0.054477357848144364 0.0 0.0 0.0 0.0 23 0.054477357848144364 0.0 0.0 0.0 0.0 24 0.054477357848144364 0.0 0.0 0.0 0.0 25 0.06128702757916241 0.0 0.0 0.0 0.0 26 0.06128702757916241 0.0 0.0 0.0 0.0 27 0.06128702757916241 0.0 0.0 0.0 0.0 28 0.06128702757916241 0.0 0.0 0.0 0.0 29 0.06809669731018046 0.0 0.0 0.0 0.0 30 0.06809669731018046 0.0 0.0 0.0 0.0 31 0.06809669731018046 0.0 0.0 0.0 0.0 32 0.06809669731018046 0.0 0.0 0.0 0.0 33 0.06809669731018046 0.0 0.0 0.0 0.0 34 0.06809669731018046 0.0 0.0 0.0 0.0 35 0.06809669731018046 0.0 0.0 0.0 0.0 36 0.06809669731018046 0.0 0.0 0.0 0.0 37 0.06809669731018046 0.0 0.0 0.0 0.0 38 0.06809669731018046 0.0 0.0 0.0 0.0 39 0.06809669731018046 0.0 0.0 0.0 0.0 40 0.06809669731018046 0.0 0.0 0.0 0.0 41 0.06809669731018046 0.0 0.0 0.0 0.0 42 0.06809669731018046 0.0 0.0 0.0 0.0 43 0.06809669731018046 0.0 0.0 0.0 0.0 44 0.06809669731018046 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCCCAG 15 0.008054547 50.327587 40 GTACCCA 15 0.008054547 50.327587 37 ACCCACA 15 0.008054547 50.327587 39 TACCCAC 15 0.008054547 50.327587 38 GTTACTT 15 0.008054547 50.327587 37 CCGGAGA 15 0.008165465 50.15464 36 AGCTCTG 15 0.008165465 50.15464 45 CAGTTCT 30 9.57094E-7 50.15464 44 TATGCTG 20 3.9869142E-4 50.15464 44 TATGTTC 15 0.008165465 50.15464 34 TATGTTA 15 0.008165465 50.15464 34 GTTCTGA 30 9.57094E-7 50.15464 46 GGCCGGA 15 0.008165465 50.15464 34 TGCTGCT 20 3.9869142E-4 50.15464 46 TGTTACT 15 0.008165465 50.15464 36 AGAGCCC 15 0.008165465 50.15464 36 GCCGGAG 15 0.008165465 50.15464 35 ATGCTGC 20 3.9869142E-4 50.15464 45 ACCCTGC 25 1.9507124E-5 50.15464 42 TCTGAGT 35 4.7062713E-8 50.15464 48 >>END_MODULE