FastQCFastQC Report
Fri 17 Jun 2016
SRR1526326_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526326_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54512
Sequences flagged as poor quality0
Sequence length56
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTTCGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC740.1357499266216613No Hit
TACTTCGGGAATTGACGGGTACGCACAGTTCTGAGTCGGCCACACGCAGGGATGAG580.10639859113589667No Hit
TACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA580.10639859113589667No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAGA150.00806612750.4967440
GCGCTCC150.00806612750.4967438
GGCCAAC203.9505758E-450.4967438
AGACCCT150.00806612750.4967440
AGACCCG150.00806612750.4967440
AGCGATA150.00806612750.4967438
AGCGCTC251.954441E-550.44972237
TGCCCAG150.00809592350.4497241
CCACAGT150.00809592350.4497241
TTTGTTC150.00809592350.4497237
TATCTTT204.0239142E-450.30919346
GCACGGA150.00818580250.30919346
TACGAAG150.00818580250.30919347
CGCCGGA150.00818580250.30919347
TTATCTT150.00821592650.26252445
CGCACGG150.00821592650.26252445
CTGCGCA150.00824613250.21594244
ATTCTCT150.00824613250.21594244
ATTTTCT150.00824613250.21594244
TTGCCTA150.00824613250.21594242
Produced by FastQC (version 0.11.5)