##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526326_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 54512 Sequences flagged as poor quality 0 Sequence length 56 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.78767977692985 39.0 38.0 40.0 35.0 40.0 2 36.01254769592016 39.0 36.0 39.0 28.0 40.0 3 37.3431904901673 39.0 38.0 40.0 33.0 40.0 4 37.51711549750514 39.0 38.0 40.0 34.0 40.0 5 37.5943095098327 39.0 38.0 40.0 35.0 40.0 6 37.33440343410625 39.0 38.0 40.0 33.0 40.0 7 37.11320443205166 39.0 38.0 39.0 33.0 40.0 8 37.0012841209275 39.0 37.0 40.0 33.0 40.0 9 36.956193131787494 39.0 37.0 40.0 33.0 40.0 10 36.71932785441738 39.0 37.0 40.0 32.0 40.0 11 35.43797695920164 38.0 35.0 39.0 29.0 40.0 12 33.46081596712651 38.0 31.0 39.0 19.0 40.0 13 32.8411358966833 37.0 30.0 39.0 17.0 40.0 14 32.44208614616965 37.0 29.0 39.0 16.0 40.0 15 32.88499779864984 37.0 30.0 39.0 18.0 40.0 16 32.55725344878192 36.0 30.0 39.0 18.0 40.0 17 32.57046154975051 36.0 30.0 39.0 18.0 40.0 18 32.62477986498386 36.0 30.0 39.0 19.0 40.0 19 33.59768491341356 36.0 31.0 39.0 24.0 40.0 20 33.8632594658057 37.0 31.0 39.0 24.0 40.0 21 35.14305107132375 38.0 35.0 39.0 26.0 40.0 22 34.7885786615791 38.0 34.0 39.0 25.0 40.0 23 35.10806794834165 38.0 35.0 39.0 27.0 40.0 24 34.67671338420898 38.0 33.0 39.0 25.0 40.0 25 34.973547108893456 38.0 34.0 39.0 27.0 40.0 26 35.36443351922512 38.0 35.0 39.0 28.0 40.0 27 35.35913193425301 38.0 35.0 39.0 28.0 40.0 28 35.38171411799237 38.0 35.0 39.0 28.0 40.0 29 35.135823304960375 38.0 35.0 39.0 27.0 40.0 30 35.05453845024949 38.0 35.0 39.0 27.0 40.0 31 34.86786395656002 38.0 35.0 39.0 27.0 40.0 32 33.77843410625184 38.0 33.0 39.0 19.0 40.0 33 35.002861755209864 38.0 35.0 39.0 27.0 40.0 34 34.8054740240681 38.0 35.0 39.0 26.0 40.0 35 34.84009025535662 38.0 35.0 39.0 27.0 40.0 36 32.515060904021134 36.0 31.0 38.0 21.0 39.0 37 32.29855811564426 35.0 31.0 38.0 20.0 39.0 38 31.42526416201937 35.0 30.0 38.0 12.0 39.0 39 29.32875330202524 34.0 27.0 38.0 4.0 39.0 40 25.18828881714118 31.0 11.0 38.0 4.0 39.0 41 21.51258438508952 25.0 8.0 33.0 4.0 37.0 42 25.544540651599647 30.0 20.0 34.0 4.0 37.0 43 21.7465695626651 26.0 8.0 33.0 4.0 37.0 44 22.72308849427649 28.0 9.0 33.0 4.0 37.0 45 25.259245670678016 30.0 19.0 34.0 4.0 38.0 46 25.35544467273261 30.0 12.0 36.0 4.0 38.0 47 24.49345098326974 30.0 7.0 36.0 4.0 38.0 48 24.452854417375992 30.0 7.0 36.0 4.0 38.0 49 21.825157763428237 26.0 4.0 35.0 4.0 38.0 50 24.272362048723217 30.0 4.0 35.0 4.0 38.0 51 25.69592016436748 32.0 4.0 38.0 4.0 40.0 52 25.441884355738186 32.0 4.0 38.0 4.0 40.0 53 25.056923246257703 32.0 4.0 38.0 4.0 40.0 54 24.12696287056061 31.0 4.0 38.0 4.0 40.0 55 24.33326606985618 31.0 4.0 38.0 4.0 40.0 56 23.360324332257118 30.0 4.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.01643988558426912 1 2 2.3623136379792626 1 3 0.6003199312788254 1 4 0.4335301171061374 1 5 0.27174842415684 1 6 0.6543938701578966 1 7 0.855012573575074 1 8 1.1098197620488506 1 9 0.8390426779508147 1 10 1.2460142658548463 1 11 -4.1866226060193235 1 12 -8.226683826857915 1 13 -21.49000765958504 1 14 -19.3792814188175 1 15 -13.060600908933374 1 16 -21.71735441366327 1 17 -21.307381727219756 1 18 -20.68607519417489 1 19 -6.375535973437305 1 20 -11.812279795466683 1 21 -9.13165574159066 1 22 -4.04422986223199 1 23 -5.148468868805349 1 24 -4.469110255849301 1 25 -5.117489002345788 1 26 1.6493799184995055 1 27 1.0831062699646665 1 28 1.4559430154212052 1 29 1.00459411645474 1 30 1.9066142713538383 1 31 0.5946851489140244 1 32 -10.330529888502223 1 33 0.3893016576894368 1 34 0.541394658665844 1 35 0.2667458225231769 1 36 3.6154497824218907 1 37 4.174550066341833 1 38 4.968104869661673 1 39 7.3632267569660215 1 40 10.091997823378225 1 41 -2.6662116015643385 1 42 -7.977259908485637 1 43 -12.967495146103028 1 44 -15.456678089374485 1 45 -14.757018672755306 1 46 -18.61153045440893 1 47 -17.68372436993844 1 48 -16.727630186345948 1 49 -14.645610159663082 1 50 -16.538131983105764 1 51 -17.699523030783965 1 52 -17.551844699040686 1 53 -17.324692089770366 1 54 -17.41885163352587 1 55 -16.73844745461627 1 56 -16.383133403725328 2 1 0.7631617627409284 2 2 2.704618675936473 2 3 1.0544262114720624 2 4 1.1747309452634909 2 5 0.930133517324542 2 6 1.340384208322149 2 7 1.7390705445895662 2 8 1.7854581333393966 2 9 1.7320723535891887 2 10 1.8022599525353158 2 11 -2.410225090491373 2 12 -11.058981963503879 2 13 -12.56040103432624 2 14 -15.03352572523572 2 15 -8.22623237891267 2 16 -2.9092108663892873 2 17 -1.4681822793246582 2 18 -9.353431991966474 2 19 -2.533576001042551 2 20 -3.1732183737965656 2 21 2.0905664806315656 2 22 1.909531352398787 2 23 1.8881080808150799 2 24 2.163739985696594 2 25 2.147521349621087 2 26 1.6790555568708 2 27 1.5482546481565223 2 28 1.5449699305350748 2 29 1.6567680294982168 2 30 2.3724527806706064 2 31 -1.814562608159818 2 32 -9.86607164143528 2 33 -1.9260882663961425 2 34 -1.4268593095881243 2 35 -1.2494722589122986 2 36 3.988120589875301 2 37 4.64383923128317 2 38 5.270382302373889 2 39 7.252115645854907 2 40 10.628229707436194 2 41 6.836893988497774 2 42 2.872291506283169 2 43 0.27957179661608933 2 44 -9.116443444792013 2 45 1.6950172140631885 2 46 -11.142931420592506 2 47 -9.933551837157209 2 48 -7.675180220852507 2 49 -12.109378275605113 2 50 -7.745171320579882 2 51 -8.54355339655346 2 52 -9.005950979233921 2 53 -8.65664516085387 2 54 -15.221474131800543 2 55 -8.031753182704607 2 56 -7.33344396273154 3 1 1.1413758814880097 3 2 3.3525850153474153 3 3 1.4784561319956708 3 4 1.6025009312382466 3 5 1.348553339671433 3 6 1.8658634042080777 3 7 1.8335071972309862 3 8 2.1968653329653876 3 9 2.3239377112329436 3 10 1.8578934261211018 3 11 1.8010886074983432 3 12 2.3739306124662 3 13 -3.5791013615819693 3 14 -5.709542555530248 3 15 -1.320201523372699 3 16 3.6354361649337648 3 17 3.8380355794878724 3 18 3.23609582477032 3 19 2.0919499456615185 3 20 1.7734856935246128 3 21 3.2626094913842536 3 22 3.4724112027961738 3 23 2.9227036862381723 3 24 3.4592051563370774 3 25 2.9731407979614346 3 26 2.6734454586940757 3 27 2.593602941751662 3 28 2.420612754284491 3 29 2.7680349766697887 3 30 2.35515497795906 3 31 2.979733792027183 3 32 2.306438877498806 3 33 2.3590917242536946 3 34 3.0032482172935957 3 35 2.093678746230289 3 36 4.686110304695312 3 37 5.356321699726369 3 38 6.315263087787635 3 39 9.101578011446307 3 40 11.651137608324458 3 41 11.877567200278978 3 42 9.06677490974273 3 43 3.852269318822728 3 44 4.923762258807798 3 45 8.382254240711156 3 46 1.5569283269530771 3 47 -2.7180305655349564 3 48 2.445436889946933 3 49 -9.548835966114696 3 50 1.0594102595977688 3 51 -1.0026464306815548 3 52 -0.4514862761016154 3 53 -1.2012921921769184 3 54 -7.235031869060945 3 55 -0.9125385964493482 3 56 2.2420586085634575 4 1 1.3116066414251861 4 2 3.29133093778308 4 3 1.6614934643624721 4 4 1.58655312406173 4 5 1.4611545236752974 4 6 1.8841067290842375 4 7 1.881229802039492 4 8 2.0163651517403025 4 9 2.3373483672677366 4 10 2.4143752432045886 4 11 3.485394515435999 4 12 4.640089848904527 4 13 5.385861789318113 4 14 1.3478453869609943 4 15 6.038382504656784 4 16 6.447082896841458 4 17 6.42580892731921 4 18 5.7498085226077045 4 19 4.5504494019862705 4 20 4.776626928271504 4 21 3.2628511248263194 4 22 3.7517394618272206 4 23 3.682278411380132 4 24 3.3985551623779173 4 25 3.300916562127192 4 26 2.2771666137019295 4 27 2.8255710461373056 4 28 1.8074678950359697 4 29 3.0869911202000537 4 30 2.4828582520922 4 31 3.3696093508045237 4 32 3.1774066194342865 4 33 2.962692062540512 4 34 3.026324211011122 4 35 3.412634889760554 4 36 5.668954330308459 4 37 5.837293066163479 4 38 7.740175495664886 4 39 9.079831001660153 4 40 10.826080220381971 4 41 7.368566354561935 4 42 9.326289226524171 4 43 9.416845856215502 4 44 12.356769386994344 4 45 10.20356631030159 4 46 8.179980157326401 4 47 8.832772865659923 4 48 8.853072506716295 4 49 4.867498454569127 4 50 8.777544849425002 4 51 7.221468586836867 4 52 7.364509857763313 4 53 7.762341974791788 4 54 2.959603868525136 4 55 8.459697721056994 4 56 9.001029250100245 5 1 1.5667044358488198 5 2 3.9284512041168256 5 3 1.6743940053529016 5 4 2.204253221162574 5 5 2.2258930449681245 5 6 2.1729123886764157 5 7 2.4846422032047 5 8 2.7747227602851794 5 9 2.9236987626068967 5 10 2.4453087984827064 5 11 3.501208081811363 5 12 6.633986367145614 5 13 6.7375055679281886 5 14 6.417896711693967 5 15 7.084019902354104 5 16 7.150370454061601 5 17 6.948350195178833 5 18 6.719568544802183 5 19 5.307003709102375 5 20 5.080136430282877 5 21 3.8189615423599577 5 22 4.479579661577532 5 23 4.031425311088832 5 24 4.049408262669218 5 25 4.214080662557208 5 26 3.5400324758187267 5 27 2.963176816701882 5 28 3.5599994528109704 5 29 3.6836064621337528 5 30 3.2822756470152115 5 31 4.005619893182107 5 32 4.658888100915767 5 33 3.7808360492238293 5 34 3.7459801965847106 5 35 3.5154230712004235 5 36 5.493756660728764 5 37 6.003474228599327 5 38 6.530715098938572 5 39 9.054584781657375 5 40 7.968541033254763 5 41 3.7306137952610605 5 42 10.514803583577855 5 43 14.145379633438417 5 44 13.412810446780718 5 45 11.521640939237642 5 46 12.363778187566378 5 47 12.517333831119764 5 48 11.974309848927419 5 49 13.230933050213459 5 50 12.611779833472724 5 51 10.983016651755719 5 52 10.867908387379071 5 53 11.975131543386603 5 54 11.591730377056138 5 55 11.84532679582465 5 56 10.287060599802007 6 1 1.1607034982023166 6 2 3.712323684191837 6 3 1.814103334935652 6 4 1.8560738181308523 6 5 2.0203921854588245 6 6 1.9188101852071213 6 7 2.2052235440392067 6 8 2.067424744970289 6 9 2.0413733992688776 6 10 1.5837679327224379 6 11 3.7524973457588544 6 12 6.265178740954017 6 13 5.789076125827862 6 14 6.510411167077617 6 15 6.713962080819488 6 16 6.5193343546710665 6 17 6.4024836535316965 6 18 6.236368044724042 6 19 5.336539574081279 6 20 5.292200815343044 6 21 3.542043273880509 6 22 4.080142249481895 6 23 3.2658369379055188 6 24 3.5491738510424042 6 25 2.930130045273259 6 26 2.857738367364284 6 27 2.685477176133041 6 28 2.781440302943807 6 29 2.658602555273305 6 30 3.2067951031802338 6 31 3.1436102041681977 6 32 5.1774066194342865 6 33 3.2074652100302004 6 34 3.4423390026481613 6 35 3.705765312175572 6 36 3.5171978234104344 6 37 3.4110379104246142 6 38 4.667924037835277 6 39 5.93987935893508 6 40 3.5434746166271687 6 41 -0.7813411977065918 6 42 7.06473326008981 6 43 12.629048956770191 6 44 11.048847327543339 6 45 7.886541704982289 6 46 10.589594721399788 6 47 11.15633992582206 6 48 9.951493783917257 6 49 12.004178869872781 6 50 10.853067534676036 6 51 10.16585772067274 6 52 10.247030125150598 6 53 10.484429871250327 6 54 10.55141157724367 6 55 9.688114731439587 6 56 10.487873226774973 7 1 1.677815546959934 7 2 3.4716610806600343 7 3 1.760813758439319 7 4 1.9449939619033145 7 5 1.7197202054619538 7 6 1.7778506602813522 7 7 2.1883459069084026 7 8 2.28089559979135 7 9 2.4422172811254157 7 10 2.013210033050612 7 11 3.3654056126755663 7 12 5.967319700478942 7 13 6.441209271631891 7 14 6.874686835150758 7 15 6.800069285070155 7 16 6.557777861469006 7 17 6.454523034685003 7 18 5.707222865789834 7 19 5.195892597991268 7 20 4.956679640159422 7 21 3.6831590732241537 7 22 3.4055055875034554 7 23 2.8338944468913 7 24 3.543235423163047 7 25 3.337537452680664 7 26 2.478304080756999 7 27 2.481695335220394 7 28 2.572345131823319 7 29 2.387310165837455 7 30 2.4921521902250845 7 31 2.3883359425648223 7 32 3.436665878693546 7 33 1.5833051850262976 7 34 2.029930813868667 7 35 1.8117193674967211 7 36 0.6542504878892572 7 37 1.1763137347721688 7 38 1.765283000173138 7 39 3.6842144112870088 7 40 -0.537631806251408 7 41 -9.689139291158693 7 42 5.15677889221983 7 43 10.021922843314961 7 44 8.375773409743683 7 45 5.595715013311715 7 46 7.240321397442923 7 47 8.38153136198396 7 48 8.295297503248406 7 49 10.66303181564556 7 50 7.438940327299882 7 51 8.785485787558187 7 52 8.559266412070428 7 53 8.864020432275492 7 54 10.036174821500584 7 55 9.450265067429587 7 56 8.533974180048922 8 1 0.8437414728858599 8 2 3.1388215744871957 8 3 1.7158754868343848 8 4 1.410672974248996 8 5 1.523176995585409 8 6 1.663282759046787 8 7 1.6416792402417357 8 8 2.139661031890121 8 9 2.0555506144587525 8 10 2.256172996013575 8 11 3.2686154892187744 8 12 5.96188760171351 8 13 6.180468530891147 8 14 6.523575724039642 8 15 6.381303852971389 8 16 6.476296379987524 8 17 6.810078590240558 8 18 6.312654964555268 8 19 4.800337042435707 8 20 4.723593220406336 8 21 3.3038998139648967 8 22 3.977357439355309 8 23 3.665499385162903 8 24 3.722000855261811 8 25 3.4367967119399268 8 26 2.930649759769345 8 27 2.8456459525043485 8 28 2.3797525392307293 8 29 2.7779274497880735 8 30 3.27042379516336 8 31 3.281669275898153 8 32 3.750739952767617 8 33 3.2356508640386394 8 34 3.4264740237452074 8 35 3.3835712193485676 8 36 1.9144974014695038 8 37 2.291375463167224 8 38 3.1376286791854824 8 39 4.572115645854911 8 40 0.4386644900448893 8 41 -9.833830649183385 8 42 6.905914694688967 8 43 10.90044136183348 8 44 9.062686989990596 8 45 7.274727358990727 8 46 9.970691767813289 8 47 10.74992642371236 8 48 10.648877750161986 8 49 13.407723173670249 8 50 10.294248969275188 8 51 11.307461096200164 8 52 11.372599745403758 8 53 11.051180926102653 8 54 12.250989636315403 8 55 11.335203339034525 8 56 10.548295167703241 9 1 1.2619693209871272 9 2 2.8135895069766477 9 3 1.7128375121508412 9 4 1.7674662231941412 9 5 1.640645350015788 9 6 1.412481071283075 9 7 1.559653923786044 9 8 1.7256525930715512 9 9 2.054031627116977 9 10 1.4825021099376272 9 11 3.4233834217082233 9 12 5.5816344371565485 9 13 5.789076125827862 9 14 6.687626356951039 9 15 5.372189928920758 9 16 6.240853342012841 9 17 6.693622894038029 9 18 5.198393361179736 9 19 4.956792738638242 9 20 3.9377704355962067 9 21 3.453435678943798 9 22 3.320648578595815 9 23 2.7215331404371668 9 24 3.7517054966120185 9 25 2.626332576918827 9 26 1.9969788736934007 9 27 2.9133252773988616 9 28 2.5535922016779864 9 29 2.6712607831214044 9 30 2.4219849765979617 9 31 2.763863368725154 9 32 2.9622167460165656 9 33 2.397338627751722 9 34 2.4043643191038555 9 35 1.8196893628084894 9 36 1.6437801018914442 9 37 2.1325568977663814 9 38 3.5413417593542604 9 39 4.382917333618622 9 40 -1.0641203200816918 9 41 -3.0218475268205154 9 42 6.950809209456899 9 43 11.477150222592975 9 44 10.567834669315491 9 45 6.557427780931654 9 46 9.855417506209914 9 47 10.548744989113203 9 48 10.799595049740049 9 49 13.206710515442403 9 50 10.637877661258315 9 51 10.381047594090461 9 52 10.044498479580977 9 53 9.446455187706029 9 54 9.753943222813291 9 55 9.257734984604145 9 56 8.918252973610414 10 1 1.902442771587154 10 2 3.939514215179841 10 3 2.2208105517694463 10 4 1.957339640915663 10 5 1.9600601124685255 10 6 2.2099494257134538 10 7 2.209990928553424 10 8 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-7.8113499811880125 117 18 -3.4797259878256845 117 19 -3.216805814707147 117 20 -3.582121065307952 117 21 1.1500902901553758 117 22 0.6145002964981643 117 23 0.5935946232581415 117 24 -0.26276104950009227 117 25 0.5015586167018284 117 26 1.9906497597693473 117 27 1.2894554763138686 117 28 -0.11548555600737131 117 29 0.20697506883568906 117 30 0.8708999856395465 117 31 -2.6949973907685134 117 32 -10.25116480913714 117 33 0.6075556259434052 117 34 1.0612359285071093 117 35 -0.2530954473180955 117 36 1.6392593062314091 117 37 2.1904230822148456 117 38 3.182390583947388 117 39 4.01402040775967 117 40 2.7943787757591743 117 41 3.8456931603404243 117 42 1.006867075641349 117 43 -7.638130066737949 117 44 -5.35350348619988 117 45 -1.3919393076759405 117 46 -7.484546327424804 117 47 -12.651978338192405 117 48 -9.01731272602849 117 49 -7.56227682632975 117 50 -6.919084364058143 117 51 -5.382062713323645 117 52 -5.285971683167666 117 53 -5.856438121516398 117 54 -9.172819887494125 117 55 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4.904708796500561 118 38 6.137277801992504 118 39 8.559133189714558 118 40 10.627085542676472 118 41 8.163362333272755 118 42 0.9767918876714248 118 43 0.43517820393874374 118 44 -0.02643581702694675 118 45 3.7396396396924807 118 46 -4.898080162011269 118 47 -8.644459541199923 118 48 -9.150771372645032 118 49 -6.733329457908697 118 50 -3.7611896272160372 118 51 -3.7918371494138725 118 52 -4.081084465122554 118 53 -6.036889249335946 118 54 -9.184098082982846 118 55 -6.313217713597055 118 56 -2.855564481419563 119 1 0.421923291067678 119 2 2.3594276350932546 119 3 0.7619360928949916 119 4 0.4724911560671785 119 5 0.6613588137672295 119 6 0.664494880258907 119 7 0.673194391756887 119 8 0.8039034561325451 119 9 0.502823341731478 119 10 0.5519305717711518 119 11 -9.42471784411456 119 12 -9.94962754980164 119 13 -0.9661981357755174 119 14 -2.084909124445204 119 15 -5.632029480361943 119 16 -6.772188468497323 119 17 -9.565678985517014 119 18 -0.9212844293841265 119 19 -1.0263296242309572 119 20 -0.6751946583815425 119 21 0.9087482988133786 119 22 0.4088725908704589 119 23 0.8024690821326033 119 24 -4.903453690192734 119 25 0.5361906513338681 119 26 -0.4790472099276215 119 27 1.0383732252316236 119 28 1.3433889028670905 119 29 1.398533510394131 119 30 1.7637571284966924 119 31 -2.0965125422836657 119 32 -14.216532774505108 119 33 -1.19222792384015 119 34 -0.4571623398911555 119 35 -0.8212772654999156 119 36 2.7193458863179885 119 37 1.4198603116520765 119 38 2.0806589822157875 119 39 5.615752009491274 119 40 7.910179641560038 119 41 6.316472381119645 119 42 -0.6771156083413352 119 43 -3.1868313654392466 119 44 -0.013676646373042445 119 45 -1.4525453682820029 119 46 -5.813550656429133 119 47 -9.52643721265128 119 48 -9.457788916504681 119 49 -9.009246523299447 119 50 -8.222114667088446 119 51 -8.208902540163471 119 52 -7.834672981868966 119 53 -11.096697861776137 119 54 -10.603555818230054 119 55 -12.21752393369275 119 56 -8.690492711084636 120 1 0.012832381976764395 120 2 1.8518518775174968 120 3 0.40587548683438257 120 4 0.4573396409156629 120 5 0.2295406319490425 120 6 0.23267669844072714 120 7 0.47622469478719154 120 8 0.735721637950725 120 9 0.2755506144587514 120 10 0.43071845055902713 120 11 -6.674717844114561 120 12 -1.8208396710137613 120 13 -4.663167832745213 120 14 -7.25915154868763 120 15 -8.094150692483158 120 16 -8.946430892739748 120 17 -10.285375955213986 120 18 -2.4440117021114 120 19 -2.677844775746106 120 20 -3.5691340523209405 120 21 -0.47003957997449675 120 22 -0.15173346973559632 120 23 -1.0005612208977013 120 24 -5.759514296253339 120 25 -1.069869954726741 120 26 0.24064975976934733 120 27 0.6141308009891944 120 28 0.49490405438223917 120 29 0.648533510394131 120 30 0.10466621940578591 120 31 -3.126815572586697 120 32 -13.322593380565714 120 33 -2.798288529900752 120 34 -11.032919915648733 120 35 -3.639459083681732 120 36 -2.0458056288335236 120 37 -2.2998366580448923 120 38 -1.3435834420266346 120 39 0.3581762519155163 120 40 -5.764062782682384 120 41 -2.592618527971265 120 42 -5.775600456826183 120 43 -8.777740456348338 120 44 -8.415191797888193 120 45 -11.369212034948667 120 46 -13.987793080671556 120 47 -14.405225091439158 120 48 -11.510819219534984 120 49 -14.925913189966113 120 50 -11.80544800042178 120 51 -11.693751025011958 120 52 -11.152854800050784 120 53 -13.369425134503409 120 54 -13.861131575805814 120 55 -12.922069388238203 120 56 -9.89503816563009 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 433.0 5 28.0 6 45.0 7 51.0 8 117.0 9 150.0 10 113.0 11 78.0 12 93.0 13 91.0 14 128.0 15 184.0 16 269.0 17 346.0 18 410.0 19 491.0 20 712.0 21 850.0 22 1092.0 23 1298.0 24 1471.0 25 1654.0 26 1770.0 27 1849.0 28 1968.0 29 2030.0 30 2250.0 31 2570.0 32 2963.0 33 3509.0 34 4330.0 35 5189.0 36 5865.0 37 5768.0 38 3319.0 39 1028.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 0.0 100.0 7 99.19283827414147 0.5980334605224538 0.15592896976812445 0.053199295567948335 8 98.88464925154095 0.8346786028764309 0.13208100968594072 0.1485911358966833 9 93.9463446858485 3.644304169113329 1.18357310628303 1.225778038755138 10 37.61190196653948 39.4078368065747 9.799677135309656 13.180584091576167 11 26.340121078701156 32.711429095578794 19.502843514951383 21.445606310768667 12 31.802171998825944 16.335852656295863 23.690196653947755 28.171778690930438 13 24.003889051951862 15.50300851188729 27.65813031992956 32.834972116231285 14 22.268097990641344 19.94678410863382 23.87374988531058 33.911368015414254 15 24.822695035460992 18.944989527064273 25.10381067871973 31.128504758755003 16 25.111037697757222 18.435928495393313 27.663987079249715 28.78904672759975 17 26.959489211800967 24.209232349919272 22.220387494495817 26.610890943783943 18 28.094443119484858 26.782733126639634 20.271881707607918 24.850942046267587 19 28.54049689896877 26.9092443759404 18.976843186905942 25.573415538184886 20 27.221008973959005 25.56018425061019 22.882677873410287 24.33612890202052 21 25.806333247715845 25.93475947602099 23.369904230726892 24.88900304553627 22 25.28297040964209 25.196749279961843 22.527563244115868 26.992717066280203 23 28.531332550631056 26.058482535955385 23.83878778984444 21.57139712356912 24 22.912386263574994 26.84913413560317 22.850014675667744 27.388464925154093 25 24.965145289110655 27.48202230701497 23.688362195479897 23.86447020839448 26 21.905497050228814 26.865891088199078 27.740714193821102 23.487897667751007 27 22.02605982749128 22.94916498440081 29.45678106074509 25.56799412736282 28 22.447217066941068 23.904375149298986 31.388618364234393 22.25978941952555 29 20.20038903365508 23.531030939185964 34.471684956141964 21.79689507101699 30 19.28752340050655 24.839408288367654 35.91748339022868 19.955584920897113 31 19.80547537185828 24.299031054073435 35.595432900034936 20.30006067403335 32 20.12072065460683 21.797600264191097 37.57384508127545 20.507833999926614 33 21.109518032425278 23.451343700599242 38.37358920627918 17.0655490606963 34 18.080496492967573 26.286217913407512 40.63934486430906 14.993940729315852 35 20.151993538438944 25.435053968720172 39.68353036199427 14.729422130846611 36 17.041174417109158 25.259521928454625 41.02375659139766 16.675547063038564 37 16.926613941511864 26.091594629391206 39.65422107780026 17.32757035129667 38 20.116494864303693 26.59169836282454 39.619278613821365 13.672528159050401 39 18.09311658903883 21.760341685229847 35.598961689279996 24.547580036451333 40 10.801637953296197 18.063599808167634 29.438890323532668 41.69587191500351 41 13.768156166157834 19.800166527893424 31.157368859283928 35.27430844666482 42 14.992738036144356 28.352913058665635 41.406063280201494 15.248285624988508 43 9.962073790411665 15.078429928566168 31.626040209146478 43.333456071875695 44 11.28681484758008 17.811198348630615 29.250986029709903 41.6510007740794 45 15.83481823024781 25.62962690816908 41.367084887117215 17.168469974465896 46 16.872911541144934 25.904233833951455 41.780193148018945 15.44266147688466 47 14.614452967299151 23.56589716299042 38.19136051675414 23.628289352956287 48 16.35839847333847 28.07809460897648 41.10059084737054 14.462916070314508 49 11.486871883567314 17.74641667739977 29.172569872490755 41.59414156654216 50 15.398474124459968 27.993381744645646 41.60308851916537 15.005055611729018 51 17.76161988034975 27.688909341923605 39.35757017947538 15.191900598251266 52 15.328520919269298 30.04751031231585 39.127872716558635 15.496096051856217 53 15.403989337255261 29.655299200294145 40.37871127861017 14.562000183840427 54 15.307340544709922 25.529375034434633 35.19678242823823 23.966501992617214 55 15.605704204645694 28.829755954131137 40.42193472508086 15.14260511614231 56 16.38973642612947 28.807484468624782 38.63912068521854 16.1636584200272 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 1.0 5 2.0 6 6.0 7 10.0 8 13.0 9 16.0 10 48.0 11 80.0 12 133.0 13 211.0 14 236.0 15 273.0 16 310.0 17 387.0 18 464.0 19 510.5 20 557.0 21 642.0 22 940.0 23 1153.0 24 1434.5 25 1716.0 26 1984.0 27 2252.0 28 2443.5 29 2810.0 30 2985.0 31 2838.5 32 2692.0 33 2512.5 34 2333.0 35 2062.0 36 1791.0 37 1638.5 38 1403.5 39 1321.0 40 1308.0 41 1295.0 42 1242.0 43 1189.0 44 1199.5 45 1210.0 46 1252.0 47 1350.5 48 1407.0 49 1439.5 50 1472.0 51 1566.5 52 1661.0 53 1865.0 54 2272.5 55 2476.0 56 2662.5 57 2849.0 58 2904.5 59 2960.0 60 2810.0 61 2660.0 62 2443.5 63 2119.0 64 2011.0 65 1750.5 66 1490.0 67 1292.0 68 1094.0 69 949.0 70 804.0 71 707.0 72 499.5 73 389.0 74 301.0 75 213.0 76 161.5 77 110.0 78 79.0 79 29.5 80 11.0 81 10.5 82 10.0 83 5.0 84 0.0 85 0.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0293513354857646 10 0.0 11 0.003668916935720575 12 0.0 13 0.0 14 0.03118579395362489 15 0.15776342823598474 16 0.04769592016436748 17 0.0146756677428823 18 0.005503375403580863 19 0.025682418550044026 20 0.03852362782506604 21 0.011006750807161726 22 0.0018344584678602876 23 0.0 24 0.0 25 0.0 26 0.18528030525388905 27 0.04035808629292632 28 0.1669357205752862 29 0.03302025242148518 30 0.04769592016436748 31 0.2256383915468154 32 0.011006750807161726 33 0.20179043146463163 34 0.09172292339301438 35 0.06604050484297036 36 0.15592896976812445 37 0.26049310243616086 38 0.16326680363956558 39 0.3540504842970355 40 0.5466686234223657 41 0.8566921044907543 42 0.21830055767537423 43 0.3595538597006164 44 0.46595245083651304 45 0.13758438508952156 46 0.08438508952157323 47 0.03302025242148518 48 0.025682418550044026 49 0.2990167302612269 50 0.21463164073965368 51 0.34304373348987377 52 0.38156736131493985 53 0.21463164073965368 54 0.11190196653947755 55 0.1761080129145876 56 0.19445259759319047 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 54512.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.25168770179043 #Duplication Level Percentage of deduplicated Percentage of total 1 94.8006016504736 85.55914294100381 2 3.609902841578926 6.5159964778397415 3 0.7419000772389122 2.008732022307015 4 0.32521647221431765 1.174053419430584 5 0.16057563315581935 0.7246110948048137 6 0.10163014756697426 0.5503375403580862 7 0.05691288263750559 0.3595538597006164 8 0.03252164722143176 0.2348106838861168 9 0.03455425017277125 0.280672145582624 >10 0.12805398593438758 2.1444819489286764 >50 0.00813041180535794 0.4476078661579102 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TACTTCGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC 74 0.1357499266216613 No Hit TACTTCGGGAATTGACGGGTACGCACAGTTCTGAGTCGGCCACACGCAGGGATGAG 58 0.10639859113589667 No Hit TACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA 58 0.10639859113589667 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.00733783387144115 0.0 0.0 0.0 0.0 11 0.01651012621074259 0.0 0.0 0.0 0.0 12 0.02201350161432345 0.0 0.0 0.0 0.0 13 0.025682418550044026 0.0 0.0 0.0 0.0 14 0.03118579395362489 0.0 0.0 0.0 0.0 15 0.03485471088934546 0.0 0.0 0.0 0.0 16 0.03485471088934546 0.0 0.0 0.0 0.0 17 0.04035808629292633 0.0 0.0 0.0 0.0 18 0.04953037863222776 0.0 0.0 0.0 0.0 19 0.05503375403580863 0.0 0.0 0.0 0.0 20 0.06053712943938949 0.0 0.0 0.0 0.0 21 0.06420604637511007 0.0 0.0 0.0 0.0 22 0.06787496331083065 0.0 0.0 0.0 0.0 23 0.08071617258585266 0.0 0.0 0.0 0.0 24 0.09539184032873496 0.0 0.0 0.0 0.0 25 0.09906075726445553 0.0 0.0 0.0 0.0 26 0.11190196653947755 0.0 0.0 0.0 0.0 27 0.11373642500733784 0.0 0.0 0.0 0.0 28 0.12657763428235985 0.0 0.0 0.0 0.0 29 0.12657763428235985 0.0 0.0 0.0 0.0 30 0.12657763428235985 0.0 0.0 0.0 0.0 31 0.13024655121808043 0.0 0.0 0.0 0.0 32 0.13208100968594072 0.0 0.0 0.0 0.0 33 0.13208100968594072 0.0 0.0 0.0 0.0 34 0.13208100968594072 0.0 0.0 0.0 0.0 35 0.13208100968594072 0.0 0.0 0.0 0.0 36 0.13208100968594072 0.0 0.0 0.0 0.0 37 0.13208100968594072 0.0 0.0 0.0 0.0 38 0.13208100968594072 0.0 0.0 0.0 0.0 39 0.133915468153801 0.0 0.0 0.0 0.0 40 0.133915468153801 0.0 0.0 0.0 0.0 41 0.1357499266216613 0.0 0.0 0.0 0.0 42 0.14125330202524214 0.0 0.0 0.0 0.0 43 0.14125330202524214 0.0 0.0 0.0 0.0 44 0.146756677428823 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGATAGA 15 0.008066127 50.49674 40 GCGCTCC 15 0.008066127 50.49674 38 GGCCAAC 20 3.9505758E-4 50.49674 38 AGACCCT 15 0.008066127 50.49674 40 AGACCCG 15 0.008066127 50.49674 40 AGCGATA 15 0.008066127 50.49674 38 AGCGCTC 25 1.954441E-5 50.449722 37 TGCCCAG 15 0.008095923 50.44972 41 CCACAGT 15 0.008095923 50.44972 41 TTTGTTC 15 0.008095923 50.44972 37 TATCTTT 20 4.0239142E-4 50.309193 46 GCACGGA 15 0.008185802 50.309193 46 TACGAAG 15 0.008185802 50.309193 47 CGCCGGA 15 0.008185802 50.309193 47 TTATCTT 15 0.008215926 50.262524 45 CGCACGG 15 0.008215926 50.262524 45 CTGCGCA 15 0.008246132 50.215942 44 ATTCTCT 15 0.008246132 50.215942 44 ATTTTCT 15 0.008246132 50.215942 44 TTGCCTA 15 0.008246132 50.215942 42 >>END_MODULE