FastQCFastQC Report
Fri 17 Jun 2016
SRR1526325_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526325_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76142
Sequences flagged as poor quality0
Sequence length56
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG5380.7065745580625673No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG4470.5870610175724305No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG3720.4885608468388012No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG3160.41501405269102465No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG3100.40713403903233436No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC2390.31388721073783193No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAACGATTAAG2270.29812718342045125No Hit
ACAATAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC1750.22983373171180163No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1700.223267053662893No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGCCTCAAAGATTAAG1050.13790023902708098No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTCAG930.1221402117097003No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG900.11820020488035513No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTCCCTACCAAGCGC830.10900685561188307No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAAAGATTAAG820.10769352000210135No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG790.10375351317275616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACCT451.1823431E-1050.5644640
AACAACC251.9357416E-550.5644638
CCGGAAC150.0080358550.5644640
TTCGTTT150.0080358550.5644638
GCGAACC150.0080358550.5644639
CGCTAAT203.9345934E-450.5644640
TGCTTTT251.9357416E-550.5644639
GAACTCT150.0080358550.5644639
GCGCTAA203.9345934E-450.5644639
CTACGCT150.0080358550.5644640
CAAACCA505.456968E-1250.5644639
CCTGTGC354.811045E-850.49699841
TACGCTC150.00807841250.49699841
TGCTCGT505.456968E-1250.4297137
CGGCTAA150.00812114150.4297137
AGCGCTC203.9868115E-450.4297137
CAGAACT203.9868115E-450.4297137
TTTGCTT203.9868115E-450.4297137
ATGCTTG2200.050.39613736
TCAGAAC309.799205E-750.39613736