FastQCFastQC Report
Fri 17 Jun 2016
SRR1526324_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526324_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39604
Sequences flagged as poor quality0
Sequence length56
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAGGACCACTGACTGCTCTTCG1020.2575497424502575No Hit
AGCGAGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAGGAGCACTGACTGCTCTTCG950.23987476012523987No Hit
AGCGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC660.16664983335016667No Hit
AGCGAGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAGGAGCACCGACTCCTCTTCG510.12877487122512876No Hit
AGCGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC490.12372487627512373No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC470.11867488132511868No Hit
AGCGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC410.10352489647510353No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTCG150.00814055950.3541439
ACGCACC150.00814055950.3541438
AACAGTC150.00814055950.3541438
CCCCCAA150.00814055950.3541440
AACAAAC150.00818170350.29007737
CACCGTT150.00818170350.29007741
GAACAGT150.00818170350.29007737
CTATTGT150.00818170350.29007741
TAGGACC251.9965739E-550.22617735
TCGAACA204.04339E-450.22617735
CGAACAG204.04339E-450.22617736
CTCGAAC204.1202045E-450.03544634
CAATGTG150.00834782750.03544615
CCAACAC204.1202045E-450.03544643
GAGTGAC150.00834782750.03544610
ACCAATG150.00834782750.03544613
TATTTTC252.0421616E-550.03544643
TTAGGAC204.1202045E-450.03544634
ATTTTCT301.0171789E-650.03544644
GACCAAT150.00834782750.03544612