FastQCFastQC Report
Fri 17 Jun 2016
SRR1526323_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526323_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35592
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG950.2669139132389301No Hit
TTGGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG860.24162733198471567No Hit
TTGGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG710.1994830298943583No Hit
TTGGACGGGTACGCACAGTTCTGAGTCGGAGACACGCAGGCATGAGATGGGCAAAA650.18262530905821533No Hit
TTGGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG550.15452910766464373No Hit
TTGGACGGGTACGCACAGTTCTGAGTCGGAGACACGCAGCCATGAGATGGCCAAAA540.15171948752528658No Hit
TTGGACGGGTACGCACAGTTCTGAGTCGGAGACACGCAGGCATGAGATGGCCAAAA530.14890986738592943No Hit
TTGGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG490.13767138682850077No Hit
TTGGACGGGTACGCACAGTTCTGAGTCGGAGACACGCAGCCATGAGATGGGCAAAA450.12643290627107215No Hit
TTGGACGGGTACGCACAGTTCTGAGTCGGAGACACGCAGGCATGAGATGGGGAAAA430.12081366599235784No Hit
TTGGACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC420.11800404585300067No Hit
TTGGACGGGTACGCACAGTTCTGAGTCGGAGACACGCAGCGATGAGATGGCCAAAA390.1095751854349292No Hit
TTGGACGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC360.10114632501685772No Hit
TTGGACGGGTACGCACAGTTCTGAGTCGGAGACACGCAGGGATCAGATCGCCAAAA360.10114632501685772No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATGA251.920792E-550.53637739
CCGTCTC203.9164588E-450.53637740
CCCGTCT203.9164588E-450.53637739
GCTTGTC251.920792E-550.53637738
TTGTCTC309.4653296E-750.53637340
TTCGTTT150.00801800450.53637338
GGATCAG309.4653296E-750.53637340
TCGTTTT150.00801800450.53637339
GACCAGC150.00801800450.53637341
CGTCTCA309.4653296E-750.53637341
TATGCTT451.200533E-1050.32102635
TGCTTGT251.9701109E-550.32102637
ATGCTTG451.200533E-1050.32102636
GCCCCAC150.00815478750.32102237
TTTTCGT150.00815478750.32102236
TGCTTGC204.0001006E-450.32102237
CAGCTCG204.0282944E-450.24964544
CTCCACG204.0282944E-450.24964544
ACCAACA150.00820076650.24964542
CAACACA204.0282944E-450.24964544