##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526323_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 35592 Sequences flagged as poor quality 0 Sequence length 56 %GC 53 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.77076309282985 39.0 38.0 40.0 34.0 40.0 2 37.64087435378737 39.0 38.0 40.0 34.0 40.0 3 37.0690604630254 39.0 37.0 39.0 32.0 40.0 4 37.066756574511125 39.0 37.0 39.0 33.0 40.0 5 35.51320521465498 39.0 35.0 39.0 23.0 40.0 6 37.35887278040009 39.0 38.0 40.0 33.0 40.0 7 36.9305180939537 38.0 36.0 39.0 33.0 40.0 8 36.863283884018884 38.0 36.0 39.0 32.0 40.0 9 36.90829399865138 39.0 37.0 39.0 32.0 40.0 10 36.78253540121376 39.0 37.0 39.0 32.0 40.0 11 35.54517869184086 38.0 35.0 39.0 29.0 40.0 12 33.6560463025399 38.0 32.0 39.0 21.0 40.0 13 32.863873904248145 37.0 31.0 39.0 17.0 40.0 14 32.67995616992582 37.0 30.0 39.0 17.0 40.0 15 33.03868846931895 37.0 31.0 39.0 18.0 40.0 16 32.9847156664419 37.0 31.0 39.0 19.0 40.0 17 32.824370645088784 36.0 30.0 39.0 19.0 40.0 18 33.11707687120701 36.0 31.0 39.0 21.0 40.0 19 33.97122948977298 37.0 32.0 39.0 25.0 40.0 20 34.223308608676106 37.0 32.0 39.0 26.0 40.0 21 35.44583052371319 38.0 35.0 39.0 28.0 40.0 22 35.10027534277366 38.0 35.0 39.0 27.0 40.0 23 35.21583501910542 38.0 35.0 39.0 27.0 40.0 24 34.77506181164306 38.0 34.0 39.0 26.0 40.0 25 35.17599460552933 38.0 35.0 39.0 27.0 40.0 26 35.320437176893684 38.0 35.0 39.0 28.0 40.0 27 35.353422117329735 38.0 35.0 39.0 28.0 40.0 28 35.281186783546865 38.0 35.0 39.0 28.0 40.0 29 35.17970330411328 38.0 35.0 39.0 28.0 40.0 30 34.83358619914588 38.0 35.0 39.0 27.0 40.0 31 34.69217801753203 38.0 35.0 39.0 26.0 40.0 32 33.428129916835246 38.0 33.0 39.0 19.0 40.0 33 34.88517082490447 38.0 35.0 39.0 27.0 40.0 34 34.621740840638346 38.0 35.0 39.0 26.0 40.0 35 34.65430433805349 38.0 35.0 39.0 27.0 40.0 36 32.77719712294898 36.0 32.0 38.0 23.0 39.0 37 32.53385592267925 36.0 31.0 38.0 23.0 39.0 38 31.622499438075973 35.0 30.0 38.0 17.0 39.0 39 29.837828725556303 35.0 28.0 38.0 7.0 39.0 40 25.283322094852775 31.0 12.0 37.0 4.0 39.0 41 22.111008091706 26.0 9.0 33.0 4.0 37.0 42 25.625421443020905 30.0 20.0 34.0 4.0 37.0 43 21.77722521915037 26.0 9.0 33.0 4.0 36.0 44 22.87626432906271 28.0 10.0 33.0 4.0 36.0 45 25.36246909417847 30.0 20.0 34.0 4.0 37.0 46 25.529472915261856 30.0 17.0 36.0 4.0 38.0 47 24.529669588671613 30.0 10.0 35.0 4.0 38.0 48 24.67706226118229 30.0 11.0 35.0 4.0 38.0 49 22.06827376938638 26.0 5.0 35.0 4.0 38.0 50 24.572432007192628 30.0 10.0 35.0 4.0 38.0 51 26.001461002472467 32.0 4.0 38.0 4.0 39.0 52 25.774359406608227 32.0 4.0 38.0 4.0 39.0 53 25.268065857496065 32.0 4.0 38.0 4.0 39.0 54 24.37224657226343 31.0 4.0 37.0 4.0 39.0 55 24.673887390424813 31.0 4.0 37.0 4.0 39.0 56 24.029360530456284 31.0 4.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.7965355953239097 1 2 1.1503791809851407 1 3 1.9206911522677643 1 4 1.5169955870547156 1 5 3.208779101140216 1 6 1.3816551117235036 1 7 2.048749930401968 1 8 2.2281931847702836 1 9 2.1667705310093623 1 10 1.6719834183502655 1 11 -2.1383809889130276 1 12 -6.298723191274394 1 13 -21.304931712708218 1 14 -16.743021567034365 1 15 -10.329520413062564 1 16 -21.164576669727275 1 17 -21.71689371993839 1 18 -21.243147186416767 1 19 -5.196268083368011 1 20 -11.291257870575304 1 21 -4.541355149030302 1 22 -1.5482453413930557 1 23 -2.0542591804237915 1 24 -2.2518377373730694 1 25 -1.8407982120639161 1 26 2.890346758797328 1 27 2.711918843720923 1 28 2.7050602887230895 1 29 2.940441367442986 1 30 2.2739667021226495 1 31 2.4436933315982543 1 32 -9.430362881093814 1 33 1.2920377019961364 1 34 2.1902378498479607 1 35 2.2941250573684613 1 36 4.441879548626215 1 37 4.941874035951475 1 38 5.5683104561986205 1 39 7.582081326547552 1 40 9.789948995715868 1 41 1.2906813782779238 1 42 -5.888002226045174 1 43 -13.39444856647653 1 44 -15.774365358741957 1 45 -14.513117864982034 1 46 -18.242703471257663 1 47 -17.262066786677217 1 48 -15.819004178263125 1 49 -13.447997966793046 1 50 -15.319943332960744 1 51 -16.6937087197011 1 52 -16.44501279047406 1 53 -17.1581613016051 1 54 -18.022505265472944 1 55 -16.56310955791867 1 56 -16.08418359110197 2 1 0.7659180551059563 2 2 0.8971357974874721 2 3 1.673675065085618 2 4 1.3556048242109213 2 5 2.918171939749456 2 6 1.1865331605039913 2 7 1.8079611395353368 2 8 1.680191109004845 2 9 2.086853561626903 2 10 1.5723466773020007 2 11 -2.900186904844528 2 12 -10.420674410786589 2 13 -13.57581910243318 2 14 -13.97343153070847 2 15 -7.388160786700343 2 16 -1.6119041217251997 2 17 -1.2358350795647546 2 18 -8.159597627516924 2 19 -1.0006271907888724 2 20 -3.132980755889264 2 21 2.586304425437781 2 22 2.583565763952045 2 23 2.4371782871423733 2 24 2.9562577478370997 2 25 2.276482535211642 2 26 1.9136991199805138 2 27 1.3221938826415283 2 28 2.379165114877736 2 29 2.456269078911596 2 30 2.759176873373299 2 31 -2.349768007270452 2 32 -10.402340047673992 2 33 -2.2310551885072343 2 34 -0.8450501626066327 2 35 -0.939398554463402 2 36 4.925532895797989 2 37 4.518936827855985 2 38 6.1900022050310035 2 39 7.280057455245007 2 40 11.457826525555 2 41 8.038994818859138 2 42 2.9604668969439274 2 43 -0.1396483588999864 2 44 -7.71261133694642 2 45 3.253358523186101 2 46 -10.53434851536768 2 47 -11.68137140525532 2 48 -7.659170239498204 2 49 -11.444884318635289 2 50 -7.188651168975275 2 51 -6.662572238123516 2 52 -7.736657834584074 2 53 -6.780371991797111 2 54 -15.658208431015236 2 55 -8.762901981374817 2 56 -7.852735744708612 3 1 0.9098055415826991 3 2 1.1722890611008907 3 3 1.7710425371375464 3 4 1.588926136868693 3 5 3.6159721561216145 3 6 1.3560246859277214 3 7 2.015678413246121 3 8 2.257544156246105 3 9 1.9927316719767063 3 10 1.6542074778789981 3 11 1.634252330640507 3 12 2.2233933858235773 3 13 -3.3760354745932943 3 14 -4.5817979208996 3 15 -3.571355882264715 3 16 2.5121492500742946 3 17 2.7378396409545402 3 18 2.600589895021848 3 19 0.9322974395753576 3 20 1.070048454352353 3 21 2.814577054359532 3 22 2.9532015374825136 3 23 2.0184981573190726 3 24 3.7370006255868304 3 25 2.744567641594621 3 26 2.7766634185740955 3 27 2.238169360463381 3 28 1.8584294495333396 3 29 1.6647075931560948 3 30 1.552180840196229 3 31 1.2593196234543953 3 32 0.7044035513162754 3 33 1.2114258789287575 3 34 2.30929531160902 3 35 1.6851957477363797 3 36 3.4621358528841775 3 37 3.5751935894859947 3 38 3.9015059915438037 3 39 7.080634447671983 3 40 9.691147838212771 3 41 7.807837227802519 3 42 5.987152796691493 3 43 0.5195654889182606 3 44 2.0172840831761896 3 45 6.623715537250291 3 46 0.060314304682805187 3 47 -3.738710027685901 3 48 -0.6573671381639095 3 49 -8.593099248314337 3 50 -2.05702477157174 3 51 -2.2294672976258596 3 52 -1.7860628111437578 3 53 -2.3231054934199022 3 54 -6.304439949226559 3 55 -3.2609185699070906 3 56 -2.14123195819581 4 1 1.2233648636165952 4 2 1.799972676920099 4 3 1.6690651360076032 4 4 1.6527679447782972 4 5 3.765689670245905 4 6 1.9698664938373227 4 7 2.27923773528002 4 8 2.3188435912743515 4 9 1.9074209375134288 4 10 1.6081622801388846 4 11 2.977472669623559 4 12 3.162658922546747 4 13 5.139783734446258 4 14 2.8854337175184774 4 15 6.061129993441497 4 16 6.231358289622321 4 17 5.1680656296551035 4 18 5.394657691632013 4 19 3.7362522418352455 4 20 3.9152461944653467 4 21 3.2419781843030293 4 22 2.984274983810195 4 23 3.028667648844497 4 24 3.6949102301066077 4 25 3.5945676415946224 4 26 2.9232735880656193 4 27 2.092406648598974 4 28 2.1355480936011375 4 29 2.2977584406137197 4 30 1.5414463769193958 4 31 2.6782461771267094 4 32 2.355815980694807 4 33 2.1444767263863866 4 34 2.163815085620321 4 35 2.931523431352204 4 36 4.7638307681384156 4 37 4.428865905870172 4 38 4.438229155385617 4 39 7.6995610013443 4 40 9.704989646122371 4 41 2.8358033294974376 4 42 7.708339237369458 4 43 7.1848197262063955 4 44 10.476041145323084 4 45 7.5742805090017065 4 46 8.106077016547214 4 47 7.9533803677943276 4 48 7.449695008728746 4 49 5.97639227710939 4 50 8.291845284925436 4 51 7.650193719323294 4 52 8.237101030664153 4 53 6.617854958557501 4 54 1.8478199377790894 4 55 6.821849791674829 4 56 7.7121578723126625 5 1 1.5597284999802312 5 2 1.8696696466170692 5 3 2.4887621057045735 5 4 2.092161884172235 5 5 3.744477549033789 5 6 2.059260433231266 5 7 2.02923773528002 5 8 2.6627829852137452 5 9 2.6559057859982786 5 10 2.0369501589267642 5 11 2.718381760532651 5 12 5.324780134667954 5 13 6.227662522325044 5 14 5.906645838730601 5 15 6.341433023744528 5 16 6.464691622955648 5 17 6.151398962988438 5 18 5.571930418904742 5 19 5.387767393350394 5 20 4.6728219520411045 5 21 3.931372123696974 5 22 3.9266992262344402 5 23 3.9195767397535874 5 24 3.938849624046 5 25 2.908204005230985 5 26 2.373273588065615 5 27 2.6560430122353367 5 28 2.9219117299647763 5 29 2.9386675315228103 5 30 2.908113043586063 5 31 3.7767310256115607 5 32 4.42551295039177 5 33 3.7035676354772917 5 34 3.344118115923351 5 35 3.555765855594629 5 36 5.345648949956594 5 37 5.57735075435502 5 38 6.523077640234103 5 39 8.488954940738239 5 40 6.92165631278904 5 41 1.4130760567701621 5 42 9.531066510096732 5 43 12.59845608984276 5 44 13.160889630171567 5 45 10.766704751425948 5 46 12.35153156200176 5 47 12.132168246582204 5 48 12.475452584486323 5 49 11.576392277109392 5 50 11.802451345531498 5 51 11.132011901141471 5 52 10.385585879149001 5 53 10.44361253431508 5 54 10.55842599838515 5 55 10.432455852280889 5 56 9.443976054130847 6 1 1.77791031816205 6 2 2.233306010253429 6 3 1.8705802875227562 6 4 2.4921618841722335 6 5 3.762659367215605 6 6 2.58653316050399 6 7 2.847419553461833 6 8 2.9355102579410186 6 9 2.892269422361913 6 10 2.6369501589267585 6 11 3.8092908514417374 6 12 6.215689225577044 6 13 6.918571613234132 6 14 7.233918566003329 6 15 7.086887569199078 6 16 7.264691622955652 6 17 7.0423080538975285 6 18 6.8264758734501925 6 19 4.933221938804941 6 20 5.127367406586558 6 21 3.440463032787882 6 22 4.326699226234439 6 23 3.4468494670263183 6 24 4.175213260409635 6 25 3.2718403688673448 6 26 3.5005463153383474 6 27 3.1833157395080605 6 28 2.8673662754193217 6 29 3.665940258795537 6 30 3.253567589040607 6 31 4.231276480157014 6 32 5.8618765867554075 6 33 3.4490221809318413 6 34 4.780481752286988 6 35 3.392129491958265 6 36 4.582012586320229 6 37 4.650078027082291 6 38 5.813986731143192 6 39 6.37986403164733 6 40 1.6307472218799504 6 41 3.6676215113156196 6 42 7.440157419187642 6 43 11.816637908024578 6 44 11.379071448353386 6 45 7.639432024153219 6 46 10.569713380183579 6 47 11.223077337491297 6 48 10.91181622084996 6 49 8.81275591347303 6 50 9.820633163713314 6 51 10.295648264777835 6 52 10.549222242785365 6 53 10.370885261587802 6 54 11.031153271112426 6 55 9.177910397735435 6 56 9.571248781403572 7 1 1.704496939088294 7 2 1.6446267649704112 7 3 1.7467380919824436 7 4 1.8156610265393027 7 5 3.9009097960320744 7 6 1.7337029718247479 7 7 2.489615093770581 7 8 1.803434786242903 7 9 2.440125340029155 7 10 1.6084767455476907 7 11 3.491623613019783 7 12 4.7680048344964305 7 13 6.0259472564588314 7 14 6.225685289845526 7 15 6.170249490296847 7 16 7.117521811634894 7 17 6.2306442460073015 7 18 5.282393540688616 7 19 4.622415763847819 7 20 3.8196487101886163 7 21 2.458644850969698 7 22 3.6855328454454153 7 23 2.685271422429402 7 24 3.192708972244972 7 25 2.669095943481416 7 26 1.8751603805184516 7 27 1.0773123089763317 7 28 2.0515858294501967 7 29 2.1571924028778753 7 30 1.8703771945294605 7 31 2.394912843793378 7 32 4.072168181952662 7 33 1.4963635188392175 7 34 2.6936892994568 7 35 1.8044794061949716 7 36 0.4883590700252043 7 37 1.3049665348009931 7 38 2.5577260107315247 7 39 4.2916993661241705 7 40 -3.0821172721166192 7 41 -8.929291010125205 7 42 4.654565652463798 7 43 9.491423499791303 7 44 8.825726679914279 7 45 4.751953464976548 7 46 8.93815248824533 7 47 9.107468418108791 7 48 8.804097524452015 7 49 11.104694163901843 7 50 8.667631448447448 7 51 10.738872964606312 7 52 9.867918640727044 7 53 10.757163134658128 7 54 11.434926856018084 7 55 10.554239728781745 7 56 9.647063532690023 8 1 1.2633648636165944 8 2 1.7533060102534321 8 3 1.6123984693409383 8 4 1.6394346114449618 8 5 3.7990230035792436 8 6 1.8265331605039918 8 7 1.8492377352800204 8 8 2.4155102579410155 8 9 2.0740876041801 8 10 1.4514956134722183 8 11 2.227472669623559 8 12 4.97932558921341 8 13 5.63311706777959 8 14 5.932100384185144 8 15 5.097796660108166 8 16 6.52469162295565 8 17 6.3513989629884335 8 18 5.664657691632016 8 19 4.4095855751685775 8 20 4.341912861132016 8 21 3.0586448509696993 8 22 3.2976083171435278 8 23 2.588667648844499 8 24 3.131576896773275 8 25 2.984567641594623 8 26 2.893273588065618 8 27 3.132406648598973 8 28 2.465548093601136 8 29 3.0477584406137197 8 30 2.2681130435860624 8 31 2.614912843793377 8 32 4.089149314028134 8 33 2.3544767263863804 8 34 1.740481752286982 8 35 2.104856764685536 8 36 0.6638307681384141 8 37 1.1355325725368424 8 38 1.7048958220522827 8 39 2.3762276680109657 8 40 -4.158343687210959 8 41 -10.534196670502563 8 42 3.778339237369458 8 43 8.474819726206395 8 44 7.54270781198975 8 45 4.677613842335038 8 46 7.386077016547212 8 47 7.76671370112766 8 48 7.646361675395411 8 49 9.576392277109392 8 50 7.435178618258767 8 51 8.930193719323295 8 52 9.723767697330818 8 53 9.114521625224167 8 54 8.851153271112423 8 55 8.185183125008162 8 56 6.3221578723126655 9 1 1.3829393317016994 9 2 1.5992634570619444 9 3 2.397079320404764 9 4 2.079009079530067 9 5 3.9820017269834977 9 6 1.484405500929526 9 7 2.361152628897038 9 8 2.722744300494213 9 9 2.6825982424779724 9 10 2.4446871028339174 9 11 3.695557776006538 9 12 5.196346865809154 9 13 6.3603511103327826 9 14 6.962738682057488 9 15 7.165030702661362 9 16 6.154053325083311 9 17 5.955654282137374 9 18 5.755295989504354 9 19 4.7440536602749575 9 20 4.249997967514993 9 21 3.03311293607608 9 22 3.668672146930767 9 23 3.2244123296955607 9 24 3.5307258329434887 9 25 2.595631471381857 9 26 2.275401247640083 9 27 2.8328321805138685 9 28 2.6983140510479444 9 29 2.264779717209464 9 30 2.3123683627350005 9 31 3.2885298650699752 9 32 3.980638675730262 9 33 2.088093747662981 9 34 2.835800901223159 9 35 3.1882610200046813 9 36 1.1808520447341557 9 37 1.6678729980687557 9 38 2.4240447582224967 9 39 3.5992063914152226 9 40 -2.2868543255088305 9 41 -5.8758987981621384 9 42 3.8753605139652016 9 43 10.158223981525545 9 44 9.202282280074858 9 45 5.763996821058441 9 46 9.720970633568491 9 47 10.425011573468083 9 48 9.65997869667201 9 49 12.406179511151944 9 50 9.832625426769408 9 51 9.46508733634457 9 52 7.667597484564862 9 53 8.772819497564594 9 54 9.554557526431573 9 55 7.194544827135822 9 56 7.551519574440324 10 1 2.0711909505731185 10 2 2.094175575470821 10 3 2.8697897736887654 10 4 2.5063911331840956 10 5 4.577283873144459 10 6 2.5995766387648587 10 7 3.0466290396278453 10 8 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1.2286676488444996 115 24 0.41157689677327625 115 25 1.0445676415946181 115 26 1.3066069213989522 115 27 0.9257399819323027 115 28 -0.48111857306552963 115 29 1.1477584406137211 115 30 1.0081130435860644 115 31 -0.005087156206620591 115 32 -4.57751735263853 115 33 0.7944767263863852 115 34 0.18048175228698682 115 35 0.6315234313521998 115 36 3.097164101471744 115 37 2.995532572536842 115 38 4.038229155385611 115 39 6.38289433467763 115 40 10.088322979455711 115 41 7.085803329497438 115 42 2.3916725707027915 115 43 -0.26518027379360376 115 44 -1.323958854676917 115 45 2.724280509001705 115 46 -3.293922983452788 115 47 -5.446619632205675 115 48 -5.000304991271253 115 49 -10.190274389557276 115 50 -0.6248213817412314 115 51 2.0835270526566276 115 52 3.0371010306641537 115 53 1.2678549585574999 115 54 2.0978199377790894 115 55 2.2718497916748284 115 56 3.4954912056459975 116 1 1.0447934350451646 116 2 1.1547345816819998 116 3 1.830969897912368 116 4 1.5980060400163936 116 5 2.456165860722095 116 6 1.1508188747897066 116 7 1.8292377352800173 116 8 2.114081686512449 116 9 2.0169447470372432 116 10 1.1772098991865008 116 11 -1.4868130446621564 116 12 -3.9921029822151617 116 13 -4.712597217934697 116 14 -8.72647104438628 116 15 -7.72934619703469 116 16 -13.406736948472922 116 17 -9.87717246558299 116 18 -5.112485165510844 116 19 -5.223271567688567 116 20 -6.146658567439413 116 21 -0.6127837204588715 116 22 -0.18239168285646912 116 23 -0.4427609225840712 116 24 0.390148325344704 116 25 0.5302819273089057 116 26 -0.1410121262200974 116 27 0.02812093431325735 116 28 0.27126237931542363 116 29 1.6120441548994364 116 30 0.5866844721574935 116 31 -2.9622300133494797 116 32 -8.996564971686148 116 33 -2.2698089878993315 116 34 -1.6695182477130146 116 35 -1.6065718067430375 116 36 1.5638307681384127 116 37 2.152675429679693 116 38 3.3548958220522813 116 39 4.8590848108681115 116 40 8.235942027074756 116 41 5.985803329497436 116 42 2.1154820945123163 116 43 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1.1724066485989724 119 28 0.6855480936011418 119 29 1.2077584406137234 119 30 1.2681130435860624 119 31 -0.16508715620662429 119 32 -5.730850685971863 119 33 0.1944767263863838 119 34 -1.3795182477130155 119 35 -0.05514323531446763 119 36 1.2838307681384116 119 37 1.5955325725368361 119 38 2.4248958220522816 119 39 5.096227668010968 119 40 6.601656312789039 119 41 6.005803329497436 119 42 0.4583392373694579 119 43 -0.6051802737936036 119 44 -0.7772921880102501 119 45 -2.822386157664962 119 46 -6.393922983452786 119 47 -8.37328629887234 119 48 -10.033638324604588 119 49 -7.5836077228906085 119 50 -4.52482138174123 119 51 -4.969806280676707 119 52 -3.9962323026691813 119 53 -6.985478374775832 119 54 -8.048846728887577 119 55 -7.454816874991838 119 56 -2.5378421276873375 120 1 -0.5566351363834059 120 2 -0.20669398974656872 120 3 -0.6676015306590628 120 4 -0.34056538855503504 120 5 2.0390230035792385 120 6 -1.0534668394960107 120 7 -0.9707622647199798 120 8 0.45551025794102173 120 9 0.8340876041800982 120 10 -1.4685043865277834 120 11 -8.17252733037644 120 12 -3.02067441078659 120 13 -7.866882932220413 120 14 -6.947899615814851 120 15 -8.422203339891833 120 16 -9.77530837704435 120 17 -10.488601037011563 120 18 -3.035342308367987 120 19 -3.1504144248314248 120 20 -5.058087138867986 120 21 -1.5813551490303013 120 22 -1.422391682856471 120 23 -3.1713323511555025 120 24 -7.028423103226725 120 25 -2.1354323584053816 120 26 -0.5867264119343787 120 27 -0.4675933514010282 120 28 -0.8744519063988605 120 29 -1.1922415593862823 120 30 -0.7718869564139368 120 31 -3.4050871562066227 120 32 -9.490850685971864 120 33 -2.645523273613616 120 34 -11.819518247713013 120 35 -3.855143235314465 120 36 -3.116169231861587 120 37 -3.7244674274631606 120 38 -2.055104177947719 120 39 1.0562276680109655 120 40 -2.198343687210958 120 41 2.205803329497435 120 42 -4.421660762630541 120 43 0.43481972620639553 120 44 -3.2972921880102497 120 45 -6.3823861576649605 120 46 -13.273922983452787 120 47 -12.81328629887234 120 48 -11.153638324604588 120 49 -13.623607722890608 120 50 -10.924821381741232 120 51 -10.329806280676708 120 52 -10.03623230266918 120 53 -10.385478374775833 120 54 -9.408846728887577 120 55 -10.574816874991837 120 56 -5.937842127687336 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 171.0 5 44.0 6 53.0 7 45.0 8 80.0 9 72.0 10 61.0 11 66.0 12 49.0 13 60.0 14 76.0 15 105.0 16 140.0 17 219.0 18 275.0 19 347.0 20 444.0 21 510.0 22 678.0 23 822.0 24 960.0 25 1049.0 26 1157.0 27 1163.0 28 1292.0 29 1339.0 30 1427.0 31 1612.0 32 2088.0 33 2465.0 34 2941.0 35 3480.0 36 4088.0 37 3801.0 38 1931.0 39 482.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 100.0 0.0 3 100.0 0.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 99.173971679029 0.6771184535850753 0.13486176668914363 0.014048100696785797 8 99.33692964711172 0.4720161834120027 0.11800404585300067 0.07305012362328614 9 98.20971867007673 1.1298164750850173 0.3063432730951912 0.35412158174306513 10 46.58912115082041 27.843335581029443 17.3072600584401 8.260283209710046 11 30.710010957826416 35.612935854569976 16.054620550139077 17.622432637464527 12 30.52652281411553 13.559226792537649 18.790739492020677 37.12351090132614 13 31.436839739267253 12.061699258260283 22.271858844684196 34.22960215778827 14 20.520028109627546 15.0049191848208 25.9451862262825 38.529866479269145 15 25.38645642686189 21.238913135294947 23.575953822328593 29.79867661551457 16 29.221126008376196 16.02439778508587 21.58416954774152 33.17030665879641 17 26.17247871413719 27.667406637255176 21.586534408632367 24.57358023997527 18 34.369204832818205 22.188817083450406 21.983703287440292 21.458274796291093 19 27.78933168455961 22.455173964364004 26.05811927379012 23.697375077286267 20 24.958538299367532 24.317638791286015 28.416022487702037 22.307800421644412 21 25.93289872990896 19.582443520287736 23.20164100258514 31.283016747218163 22 25.47482580355136 19.479096426163185 29.869071701505955 25.1770060687795 23 34.40941784670712 21.15363002922005 21.38401888064734 23.05293324342549 24 25.044953922229713 29.194762868060238 22.68768262530906 23.07260058440099 25 34.74095302315127 21.237918633400767 20.577657900651833 23.443470442796134 26 22.21596667042 21.21664227001464 30.99313140412116 25.57425965544421 27 21.727277837108648 18.492496205946825 23.601821145523015 36.17840481142151 28 29.529521074415072 23.03967114339612 24.182785708252386 23.248022073936426 29 32.37220020796448 20.6727931877582 25.233960037096363 21.721046567180956 30 21.900559318699234 27.991230782202987 29.6776188200905 20.43059107900728 31 30.543037404236138 22.053853086976115 27.380013519603423 20.023095989184316 32 26.802675209351996 24.14713651435958 27.005001967065702 22.04518630922273 33 23.668355855855854 18.313626126126124 29.25957207207207 28.758445945945947 34 21.005850585058504 29.5004500450045 31.193744374437443 18.29995499549955 35 21.229757085020243 21.85672514619883 33.58074673864147 23.33277103013945 36 28.289140347914206 24.09784383268592 29.111073579913306 18.501942239486574 37 21.276236089590085 21.239611212846878 31.965065502183403 25.51908719537963 38 25.68324467336542 29.226828787750854 29.218384981282895 15.871541557600832 39 27.0464742233133 18.80414232907249 25.494511696153953 28.654871751460252 40 18.740803621958122 14.708545557441994 23.455008488964346 43.095642331635545 41 20.84149005589128 16.222657247425314 25.56245921638721 37.373393480296194 42 20.41684269821152 28.58188987466554 31.94479650753415 19.05647091958879 43 12.777777777777777 12.287084038353074 30.620417371686408 44.31472081218274 44 18.125547610299314 13.83510923942229 22.94451824425539 45.09482490602301 45 20.03151024082827 27.025658338960163 33.448683322079674 19.49414809813189 46 25.484406198149557 20.900475266458564 31.78942039989876 21.825698135493123 47 16.952439847087923 26.666854058916122 31.06026534742523 25.32044074657072 48 20.335647822786946 23.07086835521322 38.66415539875748 17.929328423242346 49 17.63312274368231 15.379625451263538 23.716719314079423 43.27053249097473 50 27.95323284969714 21.236793914635864 32.134103394844345 18.67586984082265 51 22.546613635722544 24.38859270542439 31.736763419931734 21.32803023892133 52 21.111581323176754 22.431936803498377 33.71702637889688 22.739455494427986 53 19.531425996846135 28.81279567470151 33.17751745888714 18.478260869565215 54 17.491136248522707 27.010523946198433 28.470932522933197 27.027407282345656 55 19.564850258950685 29.841251970276968 31.873451925242062 18.720445845530286 56 21.776100912264894 28.891203964410405 30.24270751210722 19.08998761121748 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 1.0 9 1.0 10 0.5 11 0.0 12 8.5 13 41.0 14 65.0 15 71.0 16 77.0 17 97.0 18 117.0 19 145.5 20 174.0 21 190.5 22 231.0 23 255.0 24 339.0 25 423.0 26 531.5 27 640.0 28 715.0 29 850.0 30 910.0 31 955.0 32 1000.0 33 1000.5 34 1001.0 35 910.0 36 819.0 37 755.0 38 608.5 39 526.0 40 514.0 41 502.0 42 513.5 43 525.0 44 584.0 45 643.0 46 730.0 47 966.5 48 1116.0 49 1142.0 50 1168.0 51 1204.0 52 1240.0 53 1462.0 54 2020.0 55 2356.0 56 2291.0 57 2226.0 58 2208.5 59 2191.0 60 2268.0 61 2345.0 62 2341.5 63 2219.0 64 2100.0 65 1949.5 66 1799.0 67 1585.5 68 1372.0 69 1276.5 70 1181.0 71 1023.0 72 737.5 73 610.0 74 499.0 75 388.0 76 300.0 77 212.0 78 152.5 79 76.0 80 59.0 81 46.0 82 33.0 83 23.0 84 13.0 85 7.0 86 1.0 87 0.5 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.030905821532928746 10 0.0 11 0.0028096201393571587 12 0.0 13 0.0 14 0.0477635423690717 15 0.2163407507305012 16 0.04214430209035738 17 0.014048100696785797 18 0.005619240278714317 19 0.028096201393571593 20 0.0477635423690717 21 0.011238480557428635 22 0.0 23 0.0 24 0.0 25 0.0 26 0.1910541694762868 27 0.028096201393571593 28 0.21072151045178691 29 0.02528658125421443 30 0.03652506181164307 31 0.24724657226342997 32 0.016857720836142953 33 0.20229265003371544 34 0.11238480557428637 35 0.06743088334457181 36 0.18543492919757248 37 0.2725331535176444 38 0.177006068779501 39 0.42987188132164533 40 0.708024275118004 41 0.9693189480782198 42 0.24443695212407282 43 0.37086985839514497 44 0.5928298494043605 45 0.13486176668914363 46 0.09271746459878624 47 0.04495392222971454 48 0.053382782647786016 49 0.3821083389525736 50 0.2725331535176444 51 0.39615643964935937 52 0.4130141604855023 53 0.22476961114857275 54 0.15171948752528658 55 0.17981568891885816 56 0.21353113059114406 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 35592.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.96055293324343 #Duplication Level Percentage of deduplicated Percentage of total 1 93.61894607605569 81.41155315801304 2 4.2001873929759945 7.305012362328613 3 1.053277761623211 2.7478084962913014 4 0.361862298471778 1.2587098224320072 5 0.25524215695777197 1.1097999550460778 6 0.14216018868534133 0.7417397167902899 7 0.07108009434267067 0.4326815014610024 8 0.05169461406739686 0.3596313778377163 9 0.02584730703369843 0.20229265003371544 >10 0.1970857161319505 3.0849629130141607 >50 0.022616393654486124 1.3458080467520792 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTGGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 95 0.2669139132389301 No Hit TTGGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 86 0.24162733198471567 No Hit TTGGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 71 0.1994830298943583 No Hit TTGGACGGGTACGCACAGTTCTGAGTCGGAGACACGCAGGCATGAGATGGGCAAAA 65 0.18262530905821533 No Hit TTGGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 55 0.15452910766464373 No Hit TTGGACGGGTACGCACAGTTCTGAGTCGGAGACACGCAGCCATGAGATGGCCAAAA 54 0.15171948752528658 No Hit TTGGACGGGTACGCACAGTTCTGAGTCGGAGACACGCAGGCATGAGATGGCCAAAA 53 0.14890986738592943 No Hit TTGGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG 49 0.13767138682850077 No Hit TTGGACGGGTACGCACAGTTCTGAGTCGGAGACACGCAGCCATGAGATGGGCAAAA 45 0.12643290627107215 No Hit TTGGACGGGTACGCACAGTTCTGAGTCGGAGACACGCAGGCATGAGATGGGGAAAA 43 0.12081366599235784 No Hit TTGGACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC 42 0.11800404585300067 No Hit TTGGACGGGTACGCACAGTTCTGAGTCGGAGACACGCAGCGATGAGATGGCCAAAA 39 0.1095751854349292 No Hit TTGGACGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC 36 0.10114632501685772 No Hit TTGGACGGGTACGCACAGTTCTGAGTCGGAGACACGCAGGGATCAGATCGCCAAAA 36 0.10114632501685772 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0028096201393571587 0.0 0.0 0.0 0.0 8 0.0028096201393571587 0.0 0.0 0.0 0.0 9 0.0028096201393571587 0.0 0.0 0.0 0.0 10 0.0028096201393571587 0.0 0.0 0.0 0.0 11 0.014048100696785795 0.0 0.0 0.0 0.0 12 0.014048100696785795 0.0 0.0 0.0 0.0 13 0.014048100696785795 0.0 0.0 0.0 0.0 14 0.019667340975500113 0.0 0.0 0.0 0.0 15 0.02247696111485727 0.0 0.0 0.0 0.0 16 0.02528658125421443 0.0 0.0 0.0 0.0 17 0.04495392222971454 0.0 0.0 0.0 0.0 18 0.0477635423690717 0.0 0.0 0.0 0.0 19 0.059002022926500336 0.0 0.0 0.0 0.0 20 0.059002022926500336 0.0 0.0 0.0 0.0 21 0.06743088334457181 0.0 0.0 0.0 0.0 22 0.07866936390200045 0.0 0.0 0.0 0.0 23 0.08428860418071477 0.0 0.0 0.0 0.0 24 0.08990784445942908 0.0 0.0 0.0 0.0 25 0.09271746459878624 0.0 0.0 0.0 0.0 26 0.10114632501685772 0.0 0.0 0.0 0.0 27 0.10395594515621488 0.0 0.0 0.0 0.0 28 0.1095751854349292 0.0 0.0 0.0 0.0 29 0.1095751854349292 0.0 0.0 0.0 0.0 30 0.11519442571364351 0.0 0.0 0.0 0.0 31 0.11519442571364351 0.0 0.0 0.0 0.0 32 0.11519442571364351 0.0 0.0 0.0 0.0 33 0.11519442571364351 0.0 0.0 0.0 0.0 34 0.11519442571364351 0.0 0.0 0.0 0.0 35 0.11519442571364351 0.0 0.0 0.0 0.0 36 0.11519442571364351 0.0 0.0 0.0 0.0 37 0.11519442571364351 0.0 0.0 0.0 0.0 38 0.11519442571364351 0.0 0.0 0.0 0.0 39 0.11519442571364351 0.0 0.0 0.0 0.0 40 0.11519442571364351 0.0 0.0 0.0 0.0 41 0.11800404585300067 0.0 0.0 0.0 0.0 42 0.12081366599235784 0.0 0.0 0.0 0.0 43 0.123623286131715 0.0 0.0 0.0 0.0 44 0.123623286131715 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCATGA 25 1.920792E-5 50.536377 39 CCGTCTC 20 3.9164588E-4 50.536377 40 CCCGTCT 20 3.9164588E-4 50.536377 39 GCTTGTC 25 1.920792E-5 50.536377 38 TTGTCTC 30 9.4653296E-7 50.536373 40 TTCGTTT 15 0.008018004 50.536373 38 GGATCAG 30 9.4653296E-7 50.536373 40 TCGTTTT 15 0.008018004 50.536373 39 GACCAGC 15 0.008018004 50.536373 41 CGTCTCA 30 9.4653296E-7 50.536373 41 TATGCTT 45 1.200533E-10 50.321026 35 TGCTTGT 25 1.9701109E-5 50.321026 37 ATGCTTG 45 1.200533E-10 50.321026 36 GCCCCAC 15 0.008154787 50.321022 37 TTTTCGT 15 0.008154787 50.321022 36 TGCTTGC 20 4.0001006E-4 50.321022 37 CAGCTCG 20 4.0282944E-4 50.249645 44 CTCCACG 20 4.0282944E-4 50.249645 44 ACCAACA 15 0.008200766 50.249645 42 CAACACA 20 4.0282944E-4 50.249645 44 >>END_MODULE