FastQCFastQC Report
Fri 17 Jun 2016
SRR1526322_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526322_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214747
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG20680.9629936623096016No Hit
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG15460.7199169254983772No Hit
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG14540.6770758147960158No Hit
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG10600.49360410157068557No Hit
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG8490.3953489455033132No Hit
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAACGATTAAG6060.2821925335394674No Hit
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC4900.22817548091475084No Hit
TCACTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC4780.2225875099535733No Hit
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3910.1820747204850359No Hit
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTCAG3760.17508975678356392No Hit
TCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC3750.1746240925367991No Hit
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGCCTCAAAGATTAAG3350.1559975226662072No Hit
TCACTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC3330.1550661941726776No Hit
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG2970.1383022812891449No Hit
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC2730.12712633936678977No Hit
TCACTTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGCGCTAATACATGCCGAC2720.12666067512002496No Hit
TCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGACCACCCGACTGCTCTTC2690.12526368237973057No Hit
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG2670.12433235388620097No Hit
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTCAG2480.11548473319766982No Hit
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAAAGATTAAG2250.10477445552207947No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG150.00797462450.6945238
ATGCGTC150.00797462450.6945238
TTCGACC150.00797462450.6945238
TACCGCT150.00797462450.6945238
CGCGAAT150.00804219950.58645241
AAGACTA204.007553E-450.41926236
CTGCAAT150.00827052550.2295449
CGATTGT252.0345211E-550.19412644
CGTGCAA355.113361E-850.18233548
ACACGAT150.0083013350.1823346
ACCGAAT150.0083013350.1823342
ATATGCG150.0083013350.1823334
TATTGTC150.0083013350.1823342
TACATCC150.0083013350.1823334
CTACGAA150.0083013350.1823346
ACGATTA5750.050.18232748
CACGATT2250.050.1705447
TCGCATA252.103383E-549.91259830
CGAGTAA252.103383E-549.91259831
TAGCATT150.00848011249.91259830
Produced by FastQC (version 0.11.5)