##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526322_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 214747 Sequences flagged as poor quality 0 Sequence length 56 %GC 53 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.88984712242779 39.0 38.0 40.0 35.0 40.0 2 37.22308577069761 39.0 38.0 40.0 33.0 40.0 3 36.12451396294244 39.0 38.0 40.0 28.0 40.0 4 37.264013001345766 39.0 38.0 40.0 33.0 40.0 5 37.5942853683637 39.0 38.0 40.0 35.0 40.0 6 37.77327739153515 39.0 38.0 40.0 35.0 40.0 7 37.64792523294854 39.0 38.0 40.0 34.0 40.0 8 37.35211667683367 39.0 38.0 40.0 33.0 40.0 9 37.34176961727056 39.0 38.0 40.0 33.0 40.0 10 37.09308628292828 39.0 38.0 40.0 33.0 40.0 11 35.90463661890504 38.0 35.0 39.0 30.0 40.0 12 34.009392447857245 38.0 32.0 39.0 22.0 40.0 13 33.51193730296581 38.0 32.0 39.0 19.0 40.0 14 32.71125557050855 38.0 30.0 39.0 16.0 40.0 15 33.30658402678501 38.0 31.0 39.0 19.0 40.0 16 32.926751013983896 37.0 30.0 39.0 18.0 40.0 17 32.96003203770018 37.0 30.0 39.0 19.0 40.0 18 33.33016060759871 37.0 31.0 39.0 22.0 40.0 19 34.03407730957825 37.0 32.0 39.0 25.0 40.0 20 34.19903421235221 37.0 32.0 39.0 25.0 40.0 21 35.52567439824538 38.0 35.0 39.0 28.0 40.0 22 35.256720699241434 38.0 35.0 39.0 27.0 40.0 23 35.522507881367375 38.0 35.0 39.0 28.0 40.0 24 35.09237381663073 38.0 35.0 39.0 26.0 40.0 25 35.37226596879118 38.0 35.0 39.0 28.0 40.0 26 35.679655594723094 38.0 35.0 39.0 29.0 40.0 27 35.65983226773832 38.0 35.0 39.0 29.0 40.0 28 35.316768103861754 38.0 35.0 39.0 28.0 40.0 29 35.287659431796484 38.0 35.0 39.0 28.0 40.0 30 35.05145124262504 38.0 35.0 39.0 27.0 40.0 31 34.649494521460134 38.0 35.0 39.0 26.0 40.0 32 33.65131526866499 38.0 33.0 39.0 19.0 40.0 33 35.05175392438544 38.0 35.0 39.0 27.0 40.0 34 34.76420625200818 38.0 35.0 39.0 27.0 40.0 35 34.844514708005235 38.0 35.0 39.0 27.0 40.0 36 33.01807242941694 36.0 32.0 38.0 24.0 39.0 37 32.8530689602183 36.0 32.0 38.0 23.0 39.0 38 32.07698826991762 36.0 31.0 38.0 18.0 39.0 39 30.175834819578387 35.0 28.0 38.0 8.0 39.0 40 25.997038375390577 31.0 13.0 38.0 4.0 39.0 41 23.01504561181297 27.0 10.0 34.0 4.0 38.0 42 26.67441687194699 31.0 23.0 35.0 4.0 38.0 43 22.306411731013704 26.0 9.0 33.0 4.0 37.0 44 23.797929656758885 29.0 11.0 34.0 4.0 37.0 45 26.228263957121637 31.0 22.0 35.0 4.0 38.0 46 25.94514009508864 31.0 19.0 36.0 4.0 38.0 47 25.25025262285387 30.0 15.0 36.0 4.0 38.0 48 25.2598779028345 30.0 16.0 36.0 4.0 38.0 49 22.70718100834936 27.0 7.0 35.0 4.0 38.0 50 25.149506163066306 30.0 16.0 36.0 4.0 38.0 51 26.61264185297117 33.0 15.0 38.0 4.0 40.0 52 26.543774767517125 33.0 11.0 38.0 4.0 40.0 53 26.141845986207024 32.0 7.0 38.0 4.0 40.0 54 25.155764690542824 32.0 4.0 38.0 4.0 40.0 55 25.4058729574802 32.0 4.0 38.0 4.0 39.0 56 24.8405612185502 32.0 4.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.6200713856110553 1 2 1.6930347399024868 1 3 2.8193019506795025 1 4 1.3575400459646474 1 5 0.930369406236558 1 6 0.8597754468834182 1 7 1.0334274589697756 1 8 1.2603104259981848 1 9 1.2992959235770627 1 10 1.2613727482946047 1 11 -3.009036998264513 1 12 -7.3278410597286445 1 13 -21.378765875603147 1 14 -17.58304860323692 1 15 -9.75495440568459 1 16 -20.834009494950486 1 17 -21.66281149519155 1 18 -20.460146151692825 1 19 -6.032849045508659 1 20 -10.071304543776012 1 21 -6.49615493765593 1 22 -2.3508261444786207 1 23 -2.663781342018858 1 24 -2.8352418320964254 1 25 -2.99542529096313 1 26 2.1726631347253544 1 27 1.9408915955526638 1 28 2.0574352827083615 1 29 1.6634957482854134 1 30 1.8095762781940792 1 31 1.4564404582130805 1 32 -11.497152753192545 1 33 0.7437528927260004 1 34 0.7459522623500447 1 35 0.8084529358072459 1 36 3.21537079914782 1 37 3.444417100523058 1 38 3.980269134045912 1 39 6.031460369538777 1 40 8.582034635447368 1 41 -0.9369082029888993 1 42 -6.215515573357727 1 43 -12.972179258990124 1 44 -16.470796355556754 1 45 -13.223220363172297 1 46 -18.519959850299987 1 47 -18.563400619267103 1 48 -16.22624992735299 1 49 -14.803694696979129 1 50 -16.559335171243028 1 51 -17.80375770214255 1 52 -17.63456559015912 1 53 -17.578356382200298 1 54 -18.807763888869694 1 55 -17.045174823053216 1 56 -16.13499787278545 2 1 0.5248986562585074 2 2 1.6055833557798564 2 3 2.880901127069343 2 4 1.2253048595084621 2 5 1.026743233735985 2 6 0.8180801826418218 2 7 1.0012551871784225 2 8 1.2486998105783726 2 9 1.3561765001041692 2 10 1.042744287988036 2 11 -2.344372161842635 2 12 -11.046240739435806 2 13 -12.651958508867846 2 14 -15.533317420441222 2 15 -8.180514918153133 2 16 -2.69019229064941 2 17 -2.333996578696471 2 18 -10.297483145515752 2 19 -2.200430834572945 2 20 -3.1709956899668157 2 21 2.1941047283243975 2 22 2.5180253769123695 2 23 1.915205159925776 2 24 2.350442221895797 2 25 2.1046948188515557 2 26 2.188105825185204 2 27 2.169414816783501 2 28 2.168079300095691 2 29 2.164210687658553 2 30 2.6852626199922938 2 31 -0.9943145177649555 2 32 -9.672141314162573 2 33 -0.6883278668255883 2 34 -0.40026713824478577 2 35 -0.6936632847372479 2 36 3.741823555954042 2 37 4.204426251747034 2 38 4.812029600758336 2 39 6.775168903055139 2 40 9.813617797195253 2 41 7.267936226203506 2 42 3.541090466450097 2 43 -1.4586526060502933 2 44 -9.754627286693793 2 45 1.4014078683536688 2 46 -11.314429079309368 2 47 -12.264870534618282 2 48 -8.086693761715832 2 49 -11.434013845915299 2 50 -8.208957683254011 2 51 -7.363240657987895 2 52 -7.261624615553764 2 53 -8.021418610523337 2 54 -15.221084639270059 2 55 -8.481659609143357 2 56 -8.054238321195426 3 1 1.165431465126737 3 2 2.368262895680921 3 3 3.349820609756037 3 4 1.8574045078784494 3 5 1.693290703787838 3 6 1.544762344868417 3 7 1.6573431542801558 3 8 1.885299131157666 3 9 1.9909580723075209 3 10 1.8444011209339806 3 11 1.376320370489445 3 12 1.5596058545209246 3 13 -4.538215139179545 3 14 -6.489900053494445 3 15 -2.3042112051785786 3 16 2.910648045485047 3 17 2.7044741287354377 3 18 2.275554580212841 3 19 1.1626420541570184 3 20 1.3903607675097902 3 21 3.3017690706570733 3 22 3.446567006330092 3 23 3.2014260312530283 3 24 3.339297338410489 3 25 3.117895843942442 3 26 2.6873012480351974 3 27 2.4161220102992047 3 28 2.480732020758424 3 29 2.7856720381562 3 30 2.7490924072263354 3 31 2.8844923241207354 3 32 2.244748846156874 3 33 2.6712287217672923 3 34 3.1928552170803215 3 35 2.9225534147084815 3 36 4.707882319885151 3 37 5.081355746949367 3 38 6.229128355153712 3 39 8.092023304390139 3 40 10.932784612857692 3 41 10.428762258744179 3 42 8.030452168577757 3 43 2.718443764412786 3 44 4.497205361259002 3 45 8.028948246206937 3 46 2.422831782482156 3 47 -4.0523429704011065 3 48 1.8430100346963485 3 49 -9.611467806222826 3 50 1.7697061226939006 3 51 -0.09683622387382584 3 52 0.20514932806623776 3 53 -0.48846074652667326 3 54 -4.255502661798218 3 55 -0.6388203457784343 3 56 2.9690050186937214 4 1 0.9807057642614296 4 2 2.2523167957925025 4 3 3.4780089324388683 4 4 1.7677296407171 4 5 1.4326382533926392 4 6 1.44529525998432 4 7 1.622395470471261 4 8 1.7118559377243088 4 9 1.8643848803566456 4 10 1.6971823448441583 4 11 3.153533762810163 4 12 3.8221387228214496 4 13 4.485114526948742 4 14 3.2498766371260963 4 15 5.605286855884287 4 16 6.06421810860779 4 17 5.568678306228161 4 18 5.325998155971831 4 19 4.078436784305339 4 20 4.2729087727843265 4 21 3.003968613426828 4 22 3.495543700071657 4 23 2.948739998622706 4 24 3.4176896981398954 4 25 3.136965310169046 4 26 2.494749575555616 4 27 2.6429425435264235 4 28 1.9399831306731414 4 29 2.6310018135203848 4 30 2.495610513075917 4 31 3.020709485079827 4 32 3.7021351432941145 4 33 2.544015719574773 4 34 3.006319321384993 4 35 2.825637768227054 4 36 4.475490756017265 4 37 4.4163822366538845 4 38 5.452312893593046 4 39 7.821445992685511 4 40 10.572532433991714 4 41 5.9056315579959175 4 42 8.453850497268565 4 43 8.122561957651083 4 44 11.308125829724943 4 45 9.226977318560117 4 46 8.576511081499817 4 47 8.834894375790853 4 48 8.430098351917387 4 49 5.081750985472123 4 50 9.179769869641145 4 51 7.794723742731204 4 52 8.037136719122937 4 53 6.33867344977023 4 54 2.5112787025581085 4 55 7.6166433996083 4 56 6.8222309887474815 5 1 1.654325350440061 5 2 2.6562724138694733 5 3 3.913136237010285 5 4 2.157086922650379 5 5 1.9162093026655143 5 6 1.9111563941269125 5 7 2.018013174796728 5 8 2.111777682444405 5 9 2.2543477090986883 5 10 2.0172930201685944 5 11 3.384452051255394 5 12 5.588937988712395 5 13 6.034075315463852 5 14 5.9822901603837515 5 15 6.228282728849884 5 16 6.981491098425288 5 17 6.656883186003828 5 18 5.686363719922234 5 19 5.174886416785007 5 20 5.0451859082684365 5 21 3.433543519567074 5 22 3.958619318893909 5 23 3.4780624383242227 5 24 3.900692465023006 5 25 3.3602630249285497 5 26 3.2312994493223997 5 27 2.6989695974946173 5 28 2.8577778223566526 5 29 3.0909285889370466 5 30 2.8728382266243315 5 31 3.579619873468232 5 32 4.542913597007043 5 33 3.3348225874071957 5 34 3.105983010003513 5 35 2.882382162261017 5 36 4.1608206858669945 5 37 4.563092254261324 5 38 5.524866717388235 5 39 7.222392508928138 5 40 6.714006800729493 5 41 2.035162678443836 5 42 8.211054175266721 5 43 12.21585343062041 5 44 11.077496340526963 5 45 8.692223589789187 5 46 9.87114221033223 5 47 10.382345955336412 5 48 10.233724179886195 5 49 10.003579550512509 5 50 10.090195522467187 5 51 9.16393029008962 5 52 9.52347465150665 5 53 9.246341535590187 5 54 8.781417792002031 5 55 9.646687837427958 5 56 9.12306664334358 6 1 1.6118978895343332 6 2 2.7755325603035317 6 3 3.6805704772706065 6 4 2.1222523389027472 6 5 1.9264461281197356 6 6 1.7748800676331982 6 7 2.171396072744841 6 8 2.2150837477069913 6 9 2.38593498199031 6 10 2.0740362182161363 6 11 3.5145026704364497 6 12 5.829525984735177 6 13 5.858919390307925 6 14 6.6783942912704894 6 15 6.467582647497629 6 16 6.871744646287532 6 17 6.670340204895634 6 18 6.117338591114496 6 19 4.767390710376262 6 20 4.567862849423641 6 21 3.508749159990103 6 22 4.062207857265058 6 23 3.4963102011371987 6 24 3.8499150990282445 6 25 3.6287028694553527 6 26 2.7864382001133237 6 27 2.6101374543146463 6 28 2.6764413855854343 6 29 3.0088532025138335 6 30 2.7322005153344406 6 31 3.156418662223544 6 32 4.436805398507545 6 33 2.978386720101767 6 34 3.344797074904534 6 35 2.766059736155981 6 36 2.9462993083021374 6 37 2.8064484867479926 6 38 3.7535908428514944 6 39 5.476098556112447 6 40 1.483734722631329 6 41 0.6078825555115372 6 42 6.800722438848027 6 43 11.781308099335941 6 44 9.80405107251648 6 45 7.112328895764058 6 46 9.65015784804352 6 47 9.879057968352775 6 48 9.701714327224174 6 49 11.13549449221006 6 50 9.38290545648147 6 51 9.289529133079771 6 52 9.682021157879245 6 53 9.843613975253927 6 54 10.65877926718959 6 55 9.716921951140332 6 56 9.49806664334358 7 1 1.400271608080466 7 2 2.5938690001916953 7 3 3.487210738221215 7 4 2.1228348179335015 7 5 1.761348271642568 7 6 1.7731792288633912 7 7 1.8669925312723379 7 8 2.179832116765702 7 9 2.2021045998840663 7 10 2.115182536948666 7 11 3.1516415334373846 7 12 5.377359162740767 7 13 5.9460116549864 7 14 6.837946947374871 7 15 6.323104548709182 7 16 6.593715755327601 7 17 6.332572264541486 7 18 5.864659187573025 7 19 4.913313357160973 7 20 5.04221047290919 7 21 3.4603801012762148 7 22 3.8516766363890085 7 23 3.502903863765347 7 24 3.810725909839057 7 25 3.185692617824408 7 26 2.6346208655373715 7 27 2.733856000446991 7 28 2.2576855607951245 7 29 2.760297750510105 7 30 2.821506200329786 7 31 3.456162371450013 7 32 4.50765814780857 7 33 3.0951155178650467 7 34 3.0613860777004334 7 35 2.971022457498016 7 36 1.934975915944264 7 37 2.1461968558067035 7 38 2.404546108461929 7 39 4.978568267202846 7 40 -1.3255849418607504 7 41 -10.300784732466095 7 42 4.72010734099155 7 43 9.764556002411435 7 44 8.502257037101757 7 45 5.940683974981209 7 46 8.453093542358523 7 47 9.086071015883064 7 48 8.835707803459023 7 49 11.655788061641559 7 50 8.762355596276436 7 51 10.58990191965945 7 52 10.419299815847555 7 53 10.489024040491582 7 54 11.181752240162563 7 55 10.65830126148516 7 56 9.54720457437806 8 1 1.4593865898387364 8 2 2.583241719975149 8 3 3.576348080812714 8 4 1.701038889958923 8 5 1.6433772230371773 8 6 1.6050963760918293 8 7 1.8739950027328476 8 8 1.9894472750568681 8 9 2.2198755778763015 8 10 1.7428628947219948 8 11 2.940731800590491 8 12 5.20473353016456 8 13 5.9054326270942 8 14 6.37851420640747 8 15 5.892670277790032 8 16 6.131764478406339 8 17 5.841069381170886 8 18 5.209016019396017 8 19 4.058035023631497 8 20 4.118307919066659 8 21 2.7800340968040516 8 22 3.1474398469339064 8 23 2.692406041004375 8 24 3.098439220419266 8 25 2.0562233933922585 8 26 2.3085763792480876 8 27 1.911977690455224 8 28 1.7678420866277733 8 29 2.549670470293563 8 30 2.448751110298005 8 31 2.3115819312946684 8 32 3.615213755670169 8 33 1.870624521042636 8 34 2.3018121104178633 8 35 2.0296078405743927 8 36 0.3246546260772547 8 37 0.1776550175108369 8 38 1.0996267251501735 8 39 2.9022354438534315 8 40 -2.522583882663387 8 41 -10.067055180859645 8 42 4.549223499635776 8 43 9.266191873895487 8 44 7.258794638608169 8 45 5.073460248960259 8 46 8.363214761612376 8 47 8.655566131806331 8 48 8.066659904200463 8 49 11.000486190392884 8 50 7.9341691775404115 8 51 9.386983242479271 8 52 9.270435510798716 8 53 9.299533632112155 8 54 9.967076472244475 8 55 8.836375876055005 8 56 8.303954015357231 9 1 1.644010265067763 9 2 2.6962888429019145 9 3 3.7894490927281694 9 4 2.1519939225917 9 5 2.004421471884548 9 6 1.7816486783491783 9 7 1.9123040860212726 9 8 2.283824857237576 9 9 2.31317538028334 9 10 2.0747711636879274 9 11 3.591387449952805 9 12 5.801799574114028 9 13 6.091565194006357 9 14 6.928086088330705 9 15 6.561217071395205 9 16 6.897527007596203 9 17 6.3817555676267865 9 18 5.774036078075142 9 19 5.247796115781668 9 20 4.923517187972713 9 21 3.367639629406888 9 22 3.7554273925898585 9 23 3.4284937004259604 9 24 4.110725909839061 9 25 3.5759527509157607 9 26 3.0365567397055457 9 27 3.2076007070410597 9 28 3.153148355713455 9 29 3.0557000493606807 9 30 2.840320477401775 9 31 3.8442446461021618 9 32 4.763133041940449 9 33 3.317196582595834 9 34 3.6517672029272887 9 35 3.4718089063788398 9 36 2.6567545003362767 9 37 2.839663280489102 9 38 3.6556652857154077 9 39 4.99708006393606 9 40 0.6692728923800253 9 41 -2.8977599290783154 9 42 6.9286977826128435 9 43 11.248887520862734 9 44 10.046903135105385 9 45 7.333061470444001 9 46 9.815283500011276 9 47 10.692725583336177 9 48 10.774485782890366 9 49 12.637094776705084 9 50 10.072639927794885 9 51 10.055721641377541 9 52 9.68021935607744 9 53 10.115460822100776 9 54 10.253682064723971 9 55 9.934465810789458 9 56 10.51254032755411 10 1 1.45002530266175 10 2 2.5380808228517893 10 3 3.7346245313246627 10 4 1.9893372809876908 10 5 1.7776044292780355 10 6 1.6118491796023093 10 7 1.830662482011249 10 8 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9.448149789037199 114 42 5.648429696667645 114 43 2.3531650686648256 114 44 -0.23470538056581702 114 45 5.108874598770438 114 46 -2.429936291039386 114 47 -7.866273036809691 114 48 -3.3589066870485276 114 49 -14.189930652035308 114 50 -1.8260984912865226 114 51 -2.7472154766985497 114 52 -2.1441252132109483 114 53 -1.9841646460640163 114 54 -3.286787085680132 114 55 -1.0864488353765225 114 56 2.027561025366051 115 1 0.32534997798642706 115 2 1.1298016020725683 115 3 0.9846245313246627 115 4 0.6471294887798962 115 5 0.6959485851221885 115 6 0.5483751536282853 115 7 0.7592339105826795 115 8 1.0931550007782462 115 9 0.9869792080345405 115 10 0.8163580855380062 115 11 -0.29569388976010913 115 12 -3.1452897400805497 115 13 -0.7823582509697182 115 14 -6.802256814380616 115 15 -2.3618092443942658 115 16 -11.387161987619105 115 17 -9.5515578295024 115 18 -2.613005389229482 115 19 -2.543112975127425 115 20 -2.658181376620586 115 21 0.38067790691884795 115 22 0.41401989657710203 115 23 0.5942831741101742 115 24 0.9618622734754254 115 25 0.529501395253881 115 26 1.0333669470420688 115 27 1.4940440882212869 115 28 1.887897159541204 115 29 1.812139443300076 115 30 1.6468196799536088 115 31 0.40365453445942023 115 32 -5.648728999526856 115 33 0.3989948282098723 115 34 0.9823892124966704 115 35 1.0205130531092976 115 36 3.1185966055994356 115 37 3.275428830728334 115 38 4.427116641377289 115 39 5.943850398864292 115 40 8.807291233687838 115 41 8.566574201703183 115 42 3.23499762312321 115 43 0.9281139961418425 115 44 0.6369749054403115 115 45 3.921757642692807 115 46 -2.4235522256665583 115 47 -5.764830238035433 115 48 -4.195091569581727 115 49 -8.388921969706402 115 50 -0.9533967548207407 115 51 1.8699959635466001 115 52 2.4332496591077444 115 53 2.004854761494716 115 54 2.285161331071656 115 55 3.1316946784130586 115 56 5.412839370616307 116 1 0.5179789706154168 116 2 1.6586406684116355 116 3 2.156921828621961 116 4 1.082387474037887 116 5 0.9818515335251377 116 6 0.5228530406061722 116 7 0.8409906673394332 116 8 0.7495432071664467 116 9 0.7197187657740969 116 10 1.09430648848641 116 11 -1.0490108430770633 116 12 -2.561690231481041 116 13 -6.35594547455694 116 14 -9.235119222243025 116 15 -6.4709308660158875 116 16 -9.02082292128004 116 17 -11.714119254563826 116 18 -3.618472219696315 116 19 -4.6603119923264416 116 20 -4.5220020154412275 116 21 0.995235646476587 116 22 1.2027483978055997 116 23 1.165904795731798 116 24 1.3918069909201378 116 25 1.2868109775634622 116 26 1.0918436055187328 116 27 1.3952725894497817 116 28 1.7460359801800251 116 29 1.6520964457570813 116 30 1.592335650469579 116 31 0.4922294730343566 116 32 -9.540897304195159 116 33 0.7229813146963622 116 34 0.6474997776072371 116 35 0.5180867631830068 116 36 2.6314344434372714 116 37 2.8942863245858312 116 38 3.8637259779866255 116 39 6.0747472047610955 116 40 8.956707695604297 116 41 7.115990663619645 116 42 3.808830546956134 116 43 -9.755854062826218 116 44 0.7871591806245881 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0.7337273913506337 118 9 0.9130454257674216 118 10 0.7087542696008597 118 11 -6.527455954855508 118 12 -8.970065273189416 118 13 -9.73325611853425 118 14 -1.1814655685893705 118 15 -16.83943270118439 118 16 -6.387723154846938 118 17 -14.03507354218478 118 18 -3.213033447590874 118 19 -3.43985820520599 118 20 -3.4762229029954455 118 21 0.7046116891859668 118 22 0.6885008168913558 118 23 0.19613502596202892 118 24 -2.083101250654771 118 25 0.7507415748273871 118 26 1.130448877457333 118 27 1.8711484653256605 118 28 1.348405015882399 118 29 1.2137247407187104 118 30 1.7120553701892973 118 31 -1.3990951849569697 118 32 -8.222312052276575 118 33 -0.18819652981481738 118 34 0.29608169285581454 118 35 -0.21454586944962273 118 36 2.623226235229062 118 37 2.6939192908854643 118 38 3.6796418939025415 118 39 5.702408199088758 118 40 8.05146912369906 118 41 8.193468141097124 118 42 2.919341057466646 118 43 0.677412537107049 118 44 1.4895282163269563 118 45 2.507265499033995 118 46 -5.868277253724919 118 47 -8.65743685980872 118 48 -7.73142714758397 118 49 -4.715731734016165 118 50 -4.420147596571585 118 51 -3.8766370330863964 118 52 -3.4901510142929304 118 53 -3.715403375430089 118 54 -8.204667512923853 118 55 -5.227522493304111 118 56 -1.9510074307304919 119 1 0.1363072326522925 119 2 1.1850770385566207 119 3 2.1775053260266475 119 4 0.744209561025535 119 5 0.5816725465911858 119 6 0.021546435988305745 119 7 0.18224715561579075 119 8 0.25886842642544394 119 9 0.4035204482512782 119 10 0.6239288260557672 119 11 -8.094008158273056 119 12 -10.511108523945694 119 13 -2.237957287694581 119 14 -2.050977464591334 119 15 -8.426792688102875 119 16 -11.18577727961188 119 17 -12.670876011320583 119 18 -2.507571915418527 119 19 -2.760150904085883 119 20 -2.6823385108770594 119 21 -0.5474527372713993 119 22 -0.11642110282085127 119 23 -0.6424717927772434 119 24 -5.797883361684121 119 25 -0.8035058293096355 119 26 1.7203025280173847 119 27 1.25623553915446 119 28 1.336836051774803 119 29 1.7210422127160925 119 30 1.7093573078885882 119 31 -1.3996266214707447 119 32 -9.780238632278209 119 33 -0.35555815192257967 119 34 -0.10821885493258776 119 35 -0.3711561220863686 119 36 2.450550247983543 119 37 2.3974185959300236 119 38 3.270885455344782 119 39 5.496905787184581 119 40 7.774141022971403 119 41 6.7188544545629085 119 42 1.8516442215578905 119 43 -1.3218107479882022 119 44 -0.3664116087077893 119 45 0.6480144157211036 119 46 -7.11093934186162 119 47 -9.399199593845186 119 48 -10.241524441345723 119 49 -6.162138405588401 119 50 -6.256301631401115 119 51 -6.111491092443767 119 52 -6.0226504010969535 119 53 -9.110587743020636 119 54 -8.846724581029488 119 55 -9.938707187932515 119 56 -6.002761171225956 120 1 -0.02022910046936488 120 2 0.8847050889966042 120 3 2.3938711066671274 120 4 0.5689850304984532 120 5 0.3650799674385041 120 6 -0.028757474018043183 120 7 0.2335489790758274 120 8 0.38923221123901897 120 9 0.34641258287886956 120 10 0.6035623196600497 120 11 -9.072779817530972 120 12 -2.4253333266309873 120 13 -7.617663356822767 120 14 -6.830121073409259 120 15 -10.172596915627143 120 16 -12.3659913774074 120 17 -11.390054093512365 120 18 -3.717768776527116 120 19 -3.5069984047662786 120 20 -4.918299682971771 120 21 -0.7903993035419248 120 22 -0.3922534533606381 120 23 -0.7584565519172202 120 24 -6.911191898380121 120 25 -1.5618746944098802 120 26 0.8982486406908876 120 27 1.1646542999273848 120 28 0.7793199490804312 120 29 0.8031886338355676 120 30 0.9956677496920889 120 31 -3.3357601604347273 120 32 -10.976328625927852 120 33 -3.4922722950778002 120 34 -11.201296964340191 120 35 -3.528599649256826 120 36 -1.5064033944005644 120 37 -1.4937181182131347 120 38 -0.9010278169041541 120 39 0.5987383191631714 120 40 -2.485906151119135 120 41 0.4994042141564847 120 42 -4.4558492012852575 120 43 -5.3245323301346215 120 44 -6.189145891570895 120 45 -10.109764773247418 120 46 -13.813651852067553 120 47 -13.252143438533563 120 48 -11.377376750154578 120 49 -15.78828373807502 120 50 -10.623851300275287 120 51 -10.876890711422268 120 52 -10.521607127940824 120 53 -11.634310867396941 120 54 -11.46071351326209 120 55 -11.447461610503504 120 56 -7.931727877204365 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1453.0 5 35.0 6 197.0 7 236.0 8 436.0 9 543.0 10 466.0 11 314.0 12 297.0 13 329.0 14 390.0 15 604.0 16 807.0 17 1114.0 18 1500.0 19 1814.0 20 2108.0 21 2975.0 22 3788.0 23 4640.0 24 5334.0 25 5992.0 26 6341.0 27 6731.0 28 7162.0 29 7520.0 30 8445.0 31 9638.0 32 11516.0 33 13992.0 34 16988.0 35 20164.0 36 24370.0 37 25856.0 38 16414.0 39 4238.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 100.0 0.0 7 99.68055432671935 0.2188621959794549 0.07078096550824924 0.02980251179294705 8 99.64795782944582 0.24307673681122438 0.06333033756001248 0.04563509618295017 9 97.90349851647251 1.0890069914435165 0.4136177110358608 0.593876781048111 10 49.95459773594043 23.984037029620904 17.170903435205148 8.890461799233517 11 37.14650262867467 29.790404522531166 16.85564874014538 16.207444108648783 12 34.505720685271505 11.17268227262779 18.757421523932813 35.56417551816789 13 25.39174004759089 10.571975394301202 21.7260310970584 42.31025346104951 14 21.987051096930458 13.65922958684615 33.30429922213424 31.049420094089154 15 33.89553812140265 15.09137382576005 24.162056773976882 26.851031278860415 16 39.63394466778779 15.295317864413255 20.922638318884598 24.148099148914365 17 30.394339692949767 18.277686689521538 29.672361752869282 21.655611864659413 18 28.969477380475585 19.212857315835063 30.783417005858627 21.034248297830725 19 36.2618067328651 23.25111313970602 19.022113754494477 21.464966372934406 20 27.49080068936606 32.14076109739625 19.474591271135125 20.893846942102567 21 24.82221611627788 22.3697997922944 30.737168590815315 22.070815500612404 22 23.853052937451107 19.524643296203852 25.11082963901203 31.51147412733301 23 24.92514447233256 20.500402799573454 22.199611636018197 32.37484109207579 24 26.034822372373075 21.309261596203903 29.438362351976977 23.217553679446045 25 34.46042227417087 19.759059708859933 20.34394762086597 25.43657039610323 26 23.938207725434026 19.064904283561408 24.249875193042534 32.747012797962036 27 22.64827676155075 17.323681893768953 23.79601552055822 36.232025824122076 28 22.12342315443756 29.38624318877361 23.690471747406136 24.7998619093827 29 35.78375206103457 21.251711706676353 21.709098361419297 21.25543787086978 30 26.67607654610841 19.18357649952485 33.6789833603518 20.461363594014944 31 26.339987957262284 26.016047648165348 27.298272473942408 20.345691920629957 32 36.81627026448028 17.20914853089795 23.761776800808484 22.212804403813287 33 28.007950282738932 18.87818897784745 25.7810312972398 27.33282944217382 34 22.821522615524433 27.186633421107825 31.05119660708876 18.940647356278983 35 22.52957428865624 23.475392421341013 37.395226180991386 16.599807109011362 36 22.89068950557678 32.40270372391788 25.263211908327154 19.443394862178188 37 24.48988166094811 22.89102070349882 33.94300119039283 18.676096445160237 38 33.61577310309129 24.21551848760265 23.19798919049995 18.970719218806106 39 23.46220373054088 18.094525735122247 21.69604039081857 36.7472301435183 40 15.940983268597344 14.024673079355857 24.086004376121554 45.94833927592524 41 17.14922048997773 16.151222125116295 26.69504665783316 40.00451072707282 42 26.59107347005436 21.021908028277455 28.564429201371876 23.822589300296308 43 13.006621773812919 11.567776214887541 27.74182091770215 47.683781093597396 44 14.952282439282389 12.921887359409883 20.084904026622606 52.04092617468512 45 21.729058000400872 20.229521798511186 35.466338512023796 22.575081689064152 46 20.041097039335735 21.53121417987643 28.3988928960832 30.028795884704635 47 17.814172524140243 25.126814885202837 28.26865657737222 28.7903560132847 48 21.77048538804489 27.729871001104105 29.120953352620276 21.378690258230726 49 15.711375656431628 17.026574968696856 20.03728344764432 47.224765927227196 50 28.16951367497433 19.454401194810046 29.86698403808457 22.509101092131058 51 20.946173254835998 27.629660779366418 30.764881786748905 20.659284179048687 52 20.219455522153602 20.17971602623742 36.53602502185672 23.06480342975226 53 20.630018241790026 18.547841543693984 37.557093070452495 23.265047144063487 54 18.471860240671756 23.01720882307689 27.922492691728486 30.588438244522877 55 20.597233492831894 27.264459803564932 29.142998386359608 22.995308317243566 56 26.66514279316683 20.259276191187034 27.71848147560714 25.357099540038995 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 0.5 5 0.0 6 0.5 7 1.0 8 4.0 9 7.0 10 24.0 11 41.0 12 90.5 13 185.5 14 231.0 15 319.5 16 408.0 17 474.0 18 540.0 19 607.0 20 674.0 21 783.5 22 1086.5 23 1280.0 24 1595.0 25 1910.0 26 2298.0 27 2686.0 28 2986.5 29 3433.0 30 3579.0 31 3507.0 32 3435.0 33 3352.0 34 3269.0 35 3104.0 36 2939.0 37 2795.5 38 2782.5 39 2913.0 40 3155.0 41 3397.0 42 3792.5 43 4188.0 44 6166.5 45 8145.0 46 10826.0 47 12122.5 48 10738.0 49 9966.5 50 9195.0 51 9717.0 52 10239.0 53 10877.5 54 11840.0 55 12164.0 56 13022.0 57 13880.0 58 15354.5 59 16829.0 60 16801.5 61 16774.0 62 15322.5 63 12416.0 64 10961.0 65 9640.0 66 8319.0 67 7393.5 68 6468.0 69 5698.0 70 4928.0 71 4312.5 72 2979.5 73 2262.0 74 1804.0 75 1346.0 76 1043.0 77 740.0 78 573.5 79 307.5 80 208.0 81 130.0 82 52.0 83 35.5 84 19.0 85 12.0 86 5.0 87 4.0 88 2.0 89 1.0 90 1.0 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.026077197818828668 10 0.0 11 0.0 12 0.0 13 0.0 14 0.026542862065593465 15 0.16437947910797357 16 0.03725313974118381 17 0.02747419055912306 18 0.00977894918206075 19 0.01816090562382711 20 0.026542862065593465 21 0.009313284935295953 22 0.001396992740294393 23 0.0 24 0.0 25 0.0023283212338239883 26 0.1937163266541558 27 0.02980251179294705 28 0.18393737747209507 29 0.022817548091475084 30 0.03678747549441901 31 0.2374887658500468 32 0.011175941922355144 33 0.19325066240739103 34 0.08475089291119317 35 0.05494838111824612 36 0.17508975678356392 37 0.24773337927887235 38 0.1424932595100281 39 0.38976097454213565 40 0.6128141487424738 41 0.8945410180351763 42 0.20582359707004058 43 0.352042170554187 44 0.5094366859606886 45 0.0996521488076667 46 0.061933344819718086 47 0.02980251179294705 48 0.04377243919589098 49 0.3310872794497711 50 0.22677848817445645 51 0.3385379073980079 52 0.39767726673713716 53 0.18812835569297826 54 0.12293536114590657 55 0.15134088019855924 56 0.1774180780173879 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 214747.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.83049091100524 #Duplication Level Percentage of deduplicated Percentage of total 1 89.02901217974028 61.27910613653389 2 6.841787409551296 9.418471722163014 3 1.6733518958352696 3.4553289737160875 4 0.7201251750269119 1.982662772579037 5 0.4344901473267666 1.4953085068248173 6 0.26688807981482016 1.1022022531169773 7 0.17037172700951406 0.8208738725194438 8 0.1356212486591231 0.7467901698536765 9 0.09600236393922908 0.5947100854698714 >10 0.554172551010654 7.11790933588374 >50 0.039583403587906664 1.8486038509498364 >100 0.032656307960023 4.972975945388635 >500 0.001979170179395333 0.9910532804465777 >1k 0.003958340358790666 4.174003094554384 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 2068 0.9629936623096016 No Hit TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 1546 0.7199169254983772 No Hit TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 1454 0.6770758147960158 No Hit TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 1060 0.49360410157068557 No Hit TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG 849 0.3953489455033132 No Hit TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAACGATTAAG 606 0.2821925335394674 No Hit TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 490 0.22817548091475084 No Hit TCACTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC 478 0.2225875099535733 No Hit TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 391 0.1820747204850359 No Hit TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTCAG 376 0.17508975678356392 No Hit TCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 375 0.1746240925367991 No Hit TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGCCTCAAAGATTAAG 335 0.1559975226662072 No Hit TCACTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 333 0.1550661941726776 No Hit TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG 297 0.1383022812891449 No Hit TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC 273 0.12712633936678977 No Hit TCACTTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGCGCTAATACATGCCGAC 272 0.12666067512002496 No Hit TCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGACCACCCGACTGCTCTTC 269 0.12526368237973057 No Hit TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG 267 0.12433235388620097 No Hit TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTCAG 248 0.11548473319766982 No Hit TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAAAGATTAAG 225 0.10477445552207947 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 4.6566424676479763E-4 0.0 0.0 0.0 7 0.0 4.6566424676479763E-4 0.0 0.0 0.0 8 4.6566424676479763E-4 4.6566424676479763E-4 0.0 0.0 0.0 9 9.313284935295953E-4 4.6566424676479763E-4 0.0 0.0 0.0 10 0.006053635207942369 4.6566424676479763E-4 0.0 0.0 0.0 11 0.016298248636767917 4.6566424676479763E-4 0.0 0.0 0.0 12 0.01769524137706231 4.6566424676479763E-4 0.0 0.0 0.0 13 0.0195578983641215 4.6566424676479763E-4 0.0 0.0 0.0 14 0.026542862065593465 4.6566424676479763E-4 0.0 0.0 0.0 15 0.028405519052652657 4.6566424676479763E-4 0.0 0.0 0.0 16 0.03166516878000624 4.6566424676479763E-4 0.0 0.0 0.0 17 0.03399349001383023 4.6566424676479763E-4 0.0 0.0 0.0 18 0.03818446823471341 4.6566424676479763E-4 0.0 0.0 0.0 19 0.04144411796206699 4.6566424676479763E-4 0.0 0.0 0.0 20 0.04796341741677416 4.6566424676479763E-4 0.0 0.0 0.0 21 0.05029173865059815 4.6566424676479763E-4 0.0 0.0 0.0 22 0.05494838111824612 4.6566424676479763E-4 0.0 0.0 0.0 23 0.05727670235207011 4.6566424676479763E-4 0.0 0.0 0.0 24 0.06100201632618849 4.6566424676479763E-4 0.0 0.0 0.0 25 0.06472733030030688 4.6566424676479763E-4 0.0 0.0 0.0 26 0.06752131578089565 4.6566424676479763E-4 0.0 0.0 0.0 27 0.07124662975501404 4.6566424676479763E-4 0.0 0.0 0.0 28 0.07776592920972121 4.6566424676479763E-4 0.0 0.0 0.0 29 0.0786972577032508 4.6566424676479763E-4 0.0 0.0 0.0 30 0.0796285861967804 4.6566424676479763E-4 0.0 0.0 0.0 31 0.08195690743060438 4.6566424676479763E-4 0.0 0.0 0.0 32 0.08195690743060438 4.6566424676479763E-4 0.0 0.0 0.0 33 0.08242257167736919 4.6566424676479763E-4 0.0 0.0 0.0 34 0.08288823592413398 4.6566424676479763E-4 0.0 0.0 0.0 35 0.08335390017089878 4.6566424676479763E-4 0.0 0.0 0.0 36 0.08475089291119317 4.6566424676479763E-4 0.0 0.0 0.0 37 0.08521655715795796 4.6566424676479763E-4 0.0 0.0 0.0 38 0.08568222140472277 4.6566424676479763E-4 0.0 0.0 0.0 39 0.08568222140472277 4.6566424676479763E-4 0.0 0.0 0.0 40 0.08568222140472277 4.6566424676479763E-4 0.0 0.0 4.6566424676479763E-4 41 0.08754487839178196 4.6566424676479763E-4 0.0 0.0 4.6566424676479763E-4 42 0.09033886387237074 4.6566424676479763E-4 0.0 0.0 4.6566424676479763E-4 43 0.09033886387237074 4.6566424676479763E-4 0.0 0.0 4.6566424676479763E-4 44 0.09173585661266513 4.6566424676479763E-4 0.0 0.0 4.6566424676479763E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACCGCG 15 0.007974624 50.69452 38 ATGCGTC 15 0.007974624 50.69452 38 TTCGACC 15 0.007974624 50.69452 38 TACCGCT 15 0.007974624 50.69452 38 CGCGAAT 15 0.008042199 50.586452 41 AAGACTA 20 4.007553E-4 50.419262 36 CTGCAAT 15 0.008270525 50.22954 49 CGATTGT 25 2.0345211E-5 50.194126 44 CGTGCAA 35 5.113361E-8 50.182335 48 ACACGAT 15 0.00830133 50.18233 46 ACCGAAT 15 0.00830133 50.18233 42 ATATGCG 15 0.00830133 50.18233 34 TATTGTC 15 0.00830133 50.18233 42 TACATCC 15 0.00830133 50.18233 34 CTACGAA 15 0.00830133 50.18233 46 ACGATTA 575 0.0 50.182327 48 CACGATT 225 0.0 50.17054 47 TCGCATA 25 2.103383E-5 49.912598 30 CGAGTAA 25 2.103383E-5 49.912598 31 TAGCATT 15 0.008480112 49.912598 30 >>END_MODULE