FastQCFastQC Report
Fri 17 Jun 2016
SRR1526321_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526321_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49861
Sequences flagged as poor quality0
Sequence length56
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG2550.5114217524718718No Hit
ACGAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG2510.503399450472313No Hit
ACGAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG1800.3610035899801448No Hit
ACGAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG1750.35097571248069637No Hit
ACGAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG1710.34295341048113753No Hit
ACGAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAACGATTAAG1040.2085798519885281No Hit
ACGAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC790.15844046449128577No Hit
ACGAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGCCTCAAAGATTAAG710.14239586049216824No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC670.13437355849260946No Hit
ACGAGCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGCGCTAATACATGCCGAC590.11832895449349191No Hit
ACGAGCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACCACCCATTCGAACGTC540.10830107699404344No Hit
ACGAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC530.10629550149415376No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGTC203.8599322E-450.7256938
AATTCCC150.00804807650.5188635
CTCGCCT150.00804807650.5188641
AACGATT505.456968E-1250.21175447
CCTGCCG150.00824470150.21175448
GCGTGTT150.00824470150.21175448
AAGATTC204.059254E-450.21175448
TATCTCA150.00824470150.21175448
TTTGGTA150.00824470150.21175446
TTGGTAT150.00824470150.21175447
ACGATTC252.0074782E-550.21175448
TACCAAG204.059254E-450.21175447
CGATTCA252.0074782E-550.21175449
GTTTGGT150.00827781650.1609345
AATTATC150.00827781650.1609345
TCAACGA507.2759576E-1250.1609345
AATCACT150.008311029550.11021450
CAAACAA150.008311029550.11021450
ATGATTT252.0439331E-550.05959733
GTGTGGC204.120618E-450.05959733