##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526321_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	49861
Sequences flagged as poor quality	0
Sequence length	56
%GC	56
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.64220533081968	38.0	37.0	39.0	33.0	40.0
2	36.118409177513485	39.0	38.0	39.0	30.0	40.0
3	35.454262850725016	38.0	35.0	39.0	27.0	40.0
4	35.910671667234915	38.0	36.0	39.0	30.0	40.0
5	35.194159764144324	38.0	35.0	39.0	27.0	40.0
6	35.488217243938145	38.0	36.0	39.0	28.0	40.0
7	35.67830568981769	38.0	36.0	39.0	30.0	40.0
8	35.72710134173001	38.0	36.0	39.0	30.0	40.0
9	35.909147429855	38.0	36.0	39.0	30.0	40.0
10	34.79729648422615	38.0	35.0	39.0	26.0	40.0
11	33.58302079781794	36.0	31.0	39.0	24.0	40.0
12	32.02041675858888	36.0	29.0	39.0	15.0	40.0
13	31.793064719921382	36.0	29.0	39.0	13.0	40.0
14	31.217885722308015	36.0	28.0	39.0	11.0	40.0
15	31.744610015844046	36.0	29.0	39.0	13.0	40.0
16	31.6709051162231	36.0	29.0	38.0	14.0	40.0
17	31.79675497884118	36.0	29.0	38.0	16.0	40.0
18	32.075489861815846	36.0	29.0	38.0	18.0	40.0
19	32.757285253003346	36.0	30.0	38.0	23.0	40.0
20	32.74493090792403	36.0	30.0	38.0	22.0	40.0
21	34.142997533142136	38.0	33.0	39.0	24.0	40.0
22	33.90710174284511	38.0	33.0	39.0	23.0	40.0
23	34.063677022121496	38.0	33.0	39.0	24.0	40.0
24	33.68057199013257	38.0	33.0	39.0	23.0	40.0
25	33.81761296404003	38.0	33.0	39.0	23.0	40.0
26	34.092316640259924	38.0	34.0	39.0	24.0	40.0
27	34.02013597801889	38.0	33.0	39.0	24.0	40.0
28	33.68707005475221	38.0	33.0	39.0	23.0	40.0
29	33.643849902729585	38.0	33.0	39.0	23.0	40.0
30	33.243436754176614	37.0	33.0	39.0	21.0	40.0
31	32.9053970036702	37.0	32.0	39.0	18.0	40.0
32	31.87398969134193	36.0	31.0	39.0	10.0	40.0
33	33.02779727642847	37.0	33.0	39.0	19.0	40.0
34	32.67034355508313	37.0	32.0	39.0	17.0	40.0
35	32.73408074446962	37.0	32.0	39.0	17.0	40.0
36	30.796855257616173	35.0	30.0	38.0	4.0	39.0
37	30.54292934357514	35.0	30.0	38.0	4.0	39.0
38	29.936824371753474	34.0	29.0	38.0	4.0	39.0
39	28.021359379073825	33.0	23.0	37.0	4.0	39.0
40	24.200096267623994	29.0	10.0	36.0	4.0	38.0
41	20.93034636288883	23.0	7.0	33.0	4.0	36.0
42	24.29670483945368	29.0	16.0	33.0	4.0	36.0
43	20.695393193076754	24.0	6.0	32.0	4.0	36.0
44	21.804075329415777	26.0	7.0	33.0	4.0	36.0
45	23.860111108882695	29.0	11.0	33.0	4.0	36.0
46	23.739375463789333	29.0	4.0	35.0	4.0	38.0
47	23.25075710475121	29.0	4.0	35.0	4.0	38.0
48	23.161368604721126	28.0	4.0	34.0	4.0	38.0
49	21.025510920358595	25.0	4.0	34.0	4.0	38.0
50	22.88413790337137	28.0	4.0	34.0	4.0	38.0
51	24.293435751388863	31.0	4.0	36.0	4.0	39.0
52	24.189566996249575	31.0	4.0	36.0	4.0	39.0
53	23.819698762559916	30.0	4.0	36.0	4.0	39.0
54	22.94237981588817	29.0	4.0	36.0	4.0	39.0
55	23.090792402880005	30.0	4.0	36.0	4.0	39.0
56	22.5599967910792	29.0	4.0	36.0	4.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	2.451521455141979
1	2	3.3309398736305766
1	3	3.9006592961416686
1	4	3.305276260864673
1	5	4.205411670625253
1	6	4.05007671588411
1	7	3.8119369016984876
1	8	3.6264815769633714
1	9	3.719566304787861
1	10	3.610150402628612
1	11	-4.777407062235863
1	12	-7.5631825153329295
1	13	-19.264875266594093
1	14	-17.009910709679325
1	15	-10.21246166318786
1	16	-20.177915869527144
1	17	-19.67946199675471
1	18	-19.492848860688575
1	19	-6.101746526881595
1	20	-9.63233263111773
1	21	-6.679238115932982
1	22	-2.1189753025180273
1	23	-2.029989145828935
1	24	-2.5024962342368724
1	25	-2.2205140537449815
1	26	3.964835053046329
1	27	3.2266049515238535
1	28	3.8110034942846625
1	29	3.0339320760649926
1	30	3.2497988069609036
1	31	1.6124153743628504
1	32	-11.608957444463403
1	33	1.581831929473914
1	34	1.945586694471352
1	35	2.2549476436652007
1	36	5.47292698007233
1	37	5.534151010691705
1	38	6.462404131963876
1	39	7.73176951941349
1	40	11.100150529848118
1	41	-1.6066738329223291
1	42	-6.435671030346207
1	43	-12.825246229069428
1	44	-14.749896592482047
1	45	-14.803870846240683
1	46	-17.017516458174086
1	47	-16.09247948079485
1	48	-15.94225718764481
1	49	-13.28562033007878
1	50	-15.04259592538293
1	51	-16.427427733977428
1	52	-15.976057397619762
1	53	-16.208938012040285
1	54	-16.40494041728981
1	55	-15.959224162248116
1	56	-15.57551522328428
2	1	2.10766421423601
2	2	2.8206447466573437
2	3	3.6618123503626663
2	4	2.616189095456299
2	5	3.8440527138647838
2	6	3.2443114447791004
2	7	3.022300662851542
2	8	3.27610408897435
2	9	3.0603350076018643
2	10	3.646869688833142
2	11	-2.820646595523442
2	12	-9.51273639316409
2	13	-11.775513564466433
2	14	-13.68012347563677
2	15	-5.93277738041504
2	16	-2.919508182499005
2	17	-1.6019053735563595
2	18	-9.807193404271278
2	19	-1.4294747355294994
2	20	-1.9449613202048965
2	21	3.7833562560642733
2	22	4.227627305580803
2	23	3.207828287252738
2	24	3.024271095893532
2	25	3.3995957609427307
2	26	4.160099294638641
2	27	3.6614024806933756
2	28	3.592403631553026
2	29	4.063787944287363
2	30	2.482811847455075
2	31	-0.4366580642095599
2	32	-8.26990459614494
2	33	-0.02386470745127056
2	34	0.4074947246703893
2	35	0.8616737932877108
2	36	5.846296918301569
2	37	6.6518586290856625
2	38	5.553687591126536
2	39	7.804521750024328
2	40	11.132408594364247
2	41	7.718652179431825
2	42	4.217040019756745
2	43	0.30927676338081156
2	44	-8.716609015268595
2	45	1.2863831002246577
2	46	-10.472217899491863
2	47	-10.90304914448737
2	48	-8.51397990555833
2	49	-10.590699259385575
2	50	-8.560440812136532
2	51	-8.59043391105361
2	52	-8.45821251086616
2	53	-8.272767799274327
2	54	-13.253945221682399
2	55	-7.3085721375398105
2	56	-7.805439725686476
3	1	2.2969115260639654
3	2	3.261720015474552
3	3	4.260091920255142
3	4	3.590382643843398
3	5	4.391794649348654
3	6	3.932913595316734
3	7	3.361440447797783
3	8	3.5115879599420907
3	9	3.5680769430857353
3	10	3.8952567856073372
3	11	0.9238695335088174
3	12	1.7519947896316097
3	13	-4.442180231133101
3	14	-5.393456808970104
3	15	-2.349766627726865
3	16	3.8501692368558373
3	17	4.014438712465147
3	18	3.554527025836247
3	19	2.436976877373727
3	20	2.2888021206553155
3	21	3.979485288322337
3	22	5.020315477623818
3	23	4.312989577575323
3	24	4.341475396968804
3	25	3.689273180297569
3	26	3.4252605849612223
3	27	3.3437680720912297
3	28	2.600790728327219
3	29	3.810669664717473
3	30	3.279586041003462
3	31	3.742911828263562
3	32	2.645794328586245
3	33	4.011619163516471
3	34	4.428139885960711
3	35	4.429415728771584
3	36	5.949522724753187
3	37	6.127342500053405
3	38	6.466375763169548
3	39	8.619145405938312
3	40	11.886817196514787
3	41	9.631340351474833
3	42	6.608867976745991
3	43	1.8641154730582308
3	44	3.694358726666888
3	45	7.302512132482722
3	46	0.5297175843791067
3	47	-3.4161674240572637
3	48	0.6012889116459732
3	49	-9.173279904546865
3	50	-0.00968812396448726
3	51	-1.1945199325589861
3	52	-0.7289651990382033
3	53	-1.0461011326076601
3	54	-3.887493608779174
3	55	-2.323195793453788
3	56	0.3541301667866428
4	1	2.490485823252719
4	2	3.5654951158761534
4	3	3.6917385066005224
4	4	3.2096597522771333
4	5	3.7696259746498626
4	6	3.4440782539512753
4	7	3.290275789163239
4	8	3.603154225002335
4	9	3.8413299551339293
4	10	3.8759796771735964
4	11	4.533026160014838
4	12	3.8236413759769903
4	13	5.995570772882967
4	14	5.422286162917445
4	15	6.710634978698835
4	16	6.940771646494387
4	17	6.758374455437032
4	18	5.958061162382432
4	19	5.262519046048421
4	20	5.7614125624223895
4	21	4.118762396756075
4	22	5.177102626218193
4	23	3.9553992161295355
4	24	4.622599894960771
4	25	3.77794787909275
4	26	3.6064654042383353
4	27	3.3747720881554883
4	28	3.0383810897730044
4	29	4.094685728974497
4	30	3.8265739928106868
4	31	3.7967270893077334
4	32	4.148284288425597
4	33	3.3393300068899663
4	34	3.7953688016233613
4	35	4.202909704675196
4	36	4.978438387403784
4	37	5.135535271137744
4	38	5.792319538269954
4	39	7.8694265304363
4	40	9.437499927438477
4	41	5.403067259105356
4	42	7.994731430561252
4	43	6.206284147757028
4	44	9.07604547365484
4	45	8.543475987904408
4	46	7.793813969921274
4	47	8.495318519717838
4	48	8.468919433734325
4	49	3.85178033641699
4	50	7.580833964388926
4	51	6.316484083505269
4	52	7.027018736704768
4	53	5.510364730846156
4	54	2.5163618129075758
4	55	6.060258021807254
4	56	6.385776753132024
5	1	2.5969115260639626
5	2	3.929412323166858
5	3	4.228809868973087
5	4	3.813459566920315
5	5	4.5553843929384
5	6	4.229836672239813
5	7	3.299389165746497
5	8	4.032100780454911
5	9	4.040897455906247
5	10	3.95166704201759
5	11	5.34643363607292
5	12	7.0017383793751975
5	13	7.198845409892538
5	14	6.717312421799129
5	15	7.648694910734669
5	16	7.4824769291635285
5	17	7.261618199644634
5	18	6.638629589938812
5	19	6.2487717491685935
5	20	6.275468787321987
5	21	4.927690416527469
5	22	4.429033426341768
5	23	3.6222203468060883
5	24	5.200449755943161
5	25	4.481068052092439
5	26	3.1257734054740425
5	27	4.175050123373282
5	28	3.7177138052502983
5	29	4.34707992112773
5	30	4.528303989721408
5	31	4.919834905186637
5	32	6.0898968926888095
5	33	4.8346960865933895
5	34	4.731729629550458
5	35	5.085825985181838
5	36	5.493112468342929
5	37	5.459137371848275
5	38	7.524324481118267
5	39	7.63247873927164
5	40	6.856560786258374
5	41	1.5482634283979095
5	42	7.2596372075152225
5	43	11.61283342177618
5	44	11.24410231641048
5	45	9.238409568380156
5	46	10.28253809719962
5	47	10.944345396455557
5	48	10.022827373184434
5	49	9.907232915965956
5	50	10.193901619625255
5	51	8.78342878538973
5	52	9.031034800961798
5	53	9.740052713546188
5	54	7.231993570708006
5	55	9.286547796289799
5	56	8.486950679607155
6	1	3.110244859397298
6	2	4.128386682141219
6	3	4.566758586921807
6	4	4.097049310510059
6	5	4.698461316015319
6	6	3.946246928650069
6	7	3.641440447797777
6	8	4.138254626608763
6	9	4.274743609752406
6	10	4.9752567856073355
6	11	6.630536200175481
6	12	8.725328122964939
6	13	8.3178197688669
6	14	8.713209857696562
6	15	8.2769000389398
6	16	8.170169236855838
6	17	7.9744387124651475
6	18	7.1545270258362486
6	19	5.876976877373725
6	20	6.102135453988648
6	21	4.592818621655674
6	22	5.460315477623816
6	23	4.939656244241988
6	24	5.114808730302137
6	25	4.662606513630905
6	26	4.505260584961228
6	27	4.397101405424564
6	28	3.854124061660549
6	29	5.1440029980508015
6	30	4.1595860410034575
6	31	4.542911828263559
6	32	5.912460995252911
6	33	5.1316191635164685
6	34	4.881473219294044
6	35	4.94941572877158
6	36	4.536189391419853
6	37	4.887342500053403
6	38	5.333042429836215
6	39	5.032478739271642
6	40	3.073483863181451
6	41	2.2580070181415017
6	42	7.48886797674599
6	43	12.210782139724898
6	44	10.641025393333557
6	45	8.795845465816054
6	46	10.409717584379106
6	47	10.770499242609404
6	48	10.34795557831264
6	49	12.720053428786468
6	50	10.936978542702178
6	51	10.538813400774345
6	52	10.884368134295134
6	53	10.500565534059007
6	54	11.845839724554164
6	55	10.583470873212878
6	56	10.794130166786644
7	1	3.0331860358678853
7	2	3.8325043292000416
7	3	4.216366430059061
7	4	3.5521473497257503
7	5	4.76767700228983
7	6	3.6627175168853654
7	7	4.174577702699743
7	8	4.138058548177391
7	9	3.895724001909265
7	10	4.724668550313218
7	11	5.729163651155876
7	12	6.392386946494355
7	13	7.562721729651212
7	14	8.43752358318676
7	15	7.644547097763329
7	16	7.393110413326422
7	17	7.431301457563183
7	18	6.1970760454440885
7	19	5.774035700903138
7	20	5.8686060422239485
7	21	4.005367641263511
7	22	4.985217438408128
7	23	3.602989577575322
7	24	5.243436181282526
7	25	4.491626121474042
7	26	3.96702529084358
7	27	4.158081797581424
7	28	3.920202493033102
7	29	4.227924566678254
7	30	3.814880158650517
7	31	3.955656926302776
7	32	5.623833544272518
7	33	4.279266222339999
7	34	4.66461047419601
7	35	4.814709846418641
7	36	2.2201109600473004
7	37	2.7049895588769353
7	38	2.7671600768950384
7	39	5.02463560201674
7	40	-3.8998494701518815
7	41	-7.817875334799675
7	42	5.55337778066756
7	43	9.03176253188176
7	44	8.080829314902182
7	45	6.39074742660037
7	46	9.181482290261457
7	47	10.172852183785874
7	48	10.198543813606754
7	49	11.674955389570783
7	50	10.411096189761
7	51	10.86449967528415
7	52	11.349662251942188
7	53	11.33017337719626
7	54	12.008388744162001
7	55	11.030137539879544
7	56	10.175110558943505
8	1	2.358816287968729
8	2	3.6455295392840696
8	3	4.427710967874191
8	4	3.4903826438433967
8	5	4.068937506491515
8	6	4.029104071507213
8	7	3.8481071144644474
8	8	3.783016531370663
8	9	3.9852198002285917
8	10	3.766685357035904
8	11	5.872440962080244
8	12	7.302470980107799
8	13	6.8530578641049935
8	14	8.134162238648948
8	15	7.605471467511229
8	16	6.875883522570124
8	17	6.522057760084191
8	18	6.484050835360055
8	19	4.748405448802298
8	20	5.34213545398865
8	21	3.49948528832234
8	22	3.8898392871476233
8	23	3.4101324347181787
8	24	4.050999206492612
8	25	3.5521303231547137
8	26	3.548117727818365
8	27	3.4475775959007535
8	28	3.583647871184361
8	29	3.8287649028127078
8	30	3.279586041003462
8	31	3.0762451615968907
8	32	4.787699090491007
8	33	1.4259048778021821
8	34	2.488139885960713
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115	44	2.3043587266668872
115	45	2.7025121324827204
115	46	-6.155282415620896
115	47	-7.734500757390597
115	48	-6.722044421687361
115	49	-9.688279904546865
115	50	-4.6580214572978225
115	51	-1.067853265892321
115	52	-1.0356318657048682
115	53	-1.472767799274326
115	54	-0.8724936087791733
115	55	-0.24486246012045498
115	56	2.2824635001199773
116	1	1.6144553857130859
116	2	2.630491945299113
116	3	3.245004200956892
116	4	2.1535405385802378
116	5	3.4170578072433955
116	6	2.4072995602290135
116	7	2.695475535517076
116	8	2.895798486257881
116	9	2.528076943085736
116	10	2.826835732975752
116	11	-0.8877094138596036
116	12	-3.228356087561373
116	13	-2.854460932887484
116	14	-10.916965580899927
116	15	-4.6178368031654635
116	16	-9.157199184196795
116	17	-12.602754269990992
116	18	-4.78512209697077
116	19	-4.544075754205224
116	20	-4.715759282853455
116	21	1.8521168672697073
116	22	1.3048768811325857
116	23	1.6424632617858492
116	24	2.2186683794249404
116	25	1.372431075034413
116	26	3.0421026902243824
116	27	2.290434738757895
116	28	4.088159149379848
116	29	1.7310205419104534
116	30	2.4216913041613566
116	31	1.8867714773863646
116	32	-2.7724512854488452
116	33	2.4642507424638396
116	34	1.9355083070133432
116	35	2.17152099192948
116	36	3.6179437773847667
116	37	3.745237236895509
116	38	3.8677792719414796
116	39	5.886864704183925
116	40	9.4685715824797
116	41	6.295901754983607
116	42	2.625359204816167
116	43	-8.835884526941769
116	44	2.28593767403531
116	45	0.8551437114300917
116	46	-5.914492941936684
116	47	-8.498974441601122
116	48	-7.2194128427399935
116	49	-5.202753588757391
116	50	-4.646179352034665
116	51	-1.2678532658923203
116	52	-1.898789760441712
116	53	-3.2464520098006417
116	54	-3.552756766673909
116	55	-3.233020354857299
116	56	-2.1135891314589728
117	1	0.5552448593972983
117	2	1.9967200154745512
117	3	2.0300919202551384
117	4	1.848715977176731
117	5	2.35346131601532
117	6	2.5279135953167327
117	7	1.623107114464446
117	8	1.9615879599420936
117	9	1.6114102764190719
117	10	1.15359011894067
117	11	-11.131130466491182
117	12	-12.296338543701726
117	13	-9.108846897799765
117	14	-14.43012347563677
117	15	-9.431433294393532
117	16	-11.793164096477497
117	17	-9.725561287534852
117	18	-3.2588063074970854
117	19	-3.8730231226262752
117	20	-4.1828645460113485
117	21	-1.8255147116776627
117	22	-1.103017855709517
117	23	-0.1470104224246782
117	24	-4.866857936364532
117	25	-0.9323934863690972
117	26	-0.11140608170543942
117	27	-0.04289859457544054
117	28	1.515790728327218
117	29	0.42400299805080266
117	30	0.6629193743367949
117	31	-0.6987548384031079
117	32	-13.015872338080424
117	33	-2.005047503150198
117	34	-1.8868601140392869
117	35	-1.6705842712284174
117	36	0.3745227247531844
117	37	1.0456758333867384
117	38	0.014709096502883767
117	39	2.215812072604976
117	40	5.683483863181451
117	41	3.3046736848081686
117	42	0.7788679767459925
117	43	-7.5858845269417685
117	44	-0.012307939999779194
117	45	0.635845465816054
117	46	-4.605282415620895
117	47	-8.376167424057263
117	48	-4.480377755020694
117	49	-5.579946571213531
117	50	-3.633021457297822
117	51	-2.2678532658923203
117	52	-1.7189651990382018
117	53	-4.314434465940995
117	54	-5.5724936087791725
117	55	-5.05319579345379
117	56	-0.5675364998800241
118	1	1.8356502648027018
118	2	2.450548844303377
118	3	2.8431549833182004
118	4	2.1741664276271777
118	5	2.906389243943252
118	6	2.580841523244665
118	7	2.3967557631130916
118	8	2.630506878861013
118	9	2.3118607268695186
118	10	2.019581109931657
118	11	-7.134508844869561
118	12	-7.376293498656679
118	13	-9.72145951041238
118	14	-0.5044477999610919
118	15	-15.80418104214128
118	16	-4.628749682063084
118	17	-6.648984710958278
118	18	-0.8534009020916784
118	19	-1.6365366361397875
118	20	-2.111918600065401
118	21	1.9211069099439584
118	22	2.214549711858048
118	23	2.0016382262239674
118	24	-2.6405065850131813
118	25	2.8795434505678443
118	26	3.602557882258523
118	27	3.749894198217355
118	28	3.9380880256245163
118	29	4.270849844897654
118	30	4.241748203165621
118	31	2.6492181345698675
118	32	-2.519250716458803
118	33	3.0602678121651152
118	34	2.5286804265012535
118	35	3.234821134176986
118	36	5.9927659679964265
118	37	6.5738289865398905
118	38	7.32439378118757
118	39	9.100947207740113
118	40	12.478528908226497
118	41	10.435304315438799
118	42	4.781120228998244
118	43	4.2857370946798525
118	44	3.1408452131533764
118	45	2.6538634838340727
118	46	-3.500552685891165
118	47	-4.087879135768976
118	48	-4.015287664930604
118	49	-6.197063688330649
118	50	-1.5722106464870116
118	51	-1.3894748875139413
118	52	-1.2842805143535188
118	53	-1.2727677992743267
118	54	-6.555601716887281
118	55	-3.172565162823158
118	56	-2.435779743123266
119	1	1.3999418290942671
119	2	2.136871530626067
119	3	2.477061617224841
119	4	2.329776583237333
119	5	3.0693704069244134
119	6	1.8953378377409749
119	7	2.6268949932523213
119	8	2.3479515963057267
119	9	2.3765617915705874
119	10	1.6649537553043032
119	11	-12.240978951339667
119	12	-11.754671877035058
119	13	-3.3209681099209796
119	14	-2.5134568089701013
119	15	-7.2685545065147465
119	16	-8.740133793447196
119	17	-9.225561287534852
119	18	-1.4103214590122377
119	19	-1.3654473650505174
119	20	-1.1487736369204384
119	21	1.451000439837486
119	22	0.04092153822987399
119	23	-0.13185890727316263
119	24	-5.779736724243321
119	25	1.3668489378733284
119	26	2.7901090698097093
119	27	2.017707466030622
119	28	2.277154364690851
119	29	3.1209726950205052
119	30	3.049283010700428
119	31	-0.38436089900917025
119	32	-4.716629913837998
119	33	0.7146494665467742
119	34	-1.042163144342318
119	35	1.1324460318018872
119	36	3.44649242172288
119	37	3.5418879545988595
119	38	4.63970909650288
119	39	4.208236315029218
119	40	7.160756590454181
119	41	2.7440676242021063
119	42	-0.8347683868903708
119	43	-1.4570966481538896
119	44	0.3513284236365841
119	45	-3.9096090796384892
119	46	-7.593918779257258
119	47	-11.103440151329991
119	48	-9.226589876232817
119	49	-7.655704146971107
119	50	-8.049688123964488
119	51	-9.070883568922623
119	52	-9.69623792631093
119	53	-10.57579810230463
119	54	-10.992948154233718
119	55	-13.106226096484093
119	56	-10.70768801503154
120	1	1.9453963745488139
120	2	2.95505334880788
120	3	3.658879799043021
120	4	2.4812917347524888
120	5	3.79664313419714
120	6	2.9862469286500684
120	7	2.566288932646266
120	8	3.014618262972398
120	9	2.922016337025127
120	10	1.8770749674255143
120	11	-12.51370622406694
120	12	-2.2698233921865736
120	13	-5.7149075038603705
120	14	-3.816487112000406
120	15	-6.63219087015111
120	16	-11.01286106617447
120	17	-8.528591590565156
120	18	-3.955776004466781
120	19	-1.4260534256565798
120	20	-3.542713030859833
120	21	0.632818621655673
120	22	-0.019684522376181235
120	23	-0.9803437557580104
120	24	-7.507009451516048
120	25	-0.8452722742478826
120	26	2.4567757364763736
120	27	2.048010496333653
120	28	2.428669516206007
120	29	2.2118817859295916
120	30	0.9583739197913346
120	31	-1.1116336262818969
120	32	-8.049963247171334
120	33	-2.012623260725956
120	34	-7.5270116291908025
120	35	-2.4433115439556907
120	36	-0.7353257600953
120	37	-1.7005362878253827
120	38	-0.7239272671334795
120	39	0.32944843624133924
120	40	-4.536213106515518
120	41	-2.740780860646378
120	42	-5.834768386890371
120	43	-4.305581496638737
120	44	-6.5880655157573536
120	45	-11.273245443274854
120	46	-11.109070294408774
120	47	-13.648894696784536
120	48	-9.52962017926312
120	49	-13.776916268183228
120	50	-9.07999115426752
120	51	-9.616338114377168
120	52	-9.332601562674567
120	53	-11.151555678062206
120	54	-10.841433002718567
120	55	-10.560771551029548
120	56	-8.101627408970932
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1577.0
5	204.0
6	14.0
7	61.0
8	154.0
9	108.0
10	83.0
11	103.0
12	133.0
13	132.0
14	192.0
15	239.0
16	324.0
17	453.0
18	488.0
19	584.0
20	759.0
21	945.0
22	1097.0
23	1212.0
24	1366.0
25	1406.0
26	1488.0
27	1501.0
28	1675.0
29	1770.0
30	2067.0
31	2391.0
32	2924.0
33	3622.0
34	4226.0
35	4780.0
36	5054.0
37	4163.0
38	2084.0
39	482.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	98.94105613605825	0.8543751629530093	0.15041816249172701	0.05415053849702172
8	99.50462285152724	0.3108642024829025	0.06818956699624958	0.1163233789936022
9	98.87665242422419	0.5496379210045937	0.4011955627770757	0.17251409199414253
10	56.085918854415276	23.571528850203567	10.960470106897175	9.382082188483984
11	36.74815988447885	28.82814223541445	18.445277872485512	15.978420007621185
12	30.43059705982632	13.006157116784662	21.227011090832516	35.33623473255651
13	24.317602936162533	12.131726198832755	23.08016285273059	40.470508012274124
14	19.719553050211637	15.24002487512287	31.312563943108184	33.727858131557305
15	28.398063129131422	17.253018826224107	24.71117719153724	29.637740853107232
16	33.43231476093979	18.03936518127646	22.682129170763027	25.846190887020725
17	27.733755942947703	19.440710947060122	29.075808943007885	23.749724166984294
18	27.61096736666867	20.560803899151573	28.700082235192653	23.128146498987103
19	33.85217129676061	22.60555611272691	19.135492929495538	24.40677966101695
20	27.37868362454613	29.440911552889727	19.8960861802644	23.284318642299745
21	25.715546462883847	22.15335860561205	28.246785807409193	23.88430912409491
22	24.72072361164036	18.986783257455727	26.134654339062596	30.157838791841318
23	24.66256192214356	21.856761797797876	23.647740719199376	29.832935560859188
24	24.41587613565713	22.937766992238423	27.76919837147269	24.877158500631758
25	30.525471319695146	24.089450461291616	21.13517849979944	24.2498997192138
26	23.288331726133077	22.23561555769849	24.379218900675024	30.09683381549341
27	23.864639332423977	19.2991254112172	23.351119313166972	33.48511594319184
28	22.870513567253813	26.017795095302176	25.20034545783205	25.911345879611964
29	31.130145229880448	22.67511835031694	22.89376554601621	23.300970873786408
30	25.777394374673996	20.296513260843398	32.000561730128794	21.92553063435381
31	25.69028576021864	24.958803906595396	27.418512117680155	21.932398215505806
32	32.83186905764833	18.494002487262804	24.58378465118145	24.09034380390741
33	27.637474132561827	19.78180941474293	26.000040182428226	26.580676270267013
34	23.570740324393768	25.30110807772603	30.261361811466198	20.866789786414003
35	24.555067316759967	22.733200906920285	33.89112943678646	18.820602339533295
36	25.47901184976903	26.99939746937136	27.10986141795541	20.411729262904196
37	25.62520102926986	22.34641363782567	30.845931167578	21.182454165326472
38	30.766141178833216	24.155035646149212	25.54674164072698	19.53208153429059
39	22.729743331655765	18.570709612481124	22.778057372924007	35.921489682939104
40	15.451648041311977	14.904990519223787	22.17291322063985	47.47044821882439
41	17.699670167344543	16.467350614136265	24.632226471599992	41.2007527469192
42	25.839512871525894	21.57512911718012	28.134482827917445	24.45087518337654
43	13.482942537989334	12.307537486162826	25.132333702324644	49.077186273523196
44	15.580645161290322	14.10483870967742	20.239919354838708	50.074596774193544
45	22.62870452172516	21.89181591840013	31.664123363585254	23.815356196289457
46	20.544619027552024	23.145305319768024	28.85236690546425	27.457708747215698
47	19.632037237671042	23.289595120581037	28.817061915653465	28.26130572609446
48	22.434444154645586	26.122023152699477	29.996188030415503	21.44734466223943
49	15.900491103775863	17.1524031881491	20.531760727799693	46.41534498027534
50	26.108889201519386	21.731615652069056	28.504532025644636	23.65496312076693
51	22.64989939637827	27.07645875251509	29.728370221327964	20.54527162977867
52	21.502757537941307	22.456825409605088	32.569139728674365	23.471277323779237
53	21.660236315408728	20.983039948557188	34.43252150148702	22.92420223454706
54	21.15357580382785	22.10953346855984	26.126162311971562	30.610728415640754
55	21.89025737879488	26.043278214221132	29.788431014044324	22.278033392939665
56	25.953830389970467	20.19568842545155	28.905229743033374	24.945251441544613
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	1.0
10	0.5
11	0.0
12	1.0
13	9.5
14	17.0
15	25.5
16	34.0
17	61.0
18	88.0
19	104.5
20	121.0
21	135.0
22	160.0
23	171.0
24	197.5
25	224.0
26	263.5
27	303.0
28	391.5
29	557.0
30	634.0
31	684.0
32	734.0
33	803.0
34	872.0
35	899.5
36	927.0
37	835.5
38	772.5
39	801.0
40	764.5
41	728.0
42	758.5
43	789.0
44	868.0
45	947.0
46	1011.5
47	1411.0
48	1746.0
49	2170.5
50	2595.0
51	2497.5
52	2400.0
53	2317.5
54	2252.0
55	2269.0
56	2368.5
57	2468.0
58	2488.0
59	2508.0
60	2764.0
61	3020.0
62	3312.5
63	3672.5
64	3740.0
65	3446.0
66	3152.0
67	2899.0
68	2646.0
69	2379.0
70	2112.0
71	1910.5
72	1520.0
73	1331.0
74	1198.0
75	1065.0
76	845.0
77	625.0
78	494.5
79	295.5
80	227.0
81	178.0
82	129.0
83	88.0
84	47.0
85	35.0
86	23.0
87	13.0
88	1.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.020055754998896932
10	0.0
11	0.0
12	0.0
13	0.0
14	0.02406690599867632
15	0.1805017949900724
16	0.040111509997793865
17	0.02406690599867632
18	0.008022301999558773
19	0.01203345299933816
20	0.02406690599867632
21	0.008022301999558773
22	0.0
23	0.0
24	0.0
25	0.0020055754998896933
26	0.17047391749062393
27	0.01805017949900724
28	0.14440143599205793
29	0.01805017949900724
30	0.030083632498345402
31	0.19855197448907963
32	0.014039028499227854
33	0.17649064399029302
34	0.0902508974950362
35	0.044122660997573254
36	0.14239586049216824
37	0.23465233348709413
38	0.13236798299271976
39	0.373037042979483
40	0.5756001684683419
41	0.8864643709512444
42	0.20055754998896932
43	0.35298128798058603
44	0.5234552054712099
45	0.11431780349371251
46	0.056156113996911415
47	0.03810593449790418
48	0.03610035899801448
49	0.3549868634804757
50	0.2085798519885281
51	0.32289765548224064
52	0.3589980144802551
53	0.19454082348930027
54	0.13637913399249915
55	0.1805017949900724
56	0.17649064399029302
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	49861.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.19405948536932
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.21581705574083	78.48218046168348
2	4.321105288232491	7.276227913599807
3	1.1576941400666985	2.924129078839173
4	0.4121010004764173	1.387858245923668
5	0.24773701762744166	1.0428992599426405
6	0.11910433539780849	0.6016726499669081
7	0.10004764173415913	0.5896391969675698
8	0.0857551214864221	0.5776057439682317
9	0.050023820867079564	0.37905376947915204
>10	0.26202953787517863	3.6922644952969255
>50	0.014292520247737018	0.7681354164577525
>100	0.014292520247737018	2.2783337678746918
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACGAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG	255	0.5114217524718718	No Hit
ACGAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG	251	0.503399450472313	No Hit
ACGAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG	180	0.3610035899801448	No Hit
ACGAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	175	0.35097571248069637	No Hit
ACGAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG	171	0.34295341048113753	No Hit
ACGAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAACGATTAAG	104	0.2085798519885281	No Hit
ACGAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC	79	0.15844046449128577	No Hit
ACGAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGCCTCAAAGATTAAG	71	0.14239586049216824	No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC	67	0.13437355849260946	No Hit
ACGAGCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGCGCTAATACATGCCGAC	59	0.11832895449349191	No Hit
ACGAGCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACCACCCATTCGAACGTC	54	0.10830107699404344	No Hit
ACGAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	53	0.10629550149415376	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0020055754998896933	0.0	0.0	0.0	0.0
8	0.0020055754998896933	0.0	0.0	0.0	0.0
9	0.0020055754998896933	0.0	0.0	0.0	0.0
10	0.004011150999779387	0.0	0.0	0.0	0.0
11	0.004011150999779387	0.0	0.0	0.0	0.0
12	0.0060167264996690804	0.0	0.0	0.0	0.0
13	0.0060167264996690804	0.0	0.0	0.0	0.0
14	0.008022301999558773	0.0	0.0	0.0	0.0
15	0.010027877499448466	0.0	0.0	0.0	0.0
16	0.010027877499448466	0.0	0.0	0.0	0.0
17	0.012033452999338161	0.0	0.0	0.0	0.0
18	0.01805017949900724	0.0	0.0	0.0	0.0
19	0.01805017949900724	0.0	0.0	0.0	0.0
20	0.01805017949900724	0.0	0.0	0.0	0.0
21	0.01805017949900724	0.0	0.0	0.0	0.0
22	0.01805017949900724	0.0	0.0	0.0	0.0
23	0.01805017949900724	0.0	0.0	0.0	0.0
24	0.01805017949900724	0.0	0.0	0.0	0.0
25	0.020055754998896932	0.0	0.0	0.0	0.0
26	0.022061330498786627	0.0	0.0	0.0	0.0
27	0.026072481498566013	0.0	0.0	0.0	0.0
28	0.03208920799823509	0.0	0.0	0.0	0.0
29	0.03208920799823509	0.0	0.0	0.0	0.0
30	0.03409478349812479	0.0	0.0	0.0	0.0
31	0.03610035899801448	0.0	0.0	0.0	0.0
32	0.03610035899801448	0.0	0.0	0.0	0.0
33	0.03610035899801448	0.0	0.0	0.0	0.0
34	0.03610035899801448	0.0	0.0	0.0	0.0
35	0.03610035899801448	0.0	0.0	0.0	0.0
36	0.03610035899801448	0.0	0.0	0.0	0.0
37	0.03610035899801448	0.0	0.0	0.0	0.0
38	0.03610035899801448	0.0	0.0	0.0	0.0
39	0.03610035899801448	0.0	0.0	0.0	0.0
40	0.03610035899801448	0.0	0.0	0.0	0.0
41	0.040111509997793865	0.0	0.0	0.0	0.0
42	0.040111509997793865	0.0	0.0	0.0	0.0
43	0.040111509997793865	0.0	0.0	0.0	0.0
44	0.040111509997793865	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCCGTC	20	3.8599322E-4	50.72569	38
AATTCCC	15	0.008048076	50.51886	35
CTCGCCT	15	0.008048076	50.51886	41
AACGATT	50	5.456968E-12	50.211754	47
CCTGCCG	15	0.008244701	50.211754	48
GCGTGTT	15	0.008244701	50.211754	48
AAGATTC	20	4.059254E-4	50.211754	48
TATCTCA	15	0.008244701	50.211754	48
TTTGGTA	15	0.008244701	50.211754	46
TTGGTAT	15	0.008244701	50.211754	47
ACGATTC	25	2.0074782E-5	50.211754	48
TACCAAG	20	4.059254E-4	50.211754	47
CGATTCA	25	2.0074782E-5	50.211754	49
GTTTGGT	15	0.008277816	50.16093	45
AATTATC	15	0.008277816	50.16093	45
TCAACGA	50	7.2759576E-12	50.16093	45
AATCACT	15	0.0083110295	50.110214	50
CAAACAA	15	0.0083110295	50.110214	50
ATGATTT	25	2.0439331E-5	50.059597	33
GTGTGGC	20	4.120618E-4	50.059597	33
>>END_MODULE