FastQCFastQC Report
Fri 17 Jun 2016
SRR1526320_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526320_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65464
Sequences flagged as poor quality0
Sequence length56
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATTGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC1060.1619210558474887No Hit
CGTATTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC970.14817304167175852No Hit
CGTATTGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT830.12678724184284493No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACCT150.0078833550.80171639
ATCCTAC150.00798121950.64357837
AGCCCTT251.9148498E-550.64357837
CGGCCAA309.452069E-750.64357837
AGACAAC150.00798121950.64357837
AGAGATC150.00798121950.64357837
GTACGCC150.00798121950.64357837
AAACTCA150.00810482350.44728536
CCGGCCA354.834328E-850.44728536
GACTTCA150.00817966550.3302434
CCAACAC750.050.21373743
CTAATCG150.00828025350.1750249
TTTACAA150.00830554350.1363645
CAACGAT252.0325622E-550.1363646
AACTTCA150.00830554350.1363647
TTATATT150.00830554350.1363645
ACGATTA252.0325622E-550.1363648
TATCTCA150.00830554350.1363648
AACGATT252.0325622E-550.1363647
CATTTGA150.00830554350.1363646