SRR1526319_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1526319_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	44818
Sequences flagged as poor quality	0
Sequence length	56
%GC	53

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAACGCGGGGATGAGATGGACTCAAATGAATTGACGGGTCCGCACAGTTCTGAGTC	538	1.2004105493328574	No Hit
GAACGCGGGGATGAGATGGACTTAAAGGAATTGACGGGTCCGCACAGTTCTGAGTC	529	1.1803293319648356	No Hit
GAACGCGGGGATGAGATGGACTCAAAGGAATTGACGGGTCCGCACAGTTCTGAGTC	519	1.1580168682225893	No Hit
GAACGCGGGGATGAGATGGACTTAAATGAATTGACGGGTCCGCACAGTTCTGAGTC	464	1.035298317640234	No Hit
GAACGCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCCCCGCGGCCAAGGAGA	299	0.667142665893168	No Hit
GAACGCGGGGATGAGATGGACTCAAATGAATTGACGGGTACGCACAGTTCTGAGTC	219	0.4886429559551966	No Hit
GAACGCGGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTC	199	0.44401802847070376	No Hit
GAACGCGGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTC	195	0.4350930429738052	No Hit
GAACGCGGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTC	195	0.4350930429738052	No Hit
GAACGCGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCCCCGCGGCCAAGGAGAG	164	0.36592440537284127	No Hit
GAACGCGGGGATGCGATGGACTTAAAGGAATTGACGGGTACGCCCAGTCCTGAGTC	134	0.298987014146102	No Hit
GAACGCGGGGATGCGATGGACTCAAAGGAATTGACGGGTACGCCCAGTCCTGAGTC	133	0.2967557677718774	No Hit
GAACGCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA	122	0.2722120576554063	No Hit
GAACGCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCCCGGCCAAGGAGA	117	0.2610558257842831	No Hit
GAACGCGGGGATGCGATGGACTCAAATGAATTGACGGGTACGCCCAGTCCTGAGTC	112	0.24989959391315988	No Hit
GAACGCGGGGATGCGATGGACTTAAATGAATTGACGGGTACGCCCAGTCCTGAGTC	110	0.2454371011647106	No Hit
GAACGCAGGGATGAGATGGACTTAAAGGAATTGACGGGTCCGCACAGTTCTGAGTC	83	0.18519344906064528	No Hit
GAACGCGGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCCCAGTTCTGAGTC	78	0.1740372171895221	No Hit
GAACGCAGGGATGAGATGGACTCAAAGGAATTGACGGGTCCGCACAGTTCTGAGTC	74	0.16511223169262348	No Hit
GAACGCGGGGATGAGATGGACTCAAATGAATTGACGGGTACGCCCAGTTCTGAGTC	74	0.16511223169262348	No Hit
GAACGCGGGGATGAGATGGACTCAAAGGAATTGACGGGTCAGCACAGTTCTGAGTC	74	0.16511223169262348	No Hit
GAACGCGGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTC	70	0.15618724619572494	No Hit
GAACGCGGGGATGAGATGGACTTAAAGGAATTGACGGGTCAGCACAGTTCTGAGTC	66	0.14726226069882636	No Hit
GAACGCAGGGATGAGATGGACTTAAATGAATTGACGGGTCCGCACAGTTCTGAGTC	66	0.14726226069882636	No Hit
GAACGCAGGGATGAGATGGACTCAAATGAATTGACGGGTCCGCACAGTTCTGAGTC	59	0.1316435360792539	No Hit
GAACGCGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAG	54	0.12048730420813067	No Hit
GAACGCGGGGATGAGATGGACTTAAAGGAATTGACGGGTCTGCACAGTTCTGAGTC	52	0.11602481145968138	No Hit
GAACGCGGGGATGAGATGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTC	52	0.11602481145968138	No Hit
GAACGCGGGGATGCGATGGACTCAAAGGAATTGACGGGTACGCCCAGTTCTGAGTC	52	0.11602481145968138	No Hit
GAACGCGGGGATGAGATGGACTCAAATGAATTGACGGGTCTGCACAGTTCTGAGTC	50	0.1115623187112321	No Hit
GAACGCGGGGATGCGATGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTC	50	0.1115623187112321	No Hit
GAACGCGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCCAGGAGAG	49	0.10933107233700745	No Hit
GAACGCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGACCCGCGGCCAAGGAGA	48	0.10709982596278281	No Hit
GAACGCGGGGATGAGATGGACTTAAATGAATTGACGGGTCAGCACAGTTCTGAGTC	48	0.10709982596278281	No Hit
GAACGCGGGGATGAGATGGACTCAAAGGAATTGACGGGTCTGCACAGTTCTGAGTC	47	0.10486857958855818	No Hit
GAACGCGGGGATGAGATGGACTCAAATGAATTGACGGGTCAGCACAGTTCTGAGTC	46	0.10263733321433353	No Hit
GAACGCGGGGATGAGATGGACTTAAATGAATTGACGGGTCTGCACAGTTCTGAGTC	45	0.10040608684010889	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGACCC	15	0.008031929	50.5362	39
ACCAACC	15	0.008031929	50.5362	38
TCAGCAC	50	5.456968E-12	50.5362	39
GCACGCC	15	0.008031929	50.5362	38
CTGCACA	25	1.9291128E-5	50.5362	40
CTACTGT	15	0.008031929	50.5362	40
GTACGAC	25	1.9291128E-5	50.5362	38
CAGCACA	55	0.0	50.536198	40
GTCAGCA	55	0.0	50.536198	38
TACTGTC	15	0.008067964	50.4791	41
CGCCCAC	55	0.0	50.479095	41
CGTACAG	20	3.9497978E-4	50.479095	41
ATCCTAC	20	3.9941378E-4	50.365276	37
CCGGCGA	20	3.9941378E-4	50.365276	36
CGGCCCA	30	1.005692E-6	50.13917	44
ACGGTCC	15	0.008286704	50.13917	34
GCCCACT	60	0.0	50.082962	42
AGTAGTG	15	0.008323586	50.082962	43
CCAAAAC	35	5.070615E-8	50.082962	43
CAGTAGT	15	0.008323586	50.082962	42

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content