FastQCFastQC Report
Fri 17 Jun 2016
SRR1526318_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526318_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36617
Sequences flagged as poor quality0
Sequence length56
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC1570.4287625966081328No Hit
TCAAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1360.3714121855968539No Hit
TCAAAGGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC850.2321326159980337No Hit
TCAAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTCCCTACCAAGCGC670.18297512084550893No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC630.17205123303383674No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGACCACCCGACTGCTCTTC600.16385831717508262No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC590.16112734522216457No Hit
TCAAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG520.14201054155173828No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC470.12835568178714807No Hit
TCAAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG400.10923887811672174No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACAA150.00796700350.62083439
TTCGTTT203.8857397E-450.62083438
ACCCGAT150.00796700350.62083441
CAACAAA203.8857397E-450.62083440
TACGCCC203.8857397E-450.62083438
CCCTGGA150.00796700350.62083441
GCTCCCC150.00796700350.62083440
GCCCTTG150.00796700350.62083438
GTACGCC203.9394418E-450.48061437
CCAACAC251.9505092E-550.4107943
TGCCTAG354.782305E-850.41078643
GCAGCAT203.993732E-450.3411642
CCCGATT150.00814376350.3411642
TTGCCTA354.8350557E-850.3411642
TCCCCCA203.993732E-450.3411642
CGACTCC354.8881702E-850.2717344
GTCGCGG150.00818840550.27172536
TATGCTT150.00818840550.27172535
CAACACA309.823852E-750.27172544
ATGCTTG150.00818840550.27172536