##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526318_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 36617 Sequences flagged as poor quality 0 Sequence length 56 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.36354698637245 39.0 38.0 39.0 33.0 40.0 2 36.57413223366196 39.0 37.0 39.0 30.0 40.0 3 35.62607532020646 39.0 36.0 39.0 26.0 40.0 4 34.73258322637027 39.0 35.0 39.0 18.0 40.0 5 34.89537646448371 39.0 35.0 39.0 20.0 40.0 6 36.21214190130267 38.0 35.0 39.0 29.0 40.0 7 36.56279870005735 38.0 36.0 39.0 31.0 40.0 8 36.44271786328754 38.0 36.0 39.0 31.0 40.0 9 36.485976459021764 38.0 36.0 39.0 31.0 40.0 10 36.14105470136822 38.0 36.0 39.0 29.0 40.0 11 34.738372886910454 38.0 32.0 39.0 27.0 40.0 12 32.94010978507251 36.0 31.0 39.0 19.0 40.0 13 32.55651746456564 36.0 30.0 39.0 17.0 40.0 14 32.02072807712265 36.0 29.0 39.0 15.0 40.0 15 32.66600213015813 37.0 30.0 39.0 17.0 40.0 16 32.49081027937843 36.0 30.0 38.0 18.0 40.0 17 32.49356856105088 36.0 30.0 38.0 19.0 40.0 18 32.58879755304913 36.0 30.0 38.0 19.0 40.0 19 33.56847912171942 36.0 31.0 38.0 24.0 40.0 20 33.73788131195893 36.0 31.0 38.0 24.0 40.0 21 35.20583335609143 38.0 35.0 39.0 27.0 40.0 22 34.90985061583417 38.0 35.0 39.0 26.0 40.0 23 35.19439058360871 38.0 35.0 39.0 27.0 40.0 24 34.713848758773246 38.0 33.0 39.0 26.0 40.0 25 35.05314471420378 38.0 34.0 39.0 27.0 40.0 26 35.4055220252888 38.0 35.0 39.0 28.0 40.0 27 35.38940929076659 38.0 35.0 39.0 28.0 40.0 28 35.326678865008056 38.0 35.0 39.0 29.0 40.0 29 35.14318485949149 38.0 35.0 39.0 28.0 40.0 30 35.01431029303329 38.0 35.0 39.0 27.0 40.0 31 34.696315918835516 38.0 35.0 39.0 26.0 40.0 32 33.761149192997784 38.0 33.0 39.0 20.0 40.0 33 35.03096922194609 38.0 35.0 39.0 28.0 40.0 34 34.64814157358604 38.0 35.0 39.0 27.0 40.0 35 34.67523281535899 38.0 35.0 39.0 27.0 40.0 36 32.49788349673649 35.0 31.0 38.0 23.0 39.0 37 32.223666602943986 35.0 31.0 38.0 22.0 39.0 38 31.313024005243467 35.0 30.0 38.0 15.0 39.0 39 29.403282628287407 34.0 27.0 38.0 5.0 39.0 40 25.03804243930415 30.0 11.0 37.0 4.0 39.0 41 21.704263047218504 25.0 9.0 33.0 4.0 37.0 42 25.49212114591583 30.0 20.0 34.0 4.0 37.0 43 21.595898080126716 26.0 8.0 33.0 4.0 36.0 44 22.627304257585276 27.0 9.0 33.0 4.0 37.0 45 25.16833711117787 30.0 19.0 34.0 4.0 37.0 46 25.176775814512386 30.0 14.0 36.0 4.0 38.0 47 24.41816642543081 30.0 9.0 36.0 4.0 38.0 48 24.442936341043776 30.0 10.0 35.0 4.0 38.0 49 21.739820302045498 26.0 5.0 35.0 4.0 38.0 50 24.195046016877406 29.0 4.0 35.0 4.0 38.0 51 25.699893492093835 32.0 4.0 38.0 4.0 39.0 52 25.611382691099763 32.0 4.0 38.0 4.0 39.0 53 25.194882158560233 32.0 4.0 38.0 4.0 39.0 54 24.248627686593657 31.0 4.0 38.0 4.0 39.0 55 24.505721386241362 31.0 4.0 38.0 4.0 39.0 56 23.394024633367014 30.0 4.0 37.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.3104028411961224 1 2 2.45823262075681 1 3 3.45494321403784 1 4 4.372866589190757 1 5 4.045885476369243 1 6 2.71772495649423 1 7 2.551546559972955 1 8 2.6629503037821465 1 9 2.694307247614887 1 10 2.2509628476038017 1 11 -3.2533564670579693 1 12 -7.637104988043475 1 13 -22.008343650450847 1 14 -18.984122777036056 1 15 -13.833599639913693 1 16 -21.596854396972624 1 17 -22.244550826170038 1 18 -21.748341408316165 1 19 -6.093015204249667 1 20 -11.693001970297296 1 21 -9.927493492914483 1 22 -4.051390582588329 1 23 -4.255134195114742 1 24 -4.52375358705952 1 25 -5.20943242600266 1 26 1.4373863796297641 1 27 1.398928393135833 1 28 1.0828242204536593 1 29 1.0337796248009923 1 30 1.2603579934252593 1 31 0.7346841969744062 1 32 -14.020834440173026 1 33 0.545808717961485 1 34 -0.1814486249646592 1 35 -1.1542186118822215 1 36 0.9044901775168555 1 37 2.0840163956420703 1 38 2.0977001636451185 1 39 4.4370418135617165 1 40 7.785666944335592 1 41 -1.7995338295708159 1 42 -5.529379751809184 1 43 -12.982697037310398 1 44 -15.608793765491857 1 45 -15.757916227829176 1 46 -18.48511251794099 1 47 -17.85390401700841 1 48 -17.414016837476215 1 49 -15.055544006739117 1 50 -17.567796749120372 1 51 -18.81760849052033 1 52 -18.70450468462669 1 53 -18.26141771736542 1 54 -18.19852097103181 1 55 -18.17562311381102 1 56 -17.292702720470242 2 1 0.8041808402006012 2 2 2.062015597362084 2 3 2.9074071264320693 2 4 4.118511188493898 2 5 3.774606232964558 2 6 2.0034392422085148 2 7 1.788978117962003 2 8 2.1164097363356618 2 9 1.9929632953998535 2 10 2.1220429869766235 2 11 -2.4156262530211343 2 12 -10.491759044788125 2 13 -12.755481529992906 2 14 -14.44305757046562 2 15 -9.249727066494081 2 16 -3.865147079899451 2 17 -2.2375821850550537 2 18 -10.089307062870667 2 19 -2.610685687076945 2 20 -3.420229446653689 2 21 1.253193415995419 2 22 1.7146621799801096 2 23 0.8712968651142248 2 24 1.6196013158772615 2 25 0.9941514465707613 2 26 1.5508756777880492 2 27 0.4835476066749038 2 28 1.3989018710260837 2 29 1.4832569767173638 2 30 1.2613535135845453 2 31 -1.40369310516596 2 32 -11.171157984224795 2 33 -1.7304481262396152 2 34 -0.7688055189417611 2 35 -1.3284346397567859 2 36 3.3450078479996854 2 37 3.62159728165501 2 38 5.00959662954855 2 39 7.264319065926074 2 40 10.266005421189746 2 41 6.531974383470498 2 42 2.2496147728299363 2 43 -2.010073841690687 2 44 -8.009490629603354 2 45 2.5944979085570843 2 46 -9.887302662291415 2 47 -9.677696948815282 2 48 -7.631537992279599 2 49 -11.585160731477792 2 50 -7.692734529110417 2 51 -7.1501122237209245 2 52 -7.9469138434121565 2 53 -8.368933894568007 2 54 -13.810765868990995 2 55 -7.420521072994694 2 56 -6.455968026592691 3 1 1.5469142972197716 3 2 3.1374639585247337 3 3 3.570981647149445 3 4 5.235394305377014 3 5 4.751724352939824 3 6 2.6571188958881677 3 7 2.638390610231639 3 8 2.5350862978693627 3 9 2.765999782722055 3 10 2.5332983982320343 3 11 1.1391047302812751 3 12 2.268290429547065 3 13 -5.247441950524756 3 14 -8.439037780731546 3 15 -3.5945013398397805 3 16 2.2560650413126737 3 17 2.578435130962262 3 18 2.682183351476887 3 19 1.4029197550999228 3 20 0.9322751915652319 3 21 3.3648198847647492 3 22 3.644470466931253 3 23 3.193498473030118 3 24 4.096719435852528 3 25 3.1852460662368074 3 26 2.435538633879581 3 27 3.1066150154565975 3 28 2.583193769603696 3 29 3.563343556803943 3 30 3.1225161604614797 3 31 3.4704565546979893 3 32 3.0357065797826266 3 33 3.2741900926843215 3 34 3.3270510054861973 3 35 3.528708217386068 3 36 5.34129727286054 3 37 6.785790231562242 3 38 6.834581168818804 3 39 9.057145287323728 3 40 12.587588599915783 3 41 11.3445903389436 3 42 8.707870802514542 3 43 3.194317005557302 3 44 3.6831500630373384 3 45 9.53698399390031 3 46 1.3090486054884245 3 47 -4.009484209173969 3 48 0.8938176045045694 3 49 -9.496416142733205 3 50 -0.2156164091351549 3 51 -1.74813325031338 3 52 -1.465775933702819 3 53 -2.1775300603070313 3 54 -6.198520971031812 3 55 -1.796835235023142 3 56 0.7830548552873324 4 1 1.777416035918236 4 2 3.088780401644449 4 3 4.257022382370508 4 4 5.49321844844706 4 5 4.8371689629746015 4 6 2.5046102023958667 4 7 2.4463886231476835 4 8 2.8768647380084573 4 9 3.1957066878387934 4 10 2.574033619295129 4 11 3.978150929983215 4 12 3.572811045543091 4 13 5.073223722599938 4 14 3.1762130886176827 4 15 6.412701839494545 4 16 5.888951280756288 4 17 4.933378002298575 4 18 5.41119477720963 4 19 4.162482596629978 4 20 3.690844491118142 4 21 2.787820381535738 4 22 3.1673219324056703 4 23 2.375813425836874 4 24 4.07560666883812 4 25 3.0764532197390437 4 26 1.7472624292099326 4 27 3.027876813767577 4 28 2.4962588466031974 4 29 2.3052710282396163 4 30 2.0328489970238195 4 31 2.342041254151539 4 32 3.3658109016902245 4 33 2.6385716128035455 4 34 2.245580563360015 4 35 2.053298381320495 4 36 4.551431401027454 4 37 5.1144042405041255 4 38 6.502738148451197 4 39 8.70493465642457 4 40 11.315854869165662 4 41 4.456363811372807 4 42 7.8956502361956105 4 43 7.773800363729187 4 44 11.020954335267842 4 45 8.75307937393012 4 46 7.412128585617584 4 47 7.5491347674777955 4 48 7.5999775647628915 4 49 5.553012570629935 4 50 6.664910168112733 4 51 5.18678975018339 4 52 5.538198234205776 4 53 4.571848975957252 4 54 -1.2968816267695153 4 55 4.709622787828323 4 56 3.3138546565789397 5 1 2.354023854329327 5 2 3.550634121694891 5 3 4.428207754375556 5 4 5.63224745223016 5 5 5.2016078028232755 5 6 3.1215711103403834 5 7 2.9000456218866475 5 8 3.142312405095474 5 9 2.6197293864516595 5 10 3.3940210089546454 5 11 4.387356478533029 5 12 6.08006199131863 5 13 6.67505222196942 5 14 6.647209305515542 5 15 6.79069679535835 5 16 7.019468304715936 5 17 6.355824408351538 5 18 5.8074747267682625 5 19 4.601637703817872 5 20 5.4392681985582385 5 21 3.4772907472356067 5 22 2.284913357374144 5 23 2.5769483564800026 5 24 3.5658336549667453 5 25 2.9894418704326142 5 26 1.4978929462338897 5 27 2.6479903068318933 5 28 2.4707229071328385 5 29 2.6233668668272543 5 30 2.0741478620931773 5 31 1.790969375210807 5 32 4.0776646217406665 5 33 1.8248893933836214 5 34 1.9517596301948217 5 35 2.7210159096937616 5 36 3.78535321691648 5 37 4.934391630163642 5 38 5.759989094226736 5 39 6.88873036890881 5 40 7.698893961221145 5 41 3.9424924368456935 5 42 8.943884788528528 5 43 12.05154311278341 5 44 11.388861018748294 5 45 9.535235742152064 5 46 10.051472847912667 5 47 10.571518121828362 5 48 10.051743012429974 5 49 11.662092015774952 5 50 11.215036271517523 5 51 10.222729220549095 5 52 9.647277679350793 5 53 10.637738004961033 5 54 8.551479028968188 5 55 11.035915347727439 5 56 10.361143433375911 6 1 2.1232546235601006 6 2 3.2814033524641246 6 3 3.7551308312986293 6 4 5.439939759922467 6 5 5.163146264361735 6 6 3.352340341109617 6 7 3.1115840834251145 6 8 3.2384662512493136 6 9 2.888960155682426 6 10 2.7594056243392586 6 11 4.425818016994562 6 12 6.426215837472469 6 13 6.251975298892493 6 14 7.108747767054002 6 15 7.733004487666044 6 16 6.731006766254396 6 17 6.432747485274618 6 18 6.3074747267682625 6 19 4.9862530884332585 6 20 5.420037429327472 6 21 3.8619061318509935 6 22 4.496451818912611 6 23 3.269256048787696 6 24 3.585064424197519 6 25 3.662518793509534 6 26 2.6709698693108166 6 27 2.2249133837549664 6 28 2.951492137902065 6 29 2.5656745591349477 6 30 3.131840169785484 6 31 3.079430913672347 6 32 4.616126160202207 6 33 2.1518124703066945 6 34 2.9132980917332816 6 35 3.201785140462988 6 36 3.919968601531867 6 37 4.107468553240569 6 38 5.529219863457502 6 39 6.177191907370343 6 40 3.6412016535288387 6 41 3.0771078214610803 6 42 8.116961711605448 6 43 12.724620035860333 6 44 11.581168711055984 6 45 8.131389588305908 6 46 10.436088232528054 6 47 10.552287352597595 6 48 10.570973781660747 6 49 12.412092015774952 6 50 10.522728579209836 6 51 8.915036912856781 6 52 8.974200756273873 6 53 9.830045697268726 6 54 8.705325182814342 6 55 7.651299963112056 6 56 8.495758817991298 7 1 1.934793085098562 7 2 2.5677669888277634 7 3 3.601284677452476 7 4 4.708121578104283 7 5 4.145663746879215 7 6 1.5904522292215049 7 7 1.7626330344740637 7 8 2.056298419081486 7 9 1.9599391766614502 7 10 1.3999650648987014 7 11 3.157286548463098 7 12 5.971320732577368 7 13 5.7677095646267595 7 14 6.1276288859351205 7 15 6.22671078137234 7 16 5.680307465555096 7 17 5.411768464295598 7 18 4.8791530484465895 7 19 3.7726167247968974 7 20 3.4777297370197786 7 21 2.5496683696132294 7 22 1.9202280426888336 7 23 2.3692560487876975 7 24 1.8997497388828322 7 25 1.7852460662368088 7 26 2.0870537853947297 7 27 0.976311985153572 7 28 1.698345284755213 7 29 1.0936465871069743 7 30 1.0710010089463253 7 31 1.237123221364655 7 32 3.4266156706917172 7 33 2.1196446381388654 7 34 1.5573540357892242 7 35 1.910526399204251 7 36 -0.2950663635030999 7 37 -0.4233006775286654 7 38 -0.2260248917872545 7 39 2.499569529747969 7 40 -5.933623521296337 7 41 -10.528136933783676 7 42 1.2351435297872655 7 43 6.8337109449512425 7 44 5.671028850916127 7 45 2.6642567211730395 7 46 5.815109211549029 7 47 6.066273366583605 7 48 6.278666089353052 7 49 8.427826281509219 7 50 5.3510502575315115 7 51 7.4821697799896505 7 52 7.049375581448697 7 53 6.952772969995998 7 54 7.546933574422734 7 55 7.242558704370797 7 56 7.270933643166121 8 1 2.3347930850985605 8 2 3.720082597747144 8 3 4.563548828395874 8 4 5.822015231620583 8 5 5.641375582213698 8 6 3.4913098621546084 8 7 3.399682777184182 8 8 3.4333138564742782 8 9 3.523575540297813 8 10 3.704253229564223 8 11 5.087646754295001 8 12 6.941818159678569 8 13 7.021568912825732 8 14 7.605845009434265 8 15 7.344034966621052 8 16 7.285796316326966 8 17 6.859452855376212 8 18 6.5806967877261755 8 19 5.604177616735143 8 20 5.458861812491477 8 21 3.7476100505737833 8 22 4.529833531539602 8 23 3.5617088789763756 8 24 4.807125382107529 8 25 3.7804433218114184 8 26 4.028734745943616 8 27 4.4785418307796405 8 28 3.794743226436175 8 29 3.5156019901944546 8 30 3.2617385732687936 8 31 4.599387372308051 8 32 6.129188569491035 8 33 4.492886490625999 8 34 4.022877191878422 8 35 4.3022931230464465 8 36 2.0531326073373783 8 37 2.4899068696411497 8 38 3.7774056399451617 8 39 5.206945172972667 8 40 -1.1048070547440219 8 41 -9.622819609598427 8 42 6.182999447454506 8 43 10.234053998124484 8 44 9.826088885221445 8 45 6.655337338668755 8 46 9.674114357346628 8 47 9.692345407749986 8 48 10.501307598787015 8 49 12.209987516500643 8 50 9.90988387674249 8 51 10.834485388909034 8 52 10.666871293284032 8 53 10.438536263306464 8 54 11.348648840288945 8 55 11.258339150339921 8 56 11.650693505944854 9 1 2.5147930850985603 9 2 3.691403352464121 9 3 4.481284677452479 9 4 5.929939759922469 9 5 5.26930011051558 9 6 3.3577249564942306 9 7 3.2808148526558796 9 8 3.145389328172392 9 9 3.1835755402978094 9 10 3.336328701262339 9 11 4.922741093917644 9 12 6.973138914395548 9 13 7.080436837354032 9 14 7.55490161320785 9 15 7.15761987228143 9 16 7.074852920100547 9 17 7.488132100659229 9 18 6.688243957537495 9 19 5.136253088433257 9 20 4.837729737019778 9 21 3.544213824158689 9 22 4.205682588143375 9 23 3.229256048787697 9 24 4.094295193428287 9 25 3.652518793509536 9 26 2.792508330849273 9 27 3.267221076062661 9 28 2.9637998302097586 9 29 3.1118284052887972 9 30 3.896455554400873 9 31 2.9171232213646547 9 32 4.588433852509901 9 33 2.9287355472297776 9 34 2.940990399425587 9 35 1.728708217386071 9 36 0.7976609092241738 9 37 1.2766993224713339 9 38 2.0230660173036554 9 39 4.274114984293423 9 40 -0.5372598849327019 9 41 -2.659046024692767 9 42 4.87696171160545 9 43 10.124620035860332 9 44 9.181937941825218 9 45 6.220620357536678 9 46 9.091472847912666 9 47 9.455364275674516 9 48 8.978666089353055 9 49 12.940553554236494 9 50 9.681959348440603 9 51 8.145806143626015 9 52 9.085739217812332 9 53 10.10004569726873 9 54 9.341479028968187 9 55 10.024376886188982 9 56 8.447297279529757 10 1 1.3547930850985637 10 2 3.151403352464122 10 3 4.361284677452474 10 4 5.089939759922466 10 5 4.8493001105155855 10 6 2.8577249564942306 10 7 2.7608148526558836 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115 33 2.1176244361186605 115 34 1.8691385475737334 115 35 2.1583378470156944 115 36 4.940623872187135 115 37 5.376699322471335 115 38 6.720103054340694 115 39 8.198559428737866 115 40 10.176814189141375 115 41 10.16613916049242 115 42 5.95029504493878 115 43 0.0209163321566308 115 44 0.22860460849188513 115 45 5.826546283462605 115 46 -0.8744530780132607 115 47 -5.580191279881042 115 48 -2.8991116884247248 115 49 -10.410557556874618 115 50 -0.7358184293371757 115 51 1.7569172547371252 115 52 1.3153688474419631 115 53 0.7837494009724288 115 54 1.5422197697089288 115 55 0.49104355285564694 115 56 3.1147046869371664 116 1 1.3347930850985605 116 2 2.698070019130789 116 3 2.734618010785809 116 4 3.189939759922467 116 5 3.5426334438489135 116 6 2.917724956494233 116 7 2.1474815193225467 116 8 2.065389328172394 116 9 2.4902422069644814 116 10 2.769662034595669 116 11 -2.997258906082358 116 12 -2.0135277522711164 116 13 -7.359563162645966 116 14 -10.311765053458817 116 15 -4.882380127718569 116 16 -9.931813746566117 116 17 -11.118534566007437 116 18 -4.111756042462503 116 19 -5.563746911566742 116 20 -4.822270262980222 116 21 1.7708804908253555 116 22 1.4656825881433804 116 23 1.902589382121029 116 24 0.9876285267616183 116 25 1.0458521268428669 116 26 2.0658416641826065 116 27 1.2338877427293298 116 28 2.3104664968764226 116 29 2.945161738622126 116 30 2.383122221067538 116 31 -0.7295434453020135 116 32 -7.958232814156766 116 33 -0.004597786103559542 116 34 1.5209903994255853 116 35 1.1287082173860696 116 36 4.344327575890841 116 37 4.943365989138005 116 38 4.216399350636987 116 39 6.754114984293423 116 40 8.902740115067296 116 41 6.0476206419739 116 42 1.9836283782721154 116 43 -9.075379964139668 116 44 -0.6047287248414506 116 45 1.7006203575366783 116 46 -3.615193818754001 116 47 -8.591302390992151 116 48 -5.55466724398028 116 49 -3.4327797790968404 116 50 -4.324707318226064 116 51 -1.3541938563739855 116 52 -0.7475941155210002 116 53 -1.6866209693979393 116 54 -5.998520971031812 116 55 -3.1089564471443545 116 56 -0.9593693871369098 117 1 -0.1388911254277545 117 2 0.7419296682535972 117 3 0.33812678271563357 117 4 0.689939759922467 117 5 1.2461422157787396 117 6 0.8756196933363327 117 7 0.8018674842348261 117 8 0.7759156439618664 117 9 0.6814702771399155 117 10 -0.37419761452713374 117 11 -8.07620627450341 117 12 -9.141597927709714 117 13 -8.22272105738281 117 14 -9.018782597318467 117 15 -8.145538022455412 117 16 -12.23356813253103 117 17 -9.951867899340769 117 18 -4.685440252988819 117 19 -5.63216796419832 117 20 -3.5222702629802214 117 21 -0.774733544262368 117 22 -0.47115951711978 117 23 -0.5465334248965164 117 24 -3.224652174992766 117 25 -1.2453759433325686 117 26 0.6851399097966464 117 27 1.035642128694242 117 28 1.5911682512623884 117 29 1.543407352657212 117 30 0.27961344913771313 117 31 -0.09971888389850392 117 32 -12.612618779069049 117 33 -1.1396855054018076 117 34 0.1420430310045333 117 35 0.10765558580712309 117 36 1.1355556460662761 117 37 0.5345940593134415 117 38 -0.6853550353279232 117 39 2.596220247451317 117 40 0.050108536119928715 117 41 3.13884871214934 117 42 -1.540933025236658 117 43 -8.643801016771246 117 44 -3.701219952911625 117 45 -0.8888533266738499 117 46 -5.264316625771542 117 47 -10.86674098748338 117 48 -9.226597068541682 117 49 -3.2994464457635075 117 50 -3.431724862085712 117 51 -2.3541938563739855 117 52 -2.2616292032402967 117 53 -2.656796407994431 117 54 -9.619573602610759 117 55 -4.228254692758391 117 56 -2.0295448257333994 118 1 1.4886392389447138 118 2 2.608326429387205 118 3 3.2166692928370892 118 4 3.2668628368455472 118 5 4.009300110515582 118 6 2.5638788026480768 118 7 2.1885071603481876 118 8 2.2576970204800872 118 9 2.100498617220893 118 10 2.6055594704931053 118 11 -6.881874290697741 118 12 -11.439168777912144 118 13 -8.074947778030584 118 14 -0.9681753098690749 118 15 -16.305457050795493 118 16 -4.711300926053298 118 17 -9.874944822417692 118 18 -1.6348329655394274 118 19 -1.8022084500282816 118 20 -3.599193339903298 118 21 1.0734445933894534 118 22 1.496451818912611 118 23 1.730794510326156 118 24 -2.299550960417868 118 25 1.9894418704326142 118 26 2.82481602315697 118 27 2.9749133837549664 118 28 1.8361075225174517 118 29 2.5272130206734076 118 30 2.8241478620931773 118 31 -0.2090306247891931 118 32 -8.768489224413177 118 33 1.9979663164605412 118 34 1.0671442455794349 118 35 1.413323602001455 118 36 4.78535321691648 118 37 4.261314707086722 118 38 5.913835248072889 118 39 8.215653445831883 118 40 9.833509345836532 118 41 9.32710782146108 118 42 4.886192480836218 118 43 4.609235420475716 118 44 1.908091787979064 118 45 5.208312665228984 118 46 -2.9485271520873333 118 47 -8.678481878171638 118 48 -3.1597954491084863 118 49 -6.645600291917354 118 50 -1.6503483438670905 118 51 -0.046501548681678884 118 52 0.03189306396618008 118 53 -3.5353389181158903 118 54 -7.929290201801042 118 55 -3.906392344580251 118 56 -0.17731810508562518 119 1 1.030445259011607 119 2 1.835751178551078 119 3 2.992589025278562 119 4 3.689939759922467 119 5 3.117995762689496 119 6 1.5872901738855347 119 7 1.6851626787428344 119 8 1.5653893281723938 119 9 1.7844451055152035 119 10 1.6624156577840807 119 11 -8.279867601734534 119 12 -10.681643694300103 119 13 -1.0899979452546624 119 14 -1.6320549085312805 119 15 -9.708467084240308 119 16 -8.430364471203799 119 17 -6.734476594992945 119 18 -0.820451694636418 119 19 -1.3941816941754368 119 20 -1.9135746108063074 119 21 1.1520399111152102 119 22 0.004813022925986843 119 23 0.769256048787696 119 24 -3.9065743717891053 119 25 -0.69617685866438 119 26 0.9368561569362299 119 27 1.3193949891061365 119 28 0.5133650476010629 119 29 0.8248718835496618 119 30 0.8425425109226126 119 31 0.11538409092987223 119 32 -16.45243571270749 119 33 -0.5538731484223973 119 34 -0.5833574266613653 119 35 0.18088213042954493 119 36 2.62983482226765 119 37 3.028873235514812 119 38 3.5526312346949602 119 39 6.319332375597774 119 40 9.581000984632517 119 41 8.76356267095941 119 42 3.6604399724750145 119 43 2.9420113402081576 119 44 1.127155333129565 119 45 5.031055140145373 119 46 -1.6876575868699426 119 47 -10.024635724325485 119 48 -5.686551301951294 119 49 -6.169011663154812 119 50 -3.4797797819941785 119 51 -3.528106899852247 119 52 -4.183825999578971 119 53 -9.354736911426926 119 54 -6.807216623205724 119 55 -9.784318765984933 119 56 -5.85792011177459 120 1 0.5271007774062539 120 2 1.723711044771818 120 3 2.524361600529396 120 4 2.728401298384007 120 5 3.470838572054042 120 6 1.17926341803269 120 7 1.1885071603481876 120 8 1.4500047127877806 120 9 1.2928063095285793 120 10 0.7209440858777256 120 11 -9.612643521466975 120 12 -5.285322624065991 120 13 -8.459563162645967 120 14 -5.391252232945998 120 15 -7.382380127718569 120 16 -10.980531695284068 120 17 -5.836483283956152 120 18 -3.750217580924044 120 19 -2.456054603874435 120 20 -4.906885647595605 120 21 -0.6188630989182329 120 22 -1.1573943349335423 120 23 -0.8461285665969172 120 24 -5.991858652725561 120 25 -1.4720965911058528 120 26 0.901739100080043 120 27 2.0133749222165065 120 28 1.4130305994405248 120 29 1.8349053283657142 120 30 1.0933786313239509 120 31 -1.709030624789193 120 32 -9.537719993643947 120 33 -1.2328029143086852 120 34 -9.202086523651335 120 35 -1.5097533210754719 120 36 1.208430139993407 120 37 2.4920839378559485 120 38 0.9138352480728855 120 39 3.215653445831883 120 40 -2.6664906541634714 120 41 -3.095969101615843 120 42 -7.075345980702245 120 43 -7.1215338102935135 120 44 -8.01498513509786 120 45 -12.099379642463322 120 46 -15.871604075164257 120 47 -15.947712647402408 120 48 -12.621333910646946 120 49 -14.876369522686584 120 50 -11.88111757463632 120 51 -12.200347702527832 120 52 -12.160414628341513 120 53 -12.612261995038965 120 54 -14.275444047954888 120 55 -12.252546190734098 120 56 -9.677318105085627 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 363.0 5 12.0 6 14.0 7 18.0 8 68.0 9 81.0 10 46.0 11 52.0 12 57.0 13 75.0 14 95.0 15 131.0 16 229.0 17 269.0 18 282.0 19 382.0 20 520.0 21 615.0 22 766.0 23 951.0 24 1035.0 25 1128.0 26 1165.0 27 1208.0 28 1301.0 29 1345.0 30 1571.0 31 1817.0 32 2111.0 33 2509.0 34 2962.0 35 3450.0 36 3920.0 37 3594.0 38 1987.0 39 488.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 100.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 100.0 0.0 0.0 0.0 7 99.2571756288063 0.3932599612201983 0.3249856623972472 0.024578747576262393 8 99.4701914411339 0.46153426004314935 0.03550263538793457 0.03277166343501652 9 97.86397159246107 1.3493580988800875 0.4506965310024583 0.33597377765637804 10 51.02547996832073 30.59780976049376 8.460551110140099 9.916159161045417 11 37.624600595351886 28.765327580085753 16.424065324849114 17.186006499713248 12 38.51489745200317 12.363110030859984 19.4335964169648 29.68839610017205 13 28.972881448507525 12.843761094573559 23.095829805827893 35.08752765109102 14 22.167891386893217 15.88220832081296 29.622749747316085 32.327150544977734 15 26.44379394304161 17.142231827756955 28.353349930238288 28.06062429896315 16 32.97366695804196 16.876092657342657 24.721372377622377 25.428868006993007 17 30.036049814288834 21.2830456630981 23.32859951933581 25.35230500327725 18 31.454168032339126 24.259805528242108 22.716595651698896 21.569430787719874 19 29.686689065530338 27.345734655412603 18.51730448796744 24.450271791089623 20 27.177624211302614 27.25956679686433 21.152112752997734 24.41069623883532 21 26.76444881459631 21.97913252485524 28.381405003823883 22.875013656724573 22 25.379615468647586 22.167904741096788 27.61907362901464 24.833406161240987 23 28.606931206816505 22.23830461261163 26.413960728623316 22.740803451948548 24 26.632438484856763 24.488625501816095 23.030286478957862 25.848649534369283 25 28.516809132370213 24.294726493158915 21.937897697790643 25.25056667668023 26 22.170237215792497 22.796793345919177 28.257955074010233 26.775014364278093 27 21.723214773534394 18.10358957547943 30.926624050702074 29.246571600284106 28 20.802320490367777 24.050459719789842 28.590192644483363 26.55702714535902 29 25.459561334025295 23.1269290650351 29.51298789981153 21.900521701128074 30 23.784935661011392 19.886348113542606 35.32497336283911 21.003742862606888 31 21.828036462182805 21.036927540992583 38.542607648298706 18.59242834852591 32 29.88992379339543 15.345114856190762 33.86960203217612 20.895359318237688 33 27.072216293134115 17.497195085242044 36.926360725720386 18.504227895903455 34 20.44677767752167 20.189757471358654 44.28403467038526 15.079430180734422 35 20.77936273706072 24.495818986719133 40.04481609006941 14.680002186150734 36 22.142896219705673 24.889217134416544 36.12068493900104 16.847201706876742 37 22.339930470011772 23.183050012318304 37.310777148175525 17.1662423694944 38 24.81606082984601 23.43754273679604 36.60731380432701 15.139082629030934 39 20.895236006797873 17.726550079491258 32.791513623156625 28.586700290554244 40 14.886891509304306 13.985322008740827 27.231247079519527 43.89653940243534 41 14.988154922593797 15.869649055148477 30.155363340862763 38.986832681394965 42 17.96267921637299 22.22283025062931 40.81755499616942 18.99693553682828 43 10.859790535724077 12.140154630695838 32.00636069528979 44.993694138290294 44 13.664579213359701 13.628872775214237 27.897714787958687 44.80883322346737 45 19.20868423930876 19.10751394509461 39.64234933829159 22.041452477305043 46 18.065803137126306 21.380554189211345 39.132098158168006 21.421544515494343 47 16.67941642533195 19.802196601278617 38.80115840664445 24.717228566744986 48 19.202710456569854 22.517008661438837 40.33716768217711 17.943113199814203 49 15.514500301518558 14.716846664108326 27.454635162545916 42.314017871827204 50 18.836019372315103 19.807371330068133 40.93085615782417 20.425753139792597 51 19.39435461770348 24.017539051795012 38.25431625102768 18.33379007947383 52 17.481424615468978 23.49957502810298 39.1083815425109 19.910618813917143 53 19.71649837178119 21.153162028295434 40.612429193005504 18.517910406917878 54 16.029198676764086 20.403532274380076 35.77931487000027 27.787954178855568 55 19.980306884385 21.708924810590517 38.43986761850059 19.870900686523893 56 18.94768523585422 22.16263543832768 35.79128816898326 23.098391156834847 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 5.5 9 10.0 10 22.5 11 35.0 12 44.5 13 63.0 14 72.0 15 114.5 16 157.0 17 182.5 18 208.0 19 227.5 20 247.0 21 301.0 22 441.0 23 527.0 24 663.0 25 799.0 26 976.0 27 1153.0 28 1314.0 29 1497.0 30 1519.0 31 1476.0 32 1433.0 33 1267.5 34 1102.0 35 992.0 36 882.0 37 773.5 38 626.0 39 587.0 40 601.5 41 616.0 42 644.0 43 672.0 44 737.5 45 803.0 46 896.5 47 1009.5 48 1029.0 49 1061.5 50 1094.0 51 1154.0 52 1214.0 53 1392.5 54 1631.5 55 1692.0 56 1865.0 57 2038.0 58 2350.5 59 2663.0 60 2640.5 61 2618.0 62 2346.5 63 1980.0 64 1885.0 65 1667.5 66 1450.0 67 1253.5 68 1057.0 69 886.5 70 716.0 71 588.5 72 389.5 73 318.0 74 257.5 75 197.0 76 142.5 77 88.0 78 71.5 79 38.0 80 21.0 81 15.0 82 9.0 83 6.0 84 3.0 85 2.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.019116803670426306 10 0.0 11 0.0 12 0.0 13 0.0 14 0.027309719529180435 15 0.17478220498675479 16 0.024578747576262393 17 0.0027309719529180435 18 0.013654859764590218 19 0.021847775623344348 20 0.01638583171750826 21 0.013654859764590218 22 0.0027309719529180435 23 0.0 24 0.0 25 0.0 26 0.18570609279842695 27 0.030040691482098477 28 0.19936095256301717 29 0.01638583171750826 30 0.03823360734085261 31 0.23486358795095172 32 0.01638583171750826 33 0.20209192451593525 34 0.12016276592839391 35 0.06281235491711501 36 0.16112734522216457 37 0.23486358795095172 38 0.15293442936341045 39 0.36868121364393586 40 0.6445093808886583 41 0.8602561651691838 42 0.188437064751345 43 0.3905289892672802 44 0.5707731381598711 45 0.12289373788131196 46 0.06281235491711501 47 0.040964579293770655 48 0.04915749515252479 49 0.36868121364393586 50 0.19116803670426305 51 0.3468334380205915 52 0.3932599612201983 53 0.20209192451593525 54 0.10923887811672174 55 0.15293442936341045 56 0.188437064751345 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 36617.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.68924270147745 #Duplication Level Percentage of deduplicated Percentage of total 1 93.8254103266862 81.33653767375809 2 4.057587499606212 7.03498375071688 3 0.9356393535582649 2.4332960100499768 4 0.41583971269256215 1.441953191140727 5 0.18901805122389187 0.8192915858754131 6 0.15751504268657657 0.8192915858754131 7 0.100809627319409 0.6117377174536418 8 0.06300601707463063 0.43695551246688696 9 0.0504048136597045 0.3932599612201983 >10 0.1795671486626973 2.818363055411421 >50 0.01890180512238919 1.0541551738263648 >100 0.006300601707463062 0.8001747822049867 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCAAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC 157 0.4287625966081328 No Hit TCAAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 136 0.3714121855968539 No Hit TCAAAGGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC 85 0.2321326159980337 No Hit TCAAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTCCCTACCAAGCGC 67 0.18297512084550893 No Hit TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 63 0.17205123303383674 No Hit TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGACCACCCGACTGCTCTTC 60 0.16385831717508262 No Hit TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 59 0.16112734522216457 No Hit TCAAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 52 0.14201054155173828 No Hit TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 47 0.12835568178714807 No Hit TCAAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 40 0.10923887811672174 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0027309719529180435 0.0 0.0 0.0 0.0 6 0.0027309719529180435 0.0 0.0 0.0 0.0 7 0.0027309719529180435 0.0 0.0 0.0 0.0 8 0.0027309719529180435 0.0 0.0 0.0 0.0 9 0.0027309719529180435 0.0 0.0 0.0 0.0 10 0.0027309719529180435 0.0 0.0 0.0 0.0 11 0.005461943905836087 0.0 0.0 0.0 0.0 12 0.005461943905836087 0.0 0.0 0.0 0.0 13 0.005461943905836087 0.0 0.0 0.0 0.0 14 0.005461943905836087 0.0 0.0 0.0 0.0 15 0.005461943905836087 0.0 0.0 0.0 0.0 16 0.005461943905836087 0.0 0.0 0.0 0.0 17 0.00819291585875413 0.0 0.0 0.0 0.0 18 0.00819291585875413 0.0 0.0 0.0 0.0 19 0.010923887811672174 0.0 0.0 0.0 0.0 20 0.013654859764590218 0.0 0.0 0.0 0.0 21 0.019116803670426306 0.0 0.0 0.0 0.0 22 0.024578747576262393 0.0 0.0 0.0 0.0 23 0.03004069148209848 0.0 0.0 0.0 0.0 24 0.035502635387934564 0.0 0.0 0.0 0.0 25 0.040964579293770655 0.0 0.0 0.0 0.0 26 0.04642652319960674 0.0 0.0 0.0 0.0 27 0.04642652319960674 0.0 0.0 0.0 0.0 28 0.04642652319960674 0.0 0.0 0.0 0.0 29 0.05461943905836087 0.0 0.0 0.0 0.0 30 0.05461943905836087 0.0 0.0 0.0 0.0 31 0.06008138296419696 0.0 0.0 0.0 0.0 32 0.06008138296419696 0.0 0.0 0.0 0.0 33 0.06008138296419696 0.0 0.0 0.0 0.0 34 0.06008138296419696 0.0 0.0 0.0 0.0 35 0.06008138296419696 0.0 0.0 0.0 0.0 36 0.06008138296419696 0.0 0.0 0.0 0.0 37 0.062812354917115 0.0 0.0 0.0 0.0 38 0.062812354917115 0.0 0.0 0.0 0.0 39 0.062812354917115 0.0 0.0 0.0 0.0 40 0.06554332687003304 0.0 0.0 0.0 0.0 41 0.06554332687003304 0.0 0.0 0.0 0.0 42 0.0682742988229511 0.0 0.0 0.0 0.0 43 0.0682742988229511 0.0 0.0 0.0 0.0 44 0.07100527077586913 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAACAA 15 0.007967003 50.620834 39 TTCGTTT 20 3.8857397E-4 50.620834 38 ACCCGAT 15 0.007967003 50.620834 41 CAACAAA 20 3.8857397E-4 50.620834 40 TACGCCC 20 3.8857397E-4 50.620834 38 CCCTGGA 15 0.007967003 50.620834 41 GCTCCCC 15 0.007967003 50.620834 40 GCCCTTG 15 0.007967003 50.620834 38 GTACGCC 20 3.9394418E-4 50.480614 37 CCAACAC 25 1.9505092E-5 50.41079 43 TGCCTAG 35 4.782305E-8 50.410786 43 GCAGCAT 20 3.993732E-4 50.34116 42 CCCGATT 15 0.008143763 50.34116 42 TTGCCTA 35 4.8350557E-8 50.34116 42 TCCCCCA 20 3.993732E-4 50.34116 42 CGACTCC 35 4.8881702E-8 50.27173 44 GTCGCGG 15 0.008188405 50.271725 36 TATGCTT 15 0.008188405 50.271725 35 CAACACA 30 9.823852E-7 50.271725 44 ATGCTTG 15 0.008188405 50.271725 36 >>END_MODULE