FastQCFastQC Report
Fri 17 Jun 2016
SRR1526317_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526317_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102920
Sequences flagged as poor quality0
Sequence length56
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC2420.23513408472600078No Hit
AACGCCGGGATGAGATGGACTTAAATGAATTGACGGGTACCCACAGTTCTGAGTCG2190.21278663039253787No Hit
AACGCCGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG2170.21084337349397592No Hit
AACGCCGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG2010.19529731830547997No Hit
AACGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1880.18266614846482707No Hit
AACGCCGGGATGAGATGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG1840.17877963466770308No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC1780.17294986397201711No Hit
AACGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1700.16517683637776914No Hit
AACGCCGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG1650.16031869413136418No Hit
AACGCCGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG1640.15934706568208318No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1610.1564321803342402No Hit
AACGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGACCACCCGACTGCTCTTC1440.13991449669646328No Hit
AACGCCGGGATGAGATGGACTCAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG1030.10007773027594247No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGA150.00818635450.3407136
TAAGACG150.00820228550.3159735
TGATACC150.00826623950.21726634
AGAATTC150.00826623950.21726634
TCGCCTA301.0146778E-650.16805642
TATTATA150.00829835550.16805649
GAAATTA150.00833056450.11894247
TTCTAAT150.00833056450.11894247
CTGTACG150.00833056450.11894244
TCTAATC150.00833056450.11894248
CTTGCGA150.00833056450.11894245
TATACTA150.00833056450.11894244
GCGTCAC150.00833056450.11894243
ACGGGGC150.00842774849.97218333
ACGGATA204.1773866E-449.97218333
ACGGTCA355.2492396E-849.97218333
CGGTAGG252.0869198E-549.94780731
AACGTAA150.00844402749.94780330
CATATCT150.00844402749.94780327
TATGCTG150.00844402749.94780325