##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526317_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 102920 Sequences flagged as poor quality 0 Sequence length 56 %GC 55 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.15055382821609 39.0 38.0 39.0 33.0 40.0 2 36.95446949086669 39.0 38.0 39.0 33.0 40.0 3 36.06991838321026 39.0 37.0 39.0 30.0 40.0 4 35.716692576758646 38.0 35.0 39.0 29.0 40.0 5 36.376331130975515 39.0 38.0 39.0 32.0 40.0 6 36.42016129032258 39.0 38.0 39.0 32.0 40.0 7 36.0406723668869 38.0 36.0 39.0 32.0 40.0 8 36.15704430625729 38.0 36.0 39.0 32.0 40.0 9 36.28098523124757 38.0 37.0 39.0 32.0 40.0 10 35.6582491255344 38.0 35.0 39.0 29.0 40.0 11 34.63274387874077 38.0 33.0 39.0 27.0 40.0 12 32.23225806451613 36.0 30.0 39.0 15.0 40.0 13 31.652467936261175 36.0 29.0 39.0 13.0 40.0 14 31.168995336183443 36.0 27.0 39.0 12.0 40.0 15 31.7766226195103 36.0 29.0 39.0 14.0 40.0 16 31.51680917217256 36.0 29.0 39.0 13.0 40.0 17 31.85055382821609 36.0 29.0 38.0 16.0 40.0 18 32.03529926156238 36.0 29.0 38.0 18.0 40.0 19 32.81011465215702 36.0 30.0 38.0 23.0 40.0 20 32.58414302370773 36.0 30.0 38.0 21.0 40.0 21 34.20655849203265 38.0 33.0 39.0 24.0 40.0 22 34.01129032258064 38.0 33.0 39.0 23.0 40.0 23 34.07193937038476 38.0 33.0 39.0 24.0 40.0 24 33.78257870190439 38.0 33.0 39.0 23.0 40.0 25 33.991916051301985 38.0 33.0 39.0 24.0 40.0 26 34.21023124757093 38.0 34.0 39.0 25.0 40.0 27 34.145112708900115 38.0 34.0 39.0 24.0 40.0 28 33.97699183832103 38.0 33.0 39.0 24.0 40.0 29 33.90822969296541 38.0 33.0 39.0 23.0 40.0 30 33.59516129032258 38.0 33.0 39.0 23.0 40.0 31 33.23455110765643 37.0 33.0 39.0 20.0 40.0 32 32.14217839098329 36.0 31.0 39.0 11.0 40.0 33 33.30503303536727 37.0 33.0 39.0 21.0 40.0 34 32.92612708900116 37.0 33.0 39.0 18.0 40.0 35 32.996871356393314 37.0 33.0 39.0 18.0 40.0 36 30.873911776136804 35.0 30.0 38.0 4.0 39.0 37 30.666760590750098 35.0 30.0 38.0 4.0 39.0 38 29.858929265448893 34.0 29.0 38.0 4.0 39.0 39 28.236115429459776 33.0 24.0 37.0 4.0 39.0 40 24.410590750097164 30.0 10.0 36.0 4.0 39.0 41 21.010328410415855 24.0 8.0 33.0 4.0 36.0 42 24.39633696074621 29.0 17.0 33.0 4.0 36.0 43 21.0070248736883 25.0 6.0 33.0 4.0 36.0 44 21.677953750485813 26.0 7.0 32.0 4.0 36.0 45 23.88316167897396 29.0 13.0 33.0 4.0 36.0 46 23.90097162844928 29.0 4.0 35.0 4.0 38.0 47 23.246900505246792 29.0 4.0 35.0 4.0 38.0 48 23.337281383598913 29.0 4.0 35.0 4.0 38.0 49 20.93379323746599 24.0 4.0 34.0 4.0 38.0 50 23.154965021375826 29.0 4.0 35.0 4.0 38.0 51 24.564224640497475 31.0 4.0 37.0 4.0 39.0 52 24.372765254566655 31.0 4.0 37.0 4.0 39.0 53 24.075631558492034 31.0 4.0 36.0 4.0 39.0 54 23.24830936649825 30.0 4.0 36.0 4.0 39.0 55 23.372949863972018 30.0 4.0 36.0 4.0 39.0 56 22.95383793237466 30.0 4.0 37.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.4297549915278296 1 2 1.9706077829231248 1 3 2.9125619708594 1 4 2.8333744449296745 1 5 2.3823635969175925 1 6 2.56938267919616 1 7 2.819114921129305 1 8 2.783394491399015 1 9 2.5502499977540225 1 10 2.846745118428501 1 11 -2.769412185544631 1 12 -6.405056120380852 1 13 -19.650131695894572 1 14 -16.757205205724894 1 15 -10.91789250046692 1 16 -20.30906115575317 1 17 -21.293831034202917 1 18 -20.667332426964037 1 19 -5.981186364915377 1 20 -11.089416602179181 1 21 -8.222472159996745 1 22 -2.8380358363210902 1 23 -3.5705543852460764 1 24 -2.824375479277929 1 25 -2.9607277911692726 1 26 3.4784330792724987 1 27 4.099231625674641 1 28 3.6542701960952115 1 29 3.4177807510703744 1 30 3.854974126223077 1 31 2.1722225837813056 1 32 -11.557687426596193 1 33 1.6940385884643945 1 34 2.098248298797941 1 35 1.9736080631801727 1 36 5.260337613113176 1 37 5.5267794249656035 1 38 6.519615651366593 1 39 7.669659694406501 1 40 9.84008501735611 1 41 -3.5844118453757474 1 42 -8.857212985873888 1 43 -12.597554111263811 1 44 -14.583226711423638 1 45 -14.130363986853109 1 46 -17.350201862249598 1 47 -16.524869265099074 1 48 -16.17985365299495 1 49 -14.034148773284784 1 50 -16.25768055217577 1 51 -17.35944334328932 1 52 -17.321361641931006 1 53 -17.09624683299736 1 54 -16.7328293879044 1 55 -16.49711425911872 1 56 -16.037235907379085 2 1 1.8967835023519655 2 2 2.326338021865041 2 3 3.3393509158965173 2 4 3.4329127816942915 2 5 2.889818834299838 2 6 2.965539644696193 2 7 3.0951146715816122 2 8 3.0629502965022652 2 9 2.9590715087653194 2 10 3.239595576972391 2 11 -3.2722507905730147 2 12 -10.438495511484476 2 13 -12.928439762523805 2 14 -15.331102516848484 2 15 -8.582625172498862 2 16 -3.329212132108527 2 17 -2.7055847306281464 2 18 -9.905650475501062 2 19 -2.7195356069142527 2 20 -3.4308602355936166 2 21 3.6898118252799392 2 22 3.831750176530612 2 23 3.487278293024559 2 24 3.790634814564484 2 25 3.7350735688656655 2 26 2.9170131528890693 2 27 3.725721113477995 2 28 3.2304758233957287 2 29 3.8726438117728534 2 30 3.3783380291490204 2 31 1.0251766046185438 2 32 -7.280502324593574 2 33 0.4350704681824027 2 34 1.1305647252749509 2 35 0.46540418271352024 2 36 4.876034163116298 2 37 5.014458007534699 2 38 5.630227667088093 2 39 8.009917976270625 2 40 9.762226136577517 2 41 5.913061484214371 2 42 1.2007444662441493 2 43 -1.0544135535247143 2 44 -8.376850767821418 2 45 0.2444566507412489 2 46 -10.06609181171619 2 47 -10.476145077688754 2 48 -8.305313756557245 2 49 -9.643606630917823 2 50 -8.179073028312771 2 51 -7.8373895657610895 2 52 -7.618572946441578 2 53 -7.72298586849552 2 54 -13.411848665463822 2 55 -7.323241902764611 2 56 -6.706897146383387 3 1 1.8454647078510433 3 2 2.542942741761152 3 3 3.4560048946704214 3 4 3.3175126652642177 3 5 2.8749963901638793 3 6 3.2799904082087963 3 7 2.661860450991398 3 8 3.3121554373378785 3 9 3.0111067960341344 3 10 2.9172186122148815 3 11 2.2585360080477344 3 12 2.606374848105329 3 13 -4.599793486047158 3 14 -4.221837369743561 3 15 -2.831740751738483 3 16 3.349530423083806 3 17 3.0451653477382514 3 18 2.5652196154039615 3 19 1.2694035359812723 3 20 1.426602484392049 3 21 4.150292771050395 3 22 3.9512253455781305 3 23 3.4535135713372185 3 24 4.400101475260378 3 25 3.9199287029841514 3 26 3.099271000723917 3 27 3.07227970915217 3 28 3.189008804453451 3 29 3.33451296228111 3 30 2.798605818321022 3 31 3.325387074369111 3 32 2.3848955527966034 3 33 2.953542547354857 3 34 3.42943177108571 3 35 2.9445587212473967 3 36 4.873257753640683 3 37 5.124126181821559 3 38 6.299700597095708 3 39 8.188772483457942 3 40 10.561966407949157 3 41 8.569906229187726 3 42 7.321697401625457 3 43 2.032774660169256 3 44 3.888117213908018 3 45 6.648200325453534 3 46 -0.7617167277521943 3 47 -4.767488949436547 3 48 -1.6929363439917537 3 49 -7.8742725213839915 3 50 -1.1118570505240477 3 51 -3.087669445748549 3 52 -3.1008173600370306 3 53 -3.458734379533986 3 54 -6.066320028143505 3 55 -3.377561010731114 3 56 -0.9538632473193047 4 1 2.0909692561903697 4 2 2.659084057606293 4 3 3.6745719914901116 4 4 3.4919361896983148 4 5 2.982569904020451 4 6 3.4382303066644795 4 7 3.0235063151758723 4 8 3.0943287678505342 4 9 3.2225164286411427 4 10 3.1314489203736855 4 11 4.234419232079894 4 12 4.9210564643524215 4 13 5.622193679879082 4 14 4.374241891239446 4 15 7.03156225933267 4 16 7.181065575752875 4 17 6.213242352180817 4 18 6.6312234233159515 4 19 4.969742017047491 4 20 5.360774968702046 4 21 4.019201874780741 4 22 4.166090305736084 4 23 3.948013254011215 4 24 4.5677553783701725 4 25 4.0394195710470555 4 26 3.344775549063243 4 27 3.4067765711506155 4 28 3.166795984483066 4 29 3.596236395043263 4 30 3.8256490451738543 4 31 3.9563369368611774 4 32 4.666892591087272 4 33 3.6333253553373694 4 34 4.447202027062019 4 35 3.73149194175371 4 36 4.955445187531101 4 37 5.438067370736306 4 38 5.976733246415222 4 39 7.762497890692973 4 40 9.863073523103232 4 41 5.349717243925756 4 42 7.787496710559946 4 43 7.349395490858207 4 44 10.07872436432054 4 45 7.921065426645271 4 46 8.185607156318042 4 47 7.7221098094662075 4 48 7.765917051396443 4 49 4.870949959400157 4 50 7.522160296630599 4 51 5.8671997454227345 4 52 5.714005289987654 4 53 5.45311950947243 4 54 -0.008249370220934082 4 55 5.4772447152402535 4 56 4.785929433027636 5 1 2.659280037508573 5 2 3.037739666510703 5 3 4.110116877275068 5 4 3.6933766087293094 5 5 3.359697795770778 5 6 3.795716120719476 5 7 3.293257515525063 5 8 3.776199857622103 5 9 3.5399178545104917 5 10 3.563720126542748 5 11 4.786143370010926 5 12 7.734238480939066 5 13 7.644090959087578 5 14 7.503370364977254 5 15 8.006536797380097 5 16 8.110499592921506 5 17 7.322365006043757 5 18 6.643226915240874 5 19 6.027577023594848 5 20 6.414099572223066 5 21 4.374141493578932 5 22 5.103162908675124 5 23 4.370536169768528 5 24 5.055533641137522 5 25 4.06437228456371 5 26 3.6346053177232207 5 27 3.6539757563681334 5 28 3.8341901355088197 5 29 4.143086387768413 5 30 3.5963459751898625 5 31 4.4048310442378735 5 32 5.302422927185045 5 33 3.918208230355553 5 34 4.208223415102175 5 35 4.0000064186924575 5 36 5.0329222717737885 5 37 5.330035943411993 5 38 6.194396566653836 5 39 7.805492216314978 5 40 6.633333962503787 5 41 2.4701159053545325 5 42 9.550555054805546 5 43 12.203971367476857 5 44 11.96589154022626 5 45 10.332094089529015 5 46 11.059243123144753 5 47 10.912055975623637 5 48 10.579099067983087 5 49 10.818280091802311 5 50 10.88837592299463 5 51 9.55459971632336 5 52 9.25539915001966 5 53 9.985274318144043 5 54 9.386774636762915 5 55 10.352772141400592 5 56 8.830014985188264 6 1 2.362233624006464 6 2 2.954026586341925 6 3 3.9127329110303393 6 4 3.5802120517672833 6 5 3.4250986396526315 6 6 3.6430578928713757 6 7 3.23637987839426 6 8 3.3416850896896193 6 9 3.4606773481813775 6 10 3.4411040927874765 6 11 5.059476703344259 6 12 8.412550717225987 6 13 7.613458047695175 6 14 8.34826487974519 6 15 8.085355362780941 6 16 8.556237989545977 6 17 7.1789894786175985 6 18 7.400188940557335 6 19 6.244454660725648 6 20 6.52169450893193 6 21 4.410006472481889 6 22 4.876665018379761 6 23 4.655599460907773 6 24 5.308445033542583 6 25 4.0288448584033745 6 26 3.8827065835460033 6 27 3.970684617127631 6 28 3.4679454097704223 6 29 3.850259383549009 6 30 3.923350194599145 6 31 4.488290959849685 6 32 5.73769718878842 6 33 4.616773631199436 6 34 4.672949153498799 6 35 4.499078148650263 6 36 4.167858980634545 6 37 4.284044382230562 6 38 4.867307959058898 6 39 6.333762258509065 6 40 1.5734183506894404 6 41 1.8499471289832243 6 42 7.303128894467992 6 43 11.416292042582342 6 44 10.450448502251575 6 45 7.817157380668256 6 46 10.762618650570914 6 47 10.37889141866161 6 48 10.370744637603341 6 49 12.454398235262225 6 50 10.46078098628577 6 51 9.896625032779056 6 52 10.002740922171562 6 53 10.08392410717358 6 54 10.35588856081355 6 55 9.81057804857359 6 56 8.802251272108098 7 1 2.079893668156572 7 2 2.8689493236487706 7 3 3.6695099529729447 7 4 3.3077617206414516 7 5 3.294855814045569 7 6 3.485044647838265 7 7 3.2355410262971347 7 8 3.2933848689390643 7 9 3.1920680766581953 7 10 3.2692277130965266 7 11 4.2563420455076155 7 12 6.858599282347395 7 13 7.093458047695172 7 14 8.032459140230838 7 15 7.431006576908981 7 16 7.893633133033838 7 17 7.3558548207809515 7 18 6.786281655789118 7 19 5.843571658518147 7 20 6.02169450893193 7 21 4.507136715307496 7 22 4.31026325237535 7 23 3.8914935006428664 7 24 4.052683444138609 7 25 3.7462400018912376 7 26 2.9827065835460047 7 27 2.9251217032203414 7 28 2.724457551050776 7 29 3.2378973526439268 7 30 3.166175801663158 7 31 3.128864911284566 7 32 4.258580190995925 7 33 3.414477825459919 7 34 3.877893965860828 7 35 2.604332011784919 7 36 1.9656073250054078 7 37 1.9498280466897207 7 38 3.0127384226350564 7 39 4.16532958742739 7 40 -1.201857587500406 7 41 -8.104534107219864 7 42 4.007676355836647 7 43 9.044150762229144 7 44 7.921839230728395 7 45 5.283514996562296 7 46 7.164826156089678 7 47 7.490017246476182 7 48 8.090877087934466 7 49 10.203978809213663 7 50 7.823827343901662 7 51 9.672254171851904 7 52 9.342078670515932 7 53 9.503968257283955 7 54 10.182775978032094 7 55 8.567752441509569 7 56 8.892803148487786 8 1 2.2289002906731312 8 2 2.929728340727891 8 3 3.929487296995255 8 4 3.5915278412409677 8 5 3.5544846045649123 8 6 3.8093736823450612 8 7 3.1360290012012797 8 8 3.364141230040495 8 9 3.334887874497163 8 10 3.619349706822561 8 11 4.628248633168823 8 12 7.798778787401421 8 13 8.146089626642542 8 14 8.648440318341681 8 15 8.193162380324804 8 16 8.035799393054745 8 17 7.398814040021104 8 18 7.002820519504702 8 19 5.822963432655474 8 20 6.008536614195087 8 21 4.392462612832766 8 22 4.468419404344679 8 23 4.782178408276188 8 24 5.076602928279428 8 25 4.763406261912145 8 26 3.90375921512495 8 27 4.439368827653944 8 28 3.4841734799458592 8 29 4.100697980040238 8 30 3.9781747560026517 8 31 4.879431310726879 8 32 5.772609469490174 8 33 3.5569490697959267 8 34 4.468300030691779 8 35 4.2674114819835935 8 36 1.3975958227398095 8 37 2.034482978721787 8 38 2.5509921695852142 8 39 4.696481556754684 8 40 0.4672779998122465 8 41 -8.105666906104494 8 42 5.4003218769241315 8 43 8.90059028819638 8 44 7.926501133830524 8 45 6.033210012247206 8 46 8.838057247062142 8 47 8.369417734451083 8 48 8.972586742866497 8 49 11.246328059823629 8 50 9.182096775759454 8 51 10.523203980147475 8 52 10.499319869539978 8 53 11.254011826471828 8 54 10.400099087129341 8 55 10.426806118749028 8 56 9.284795131757221 9 1 1.9839338477201096 9 2 2.731520993500766 9 3 3.8102273181891775 9 4 3.2187355417001697 9 5 3.167827946140328 9 6 3.460910241864667 9 7 3.0224648895799433 9 8 3.2228484006515856 9 9 3.3369189589196324 9 10 3.239448611803134 9 11 4.497552765984082 9 12 6.859843782102946 9 13 6.858558718836115 9 14 8.024014320461074 9 15 7.587413528329041 9 16 7.563307340776397 9 17 6.805119232532586 9 18 6.720323168745253 9 19 5.482933407929227 9 20 5.4176676632943455 9 21 4.284726830423722 9 22 4.269461438961422 9 23 3.792377984397703 9 24 4.473679932871441 9 25 3.946115551915682 9 26 3.2578743687809038 9 27 3.352429583570583 9 28 3.78517136055342 9 29 3.289632985338713 9 30 3.878607465292653 9 31 3.7854274251740705 9 32 5.498413072457328 9 33 3.7680935417139807 9 34 3.9350520617762044 9 35 3.600465173258762 9 36 3.0976576383526684 9 37 3.0589884538189267 9 38 3.8608650060387646 9 39 5.329959126518016 9 40 -1.5335167723530638 9 41 -3.5921557792941776 9 42 5.770332473886338 9 43 9.269938575020824 9 44 9.294878032452917 9 45 6.364674159191747 9 46 9.214072789273377 9 47 9.152180009265638 9 48 9.533563429549648 9 49 11.164554834814798 9 50 9.08615011380255 9 51 9.439443824725366 9 52 9.598445620158138 9 53 9.664953189947628 9 54 9.544747621216235 9 55 9.768072455732426 9 56 8.368202055105858 10 1 2.413831797522448 10 2 2.9977708785793737 10 3 3.960038847103398 10 4 3.8443216408083813 10 5 3.8748703291503475 10 6 3.6049757010905523 10 7 3.2946447185769117 10 8 3.671639427589156 10 9 3.5582115947567203 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4.494364799665956 115 42 0.21991604306236923 115 43 -0.8734268329196659 115 44 -0.8876237869050527 115 45 -0.11224020969318715 115 46 -4.118505847421051 115 47 -6.768819424711884 115 48 -4.5519059648062985 115 49 -7.913151965540987 115 50 -3.1047611823889305 115 51 -1.9472303889076912 115 52 -1.4319578730091642 115 53 -2.344830912906744 115 54 -1.9407379452105467 115 55 -1.9283777747195856 115 56 0.16233159339323677 116 1 1.6807075195887933 116 2 2.010974377506585 116 3 2.8246204612311416 116 4 2.7775614493576413 116 5 2.4692753464799466 116 6 2.5541422302207764 116 7 2.651881886426388 116 8 2.7471469370791723 116 9 2.7079062638440234 116 10 2.921505699213178 116 11 0.8704807194085191 116 12 0.09118525537859412 116 13 -3.6173853257988036 116 14 -9.73599214836726 116 15 -5.769504074970062 116 16 -7.400709801618682 116 17 -9.258922167968748 116 18 -1.6974014208884505 116 19 -2.553938913571539 116 20 -2.785534406730722 116 21 2.5224562716786707 116 22 2.8319260625564695 116 23 2.938250063317412 116 24 1.9655534672775232 116 25 3.3193669467567872 116 26 3.789935499208653 116 27 3.488274978573415 116 28 3.7554956105736395 116 29 4.0100987409787265 116 30 3.553872282952554 116 31 1.3474877469982829 116 32 -6.729451405589089 116 33 1.9616732295930106 116 34 1.6075676273943813 116 35 1.9345801566823866 116 36 4.06159392039358 116 37 4.4800283179735345 116 38 4.550199525323961 116 39 7.153440973368507 116 40 7.795908310528798 116 41 5.482316606894873 116 42 1.7379883322189968 116 43 -9.3071617726787 116 44 0.43767741791422665 116 45 1.1166754529574128 116 46 -4.347421510071655 116 47 -7.527855569290196 116 48 -6.841062591312323 116 49 -5.093874857107252 116 50 -3.225243110099772 116 51 -1.2363870154137153 116 52 -0.9861747404790435 116 53 -2.248445370738068 116 54 -5.4347138488250035 116 55 -2.410305485562958 116 56 -0.8738129849200149 117 1 0.9533900865914973 117 2 1.0461354298793424 117 3 1.30851522395551 117 4 2.011640623195852 117 5 1.1053707484961706 117 6 1.7218334030754576 117 7 1.7627744362173914 117 8 1.2932497155399574 117 9 1.4925140828752532 117 10 1.7530768819031195 117 11 -9.030183160601318 117 12 -10.070850643318234 117 13 -8.00858276863136 117 14 -9.460850766513314 117 15 -7.884576610008171 117 16 -11.884850445828175 117 17 -9.767405079205535 117 18 -3.0753212635243017 117 19 -2.431735815464826 117 20 -4.182387123721131 117 21 0.7705506901689603 117 22 0.18673304559064974 117 23 0.4184566037649162 117 24 -4.29114680319211 117 25 -0.3995905157462829 117 26 0.288829032525598 117 27 0.9682356375357912 117 28 0.2012787431037566 117 29 0.22032741075989293 117 30 0.1614454326943786 117 31 -1.9278995163407906 117 32 -14.139581722776203 117 33 -2.684995076283556 117 34 -1.5028331594263733 117 35 -2.9341191302613048 117 36 -1.1884675499776982 117 37 -1.8560916721912086 117 38 -0.4788144899206941 117 39 0.6555309659920603 117 40 -1.4320238261813074 117 41 -2.5624338233977255 117 42 -4.146939132742894 117 43 -9.736224964220378 117 44 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118 27 2.9854214592328887 118 28 2.464436637840599 118 29 2.5809611379349704 118 30 2.7610694928447543 118 31 0.057062889674245554 118 32 -7.082653688404559 118 33 0.8069490697959267 118 34 1.0801421359549366 118 35 0.7345167451414909 118 36 3.7068063490556007 118 37 2.9489566629323107 118 38 4.458886906427317 118 39 5.913586819912577 118 40 7.36201484191751 118 41 6.40749098863235 118 42 0.9134797716609739 118 43 3.5782218671437462 118 44 1.9988695548831537 118 45 -0.039158408805427314 118 46 -5.819837489779962 118 47 -7.9463717392331255 118 48 -9.32346588871245 118 49 -6.648408782281633 118 50 -6.4429032242405455 118 51 -6.009690756694628 118 52 -6.099364340986336 118 53 -6.33151448931765 118 54 -9.310427228660135 118 55 -6.777141249672026 118 56 -5.563889078769096 119 1 1.1892177509905935 119 2 1.6629154752308182 119 3 2.757494815792242 119 4 2.662434273989504 119 5 1.996527211081208 119 6 1.8306769404904273 119 7 2.0620941641085437 119 8 1.810256518261042 119 9 1.8848043323083559 119 10 2.1385644102477954 119 11 -11.134491550623995 119 12 -9.869036584361318 119 13 -2.2557483015111757 119 14 -1.2749097234294133 119 15 -6.387977970552392 119 16 -7.433603280295294 119 17 -10.25720099757288 119 18 -2.4902872499188575 119 19 -3.3206247043537154 119 20 -2.3910039037664816 119 21 0.7909588534342689 119 22 0.2230142247289706 119 23 0.5295677148760234 119 24 -5.724253379155826 119 25 0.30789247745099146 119 26 2.998579599419024 119 27 3.2811608076038183 119 28 3.2012787431037566 119 29 2.462957796247423 119 30 2.082080353329303 119 31 0.23083064238937112 119 32 -8.799445668354434 119 33 0.43518632961213655 119 34 -0.18990798935834619 119 35 1.1225702121423211 119 36 2.1040494568250203 119 37 2.9443618425480196 119 38 3.970165101916045 119 39 5.18614321089002 119 40 6.205164382435473 119 41 5.993121732157828 119 42 1.2136050849441844 119 43 -0.5389460526557528 119 44 0.884416756219828 119 45 -0.4295092859984102 119 46 -8.440555952603688 119 47 -9.158568898798707 119 48 -8.952747425888724 119 49 -8.136712875848886 119 50 -7.211282505777723 119 51 -8.665914649760625 119 52 -9.042973363542727 119 53 -10.31480605155658 119 54 -9.641254296329308 119 55 -10.983072745077205 119 56 -8.41330428344746 120 1 1.1232664878562275 120 2 1.6284866802386446 120 3 2.453671878166489 120 4 2.468381065851787 120 5 2.364065775802871 120 6 2.0084100055474323 120 7 2.2505582821501307 120 8 2.2032813338210744 120 9 1.999268897477151 120 10 2.276503153820343 120 11 -11.143434094777806 120 12 -1.7974962311308218 120 13 -5.723443360755532 120 14 -4.4792468573440125 120 15 -7.284691585575864 120 16 -10.29991224519581 120 17 -9.48054103781433 120 18 -4.215304017189148 120 19 -3.6832448697907836 120 20 -5.41492520937793 120 21 -0.12050995944299814 120 22 -0.14295939476577502 120 23 0.007993827104954221 120 24 -7.424231022795443 120 25 -0.44730537633840584 120 26 1.4334108088981168 120 27 2.4568818002262205 120 28 2.497053390991077 120 29 1.721620885896428 120 30 1.054805593660177 120 31 -3.0103944861597043 120 32 -10.862490604638808 120 33 -2.3665127537770907 120 34 -12.619820799552844 120 35 -4.790687109565702 120 36 -3.921436794013342 120 37 -5.295298340774135 120 38 -4.609311759250961 120 39 -3.367176708627081 120 40 -5.112966625836382 120 41 -2.6710857348665407 120 42 -7.577810072668157 120 43 -3.523520163990426 120 44 -4.172368399156875 120 45 -10.819180647500756 120 46 -13.05334379074364 120 47 -13.150967736267965 120 48 -12.97376240465018 120 49 -13.7251322811697 120 50 -11.789641548925498 120 51 -12.504501727784323 120 52 -11.982611190504496 120 53 -12.23588809939919 120 54 -12.671435382848422 120 55 -12.687074533586037 120 56 -10.101880183290964 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 2889.0 5 387.0 6 35.0 7 198.0 8 388.0 9 362.0 10 259.0 11 206.0 12 221.0 13 259.0 14 354.0 15 481.0 16 627.0 17 789.0 18 936.0 19 1135.0 20 1427.0 21 1742.0 22 2046.0 23 2460.0 24 2657.0 25 2915.0 26 2964.0 27 3135.0 28 3401.0 29 3804.0 30 4205.0 31 5021.0 32 6064.0 33 7382.0 34 8699.0 35 10220.0 36 10866.0 37 8856.0 38 4533.0 39 997.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 0.0 100.0 7 99.38398756315586 0.4722114263505635 0.053439564710454725 0.09036144578313253 8 99.42576758647493 0.388651379712398 0.12825495530509132 0.057326078507578704 9 97.03095388502842 1.8076680110792558 0.6015841391709995 0.5597939647213178 10 52.59521958802954 33.58433734939759 6.475903614457831 7.344539448115041 11 39.58802953750486 21.27186164010882 22.568985619898953 16.57112320248737 12 42.22502914885348 10.992032646715897 19.650213758258843 27.132724446171785 13 26.827633113097548 15.88806840264283 22.787602020987173 34.49669646327245 14 27.39773953099641 13.313054548635069 26.202392637440596 33.08681328292792 15 26.660762598049708 18.599762539657824 26.25591217860132 28.483562683691144 16 32.37752721617419 15.918545878693624 27.60594867807154 24.097978227060654 17 36.41929715440833 19.64605038096719 20.787008241331055 23.147644223293423 18 36.16152979128609 21.621516576625595 21.20272843872673 21.014225193361575 19 29.698919297154408 29.206188773130147 18.691688695381746 22.403203234333695 20 29.272891618960607 24.65520425320983 20.432124563841885 25.639779563987673 21 28.058924129353237 22.352106654228855 28.712880907960198 20.87608830845771 22 25.58250257486543 21.110009910802773 29.603179230066658 23.704308284265142 23 27.482510687912942 25.237077341624563 25.766614846482703 21.513797123979792 24 28.45219588029537 25.257481539059462 22.59910610182666 23.6912164788185 25 26.641339305667564 26.113739931402364 22.18346466638813 25.06145609654194 26 25.98919550299314 19.433493940721274 28.43724144643987 26.14006910984572 27 26.86876148081914 17.30894459077258 28.262496476785664 27.559797451622625 28 21.025121910861504 27.080271367250997 25.45381987366044 26.44078684822706 29 25.174709627253726 26.99324488506585 25.50420372260291 22.327841765077512 30 25.9411907654921 18.88699878493317 35.044471445929524 20.1273390036452 31 26.40645846114893 18.035389095016896 34.914838295013 20.643314148821176 32 34.321309181372925 15.42214102464433 28.615019824302262 21.64152996968048 33 29.39486879898739 21.96582444866365 30.539895818119856 18.099410934229102 34 23.30441717075448 19.125513002547994 35.313053118860985 22.257016707836538 35 26.348231039209775 23.997394440825612 33.33560185500258 16.318772664962033 36 27.245034014929587 26.765223992447616 29.246026725320927 16.74371526730187 37 29.083222940855606 24.786682770981063 28.436452894880386 17.69364139328294 38 26.128589640038534 22.99802454238476 32.944404978542444 17.928980839034264 39 21.811249366150488 22.690837461481454 26.25209657916293 29.24581659320513 40 15.246930476264955 13.546961758035506 21.24227731289591 49.963830452803634 41 18.08677418722687 16.224107860236337 23.49742303396108 42.19169491857571 42 19.07080464695056 21.015473605281866 31.910293988762405 28.003427759005174 43 12.540343418781752 13.670446678432482 24.055890870442777 49.73331903234299 44 14.568359375 12.693359374999998 23.1142578125 49.6240234375 45 22.03112840466926 23.919260700389106 30.819066147859925 23.230544747081712 46 25.416889493898587 21.011230492488696 31.362730322329718 22.209149691283 47 17.96693524089067 18.597712097503134 35.29920594038236 28.13614672122384 48 21.541392482576956 19.713066552619047 38.337496719510895 20.408044245293105 49 16.237957798666095 12.75010725847342 23.522758297905536 47.48917664495495 50 20.462888133744876 18.50773589865924 37.779811690018796 23.249564277577093 51 23.89507340182871 20.073889224650536 33.86232039459575 22.168716978924998 52 18.828509011469144 24.716197238043225 34.69708200046813 21.758211750019505 53 23.57459983642949 19.187405070685827 35.47338084667212 21.764614246212563 54 17.350234449481487 17.2636535206335 35.41062707940152 29.975484950483487 55 20.773153735352512 19.324950363997353 32.34028886207031 27.561607038579826 56 25.02287906225052 20.3205015869307 30.00272601592772 24.653893334891055 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 1.0 10 1.5 11 2.0 12 4.0 13 15.5 14 25.0 15 45.0 16 65.0 17 95.5 18 126.0 19 172.5 20 219.0 21 270.0 22 420.0 23 519.0 24 590.0 25 661.0 26 842.5 27 1024.0 28 1234.5 29 1611.0 30 1777.0 31 1967.5 32 2158.0 33 2211.5 34 2265.0 35 2254.0 36 2243.0 37 2118.0 38 1874.0 39 1755.0 40 1664.0 41 1573.0 42 1579.5 43 1586.0 44 1644.5 45 1703.0 46 1865.5 47 2348.5 48 2669.0 49 3215.5 50 3762.0 51 4083.0 52 4404.0 53 4329.5 54 4444.5 55 4634.0 56 4894.0 57 5154.0 58 5483.5 59 5813.0 60 6385.0 61 6957.0 62 7632.5 63 8378.5 64 8449.0 65 7540.0 66 6631.0 67 5956.5 68 5282.0 69 4672.0 70 4062.0 71 3577.0 72 2682.5 73 2273.0 74 1897.5 75 1522.0 76 1251.5 77 981.0 78 799.0 79 472.0 80 327.0 81 231.5 82 136.0 83 101.5 84 67.0 85 47.0 86 27.0 87 14.0 88 0.5 89 0.0 90 0.5 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.024290711232024875 10 0.0 11 0.0 12 0.0 13 0.0 14 0.020404197434900895 15 0.16129032258064516 16 0.0388651379712398 17 0.023319082782743878 18 0.0038865137971239795 19 0.023319082782743878 20 0.03206373882627283 21 0.007773027594247959 22 0.0019432568985619898 23 0.0 24 0.0 25 9.716284492809949E-4 26 0.17975126311698406 27 0.030120481927710847 28 0.17586474931986007 29 0.03400699572483482 30 0.043723280217644774 31 0.22638942868247183 32 0.015546055188495918 33 0.20890011659541388 34 0.09133307423241353 35 0.061212592304702676 36 0.16614846482705012 37 0.2487368830159347 38 0.15448892343567822 39 0.3614457831325301 40 0.6062961523513408 41 0.8375437232802176 42 0.22250291488534785 43 0.3527011270890012 44 0.5052467936261174 45 0.1165954139137194 46 0.07287213369607462 47 0.030120481927710847 48 0.0378935095219588 49 0.35367275553828215 50 0.21084337349397592 51 0.32452390205985227 52 0.37310532452390205 53 0.20598523124757095 54 0.12242518460940535 55 0.16712009327633112 56 0.20015546055188493 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 102920.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.42013214146911 #Duplication Level Percentage of deduplicated Percentage of total 1 92.65164927320164 77.2901282549553 2 4.714871412597838 7.8663039253789355 3 1.1798825941110698 2.952778857364944 4 0.49268542676108834 1.6439953361834434 5 0.2876910175177041 1.1999611348620287 6 0.15840477077897877 0.7928488146132919 7 0.11530935519940366 0.6733385153517295 8 0.07454342154304883 0.4974737660318694 9 0.05241334327245621 0.39350952195880295 >10 0.24110137905329854 3.405557714729887 >50 0.01630637346254193 1.0143801010493587 >100 0.015141632500931793 2.269724057520404 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AACGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC 242 0.23513408472600078 No Hit AACGCCGGGATGAGATGGACTTAAATGAATTGACGGGTACCCACAGTTCTGAGTCG 219 0.21278663039253787 No Hit AACGCCGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG 217 0.21084337349397592 No Hit AACGCCGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG 201 0.19529731830547997 No Hit AACGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 188 0.18266614846482707 No Hit AACGCCGGGATGAGATGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG 184 0.17877963466770308 No Hit AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 178 0.17294986397201711 No Hit AACGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 170 0.16517683637776914 No Hit AACGCCGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG 165 0.16031869413136418 No Hit AACGCCGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG 164 0.15934706568208318 No Hit AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 161 0.1564321803342402 No Hit AACGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGACCACCCGACTGCTCTTC 144 0.13991449669646328 No Hit AACGCCGGGATGAGATGGACTCAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG 103 0.10007773027594247 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 9.71628449280995E-4 0.0 0.0 0.0 0.0 11 0.00194325689856199 0.0 0.0 0.0 0.0 12 0.0029148853478429848 0.0 0.0 0.0 0.0 13 0.0058297706956859695 0.0 0.0 0.0 0.0 14 0.006801399144966964 0.0 0.0 0.0 0.0 15 0.012631169840652934 0.0 0.0 0.0 0.0 16 0.013602798289933929 0.0 0.0 0.0 0.0 17 0.014574426739214923 0.0 0.0 0.0 0.0 18 0.018460940536338902 0.0 0.0 0.0 0.0 19 0.020404197434900895 0.0 0.0 0.0 0.0 20 0.023319082782743878 0.0 0.0 0.0 0.0 21 0.026233968130586864 0.0 0.0 0.0 0.0 22 0.027205596579867857 0.0 0.0 0.0 0.0 23 0.029148853478429847 0.0 0.0 0.0 0.0 24 0.032063738826272836 0.0 0.0 0.0 0.0 25 0.035950252623396815 0.0 0.0 0.0 0.0 26 0.0388651379712398 0.0 0.0 0.0 0.0 27 0.04080839486980179 0.0 0.0 0.0 0.0 28 0.04178002331908278 0.0 0.0 0.0 0.0 29 0.04275165176836378 0.0 0.0 0.0 0.0 30 0.04275165176836378 0.0 0.0 0.0 0.0 31 0.043723280217644774 0.0 0.0 0.0 0.0 32 0.043723280217644774 0.0 0.0 0.0 0.0 33 0.043723280217644774 0.0 0.0 0.0 0.0 34 0.043723280217644774 0.0 0.0 0.0 0.0 35 0.043723280217644774 0.0 0.0 0.0 0.0 36 0.043723280217644774 0.0 0.0 0.0 0.0 37 0.04469490866692577 0.0 0.0 0.0 0.0 38 0.04566653711620676 0.0 0.0 0.0 0.0 39 0.04566653711620676 0.0 0.0 0.0 0.0 40 0.04566653711620676 0.0 0.0 0.0 0.0 41 0.04566653711620676 0.0 0.0 0.0 0.0 42 0.04858142246404975 0.0 0.0 0.0 0.0 43 0.049553050913330746 0.0 0.0 0.0 0.0 44 0.050524679362611735 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGACGA 15 0.008186354 50.34071 36 TAAGACG 15 0.008202285 50.31597 35 TGATACC 15 0.008266239 50.217266 34 AGAATTC 15 0.008266239 50.217266 34 TCGCCTA 30 1.0146778E-6 50.168056 42 TATTATA 15 0.008298355 50.168056 49 GAAATTA 15 0.008330564 50.118942 47 TTCTAAT 15 0.008330564 50.118942 47 CTGTACG 15 0.008330564 50.118942 44 TCTAATC 15 0.008330564 50.118942 48 CTTGCGA 15 0.008330564 50.118942 45 TATACTA 15 0.008330564 50.118942 44 GCGTCAC 15 0.008330564 50.118942 43 ACGGGGC 15 0.008427748 49.972183 33 ACGGATA 20 4.1773866E-4 49.972183 33 ACGGTCA 35 5.2492396E-8 49.972183 33 CGGTAGG 25 2.0869198E-5 49.947807 31 AACGTAA 15 0.008444027 49.947803 30 CATATCT 15 0.008444027 49.947803 27 TATGCTG 15 0.008444027 49.947803 25 >>END_MODULE