FastQCFastQC Report
Fri 17 Jun 2016
SRR1526316_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526316_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57631
Sequences flagged as poor quality0
Sequence length56
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA1630.2828338914820149No Hit
AAAACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC950.16484183859381235No Hit
AAAACGGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC930.1613714840971005No Hit
AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA710.12319758463327028No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC710.12319758463327028No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAACC451.09139364E-1050.98130438
TTAATTT451.09139364E-1050.98130438
TAAACCA150.00776917250.981339
TTAGTTT150.00776917250.981338
TTATTTA150.00793477950.71036537
TTTAGTT150.00793477950.71036537
TTATTTC251.8970106E-550.7103637
CCGGACA150.00807477950.48677436
AAGACCA203.9563474E-450.48677436
TAAGACC203.9736225E-450.4422935
TCCTTCA150.0081312950.3978942
CCAACAC451.2187229E-1050.3978943
CCTTCTA150.0081312950.3978943
AGAATAT150.00818809550.3093244
CTTCTAT251.9884441E-550.3093144
TTCAAGC150.00824519450.22105434
GTAAGAC150.00824519450.22105434
TAGAGCG309.994146E-750.22105434
AAGGAAC150.00824519450.22105434
TGATACA252.0092504E-550.2210534