##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526316_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 57631 Sequences flagged as poor quality 0 Sequence length 56 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.31981051864448 38.0 37.0 39.0 33.0 40.0 2 37.09394249622599 39.0 38.0 39.0 33.0 40.0 3 36.760649650361785 39.0 37.0 39.0 32.0 40.0 4 35.61539796290191 39.0 36.0 39.0 27.0 40.0 5 36.075532265620936 39.0 37.0 39.0 29.0 40.0 6 35.993302215821345 38.0 36.0 39.0 29.0 40.0 7 36.173292151793305 38.0 36.0 39.0 31.0 40.0 8 36.05290555430237 38.0 36.0 39.0 30.0 40.0 9 36.00091964394163 38.0 36.0 39.0 30.0 40.0 10 35.70678974857282 38.0 35.0 39.0 29.0 40.0 11 34.36233971300168 38.0 33.0 39.0 27.0 40.0 12 32.207405736495986 36.0 29.0 39.0 16.0 40.0 13 31.91726674879839 36.0 29.0 39.0 14.0 40.0 14 31.58629904044698 36.0 28.0 39.0 13.0 40.0 15 32.020561850393015 36.0 29.0 39.0 15.0 40.0 16 31.757109888775137 36.0 29.0 39.0 15.0 40.0 17 31.93501761204907 36.0 29.0 38.0 16.0 40.0 18 32.01004667626798 36.0 29.0 38.0 17.0 40.0 19 32.94388436778817 36.0 30.0 38.0 23.0 40.0 20 33.00170047370339 36.0 30.0 38.0 22.0 40.0 21 34.51310926411133 38.0 34.0 39.0 25.0 40.0 22 34.27405389460533 38.0 33.0 39.0 24.0 40.0 23 34.418073606218876 38.0 33.0 39.0 25.0 40.0 24 34.09489684371258 38.0 33.0 39.0 23.0 40.0 25 34.344311221391266 38.0 33.0 39.0 25.0 40.0 26 34.81402370252121 38.0 35.0 39.0 27.0 40.0 27 34.717599902830074 38.0 35.0 39.0 26.0 40.0 28 34.67101039371172 38.0 35.0 39.0 27.0 40.0 29 34.56013256754177 38.0 35.0 39.0 26.0 40.0 30 34.41474206590203 38.0 35.0 39.0 25.0 40.0 31 34.111762766566606 38.0 34.0 39.0 23.0 40.0 32 33.08578716315872 38.0 33.0 39.0 15.0 40.0 33 34.155315715500336 38.0 34.0 39.0 24.0 40.0 34 33.84683590428762 38.0 34.0 39.0 22.0 40.0 35 33.86939320851625 38.0 34.0 39.0 23.0 40.0 36 31.489545557078657 35.0 31.0 38.0 12.0 39.0 37 31.25404730093179 35.0 30.0 38.0 9.0 39.0 38 30.605524804358765 35.0 30.0 38.0 4.0 39.0 39 28.75560028456907 33.0 26.0 38.0 4.0 39.0 40 24.25588658881505 30.0 10.0 37.0 4.0 39.0 41 20.834325276326975 24.0 8.0 32.0 4.0 36.0 42 24.999323280873142 30.0 19.0 33.0 4.0 37.0 43 20.82327219725495 24.0 6.0 32.0 4.0 36.0 44 21.91888046363936 27.0 7.0 33.0 4.0 36.0 45 24.608544012770906 30.0 17.0 33.0 4.0 37.0 46 24.83035172042824 30.0 5.0 36.0 4.0 38.0 47 23.98865194079575 30.0 4.0 35.0 4.0 38.0 48 24.013343513039857 29.0 4.0 35.0 4.0 38.0 49 21.1355173430966 25.0 4.0 35.0 4.0 38.0 50 23.817979906647466 29.0 4.0 35.0 4.0 38.0 51 25.382155437177907 32.0 4.0 38.0 4.0 39.0 52 25.154274609151326 32.0 4.0 38.0 4.0 39.0 53 24.771043362079435 31.0 4.0 38.0 4.0 39.0 54 23.850358314101786 31.0 4.0 38.0 4.0 39.0 55 24.064878277316026 31.0 4.0 38.0 4.0 39.0 56 23.278044802276554 30.0 4.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.1388816917330686 1 2 1.4392402211379292 1 3 2.032572103523023 1 4 3.79762522707631 1 5 2.8653514218921217 1 6 3.0576807806348256 1 7 2.9090933082711388 1 8 2.983633889806441 1 9 2.9207541779648594 1 10 3.2563641276671618 1 11 -3.7439245489881223 1 12 -7.528152708103896 1 13 -20.212170920974767 1 14 -17.45768203968842 1 15 -10.864573142786838 1 16 -20.604038765145454 1 17 -20.0413475652603 1 18 -19.965825896186296 1 19 -6.837422028933126 1 20 -10.487800237408507 1 21 -6.180208064419585 1 22 -3.9491452518820687 1 23 -3.7377294746834586 1 24 -3.9826673673797686 1 25 -3.811490230172801 1 26 2.273218201558443 1 27 2.205872217337088 1 28 2.0341029268784325 1 29 1.8880050583048487 1 30 1.7934217043692087 1 31 0.4204289477370935 1 32 -10.684296873488357 1 33 -0.30314735934378234 1 34 0.05471104192862697 1 35 0.2723112137341559 1 36 3.684489415190253 1 37 3.576539507864304 1 38 4.32115163716616 1 39 6.572487294761736 1 40 8.583133518273328 1 41 -0.9776311421575983 1 42 -5.365844052416104 1 43 -12.419400198068718 1 44 -14.907364978729017 1 45 -14.123820544375327 1 46 -17.621975373026853 1 47 -16.793499397326364 1 48 -16.056915784751244 1 49 -13.84475208516669 1 50 -16.061931695836947 1 51 -17.268933466985153 1 52 -17.495393510887457 1 53 -17.329074733215105 1 54 -17.364726848843603 1 55 -16.561551613485495 1 56 -15.738532647905451 2 1 1.3586619115132947 2 2 1.795284177181884 2 3 2.1007039716548874 2 4 3.6218010512521346 2 5 3.0972195537602545 2 6 3.354384077338125 2 7 2.999203198381032 2 8 3.2111064172789696 2 9 3.107567364778042 2 10 3.13218830349134 2 11 -2.961506966570539 2 12 -10.696284576235765 2 13 -11.753929162733009 2 14 -14.224715006721388 2 15 -5.800836879050575 2 16 -3.558983820090507 2 17 -1.445743169655902 2 18 -9.34055117091157 2 19 -2.1308286223397204 2 20 -2.4185694681777363 2 21 3.6494622652507474 2 22 3.3464591437223277 2 23 3.3084243714703874 2 24 4.2788710941586885 2 25 3.32807020938764 2 26 3.4853061136463594 2 27 3.70257551404039 2 28 3.669267762043269 2 29 3.2605325308323216 2 30 4.225289836237344 2 31 1.3522970796052292 2 32 -5.763417752609236 2 33 1.4309185747221562 2 34 1.8206451078626884 2 35 1.399783741206683 2 36 6.454819085519926 2 37 6.344671375996171 2 38 6.813459329473851 2 39 8.612047734322175 2 40 11.585331320471127 2 41 9.061929297402841 2 42 4.688002101430051 2 43 -1.9303892090577293 2 44 -8.079892451256491 2 45 2.9893662688114873 2 46 -9.519777570829051 2 47 -7.904488408315377 2 48 -6.781091608927067 2 49 -10.682114722529327 2 50 -7.0113822452874945 2 51 -6.262340060391743 2 52 -6.7008880163819615 2 53 -7.530173634314005 2 54 -13.754836738953493 2 55 -7.962650514584396 2 56 -7.409961219334022 3 1 1.589542142393526 3 2 2.199613181510891 3 3 2.620761691712609 3 4 4.52194535139644 3 5 3.424636581177282 3 6 3.635769358723401 3 7 3.1625509617287904 3 8 3.370269476442026 3 9 3.580583237793917 3 10 3.123963195266235 3 11 2.028103423039852 3 12 2.4351728552216656 3 13 -4.3806246894285366 3 14 -4.91735569936208 3 15 -1.7278210060347021 3 16 3.4085486474419575 3 17 3.513275589362859 3 18 2.628424298063898 3 19 1.6548856633745643 3 20 1.895571945963674 3 21 4.471540187328664 3 22 4.462043559306743 3 23 4.3225657856118005 3 24 4.953618568906165 3 25 4.558517539834973 3 26 3.896994425334668 3 27 4.08497089643577 3 28 3.677925770701272 3 29 3.6528846231844128 3 30 3.364828075775584 3 31 3.529209056517203 3 32 2.145384556193072 3 33 2.981856525660106 3 34 3.6686970559146417 3 35 3.305411446834391 3 36 6.099407830108671 3 37 6.4313957627205625 3 38 6.950833066847586 3 39 9.184919307193752 3 40 11.674508809648621 3 41 10.794396829870372 3 42 8.541537454965404 3 43 2.5782687996002807 3 44 5.156038284674246 3 45 8.125441304886522 3 46 1.5784908274393459 3 47 -1.6180526218795883 3 48 1.4531075252720669 3 49 -7.951667392081998 3 50 1.7063666724614244 3 51 0.6779196798679941 3 52 0.70041068491674 3 53 0.49392448978411707 3 54 -5.002022886139638 3 55 0.26144760951372703 3 56 2.960457251084449 4 1 2.083151707431661 4 2 2.4197739731002486 4 3 3.07417335940999 4 4 4.670780643088875 4 5 3.6829338394745363 4 6 3.6809146895830196 4 7 3.562468504503478 4 8 3.6580451927891744 4 9 3.544302058655596 4 10 3.6403515687974632 4 11 4.995635890572316 4 12 4.860858280907095 4 13 6.666479000532295 4 14 4.3671217279724885 4 15 7.184877406663709 4 16 7.308363118685005 4 17 7.019562952793077 4 18 6.751285563782304 4 19 5.726314234803134 4 20 5.552859103250832 4 21 4.100482673414007 4 22 5.209724449844778 4 23 4.061485595960185 4 24 4.713564971709715 4 25 4.401539597142744 4 26 3.4220408075239064 4 27 3.488289799754675 4 28 3.934573884492245 4 29 3.91767538797518 4 30 3.666106162767953 4 31 3.776786875523591 4 32 5.571276124941782 4 33 4.765612452273174 4 34 4.94513490378106 4 35 4.661008231002604 4 36 6.630329289601558 4 37 7.360997906608418 4 38 7.550194023351402 4 39 9.39776202003646 4 40 11.736351728634396 4 41 7.26192929740284 4 42 9.381879652450458 4 43 8.957365892983088 4 44 12.767046324253716 4 45 9.783243819831895 4 46 9.118997939375031 4 47 9.962858530460135 4 48 9.216867574746402 4 49 5.983191399919651 4 50 8.992699387365565 4 51 7.264190551853154 4 52 7.27462218769967 4 53 6.818805957522727 4 54 -0.0691224532392063 4 55 5.698573975211524 4 56 6.326773474543529 5 1 1.6046936575450346 5 2 2.4016333835310917 5 3 2.7975293684802907 5 4 4.602753432204516 5 5 3.0003941569348527 5 6 3.0337491567032018 5 7 2.715076214254047 5 8 3.2571381633107137 5 9 3.269472126682807 5 10 2.7179025892056288 5 11 4.29881049374692 5 12 6.951334471383284 5 13 7.158769249965406 5 14 6.48163419962782 5 15 7.027734549520858 5 16 7.515619354512669 5 17 6.052669528756798 5 18 6.732464702104302 5 19 5.565996774485676 5 20 4.825874976266704 5 21 3.5129543287428078 5 22 3.7448718421350264 5 23 3.7973132603592745 5 24 3.3566488719364713 5 25 3.6423559236733567 5 26 2.253560081900325 5 27 2.148607260072133 5 28 2.148632841408343 5 29 2.803389673689466 5 30 2.3173533283008325 5 31 2.8554716827798288 5 32 4.641344152152669 5 33 3.3626646064681864 5 34 3.2444546316722125 5 35 3.4013710427939827 5 36 5.280215910916748 5 37 5.141496772821569 5 38 5.880125996140514 5 39 7.593000115274556 5 40 6.269458304598114 5 41 3.8984372339107765 5 42 8.565779879207827 5 43 12.615642536974018 5 44 12.22169485033081 5 45 10.083017062462282 5 46 9.919904968853487 5 47 10.697098893271928 5 48 10.493511565676108 5 49 10.321059880645276 5 50 10.279093945188695 5 51 10.19004089198921 5 52 10.883238967745022 5 53 9.955540651400277 5 54 10.529290245173492 5 55 11.078619326685448 5 56 9.772578463205662 6 1 1.9875438990909302 6 2 2.6021164753185317 6 3 2.74076608345613 6 4 4.783912852494375 6 5 3.611505268045967 6 6 3.559353021437502 6 7 3.5358491611139513 6 8 3.6465101439870438 6 9 3.7665735759581693 6 10 3.7026851979012747 6 11 5.199052039640641 6 12 7.3919141815282075 6 13 7.29669195527941 6 14 8.160378160980478 6 15 7.555995419086074 6 16 7.900643509102036 6 17 7.984070494940372 6 18 6.718455040268552 6 19 6.274450880765869 6 20 6.111865314430958 6 21 4.922133072704163 6 22 5.395838025709907 6 23 4.875815675818217 6 24 5.196262398506519 6 25 4.7032254888907445 6 26 3.692448970789215 6 27 3.9184140233571583 6 28 3.917714967012209 6 29 3.8881722823851135 6 30 3.7308798983491442 6 31 4.233974098238768 6 32 5.418880384036726 6 33 4.412906152361906 6 34 4.338657530222939 6 35 2.9677961635669305 6 36 4.145191756327382 6 37 4.14560305301481 6 38 4.039546285995591 6 39 6.087203013825281 6 40 1.9291201403469067 6 41 -0.4042197709201396 6 42 5.514089057951789 6 43 10.470231908954695 6 44 8.9837721450168 6 45 5.997751361979187 6 46 9.05444603165542 6 47 9.898306622740524 6 48 9.398100937656785 6 49 11.301736209147691 6 50 8.613151916203186 6 51 8.147287268800802 6 52 7.8407268904503375 6 53 7.427279781835061 6 54 8.073734689617936 6 55 7.988281162434237 6 56 8.094076047746725 7 1 1.3294411322925157 7 2 2.2147646966624066 7 3 2.538690984641903 7 4 4.538359492810578 7 5 3.3387779953186936 7 6 3.1774360253900724 7 7 3.106995406173233 7 8 3.1909765471490985 7 9 3.317956975167654 7 10 3.381538952841993 7 11 4.403103423039852 7 12 6.899819319868129 7 13 6.616850058046214 7 14 6.965220058213674 7 15 6.952734549520855 7 16 7.0903668292601445 7 17 6.692568518655786 7 18 6.519838439478036 7 19 4.643522027010931 7 20 5.379157804549536 7 21 3.3351765509650306 7 22 3.484770832034016 7 23 3.4993334623794823 7 24 3.7402347305223245 7 25 3.317355923673354 7 26 2.635630788971035 7 27 2.3779001893650644 7 28 2.651410619186123 7 29 3.089753310053098 7 30 3.328211914159425 7 31 3.5847646120727603 7 32 3.3171017279102415 7 33 2.4805938993974763 7 34 2.930060692278275 7 35 1.7637447801677197 7 36 1.4100138907147297 7 37 1.7167492980740953 7 38 2.1225502385647594 7 39 4.9854243576988 7 40 -3.136097250957441 7 41 -8.466966806493263 7 42 4.528911192339141 7 43 8.824480920812402 7 44 7.79997767861364 7 45 4.444885749330968 7 46 7.733793857742377 7 47 8.134472630645664 7 48 7.615986313150856 7 49 10.432170991756387 7 50 7.248033339128089 7 51 6.905192407140724 7 52 6.802683412189467 7 53 7.7009951968548265 7 54 7.210098325981573 7 55 7.104881952948073 7 56 7.550103715730913 8 1 1.5646419779584733 8 2 2.2866463034277302 8 3 2.558511280624991 8 4 4.226784439956454 8 5 2.9603424773482914 8 6 3.3394339112251643 8 7 2.9938875837630903 8 8 3.447319041863686 8 9 2.961720188698308 8 10 2.657437472926553 8 11 4.791316953695244 8 12 6.834280207817393 8 13 7.538097415340079 8 14 7.913158747431439 8 15 6.653315944869689 8 16 7.354637442367963 8 17 6.838974438317518 8 18 6.4717411878924125 8 19 5.445583337793167 8 20 5.609337508566448 8 21 4.3933160858487526 8 22 4.747455821463191 8 23 3.652610417982018 8 24 4.909103652298228 8 25 4.023751272510566 8 26 3.0122164126496784 8 27 3.013206743276271 8 28 2.6194338750000767 8 29 2.3429245574103916 8 30 2.9674825272672436 8 31 3.1735595380769865 8 32 4.501034074633289 8 33 3.0923803687420843 8 34 3.577787965005548 8 35 3.061577761140235 8 36 1.4295699160847093 8 37 0.5164321733383659 8 38 1.4809011899389652 8 39 3.372845076514867 8 40 -2.548887948631858 8 41 -9.43076173249749 8 42 4.374048613057958 8 43 9.20582341552699 8 44 7.288878312863112 8 45 4.986376235588896 8 46 8.515770601928423 8 47 9.661956774408875 8 48 9.976715700043034 8 49 12.443798898733132 8 50 9.83180712348327 8 51 10.653606783462077 8 52 10.78814852846854 8 53 11.43461041884214 8 54 11.376060271013287 8 55 10.992831212990353 8 56 9.95681618930385 9 1 1.8088280244701025 9 2 2.2285067685440083 9 3 3.035255466671501 9 4 4.610505370189017 9 5 3.44871457037155 9 6 3.49059670192284 9 7 3.4706317698096 9 8 3.6566213674450836 9 9 3.7059062352099374 9 10 3.564414217112599 9 11 5.209921604858032 9 12 6.892419742701115 9 13 7.177632299061013 9 14 8.052693631152366 9 15 7.874246177427828 9 16 8.052311860972612 9 17 7.338974438317518 9 18 6.727555141380783 9 19 5.771164733142008 9 20 5.853523555078077 9 21 4.277037016081309 9 22 4.607920937742257 9 23 4.036331348214574 9 24 4.6416617918331085 9 25 4.105146621347771 9 26 3.4889605986961882 9 27 3.9201834874623174 9 28 3.7705966656977523 9 29 4.191761766712723 9 30 3.990738341220734 9 31 4.475885119472338 9 32 5.047545702540262 9 33 4.627264089672323 9 34 4.787090290586946 9 35 4.003438226256513 9 36 3.7784071253870373 9 37 3.516432173338366 9 38 3.8413663062180348 9 39 6.396100890468354 9 40 -1.397725157934186 9 41 -3.7447152208695833 9 42 5.5368393107323755 9 43 10.856986206224665 9 44 9.23073877797939 9 45 6.149166933263313 9 46 9.492514787974937 9 47 10.789863751153064 9 48 10.430204072136057 9 49 13.281008201058711 9 50 10.750411774646057 9 51 10.793141667183004 9 52 9.857915970329003 9 53 9.213680186284002 9 54 9.80629282915282 9 55 10.225389352525237 9 56 8.666118514885248 10 1 1.7099224157149706 10 2 2.4356203116356667 10 3 2.857006492663295 10 4 4.653733824361382 10 5 3.876211150398909 10 6 3.6115269344809775 10 7 3.3353376521625435 10 8 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-11.214704504423707 120 51 -11.846393216537924 120 52 -11.735107285484952 120 53 -11.879343069529952 120 54 -12.077428101079738 120 55 -11.73972692654453 120 56 -8.310625671161265 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 853.0 5 448.0 6 223.0 7 66.0 8 158.0 9 170.0 10 103.0 11 93.0 12 96.0 13 135.0 14 153.0 15 222.0 16 346.0 17 400.0 18 532.0 19 602.0 20 758.0 21 926.0 22 1144.0 23 1382.0 24 1510.0 25 1613.0 26 1695.0 27 1842.0 28 2022.0 29 2175.0 30 2445.0 31 2799.0 32 3360.0 33 4016.0 34 4823.0 35 5590.0 36 6001.0 37 5416.0 38 2697.0 39 817.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 100.0 0.0 0.0 0.0 7 99.49332824348008 0.3730631083965227 0.08849403966615191 0.04511460845725391 8 98.96930471447658 0.7756242300150961 0.12840311637833804 0.12666793912998212 9 92.27444718297636 4.682889575450411 1.6072482382754192 1.4354150032978095 10 35.8643785462685 41.691103746247684 10.398917249397027 12.045600458086794 11 29.648267365388953 32.43332350032102 18.792621770289266 19.12578736400076 12 34.27669136402284 17.827211049608717 19.64220645138901 28.253891134979437 13 25.248564140826986 16.799986118582012 23.242699241727543 34.70875049886346 14 22.835424584786796 19.767098800784435 24.59519966678815 32.80227694764062 15 25.45438748913988 20.842745438748914 23.62293657688966 30.079930495221546 16 26.21638980020483 22.7013140307938 24.36077696193303 26.72151920706834 17 27.61098191662906 27.09728922980806 20.01492485509007 25.276803998472808 18 27.919001926046743 28.980930402040567 20.324131109992884 22.7759365619198 19 25.020827547903362 31.57976950846987 18.753471257983893 24.64593168564288 20 24.911476775671733 30.734916336874264 20.202388391307366 24.151218496146637 21 23.848196158050914 26.751349193954223 25.19305186804796 24.2074027799469 22 25.095868542971665 27.45744477798407 24.035675244226198 23.411011434818064 23 26.12829900574344 29.433811663861466 24.878971386927173 19.558917943467925 24 24.209192969061792 28.446495809546946 23.214936405753846 24.129374815637416 25 24.583991254706667 29.324495497215043 23.692110149051725 22.399403099026564 26 22.119246752795117 26.156735233259727 30.11423901514493 21.60977899880023 27 21.551574488768534 22.45252230670416 31.590459327153418 24.405443877373884 28 20.258733111926414 26.16716802003095 31.67394063744327 21.900158230599367 29 20.45162634081994 25.60315201166383 34.18787100357552 19.757350643940708 30 18.267511500737783 25.315510806353615 38.35604548216301 18.060932210745595 31 18.170432604498252 24.355963749586877 40.412948564073126 17.060655081841745 32 21.928317278486507 20.086782955827477 39.293586739564354 18.69131302612167 33 20.085195166478307 23.171346605233417 41.302268973311314 15.441189254976962 34 17.53282845827833 22.83922740220941 45.633293962342805 13.994650177169458 35 17.91270227099104 25.10070143759983 44.11417459545802 12.872421695951108 36 16.61132455972601 24.956102988473774 43.10078058448219 15.331791867318023 37 17.862300558523135 24.444521775442382 43.09327858298679 14.599899083047694 38 18.47996106920644 25.878549827939796 43.14887552573951 12.492613577114255 39 17.012014626501827 21.05171513146439 38.547797318474665 23.388472923559114 40 11.046572475143904 16.19047619047619 30.903540903540904 41.859410430839 41 12.536744120940648 18.445548712206048 32.94197928331467 36.07572788353863 42 14.741271386841007 25.236472388371123 44.6115593267492 15.41069689803867 43 9.578263972290394 14.697230779941867 33.726698344733954 41.99780690303378 44 10.428506676894111 15.846727798891253 31.878246923050103 41.84651860116453 45 15.869927737632016 23.37409672040022 44.997220678154534 15.758754863813229 46 15.998124609293605 24.142182399110926 44.146349934014026 15.713343057581442 47 14.502187044365758 22.031868360758175 41.35076025827952 22.115184336596542 48 15.945425193980107 24.37639952090819 45.59183460917565 14.086340675936052 49 11.824306679897633 16.848592468794067 30.960463780226664 40.36663707108163 50 13.895315462626334 24.87344964948597 45.726859985735906 15.50437490215179 51 16.73284569836294 25.184604667363285 43.11389759665622 14.968652037617556 52 14.044777419635857 27.019775241745798 42.2876557191393 16.64779161947905 53 14.395770655444066 27.617689511851555 42.76298627897675 15.223553553727632 54 13.196929169416752 23.2865529579324 39.47441553479001 24.04210233786084 55 14.937434827945777 26.086200903719153 43.02572123740007 15.950643030935002 56 16.572491611175828 26.10010953283377 40.264617417459185 17.062781438531218 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 3.5 7 7.0 8 31.0 9 55.0 10 165.0 11 275.0 12 336.5 13 383.0 14 368.0 15 399.5 16 431.0 17 496.0 18 561.0 19 671.0 20 781.0 21 921.5 22 1273.5 23 1485.0 24 1776.5 25 2068.0 26 2380.5 27 2693.0 28 2912.0 29 3185.0 30 3239.0 31 3060.5 32 2882.0 33 2641.5 34 2401.0 35 2101.0 36 1801.0 37 1576.0 38 1228.0 39 1105.0 40 1072.0 41 1039.0 42 1027.0 43 1015.0 44 1063.5 45 1112.0 46 1237.0 47 1479.5 48 1597.0 49 1651.0 50 1705.0 51 1791.5 52 1878.0 53 1958.5 54 2115.5 55 2192.0 56 2349.5 57 2507.0 58 2676.5 59 2846.0 60 2792.5 61 2739.0 62 2655.5 63 2381.0 64 2190.0 65 1875.5 66 1561.0 67 1313.0 68 1065.0 69 933.5 70 802.0 71 656.5 72 451.0 73 391.0 74 300.0 75 209.0 76 164.5 77 120.0 78 85.0 79 37.0 80 24.0 81 16.5 82 9.0 83 5.0 84 1.0 85 1.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.02949801322205063 10 0.0 11 0.0034703544967118393 12 0.0 13 0.0 14 0.017351772483559194 15 0.14054935711682948 16 0.03817389946383023 17 0.015616595235203276 18 0.0 19 0.026027658725338795 20 0.03296836771876247 21 0.006940708993423679 22 0.0 23 0.0 24 0.0 25 0.0 26 0.20822126980271036 27 0.043379431208897985 28 0.20822126980271036 29 0.02949801322205063 30 0.04511460845725391 31 0.24639516926654056 32 0.027762835973694715 33 0.20128056080928666 34 0.10237545765299925 35 0.060731203692457186 36 0.19086949731915115 37 0.27415800524023526 38 0.1613714840971005 39 0.3487706269195398 40 0.5222883517551318 41 0.8311499019624855 42 0.2064860925543544 43 0.3088615502073537 44 0.4667626798077424 45 0.10931616664642294 46 0.07461262167930453 47 0.03296836771876247 48 0.03817389946383023 49 0.3296836771876247 50 0.2498655237632524 51 0.36612239940309904 52 0.409501830611997 53 0.22210268778955772 54 0.09890510315628742 55 0.15790112960038868 56 0.19781020631257484 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 57631.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.6987905814579 #Duplication Level Percentage of deduplicated Percentage of total 1 94.20556359794983 83.55919557182766 2 3.955553816659494 7.017056792351338 3 0.8803161313040417 2.3424892852804913 4 0.3795140654955202 1.3464975447241936 5 0.16432567784342111 0.7287744443094862 6 0.09781290347822685 0.5205531745067759 7 0.06455651629562972 0.4008259443702174 8 0.0528189678782425 0.37479828564487866 9 0.03325638718259713 0.2654821189984557 >10 0.15258812942603386 2.40669084346966 >50 0.01173754841738722 0.754802103034825 >100 0.001956258069564537 0.2828338914820149 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA 163 0.2828338914820149 No Hit AAAACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC 95 0.16484183859381235 No Hit AAAACGGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC 93 0.1613714840971005 No Hit AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA 71 0.12319758463327028 No Hit AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 71 0.12319758463327028 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0017351772483559195 0.0 0.0 0.0 0.0 10 0.003470354496711839 0.0 0.0 0.0 0.0 11 0.012146240738491437 0.0 0.0 0.0 0.0 12 0.015616595235203276 0.0 0.0 0.0 0.0 13 0.019086949731915114 0.0 0.0 0.0 0.0 14 0.02776283597369471 0.0 0.0 0.0 0.0 15 0.03470354496711839 0.0 0.0 0.0 0.0 16 0.03990907671218615 0.0 0.0 0.0 0.0 17 0.046849785705609825 0.0 0.0 0.0 0.0 18 0.050320140202321666 0.0 0.0 0.0 0.0 19 0.05552567194738942 0.0 0.0 0.0 0.0 20 0.07461262167930455 0.0 0.0 0.0 0.0 21 0.07634779892766046 0.0 0.0 0.0 0.0 22 0.08502368516944006 0.0 0.0 0.0 0.0 23 0.09543474865957557 0.0 0.0 0.0 0.0 24 0.10237545765299925 0.0 0.0 0.0 0.0 25 0.10758098939806701 0.0 0.0 0.0 0.0 26 0.10931616664642294 0.0 0.0 0.0 0.0 27 0.11278652114313477 0.0 0.0 0.0 0.0 28 0.11799205288820253 0.0 0.0 0.0 0.0 29 0.11799205288820253 0.0 0.0 0.0 0.0 30 0.12319758463327028 0.0 0.0 0.0 0.0 31 0.12666793912998212 0.0 0.0 0.0 0.0 32 0.12666793912998212 0.0 0.0 0.0 0.0 33 0.12666793912998212 0.0 0.0 0.0 0.0 34 0.12666793912998212 0.0 0.0 0.0 0.0 35 0.12666793912998212 0.0 0.0 0.0 0.0 36 0.12840311637833804 0.0 0.0 0.0 0.0 37 0.12840311637833804 0.0 0.0 0.0 0.0 38 0.12840311637833804 0.0 0.0 0.0 0.0 39 0.12840311637833804 0.0 0.0 0.0 0.0 40 0.13013829362669396 0.0 0.0 0.0 0.0 41 0.13013829362669396 0.0 0.0 0.0 0.0 42 0.13534382537176173 0.0 0.0 0.0 0.0 43 0.14054935711682948 0.0 0.0 0.0 0.0 44 0.14401971161354132 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACAAACC 45 1.09139364E-10 50.981304 38 TTAATTT 45 1.09139364E-10 50.981304 38 TAAACCA 15 0.007769172 50.9813 39 TTAGTTT 15 0.007769172 50.9813 38 TTATTTA 15 0.007934779 50.710365 37 TTTAGTT 15 0.007934779 50.710365 37 TTATTTC 25 1.8970106E-5 50.71036 37 CCGGACA 15 0.008074779 50.486774 36 AAGACCA 20 3.9563474E-4 50.486774 36 TAAGACC 20 3.9736225E-4 50.44229 35 TCCTTCA 15 0.00813129 50.39789 42 CCAACAC 45 1.2187229E-10 50.39789 43 CCTTCTA 15 0.00813129 50.39789 43 AGAATAT 15 0.008188095 50.30932 44 CTTCTAT 25 1.9884441E-5 50.30931 44 TTCAAGC 15 0.008245194 50.221054 34 GTAAGAC 15 0.008245194 50.221054 34 TAGAGCG 30 9.994146E-7 50.221054 34 AAGGAAC 15 0.008245194 50.221054 34 TGATACA 25 2.0092504E-5 50.22105 34 >>END_MODULE