FastQCFastQC Report
Fri 17 Jun 2016
SRR1526315_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526315_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76470
Sequences flagged as poor quality0
Sequence length56
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC1900.24846344971884401No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC1450.18961684320648622No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG1440.18830914083954492No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1290.16869360533542566No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG1280.16738590296848438No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1250.16346279586766052No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG1180.15430887929907153No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG900.11769321302471558No Hit
GCGAATGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC900.11769321302471558No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG860.11246240355695045No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC780.10200078462142016No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGTCG150.00798074250.65266439
TGCCCGT203.9656207E-450.48438637
TATGCCT150.00819333450.31721535
TTATTCC150.00819333450.31721535
CACGTCG150.00821482250.28391649
TCGCCTA309.99742E-750.2506642
ACAACAC150.00823635250.2506643
CCGATTG150.00823635250.2506644
ACCATAT150.00823635250.2506648
GCACGTC150.00823635250.2506648
CACGATT150.00823635250.2506647
CGATTGC150.00823635250.2506645
CACGACT150.00823635250.2506642
CCGTCAC150.00823635250.2506643
CCGAATC204.0575876E-450.2506643
ACCCGAT150.00823635250.2506642
CGTCACA150.00823635250.2506644
TCGAATC150.00823635250.2506643
ATCGAAT150.00823635250.2506642
CCCGATT150.00823635250.2506643