##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526315_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	76470
Sequences flagged as poor quality	0
Sequence length	56
%GC	54
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.12559173532104	39.0	37.0	39.0	33.0	40.0
2	36.20579312148555	39.0	36.0	39.0	29.0	40.0
3	36.5231724859422	38.0	36.0	39.0	30.0	40.0
4	34.569870537465675	38.0	35.0	39.0	18.0	40.0
5	35.206630051000396	39.0	35.0	39.0	22.0	40.0
6	36.20734928730221	38.0	36.0	39.0	29.0	40.0
7	36.69644304956192	38.0	36.0	39.0	31.0	40.0
8	36.35834967961292	38.0	36.0	39.0	30.0	40.0
9	36.30188309140839	38.0	36.0	39.0	29.0	40.0
10	36.17328364064339	38.0	36.0	39.0	29.0	40.0
11	35.346698051523475	38.0	34.0	39.0	29.0	40.0
12	33.080070615927816	37.0	31.0	39.0	19.0	40.0
13	32.56086046815745	36.0	30.0	39.0	17.0	40.0
14	32.126860206616975	36.0	29.0	39.0	15.0	40.0
15	32.64788806067739	37.0	30.0	39.0	17.0	40.0
16	32.491879168301296	36.0	30.0	38.0	18.0	40.0
17	32.503687720674776	36.0	30.0	38.0	18.0	40.0
18	32.678553681182166	36.0	30.0	38.0	20.0	40.0
19	33.41649012684713	36.0	31.0	38.0	24.0	40.0
20	33.63043023407872	36.0	31.0	38.0	24.0	40.0
21	35.014790113770104	38.0	35.0	39.0	27.0	40.0
22	34.67517980907545	38.0	33.0	39.0	26.0	40.0
23	34.913482411403166	38.0	34.0	39.0	27.0	40.0
24	34.53220870929776	38.0	33.0	39.0	25.0	40.0
25	34.780541388779916	38.0	34.0	39.0	27.0	40.0
26	35.02538250294233	38.0	35.0	39.0	27.0	40.0
27	34.975794429187914	38.0	35.0	39.0	27.0	40.0
28	34.84021184778344	38.0	35.0	39.0	27.0	40.0
29	34.68415064731267	38.0	34.0	39.0	27.0	40.0
30	34.50413233947953	38.0	34.0	39.0	26.0	40.0
31	34.18736759513535	38.0	33.0	39.0	25.0	40.0
32	33.15736890283772	38.0	33.0	39.0	18.0	40.0
33	34.469125147116515	38.0	34.0	39.0	26.0	40.0
34	34.103386949130375	38.0	33.0	39.0	25.0	40.0
35	34.11530011769322	38.0	33.0	39.0	25.0	40.0
36	32.20740159539689	35.0	31.0	38.0	22.0	39.0
37	31.96885052961946	35.0	31.0	38.0	20.0	39.0
38	31.28166601281548	35.0	30.0	38.0	15.0	39.0
39	29.258297371518243	33.0	27.0	38.0	6.0	39.0
40	25.371112854714266	31.0	12.0	37.0	4.0	39.0
41	22.17397672289787	26.0	9.0	33.0	4.0	37.0
42	25.58951222701713	30.0	20.0	34.0	4.0	37.0
43	21.764757421210934	26.0	8.0	33.0	4.0	36.0
44	22.831489472995948	27.0	10.0	33.0	4.0	37.0
45	25.191931476395972	30.0	19.0	34.0	4.0	37.0
46	25.102484634497188	30.0	15.0	36.0	4.0	38.0
47	24.45549888845299	30.0	10.0	35.0	4.0	38.0
48	24.41225317117824	30.0	10.0	35.0	4.0	38.0
49	21.85815352425788	26.0	5.0	35.0	4.0	38.0
50	24.163554335033346	29.0	4.0	35.0	4.0	38.0
51	25.690126847129594	32.0	4.0	38.0	4.0	39.0
52	25.495370733621026	32.0	4.0	38.0	4.0	39.0
53	25.135072577481367	32.0	4.0	37.0	4.0	39.0
54	24.26372433634105	31.0	4.0	37.0	4.0	39.0
55	24.39687459134301	31.0	4.0	37.0	4.0	39.0
56	23.686426049431148	30.0	4.0	37.0	4.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	1.3073269505695464
1	2	2.780993261754567
1	3	2.202031628352799
1	4	4.437895668017525
1	5	4.06015112848273
1	6	2.305830620802624
1	7	2.1116478531265983
1	8	2.431052202895472
1	9	2.539785703676266
1	10	2.228018270635644
1	11	-4.528530477604207
1	12	-9.189886560529704
1	13	-22.05823812736701
1	14	-19.100467131911778
1	15	-12.089177718431987
1	16	-21.948827033372474
1	17	-21.278402701900912
1	18	-21.486043268799435
1	19	-6.813418586313265
1	20	-11.43431051638235
1	21	-9.060482025304726
1	22	-3.026140764345037
1	23	-3.5132952620486755
1	24	-3.2896309338809395
1	25	-3.7689732947456633
1	26	2.4783603487981694
1	27	2.653303862766336
1	28	2.5013775093985657
1	29	2.6770928017888522
1	30	3.01397466318555
1	31	1.907270770963983
1	32	-11.988301681155242
1	33	1.635669982626439
1	34	1.7439245097613565
1	35	1.9549288068813837
1	36	5.099149200235519
1	37	4.851063759461173
1	38	5.845879423839815
1	39	8.120420365449785
1	40	10.9351620968418
1	41	-1.7657617211171157
1	42	-5.4594012468536945
1	43	-13.631983227727225
1	44	-16.050558698337785
1	45	-14.571738744351219
1	46	-18.336649228397317
1	47	-17.672040832576567
1	48	-16.79321227581587
1	49	-14.975138421324246
1	50	-16.61222523843923
1	51	-17.58254647029523
1	52	-17.547496624058745
1	53	-16.895143972095546
1	54	-17.726572684360917
1	55	-17.005037249012076
1	56	-16.079782160398928
2	1	1.101278266035493
2	2	2.505360116930376
2	3	2.014546995945622
2	4	3.8790070523302873
2	5	3.7918944282122595
2	6	1.8000524059021998
2	7	1.9287120281942194
2	8	2.1895965845035334
2	9	2.2779219220190186
2	10	1.6340915433182133
2	11	-3.947881350483481
2	12	-12.430604534466266
2	13	-14.115651454143503
2	14	-15.711113800217653
2	15	-11.69478381629282
2	16	-3.3449421059075135
2	17	-3.009654238660193
2	18	-10.659512656554536
2	19	-1.8528825646756921
2	20	-3.617861045027542
2	21	2.020290042558564
2	22	1.8157574407201196
2	23	1.5559203760137805
2	24	1.7225401996327037
2	25	1.9713758569632134
2	26	2.1525673810086445
2	27	2.287678094386699
2	28	1.852742151641003
2	29	2.242620217574448
2	30	2.561060967586833
2	31	-0.39024582603626357
2	32	-6.964082354870513
2	33	-0.6302508435353502
2	34	-0.5548214946645089
2	35	-0.5336377040603395
2	36	3.9066239973832886
2	37	3.7266477279883397
2	38	5.199088177220691
2	39	6.759220463802375
2	40	10.188788848747379
2	41	6.015157875637247
2	42	1.6856688293695647
2	43	-1.8768811869109001
2	44	-9.931306178052562
2	45	-1.330897829672093
2	46	-12.533723238724338
2	47	-12.709537759058003
2	48	-10.339315054276648
2	49	-12.135944912595455
2	50	-10.120216386705767
2	51	-9.771998154583404
2	52	-10.118433432517067
2	53	-10.342402393536414
2	54	-14.663381142684003
2	55	-10.1126103987539
2	56	-9.357751179331801
3	1	1.6293592658772837
3	2	2.81274818843805
3	3	2.3647289291840323
3	4	4.191141208318264
3	5	4.031571694467594
3	6	2.495986593530745
3	7	2.6653043395373572
3	8	2.7124568914169984
3	9	2.759174888321823
3	10	1.8996349699912045
3	11	0.8107802536930748
3	12	0.8777327539374653
3	13	-5.8488424049424275
3	14	-5.6295602485644345
3	15	-3.276811976394068
3	16	1.7221358880807784
3	17	2.11152911840356
3	18	0.9403133203478546
3	19	0.4341764449746819
3	20	0.04556246391091889
3	21	2.6114148645803965
3	22	2.6634081289023683
3	23	2.058293418253932
3	24	2.434848378718293
3	25	2.4679744964189965
3	26	2.1017842770693917
3	27	2.3095100829960913
3	28	1.6062621642972275
3	29	2.131561840735337
3	30	1.316954022483209
3	31	1.6611700891670296
3	32	0.39163667692825754
3	33	1.2673128330980958
3	34	1.8463808467371692
3	35	1.2575345788062933
3	36	3.53279074315137
3	37	4.343638710427829
3	38	4.986068085463057
3	39	7.106317442128596
3	40	9.28521346510555
3	41	7.76725406294938
3	42	5.4961418557894355
3	43	-0.019580126952032373
3	44	1.3099531163628804
3	45	5.1118327509322405
3	46	-1.6350521423788251
3	47	-6.188338581712646
3	48	-1.2029442268759212
3	49	-10.610236954993809
3	50	-1.393116244323231
3	51	-2.446654063458581
3	52	-1.8760667183895556
3	53	-1.8280850386874974
3	54	-5.257907325250052
3	55	-2.0489337653098225
3	56	1.1057039701698592
4	1	1.6500311005026091
4	2	3.150560419273532
4	3	2.3475024003295957
4	4	4.882786341823859
4	5	4.885318464493437
4	6	2.334746283453221
4	7	2.627578241346143
4	8	2.89798660717927
4	9	3.32523862647858
4	10	2.0958451336432304
4	11	3.0724512269919515
4	12	2.904089343084756
4	13	5.225920730285825
4	14	4.3025672277490905
4	15	5.739380960729104
4	16	6.71507301125046
4	17	5.9663094801606675
4	18	5.765808583052419
4	19	4.4472686069040535
4	20	4.003529733506092
4	21	3.3857473365872863
4	22	3.933520101339923
4	23	3.131506165885284
4	24	3.597294545815622
4	25	2.9124189408634393
4	26	2.1868833296103105
4	27	2.323808101945275
4	28	2.15251539427139
4	29	2.3644645108473057
4	30	2.7141977778665023
4	31	2.837569744636454
4	32	3.3625238431642615
4	33	2.4316539183694132
4	34	1.881006169734583
4	35	1.7333313057658088
4	36	3.219095652712088
4	37	3.482656798283127
4	38	4.857213649631877
4	39	6.7828032302422905
4	40	8.777375394476785
4	41	3.773283348048434
4	42	6.9698713992864185
4	43	6.553201957457961
4	44	10.602631841599745
4	45	7.690644120441284
4	46	7.642467237466139
4	47	7.757053321818791
4	48	7.275953275277395
4	49	3.6631480579260867
4	50	7.876306666960144
4	51	6.994517340503521
4	52	6.959419931050583
4	53	6.079063970787093
4	54	0.5455379805208338
4	55	6.173632987489487
4	56	5.370820249239628
5	1	2.293355096767783
5	2	3.7454561553457424
5	3	2.8734905733765927
5	4	4.936630101549973
5	5	4.871126120889329
5	6	3.038947964125491
5	7	3.1399965419997358
5	8	3.5017214344435104
5	9	3.610454935224304
5	10	2.5986773776519456
5	11	3.4644835954949116
5	12	6.16963556157215
5	13	6.379546625088192
5	14	6.033784158966018
5	15	5.804615943611143
5	16	6.718683344272556
5	17	5.6746194708236
5	18	5.547570176578716
5	19	4.325388743847718
5	20	4.702066926160931
5	21	2.8756321794133086
5	22	3.189106780456015
5	23	2.6285494276344252
5	24	3.396609827483843
5	25	3.084220995018434
5	26	1.9436838275872788
5	27	2.134016629800861
5	28	1.9067637602844627
5	29	1.9881184168541566
5	30	2.25493851860724
5	31	3.008313597733249
5	32	3.928474667938616
5	33	2.5000012573049872
5	34	1.8593441716019967
5	35	2.1254259213898408
5	36	3.7085447656906148
5	37	4.013562493894707
5	38	4.221297060774109
5	39	6.762821904378608
5	40	5.609557156070309
5	41	3.4365264230562147
5	42	7.793712046656481
5	43	10.88902517563412
5	44	11.52967821570494
5	45	8.953015735754075
5	46	9.562915416737845
5	47	10.531158519453403
5	48	9.81314593011711
5	49	9.95409110181031
5	50	9.708114945827248
5	51	8.537125494876381
5	52	9.350954322584975
5	53	10.106577198300318
5	54	9.169752110617317
5	55	10.34537279452341
5	56	9.730484114785845
6	1	1.8712871391499561
6	2	3.2178712083234515
6	3	2.5626392763360357
6	4	4.554605987556549
6	5	4.701743585266385
6	6	2.847789761714097
6	7	3.020814956321992
6	8	3.3289044815754067
6	9	3.3767684171388055
6	10	2.359291188393634
6	11	3.1664930949468655
6	12	5.538871951780408
6	13	5.9550672681280155
6	14	7.011423910518818
6	15	6.0672392538047895
6	16	6.158873333311654
6	17	5.897710446344242
6	18	5.687869774678823
6	19	4.01570661012466
6	20	4.415768058787897
6	21	2.693315774590509
6	22	3.224985479761827
6	23	2.9939860370608073
6	24	3.563800181886478
6	25	3.325945510911751
6	26	2.2570926693848676
6	27	2.502230002106309
6	28	2.608554041614383
6	29	2.7776690197039926
6	30	2.9679819968681116
6	31	2.4823362502725246
6	32	4.2076124099919525
6	33	2.541798845905646
6	34	2.8095085851935195
6	35	2.333975428149067
6	36	2.476100483629967
6	37	2.5106761219546208
6	38	3.982495453174547
6	39	5.111305645701226
6	40	1.8124800643640633
6	41	2.855086890721541
6	42	6.8681000627324735
6	43	10.828886337490164
6	44	10.499572260279294
6	45	7.010450883726307
6	46	9.970003469350196
6	47	10.358341566585299
6	48	10.088191600559195
6	49	11.400346125558936
6	50	10.445710853755639
6	51	10.150717018442329
6	52	9.661513328796154
6	53	10.405956080287897
6	54	11.622510605319547
6	55	10.193600781370325
6	56	9.814298510109193
7	1	2.0438803068518183
7	2	3.1545948108079287
7	3	2.524751077578273
7	4	4.169823378860897
7	5	4.7587311629061375
7	6	2.7133177120246543
7	7	2.4950385588064634
7	8	2.8630659722586387
7	9	3.195013758753717
7	10	2.195316033114132
7	11	2.701353343394068
7	12	4.76837505737047
7	13	6.154756709121802
7	14	6.765927016108879
7	15	5.932767204115347
7	16	6.509649730827178
7	17	6.001825353176542
7	18	5.210385302629135
7	19	4.737678659814101
7	20	4.254587934564292
7	21	3.3672288180687673
7	22	3.496879889699713
7	23	2.693675478054594
7	24	2.955958566979646
7	25	2.7328554487999455
7	26	2.147465340192319
7	27	2.155025033162204
7	28	2.301721743477735
7	29	2.0206814420642374
7	30	2.2638670900358093
7	31	2.6212337658004756
7	32	4.242550298190714
7	33	2.101892013607511
7	34	2.783188709417125
7	35	1.4421276020621079
7	36	0.5163178749343125
7	37	0.5429742586005872
7	38	1.6225575649757893
7	39	3.2171286270676838
7	40	-2.6976907430893533
7	41	-9.739944165179079
7	42	3.9229137273287478
7	43	8.323373915129917
7	44	7.430938719906621
7	45	5.489255231552395
7	46	8.046633904132804
7	47	8.633574485839958
7	48	8.539511476335598
7	49	10.151044883322914
7	50	8.404018307171786
7	51	8.771369192355372
7	52	9.975954322584975
7	53	9.40122005544318
7	54	10.456516816499672
7	55	9.173103886960387
7	56	8.856534534953912
8	1	1.892721158982141
8	2	2.887207592762813
8	3	2.529763608906592
8	4	4.297016361317034
8	5	4.205786300750745
8	6	2.2189568097690113
8	7	2.8427829197087178
8	8	2.492138653962897
8	9	3.2236791722875466
8	10	2.200328564442451
8	11	2.9878508371284056
8	12	4.970756009751419
8	13	5.578942172780952
8	14	6.387167617612636
8	15	5.341915073789533
8	16	6.054449229574047
8	17	5.747596029868269
8	18	5.005184801376004
8	19	4.535767632245182
8	20	4.538422521030462
8	21	2.6303867128056098
8	22	3.0040854034841757
8	23	1.837159187327778
8	24	2.782869594548565
8	25	2.406570987647065
8	26	1.4375655908188847
8	27	1.9733207725105757
8	28	1.1864335229263574
8	29	0.9508192866757668
8	30	1.0707279922914523
8	31	1.577530758281675
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115	19	-2.711927877716164
115	20	-3.261755891101565
115	21	0.3079067841704628
115	22	0.5770857008377277
115	23	0.49936555069382393
115	24	1.3695481553573714
115	25	0.710912349526339
115	26	1.2255525072625346
115	27	1.4080516675447754
115	28	0.9506321551000099
115	29	1.3097250255993487
115	30	0.11934529826825724
115	31	-0.5901766458217992
115	32	-6.723854060162797
115	33	-0.2779506014045978
115	34	-0.12313695644244405
115	35	-1.472067313192131
115	36	0.7430127901885477
115	37	-0.36743736367544244
115	38	2.120741584346252
115	39	3.5447617989805167
115	40	5.779760830760523
115	41	5.165624842084842
115	42	1.799124381081775
115	43	-3.1404578039984123
115	44	-0.7403687861466466
115	45	-0.9779057853967572
115	46	-5.873916943324822
115	47	-9.826625272029293
115	48	-7.7723831967878905
115	49	-8.998622186895005
115	50	-4.506241983385117
115	51	-1.879054536458188
115	52	-2.196261173783064
115	53	-2.6149724385035533
115	54	-1.8131563069870111
115	55	-1.871690301901598
115	56	0.7064134695786102
116	1	0.8307939529072499
116	2	2.2120308449230635
116	3	1.5044952140388261
116	4	3.143703975875823
116	5	2.4647546170639174
116	6	1.2362388207666584
116	7	1.5552731003842837
116	8	1.6605073368641783
116	9	1.5752109868987034
116	10	1.0780452442015545
116	11	-1.2742596630025176
116	12	-3.0093434927361393
116	13	-7.898015145888856
116	14	-10.89798556384848
116	15	-6.230878851321755
116	16	-10.026331079407363
116	17	-9.914619209723245
116	18	-4.530819388202421
116	19	-4.495130508629394
116	20	-3.9698257114911435
116	21	1.2403631464269793
116	22	0.8105792500408597
116	23	1.290157354387219
116	24	1.2018274369156785
116	25	0.9256859392050671
116	26	1.0264632079961586
116	27	1.0217627730342755
116	28	0.7302931720491657
116	29	1.3967475401452631
116	30	-0.5361062575121665
116	31	-1.369837662770955
116	32	-9.365392132512913
116	33	-1.0449629970534744
116	34	-0.8198230176617685
116	35	-1.3106709054005776
116	36	1.5069895167253584
116	37	1.4790083737391804
116	38	1.9674402941868792
116	39	4.747645684636982
116	40	7.301642945610006
116	41	4.223808500066124
116	42	0.8863998680536938
116	43	-11.273774272503346
116	44	-0.8734322822255756
116	45	0.35399403752254344
116	46	-7.189373558553763
116	47	-9.056324234842346
116	48	-8.542178289122827
116	49	-6.073502024992653
116	50	-5.758961436964675
116	51	-3.905869613614776
116	52	-3.4507514343446637
116	53	-4.332655211081342
116	54	-5.384368044907578
116	55	-4.220286304937268
116	56	-1.5038066164320156
117	1	-0.004623554152047404
117	2	1.8324423011554174
117	3	0.9591140119412032
117	4	3.0549585139960342
117	5	2.457090236265209
117	6	0.7779895266964658
117	7	0.7759265896944925
117	8	1.206695315887977
117	9	1.0992126004525602
117	10	0.26481410261219906
117	11	-9.035857081316355
117	12	-11.533748494753084
117	13	-6.545050240685146
117	14	-8.715250590068727
117	15	-8.056615035266894
117	16	-13.639481543304093
117	17	-13.286543372692186
117	18	-5.242800025556193
117	19	-4.811790452155009
117	20	-4.026300096323737
117	21	-0.3489873981474503
117	22	-0.02483825701843756
117	23	-0.5872125528334351
117	24	-3.572275023753946
117	25	0.1616681901126853
117	26	0.6561525988795793
117	27	1.284368662505834
117	28	0.24380668556268148
117	29	1.0115115578943588
117	30	-1.74506148139276
117	31	-1.964432257365548
117	32	-13.853492172851759
117	33	-2.0800577933422986
117	34	-2.5592340705056564
117	35	-2.1541561817216746
117	36	-0.7911145574981191
117	37	0.5931673087936353
117	38	-0.5077513153330884
117	39	2.84526569270475
117	40	1.4714694885708788
117	41	2.321831896596983
117	42	-0.5746731452581244
117	43	-6.740091335835334
117	44	-2.253424214456313
117	45	-2.3874339576367944
117	46	-7.107082312083412
117	47	-10.899002734237264
117	48	-9.87820087887676
117	49	-7.46357866880064
117	50	-6.99776740711393
117	51	-4.921198375212196
117	52	-4.888427916797266
117	53	-4.696511604788483
117	54	-7.1984059634231095
117	55	-5.662803448946949
117	56	-2.483233804814425
118	1	0.9732634237349345
118	2	2.258977927691042
118	3	1.6195562723834698
118	4	2.465277924315444
118	5	3.0954194745944434
118	6	1.3892917379986756
118	7	1.5164671302350357
118	8	2.011117920310582
118	9	2.0289423301822893
118	10	1.2810303188284138
118	11	-6.941016786476059
118	12	-10.786819747824335
118	13	-10.984853680488584
118	14	-1.0233586981768354
118	15	-17.445804224456083
118	16	-7.8458697496923016
118	17	-12.625609711758525
118	18	-3.558770541526709
118	19	-3.5257953661599224
118	20	-4.2032042732279145
118	21	0.5035924544324004
118	22	0.18941235723217886
118	23	0.3267923611714778
118	24	-1.7010219525008736
118	25	-0.2289952005507061
118	26	1.483179625906601
118	27	1.4524276305648058
118	28	-0.12425228249628617
118	29	0.9476294940122898
118	30	1.07312033678906
118	31	-0.2243831173164068
118	32	-7.696735416095002
118	33	-0.9380430513275542
118	34	-0.644246355517943
118	35	-0.6249178524833496
118	36	1.4594996931161326
118	37	1.8079093235356503
118	38	2.5777523701705967
118	39	4.548459795898854
118	40	6.961887178988569
118	41	3.9847311594962456
118	42	1.2459656753806954
118	43	-2.231983227727227
118	44	-2.8775028385349373
118	45	-0.7255174957203323
118	46	-6.563593368594468
118	47	-8.013990449224979
118	48	-8.421689822365703
118	49	-7.062104467326437
118	50	-5.834131043477566
118	51	-5.971812625826445
118	52	-5.913980742350091
118	53	-7.750565658842536
118	54	-10.665236430253577
118	55	-9.02300000914351
118	56	-6.647361568942191
119	1	0.8359721435865097
119	2	2.2196458312160914
119	3	1.5349551592109236
119	4	3.205537664575182
119	5	3.138833203722463
119	6	0.5959707732491424
119	7	1.4185079465615615
119	8	1.7814333191974114
119	9	1.8697586567128965
119	10	1.2973568494406607
119	11	-9.631554819871234
119	12	-9.226522901813205
119	13	-2.9625902296537063
119	14	-3.3539709430747955
119	15	-7.878457285680572
119	16	-10.222493126315676
119	17	-11.315776687639787
119	18	-3.1595126565545364
119	19	-3.3732907279410007
119	20	-3.301534514415298
119	21	-0.7450160798904193
119	22	-1.1026099062186532
119	23	-0.8216306443943822
119	24	-7.961133269755049
119	25	-1.5796445512000545
119	26	1.4995061565188479
119	27	0.6754331964275124
119	28	0.13845643735528768
119	29	0.5283345032887325
119	30	0.35697933493376865
119	31	-2.8188172546076906
119	32	-12.15795990589092
119	33	-2.028210027208818
119	34	-2.554821494664509
119	35	-0.9826172958970787
119	36	1.7637668545261498
119	37	1.6450150749271195
119	38	3.7399045037513012
119	39	4.902077606659521
119	40	7.198992930380033
119	41	6.709035426657657
119	42	2.7468933191654834
119	43	-0.4483097583394695
119	44	-2.0945714841750096
119	45	-3.0757957888557677
119	46	-9.890866095867196
119	47	-12.576884697833513
119	48	-12.176049748154199
119	49	-9.380842871779128
119	50	-10.569195978542501
119	51	-11.353630807644628
119	52	-12.404147718231354
119	53	-11.485259536393556
119	54	-12.296034203908492
119	55	-14.102406317121247
119	56	-11.02101648545425
120	1	0.6478088782803866
120	2	2.173523382236496
120	3	1.430465363292555
120	4	2.6198233788609
120	5	3.1608740200489933
120	6	1.1947462834532203
120	7	1.396467130235031
120	8	1.52020882940149
120	9	1.6689423301822899
120	10	1.0210303188284158
120	11	-10.848289513748785
120	12	-2.2759106569152436
120	13	-8.506671862306767
120	14	-7.503358698176836
120	15	-8.825804224456082
120	16	-9.556778840601392
120	17	-7.889246075394887
120	18	-3.426043268799436
120	19	-3.4712499116144677
120	20	-4.546840636864278
120	21	-0.05277118193123442
120	22	0.24759417541400097
120	23	-0.46775309337397886
120	24	-6.110112861591784
120	25	-0.6853588369143395
120	26	1.3231796259066044
120	27	1.4978821760193526
120	28	0.7902931720491679
120	29	1.5785385849213824
120	30	1.9149385186072365
120	31	-1.9098376627709541
120	32	-8.336735416095
120	33	-1.7216794149639192
120	34	-10.467882719154307
120	35	-2.3903723979378917
120	36	-1.218682125065687
120	37	-0.228454312827985
120	38	-0.9131567207384954
120	39	2.053914341353398
120	40	0.24552354262493026
120	41	-0.6970870223219379
120	42	-4.0649434155283934
120	43	-4.071983227727225
120	44	-7.024775565807664
120	45	-8.278244768447605
120	46	-11.810866095867196
120	47	-10.870354085588614
120	48	-9.814417095092976
120	49	-14.445740830962801
120	50	-9.697767407113929
120	51	-9.893630807644627
120	52	-9.697617105986454
120	53	-9.850565658842536
120	54	-10.64705461207176
120	55	-10.441181827325329
120	56	-6.269179750760372
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	707.0
5	33.0
6	40.0
7	93.0
8	176.0
9	204.0
10	118.0
11	106.0
12	129.0
13	147.0
14	226.0
15	273.0
16	431.0
17	558.0
18	711.0
19	857.0
20	1070.0
21	1348.0
22	1652.0
23	1902.0
24	2109.0
25	2235.0
26	2492.0
27	2571.0
28	2712.0
29	2705.0
30	3074.0
31	3801.0
32	4514.0
33	5308.0
34	6431.0
35	7583.0
36	8328.0
37	7210.0
38	3678.0
39	938.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.54230417157055	0.4119262455865045	0.026154047338825683	0.019615535504119264
8	99.70838237217208	0.1647704982346018	0.06930822544788806	0.0575389041454165
9	99.13932925234134	0.3832470046565165	0.1674253126144509	0.30999843038769426
10	56.154047338825684	29.37884137570289	6.993592258401987	7.473519027069439
11	36.95842651078243	28.73919468528908	17.688676160958323	16.61370264297017
12	36.69805152347326	13.06525434811037	21.523473257486597	28.713220870929778
13	26.877206747744214	12.01647704982346	25.161501242317247	35.94481496011508
14	22.456186241171856	14.506931729008631	29.55532304472927	33.481558985090246
15	27.775375705881583	15.755407937319028	27.407203595246514	29.062012761552875
16	34.08522942862003	16.441403132318886	24.3415457483416	25.13182169071949
17	30.726973081853142	20.210845737306094	23.963428989980905	25.098752190859862
18	30.09311327910444	22.22164099076714	24.12444746684801	23.56079826328041
19	30.708259760643514	24.692956641161466	20.49310051664378	24.105683081551238
20	28.505644876440655	26.551196347509844	21.306628641697518	23.636530134351986
21	27.97619826064212	23.230236055711764	25.58948538547048	23.204080298175636
22	28.11858400136003	21.5119852489244	25.517529979468804	24.851900770246765
23	28.721067085131423	21.532627174055186	25.32496403818491	24.42134170262848
24	28.53275794429188	23.16463972799791	23.92964561265856	24.372956715051654
25	30.531725689177176	22.592456975466863	21.49657373018779	25.379243605168174
26	25.323220811883523	22.043200901219528	24.842483069386045	27.791095217510904
27	24.56149529776464	18.368147750905788	26.44500542817156	30.625351523158017
28	24.578546821581547	24.096511795449484	25.141794270594552	26.183147112374417
29	28.134524572579693	23.206927675382946	25.616440148076446	23.042107603960915
30	27.107425043168853	20.709277379519648	29.697556381141748	22.485741196169744
31	26.918741808650065	22.59370904325033	29.062909567496725	21.424639580602882
32	31.24975475770061	18.711660453861747	27.171538813681252	22.86704597475639
33	29.79264091379468	19.70107805766233	28.740781493561784	21.765499534981203
34	24.008429981412153	23.021441474461348	34.4686755504359	18.501452993690602
35	23.722670713995996	25.80957489958	33.21252404192126	17.255230344502742
36	23.714229585488837	28.30462969026259	29.073407111518563	18.90773361273001
37	24.438706633288334	26.042963681402938	30.420593208120895	19.097736477187834
38	28.18467583497053	25.388343156516047	27.87426326129666	18.552717747216764
39	23.445623063592922	20.17145407761382	25.492306884419474	30.890615974373787
40	15.91886665877432	14.913907633216263	22.04859055812057	47.118635149888846
41	16.255605381165918	17.49142706409918	24.690055394355053	41.562912160379845
42	22.285272455639138	23.40707152779598	31.34484837365345	22.962807642911436
43	13.416122976751513	13.221838612704623	26.060359426073486	47.30167898447038
44	15.473246698584731	14.260535592150262	23.092807912874207	47.1734097963908
45	23.007528641571195	21.4468085106383	32.02487725040917	23.52078559738134
46	20.733527055682085	23.63019974344878	30.783528364616874	24.852744836252256
47	18.825068690304857	21.615857647520606	30.474944393562737	29.084129268611804
48	22.063037249283667	24.873415237272834	33.37258442255106	19.69096309089244
49	16.92873080456753	15.637222732642078	22.602703766898543	44.83134269589185
50	21.179199538699446	22.429428878463032	33.081278012213986	23.31009357062354
51	21.20806601855132	25.052150982012833	32.555332519909214	21.18445047952664
52	20.598813399138926	24.035230494592042	32.45825895201092	22.90769715425811
53	21.368305209097578	22.952258673095063	34.95571743003878	20.723718687768578
54	18.263967102333744	21.32062128395191	29.78600801487729	30.629403598837058
55	21.801723912075243	24.387592024941707	31.342188687154497	22.46849537582855
56	22.585886114095544	23.374649511281152	30.011006000890962	24.028458373732345
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	1.0
11	2.0
12	17.5
13	62.0
14	91.0
15	124.0
16	157.0
17	168.0
18	179.0
19	227.5
20	276.0
21	347.5
22	440.5
23	462.0
24	564.0
25	666.0
26	839.5
27	1013.0
28	1166.0
29	1478.0
30	1637.0
31	1758.5
32	1880.0
33	1931.0
34	1982.0
35	1893.0
36	1804.0
37	1646.0
38	1412.0
39	1336.0
40	1325.0
41	1314.0
42	1350.0
43	1386.0
44	1387.5
45	1389.0
46	1575.5
47	2100.0
48	2438.0
49	2488.0
50	2538.0
51	2615.5
52	2693.0
53	2905.0
54	3377.0
55	3637.0
56	3860.5
57	4084.0
58	4507.5
59	4931.0
60	5446.5
61	5962.0
62	6092.0
63	5776.5
64	5331.0
65	4821.5
66	4312.0
67	3773.0
68	3234.0
69	2867.5
70	2501.0
71	2110.5
72	1458.0
73	1196.0
74	1043.0
75	890.0
76	717.0
77	544.0
78	414.5
79	210.0
80	135.0
81	107.0
82	79.0
83	47.5
84	16.0
85	11.5
86	7.0
87	4.5
88	1.5
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.023538642604943115
10	0.0
11	0.003923107100823852
12	0.0
13	0.0
14	0.013077023669412841
15	0.19223224794036878
16	0.053615797044592656
17	0.020923237871060547
18	0.005230809467765137
19	0.019615535504119264
20	0.037923368641297237
21	0.006538511834706421
22	0.0013077023669412842
23	0.0
24	0.0
25	0.0026154047338825684
26	0.16869360533542566
27	0.02223094023800183
28	0.1660782006015431
29	0.030077154439649534
30	0.03400026154047339
31	0.22230940238001834
32	0.019615535504119264
33	0.16869360533542566
34	0.09938537988753761
35	0.053615797044592656
36	0.15038577219824767
37	0.22623250948084214
38	0.1569242840329541
39	0.38969530534850266
40	0.584542958022754
41	0.8500065385118346
42	0.21446318817837062
43	0.3831567935137962
44	0.5780044461880476
45	0.12423172485942201
46	0.09415457041977246
47	0.052308094677651365
48	0.05100039231071008
49	0.36615666274355957
50	0.21446318817837062
51	0.32561788936837976
52	0.3740028769452073
53	0.18569373610566237
54	0.1464626650974238
55	0.17261671243624951
56	0.19353995030731005
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	76470.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.5054269648228
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.15449790475026	79.65215117039361
2	4.4459058514055	7.602981561396627
3	1.1455051540085033	2.9384072185170655
4	0.4450493989539045	1.5221655551196547
5	0.22634814792157343	0.9676997515365503
6	0.12387972960572599	0.6355433503334641
7	0.09788028018230202	0.5858506603896952
8	0.05505765760254489	0.37661828167908984
9	0.05046951946899948	0.3883876029815614
>10	0.23093628605511882	3.1947168824375574
>50	0.013764414400636223	0.8552373479795998
>100	0.010705655644939284	1.2802406172355172
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC	190	0.24846344971884401	No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC	145	0.18961684320648622	No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG	144	0.18830914083954492	No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	129	0.16869360533542566	No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG	128	0.16738590296848438	No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	125	0.16346279586766052	No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	118	0.15430887929907153	No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG	90	0.11769321302471558	No Hit
GCGAATGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC	90	0.11769321302471558	No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG	86	0.11246240355695045	No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	78	0.10200078462142016	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0026154047338825684	0.0	0.0	0.0	0.0
11	0.006538511834706421	0.0	0.0	0.0	0.0
12	0.006538511834706421	0.0	0.0	0.0	0.0
13	0.006538511834706421	0.0	0.0	0.0	0.0
14	0.010461618935530274	0.0	0.0	0.0	0.0
15	0.010461618935530274	0.0	0.0	0.0	0.0
16	0.01569242840329541	0.0	0.0	0.0	0.0
17	0.01569242840329541	0.0	0.0	0.0	0.0
18	0.01569242840329541	0.0	0.0	0.0	0.0
19	0.01830783313717798	0.0	0.0	0.0	0.0
20	0.019615535504119264	0.0	0.0	0.0	0.0
21	0.019615535504119264	0.0	0.0	0.0	0.0
22	0.019615535504119264	0.0	0.0	0.0	0.0
23	0.023538642604943115	0.0	0.0	0.0	0.0
24	0.026154047338825683	0.0	0.0	0.0	0.0
25	0.030077154439649537	0.0	0.0	0.0	0.0
26	0.035307963907414676	0.0	0.0	0.0	0.0
27	0.04315417810906238	0.0	0.0	0.0	0.0
28	0.045769582842944946	0.0	0.0	0.0	0.0
29	0.04707728520988623	0.0	0.0	0.0	0.0
30	0.0496926899437688	0.0	0.0	0.0	0.0
31	0.0496926899437688	0.0	0.0	0.0	0.0
32	0.0496926899437688	0.0	0.0	0.0	0.0
33	0.0496926899437688	0.0	0.0	0.0	0.0
34	0.0496926899437688	0.0	0.0	0.0	0.0
35	0.0496926899437688	0.0	0.0	0.0	0.0
36	0.0496926899437688	0.0	0.0	0.0	0.0
37	0.0496926899437688	0.0	0.0	0.0	0.0
38	0.0496926899437688	0.0	0.0	0.0	0.0
39	0.05100039231071008	0.0	0.0	0.0	0.0
40	0.05100039231071008	0.0	0.0	0.0	0.0
41	0.052308094677651365	0.0	0.0	0.0	0.0013077023669412842
42	0.052308094677651365	0.0	0.0	0.0	0.0013077023669412842
43	0.05492349941153393	0.0	0.0	0.0	0.0013077023669412842
44	0.05492349941153393	0.0	0.0	0.0	0.0013077023669412842
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTGTCG	15	0.007980742	50.652664	39
TGCCCGT	20	3.9656207E-4	50.484386	37
TATGCCT	15	0.008193334	50.317215	35
TTATTCC	15	0.008193334	50.317215	35
CACGTCG	15	0.008214822	50.283916	49
TCGCCTA	30	9.99742E-7	50.25066	42
ACAACAC	15	0.008236352	50.25066	43
CCGATTG	15	0.008236352	50.25066	44
ACCATAT	15	0.008236352	50.25066	48
GCACGTC	15	0.008236352	50.25066	48
CACGATT	15	0.008236352	50.25066	47
CGATTGC	15	0.008236352	50.25066	45
CACGACT	15	0.008236352	50.25066	42
CCGTCAC	15	0.008236352	50.25066	43
CCGAATC	20	4.0575876E-4	50.25066	43
ACCCGAT	15	0.008236352	50.25066	42
CGTCACA	15	0.008236352	50.25066	44
TCGAATC	15	0.008236352	50.25066	43
ATCGAAT	15	0.008236352	50.25066	42
CCCGATT	15	0.008236352	50.25066	43
>>END_MODULE