FastQCFastQC Report
Fri 17 Jun 2016
SRR1526314_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526314_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9788
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC300.3064977523498161No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTCTTTTTTTTTTTTTTT270.2758479771148345No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC260.2656313853698406No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT230.234981610134859No Hit
GTCCCGGGGACACGCAGGGATGAGATGGCCAAAAAAAAACCAAAAAAAAAAAAAAA230.234981610134859No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTCCTTTTTTTTTTTTTTT210.21454842664487125No Hit
GTCCCGGGGACACGCAGGGATGAGATGGGGAAAAAAAAACCAAAAAAAAAAAAAAA160.1634654679199019No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC140.14303228442991417No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTCTTTTTTTTTTTTTTTT140.14303228442991417No Hit
GTCCCGGGGACACGCAGGGATGAGATGGGCAAAAAAAAACCAAAAAAAAAAAAAAA110.11238250919493258No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTCCCTACCAAGCGC110.11238250919493258No Hit
GTCCCGGGGGTCCAAAATAAATCCCGATTTCCTTCTCATCTACACATACTTAACCA100.1021659174499387No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGCT150.00806383550.18556636
ACCAACA150.00806383550.18556642
AGAGCGC150.00806383550.18556635
CGGGGCA402.2719178E-949.9282075
CCGGGCA204.0099074E-449.9282074
GGGGAAC204.0099074E-449.9282076
ACAAAAC150.00823068549.92820448
GGGCAGC150.00823068549.9282047
CGGGGGG1700.049.9282045
CGGGGAT600.049.9282045
GGACCCG150.00823068549.9282048
GCAGGGA600.049.92820414
GGGAGTC150.00823068549.9282047
GGGAGGC150.00823068549.9282047
AGATGGG150.00823068549.92820423
GAACAGT150.00823068549.9282049
CCGGGAG150.00823068549.9282044
GGGAACA150.00823068549.9282047
GGAACAG150.00823068549.9282048
AAGCAGC150.00823068549.92820428
Produced by FastQC (version 0.11.5)