FastQCFastQC Report
Fri 17 Jun 2016
SRR1526313_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526313_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98081
Sequences flagged as poor quality0
Sequence length56
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG7990.8146328034991487No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG6500.6627175497802836No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG6400.6525218951682793No Hit
TATGTAGGGATGAGATGGACTTAAATGAATTGACGGGTACCCACAGTTCTGAGTCG6040.6158175385650636No Hit
TATGTAGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG5810.5923675329574535No Hit
TATGTAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG5790.5903284020350527No Hit
TATGTAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG5630.5740153546558457No Hit
TATGTAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG5440.5546436108930374No Hit
TATGTAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG5270.53731099805263No Hit
TATGTAGGGATGAGATGGACTCAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG5250.5352718671302291No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG4870.4965283796046125No Hit
TATGTAGGGATGAGATGGACTCAAATGAATTGACGGGTACCCACAGTTCTGAGTCG4470.45574576115659504No Hit
TATGTAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG4060.4139435772473772No Hit
TATGTAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG3990.4068066190189741No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG3700.37723922064416143No Hit
TATGTAGGGATGAGATGGACTCAAATGAATTGACGGGTACGCACAGTTCTGAGTCG3530.35990660780375405No Hit
TATGTAGGGATGAGATGGACTCAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG3320.3384957331185449No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAACGATTAAG2700.2752826745241178No Hit
TATGTAGGGATGCGCTGGACTTAAAGGAATTGACGGGTCCGCCCAGTTCTGAGTCG2230.22736309784769732No Hit
TATGTAGGGATGCGCTGGACTTAAATGAATTGACGGGTCCGCCCAGTTCTGAGTCG2140.2181870086968934No Hit
TATGTAGGGATGCGCTGGACTCAAAGGAATTGACGGGTCCGCCCAGTTCTGAGTCG1810.184541348477279No Hit
TATGTAGGGATGCGCTGGACTCAAATGAATTGACGGGTCCGCCCAGTTCTGAGTCG1560.15905221194726807No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTCAG1490.15191525371886502No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGCCTCAAAGATTAAG1480.15089568825766458No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC1360.13866090272325934No Hit
TATGTAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC1360.13866090272325934No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTCAG1070.1090935043484467No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAAAGATTAAG1030.10501524250364495No Hit
TATGTAGGGATGCGCTGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG990.10093698065884321No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG990.10093698065884321No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACAACC150.00804853850.5554438
CAGCCCA150.00804853850.5554439
TAAACAC203.9437826E-450.5554438
CCGTCAT150.00804853850.5554439
TAACCAC402.39379E-950.5554438
TACGACC451.200533E-1050.5554438
ACACTAG150.00804853850.5554440
CGTCATC150.00804853850.5554440
TAGTCTC203.9437826E-450.5554440
CTTCAAT203.9741548E-450.4769841
CACTAGT150.0080981450.4769841
AAACACC150.0080981450.4769841
CAGTAGT203.9741548E-450.4769841
GTACGAC505.456968E-1250.4248137
TGCTCGT600.050.42480537
GTACGCG203.994506E-450.42480537
GTACACA354.8854417E-850.424837
GATACGC252.0023726E-550.29484636
CAAGAGA150.00821476250.2948436
TATGCTA204.0560565E-450.26893635
Produced by FastQC (version 0.11.5)