##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526313_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 98081 Sequences flagged as poor quality 0 Sequence length 56 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.08651012938286 38.0 37.0 39.0 33.0 40.0 2 35.34370571262528 38.0 35.0 39.0 27.0 40.0 3 35.721210020289355 38.0 35.0 39.0 27.0 40.0 4 36.032830007850656 38.0 35.0 39.0 29.0 40.0 5 35.698269797412344 38.0 35.0 39.0 27.0 40.0 6 33.64792365493827 38.0 33.0 39.0 14.0 40.0 7 36.31421987948736 38.0 35.0 39.0 30.0 40.0 8 36.185193870372444 38.0 35.0 39.0 30.0 40.0 9 36.17160306277464 38.0 35.0 39.0 30.0 40.0 10 35.63114160744691 38.0 35.0 39.0 29.0 40.0 11 34.59896412149142 38.0 33.0 39.0 26.0 40.0 12 32.65993413607121 37.0 30.0 39.0 18.0 40.0 13 32.03812155259428 36.0 29.0 39.0 16.0 40.0 14 31.056769404879642 36.0 26.0 39.0 12.0 40.0 15 31.869444642693285 36.0 28.0 39.0 16.0 40.0 16 32.349048235641966 36.0 29.0 39.0 17.0 40.0 17 32.77124009747046 36.0 30.0 39.0 19.0 40.0 18 32.95432346733822 36.0 30.0 39.0 20.0 40.0 19 33.60936368919567 36.0 31.0 39.0 24.0 40.0 20 33.83735891762931 37.0 31.0 39.0 24.0 40.0 21 35.496079770801686 38.0 35.0 39.0 28.0 40.0 22 35.22276485761768 38.0 35.0 39.0 27.0 40.0 23 35.223009553328374 38.0 35.0 39.0 27.0 40.0 24 34.8270715021258 38.0 34.0 39.0 25.0 40.0 25 35.124896768997054 38.0 35.0 39.0 27.0 40.0 26 35.45482815224152 38.0 35.0 39.0 28.0 40.0 27 35.55721291585526 38.0 35.0 39.0 29.0 40.0 28 34.9131941966334 38.0 35.0 39.0 27.0 40.0 29 35.11269257042648 38.0 35.0 39.0 27.0 40.0 30 34.74407887358408 38.0 35.0 39.0 26.0 40.0 31 34.761747943026684 38.0 35.0 39.0 26.0 40.0 32 33.50177914172979 38.0 33.0 39.0 18.0 40.0 33 34.75997389912419 38.0 35.0 39.0 27.0 40.0 34 34.66543978956169 38.0 35.0 39.0 26.0 40.0 35 34.8454236804274 38.0 35.0 39.0 27.0 40.0 36 32.920769568010115 36.0 32.0 38.0 23.0 39.0 37 32.73269032738247 36.0 32.0 38.0 23.0 39.0 38 31.357429063733036 35.0 30.0 38.0 16.0 39.0 39 30.10321061163732 35.0 28.0 38.0 7.0 39.0 40 26.26542347651431 32.0 13.0 38.0 4.0 39.0 41 22.096828131850206 25.0 10.0 33.0 4.0 37.0 42 25.898828519285082 30.0 21.0 34.0 4.0 37.0 43 22.428329645904917 27.0 9.0 33.0 4.0 37.0 44 23.751898940671484 29.0 11.0 34.0 4.0 37.0 45 25.90106136764511 30.0 22.0 34.0 4.0 37.0 46 26.240128057421927 31.0 19.0 36.0 4.0 38.0 47 25.356888694038602 30.0 15.0 36.0 4.0 38.0 48 25.527941191464198 31.0 16.0 36.0 4.0 38.0 49 23.25367808240128 29.0 6.0 35.0 4.0 38.0 50 25.444795628103304 31.0 16.0 36.0 4.0 38.0 51 26.6338536515737 33.0 15.0 38.0 4.0 40.0 52 26.58080566062744 33.0 11.0 38.0 4.0 40.0 53 26.28489717682324 33.0 4.0 38.0 4.0 40.0 54 25.311191770067598 32.0 4.0 38.0 4.0 39.0 55 25.47000948195879 32.0 4.0 38.0 4.0 39.0 56 25.327504817446805 33.0 4.0 38.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.6454580073625564 1 2 3.565458427186634 1 3 3.811773963636462 1 4 2.6664534955094155 1 5 2.725235497593573 1 6 5.99875791019435 1 7 2.382071619262696 1 8 2.4975003098408877 1 9 2.492427153757589 1 10 2.7608090112780843 1 11 -3.4792931675576355 1 12 -7.088168615656556 1 13 -20.16719451739047 1 14 -17.233931042895986 1 15 -12.486212642749763 1 16 -21.18212152254259 1 17 -21.67598530977567 1 18 -20.730701422291922 1 19 -6.934102368370066 1 20 -11.200658548315218 1 21 -8.739722900051106 1 22 -2.708967891481855 1 23 -3.6342094567888523 1 24 -2.961601132926276 1 25 -3.2173512616022215 1 26 2.038063589127269 1 27 1.9334431083846724 1 28 2.0470665906149677 1 29 1.9254534422567602 1 30 2.0089044856002047 1 31 0.5354820354248702 1 32 -12.558257811143893 1 33 -0.11501708679875833 1 34 0.15302920005950682 1 35 0.08699758461898455 1 36 3.0031029385501036 1 37 2.6925485254656856 1 38 2.6285064710511143 1 39 4.836189382027243 1 40 7.327877126673606 1 41 -2.3406230139631283 1 42 -7.322394803887374 1 43 -14.905772428846586 1 44 -17.205449171565178 1 45 -16.507195252631213 1 46 -18.93445054686956 1 47 -18.53195356724796 1 48 -16.719852411050653 1 49 -15.49648551547043 1 50 -16.76237746609482 1 51 -17.5633993871472 1 52 -17.569754838074857 1 53 -17.154281034603635 1 54 -18.727243347467294 1 55 -17.594970585629234 1 56 -17.28453945178237 2 1 1.7614624439776776 2 2 3.6254832200358535 2 3 4.012596042321135 2 4 2.5781126590769787 2 5 2.936300937666651 2 6 6.13968568470991 2 7 2.6451367983584078 2 8 2.6564355222112184 2 9 2.800967637870592 2 10 2.9682207683081643 2 11 -2.3773815083940697 2 12 -10.792089800493773 2 13 -13.115521000068096 2 14 -15.323707254809602 2 15 -6.397806561977273 2 16 -3.691679818360427 2 17 -2.0108192629302337 2 18 -9.757647334734017 2 19 -1.9318840608082475 2 20 -3.1230177836515516 2 21 2.285526659549596 2 22 2.420281146164129 2 23 2.5583265907090222 2 24 2.71367778662745 2 25 2.2845734464293557 2 26 2.729523105014266 2 27 2.3406330346585733 2 28 1.9185352407095664 2 29 2.9758220727780653 2 30 2.7215031676645367 2 31 -0.5985298390450211 2 32 -9.902813170966429 2 33 -0.6742263253973135 2 34 -0.6339480296658095 2 35 -1.1316361989002885 2 36 3.979810709151007 2 37 4.408931379253122 2 38 5.729113243413611 2 39 7.240182335638515 2 40 9.799854682620627 2 41 6.541023753179822 2 42 2.0374929758477336 2 43 -1.0122511918138954 2 44 -11.026027236418054 2 45 -0.7995551404109449 2 46 -10.481328605198 2 47 -11.394679475775394 2 48 -7.817718921130901 2 49 -11.439853428303993 2 50 -7.583020775287748 2 51 -7.032179970431603 2 52 -7.627561323362258 2 53 -7.924425224595154 2 54 -15.327165054259229 2 55 -8.076930525604439 2 56 -7.307179237780934 3 1 1.5870924763942824 3 2 3.2445358185927944 3 3 3.872890928863015 3 4 2.5374873322966778 3 5 2.602357614441715 3 6 6.4534470566643165 3 7 2.7066237794312897 3 8 2.7244058244176372 3 9 3.063437574083075 3 10 2.4051129569518963 3 11 2.095639510114765 3 12 2.2468624111552913 3 13 -5.007552789255591 3 14 -5.388069902514296 3 15 -3.1325655288286782 3 16 3.011708239780326 3 17 2.5011016823798116 3 18 1.4212714146124235 3 19 0.7461458461247261 3 20 0.6765221096873617 3 21 2.9172844761343484 3 22 3.1716666946321865 3 23 2.892426768477506 3 24 2.8287044518998528 3 25 3.1299148664858265 3 26 1.802669607157945 3 27 2.2083523695953033 3 28 1.247511503388644 3 29 3.016133690471257 3 30 1.9810974372452108 3 31 1.8079325681493756 3 32 1.1893127309471936 3 33 2.240444802909707 3 34 2.955511345007409 3 35 2.0167481993011123 3 36 4.518030932930156 3 37 4.935962645592138 3 38 5.339957120857928 3 39 7.1085813735973105 3 40 9.724669071410752 3 41 9.697721442189547 3 42 6.9697840978805665 3 43 2.8190256041706263 3 44 3.3178502078985836 3 45 6.183765993124556 3 46 0.28982061575776896 3 47 -4.960767523459175 3 48 -1.131689432476712 3 49 -10.069310711583299 3 50 -0.5391015030928301 3 51 -2.471372692380779 3 52 -2.657625110876637 3 53 -4.158086209641688 3 54 -6.949407028535084 3 55 -3.8405507284696476 3 56 -0.9132129092229491 4 1 2.467770198095799 4 2 3.808823249498829 4 3 4.8504604233755515 4 4 3.047020297128853 4 5 3.626270135470314 4 6 6.834565377580766 4 7 3.358229963842092 4 8 3.505147633277687 4 9 3.642224724607793 4 10 3.475608831341063 4 11 4.268795001583165 4 12 5.949618158972321 4 13 5.233615199208717 4 14 5.759771161332534 4 15 6.521884523242139 4 16 6.60375342122709 4 17 5.858427645689915 4 18 5.230612117968985 4 19 4.7307649280761765 4 20 4.502445365315012 4 21 3.1878524485768764 4 22 3.398094636633303 4 23 3.114328554907054 4 24 4.3478905449864556 4 25 3.6809843209438853 4 26 2.8414823925460766 4 27 3.0355573683936683 4 28 2.384898349499352 4 29 2.868773280313441 4 30 2.704361075793635 4 31 2.979926479869313 4 32 4.094463736314495 4 33 2.950210083691566 4 34 3.117491638206161 4 35 2.644802352950073 4 36 4.595095741069226 4 37 4.790164359923626 4 38 5.240742188549991 4 39 7.49610841036732 4 40 9.700796604856244 4 41 5.868553774161029 4 42 8.384307850183703 4 43 7.964223072727865 4 44 10.23449684732821 4 45 8.562080500855071 4 46 8.974231414443977 4 47 8.480192181739895 4 48 8.471330674869279 4 49 4.255651236666321 4 50 8.119313941912377 4 51 6.9327769511344 4 52 6.565197928456854 4 53 6.073789140834961 4 54 0.004650489689701942 4 55 6.6776340027648295 4 56 5.831519927434904 5 1 2.4975947594993073 5 2 3.870928512656718 5 3 4.406132937995437 5 4 2.9093594784154035 5 5 3.1659192582773343 5 6 6.686963038399476 5 7 3.0769434141344902 5 8 3.32467979702038 5 9 3.2290083503387805 5 10 2.8606380711071466 5 11 4.506689738425273 5 12 6.940378392890452 5 13 6.648702918506967 5 14 7.276496307531364 5 15 7.003872827335709 5 16 6.767963947542881 5 17 6.678544604754244 5 18 5.943828492237994 5 19 4.900940366672671 5 20 5.09917051151384 5 21 3.3479694076412017 5 22 3.573767151253186 5 23 3.1569016543222546 5 24 3.8132706619455163 5 25 3.319229934978978 5 26 2.7081490592127437 5 27 2.7131012280427953 5 28 2.627237530785905 5 29 3.321521818325138 5 30 3.034887391583112 5 31 3.464370924313755 5 32 3.893878940992856 5 33 2.801905990124318 5 34 3.496789883820192 5 35 2.5526386102600114 5 36 3.4984875539347193 5 37 3.5520357049528677 5 38 4.667993650538289 5 39 6.072599638437495 5 40 6.04138140017788 5 41 0.9128812595411482 5 42 9.011793230300661 5 43 11.478842955768801 5 44 9.701046554930546 5 45 8.21143722600127 5 46 9.93785714543813 5 47 9.642063526769139 5 48 9.832968101769861 5 49 9.520095681110767 5 50 10.01112680740945 5 51 9.659677535929724 5 52 9.679817811497788 5 53 10.02127452095192 5 54 9.403013062789114 5 55 9.570499499840857 5 56 8.61409302685011 6 1 2.4741713360758837 6 2 3.9639915757197812 6 3 4.747844649707147 6 4 3.1651252441811693 6 5 3.512946285304359 6 6 7.00083691227335 6 7 3.3841506213416963 6 8 3.592787905128482 6 9 3.643963305293738 6 10 3.4467641972332714 6 11 3.753266315001845 6 12 6.9226306451427035 6 13 6.203387603191654 6 14 8.20208189311695 6 15 7.159548503011383 6 16 6.695621605200536 6 17 6.466022082231724 6 18 6.037432095841599 6 19 4.9428322585645645 6 20 5.370431772775106 6 21 3.4936450833168777 6 22 3.5139473314333713 6 23 2.783207960628566 6 24 4.256694085368942 6 25 3.2765272322762726 6 26 2.4811220321857164 6 27 2.5485967235382887 6 28 2.96021050375888 6 29 3.1438641606674835 6 30 3.457680184375903 6 31 2.782479032421861 6 32 4.407842904956823 6 33 2.9911852694035943 6 34 3.233186280216586 6 35 2.970746718368119 6 36 3.0283974638446303 6 37 2.9576212905384516 6 38 3.1953810379256815 6 39 5.624131169969026 6 40 3.1689489677454468 6 41 -2.314866488206601 6 42 7.126207644715073 6 43 10.271635748561593 6 44 8.471497005380996 6 45 6.153869658433699 6 46 10.145244532825522 6 47 9.18593740064301 6 48 9.71864377744554 6 49 10.661807392822478 6 50 9.128243924526569 6 51 8.46328113953333 6 52 8.422160153840135 6 53 8.919292538969941 6 54 8.259589639365693 6 55 7.641400400741755 6 56 7.272921855678938 7 1 2.486172848077395 7 2 3.1311149928432016 7 3 3.867718019580515 7 4 2.9407347697906943 7 5 3.059345831703908 7 6 6.075680987117423 7 7 2.787666024857103 7 8 2.90076371310429 7 9 2.5887752501056838 7 10 3.0956963461654183 7 11 4.1306524423879765 7 12 6.281683754195811 7 13 6.035602685406733 7 14 7.755190946226005 7 15 6.662194505657386 7 16 6.481437141016073 7 17 5.730386096595737 7 18 4.8038751122846115 7 19 4.744996310728617 7 20 4.60276025510359 7 21 3.155381995053787 7 22 3.2172170347030757 7 23 2.8731720505926504 7 24 3.716720545395404 7 25 2.8533558091048477 7 26 2.403253954317634 7 27 2.7360849110264738 7 28 1.5466780902264645 7 29 2.2269297437330664 7 30 2.5978710745667897 7 31 2.9348604348032694 7 32 3.8046015517154714 7 33 1.7497847780031037 7 34 2.6083516553819663 7 35 2.4203309179523202 7 36 1.627811563258728 7 37 1.1820590149761792 7 38 1.4560122985569386 7 39 2.9550705009083593 7 40 -1.5664507676542883 7 41 -10.069449742789855 7 42 5.010161528668959 7 43 8.504483981409827 7 44 6.320580354464347 7 45 4.153255407819451 7 46 7.398882786463773 7 47 7.440851405557016 7 48 7.688072996874759 7 49 9.36210034311543 7 50 7.424879721162366 7 51 8.895808072060255 7 52 8.816414548094524 7 53 9.3826265023039 7 54 9.701661081437138 7 55 10.09656010590146 7 56 9.140479973237056 8 1 2.479129531681565 8 2 4.099369759659119 8 3 5.011552602264018 8 4 3.4862899340508946 8 5 4.054264582018341 8 6 6.906339537200438 8 7 3.4047611599378484 8 8 3.675327279034768 8 9 3.8856510361901044 8 10 3.595218406838562 8 11 5.450670553772994 8 12 7.325654172266951 8 13 7.839446323782749 8 14 8.963019089305948 8 15 7.566606640285347 8 16 7.671656993106431 8 17 7.106650120341776 8 18 6.8265143435569335 8 19 5.8153288558813045 8 20 5.360369552281227 8 21 4.014012573108829 8 22 4.0579877747543875 8 23 4.313832109957751 8 24 4.881232292640959 8 25 4.051172381022141 8 26 2.9541922246803693 8 27 3.52312520885814 8 28 2.965654796972956 8 29 3.6804187008191462 8 30 3.6510984227581673 8 31 4.021493226472032 8 32 5.018818988954486 8 33 3.7628652227382275 8 34 3.719283888616353 8 35 3.5370990419146864 8 36 1.4290391126877218 8 37 1.732946975936077 8 38 2.4866507248788174 8 39 4.342000118053804 8 40 -1.2978991753616889 8 41 -8.935224256046384 8 42 5.829299225504496 8 43 9.797787799893499 8 44 8.115770775554047 8 45 5.374314923842995 8 46 9.580399111864992 8 47 9.096835709023335 8 48 9.476421339179932 8 49 10.803213187105971 8 50 8.73654647167804 8 51 10.002363387248664 8 52 9.113390952984599 8 53 10.096718166035856 8 54 10.498360784611663 8 55 9.852609811591456 8 56 8.285220125171453 9 1 2.511069936804276 9 2 3.7076661013092007 9 3 4.559182583385507 9 4 2.9188630245146925 9 5 3.0887561377099573 9 6 6.502849563222171 9 7 2.9783618538507994 9 8 2.8641124211338536 9 9 3.249292038282043 9 10 2.91992885124899 9 11 4.483427327077749 9 12 7.005484775869178 9 13 6.807710010705545 9 14 7.925006945829235 9 15 7.395220345066203 9 16 7.259595153216637 9 17 6.628615526740056 9 18 6.105814307840827 9 19 4.886046749651392 9 20 5.192503844847174 9 21 3.0998843012582213 9 22 3.8869586406148926 9 23 3.4627172571591345 9 24 4.095682009463246 9 25 3.4544072399435137 9 26 2.593255442191463 9 27 2.8190586748513056 9 28 2.4127694456795226 9 29 3.0958480594598896 9 30 3.014178171724957 9 31 3.2967113498456726 9 32 3.8463612104964042 9 33 3.1037499617555255 9 34 3.3119671887847346 9 35 2.5381705251536317 9 36 2.0227428730836543 9 37 1.9996952794209513 9 38 2.3824617356446716 9 39 4.737989709359482 9 40 -0.6752143445029724 9 41 -5.130948527692897 9 42 6.3713676983857646 9 43 9.797991891939013 9 44 8.159344427270973 9 45 5.638103892667935 9 46 9.481261400757283 9 47 9.313836576414527 9 48 9.665308527301779 9 49 11.636265893876722 9 50 9.035949502444911 9 51 8.37386193309284 9 52 9.172016393058076 9 53 9.19560076208667 9 54 9.47733930392387 9 55 10.150073967925962 9 56 9.343029197062876 10 1 2.467770198095799 10 2 3.8865174850878006 10 3 4.796158000652078 10 4 3.2232943155081344 10 5 3.658851589104401 10 6 6.881349003311755 10 7 3.09924917854552 10 8 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2.147891089263247 115 6 2.3335358083525293 115 7 1.336567827280028 115 8 1.6184826139217847 115 9 1.613890979446765 115 10 1.5577272729850762 115 11 -1.578005097255481 115 12 -6.211077006170584 115 13 -2.35571022703294 115 14 -9.526696180731545 115 15 -2.5513384402699266 115 16 -11.970252014898435 115 17 -11.122957742663598 115 18 -3.0884719772455753 115 19 -4.311735689665358 115 20 -3.8826135260448424 115 21 -0.05174890221795181 115 22 -0.2108337877139448 115 23 -0.17568050530215373 115 24 0.20416268072486332 115 25 -0.006685557978769907 115 26 0.6349096225930211 115 27 0.7526317444747193 115 28 0.9168149955746401 115 29 1.080864541329838 115 30 1.5158263587192593 115 31 0.08634275529966828 115 32 -4.2239614345939955 115 33 0.19664777416187462 115 34 0.7006396490784113 115 35 0.23939917364029384 115 36 3.5245908403197 115 37 3.5668713857040366 115 38 5.310341068378666 115 39 6.519829685385851 115 40 8.671803935389143 115 41 7.66058079005758 115 42 0.9747040284227246 115 43 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1.6857516741094116 116 26 1.9755403635605688 116 27 1.8429563005065646 116 28 2.008976661220686 116 29 1.9573189197744156 116 30 1.8895250727425292 116 31 0.7287187504007093 116 32 -8.541628305383952 116 33 0.990891497370697 116 34 0.6447608983129456 116 35 0.9713342624339276 116 36 2.8395020466883416 116 37 3.1039197629238835 116 38 3.869587853436844 116 39 4.998396739886772 116 40 7.590511834960488 116 41 6.929258071135351 116 42 2.784256998416602 116 43 -9.557388928289171 116 44 -0.7796780827506105 116 45 0.5583937477403964 116 46 -4.4414182168807095 116 47 -9.148081400767095 116 48 -6.272249289534486 116 49 -4.1948318551211194 116 50 -3.2207572505615616 116 51 -1.078728261171726 116 52 -1.7100372609659757 116 53 -2.4211892471640226 116 54 -7.266262299529723 116 55 -2.48051499291277 116 56 -0.03054465430930975 117 1 0.4048411363108997 117 2 2.0712183677291875 117 3 2.597727140893987 117 4 1.3305188987052574 117 5 1.5511366495816858 117 6 1.8516007195588955 117 7 0.5392622547142025 117 8 1.006418927455158 117 9 0.6527764662808124 117 10 1.3133916942955537 117 11 -10.413889971719659 117 12 -13.268027404210997 117 13 -10.506659400333614 117 14 -12.282054417106316 117 15 -11.90743152049038 117 16 -14.291746197384658 117 17 -10.920875685100828 117 18 -5.1132729570373705 117 19 -4.4381900681099395 117 20 -5.321988908776014 117 21 -0.6929001575761902 117 22 -0.10826183425405844 117 23 0.07110455287298123 117 24 -2.8913670192139023 117 25 -0.9120744128471117 117 26 1.0298881896475223 117 27 0.7994780396369947 117 28 0.5741940525250371 117 29 1.555145006730939 117 30 1.0743076814381851 117 31 -0.29302038003407205 117 32 -13.422063087992651 117 33 -0.11780415480321693 117 34 -0.14871736255661716 117 35 -1.1917092158269398 117 36 1.11124117712313 117 37 1.2343545455325788 117 38 1.597848723002059 117 39 3.476657609451987 117 40 3.4274683566996167 117 41 4.179258071135351 117 42 1.6103439549383403 117 43 -8.535649797854388 117 44 -4.0296780827506105 117 45 1.145350269479529 117 46 -5.908809521228534 117 47 -10.713298792071443 117 48 -8.011379724317095 117 49 -7.37961446381677 117 50 -5.568583337518085 117 51 -3.3939456524760736 117 52 -3.6230807392268467 117 53 -4.105971855859675 117 54 -7.6140883864862445 117 55 -4.415297601608422 117 56 -3.5957620456136574 118 1 1.1551705170750637 118 2 2.9691103308385394 118 3 3.4376480895105885 118 4 1.846329175385101 118 5 2.4253131976712723 118 6 3.9602963717328095 118 7 1.6920949292860072 118 8 2.07558888792947 118 9 2.0071901685206015 118 10 1.7145774650465384 118 11 -6.346037534302003 118 12 -9.51901554650349 118 13 -6.185842536038489 118 14 -0.18653399549893734 118 15 -17.849460505997627 118 16 -7.090823931244998 118 17 -12.50387963767 118 18 -3.907080598671101 118 19 -2.577847512115209 118 20 -3.171132518789186 118 21 1.1740300137018096 118 22 1.015585333071371 118 23 0.8556895331101373 118 24 -2.1212747925999373 118 25 1.130745086494123 118 26 1.4475429986066786 118 27 2.1743133492549163 118 28 1.9266314701798422 118 29 2.195461212264533 118 30 2.369432846128568 118 31 1.2274012273440604 118 32 -7.200060452946538 118 33 1.7194817477000726 118 34 1.9428504898807972 118 35 2.1126386102600136 118 36 3.3912148266619973 118 37 3.4629447958619544 118 38 4.338296680841321 118 39 5.759266305104163 118 40 8.237745036541511 118 41 7.932881259541148 118 42 2.696641715149145 118 43 1.0697520466778911 118 44 3.187713221597214 118 45 4.910831165395205 118 46 -2.2572943697133816 118 47 -7.3318758671702575 118 48 -5.934304625502865 118 49 -2.875661894646811 118 50 -1.5191762228935772 118 51 -0.7463830701308822 118 52 -1.4807882491082687 118 53 -3.356301236623839 118 54 -9.089714209938155 118 55 -5.070106560765208 118 56 -1.502876670119587 119 1 1.1012984632030154 119 2 2.8546322163604287 119 3 2.860207012069509 119 4 1.8715817006376199 119 5 2.189622961981037 119 6 3.3677037791402142 119 7 1.0102767474678203 119 8 1.206902019242598 119 9 1.3001194614498957 119 10 0.7465639970330713 119 11 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-8.74434876333368 119 50 -8.877762081479435 119 51 -9.918100241848055 119 52 -10.086848855168876 119 53 -13.580206960529562 119 54 -13.187357307581253 119 55 -14.157648648307294 119 56 -11.620721787964705 120 1 0.8436041491706732 120 2 2.674402691060479 120 3 3.091297257244264 120 4 1.940439290621974 120 5 2.204229117432263 120 6 3.8546625689159058 120 7 1.2802297991110123 120 8 1.195947402654177 120 9 1.205440275221413 120 10 1.388713188478036 120 11 -11.535751576128721 120 12 -2.5913586963114277 120 13 -5.3416257199906845 120 14 -8.695710265238588 120 15 -6.9457046374530265 120 16 -12.63222384588435 120 17 -12.306056334212894 120 18 -3.4265001462596594 120 19 -3.227228647411838 120 20 -5.136134652805406 120 21 -0.4461150994010552 120 22 -0.32350984405197636 120 23 -1.1052579700908893 120 24 -5.964851403782184 120 25 -0.31654471290834607 120 26 0.9165997634380929 120 27 0.9779838571507753 120 28 0.9872375307859045 120 29 1.3766391892171583 120 30 0.12146016153616301 120 31 -2.786896681320048 120 32 -12.533820589523575 120 33 -2.2681409582324896 120 34 -8.961332181907508 120 35 -3.0000374460780144 120 36 -1.3768176103845278 120 37 -0.6162272058452558 120 38 -0.9009265372551347 120 39 0.9987029248224708 120 40 -1.5253791632024019 120 41 0.9704399449871559 120 42 -6.785859351858964 120 43 -3.6526124432922344 120 44 -4.657357200937998 120 45 -9.90180221061845 120 46 -14.89472501418628 120 47 -14.30178623848908 120 48 -11.869003729216054 120 49 -15.896148450344633 120 50 -10.887934225454398 120 51 -10.997129975807367 120 52 -10.64788171901864 120 53 -10.706706699705357 120 54 -12.434357829229661 120 55 -11.091660124572291 120 56 -7.660367067046604 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1287.0 5 44.0 6 142.0 7 149.0 8 155.0 9 129.0 10 82.0 11 128.0 12 144.0 13 154.0 14 229.0 15 366.0 16 535.0 17 659.0 18 874.0 19 1043.0 20 1248.0 21 1654.0 22 1972.0 23 2317.0 24 2529.0 25 2782.0 26 2833.0 27 2908.0 28 3217.0 29 3354.0 30 3841.0 31 4353.0 32 5300.0 33 6684.0 34 7464.0 35 9087.0 36 10549.0 37 11276.0 38 7037.0 39 1556.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 100.0 0.0 0.0 7 99.37398680682293 0.32728051304534006 0.2763022399853183 0.0224304401464096 8 99.5095890131626 0.41904140455337935 0.043841314831618766 0.027528267452411784 9 98.1642955484167 0.9698638519198409 0.2559787874152261 0.609861812248228 10 32.31614685820903 44.907780304034425 13.887501147011145 8.888571690745405 11 21.527544122595053 29.81168241927426 35.10231548037806 13.558457977752628 12 46.154708863082554 10.745200395591398 14.867303555224765 28.232787186101284 13 19.10563717743498 25.663482223876183 17.747575983115997 37.48330461557284 14 36.32126941393622 13.01434821182733 25.748258736908657 24.916123637327786 15 25.581039130789968 29.43182746507638 18.887345805081285 26.099787599052366 16 28.260625656613048 13.34645709448088 38.71237543476709 19.680541814138984 17 40.561679736495925 15.787809877323761 24.901338935174326 18.749171451005985 18 39.810151104223166 19.344807194273944 24.558004853280043 16.287036848222844 19 28.54083599318805 38.478325158316586 14.530454911638435 18.450383936856916 20 19.296635149883215 26.33130361168059 16.429525820303336 37.94253541813286 21 18.737445320227184 17.796290442638497 45.8106881748937 17.655576062240623 22 18.12110645500056 17.99161900100939 29.249890394477923 34.63738414951213 23 19.939641724696937 38.011439524474675 18.462291371417503 23.586627379410896 24 18.01470213395051 36.95822840305462 26.47811502737533 18.54895443561954 25 27.386547853304922 38.28774176445999 17.511036796117494 16.814673586117596 26 27.68635142451452 18.808290684727204 29.576169899788546 23.929187990969734 27 36.97856108357302 16.787018338330988 19.956958978438692 26.277461599657304 28 17.398588254522796 44.9317111540151 21.438713697608613 16.230986893853494 29 25.325324304479075 37.068613853308314 22.00579260830546 15.600269233907154 30 16.910084856396868 18.83159268929504 50.30495267624021 13.953369778067884 31 16.55817945861988 25.963356188880148 43.68338766209215 13.795076690407823 32 43.64331599877638 16.390333435301315 24.154175588865094 15.812174977057204 33 17.16299739490218 36.045359350257954 25.178525821116615 21.613117433723247 34 15.943833295916077 25.91587240035104 27.086352225646472 31.053942078086415 35 34.97822339633419 19.09609245111739 34.79564671922971 11.130037433318714 36 33.93719683431197 27.590502935920348 25.310186367117694 13.162113862649989 37 32.27201177504753 19.458470470388615 35.09209477277838 13.177422981785472 38 24.773293574608882 21.338997589967732 42.46456435603121 11.423144479392182 39 15.00465792410143 31.84996365795481 23.391992465423872 29.753385952519885 40 10.176432552267759 14.489228274368527 24.03444488920364 51.299894284160075 41 23.776533322352844 15.143810092441992 26.565234399127053 34.514422186078114 42 20.489843462623124 22.67646217353987 28.0071116197327 28.8265827441043 43 9.150259915680897 20.35016986615366 26.377348450738815 44.12222176742663 44 9.661682262147565 14.262434534851545 21.023664818439904 55.052218384560994 45 14.097892104322973 36.854999234420454 35.30546623794212 13.741642423314449 46 30.10712099571516 20.32442358702306 28.87982044480718 20.6886349724546 47 13.082853995838942 23.60584179822951 43.33210949292212 19.979194713009424 48 15.368764663878403 26.779557278384168 44.395593185759466 13.456084871977966 49 9.643470790378007 17.592251677303224 20.636966126656848 52.12731140566193 50 20.6354234793983 20.123446697187706 46.42127207325049 12.819857750163505 51 31.724413155148067 27.349937580582445 28.735443996479955 12.190205267789533 52 15.27540356013225 37.920833631887646 33.22960703428086 13.574155773699243 53 31.102551265415386 21.644375874858234 34.27400815342331 12.979064706303067 54 12.89176552259224 24.84839822773955 39.74927006554097 22.510566184127242 55 15.029616013071895 26.958741830065357 28.485498366013072 29.526143790849673 56 38.694422029968436 20.659223925722397 25.85824744900565 14.788106595303516 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 2.5 5 4.0 6 2.5 7 1.0 8 23.0 9 45.0 10 84.0 11 123.0 12 142.5 13 220.5 14 279.0 15 349.0 16 419.0 17 510.5 18 602.0 19 679.0 20 756.0 21 992.0 22 1508.5 23 1789.0 24 2067.0 25 2345.0 26 2524.0 27 2703.0 28 2850.5 29 2976.0 30 2954.0 31 2745.5 32 2537.0 33 2313.5 34 2090.0 35 1944.0 36 1798.0 37 1730.5 38 1717.0 39 1771.0 40 1908.0 41 2045.0 42 3116.5 43 4188.0 44 6285.0 45 8382.0 46 8605.0 47 8114.5 48 7401.0 49 6553.5 50 5706.0 51 5426.5 52 5147.0 53 4930.5 54 4432.0 55 4150.0 56 4033.5 57 3917.0 58 3831.5 59 3746.0 60 3532.5 61 3319.0 62 3142.5 63 2593.5 64 2221.0 65 1978.0 66 1735.0 67 1497.0 68 1259.0 69 1038.5 70 818.0 71 715.5 72 477.0 73 341.0 74 257.0 75 173.0 76 129.0 77 85.0 78 64.0 79 27.5 80 12.0 81 7.0 82 2.0 83 1.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.026508701991211348 10 0.0 11 0.002039130922400873 12 0.0 13 0.0 14 0.020391309224008728 15 0.15599351556366678 16 0.040782618448017456 17 0.018352178301607854 18 0.003058696383601309 19 0.018352178301607854 20 0.03874348752561658 21 0.010195654612004364 22 0.002039130922400873 23 0.0 24 0.0 25 0.0 26 0.19167830670568203 27 0.03568479114201527 28 0.19167830670568203 29 0.02548913653001091 30 0.033645660219614404 31 0.224304401464096 32 0.0112152200732048 33 0.19983483039528552 34 0.08870219512443797 35 0.040782618448017456 36 0.15905221194726807 37 0.2518326689165078 38 0.1600717774084685 39 0.40578705355777367 40 0.6627175497802836 41 0.9573719680672098 42 0.21716744323569295 43 0.3639848696485558 44 0.5199783852122225 45 0.11826959349925062 46 0.06219349313322662 47 0.029567398374812655 48 0.05199783852122225 49 0.3109674656661331 50 0.22940222877009817 51 0.36194573872615493 52 0.3955913989457693 53 0.21206961592969079 54 0.12948481357245542 55 0.16415003925327024 56 0.18148265209367767 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 98081.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.94500463902286 #Duplication Level Percentage of deduplicated Percentage of total 1 92.79017180045777 68.61369684240577 2 4.386013291785015 6.486475464157176 3 1.206463888812288 2.6763593356511453 4 0.5129195047293384 1.5171134062662492 5 0.28679370156909245 1.060348079648454 6 0.15994264125968619 0.7096175609955038 7 0.108926453961338 0.5638197000438413 8 0.08962303174034139 0.530174039824227 9 0.049637371425419845 0.3303392094289414 >10 0.33229462537572735 4.633925021155983 >50 0.038606844441993214 2.0411700533232735 >100 0.024818685712709922 4.707333734362415 >500 0.013788158729283292 6.129627552737023 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 799 0.8146328034991487 No Hit TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 650 0.6627175497802836 No Hit TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 640 0.6525218951682793 No Hit TATGTAGGGATGAGATGGACTTAAATGAATTGACGGGTACCCACAGTTCTGAGTCG 604 0.6158175385650636 No Hit TATGTAGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG 581 0.5923675329574535 No Hit TATGTAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG 579 0.5903284020350527 No Hit TATGTAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG 563 0.5740153546558457 No Hit TATGTAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG 544 0.5546436108930374 No Hit TATGTAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG 527 0.53731099805263 No Hit TATGTAGGGATGAGATGGACTCAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG 525 0.5352718671302291 No Hit TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 487 0.4965283796046125 No Hit TATGTAGGGATGAGATGGACTCAAATGAATTGACGGGTACCCACAGTTCTGAGTCG 447 0.45574576115659504 No Hit TATGTAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG 406 0.4139435772473772 No Hit TATGTAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG 399 0.4068066190189741 No Hit TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG 370 0.37723922064416143 No Hit TATGTAGGGATGAGATGGACTCAAATGAATTGACGGGTACGCACAGTTCTGAGTCG 353 0.35990660780375405 No Hit TATGTAGGGATGAGATGGACTCAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG 332 0.3384957331185449 No Hit TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAACGATTAAG 270 0.2752826745241178 No Hit TATGTAGGGATGCGCTGGACTTAAAGGAATTGACGGGTCCGCCCAGTTCTGAGTCG 223 0.22736309784769732 No Hit TATGTAGGGATGCGCTGGACTTAAATGAATTGACGGGTCCGCCCAGTTCTGAGTCG 214 0.2181870086968934 No Hit TATGTAGGGATGCGCTGGACTCAAAGGAATTGACGGGTCCGCCCAGTTCTGAGTCG 181 0.184541348477279 No Hit TATGTAGGGATGCGCTGGACTCAAATGAATTGACGGGTCCGCCCAGTTCTGAGTCG 156 0.15905221194726807 No Hit TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTCAG 149 0.15191525371886502 No Hit TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGCCTCAAAGATTAAG 148 0.15089568825766458 No Hit TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC 136 0.13866090272325934 No Hit TATGTAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC 136 0.13866090272325934 No Hit TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTCAG 107 0.1090935043484467 No Hit TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAAAGATTAAG 103 0.10501524250364495 No Hit TATGTAGGGATGCGCTGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG 99 0.10093698065884321 No Hit TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG 99 0.10093698065884321 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0010195654612004364 0.0 0.0 0.0 0.0 7 0.0010195654612004364 0.0 0.0 0.0 0.0 8 0.002039130922400873 0.0 0.0 0.0 0.0 9 0.003058696383601309 0.0 0.0 0.0 0.0 10 0.004078261844801746 0.0 0.0 0.0 0.0 11 0.012234785534405237 0.0 0.0 0.0 0.0 12 0.013254350995605672 0.0 0.0 0.0 0.0 13 0.021410874685209164 0.0 0.0 0.0 0.0 14 0.024469571068810474 0.0 0.0 0.0 0.0 15 0.027528267452411784 0.0 0.0 0.0 0.0 16 0.02854783291361222 0.0 0.0 0.0 0.0 17 0.031606529297213526 0.0 0.0 0.0 0.0 18 0.03670435660321571 0.0 0.0 0.0 0.0 19 0.0448608802928192 0.0 0.0 0.0 0.0 20 0.05301740398242269 0.0 0.0 0.0 0.0 21 0.05505653490482357 0.0 0.0 0.0 0.0 22 0.05913479674962531 0.0 0.0 0.0 0.0 23 0.06627175497802837 0.0 0.0 0.0 0.0 24 0.06831088590042923 0.0 0.0 0.0 0.0 25 0.07238914774523099 0.0 0.0 0.0 0.0 26 0.07544784412883229 0.0 0.0 0.0 0.0 27 0.07952610597363403 0.0 0.0 0.0 0.0 28 0.08870219512443797 0.0 0.0 0.0 0.0 29 0.09176089150803927 0.0 0.0 0.0 0.0 30 0.09481958789164058 0.0 0.0 0.0 0.0 31 0.09481958789164058 0.0 0.0 0.0 0.0 32 0.09481958789164058 0.0 0.0 0.0 0.0 33 0.09481958789164058 0.0 0.0 0.0 0.0 34 0.09481958789164058 0.0 0.0 0.0 0.0 35 0.09481958789164058 0.0 0.0 0.0 0.0 36 0.09481958789164058 0.0 0.0 0.0 0.0 37 0.09481958789164058 0.0 0.0 0.0 0.0 38 0.09481958789164058 0.0 0.0 0.0 0.0 39 0.09583915335284102 0.0 0.0 0.0 0.0 40 0.09583915335284102 0.0 0.0 0.0 0.0 41 0.09583915335284102 0.0 0.0 0.0 0.0 42 0.09991741519764276 0.0 0.0 0.0 0.0 43 0.10297611158124408 0.0 0.0 0.0 0.0 44 0.10705437342604582 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACAACC 15 0.008048538 50.55544 38 CAGCCCA 15 0.008048538 50.55544 39 TAAACAC 20 3.9437826E-4 50.55544 38 CCGTCAT 15 0.008048538 50.55544 39 TAACCAC 40 2.39379E-9 50.55544 38 TACGACC 45 1.200533E-10 50.55544 38 ACACTAG 15 0.008048538 50.55544 40 CGTCATC 15 0.008048538 50.55544 40 TAGTCTC 20 3.9437826E-4 50.55544 40 CTTCAAT 20 3.9741548E-4 50.47698 41 CACTAGT 15 0.00809814 50.47698 41 AAACACC 15 0.00809814 50.47698 41 CAGTAGT 20 3.9741548E-4 50.47698 41 GTACGAC 50 5.456968E-12 50.42481 37 TGCTCGT 60 0.0 50.424805 37 GTACGCG 20 3.994506E-4 50.424805 37 GTACACA 35 4.8854417E-8 50.4248 37 GATACGC 25 2.0023726E-5 50.294846 36 CAAGAGA 15 0.008214762 50.29484 36 TATGCTA 20 4.0560565E-4 50.268936 35 >>END_MODULE