FastQCFastQC Report
Fri 17 Jun 2016
SRR1526312_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526312_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48208
Sequences flagged as poor quality0
Sequence length56
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC1310.2717391304347826No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1180.2447726518420179No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC910.1887653501493528No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTCCTCTTCC730.1514271490209094No Hit
GGTACAGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG680.14105542648523067No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGACCACCCGACTGCTCTTC640.1327580484566877No Hit
GGTACAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG590.12238632592100895No Hit
GGTACAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG550.11408894789246599No Hit
GGTACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTT510.105791569863923No Hit
GGTACAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG510.105791569863923No Hit
GGTACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGCCCACCCGACTGCTCTT500.10371722535678725No Hit
GGTACAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG490.10164288084965152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCCA402.3192115E-950.62473739
TACGCCC402.3192115E-950.62473738
CTGTCTC150.00797999450.62473740
CGGACAA150.00801344250.5712837
TGTCTCA203.9172094E-450.57127841
GTACGCC451.1641532E-1050.57127837
TATGCCT150.00814828150.3585735
ATGCCTG150.00814828150.3585736
GTAAGCA150.00814828150.3585735
GGTAAGC204.0205452E-450.3056734
CCTGAAC150.00825051150.2002143
CCAAGAC150.00825051150.2002143
ACAGTTC850.050.2002143
CTGAACG150.008284850.14764844
CATATGC451.2732926E-1050.14764433
CAACACA204.0835785E-450.1476444
TCAAAGA355.0293238E-850.1476445
AAAGATT355.0293238E-850.1476447
GTGGCAA150.00842301349.9384830
CGATGGA150.00842301349.9384813