##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526312_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 48208 Sequences flagged as poor quality 0 Sequence length 56 %GC 55 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.62987470959177 38.0 36.0 39.0 32.0 40.0 2 36.19928227680053 38.0 36.0 39.0 30.0 40.0 3 35.35564636574842 38.0 35.0 39.0 28.0 40.0 4 34.133318121473614 38.0 35.0 39.0 17.0 40.0 5 35.73525141055426 38.0 35.0 39.0 28.0 40.0 6 33.327808662462665 38.0 33.0 39.0 13.0 40.0 7 35.413624294722865 38.0 35.0 39.0 27.0 40.0 8 35.51433372054431 38.0 35.0 39.0 28.0 40.0 9 35.54403833388649 38.0 35.0 39.0 27.0 40.0 10 35.26798456687687 38.0 35.0 39.0 27.0 40.0 11 34.572705774975105 38.0 33.0 39.0 26.0 40.0 12 32.207621141719216 36.0 29.0 39.0 16.0 40.0 13 31.721332558911385 36.0 28.0 39.0 15.0 40.0 14 31.35846747427813 36.0 27.0 39.0 14.0 40.0 15 31.94330816461998 36.0 28.0 39.0 16.0 40.0 16 32.52453949551941 36.0 30.0 39.0 18.0 40.0 17 32.55266760703618 36.0 30.0 39.0 18.0 40.0 18 32.79883006969798 36.0 30.0 38.0 20.0 40.0 19 33.51815051443744 36.0 31.0 38.0 24.0 40.0 20 33.80339362761367 37.0 31.0 39.0 24.0 40.0 21 35.209031695984066 38.0 35.0 39.0 27.0 40.0 22 34.883276634583474 38.0 35.0 39.0 26.0 40.0 23 35.0767714902091 38.0 35.0 39.0 27.0 40.0 24 34.69231247925656 38.0 34.0 39.0 25.0 40.0 25 34.97019166943246 38.0 34.0 39.0 27.0 40.0 26 35.24431629605045 38.0 35.0 39.0 28.0 40.0 27 35.152733986060404 38.0 35.0 39.0 27.0 40.0 28 35.0039827414537 38.0 35.0 39.0 27.0 40.0 29 34.9214238300697 38.0 35.0 39.0 27.0 40.0 30 34.681090275472954 38.0 35.0 39.0 26.0 40.0 31 34.40186276136741 38.0 34.0 39.0 26.0 40.0 32 33.21546216395619 38.0 33.0 39.0 18.0 40.0 33 34.53466229671424 38.0 34.0 39.0 26.0 40.0 34 34.223178725522736 38.0 33.0 39.0 25.0 40.0 35 34.2704945237305 38.0 33.0 39.0 26.0 40.0 36 32.441565715233985 36.0 31.0 38.0 22.0 39.0 37 32.28978592764686 35.0 31.0 38.0 21.0 39.0 38 31.486869399269832 35.0 30.0 38.0 16.0 39.0 39 29.564844009293065 34.0 27.0 38.0 7.0 39.0 40 25.820237305011617 31.0 13.0 37.0 4.0 39.0 41 22.624605874543644 26.0 10.0 33.0 4.0 37.0 42 25.839902090939262 30.0 21.0 34.0 4.0 37.0 43 22.180405741785595 26.0 9.0 33.0 4.0 37.0 44 23.133214404248257 28.0 10.0 33.0 4.0 37.0 45 25.577269332890808 30.0 21.0 34.0 4.0 37.0 46 25.426609691337536 30.0 17.0 36.0 4.0 38.0 47 24.79934865582476 30.0 11.0 35.0 4.0 38.0 48 24.763255061400596 30.0 13.0 35.0 4.0 38.0 49 22.27628194490541 27.0 6.0 35.0 4.0 38.0 50 24.669432459342847 30.0 11.0 35.0 4.0 38.0 51 26.109234981745768 32.0 7.0 38.0 4.0 39.0 52 25.898730501161634 32.0 4.0 38.0 4.0 39.0 53 25.585048124792564 32.0 4.0 37.0 4.0 39.0 54 24.744482243611017 31.0 4.0 37.0 4.0 39.0 55 24.913873216063724 31.0 4.0 37.0 4.0 39.0 56 24.277173913043477 31.0 4.0 37.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.3997755215405903 1 2 1.9403371428220453 1 3 1.5982353555385131 1 4 4.862933057097067 1 5 2.261183684455702 1 6 5.721560628541464 1 7 3.16622317747305 1 8 3.0533906338854067 1 9 3.089959386109946 1 10 2.5758814862395525 1 11 -3.4882165965967857 1 12 -7.472311562620579 1 13 -20.522759397127732 1 14 -18.165647109175318 1 15 -12.419913200855675 1 16 -22.048169953844738 1 17 -21.95244644479639 1 18 -20.70479554135609 1 19 -7.157454765934286 1 20 -11.777061550015887 1 21 -9.950966560133594 1 22 -4.35980973567511 1 23 -4.568054573022323 1 24 -4.336033936710503 1 25 -4.201056927366391 1 26 1.9657885829129569 1 27 1.4644690888741607 1 28 1.7447157892279321 1 29 1.930562696141422 1 30 2.084186757816255 1 31 0.6780054189815488 1 32 -12.278161392365998 1 33 -0.4037677443590937 1 34 0.7869404938941216 1 35 0.08192154920077854 1 36 1.261443057088517 1 37 1.645329069624168 1 38 2.515671864535527 1 39 4.37466706582541 1 40 6.914960476741012 1 41 -7.261962497577779 1 42 -12.894987652138353 1 43 -14.386939083157674 1 44 -16.586169125121724 1 45 -15.993357492765 1 46 -19.16191275027818 1 47 -18.272751418073483 1 48 -17.98524320333223 1 49 -15.674218516668091 1 50 -17.741390652833587 1 51 -19.185368180585833 1 52 -18.88048112565723 1 53 -18.49595281588917 1 54 -18.772789947158252 1 55 -18.246375379278447 1 56 -17.84007574199449 2 1 1.878661415520824 2 2 2.3874770050562475 2 3 1.6287834238224335 2 4 5.2663472529640885 2 5 2.5894256730750485 2 6 6.335516902875096 2 7 3.4645160795395356 2 8 3.3675565518249044 2 9 3.522124705067718 2 10 3.1781576168175647 2 11 -1.935655949696514 2 12 -9.839487363758643 2 13 -11.503591981733901 2 14 -14.739771098393646 2 15 -7.387268696513058 2 16 -2.6693140089510585 2 17 -1.4954233839997428 2 18 -9.357386137342914 2 19 -2.5644628521876562 2 20 -3.3137491810431428 2 21 2.6270508103366055 2 22 2.7686718456366606 2 23 2.5712086794484748 2 24 2.8223967311541287 2 25 2.6650706557423263 2 26 2.874144378061203 2 27 2.5737832547920974 2 28 2.613838280991928 2 29 3.2252617078215664 2 30 3.3106018521558767 2 31 -0.7337945211202737 2 32 -9.022096702339045 2 33 -0.7859180887735917 2 34 0.3128464537623472 2 35 -0.49489785780730955 2 36 4.13266198491122 2 37 4.547096065730784 2 38 4.786711097838911 2 39 7.891288808862246 2 40 9.285131186534365 2 41 7.240283683913685 2 42 1.6225325634950707 2 43 -1.8966725362873547 2 44 -9.225582123025287 2 45 1.2609102520687827 2 46 -11.490454229763056 2 47 -11.553973340804841 2 48 -9.216150660654781 2 49 -10.623904051259286 2 50 -8.672807244627538 2 51 -7.21112439502129 2 52 -7.229687474863578 2 53 -7.0227572483539795 2 54 -14.540684526373584 2 55 -7.301481698535708 2 56 -6.542980803390115 3 1 2.1547313371603707 3 2 2.480103085024105 3 3 2.2767641350942824 3 4 5.4664678075150235 3 5 2.7620196051626493 3 6 6.602143384691448 3 7 3.642936814922294 3 8 3.7706106004485775 3 9 3.573360389214791 3 10 2.8854109822987795 3 11 1.86000408646985 3 12 2.3132150674247995 3 13 -5.417194553564322 3 14 -5.933261152643194 3 15 -2.109189532357945 3 16 2.332293385061398 3 17 3.017938078728811 3 18 2.661098893800343 3 19 1.4264955564922701 3 20 1.0899076403351984 3 21 3.3994036333223363 3 22 3.6559533405130935 3 23 3.2903165757715627 3 24 4.083598258178441 3 25 3.4504835550792734 3 26 2.993493383486161 3 27 2.982262258207811 3 28 2.6809469810120206 3 29 3.2656474823845514 3 30 3.2599689407634713 3 31 1.9127797203906738 3 32 1.6325145092341913 3 33 2.414001541525785 3 34 2.381160699291783 3 35 1.8504909306194577 3 36 4.121008378320905 3 37 4.1834231704123255 3 38 5.457396249536721 3 39 7.369890376071414 3 40 10.072352404135323 3 41 7.926239078729839 3 42 6.816022617744618 3 43 2.85237307351775 3 44 3.012111266566837 3 45 6.764225502219475 3 46 -0.7241291343240377 3 47 -5.263436873053184 3 48 -1.0817323363630393 3 49 -10.210401941554645 3 50 -2.4320999912620564 3 51 -4.502062711276061 3 52 -4.310860872492672 3 53 -4.527478968265569 3 54 -9.86115870760726 3 55 -5.755570507054898 3 56 -2.5076181351160542 4 1 2.388361591887673 4 2 2.5338614847740644 4 3 2.020317815356819 4 4 5.850121503405006 4 5 3.079725496708207 4 6 6.626522193880387 4 7 3.635591917282042 4 8 3.5474507317190884 4 9 3.8484033646797116 4 10 2.275159380485995 4 11 3.1595821455415773 4 12 4.982382124777509 4 13 6.477007665532412 4 14 4.043297684675135 4 15 6.745006435762075 4 16 5.727511387874337 4 17 6.109514887605194 4 18 5.693760247136801 4 19 4.0722214511633155 4 20 4.402300201672908 4 21 3.665851515803979 4 22 3.310118053749534 4 23 3.1796742879140893 4 24 3.7447953202193816 4 25 3.1394974635024653 4 26 2.9094177466679056 4 27 3.1469754946510093 4 28 2.23994057376089 4 29 2.592104741331262 4 30 2.6562808645015537 4 31 2.2626781420190554 4 32 3.230107883198876 4 33 1.4751048389160033 4 34 2.6779258188417145 4 35 2.4751197788769943 4 36 3.931603783853017 4 37 3.774609293244012 4 38 4.180009157803639 4 39 6.635556886463657 4 40 6.454443355846532 4 41 3.3602131371764727 4 42 5.34563661993246 4 43 4.073345100396949 4 44 8.46577590166607 4 45 6.0122330033915325 4 46 8.088029015386855 4 47 8.588883802052301 4 48 7.64275586491841 4 49 5.3884416277530605 4 50 8.316610744790452 4 51 6.716565199334969 4 52 6.638037392861289 4 53 6.077771852175122 4 54 1.0536717346493418 4 55 5.749664685944008 4 56 5.397054469978492 5 1 2.9034233202827338 5 2 3.4439849415641888 5 3 2.9640215190605232 5 4 5.971109157725998 5 5 3.22244154609092 5 6 7.362818490176682 5 7 4.365468460491918 5 8 4.4793025835709415 5 9 4.312600895543909 5 10 3.9102211088810606 5 11 5.186248812208248 5 12 7.934480890209613 5 13 7.864661986520069 5 14 7.557371758749209 5 15 7.699080509836147 5 16 6.62528916565212 5 17 6.913465504889146 5 18 6.283883703926925 5 19 5.471727624002817 5 20 4.961806374512413 5 21 3.317209540495334 5 22 3.89629089325571 5 23 3.409303917543717 5 24 3.6154126041699968 5 25 3.9755468462185135 5 26 2.6550967590135883 5 27 2.9864816674905157 5 28 2.9135208206744707 5 29 2.7071664697263245 5 30 2.9506018521558772 5 31 3.6503324630067127 5 32 4.115046154803814 5 33 3.148685085829584 5 34 3.6068147077305994 5 35 3.42129261838317 5 36 3.3167889690382033 5 37 2.626461145095867 5 38 4.896552367680179 5 39 6.102717380290823 5 40 4.980369281772457 5 41 1.4955217791517796 5 42 7.605389706352213 5 43 11.495073495458676 5 44 11.032195654752492 5 45 8.508529299687833 5 46 9.574942595633768 5 47 10.135550468718971 5 48 9.740039815535692 5 49 10.962762615407382 5 50 10.497351485531194 5 51 8.852367668470773 5 52 10.026185541009436 5 53 10.098512592915867 5 54 9.89614087045181 5 55 10.339788142734132 5 56 8.031622371213057 6 1 2.6964667985436037 6 2 3.0921008835931687 6 3 2.0863403596402392 6 4 5.895746838885415 6 5 3.100122705511211 6 6 7.125717040901318 6 7 4.352714837303509 6 8 4.268867800962241 6 9 4.132890750616369 6 10 3.481815311779606 6 11 5.101611131048827 6 12 7.747814223542946 6 13 7.479154740143251 6 14 9.015922483386891 6 15 7.848066017082527 6 16 6.0415210497100915 6 17 6.596364055613783 6 18 6.0346083416080845 6 19 4.969698638495572 6 20 4.444704925237055 6 21 3.357209540495333 6 22 3.758319878762954 6 23 2.620898120442263 6 24 3.130195212865651 6 25 3.56569177375475 6 26 2.429009802491848 6 27 3.290829493577469 6 28 2.0520715453121525 6 29 2.8422389334944427 6 30 2.75987721447472 6 31 2.612651303586418 6 32 4.704611372195117 6 33 3.1423082742353827 6 34 2.8856552874407484 6 35 3.0392636328759224 6 36 2.7979483893280594 6 37 2.2061712900234056 6 38 3.5782914981149645 6 39 6.332282597682127 6 40 2.88761565858405 6 41 1.6398696052387365 6 42 5.721911445482647 6 43 10.366957553429692 6 44 8.92900724895539 6 45 5.6563553866443534 6 46 9.20450781302507 6 47 9.154101193356649 6 48 9.665836916984968 6 49 10.912327832798685 6 50 9.46314858698047 6 51 8.678454624992515 6 52 9.32241742506741 6 53 9.340251723350647 6 54 10.406865508132974 6 55 8.394860606502249 6 56 7.733071646575379 7 1 2.0570551610787504 7 2 2.7319451405691595 7 3 2.0921807230406273 7 4 5.608920102999626 7 5 3.218660451563558 7 6 6.977544858335886 7 7 3.726065475417286 7 8 3.8520389019789008 7 9 3.9364814925588334 7 10 3.326937526791504 7 11 3.4481393594719307 7 12 6.115973427523045 7 13 6.902472931793696 7 14 7.415879221435777 7 15 6.6947024003834095 7 16 6.465488170627239 7 17 5.950679435237404 7 18 5.140600121837373 7 19 4.1306330966396345 7 20 4.6447914491392766 7 21 3.227856306664492 7 22 3.3436540773353087 7 23 2.7048263056034187 7 24 3.421780763373981 7 25 2.333158786517025 7 26 1.8570868087648265 7 27 1.9367304237094203 7 28 1.8948143530127766 7 29 3.04686796226364 7 30 2.8777660312603572 7 31 2.6881434082803395 7 32 4.7007177965948586 7 33 2.6274910559788367 7 34 2.6911928171833424 7 35 2.2897503298259565 7 36 0.5695252874461652 7 37 0.4622820406182555 7 38 2.0963533627050595 7 39 3.0696825544201722 7 40 -2.1469939023071447 7 41 -9.520199613883046 7 42 4.392255377994005 7 43 9.458456580035794 7 44 7.706424510473884 7 45 5.905544225060964 7 46 8.996634137922324 7 47 9.360028080659266 7 48 9.409293546878981 7 49 11.102464107944694 7 50 8.181729594983928 7 51 10.016546772948384 7 52 10.40310096389501 7 53 10.544880752119845 7 54 11.084996591844849 7 55 10.355708540744086 7 56 8.273413415989179 8 1 2.5551128179996283 8 2 2.6847155351714918 8 3 2.513975856960066 8 4 5.6277301622922025 8 5 2.7729438291959454 8 6 7.096334471911842 8 7 4.133139693368626 8 8 4.212087971698793 8 9 4.041368018831577 8 10 3.423645766415305 8 11 4.944970273395462 8 12 7.715850753223307 8 13 8.482926826702716 8 14 8.048878608064282 8 15 7.198075943626105 8 16 6.907115649670381 8 17 6.288442673838915 8 18 6.209911101187203 8 19 5.5099011399845566 8 20 5.337696785471316 8 21 3.6905428738286687 8 22 3.875651623849315 8 23 3.5079340545300184 8 24 4.019613517412012 8 25 3.6363687640267344 8 26 3.0952794074154113 8 27 2.968216827307863 8 28 2.9571737887110032 8 29 3.425522634109889 8 30 3.1736155507860104 8 31 4.022021960723606 8 32 5.109018757543538 8 33 3.210602894048762 8 34 3.9412439314748937 8 35 3.867137367241618 8 36 0.641903124289346 8 37 0.32509128208216964 8 38 0.9971916370865728 8 39 2.3485621291492684 8 40 -1.7611832296430663 8 41 -10.614249910345936 8 42 3.4092253227905687 8 43 7.1168086552760315 8 44 5.9129262483597955 8 45 3.952364916126186 8 46 6.243618394720528 8 47 6.200755948171025 8 48 6.890176801837065 8 49 8.823584533215598 8 50 6.596164270919321 8 51 7.920860819155703 8 52 7.968833942835921 8 53 8.608466930815403 8 54 8.271665984607065 8 55 8.233852069674771 8 56 6.535731960254154 9 1 2.7399450594131665 9 2 2.5703617531583873 9 3 1.4051809393503802 9 4 5.446471476566579 9 5 3.0131661837720785 9 6 6.864847475683927 9 7 3.9469177358542353 9 8 3.90654896038253 9 9 3.727093649167095 9 10 3.0905109639535198 9 11 4.768277797715491 9 12 7.226075093108157 9 13 7.435676479273688 9 14 7.65360364280718 9 15 7.3553123938941205 9 16 6.650216701883998 9 17 6.277523475903635 9 18 5.599825732912436 9 19 4.998684145741947 9 20 4.53166144697618 9 21 2.9803979462924346 9 22 3.9177401686180247 9 23 3.2730720334857466 9 24 3.7823691259091277 9 25 3.0584453969431564 9 26 2.631908353216481 9 27 2.580684566041235 9 28 1.7042454583556292 9 29 2.5233983537842946 9 30 2.4555293883877596 9 31 3.685115071702363 9 32 4.574176589586422 9 33 3.1568010278585703 9 34 3.5958002149769825 9 35 3.2856404444701255 9 36 1.1457744762845827 9 37 1.4525481016176087 9 38 2.4043784546366993 9 39 4.1728623078270495 9 40 -2.851514776198556 9 41 -6.041289815051117 9 42 5.504520141134822 9 43 9.598841611400708 9 44 8.407268118520605 9 45 6.018674227224061 9 46 9.610304914474352 9 47 9.588883802052305 9 48 9.723807931477726 9 49 11.448559716856654 9 50 9.796481920313798 9 51 10.156715494557726 9 52 10.336910178690598 9 53 9.42720824508978 9 54 10.305416232770654 9 55 9.974570751429784 9 56 10.35626005237248 10 1 2.728696952123528 10 2 3.1200048420616966 10 3 2.4802404245331644 10 4 5.832800700014552 10 5 2.755973884399374 10 6 7.067097097141858 10 7 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-6.432147417836475 114 55 -4.71246410951812 114 56 -1.0981073585166712 115 1 1.116756653616065 115 2 1.3573182748975228 115 3 1.3406881857271955 115 4 3.6044424910593307 115 5 1.4791082127575876 115 6 3.9894851568433474 115 7 2.845468460491915 115 8 2.4326359169042746 115 9 2.5126008955439048 115 10 0.77022110888106 115 11 -0.4570845211250898 115 12 -3.535519109790389 115 13 -1.7770046801466002 115 14 -9.082628241250788 115 15 -2.6142528234971856 115 16 -10.936377501014547 115 17 -12.29820116177752 115 18 -4.066116296073073 115 19 -2.4466057093305125 115 20 -4.583193625487585 115 21 -0.8844571261713341 115 22 -0.8503757734109598 115 23 -1.0406960824562859 115 24 -0.08792072916333638 115 25 -1.019453153781484 115 26 0.11343009234691692 115 27 0.77814833415718 115 28 1.0201874873411398 115 29 1.2621664697263242 115 30 0.16060185215587808 115 31 -1.2413342036599602 115 32 -6.664953845196187 115 33 -1.2363149141704142 115 34 -0.6498519589360612 115 35 -2.0270407149501644 115 36 0.3751223023715369 115 37 0.0014611450958668115 115 38 1.0232190343468481 115 39 3.9293840469574874 115 40 5.522035948439125 115 41 5.127188445818447 115 42 1.390389706352213 115 43 1.7517401621253441 115 44 0.11052898808582512 115 45 -0.9164707003121713 115 46 -5.256724071032899 115 47 -10.119449531281031 115 48 -7.114960184464305 115 49 -9.93223738459262 115 50 -4.284315181135476 115 51 -2.9976323315292284 115 52 -2.4804811256572314 115 53 -3.38982074041747 115 54 -2.360525796214855 115 55 -2.8685451905991997 115 56 0.08162237121305793 116 1 1.5313907999575278 116 2 2.071952421238983 116 3 0.9760540393857298 116 4 2.870296149595916 116 5 1.2754496761722223 116 6 3.3943632056238364 116 7 2.967419680004113 116 8 2.6838554290993955 116 9 2.4443082126170808 116 10 1.743391840588373 116 11 1.1270618203383265 116 12 0.6827735731364406 116 13 -4.738590046000258 116 14 -6.980189216860545 116 15 -2.465472335692308 116 16 -8.771133598575524 116 17 -9.492713356899472 116 18 -2.6136772716828283 116 19 -3.860630099574415 116 20 -3.4899009425607552 116 21 2.739323361633545 116 22 2.7349900802475773 116 23 2.321499039494938 116 24 2.410859758641543 116 25 2.416522455974615 116 26 2.3445276533225297 116 27 1.9214410170840068 116 28 2.990919194658211 116 29 1.4993615916775482 116 30 1.94474819361929 116 31 0.6812267719497953 116 32 -5.652758723244968 116 33 1.120392402902759 116 34 1.1440504800883247 116 35 0.4284470899278858 116 36 3.3525613267617835 116 37 2.7240221207056194 116 38 3.8939507416639216 116 39 7.275725510372123 116 40 8.41288960697571 116 41 5.221700640940398 116 42 3.0336823892790434 116 43 -10.56411349641124 116 44 0.8611387441833855 116 45 3.1621878362731977 116 46 -3.9170899246914352 116 47 -5.622498311768837 116 48 -7.37410652592772 116 49 -3.542603238251157 116 50 -3.194681034794012 116 51 -0.4403152583584955 116 52 -1.1243835646816187 116 53 -1.2465280574906394 116 54 -5.469062381580709 116 55 -2.183789093038225 116 56 0.4121101760911081 117 1 0.636756653616068 117 2 0.4573182748975171 117 3 0.3506881857271935 117 4 2.664442491059326 117 5 1.0091082127575888 117 6 3.3094851568433477 117 7 1.6454684604919123 117 8 1.3326359169042732 117 9 1.312600895543909 117 10 1.27022110888106 117 11 -8.387084521125086 117 12 -11.06551910979039 117 13 -9.0420046801466 117 14 -12.32262824125079 117 15 -5.474252823497185 117 16 -11.681377501014548 117 17 -9.433201161777522 117 18 -3.636116296073073 117 19 -4.021605709330515 117 20 -3.9181936254875858 117 21 -0.5094571261713341 117 22 0.9096242265890382 117 23 0.649303917543719 117 24 -2.037920729163332 117 25 0.7755468462185178 117 26 0.6684300923469166 117 27 1.7331483341571783 117 28 -0.5798125126588616 117 29 0.6271664697263262 117 30 -0.5693981478441259 117 31 -4.536334203659958 117 32 -18.004953845196187 117 33 -3.9313149141704145 117 34 -3.7398519589360646 117 35 -4.552040714950163 117 36 -1.7898776976284623 117 37 -2.093538854904132 117 38 -2.716780965653154 117 39 0.36938404695748517 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1.3075189634311428 118 23 1.546146022806873 118 24 -0.0326575712685937 118 25 1.0923889514816736 118 26 1.1147458818206033 118 27 1.4952535973150773 118 28 -0.39033882844833556 118 29 0.9871664697263256 118 30 1.5474439574190342 118 31 -0.7058078878704848 118 32 -9.480743318880396 118 33 0.783421927934846 118 34 0.373832251590251 118 35 0.1742750745235213 118 36 1.3185433550031185 118 37 1.7843558819379695 118 38 1.98637692908369 118 39 4.366226152220644 118 40 6.415457001070703 118 41 7.581135814239499 118 42 3.0022318116153706 118 43 2.301740162125345 118 44 4.622371093348981 118 45 2.3611608786352 118 46 -6.305408281559213 118 47 -10.42866005759682 118 48 -10.0820654476222 118 49 -8.299342647750516 118 50 -7.898788865346001 118 51 -7.463421805213436 118 52 -6.459428494078281 118 53 -7.962189161470102 118 54 -10.994736322530644 118 55 -9.188282032704464 118 56 -5.605219734050099 119 1 1.5683695568418727 119 2 2.0766731136071996 119 3 2.3584301212110645 119 4 4.988313458801265 119 5 2.06459208372533 119 6 4.879807737488509 119 7 2.6196620088790112 119 8 2.7003778523881437 119 9 2.6803428310277795 119 10 1.7347372379133148 119 11 -10.700632908221863 119 12 -10.424228787209746 119 13 -1.5568433898240208 119 14 -3.689079854154013 119 15 -5.99812379123912 119 16 -10.516216210691969 119 17 -8.208685032745262 119 18 -2.387084038008556 119 19 -1.84096054804019 119 20 -2.4678710448424255 119 21 1.013123518989957 119 22 0.9560758394922644 119 23 0.7125297239953312 119 24 -5.702436858195593 119 25 0.3071597494443239 119 26 1.6529462213791746 119 27 1.4595999470604042 119 28 0.562122971212105 119 29 1.0194245342424537 119 30 2.5364083037687806 119 31 -1.9324632359180214 119 32 -10.003663522615543 119 33 -0.2564762044929978 119 34 -0.13211002345219214 119 35 0.3898947689208043 119 36 2.7727029475328244 119 37 2.432912757999091 119 38 3.626444840798463 119 39 5.77454533728007 119 40 7.788971432310092 119 41 5.6796078006571555 119 42 1.765389706352213 119 43 -2.682130805616591 119 44 -6.372535528043208 119 45 0.3407873642039583 119 46 -7.267207942000638 119 47 -11.712191466764901 119 48 -11.094798894141725 119 49 -7.118527707173266 119 50 -7.125444213393539 119 51 -5.792793621851807 119 52 -6.074029512754006 119 53 -7.966433643643276 119 54 -7.746009667182596 119 55 -8.82580325511533 119 56 -3.819990532012749 120 1 0.8137263505857604 120 2 1.263378880958129 120 3 0.6028093978484037 120 4 3.3226243092411494 120 5 1.5230476066969771 120 6 4.347060914419107 120 7 1.633347248370704 120 8 0.6114237956921471 120 9 0.7732069561499699 120 10 -0.5115970729371213 120 11 -11.991933005973571 120 12 -5.312791837063116 120 13 -9.929277407419328 120 14 -8.064446423068972 120 15 -9.271828581072942 120 16 -12.918953258590307 120 17 -11.727746616322975 120 18 -5.2130859930427675 120 19 -4.724636012360818 120 20 -7.667284534578496 120 21 -3.400366217080421 120 22 -3.062496985532171 120 23 -3.2513021430623468 120 24 -9.728829820072423 120 25 -2.7808167901451206 120 26 -1.6539941500773239 120 27 -1.1044274234185778 120 28 -1.3010246338709877 120 29 -1.2249547423948854 120 30 -2.113640572086549 120 31 -5.113303900629653 120 32 -14.589196269438613 120 33 -4.9749512778067775 120 34 -16.251367110451216 120 35 -4.836889199798648 120 36 -4.004423152173917 120 37 -3.8886903700556488 120 38 -4.488902177774364 120 39 -0.41607049849706 120 40 -3.4681155667123917 120 41 -1.0887206450906426 120 42 -4.931579990617482 120 43 -2.8952295348443506 120 44 -8.659925557368721 120 45 -9.539955548797018 120 46 -14.240814980123806 120 47 -13.108085894917394 120 48 -11.103596548100668 120 49 -15.229964657319893 120 50 -11.09416366598396 120 51 -10.783995967892864 120 52 -10.395632640808746 120 53 -9.969366194962925 120 54 -12.41658640227546 120 55 -11.551120948174958 120 56 -7.762317022726336 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 677.0 5 77.0 6 57.0 7 35.0 8 71.0 9 70.0 10 58.0 11 59.0 12 59.0 13 82.0 14 116.0 15 184.0 16 283.0 17 372.0 18 479.0 19 597.0 20 627.0 21 797.0 22 1010.0 23 1209.0 24 1298.0 25 1381.0 26 1571.0 27 1539.0 28 1663.0 29 1649.0 30 1952.0 31 2299.0 32 2740.0 33 3357.0 34 3917.0 35 4723.0 36 5191.0 37 4730.0 38 2636.0 39 643.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 100.0 0.0 0.0 7 99.35902754729506 0.5642217059409227 0.047709923664122134 0.02904082309990043 8 99.47104215068039 0.35471291072021244 0.11616329239960171 0.05808164619980086 9 98.99379680919483 0.5663782909068277 0.1659716603389971 0.2738532395593452 10 59.95063060073017 28.804347826086957 5.007467640225689 6.237553932957185 11 38.493611018918024 25.172170594092268 19.967640225688683 16.366578161301028 12 38.443826750746766 11.688931297709923 21.22676734152008 28.64047461002323 13 25.74054098904746 14.319200132758048 24.211749087288418 35.728509790906074 14 25.198124559146923 13.138458985104354 28.94900626530019 32.714410190448525 15 25.982500987177087 18.615042500571523 25.11170688114388 30.29074963110751 16 32.099354651283434 16.092216388952295 26.40534539644332 25.403083563320948 17 32.75024900398406 19.01145418326693 23.622177954847277 24.616118857901725 18 33.77102910365715 20.926421474059783 22.55274130313025 22.74980811915282 19 28.690565098332087 26.83387270765912 20.41531823085221 24.060243963156587 20 29.945003631835633 23.285254747328008 20.869565217391305 25.90017640344506 21 29.01479161048068 22.533867186689623 26.234881646370557 22.216459556459142 22 27.246136292915672 20.240638937869516 27.67555232859662 24.837672440618196 23 28.30028211085297 24.58098240955858 24.070693660803187 23.048041818785265 24 28.516013939595087 22.842681712578823 24.402588781944907 24.23871556588118 25 28.020245602389643 25.46257882509127 20.438516428808494 26.078659143710585 26 26.83205551746348 21.043445739574892 26.406116894180226 25.7183818487814 27 27.07749766573296 17.642909015458034 24.629110903620706 30.650482415188296 28 22.447580058602274 26.76170486897612 24.97869952827248 25.812015544149126 29 27.77224435590969 24.595368525896415 23.844206507304115 23.788180610889775 30 25.36257443409341 19.60080502935867 31.887876838807394 23.148743697740525 31 27.798681994885975 19.975885079932645 30.191464149845125 22.03396877533626 32 33.30843758428249 17.52245804028962 25.68618908321404 23.482915292213853 33 30.69171151328776 21.183535229704738 26.517339539136035 21.607413717871466 34 25.626012208795316 19.899505834475313 30.88742161870354 23.58706033802583 35 28.026733639137387 22.698686149567237 31.142198883330913 18.132381327964467 36 27.794743949309236 25.548976835982135 27.445725563519268 19.210553651189365 37 28.856828056462174 24.02968629815188 26.911003471716942 20.202482173669 38 26.56003323639385 25.616950560864147 29.36227669297881 18.46073950976319 39 26.130873042319223 21.122125958013996 23.142285904698433 29.60471509496834 40 16.122504328959174 14.582855235432794 19.76926126050946 49.52537917509858 41 19.648050888242555 16.4047624029629 22.675817622564917 41.271369086229626 42 21.919943469947626 21.984371103167348 27.826502618671544 28.269182808213483 43 13.524940122878268 13.741539102363845 22.797042590857025 49.93647818390086 44 15.086117019058342 13.109387380624712 21.333249927019477 50.471245673297474 45 24.710280373831775 22.201453790238837 29.470404984423677 23.61786085150571 46 26.309343408133184 22.51261079858012 27.96794885101613 23.210096942270567 47 19.92197227525525 19.336764339669628 30.11538142276085 30.625881962314267 48 21.501940196302215 21.611919238031998 34.37571330746405 22.51042725820174 49 16.633704590402832 13.92942645987301 21.107525762464867 48.32934318725929 50 23.006568554086638 19.464538122557578 35.008730356697434 22.520162966658354 51 25.08064683968449 20.4582821702845 31.52406917937938 22.937001810651626 52 22.14886704431856 23.181856047984006 30.60646451182939 24.062812395868043 53 23.87197339706952 20.315909799438845 33.60906162319443 22.203055180297206 54 19.118746883829154 18.445653980388897 31.8638856573043 30.571713478477648 55 21.76730853118963 20.174071996842606 30.946594379011653 27.11202509295611 56 25.068051948051952 19.848311688311686 29.082597402597404 26.001038961038965 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 1.0 12 4.5 13 25.5 14 43.0 15 56.0 16 69.0 17 85.0 18 101.0 19 126.5 20 152.0 21 167.0 22 206.5 23 231.0 24 267.0 25 303.0 26 399.0 27 495.0 28 569.5 29 729.0 30 814.0 31 861.0 32 908.0 33 885.0 34 862.0 35 828.5 36 795.0 37 750.0 38 691.0 39 677.0 40 719.0 41 761.0 42 796.5 43 832.0 44 875.5 45 919.0 46 1028.5 47 1424.5 48 1711.0 49 1825.5 50 1940.0 51 1945.0 52 1950.0 53 1939.5 54 2095.0 55 2261.0 56 2520.5 57 2780.0 58 2961.5 59 3143.0 60 3474.5 61 3806.0 62 3964.0 63 3800.0 64 3478.0 65 3112.0 66 2746.0 67 2503.5 68 2261.0 69 2007.5 70 1754.0 71 1556.0 72 1128.5 73 899.0 74 763.0 75 627.0 76 528.5 77 430.0 78 315.5 79 147.5 80 94.0 81 74.5 82 55.0 83 35.5 84 16.0 85 10.0 86 4.0 87 3.0 88 1.0 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.014520411549950214 10 0.0 11 0.0 12 0.0 13 0.0 14 0.01244606704281447 15 0.1887653501493528 16 0.03526385662130767 17 0.033189512114171926 18 0.0020743445071357454 19 0.008297378028542982 20 0.047709923664122134 21 0.010371722535678725 22 0.006223033521407235 23 0.0 24 0.0 25 0.0 26 0.16387321606372388 27 0.026966478592764687 28 0.1804679721208098 29 0.033189512114171926 30 0.022817789578493196 31 0.21780617324925325 32 0.014520411549950214 33 0.16802190507799536 34 0.09541984732824427 35 0.06015599070693661 36 0.1514271490209094 37 0.21780617324925325 38 0.14105542648523067 39 0.39827414537006306 40 0.5704447394623299 41 0.8650016594756057 42 0.19083969465648853 43 0.4003484898771988 44 0.5185861267839362 45 0.12031198141387323 46 0.07260205774975108 47 0.0414868901427149 48 0.03526385662130767 49 0.3588615997344839 50 0.2074344507135745 51 0.3298207766345835 52 0.39827414537006306 53 0.1929140391636243 54 0.14935280451377364 55 0.13898108197809492 56 0.17217059409226684 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 48208.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.94216727514106 #Duplication Level Percentage of deduplicated Percentage of total 1 93.13074750790472 80.03858280783273 2 4.578697110859019 7.870063060073017 3 1.0933841809273248 2.8190341851974776 4 0.4803166710916946 1.6511782276800528 5 0.18102387101445777 0.7778791901759045 6 0.1424054451980401 0.7343179555260538 7 0.07482320001930921 0.4501327580484567 8 0.05310033549757427 0.36508463325589113 9 0.03620477420289155 0.2800365084633256 >10 0.20516038714971882 3.435114503816794 >50 0.01930921290820883 1.0620643876535016 >100 0.004827303227052208 0.5165117822768005 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 131 0.2717391304347826 No Hit GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 118 0.2447726518420179 No Hit GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 91 0.1887653501493528 No Hit GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTCCTCTTCC 73 0.1514271490209094 No Hit GGTACAGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG 68 0.14105542648523067 No Hit GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGACCACCCGACTGCTCTTC 64 0.1327580484566877 No Hit GGTACAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG 59 0.12238632592100895 No Hit GGTACAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG 55 0.11408894789246599 No Hit GGTACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTT 51 0.105791569863923 No Hit GGTACAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG 51 0.105791569863923 No Hit GGTACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGCCCACCCGACTGCTCTT 50 0.10371722535678725 No Hit GGTACAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 49 0.10164288084965152 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.002074344507135745 0.0 0.0 0.0 0.0 7 0.002074344507135745 0.0 0.0 0.0 0.0 8 0.002074344507135745 0.0 0.0 0.0 0.0 9 0.00414868901427149 0.0 0.0 0.0 0.0 10 0.00414868901427149 0.0 0.0 0.0 0.0 11 0.00829737802854298 0.0 0.0 0.0 0.0 12 0.01244606704281447 0.0 0.0 0.0 0.0 13 0.01659475605708596 0.0 0.0 0.0 0.0 14 0.018669100564221705 0.0 0.0 0.0 0.0 15 0.02074344507135745 0.0 0.0 0.0 0.0 16 0.02074344507135745 0.0 0.0 0.0 0.0 17 0.02074344507135745 0.0 0.0 0.0 0.0 18 0.022817789578493196 0.0 0.0 0.0 0.0 19 0.022817789578493196 0.0 0.0 0.0 0.0 20 0.022817789578493196 0.0 0.0 0.0 0.0 21 0.02489213408562894 0.0 0.0 0.0 0.0 22 0.026966478592764687 0.0 0.0 0.0 0.0 23 0.026966478592764687 0.0 0.0 0.0 0.0 24 0.026966478592764687 0.0 0.0 0.0 0.0 25 0.026966478592764687 0.0 0.0 0.0 0.0 26 0.031115167607036177 0.0 0.0 0.0 0.0 27 0.031115167607036177 0.0 0.0 0.0 0.0 28 0.03318951211417192 0.0 0.0 0.0 0.0 29 0.03318951211417192 0.0 0.0 0.0 0.0 30 0.03318951211417192 0.0 0.0 0.0 0.0 31 0.03318951211417192 0.0 0.0 0.0 0.0 32 0.03526385662130767 0.0 0.0 0.0 0.0 33 0.03526385662130767 0.0 0.0 0.0 0.0 34 0.03526385662130767 0.0 0.0 0.0 0.0 35 0.03526385662130767 0.0 0.0 0.0 0.0 36 0.03526385662130767 0.0 0.0 0.0 0.0 37 0.03526385662130767 0.0 0.0 0.0 0.0 38 0.03526385662130767 0.0 0.0 0.0 0.0 39 0.03733820112844341 0.0 0.0 0.0 0.0 40 0.03733820112844341 0.0 0.0 0.0 0.0 41 0.03941254563557916 0.0 0.0 0.0 0.0 42 0.03941254563557916 0.0 0.0 0.0 0.0 43 0.03941254563557916 0.0 0.0 0.0 0.0 44 0.0414868901427149 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGCCCA 40 2.3192115E-9 50.624737 39 TACGCCC 40 2.3192115E-9 50.624737 38 CTGTCTC 15 0.007979994 50.624737 40 CGGACAA 15 0.008013442 50.57128 37 TGTCTCA 20 3.9172094E-4 50.571278 41 GTACGCC 45 1.1641532E-10 50.571278 37 TATGCCT 15 0.008148281 50.35857 35 ATGCCTG 15 0.008148281 50.35857 36 GTAAGCA 15 0.008148281 50.35857 35 GGTAAGC 20 4.0205452E-4 50.30567 34 CCTGAAC 15 0.008250511 50.20021 43 CCAAGAC 15 0.008250511 50.20021 43 ACAGTTC 85 0.0 50.20021 43 CTGAACG 15 0.0082848 50.147648 44 CATATGC 45 1.2732926E-10 50.147644 33 CAACACA 20 4.0835785E-4 50.14764 44 TCAAAGA 35 5.0293238E-8 50.14764 45 AAAGATT 35 5.0293238E-8 50.14764 47 GTGGCAA 15 0.008423013 49.93848 30 CGATGGA 15 0.008423013 49.93848 13 >>END_MODULE