FastQCFastQC Report
Fri 17 Jun 2016
SRR1526311_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526311_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22737
Sequences flagged as poor quality0
Sequence length56
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC840.3694418788758412No Hit
CATCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC830.36504376127017635No Hit
CATCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGACCACCCGACTGCTCTTC760.3342569380305229No Hit
CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC760.3342569380305229No Hit
CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC720.3166644676078639No Hit
CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC620.27268329155121607No Hit
CATCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACCGCTCTTC400.175924704226591No Hit
CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTCCCTACCAAGCGC370.16273035140959669No Hit
CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTCCTCTTCC360.15833223380393194No Hit
CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGTTCTTCC340.14953599859260236No Hit
CATCCCGGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGGTCTG230.10115670493028985No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACCAA150.007881816550.69438640
CCCCGAC203.8259174E-450.69438641
GCTTGCC150.007881816550.69438640
AAAAACC150.007881816550.69438638
CTCGCCT150.007881816550.69438641
CTTGCCT251.8631874E-550.69437841
CCCCCGA251.8631874E-550.69437840
GGACTGG150.00802325350.46756437
GCGGTCC150.00802325350.46756437
ACCAAGC203.9119113E-450.46756448
TAGCAAG354.630965E-850.4675647
CTAGCAA354.630965E-850.4675646
AGCAAGC354.630965E-850.4675648
CAAGCAT251.9135636E-550.4675649
TAGGACT203.9554804E-450.35491635
TCTGCGA150.00809467550.3549145
CCTAGCA354.713911E-850.35490845
ATTTCAA251.9391717E-550.35490845
AATTTCA150.00816656850.24276444
GTAAGAC203.999434E-450.24276434