##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526311_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 22737 Sequences flagged as poor quality 0 Sequence length 56 %GC 60 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.257069974051106 39.0 38.0 40.0 35.0 40.0 2 36.96657430619695 39.0 38.0 39.0 33.0 40.0 3 37.832255794519945 39.0 38.0 40.0 35.0 40.0 4 37.74314993182918 39.0 38.0 40.0 35.0 40.0 5 37.99947222588732 39.0 38.0 40.0 35.0 40.0 6 37.87891982231605 39.0 38.0 40.0 35.0 40.0 7 37.49540396710208 39.0 38.0 39.0 35.0 40.0 8 37.5620354488279 39.0 38.0 39.0 35.0 40.0 9 37.71825658618111 39.0 38.0 39.0 35.0 40.0 10 37.0489950301271 39.0 37.0 39.0 33.0 40.0 11 35.62075031886353 38.0 35.0 39.0 29.0 40.0 12 32.965430795619476 36.0 31.0 39.0 18.0 40.0 13 32.53723006553195 36.0 30.0 39.0 16.0 40.0 14 31.730791221357258 36.0 28.0 39.0 14.0 40.0 15 32.771517790385715 37.0 30.0 39.0 17.0 40.0 16 32.426705370101594 36.0 29.0 39.0 18.0 40.0 17 32.41711747372125 36.0 30.0 39.0 18.0 40.0 18 32.69855301930774 36.0 30.0 39.0 20.0 40.0 19 33.658046356159566 36.0 31.0 39.0 24.0 40.0 20 33.59154681796191 36.0 31.0 39.0 24.0 40.0 21 35.13634164577561 38.0 35.0 39.0 27.0 40.0 22 34.814531380569115 38.0 34.0 39.0 26.0 40.0 23 35.01926375511281 38.0 34.0 39.0 27.0 40.0 24 34.641289528081984 38.0 33.0 39.0 25.0 40.0 25 34.941417073492545 38.0 34.0 39.0 27.0 40.0 26 35.086027180366806 38.0 35.0 39.0 27.0 40.0 27 35.08039758983155 38.0 35.0 39.0 27.0 40.0 28 34.904428904428904 38.0 35.0 39.0 27.0 40.0 29 34.76258961164621 38.0 34.0 39.0 27.0 40.0 30 34.522320446848745 38.0 34.0 39.0 26.0 40.0 31 34.08114526982452 38.0 33.0 39.0 24.0 40.0 32 33.043717289000305 37.0 32.0 39.0 18.0 40.0 33 34.25531072700884 38.0 33.0 39.0 26.0 40.0 34 33.781457536174514 37.0 33.0 39.0 23.0 40.0 35 33.889563266921755 37.0 33.0 39.0 25.0 40.0 36 32.048291331310196 35.0 31.0 38.0 22.0 39.0 37 31.851827417865152 35.0 31.0 38.0 19.0 39.0 38 31.11034877072613 35.0 30.0 38.0 14.0 39.0 39 29.25808154110041 34.0 27.0 37.0 6.0 39.0 40 25.481725821348462 31.0 13.0 36.0 4.0 38.0 41 22.48212165193297 26.0 10.0 33.0 4.0 37.0 42 25.74702027532216 30.0 21.0 34.0 4.0 37.0 43 22.176892290099836 26.0 9.0 33.0 4.0 36.0 44 22.807450411223996 27.0 10.0 33.0 4.0 36.0 45 25.19351717464925 30.0 20.0 34.0 4.0 37.0 46 24.780973743237894 30.0 15.0 35.0 4.0 38.0 47 24.336763865065752 29.0 10.0 35.0 4.0 38.0 48 24.300787263051415 29.0 11.0 35.0 4.0 38.0 49 21.78809869375907 26.0 5.0 35.0 4.0 38.0 50 24.16088314201522 29.0 9.0 35.0 4.0 38.0 51 25.57830848396886 32.0 4.0 37.0 4.0 39.0 52 25.372828429432204 31.0 4.0 37.0 4.0 39.0 53 25.134406474029117 31.0 4.0 37.0 4.0 39.0 54 24.320402867572678 31.0 4.0 36.0 4.0 39.0 55 24.476448080221665 31.0 4.0 36.0 4.0 39.0 56 23.99159959537318 31.0 4.0 37.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.5736816463857295 1 2 1.8761653221000216 1 3 1.1330983373987493 1 4 1.2321053509870197 1 5 0.9776306785862943 1 6 1.1024455995287497 1 7 1.1893509704955605 1 8 1.2334878998135466 1 9 0.8810592925236307 1 10 1.2943426348396727 1 11 -3.156279419757375 1 12 -5.059934158382095 1 13 -21.770546564406054 1 14 -16.67179591788939 1 15 -10.169662152349915 1 16 -21.60659382237575 1 17 -21.79587439233472 1 18 -20.870785886025555 1 19 -6.1820313872450825 1 20 -10.620182600375674 1 21 -10.543229302182233 1 22 -4.596636239487211 1 23 -5.275895442018889 1 24 -3.756095826491933 1 25 -4.656744911320612 1 26 3.5127786313088123 1 27 3.559118014282994 1 28 3.253015706974466 1 29 3.34266011154628 1 30 3.187820140335802 1 31 2.3622358536768147 1 32 -10.731065548956181 1 33 2.06638325378335 1 34 1.7796964536849842 1 35 2.0501409239378034 1 36 4.661566118589501 1 37 5.9819060514125475 1 38 6.0850898485465095 1 39 8.51561246169473 1 40 10.630599833599767 1 41 -7.150006712062357 1 42 -11.898654976629828 1 43 -14.667470744554496 1 44 -15.79915529074089 1 45 -15.922713962299579 1 46 -17.589127070055973 1 47 -17.719394795036376 1 48 -16.6939384851572 1 49 -14.250334510456579 1 50 -16.008375547327585 1 51 -17.531315546346093 1 52 -17.27751051342787 1 53 -17.119464778301975 1 54 -17.867622640958125 1 55 -17.195295278523872 1 56 -16.462549326102415 2 1 0.17606259876668418 2 2 1.707117703052404 2 3 0.602145956446364 2 4 0.8368672557489205 2 5 0.7800116309672447 2 6 0.8072075042906519 2 7 0.4345890657336611 2 8 1.0096783760040253 2 9 0.566773578237914 2 10 0.8371997776968172 2 11 -2.789612753090708 2 12 -12.419457967905903 2 13 -13.549117992977486 2 14 -15.069414965508436 2 15 -9.919662152349915 2 16 -2.9637366795186075 2 17 -3.7958743923347207 2 18 -8.863643028882699 2 19 -2.982031387245083 2 20 -2.491611171804248 2 21 1.987723078770152 2 22 2.9057447128937426 2 23 2.98362836750492 2 24 3.1391422687461628 2 25 2.536112231536528 2 26 3.0056357741659596 2 27 2.587689442854426 2 28 2.569682373641136 2 29 1.2593267782129445 2 30 1.2378201403357991 2 31 -1.585383193942235 2 32 -9.169160787051421 2 33 -1.1764738890737974 2 34 -0.5107797367911999 2 35 0.4525218763187553 2 36 4.573470880494266 2 37 3.420001289507784 2 38 5.416042229498892 2 39 7.482279128361398 2 40 9.354409357409288 2 41 7.409517097461453 2 42 3.5180116900368397 2 43 -0.12699455407830484 2 44 -9.011060052645652 2 45 1.4439527043670886 2 46 -11.501031831960733 2 47 -12.395585271226851 2 48 -8.90346229468101 2 49 -10.547953558075628 2 50 -9.710756499708536 2 51 -7.374172689203235 2 52 -7.982272418189773 2 53 -9.248036206873401 2 54 -14.70333692667241 2 55 -9.450057183285775 2 56 -9.431596945150034 3 1 0.9881838108878895 3 2 2.628329824264526 3 3 1.5172974715978853 3 4 1.5005036193852845 3 5 1.3618298127854302 3 6 1.6132681103512567 3 7 1.049740580885171 3 8 1.2096783760040282 3 9 1.3031372146015485 3 10 1.5887149292119673 3 11 1.4952357317577736 3 12 1.6987238502759112 3 13 -4.797602841462332 3 14 -6.07547557156904 3 15 -2.5863288190165825 3 16 3.263536047754119 3 17 3.0526104561501306 3 18 2.3121145468748807 3 19 1.333120127906433 3 20 0.8083888281957528 3 21 2.8301473211943886 3 22 2.5905931977422227 3 23 2.6321132159897687 3 24 3.3391422687461585 3 25 2.9936879891122885 3 26 1.9662418347720205 3 27 1.1695076246726046 3 28 1.396955100913857 3 29 1.7290237479099133 3 30 2.5287292312448955 3 31 3.2085561999971617 3 32 0.9247786068879726 3 33 2.559889747289837 3 34 2.6952808692694035 3 35 1.9040370278339083 3 36 4.27953148655487 3 37 4.159395228901726 3 38 4.003921017377678 3 39 6.90955185563412 3 40 9.154409357409286 3 41 9.015577703522059 3 42 6.2907389627641095 3 43 -0.378509705593455 3 44 0.158636917051318 3 45 6.834861795276179 3 46 1.4595742286453266 3 47 -3.1349792106207914 3 48 1.193507402288688 3 49 -6.88128689140896 3 50 2.213485924533888 3 51 0.2197667047361591 3 52 1.3025760666587125 3 53 0.5428728840356882 3 54 -0.7518217751572571 3 55 -1.1682390014675939 3 56 4.532039418486331 4 1 0.8988696163105416 4 2 2.419398404806792 4 3 1.4582863073235615 4 4 1.5666918171524316 4 5 1.2238712800900515 4 6 1.173874170957319 4 7 0.8416066095933061 4 8 0.6658187268812199 4 9 1.324668315080018 4 10 1.805620830328401 4 11 2.5279311065584125 4 12 2.9682613303397076 4 13 5.156145164917255 4 14 4.600760473088055 4 15 6.60665363712377 4 16 6.77310542574455 4 17 6.440967712928437 4 18 6.183725392169933 4 19 3.607442296965445 4 20 5.203125670301013 4 21 3.394740622629797 4 22 3.991709625174444 4 23 3.564330121890883 4 24 3.6461598126058092 4 25 3.7624280210102157 4 26 2.589846300481746 4 27 2.9613736533807398 4 28 1.9240683385534112 4 29 2.8031864273357527 4 30 2.832556982441062 4 31 2.6742659288647843 4 32 3.3290848269836637 4 33 3.7129997951367315 4 34 3.396237807068445 4 35 2.559539420178403 4 36 3.787505968213562 4 37 4.869124096525329 4 38 6.312533457569067 4 39 7.778770356431572 4 40 9.540374269689991 4 41 4.979692536057943 4 42 8.351345023370172 4 43 8.492303691535732 4 44 11.33806275437189 4 45 9.472022879805682 4 46 9.653354132951549 4 47 9.42722174631701 4 48 8.37373068777513 4 49 5.443274512099812 4 50 9.482225956431815 4 51 7.115300995007292 4 52 7.887903020406718 4 53 4.978279582600283 4 54 -0.5875474529882005 4 55 4.878012992152822 4 56 4.738578493446457 5 1 1.4531459321000142 5 2 3.059201036385737 5 3 1.981312623113034 5 4 2.116033922415589 5 5 1.857094964300579 5 6 2.017624170957319 5 7 1.774172399066991 5 8 1.9388450426706925 5 9 1.8230235782379154 5 10 2.6246997776968186 5 11 4.343720580242625 5 12 6.7659586987607625 5 13 7.7696320070225156 5 14 7.738918367824898 5 15 7.424087847650085 5 16 8.161263320481392 5 17 7.860375607665279 5 18 7.361356971117303 5 19 5.7867186127549175 5 20 6.089638828195753 5 21 4.639806412103482 5 22 4.751578046227074 5 23 3.366961700838253 5 24 5.016225602079494 5 25 3.7986122315365307 5 26 3.686885774165958 5 27 3.2376894428544247 5 28 3.721765706974466 5 29 3.81141011154628 5 30 3.625320140335802 5 31 3.9604501393910994 5 32 5.728755879615246 5 33 4.517276110926204 5 34 4.54755359654213 5 35 4.273355209652088 5 36 6.527637547160932 5 37 6.459584622841117 5 38 7.643125562832225 5 39 9.48436246169473 5 40 6.643992690742621 5 41 4.711600430794785 5 42 9.413845023370172 5 43 12.783422112588362 5 44 12.334773280687681 5 45 9.671036037700421 5 46 11.2635515013726 5 47 10.575248062106482 5 48 10.631954371985657 5 49 11.441629775257706 5 50 11.48716016695813 5 51 10.263327310796765 5 52 10.52606091514356 5 53 10.264463793126598 5 54 11.28416307332759 5 55 10.077026150047558 5 56 8.9392363881833 6 1 1.5033353260394051 6 2 3.082875278809979 6 3 1.7294186837190964 6 4 2.167170286051956 6 5 1.90728435823997 6 6 1.8253893224724678 6 7 1.867922399066991 6 8 1.9369511032767548 6 9 2.000106911571251 6 10 1.8008361413331855 6 11 3.2831145196365625 6 12 7.425996577548638 6 13 7.263003219143727 6 14 8.379069882976413 6 15 8.140943908256148 6 16 8.354445138663213 6 17 7.688974092513764 6 18 6.615144849905178 6 19 6.302817097603402 6 20 5.899297919104846 6 21 4.466510957558029 6 22 4.5299871371361675 6 23 4.02605260992916 6 24 4.581566511170401 6 25 5.2058092012334996 6 26 3.693514562044747 6 27 3.9270833822483624 6 28 4.275742979701739 6 29 3.062357081243249 6 30 3.892365594881255 6 31 3.663101654542615 6 32 4.5005361826455506 6 33 4.6204958078958995 6 34 5.392250566239099 6 35 3.7828249066217836 6 36 5.370440577463963 6 37 5.18969825920475 6 38 6.367557381014045 6 39 7.424703370785636 6 40 3.5180457210456524 6 41 4.076183764128118 6 42 8.078617750642898 6 43 13.01542968834594 6 44 12.431364189778588 6 45 9.68334664376103 6 46 13.126240895311991 6 47 14.046838971197388 6 48 13.587446796228079 6 49 15.724773714651644 6 50 13.849849560897521 6 51 12.886433371402827 6 52 11.120757884840529 6 53 12.754994096156903 6 54 13.581511558176075 6 55 12.2257003924718 6 56 13.653251539698449 7 1 1.2270429909235432 7 2 2.98750985991515 7 3 1.404106740760092 7 4 1.8623574518273571 7 5 1.778050846653521 7 6 1.997403582722022 7 7 1.603216516714049 7 8 1.0234038662001055 7 9 1.5197147547085024 7 10 2.4371997776968186 7 11 3.079014697889683 7 12 4.8119145811137045 7 13 6.0214702423166315 7 14 8.040388956060195 7 15 7.080337847650085 7 16 8.330380967540219 7 17 6.821772666488808 7 18 6.795180500529071 7 19 6.0238509656960915 7 20 5.955447651725166 7 21 4.126938765044656 7 22 4.203783928580016 7 23 3.999314642014724 7 24 4.054828543255965 7 25 4.35375929036006 7 26 2.84681224475419 7 27 3.2523953252073667 7 28 4.355956883445053 7 29 3.269130699781577 7 30 3.614290728571099 7 31 3.6479501393910994 7 32 5.668094114909362 7 33 4.041173169749733 7 34 3.92255359654213 7 35 4.321149327299146 7 36 2.634255194219758 7 37 2.641569916958762 7 38 3.814081445185167 7 39 3.2509065793417875 7 40 1.7818603378014437 7 41 -8.639502510381686 7 42 5.468992082193701 7 43 8.822025053764833 7 44 7.845802692452388 7 45 6.047874272994537 7 46 8.114654442549071 7 47 8.72598335622413 7 48 7.604380842573892 7 49 10.81479153996359 7 50 7.808851343428717 7 51 10.443474369620294 7 52 10.709884444555328 7 53 11.304904969597185 7 54 12.361368955680533 7 55 10.218570267694616 7 56 10.942912858771532 8 1 1.516803339507419 8 2 2.877488073422775 8 3 1.5947385490389578 8 4 2.0998302187118867 8 5 1.9207523717079837 8 6 2.081281578364724 8 7 1.2753298064743959 8 8 1.617085783411433 8 9 2.000106911571251 8 10 2.2890516295486734 8 11 4.232609469131511 8 12 6.84350499505706 8 13 7.495326451466958 8 14 8.271325775232302 8 15 7.450708218020459 8 16 8.017744801962873 8 17 7.68560708914676 8 18 6.514134748895081 8 19 5.929079723866032 8 20 6.030611050417974 8 21 3.439574930622001 8 22 3.2538928610418907 8 23 3.7095542934308483 8 24 4.30210523170912 8 25 3.791667787092088 8 26 3.4241542926844772 8 27 3.4321338872988676 8 28 3.040052744011504 8 29 2.7963638152499826 8 30 3.47485717737284 8 31 3.370172361613321 8 32 5.197505879615246 8 33 3.2198224072225017 8 34 4.459590633579168 8 35 3.3080774318743096 8 36 1.5993968064201916 8 37 1.9237049932114907 8 38 3.179005192461851 8 39 2.774871720953989 8 40 -2.438183235183306 8 41 -11.220112532168178 8 42 4.869863541888691 8 43 8.254486927403175 8 44 7.781532539946941 8 45 6.373582333996719 8 46 10.324894093965195 8 47 10.545155469513887 8 48 9.126167334948619 8 49 12.377972367850298 8 50 10.381836092884058 8 51 12.41841990338936 8 52 10.928838692921339 8 53 13.081593422756224 8 54 13.911477888142407 8 55 13.094387261158673 8 56 13.094328980775895 9 1 1.3593959321000142 9 2 2.402951036385737 9 3 1.075062623113034 9 4 1.522283922415589 9 5 1.763344964300579 9 6 1.736374170957319 9 7 0.8991723990669911 9 8 1.1575950426706925 9 9 1.5417735782379154 9 10 1.2184497776968186 9 11 2.749970580242625 9 12 5.8597086987607625 9 13 5.6446320070225156 9 14 8.020168367824898 9 15 7.299087847650085 9 16 7.5987633204813925 9 17 7.141625607665279 9 18 4.955106971117303 9 19 4.4742186127549175 9 20 4.620888828195753 9 21 3.546056412103482 9 22 3.845328046227074 9 23 2.491961700838253 9 24 3.391225602079494 9 25 3.6111122315365307 9 26 2.905635774165958 9 27 3.4876894428544247 9 28 3.065515706974466 9 29 2.78016011154628 9 30 2.969070140335802 9 31 3.3042001393910994 9 32 3.791255879615246 9 33 2.517276110926204 9 34 3.14130359654213 9 35 2.929605209652088 9 36 2.340137547160932 9 37 2.647084622841117 9 38 3.6431255628322248 9 39 4.1718624616947295 9 40 2.018992690742621 9 41 0.7741004307947854 9 42 7.976345023370172 9 43 10.689672112588362 9 44 8.991023280687681 9 45 8.327286037700421 9 46 9.2635515013726 9 47 9.887748062106482 9 48 9.319454371985657 9 49 13.160379775257706 9 50 9.33091016695813 9 51 9.325827310796765 9 52 9.18231091514356 9 53 7.920713793126598 9 54 7.565413073327591 9 55 8.702026150047558 9 56 9.0954863881833 10 1 1.2093959321000156 10 2 2.3071177030524055 10 3 1.8688126231130369 10 4 1.736867255748919 10 5 1.7466782976339132 10 6 1.9072075042906533 10 7 1.2012557324003268 10 8 1.7096783760040282 10 9 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0.6927568801569706 119 34 0.8071689811575169 119 35 0.7781629019597815 119 36 4.801676008699392 119 37 5.476411545918037 119 38 6.549375562832225 119 39 8.746381692463963 119 40 8.026204229204161 119 41 -3.949457261512908 119 42 -4.5717318997067515 119 43 -7.25263557971933 119 44 -10.093111334696934 119 45 -10.076560116145732 119 46 -12.729236960165862 119 47 -12.169944245585826 119 48 -13.91371870493742 119 49 -11.214620224742294 119 50 -10.7748590638111 119 51 -11.020326535357082 119 52 -12.61816985408721 119 53 -14.471113129950325 119 54 -12.980260003595486 119 55 -12.55518538841398 119 56 -10.4982636118167 120 1 0.10939593210001419 120 2 1.6261653221000216 120 3 0.3830983373987493 120 4 0.6606767795584432 120 5 0.44191639287200957 120 6 0.24530274238588845 120 7 -0.06064902950443951 120 8 0.5192021855278384 120 9 0.4524878639522001 120 10 0.2229140634111033 120 11 -11.44199370547166 120 12 -3.309934158382095 120 13 -6.1634037072632 120 14 -5.850367346460818 120 15 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-17.0480362068734 120 54 -16.189051212386694 120 55 -16.159580992809584 120 56 -15.069692183245271 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 113.0 5 17.0 6 12.0 7 22.0 8 33.0 9 71.0 10 82.0 11 57.0 12 42.0 13 49.0 14 48.0 15 73.0 16 125.0 17 160.0 18 211.0 19 218.0 20 276.0 21 390.0 22 444.0 23 540.0 24 592.0 25 673.0 26 727.0 27 743.0 28 706.0 29 831.0 30 917.0 31 1114.0 32 1426.0 33 1634.0 34 1892.0 35 2211.0 36 2442.0 37 2235.0 38 1280.0 39 331.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 0.0 100.0 7 97.60742402251836 1.842811276773541 0.3034701147908695 0.24629458591722744 8 99.0148216563311 0.5937458767647447 0.175924704226591 0.215507762677574 9 98.2798064232292 0.9018917729872415 0.29916410030796303 0.5191377034755829 10 74.93952588292211 18.304965474776797 2.5465100936799048 4.2089985486211905 11 53.21076706544687 19.74841660802252 14.329697396199858 12.711118930330754 12 44.92677134186568 9.139288384571403 18.573250648722347 27.360689624840568 13 31.569688173461756 11.021682719795928 22.522760258609313 34.885868848133 14 23.011613584374448 11.358437445011438 31.40506774590885 34.224881224705264 15 27.847488222603793 15.757495707304187 29.35323383084577 27.04178223924625 16 40.10822225155074 15.99577669262241 20.892173683515903 23.00382737231094 17 37.72049443540228 17.859499406149652 22.381559846918577 22.038446311529494 18 36.22009148486981 20.01671358198452 20.79081632653061 22.97237860661506 19 35.51097619990322 25.33984426554045 17.579516959218687 21.569662575337645 20 34.68965820613206 25.988650859983288 20.42405313860907 18.89763779527559 21 33.79222378606615 23.65411681914145 23.09113300492611 19.46252638986629 22 25.935699520605183 21.423230857193122 30.742842063596783 21.898227558604916 23 29.405814311474685 20.719532040286754 29.94678277697146 19.927870871267096 24 35.12776531644456 21.18133438888156 19.536438404362933 24.154461890310948 25 31.213440647402912 21.89382944099925 19.589215815630908 27.303514095966925 26 25.268959435626105 20.163139329805997 25.515873015873012 29.052028218694886 27 23.837388358484755 17.440274539135025 26.793963658761932 31.928373443618284 28 21.48399612027158 24.248302618816684 22.36575257913764 31.901948681774094 29 30.04178579283044 26.32504948317572 20.41345942379591 23.219705300197933 30 31.230200633579724 18.052622316085884 26.949137627595913 23.76803942273847 31 32.97023153252481 17.7728776185226 26.981256890848954 22.27563395810364 32 39.472179458983945 15.641082032109082 21.609852650098965 23.276885858808004 33 40.19750473923202 17.947361460124323 22.699819247894897 19.155314552748752 34 29.145750770585643 18.3223249669749 32.351387054161165 20.18053720827829 35 24.566499427867267 28.941114338526535 28.360179561658306 18.13220667194789 36 28.042118248303815 32.822275090316325 20.918142567627104 18.217464093752753 37 32.517312866657846 27.700586652551717 18.618499404525604 21.163601076264833 38 31.970571390810164 28.349266487510462 20.710163443323495 18.969998678355875 39 28.400406414277512 21.906613067102533 18.02800724477625 31.664973273843707 40 19.940686968838527 13.225920679886686 15.483356940509916 51.350035410764875 41 17.599466548121807 17.466103578573016 18.43076239164259 46.50366748166259 42 22.690409026798307 23.430888575458393 23.986248236953458 29.892454160789843 43 14.308405029781602 11.819986763732627 19.71762629605118 54.15398191043459 44 17.776992401484364 13.129528185191731 19.22601166283796 49.86746775048595 45 30.04050008804367 20.0563479485825 23.573692551505545 26.32945941186829 46 25.411423039690224 24.667781395758162 22.322450057203202 27.59834550734841 47 20.314974485307054 19.33397853246525 25.567481963751536 34.78356501847616 48 24.66229594755137 20.341444097329166 30.958771505258063 24.0374884498614 49 20.585641206562002 12.127359322631857 19.67278179573117 47.61421767507497 50 25.066137566137563 17.32804232804233 27.389770723104057 30.216049382716047 51 22.05343342901303 21.02450872157209 30.07727975270479 26.844778096710094 52 21.240775926826036 21.899164862356944 28.390261146215366 28.469798064601655 53 23.535634007668914 18.555247036008637 33.11296222839261 24.796156727929834 54 20.557291895936963 15.622661442972225 30.334111018180216 33.4859356429106 55 24.534120445834617 17.15934622670602 29.247984492708927 29.058548834750432 56 23.899731265694523 20.37094145116525 25.142076743468877 30.587250539671352 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 1.0 10 1.0 11 1.0 12 1.0 13 0.5 14 0.0 15 5.0 16 10.0 17 18.0 18 26.0 19 39.5 20 53.0 21 54.5 22 67.0 23 78.0 24 71.5 25 65.0 26 67.0 27 69.0 28 83.5 29 125.0 30 152.0 31 144.0 32 136.0 33 156.0 34 176.0 35 176.0 36 176.0 37 182.0 38 203.5 39 219.0 40 238.5 41 258.0 42 254.5 43 251.0 44 295.5 45 340.0 46 326.0 47 349.0 48 386.0 49 437.0 50 488.0 51 549.0 52 610.0 53 636.5 54 770.5 55 878.0 56 983.0 57 1088.0 58 1278.0 59 1468.0 60 1656.0 61 1844.0 62 2212.0 63 2544.0 64 2508.0 65 2315.0 66 2122.0 67 1884.0 68 1646.0 69 1433.5 70 1221.0 71 1039.5 72 741.0 73 624.0 74 540.5 75 457.0 76 376.0 77 295.0 78 238.5 79 136.5 80 91.0 81 64.0 82 37.0 83 24.5 84 12.0 85 8.5 86 5.0 87 4.0 88 2.0 89 1.0 90 1.0 91 1.0 92 1.0 93 1.0 94 1.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.03078682323965343 10 0.0 11 0.004398117605664775 12 0.0 13 0.0 14 0.021990588028323876 15 0.1055548225359546 16 0.02638870563398865 17 0.0175924704226591 18 0.004398117605664775 19 0.02638870563398865 20 0.0175924704226591 21 0.004398117605664775 22 0.0 23 0.0 24 0.0 25 0.0 26 0.2506927035228922 27 0.0351849408453182 28 0.24189646831156267 29 0.00879623521132955 30 0.039583058450982984 31 0.27268329155121607 32 0.00879623521132955 33 0.23749835070589786 34 0.11874917535294893 35 0.06597176408497163 36 0.17152658662092624 37 0.29027576197387517 38 0.16712846901526146 39 0.4398117605664775 40 0.6377270528213925 41 1.0643444605708756 42 0.215507762677574 43 0.3166644676078639 44 0.4442098781721423 45 0.0923604697189603 46 0.04837929366231253 47 0.021990588028323876 48 0.04398117605664775 49 0.2682851739455513 50 0.2506927035228922 51 0.4046268197211594 52 0.4662004662004662 53 0.2111096450719092 54 0.0879623521132955 55 0.16712846901526146 56 0.16712846901526146 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 22737.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.34107402031931 #Duplication Level Percentage of deduplicated Percentage of total 1 92.95506081220367 79.32884725337556 2 4.535147392290249 7.740686985970005 3 1.2059369202226344 3.0874785591766725 4 0.468975468975469 1.6009148084619784 5 0.24737167594310452 1.055548225359546 6 0.0979179550608122 0.5013854070457844 7 0.08245722531436817 0.4925891718344549 8 0.08761080189651618 0.5981439943704094 9 0.02576788291074005 0.19791529225491492 >10 0.2628324056895485 3.4041430267845363 >50 0.030921459492888066 1.9923472753661433 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 84 0.3694418788758412 No Hit CATCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 83 0.36504376127017635 No Hit CATCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGACCACCCGACTGCTCTTC 76 0.3342569380305229 No Hit CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC 76 0.3342569380305229 No Hit CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 72 0.3166644676078639 No Hit CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 62 0.27268329155121607 No Hit CATCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACCGCTCTTC 40 0.175924704226591 No Hit CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTCCCTACCAAGCGC 37 0.16273035140959669 No Hit CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTCCTCTTCC 36 0.15833223380393194 No Hit CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGTTCTTCC 34 0.14953599859260236 No Hit CATCCCGGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGGTCTG 23 0.10115670493028985 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.004398117605664775 0.0 0.0 0.0 0.0 17 0.004398117605664775 0.0 0.0 0.0 0.0 18 0.00879623521132955 0.0 0.0 0.0 0.0 19 0.00879623521132955 0.0 0.0 0.0 0.0 20 0.00879623521132955 0.0 0.0 0.0 0.0 21 0.00879623521132955 0.0 0.0 0.0 0.0 22 0.00879623521132955 0.0 0.0 0.0 0.0 23 0.013194352816994326 0.0 0.0 0.0 0.0 24 0.013194352816994326 0.0 0.0 0.0 0.0 25 0.0175924704226591 0.0 0.0 0.0 0.0 26 0.0175924704226591 0.0 0.0 0.0 0.0 27 0.0175924704226591 0.0 0.0 0.0 0.0 28 0.0175924704226591 0.0 0.0 0.0 0.0 29 0.0175924704226591 0.0 0.0 0.0 0.0 30 0.0175924704226591 0.0 0.0 0.0 0.0 31 0.0175924704226591 0.0 0.0 0.0 0.0 32 0.0175924704226591 0.0 0.0 0.0 0.0 33 0.0175924704226591 0.0 0.0 0.0 0.0 34 0.0175924704226591 0.0 0.0 0.0 0.0 35 0.0175924704226591 0.0 0.0 0.0 0.0 36 0.0175924704226591 0.0 0.0 0.0 0.0 37 0.0175924704226591 0.0 0.0 0.0 0.0 38 0.0175924704226591 0.0 0.0 0.0 0.0 39 0.0175924704226591 0.0 0.0 0.0 0.0 40 0.0175924704226591 0.0 0.0 0.0 0.0 41 0.0175924704226591 0.0 0.0 0.0 0.0 42 0.021990588028323876 0.0 0.0 0.0 0.0 43 0.021990588028323876 0.0 0.0 0.0 0.0 44 0.021990588028323876 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAACCAA 15 0.0078818165 50.694386 40 CCCCGAC 20 3.8259174E-4 50.694386 41 GCTTGCC 15 0.0078818165 50.694386 40 AAAAACC 15 0.0078818165 50.694386 38 CTCGCCT 15 0.0078818165 50.694386 41 CTTGCCT 25 1.8631874E-5 50.694378 41 CCCCCGA 25 1.8631874E-5 50.694378 40 GGACTGG 15 0.008023253 50.467564 37 GCGGTCC 15 0.008023253 50.467564 37 ACCAAGC 20 3.9119113E-4 50.467564 48 TAGCAAG 35 4.630965E-8 50.46756 47 CTAGCAA 35 4.630965E-8 50.46756 46 AGCAAGC 35 4.630965E-8 50.46756 48 CAAGCAT 25 1.9135636E-5 50.46756 49 TAGGACT 20 3.9554804E-4 50.354916 35 TCTGCGA 15 0.008094675 50.35491 45 CCTAGCA 35 4.713911E-8 50.354908 45 ATTTCAA 25 1.9391717E-5 50.354908 45 AATTTCA 15 0.008166568 50.242764 44 GTAAGAC 20 3.999434E-4 50.242764 34 >>END_MODULE