FastQCFastQC Report
Fri 17 Jun 2016
SRR1526310_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526310_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33847
Sequences flagged as poor quality0
Sequence length56
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTGAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC1060.31317398883209735No Hit
ACCTGAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCGAACCTGCGTGCAATC560.1654504091943156No Hit
ACCTGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC500.14772357963778177No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACCA203.8548693E-450.690839
ATCTATC150.00791810550.690838
TTATTCT203.8548693E-450.690838
ACCAACC150.00791810550.690838
AACTAAA203.8548693E-450.690838
TATTCTT203.8548693E-450.690839
GCCAACC203.8548693E-450.690839
AAACTAA203.9127673E-450.53834537
CATCTAT150.00801315850.53834537
TTTATTC203.9127673E-450.53834537
CCATCTA150.00815732550.3113836
CCAAAAC650.050.16119443
TTATCTT150.00825450350.16119445
TATCTTT204.06054E-450.16119446
CCTTCTC309.951091E-750.16119443
CAGCTCT150.008352539550.01190644
AGAATAT150.008352539550.01190644
TGCACTC301.0263848E-649.93759550
CCGTGGA150.00840188149.93759530
GCCTCCA150.00840188149.93759532