##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526310_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 33847 Sequences flagged as poor quality 0 Sequence length 56 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.95193074718586 39.0 37.0 39.0 33.0 40.0 2 36.32026472065471 39.0 38.0 40.0 30.0 40.0 3 36.179513693975835 39.0 38.0 40.0 29.0 40.0 4 36.10382013176943 39.0 37.0 40.0 29.0 40.0 5 35.85313321712412 39.0 36.0 39.0 28.0 40.0 6 35.548113569888024 38.0 35.0 39.0 27.0 40.0 7 35.81094336277957 38.0 36.0 39.0 29.0 40.0 8 35.75392206103938 38.0 36.0 39.0 29.0 40.0 9 35.86870328241794 38.0 36.0 39.0 29.0 40.0 10 35.18607262091175 38.0 35.0 39.0 27.0 40.0 11 33.67760805979851 37.0 31.0 39.0 23.0 40.0 12 31.915058941708274 36.0 29.0 39.0 14.0 40.0 13 31.367949892161786 36.0 28.0 39.0 12.0 40.0 14 31.185777173752474 36.0 27.0 39.0 11.0 40.0 15 31.670576417407748 36.0 28.0 39.0 14.0 40.0 16 31.938281088427335 36.0 29.0 39.0 15.0 40.0 17 32.0785889443673 36.0 29.0 39.0 16.0 40.0 18 32.04833515525748 36.0 29.0 38.0 17.0 40.0 19 33.12574231098768 36.0 31.0 39.0 23.0 40.0 20 33.05935533429846 36.0 30.0 39.0 22.0 40.0 21 34.56004963512276 38.0 34.0 39.0 25.0 40.0 22 34.300528850415105 38.0 33.0 39.0 24.0 40.0 23 34.3926197299613 38.0 33.0 39.0 25.0 40.0 24 34.00980884568795 38.0 33.0 39.0 23.0 40.0 25 34.279108931367624 38.0 33.0 39.0 25.0 40.0 26 34.832215558247405 38.0 35.0 39.0 27.0 40.0 27 34.78249180134133 38.0 35.0 39.0 27.0 40.0 28 34.69264632020563 38.0 35.0 39.0 27.0 40.0 29 34.61931633527344 38.0 35.0 39.0 26.0 40.0 30 34.562265488817324 38.0 35.0 39.0 26.0 40.0 31 34.308890005022604 38.0 34.0 39.0 25.0 40.0 32 33.15386888055071 38.0 33.0 39.0 16.0 40.0 33 34.37190888409608 38.0 35.0 39.0 25.0 40.0 34 34.10896091234083 38.0 34.0 39.0 23.0 40.0 35 34.1730434011877 38.0 34.0 39.0 25.0 40.0 36 31.782403167193547 35.0 31.0 38.0 18.0 39.0 37 31.532632138741985 35.0 31.0 38.0 15.0 39.0 38 30.904895559429196 35.0 30.0 38.0 4.0 39.0 39 28.9754483410642 33.0 27.0 38.0 4.0 39.0 40 24.370047566992643 30.0 10.0 37.0 4.0 39.0 41 20.61689366856738 23.0 8.0 31.0 4.0 36.0 42 25.195704198304135 30.0 21.0 33.0 4.0 36.0 43 20.790675687653263 24.0 7.0 32.0 4.0 36.0 44 21.780837297249388 27.0 7.0 33.0 4.0 36.0 45 24.785032646911098 30.0 18.0 33.0 4.0 37.0 46 25.031760569622122 30.0 10.0 36.0 4.0 38.0 47 24.09997931869885 30.0 5.0 35.0 4.0 38.0 48 24.166011758796937 29.0 4.0 35.0 4.0 38.0 49 21.1987768487606 25.0 4.0 35.0 4.0 38.0 50 23.958844210712915 29.0 4.0 35.0 4.0 38.0 51 25.61139835140485 32.0 4.0 38.0 4.0 40.0 52 25.42316896623039 32.0 4.0 38.0 4.0 40.0 53 25.001743138239725 32.0 4.0 38.0 4.0 40.0 54 24.073714066239255 31.0 4.0 38.0 4.0 39.0 55 24.413803291281354 31.0 4.0 38.0 4.0 40.0 56 23.555972464324757 31.0 4.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.1962275880454314 1 2 2.4144377128173957 1 3 2.6711003024811433 1 4 2.5719451409880136 1 5 2.976060220437617 1 6 3.294887849874222 1 7 2.6276439977300967 1 8 2.5381090518791822 1 9 2.6574590906134006 1 10 2.3864480542130337 1 11 -5.180627589990397 1 12 -7.676281831418347 1 13 -19.97231760036918 1 14 -17.781699440371085 1 15 -12.13965101429983 1 16 -20.802944160344484 1 17 -21.07957613875027 1 18 -19.80140709833045 1 19 -7.06965189487325 1 20 -10.74169163447976 1 21 -10.284163265124942 1 22 -4.100253154285429 1 23 -5.5172244977054845 1 24 -4.903500583110645 1 25 -5.552242146869279 1 26 2.7892774257731006 1 27 2.233045616534106 1 28 3.0291949818791437 1 29 1.8971985630345714 1 30 2.637162859397911 1 31 1.6420854350134988 1 32 -11.73579554646895 1 33 1.38716824849746 1 34 1.6893178242268547 1 35 1.9399935866596962 1 36 4.817587362573608 1 37 3.951819171792387 1 38 5.9311285150460265 1 39 6.799856674411863 1 40 8.722240686473615 1 41 -7.939793729416968 1 42 -13.072393872903774 1 43 -12.297236350446449 1 44 -15.039663835680376 1 45 -16.824763320588666 1 46 -18.72647950846603 1 47 -17.624611979052176 1 48 -17.103209020185048 1 49 -14.629123620815628 1 50 -17.032075478293905 1 51 -18.653723330296703 1 52 -18.411168002336293 1 53 -18.05565845726698 1 54 -17.758254730312828 1 55 -17.734258753050398 1 56 -16.727857196419663 2 1 1.8241345647896168 2 2 1.9834299608794126 2 3 2.9279478477007785 2 4 2.918715166827809 2 5 3.1419516933058276 2 6 3.7853271263600092 2 7 3.0312615687895317 2 8 3.375318354204765 2 9 3.0822652921637896 2 10 2.7171974082181976 2 11 -2.635407951747503 2 12 -10.096953666043671 2 13 -12.232782716648249 2 14 -15.459218820216048 2 15 -5.080736285617657 2 16 -3.9481637985873768 2 17 -2.8175606348742974 2 18 -10.743525961379547 2 19 -2.6138379413848796 2 20 -3.49414641484152 2 21 2.839867742627 2 22 2.7209354762055256 2 23 1.9163672335606634 2 24 2.035000708879018 2 25 2.8663625042935124 2 26 2.7138252293906717 2 27 2.4774900609785533 2 28 2.3284197880806943 2 29 2.8258807335772076 2 30 1.7177830144366695 2 31 -1.1708344616273259 2 32 -7.412798130448277 2 33 -0.29241831481002833 2 34 0.19164340562220872 2 35 -0.1793862583015411 2 36 4.621204933633042 2 37 4.564739068433212 2 38 4.76316985871528 2 39 7.516135744179309 2 40 10.912938360892216 2 41 5.737725650427993 2 42 1.7617146542280082 2 43 -5.676047719955493 2 44 -10.791601820176501 2 45 -1.8216625453948687 2 46 -12.130613875391097 2 47 -10.38533549326406 2 48 -9.48667155248479 2 49 -13.054446618231648 2 50 -9.035693049353336 2 51 -10.258374493087402 2 52 -9.838041387349215 2 53 -10.6825318422799 2 54 -15.492621655377427 2 55 -10.821597254342388 2 56 -8.954730581432583 3 1 1.9388886631502729 3 2 3.077419031917664 3 3 3.212465151890214 3 4 2.857877279760416 3 5 3.2086183599724976 3 6 3.7150174724437974 3 7 3.323793444927965 3 8 3.5556462230572237 3 9 3.674615018939747 3 10 3.1561773717883312 3 11 1.8491093524419284 3 12 2.8586018895118848 3 13 -3.357372880582677 3 14 -3.5422789295056667 3 15 -0.715708963213288 3 16 3.118138569354336 3 17 2.7911825345246086 3 18 2.9271479912616165 3 19 1.6451784520577455 3 20 1.5739774467249639 3 21 4.309813097818257 3 22 3.7125566055315744 3 23 3.62383535742223 3 24 3.7537620932141706 3 25 3.784395291178761 3 26 2.2227505481520566 3 27 1.872389878829189 3 28 2.442080990266483 3 29 3.2269736297520737 3 30 2.516690118261806 3 31 1.8677812032178451 3 32 1.4086954943240322 3 33 3.3341754921116475 3 34 3.106397503982862 3 35 2.344111009458018 3 36 5.333408940919018 3 37 5.545795534735582 3 38 5.520911206620561 3 39 7.98170951467111 3 40 12.119495737941396 3 41 11.033900513815972 3 42 8.170457823626915 3 43 2.2860652126492447 3 44 3.426977414796177 3 45 5.818774613075078 3 46 -2.7211421085422813 3 47 -5.822858261934371 3 48 -2.46882091496202 3 49 -9.944792702020356 3 50 -2.580683941885212 3 51 -3.8321449848906823 3 52 -3.1141798208646954 3 53 -4.135719456123251 3 54 -9.552002347545002 3 55 -6.101014376382462 3 56 -3.4636559001939666 4 1 2.056277421932471 4 2 2.948906151355601 4 3 3.4323129270658583 4 4 3.08855643666908 4 5 3.2264755028296435 4 6 3.669057285090169 4 7 3.584039346567309 4 8 3.8217469256333345 4 9 3.6084557683542684 4 10 3.149737090757881 4 11 4.497528556189003 4 12 3.5371733180833154 4 13 5.702931569066035 4 14 2.920543084545855 4 15 5.025216095334724 4 16 6.10818540776183 4 17 5.958629841316181 4 18 6.1794899116363275 4 19 4.329019201472267 4 20 4.792361521666418 4 21 4.256534409293664 4 22 4.01934817461823 4 23 4.202875170068602 4 24 4.087778486656795 4 25 4.062791075722082 4 26 2.7166030071684517 4 27 3.643759902248391 4 28 2.2260388356997396 4 29 2.1318331145295915 4 30 2.6296877763414344 4 31 3.869244903452042 4 32 3.424503456853312 4 33 3.132184859793142 4 34 3.72378626276506 4 35 2.3432327893175042 4 36 5.059696997125105 4 37 5.260373989068135 4 38 5.580630176175596 4 39 8.219707172750738 4 40 10.566509789463648 4 41 3.625820888523233 4 42 7.462905130418484 4 43 8.16165069274292 4 44 11.988160084585406 4 45 7.477146978414655 4 46 7.680497235720015 4 47 8.723394665466099 4 48 6.804598288785051 4 49 5.979680365895334 4 50 7.350814887154602 4 51 5.545196935484025 4 52 5.519895120587293 4 53 5.589690379942322 4 54 -0.42436768712345696 4 55 5.532767825022692 4 56 4.578205926503923 5 1 2.5356730263280767 5 2 3.466763294212747 5 3 3.4694008391537707 5 4 3.201193799306445 5 5 3.5932337445878844 5 6 3.88746387849676 5 7 2.8436547311826956 5 8 3.221472200358612 5 9 3.446367856266356 5 10 3.732154673175465 5 11 5.067583501243952 5 12 7.897063427973425 5 13 7.70567882181329 5 14 7.3587298977326725 5 15 7.397468842587468 5 16 6.948844748421166 5 17 7.315772698459035 5 18 6.19322617537259 5 19 5.532315904768968 5 20 6.078075807380703 5 21 4.256534409293664 5 22 4.222644877914931 5 23 3.834743301936733 5 24 4.308932332810642 5 25 4.039439427370432 5 26 2.745449161014605 5 27 3.483045616534106 5 28 3.357906967567871 5 29 3.177162784859263 5 30 2.854962501616157 5 31 3.870618529825663 5 32 4.97395400630386 5 33 3.6349321125403975 5 34 3.991643405622206 5 35 4.157793228877942 5 36 5.341290403718514 5 37 5.06943992313407 5 38 6.094366439911859 5 39 8.108443436487 5 40 6.147553745507601 5 41 1.7659307786331198 5 42 8.329663372176725 5 43 13.429507835600063 5 44 11.926346897772216 5 45 9.937311813579491 5 46 10.795881851104628 5 47 11.759108951180384 5 48 11.576576310763073 5 49 12.108801245016213 5 50 11.29037532671504 5 51 9.914702429989518 5 52 9.388026988719162 5 53 9.878151918403862 5 54 9.603104840349069 5 55 9.550624967879834 5 56 8.321337794635792 6 1 2.3751549729528847 6 2 3.247375539110706 6 3 3.4807823148209636 6 4 3.262025824424178 6 5 3.4739244824214737 6 6 3.5032409585595587 6 7 3.290671999628536 6 8 3.2732775378782364 6 9 3.4809047479460986 6 10 3.685451376472166 6 11 5.505181617413491 6 12 7.65452025685882 6 13 7.146809120086441 6 14 7.698604309035652 6 15 7.777767115742886 6 16 7.062267040414888 6 17 6.56067065764271 6 18 6.541734809595507 6 19 5.512692670860016 6 20 6.185218664523557 6 21 4.506534409293664 6 22 4.896899195026393 6 23 3.6365486394563575 6 24 4.600533588697608 6 25 4.499015565518 6 26 3.581398925535801 6 27 3.784066024697374 6 28 3.6086918969242276 6 29 3.0068331145295915 6 30 3.573565327361841 6 31 4.279959189166327 6 32 5.480625905832898 6 33 3.9434093495890608 6 34 4.297765854601799 6 35 4.1825185036032195 6 36 4.460207201206739 6 37 4.130271948251803 6 38 5.325528135359271 6 39 7.528380642138494 6 40 2.472122034361604 6 41 -0.7058117645379944 6 42 6.490966354908281 6 43 12.169303753967409 6 44 10.868262125401731 6 45 7.428677590659554 6 46 9.649884990822056 6 47 10.993802828731408 6 48 11.18725135000954 6 49 12.403149753650439 6 50 10.141631213685212 6 51 11.313054078341167 6 52 10.616833896097496 6 53 11.604996502391298 6 54 11.912367006754092 6 55 10.310829049512485 6 56 11.340961028544744 7 1 2.4972114878665366 7 2 3.6975325249819804 7 3 3.9694008391537707 7 4 3.451193799306445 7 5 3.477849129203264 7 6 4.002848493881373 7 7 3.3436547311826956 7 8 3.8176260465124585 7 9 3.984829394727889 7 10 3.828308519329312 7 11 5.5483527320131785 7 12 7.377832658742651 7 13 8.12875574489021 7 14 7.8587298977326725 7 15 7.705161150279775 7 16 6.6026909022673195 7 17 6.527311159997495 7 18 6.885533867680284 7 19 5.301546673999734 7 20 6.039614268919163 7 21 3.7757651785244306 7 22 4.741875647145697 7 23 3.623204840398273 7 24 4.9435477174260285 7 25 4.520208658139666 7 26 3.033910699476145 7 27 3.1368917703802595 7 28 4.107906967567871 7 29 3.8502397079361828 7 30 3.9318855785392373 7 31 4.120618529825663 7 32 5.031646313996166 7 33 4.327239804848091 7 34 4.895489559468359 7 35 3.8885624596471757 7 36 3.89898271141082 7 37 3.2425168462109895 7 38 3.8251356706810853 7 39 5.608443436487001 7 40 -1.4101385621847058 7 41 -7.310992298289955 7 42 6.329663372176725 7 43 11.73720014329237 7 44 11.022500743926063 7 45 6.687311813579491 7 46 10.276651081873862 7 47 10.662955105026537 7 48 10.92273015691692 7 49 12.43572432193929 7 50 9.386529172868887 7 51 11.568548583835678 7 52 10.772642373334548 7 53 10.878151918403862 7 54 11.756950994195225 7 55 11.916009583264447 7 56 11.302107025405025 8 1 2.5692326040053004 8 2 3.383429960879411 8 3 3.773699481687707 8 4 3.7370158204225774 8 5 3.9733242423254396 8 6 4.692516668843666 8 7 3.856098170096722 8 8 4.081200707145939 8 9 3.5685398019677095 8 10 3.9263477350155895 8 11 5.782892701847267 8 12 7.27036659539424 8 13 8.186825126489008 8 14 8.493722356254544 8 15 7.813381361441163 8 16 6.468829665464909 8 17 7.0804785808119775 8 18 6.540134169339407 8 19 5.4410640193994375 8 20 5.0731738465963865 8 21 3.97712264458778 8 22 4.092177306270891 8 23 3.4640796518613186 8 24 3.8206216239116984 8 25 3.729107602332725 8 26 3.3072892816782584 8 27 2.350692675357635 8 28 2.9088119449434373 8 29 2.057253282596818 8 30 2.6079790928680424 8 31 3.537662270398819 8 32 4.855175725760869 8 33 2.694509789765135 8 34 3.6779179154261286 8 35 1.871594133855318 8 36 1.1636885937637622 8 37 0.8601639050345185 8 38 1.6716665907414203 8 39 3.5043710382969557 8 40 -2.879218501852879 8 41 -9.332862584866124 8 42 5.3224989679535 8 43 10.777547051286337 8 44 9.169182493548991 8 45 6.225396278134543 8 46 9.180497235720015 8 47 10.572834441376461 8 48 10.680648708953122 8 49 13.399148153010184 8 50 10.798293878751242 8 51 11.46711570299103 8 52 11.905749462323989 8 53 12.124004105432515 8 54 12.394960043968982 8 55 12.27121320317395 8 56 11.824354385887677 9 1 2.020212996162165 9 2 2.2853907451931406 9 3 3.656052422864178 9 4 3.3448589576774808 9 5 2.8948928697764202 9 6 3.4768303943338665 9 7 3.169823660292799 9 8 2.5321810993028038 9 9 3.117559409810845 9 10 2.0439947938391185 9 11 4.704461329298248 9 12 6.701739144413846 9 13 6.951531008841943 9 14 8.17999686605846 9 15 7.323185283009796 9 16 6.057064959582554 9 17 6.100086423949229 9 18 6.049938090908032 9 19 3.009691470379824 9 20 4.230036591694425 9 21 3.447710879881896 9 22 4.6019812278395165 9 23 2.7974129851946543 9 24 3.271602016068563 9 25 2.96440171997979 9 26 2.3661128110900194 9 27 2.115398557710577 9 28 0.8892041018061789 9 29 1.1945081845575984 9 30 1.411900661495494 9 31 0.537662270398819 9 32 1.9139992551726372 9 33 1.1847058681965024 9 34 0.7367414448378895 9 35 0.08728040836512463 9 36 -0.1696447395695735 9 37 1.017026650132557 9 38 1.1226469828982815 9 39 2.4259396657479364 9 40 -0.2125518351862148 9 41 -1.5289410162386723 9 42 6.675440144424087 9 43 11.012841168933395 9 44 8.424084454333304 9 45 6.166572748722778 9 46 7.317752137680799 9 47 7.788520715886268 9 48 7.700256552090373 9 49 10.654050113794494 9 50 7.327705643457122 9 51 7.133782369657695 9 52 7.9645729917357535 9 53 6.673023713275654 9 54 8.120450240047411 9 55 8.094742614938657 9 56 8.353766150593561 10 1 1.9730707350023806 10 2 2.8869760601701913 10 3 3.1842126231144903 10 4 3.1721430628088996 10 5 3.336277934440581 10 6 3.4893460388895576 10 7 3.4643584955034825 10 8 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114 55 -2.8200646872925788 114 56 1.9433537097551543 115 1 1.6389493796044334 115 2 2.679726257175709 115 3 2.913133032885966 115 4 2.7261225742352195 115 5 3.0975072488613833 115 6 3.570512311545194 115 7 2.764594902122866 115 8 2.745688724575139 115 9 2.8452282551267487 115 10 2.0505307415515333 115 11 0.1423698260302757 115 12 -3.585842554932558 115 13 -0.42537530924084166 115 14 -1.4888484498456762 115 15 0.6674118625304892 115 16 -2.7926082430318218 115 17 -3.573116190429854 115 18 2.612029594176011 115 19 1.0750509475040069 115 20 0.7354832147881076 115 21 2.6176455204047784 115 22 2.728342883612939 115 23 2.8867376039310315 115 24 3.768334042212352 115 25 2.607103245034253 115 26 2.832343747909192 115 27 3.196008579497068 115 28 2.4839753436362457 115 29 3.4777325854290595 115 30 3.08074597739963 115 31 3.3328692420763772 115 32 -5.486872204522353 115 33 2.7075816851899717 115 34 2.584235998214801 115 35 2.865058186142903 115 36 3.6582419706700797 115 37 4.22399832769247 115 38 5.207614303159723 115 39 7.990209818253383 115 40 9.624049472003332 115 41 7.952540465242809 115 42 3.3246776171909715 115 43 0.5313596874519142 115 44 1.4306204020457223 115 45 5.57833745460513 115 46 -1.5972805420577636 115 47 -7.555705863634431 115 48 -3.6940789598921953 115 49 -6.1062984700835 115 50 1.275418061757776 115 51 2.2971810624681552 115 52 3.3915882422804167 115 53 1.2656163058682495 115 54 0.5518227890670175 115 55 0.763587930842796 115 56 4.704528677826676 116 1 1.372521661563809 116 2 2.8780536167933946 116 3 2.627589424761716 116 4 3.0355610449640125 116 5 3.0150699728757218 116 6 3.3459006030625176 116 7 2.563878056244725 116 8 1.8914473864628292 116 9 2.634953464206802 116 10 2.0218569064260876 116 11 -0.6712502456543135 116 12 -0.30268843306875937 116 13 -2.929556910196638 116 14 -9.848466132044006 116 15 -2.1366502641122835 116 16 -12.376837633712825 116 17 -11.619711172508705 116 18 -4.821662162096395 116 19 -3.975128263965523 116 20 -4.446117741006393 116 21 -0.783788171351496 116 22 0.0377813543417318 116 23 0.9643959073957902 116 24 0.7611655834309872 116 25 0.7373302462289928 116 26 1.41418365232974 116 27 0.9749811004050741 116 28 1.16497892786564 116 29 2.260289335727748 116 30 2.289826025189356 116 31 0.754613567046512 116 32 -10.774805298907058 116 33 -0.4357874904372707 116 34 0.5722885669125262 116 35 -0.8266980862585314 116 36 2.7538214210882366 116 37 2.903807168791637 116 38 3.8778651992170694 116 39 6.206458324824467 116 40 9.545196425408346 116 41 9.44955360741724 116 42 2.5660157294968258 116 43 -8.836621196658001 116 44 -2.071171712649619 116 45 2.3095202503040575 116 46 -2.271115667505793 116 47 -6.6118587907551 116 48 -5.0214385775743935 116 49 -6.634374933643835 116 50 -0.9037934077762735 116 51 0.9996900230416301 116 52 -0.24162561673989558 116 53 -2.434503168444774 116 54 -4.3621557055566385 116 55 -1.328407290184682 116 56 0.8610400278864141 117 1 2.1770757412602038 117 2 3.069704470683334 117 3 3.303111246393591 117 4 3.1095648400304228 117 5 3.3850889482077946 117 6 3.0650656884515115 117 7 2.856098170096722 117 8 3.022377177734178 117 9 3.2744221549088905 117 10 3.416543813446957 117 11 -7.472009258937046 117 12 -14.141398110488115 117 13 -3.597488599001192 117 14 -5.349414898647421 117 15 -7.814069618950992 117 16 -8.923327197280187 117 17 -4.9195214191880225 117 18 0.5597420124766614 117 19 -0.9314850002084079 117 20 -1.9072183102663551 117 21 1.330063821058367 117 22 1.0529616199963812 117 23 1.0327071028417123 117 24 -1.904868572166734 117 25 1.7487154454699834 117 26 3.0916030071684517 117 27 3.93892796947528 117 28 3.8499884155316693 117 29 3.763135635537992 117 30 4.392292818358236 117 31 3.5572701135360703 117 32 -7.79188309776854 117 33 2.4199999858435604 117 34 2.050466935033974 117 35 2.577476486796492 117 36 5.634276829057878 117 37 5.683693316799221 117 38 6.2991175711335785 117 39 8.73966515594401 117 40 9.218820713833392 117 41 7.098509964153482 117 42 4.224459752267226 117 43 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118 26 1.684195599761047 118 27 2.047860431348923 118 28 0.7061975658584672 118 29 0.4406955483920214 118 30 2.1918570885107442 118 31 1.740276649483782 118 32 -6.190575908226055 118 33 2.485359462967743 118 34 1.9175693315481297 118 35 1.1243174454021627 118 36 4.732316044744156 118 37 5.7425168462109895 118 38 5.911318006863425 118 39 8.582802410845972 118 40 10.438864286818141 118 41 8.91550342820577 118 42 3.917270209783563 118 43 2.0869152430074713 118 44 -2.1990092275839075 118 45 3.615374491642168 118 46 -7.152836097613319 118 47 -11.555705863634431 118 48 -8.3977826635959 118 49 -7.513705877490908 118 50 -5.391248604908892 118 51 -4.29541153012444 118 52 -4.349152498460324 118 53 -8.067717027465084 118 54 -10.040769803525574 118 55 -8.717893550638685 118 56 -5.517693544395547 119 1 0.9604982011532499 119 2 1.85312693057638 119 3 2.0410791608320977 119 4 1.4197252678379115 119 5 1.8904365417906774 119 6 2.602498843531727 119 7 1.5726757102036757 119 8 1.7844092632956716 119 9 0.8519622618607556 119 10 1.3737630647838586 119 11 -7.185913002252555 119 12 -9.414125383215389 119 13 -2.4691463530118867 119 14 -0.48043094142816756 119 15 -7.677705982587355 119 16 -7.115840566264147 119 17 -6.911500028813691 119 18 -1.8172633351169196 119 19 -1.6774743050212422 119 20 -1.262833283528387 119 21 -0.26619286343360926 119 22 -0.48714533187528275 119 23 -0.215955998762567 119 24 -3.696312422434115 119 25 -0.8154556775246675 119 26 1.7734211889866316 119 27 1.5512274347159263 119 28 2.5344803941412977 119 29 2.3834564911529696 119 30 2.0420254386790972 119 31 0.7604786696858028 119 32 -13.10471732236747 119 33 0.8691978468061308 119 34 -0.2810838671050675 119 35 1.2994016204863357 119 36 4.580800893229 119 37 3.787971391665536 119 38 5.061149656695072 119 39 7.8706811987247605 119 40 10.831120179074038 119 41 7.668028680731023 119 42 1.3314116239249785 119 43 -2.6841285280363003 119 44 -5.458268486843167 119 45 -3.391359515091839 119 46 -8.546775491552712 119 47 -9.468163776092343 119 48 -10.412934178747415 119 49 -10.92616378994882 119 50 -11.431652645312932 119 51 -12.07655631126922 119 52 -13.152182801490628 119 53 -13.569400529148586 119 54 -14.190601453357225 119 55 -14.65392048666562 119 56 -13.923417450119452 120 1 1.2154389126157028 120 2 2.108067642038833 120 3 2.558865722096918 120 4 2.4829663745572788 120 5 2.686879229537716 120 6 3.134119396891407 120 7 2.2959958683064414 120 8 2.810100962900414 120 9 2.0416855820188644 120 10 0.9152650410684444 120 11 -9.326229207786152 120 12 -2.9674850670098536 120 13 -9.137130542735207 120 14 -9.960668095578367 120 15 -10.944504401559687 120 16 -9.439951238200905 120 17 -10.771183823280094 120 18 -4.325168473456841 120 19 -3.7703596805153126 120 20 -4.584967670880168 120 21 -0.9717264602715545 120 22 -0.5148133160650019 120 23 -0.7396714137823324 120 24 -5.004612817691029 120 25 -1.3945070609238783 120 26 1.2655160506467098 120 27 0.6678282252297549 120 28 0.7400139909792429 120 29 0.9881995741569227 120 30 0.6250293912482618 120 31 -1.496438326361627 120 32 -12.055310207742963 120 33 -0.707877252008096 120 34 -9.443139203073446 120 35 -0.854748577142125 120 36 -2.3836259842413554 120 37 -3.127048371180315 120 38 -1.4348977741349636 120 39 1.3943966137445258 120 40 -0.48706163910778244 120 41 2.891348838833789 120 42 -1.261473751569092 120 43 -0.14657912092167535 120 44 -5.893051095538819 120 45 -7.798474139597767 120 46 -11.167328851236507 120 47 -12.566978005341355 120 48 -8.94850730127706 120 49 -13.785847584415224 120 50 -8.396079522783287 120 51 -8.040983188739576 120 52 -7.059297425996556 120 53 -9.138570489622895 120 54 -12.82498880513588 120 55 -10.460244597337557 120 56 -7.549899663558188 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 925.0 5 65.0 6 83.0 7 40.0 8 53.0 9 50.0 10 37.0 11 40.0 12 59.0 13 61.0 14 108.0 15 144.0 16 172.0 17 215.0 18 270.0 19 351.0 20 461.0 21 530.0 22 680.0 23 810.0 24 877.0 25 905.0 26 1058.0 27 1075.0 28 1230.0 29 1241.0 30 1391.0 31 1592.0 32 1884.0 33 2255.0 34 2766.0 35 3231.0 36 3723.0 37 3431.0 38 1589.0 39 445.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 100.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 99.5863739770142 0.36340000590894317 0.0413626022985789 0.008863414778266908 8 99.45046828374745 0.44021626732058977 0.062043903447868344 0.04727154548409017 9 97.7481604066314 1.2943644906764384 0.48760306155619254 0.46987204113596737 10 40.623984400389986 37.19088840960794 9.294767630809229 12.890359559192838 11 26.782284988329835 31.683753360711435 20.04608975684699 21.487871894111738 12 35.48615830058794 15.481431146039531 20.592667001506783 28.43974355186575 13 26.548881732502146 14.890536827488404 24.991875203119925 33.56870623688953 14 21.569786341203937 18.638257631726706 26.57584443984751 33.216111587221846 15 23.776824034334766 20.355187213260322 25.221252034926746 30.646736717478174 16 24.691102571681938 19.266922849541825 26.757315991723324 29.284658587052913 17 26.592601347358467 25.378205885829097 20.561990308474176 27.467202458338257 18 27.195366977898594 27.7892684079896 20.895875192057677 24.11948942205413 19 25.501551189245085 28.713251588122326 17.840153641601418 27.94504358103117 20 26.177035614009164 26.245012560957587 19.875868183833308 27.70208364119994 21 25.340543096061225 23.762668794137635 23.641521141742754 27.25526696805839 22 27.175229710166338 24.096670310514963 22.61648004254439 26.111619936774304 23 28.194522409667034 25.009602032676455 24.761426418884984 22.03444913877153 24 22.702159718734304 26.138210181109105 24.09962478210772 27.06000531804887 25 24.679430361047096 26.401938190628137 24.729657862081194 24.188973586243574 26 23.852369254446977 25.607482167697636 28.564240684286858 21.975907893568532 27 21.7319343874686 20.425594798285797 31.328505984926853 26.513964829318752 28 20.468199709964782 22.0811506703365 34.72135902216698 22.72929059753174 29 19.482208298853294 21.93817236079915 35.426764392954254 23.15285494739331 30 18.242005083643672 22.843884849559615 40.08393923272448 18.830170834072234 31 17.65716146990021 23.44319090343786 41.04409108406621 17.855556542595718 32 21.285650953155017 17.756760750701936 40.97236589330575 19.985222402837298 33 19.64010891440748 19.977506807150466 43.548005208949924 16.83437906949213 34 18.277248802412917 19.20279141285706 47.4037494825241 15.116210302205927 35 18.757943897608705 21.172888770654133 46.1292897047087 13.939877627028466 36 16.701677564424983 20.260954466108462 45.79425426787775 17.243113701588804 37 16.101619636988126 20.80656145441625 46.16409557930892 16.92772332928671 38 18.48224852071006 22.662721893491124 45.60650887573964 13.248520710059172 39 17.5165376286672 18.050487971285335 40.93619293405713 23.49678146599033 40 11.002408635403967 16.07541110351185 32.50468345772994 40.41749680335425 41 13.861711013005609 16.940102613053334 35.15391958000239 34.04426679393867 42 14.15546740867918 24.12823397075366 47.12272808004263 14.593570540524539 43 9.463310377582522 14.17715507005462 36.380907147945855 39.978627404417004 44 11.30522625308257 15.476721038714086 33.238256529102415 39.97979617910093 45 15.168007572172268 21.083767155702795 47.37044486512068 16.37778040700426 46 14.552658033232808 21.885163503045355 48.208266808586124 15.353911655135711 47 14.504019862851738 21.272759517616457 43.64211397493497 20.58110664459683 48 14.665365969019748 23.119900673997872 47.03795672224193 15.176776634740452 49 11.29367103624177 16.652826383534016 32.685805801055814 39.3676967791684 50 13.830259613392142 23.092862851898996 49.471596459548266 13.605281075160594 51 17.043029871977243 23.49158368895211 44.60941204362257 14.85597439544808 52 15.232475388447398 26.23354287747598 43.5950658284901 14.938915905586525 53 15.213852301317152 26.29273346159538 44.50791771496226 13.985496522125205 54 13.552148691843257 23.271575707351722 40.42263525512016 22.753640345684857 55 15.271037992661855 24.106403124630134 47.147591430938576 13.47496745176944 56 14.952192060625796 25.07326603712146 43.58071105059057 16.393830851662177 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.0 7 2.0 8 3.5 9 5.0 10 4.5 11 4.0 12 11.5 13 39.0 14 59.0 15 83.0 16 107.0 17 170.5 18 234.0 19 268.5 20 303.0 21 364.0 22 463.0 23 501.0 24 660.0 25 819.0 26 1045.5 27 1272.0 28 1457.0 29 1786.0 30 1930.0 31 2007.5 32 2085.0 33 1946.5 34 1808.0 35 1655.0 36 1502.0 37 1316.0 38 969.0 39 808.0 40 764.5 41 721.0 42 663.0 43 605.0 44 608.5 45 612.0 46 631.0 47 697.5 48 745.0 49 798.5 50 852.0 51 850.5 52 849.0 53 903.0 54 1029.5 55 1102.0 56 1146.0 57 1190.0 58 1284.0 59 1378.0 60 1463.0 61 1548.0 62 1581.5 63 1573.0 64 1531.0 65 1513.0 66 1495.0 67 1276.5 68 1058.0 69 930.5 70 803.0 71 689.5 72 472.5 73 369.0 74 300.5 75 232.0 76 193.0 77 154.0 78 121.5 79 64.5 80 40.0 81 27.5 82 15.0 83 8.5 84 2.0 85 1.5 86 1.0 87 2.0 88 1.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.023635772742045084 10 0.0 11 0.0 12 0.0 13 0.0 14 0.023635772742045084 15 0.1831772387508494 16 0.05022601707684581 17 0.008863414778266908 18 0.008863414778266908 19 0.005908943185511271 20 0.03545365911306763 21 0.011817886371022542 22 0.0 23 0.0 24 0.0 25 0.0029544715927556356 26 0.17726829556533813 27 0.03545365911306763 28 0.17135935237982686 29 0.032499187520311994 30 0.03840813070582326 31 0.2245398410494283 32 0.03545365911306763 33 0.17431382397258252 34 0.08567967618991343 35 0.04727154548409017 36 0.14181463645227052 37 0.218630897863917 38 0.13886016485951486 39 0.4018081366147665 40 0.6440748072207286 41 0.9542943244600702 42 0.1920406535291163 43 0.4697609832481461 44 0.5613496026235708 45 0.11522439211746979 46 0.07386178981889088 47 0.04431707389133453 48 0.05613496026235708 49 0.381126835465477 50 0.19499512512187195 51 0.30431057405383044 52 0.36340000590894317 53 0.1831772387508494 54 0.17431382397258252 55 0.15067805123053743 56 0.19499512512187195 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 33847.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.1232014654179 #Duplication Level Percentage of deduplicated Percentage of total 1 94.57448203514294 85.23355097940734 2 3.730658274324679 6.724377345111828 3 0.8752950432730133 2.366531745797264 4 0.3179910831366378 1.1463349779891865 5 0.15407815368476266 0.6943008242975744 6 0.08195646472593758 0.44317073891334535 7 0.059008654602675056 0.3722634206872101 8 0.03606084447941254 0.25999350016249595 9 0.022947810123262524 0.18613171034360504 >10 0.14096511932861266 2.0947203592637456 >50 0.0032782585890375033 0.1654504091943156 >100 0.0032782585890375033 0.31317398883209735 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACCTGAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC 106 0.31317398883209735 No Hit ACCTGAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCGAACCTGCGTGCAATC 56 0.1654504091943156 No Hit ACCTGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC 50 0.14772357963778177 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.008863414778266908 0.0 0.0 0.0 0.0 11 0.017726829556533815 0.0 0.0 0.0 0.0 12 0.020681301149289448 0.0 0.0 0.0 0.0 13 0.020681301149289448 0.0 0.0 0.0 0.0 14 0.02659024433480072 0.0 0.0 0.0 0.0 15 0.032499187520311994 0.0 0.0 0.0 0.0 16 0.032499187520311994 0.0 0.0 0.0 0.0 17 0.041362602298578896 0.0 0.0 0.0 0.0 18 0.041362602298578896 0.0 0.0 0.0 0.0 19 0.04727154548409017 0.0 0.0 0.0 0.0 20 0.050226017076845805 0.0 0.0 0.0 0.0 21 0.05613496026235708 0.0 0.0 0.0 0.0 22 0.059089431855112715 0.0 0.0 0.0 0.0 23 0.06204390344786835 0.0 0.0 0.0 0.0 24 0.06499837504062399 0.0 0.0 0.0 0.0 25 0.07090731822613526 0.0 0.0 0.0 0.0 26 0.07977073300440217 0.0 0.0 0.0 0.0 27 0.07977073300440217 0.0 0.0 0.0 0.0 28 0.09454309096818034 0.0 0.0 0.0 0.0 29 0.09454309096818034 0.0 0.0 0.0 0.0 30 0.09454309096818034 0.0 0.0 0.0 0.0 31 0.09749756256093597 0.0 0.0 0.0 0.0 32 0.09749756256093597 0.0 0.0 0.0 0.0 33 0.09749756256093597 0.0 0.0 0.0 0.0 34 0.09749756256093597 0.0 0.0 0.0 0.0 35 0.09749756256093597 0.0 0.0 0.0 0.0 36 0.09749756256093597 0.0 0.0 0.0 0.0 37 0.09749756256093597 0.0 0.0 0.0 0.0 38 0.09749756256093597 0.0 0.0 0.0 0.0 39 0.09749756256093597 0.0 0.0 0.0 0.0 40 0.09749756256093597 0.0 0.0 0.0 0.0 41 0.10045203415369161 0.0 0.0 0.0 0.0 42 0.10045203415369161 0.0 0.0 0.0 0.0 43 0.10045203415369161 0.0 0.0 0.0 0.0 44 0.10636097733920288 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAACCA 20 3.8548693E-4 50.6908 39 ATCTATC 15 0.007918105 50.6908 38 TTATTCT 20 3.8548693E-4 50.6908 38 ACCAACC 15 0.007918105 50.6908 38 AACTAAA 20 3.8548693E-4 50.6908 38 TATTCTT 20 3.8548693E-4 50.6908 39 GCCAACC 20 3.8548693E-4 50.6908 39 AAACTAA 20 3.9127673E-4 50.538345 37 CATCTAT 15 0.008013158 50.538345 37 TTTATTC 20 3.9127673E-4 50.538345 37 CCATCTA 15 0.008157325 50.31138 36 CCAAAAC 65 0.0 50.161194 43 TTATCTT 15 0.008254503 50.161194 45 TATCTTT 20 4.06054E-4 50.161194 46 CCTTCTC 30 9.951091E-7 50.161194 43 CAGCTCT 15 0.0083525395 50.011906 44 AGAATAT 15 0.0083525395 50.011906 44 TGCACTC 30 1.0263848E-6 49.937595 50 CCGTGGA 15 0.008401881 49.937595 30 GCCTCCA 15 0.008401881 49.937595 32 >>END_MODULE