FastQCFastQC Report
Fri 17 Jun 2016
SRR1526309_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526309_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32007
Sequences flagged as poor quality0
Sequence length56
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGGAGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG1670.5217608648108226No Hit
TGCGGAGGGATGAGATGGACTTAAATGAATTGACGGGTACCCACAGTTCTGAGTCG1620.5061392820320555No Hit
TGCGGAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG1400.43740431780548No Hit
TGCGGAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG1370.4280313681382198No Hit
TGCGGAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG1330.41553410191520607No Hit
TGCGGAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG1190.371793670134658No Hit
TGCGGAGGGATGAGATGGACTCAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG970.3030587059080826No Hit
TGCGGAGGGATGAGATGGACTCAAATGAATTGACGGGTACCCACAGTTCTGAGTCG940.2936857562408223No Hit
TGCGGAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG760.2374480582372606No Hit
TGCGGAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG740.23119942512575373No Hit
TGCGGAGGGATGAGATGGACTCAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG650.2030805761239729No Hit
TGCGGAGGGATGCGCTGGACTTAAAGGAATTGACGGGTCCGCCCAGTTCTGAGTCG610.19058330990095915No Hit
TGCGGAGGGATGCGCTGGACTTAAATGAATTGACGGGTCCGCCCAGTTCTGAGTCG580.18121036023369888No Hit
TGCGGAGGGATGAGATGGACTCAAATGAATTGACGGGTACGCACAGTTCTGAGTCG560.17496172712219202No Hit
TGCGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA440.13746992845315087No Hit
TGCGGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA410.12809697878589058No Hit
TGCGGAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCCTGCGCTT340.10622676289561658No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTAT150.00794581350.63955338
TCCGGCC150.00794581350.63955338
GCCCTTG150.00794581350.63955338
TCCGCCC700.050.6395538
CACTGAC150.00799614550.55891841
TCAAGAC150.00804671450.4785434
GTCAACA150.00804671450.4785436
CGGTCAA150.00804671450.4785434
CGATCCT203.9324068E-450.4785436
TGCAATC204.0261226E-450.23892650
ACGGGTC1000.050.23892633
ACTGACT150.00819984650.23892242
TTCGATG150.00819984650.23892250
GCCAGTT150.00819984650.23892242
GCTGAGT204.0577524E-450.1595648
GTGCAAT150.00825136950.15955749
ACTTTCG150.00825136950.15955747
CGTGCGC150.00825136950.15955748
CGTGCAA150.00825136950.15955748
GCTCTGA252.0032676E-550.15955746