##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526309_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 32007 Sequences flagged as poor quality 0 Sequence length 56 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.37454306870372 39.0 38.0 39.0 33.0 40.0 2 36.62423844783954 39.0 36.0 39.0 31.0 40.0 3 36.874621176617616 39.0 37.0 39.0 32.0 40.0 4 36.52897803605461 39.0 36.0 39.0 31.0 40.0 5 36.54269378573437 39.0 36.0 39.0 31.0 40.0 6 34.16499515730934 38.0 35.0 39.0 20.0 40.0 7 36.270253382072674 38.0 35.0 39.0 31.0 40.0 8 36.07364014121911 38.0 35.0 39.0 30.0 40.0 9 34.99884400287437 38.0 35.0 39.0 26.0 40.0 10 34.975474115037336 38.0 35.0 39.0 27.0 40.0 11 33.750648295685316 36.0 31.0 39.0 24.0 40.0 12 32.355391008216955 36.0 29.0 39.0 18.0 40.0 13 31.850282750648297 36.0 29.0 38.0 16.0 40.0 14 31.3821351579342 36.0 27.0 38.0 14.0 40.0 15 32.047677070640795 36.0 29.0 38.0 17.0 40.0 16 32.48142593807604 36.0 30.0 38.0 18.0 40.0 17 32.61770862623801 36.0 30.0 38.0 18.0 40.0 18 32.60380541756491 36.0 30.0 38.0 19.0 40.0 19 33.31764926422345 36.0 31.0 38.0 23.0 40.0 20 33.65545037023151 36.0 31.0 38.0 24.0 40.0 21 35.02433842596932 38.0 35.0 39.0 27.0 40.0 22 34.64913925078889 38.0 34.0 39.0 25.0 40.0 23 34.737588652482266 38.0 34.0 39.0 26.0 40.0 24 34.32583497359953 38.0 33.0 39.0 23.0 40.0 25 34.6611991126941 38.0 33.0 39.0 26.0 40.0 26 34.796669478551564 38.0 35.0 39.0 26.0 40.0 27 34.847783297403694 38.0 35.0 39.0 27.0 40.0 28 34.55950260880432 38.0 34.0 39.0 26.0 40.0 29 34.483175555347266 38.0 34.0 39.0 25.0 40.0 30 34.23304277189365 38.0 33.0 39.0 24.0 40.0 31 34.222919986253004 38.0 33.0 39.0 24.0 40.0 32 33.0707032836567 37.0 32.0 39.0 18.0 40.0 33 34.33870715780923 38.0 34.0 39.0 25.0 40.0 34 34.30027806417346 38.0 34.0 39.0 25.0 40.0 35 34.36313931327522 38.0 34.0 39.0 26.0 40.0 36 32.39638204142844 35.0 31.0 38.0 21.0 39.0 37 32.17258724653982 35.0 31.0 38.0 19.0 39.0 38 30.87633955072328 35.0 30.0 38.0 12.0 39.0 39 29.352922798137907 34.0 27.0 38.0 6.0 39.0 40 25.687255912769082 32.0 12.0 37.0 4.0 39.0 41 21.960289936576373 26.0 9.0 33.0 4.0 37.0 42 25.460024369669135 30.0 19.0 34.0 4.0 37.0 43 22.373324585246976 27.0 9.0 33.0 4.0 37.0 44 23.32477270597057 28.0 10.0 33.0 4.0 37.0 45 25.482113287718313 30.0 20.0 34.0 4.0 37.0 46 25.729684131596212 31.0 17.0 36.0 4.0 38.0 47 24.775892773455805 30.0 10.0 36.0 4.0 38.0 48 24.84556503264911 30.0 11.0 36.0 4.0 38.0 49 22.521760864810823 28.0 5.0 35.0 4.0 38.0 50 24.779579466991596 30.0 10.0 35.0 4.0 38.0 51 26.056987533976944 32.0 4.0 38.0 4.0 39.0 52 25.850688911800543 32.0 4.0 38.0 4.0 39.0 53 25.582591308151343 32.0 4.0 38.0 4.0 39.0 54 24.610835129815353 31.0 4.0 37.0 4.0 39.0 55 24.769800356172087 31.0 4.0 37.0 4.0 39.0 56 24.518167900771708 32.0 4.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.1329459643613404 1 2 2.1113332206829085 1 3 1.8717078624388463 1 4 2.1711811211240075 1 5 2.2315510738564726 1 6 5.309814672126194 1 7 2.9025501298116723 1 8 2.9913417466808667 1 9 3.0099365408545964 1 10 2.490711969566455 1 11 -4.020154623486562 1 12 -6.537233188451086 1 13 -21.43764459578842 1 14 -17.535487959737694 1 15 -12.132101793421334 1 16 -21.256220718402588 1 17 -20.4878217929817 1 18 -18.787117339036854 1 19 -5.960520867462446 1 20 -10.584417592111087 1 21 -6.4757401326016115 1 22 -1.2347814591948776 1 23 -1.445630863442446 1 24 -1.473145311942254 1 25 -1.1455271107644052 1 26 3.179008766880017 1 27 3.200419682917463 1 28 3.172516811188082 1 29 3.236416549585705 1 30 2.613110590105464 1 31 2.399019022526602 1 32 -11.472343632178301 1 33 1.663309427490347 1 34 1.1121014897252621 1 35 2.0869008119600707 1 36 3.4820362211415627 1 37 4.2373726121149 1 38 5.542068782712374 1 39 6.421400495291724 1 40 7.891884721440331 1 41 -0.5531736157430807 1 42 -5.62681447522775 1 43 -12.472747597439824 1 44 -16.149940556822084 1 45 -12.43124514527988 1 46 -17.50776826861841 1 47 -17.286237947839176 1 48 -15.310079064418531 1 49 -13.169916358178476 1 50 -15.053606507780435 1 51 -15.40292686221011 1 52 -14.763706640455101 1 53 -15.383508050418133 1 54 -17.970175474601948 1 55 -16.143005557820207 1 56 -15.676026305284248 2 1 1.341502114093963 2 2 2.069889370415531 2 3 1.95726935976505 2 4 1.9358870034769495 2 5 2.2128344963163684 2 6 5.2443066507358225 2 7 2.708699862431992 2 8 3.0969567199429022 2 9 3.4618081986086082 2 10 2.8463269428284903 2 11 -3.5201546234865617 2 12 -10.466377573477825 2 13 -15.009837109157404 2 14 -17.0114237886147 2 15 -5.253759547432029 2 16 -2.1987340873865477 2 17 -1.1161640389710037 2 18 -9.434176162566267 2 19 -1.768007498478486 2 20 -3.179337378207343 2 21 2.7916395465427755 2 22 2.037945813532396 2 23 1.839128494846321 2 24 2.758138110517642 2 25 2.956077167310461 2 26 2.173661173297134 2 27 2.1776924101901898 2 28 1.4305382015624133 2 29 1.8914967634894495 2 30 3.4192603227257834 2 31 -0.5167563785429152 2 32 -10.829295503836054 2 33 -0.5546050110123275 2 34 -0.33174877765441835 2 35 -0.41978368001853994 2 36 4.470004135580069 2 37 5.063575820671051 2 38 5.25196183084071 2 39 6.393325628981568 2 40 9.123168143900227 2 41 8.093885207786332 2 42 2.7889609258417707 2 43 -1.8952074904879517 2 44 -10.775609006019945 2 45 -1.5916729527665083 2 46 -10.995736183056914 2 47 -11.363778054791048 2 48 -7.249918636611044 2 49 -11.901199780638368 2 50 -7.387831106710916 2 51 -7.246509749910647 2 52 -7.362637121738523 2 53 -7.620139066460913 2 54 -14.446111303478952 2 55 -6.667069728943202 2 56 -5.741534326674621 3 1 1.058285330877176 3 2 1.8635956641218243 3 3 1.7020246045202967 3 4 1.8397331573231028 3 5 1.7145827480646147 3 6 4.433117839547009 3 7 2.3380704918026254 3 8 2.621432244418422 3 9 3.521248758049161 3 10 2.5631101596117034 3 11 0.9029222995903581 3 12 2.2014545943543453 3 13 -7.4975993469196425 3 14 -7.233451760642669 3 15 -5.482780526453009 3 16 2.0774896888372325 3 17 2.6320877092807464 3 18 2.104285375895273 3 19 0.8019225714515805 3 20 0.43255073368077035 3 21 3.066115071018295 3 22 3.899833925420509 3 23 2.9650026207204476 3 24 3.606040208419735 3 25 3.155377866611161 3 26 2.567017816653774 3 27 2.7581119906097697 3 28 2.4655032365274465 3 29 3.2341541061467893 3 30 2.66401556748103 3 31 3.619607257820718 3 32 2.5378373632968163 3 33 1.9698705134631922 3 34 2.8360833901777553 3 35 2.9805659703311065 3 36 3.9367873523632824 3 37 3.549589806685038 3 38 4.8655981944770765 3 39 6.867101852757788 3 40 9.778762549494626 3 41 8.151577515478639 3 42 7.575674212555057 3 43 1.2953519500714883 3 44 1.8240413436304053 3 45 6.632103271009715 3 46 -0.789442476763206 3 47 -4.612029803042795 3 48 -0.6030654897578955 3 49 -9.576024955463543 3 50 -0.6780408969207059 3 51 -0.3339223373232336 3 52 -0.47976998887138933 3 53 -2.7092999056217515 3 54 -6.148908506276154 3 55 -1.7719648338383074 3 56 1.7357384005981054 4 1 1.3207684617813626 4 2 2.720208349681876 4 3 2.1534416085688832 4 4 2.821851915757648 4 5 1.8755139221536865 4 6 5.47596534292083 4 7 3.2425914094814345 4 8 3.2560476290338087 4 9 4.527996396375748 4 10 3.799277501042205 4 11 4.339494499320459 4 12 1.8306718683084569 4 13 5.216638169949455 4 14 3.5037277265368196 4 15 5.79049882577371 4 16 6.561632738769752 4 17 5.721156535191675 4 18 5.311437872521452 4 19 4.835660763084508 4 20 5.113724822749596 4 21 3.7445901047564902 4 22 4.361709768875301 4 23 3.1266085586421752 4 24 4.319940343372501 4 25 3.7822334671509736 4 26 3.0923214603784714 4 27 2.2881389811630797 4 28 2.6709688235719824 4 29 3.197716859183231 4 30 2.2513974838103152 4 31 2.6239932227329987 4 32 3.1096997114749527 4 33 1.5869420384294557 4 34 2.5486339974652026 4 35 1.9083662402366457 4 36 3.769445921863955 4 37 4.393719361340903 4 38 5.137528019038481 4 39 7.062783364228775 4 40 9.149882657456839 4 41 3.444762400768788 4 42 8.174527114039538 4 43 6.468428873148412 4 44 9.865539319338907 4 45 8.626030396515787 4 46 8.731137820132876 4 47 9.167322423677852 4 48 8.594977695127394 4 49 5.835243600541855 4 50 9.651243853416673 4 51 7.711624221381221 4 52 8.237841347160998 4 53 7.517420742151526 4 54 2.2620226678129143 4 55 8.809006826080722 4 56 7.0339840146331944 5 1 2.1917694937731014 5 2 3.1113332206829085 5 3 2.5383745291055178 5 4 3.1417693564181235 5 5 3.1139040150329436 5 6 5.407853887812472 5 7 3.7162756200077496 5 8 3.5305574329553764 5 9 4.402093403599693 5 10 4.0593394205468485 5 11 4.303374788278141 5 12 6.3941393605685235 5 13 6.60157109048609 5 14 6.797845373595639 5 15 6.926721735990434 5 16 7.753583203166038 5 17 7.404335069763398 5 18 7.124647366845501 5 19 5.892420309008141 5 20 5.856758878477152 5 21 3.8673971223003463 5 22 4.431885207471787 5 23 3.701427960086967 5 24 5.320972335116572 5 25 4.26623759511795 5 26 4.120185237468256 5 27 4.0435569378194245 5 28 3.8391834778547462 5 29 4.089357726056292 5 30 4.701345884223109 5 31 3.8303915715462082 5 32 5.2139308776256215 5 33 4.094681976509953 5 34 4.798375999529185 5 35 4.067292968822812 5 36 5.25654602506313 5 37 5.7765882983894095 5 38 6.2479511356535475 5 39 8.137086769801531 5 40 8.950708250852092 5 41 0.9566303058255485 5 42 10.069263956144798 5 43 12.52725240256018 5 44 12.634373168668116 5 45 10.264833286092667 5 46 11.835368986283552 5 47 10.174546365886311 5 48 11.905607210091276 5 49 11.437926779076427 5 50 11.33855035496466 5 51 10.597073137789891 5 52 9.971587477191957 5 53 10.028256655464219 5 54 9.931785309711778 5 55 10.160916010807242 5 56 10.14750310648046 6 1 1.9380930231848694 6 2 2.749813612839773 6 3 2.2773451173408077 6 4 2.915053670143614 6 5 2.953364799346666 6 6 5.284079378008549 6 7 3.2787756200077496 6 8 3.4018809623671444 6 9 4.035671834972241 6 10 3.3387511852527325 6 11 3.938178709846774 6 12 6.874531517431265 6 13 5.382208345388051 6 14 6.837061059870148 6 15 6.174270755598272 6 16 6.659220458067999 6 17 5.955805657998695 6 18 5.149157170767069 6 19 4.832371289400303 6 20 4.756268682398719 6 21 3.450730455633682 6 22 3.598551874138458 6 23 2.9281436463614767 6 24 3.957001746881275 6 25 2.9598650460983436 6 26 1.792979355115314 6 27 2.9920863495841274 6 28 2.59531092883514 6 29 3.327102824095512 6 30 3.114336080301541 6 31 2.098773924487382 6 32 2.911234799194247 6 33 1.4378192314119147 6 34 2.4883269799213394 6 35 1.599155713920851 6 36 0.7540950446709758 6 37 2.0560000630952935 6 38 2.9280981944770765 6 39 4.118704416860357 6 40 1.2595317802638597 6 41 2.0938852077863324 6 42 6.840097289478134 6 43 9.864262206481744 6 44 8.538784933373996 6 45 6.862872501778945 6 46 9.574339574518842 6 47 8.814252248239256 6 48 8.558793484601079 6 49 10.483269916331327 6 50 9.892471923592112 6 51 8.8519750985742 6 52 8.87599924189784 6 53 8.946148812326967 6 54 7.839873545005897 6 55 8.151112089238616 6 56 9.027405067264773 7 1 1.3839263565182023 7 2 1.7178691683953247 7 3 1.6704006728963634 7 4 2.0803314479213952 7 5 2.0380870215688915 7 6 4.5660238224529905 7 7 3.4718311755633096 7 8 2.9671587401449173 7 9 4.242616279416687 7 10 3.433195629697174 7 11 4.190956487624547 7 12 6.507864850764598 7 13 6.333597234276937 7 14 7.081505504314595 7 15 6.45482631115383 7 16 6.085609346956886 7 17 5.980805657998694 7 18 5.665823837433734 7 19 4.275426844955856 7 20 4.8646020157320535 7 21 2.334063788967015 7 22 3.3874407630273424 7 23 3.7628658685836953 7 24 3.1667239691035007 7 25 3.679309490542785 7 26 3.0665904662264225 7 27 3.089308571806349 7 28 2.9855887066129156 7 29 3.1521028240955076 7 30 3.071280524745987 7 31 2.5807183689318265 7 32 4.412623688083137 7 33 2.7600414536341376 7 34 3.448049202143565 7 35 2.6463779361430753 7 36 0.47353948911542076 7 37 1.5948889519841778 7 38 2.121153750032633 7 39 3.9867599724159106 7 40 -1.5682459975139196 7 41 -11.061670347769223 7 42 5.535930622811467 7 43 7.9795399842595245 7 44 7.741562711151772 7 45 5.851761390667832 7 46 8.967395130074397 7 47 9.761474470461476 7 48 8.765737929045521 7 49 10.75688102744244 7 50 8.92024970136989 7 51 9.54086398746309 7 52 9.678777019675618 7 53 9.719759923438076 7 54 10.869040211672562 7 55 10.184445422571947 7 56 9.157960622820326 8 1 2.005695064001195 8 2 2.7800006876697054 8 3 2.8236886547557773 8 4 2.272621697354502 8 5 2.5736879285983676 8 6 5.1093344800493625 8 7 3.8455273206880207 8 8 3.378496608625646 8 9 4.843069794155916 8 10 3.9492954029398035 8 11 4.92882496835017 8 12 6.823511109267997 8 13 7.363075692326824 8 14 7.639782148305525 8 15 6.938726537911201 8 16 6.168149029496568 8 17 6.635227426706173 8 18 6.75970138845414 8 19 4.798782854026154 8 20 5.06324147151436 8 21 3.5884855576744954 8 22 4.561136908152058 8 23 4.060371537517938 8 24 4.226134399942502 8 25 4.685205182152764 8 26 3.845275273482663 8 27 2.261644172713382 8 28 1.9792395002637093 8 29 3.1085654091295254 8 30 3.127516352410389 8 31 2.8900154210860265 8 32 2.9346191529357455 8 33 2.0942818164459283 8 34 1.7591603132546751 8 35 2.1901421084786747 8 36 0.4046052487526097 8 37 0.03389121955787644 8 38 -0.12419772389026917 8 39 2.4244016957719197 8 40 -1.6027131176953233 8 41 -10.620400506499381 8 42 4.055658513967931 8 43 6.774551322128005 8 44 6.639975409564471 8 45 4.11797454259527 8 46 6.243132091525645 8 47 6.599116193817487 8 48 7.332177838342574 8 49 9.99815086871228 8 50 7.598254236517285 8 51 9.083267615581008 8 52 9.705080874550905 8 53 9.103886907565062 8 54 9.327543613033107 8 55 10.38580596678964 8 56 9.103085339373617 9 1 1.7982120708039204 9 2 2.7289802795064375 9 3 2.8577022601979536 9 4 2.912077479667424 9 5 2.730150513632381 9 6 5.65015080657998 9 7 3.64484704857918 9 8 3.6846190576052393 9 9 4.741028977829387 9 10 3.5411321376336815 9 11 4.932226328894387 9 12 6.636436279336024 9 13 6.8256607263404305 9 14 8.289442012251104 9 15 7.370699327026848 9 16 7.168149029496568 9 17 7.128424705617739 9 18 6.827728599338492 9 19 6.424633194162208 9 20 4.607459158589194 9 21 2.8912066461098718 9 22 4.360456636043217 9 23 3.0739769796948053 9 24 4.73973984211937 9 25 3.0253412365745334 9 26 3.855479355115314 9 27 3.843276825774609 9 28 2.3840014050256144 9 29 4.094959966952651 9 30 3.5458836993491616 9 31 4.7505596387730975 9 32 5.509449084908532 9 33 4.6282954218881045 9 34 3.497255551349916 9 35 4.533679523444661 9 36 2.7243331399090707 9 37 3.4964762535714833 9 38 4.556074384953266 9 39 5.145490131146072 9 40 -0.15713488640280815 9 41 -1.7394481255469998 9 42 7.974025860906707 9 43 10.801762206481744 9 44 10.568546838135898 9 45 7.529539168445613 9 46 9.535649098328367 9 47 9.391633200620209 9 48 9.740341103648696 9 49 11.521960392521805 9 50 10.380567161687352 9 51 8.328165574764682 9 52 8.286713527612125 9 53 8.889601193279347 9 54 9.830944973577324 9 55 10.032064470190996 9 56 8.592881257740963 10 1 1.2283708009626437 10 2 2.4289802795064404 10 3 1.514845117340812 10 4 1.7469981145880595 10 5 1.6158647993466673 10 6 4.521579378008546 10 7 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1.2539647749889866 115 29 2.464923336916023 115 30 1.9524771059425703 115 31 0.4465303347437981 115 32 -4.250624175164724 115 33 0.9121782057708856 115 34 1.1053141594085218 115 35 1.21133520110034 115 36 3.321402736978669 115 37 3.2034359605311913 115 38 4.8655981944770765 115 39 6.020948006603941 115 40 8.240301011033093 115 41 8.632346746247872 115 42 5.633366520247364 115 43 5.853044257763795 115 44 4.189425959015022 115 45 5.747487886394328 115 46 3.1144036770829473 115 47 -1.2274144184274114 115 48 -1.545373182065589 115 49 -2.749101878540465 115 50 4.0142667953869875 115 51 4.83915458575369 115 52 4.347153088051687 115 53 3.867623171301325 115 54 3.812629955262306 115 55 4.497265935392463 115 56 5.620353785213489 116 1 1.5505930231848666 116 2 1.0289802795064347 116 3 0.7148451173408077 116 4 1.5692203368102824 116 5 1.0825314660133358 116 6 3.488246044675215 116 7 1.7162756200077496 116 8 1.922714295700473 116 9 2.0981718349722414 116 10 0.45541785191939965 116 11 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-0.983749396039066 119 34 -1.1820161573335568 119 35 -0.7954521292164003 119 36 1.7467421034945048 119 37 1.6197255532913708 119 38 2.8950099591829606 119 39 4.333165201174079 119 40 7.891884721440331 119 41 4.917414619551039 119 42 -1.7738732987571595 119 43 -1.4139240680280594 119 44 1.2618241490602706 119 45 -0.5194804393975261 119 46 -7.184238856853707 119 47 -7.668590889015647 119 48 -10.192432005595 119 49 -6.052269299354947 119 50 -5.347724154839259 119 51 -3.63822097985717 119 52 -5.263706640455101 119 53 -6.677625697476955 119 54 -8.087822533425477 119 55 -6.701829087231973 119 56 -4.676026305284246 120 1 0.8592886753587834 120 2 1.533328105593391 120 3 1.2220914941524015 120 4 1.4793652643465123 120 5 1.7839807413756503 120 6 3.6781011171389864 120 7 1.5713480837758667 120 8 1.603873715990332 120 9 1.5112153132331088 120 10 0.5162874171367875 120 11 -9.781024188703952 120 12 -0.9298163086556919 120 13 -4.424856872003254 120 14 -8.655692563318254 120 15 -3.889135041503174 120 16 -8.968496933236349 120 17 -9.013397240552031 120 18 -2.2820022495227903 120 19 -1.2047663917591187 120 20 -3.332499433543312 120 21 0.3311652382423773 120 22 1.3739141929790364 120 23 1.0594842260716177 120 24 -1.159846079205682 120 25 1.458053451895445 120 26 2.138088050767486 120 27 2.809115335091377 120 28 2.5382457114438353 120 29 2.1405086211969575 120 30 3.0210389788522676 120 31 -1.130392742179282 120 32 -8.942931867472417 120 33 -0.004209754095334972 120 34 -4.153883165006196 120 35 -1.09468486578929 120 36 1.6290950446709758 120 37 -0.15776805284673756 120 38 -0.3735322403055328 120 39 0.57613195309224 120 40 0.40083612808994573 120 41 3.5286678164819847 120 42 -2.5539244496522997 120 43 -2.8793972138081116 120 44 -6.415925211553541 120 45 -8.724084019960186 120 46 -13.480914048669561 120 47 -10.64045789668828 120 48 -10.414938399456894 120 49 -15.15712863439331 120 50 -9.567673003944117 120 51 -9.29295243765768 120 52 -10.412044236363029 120 53 -11.324684521006368 120 54 -12.829510512965118 120 55 -11.044540084674427 120 56 -8.003392034184504 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 288.0 5 65.0 6 69.0 7 44.0 8 60.0 9 44.0 10 33.0 11 41.0 12 37.0 13 55.0 14 81.0 15 129.0 16 192.0 17 237.0 18 257.0 19 355.0 20 441.0 21 577.0 22 687.0 23 803.0 24 941.0 25 962.0 26 1004.0 27 1035.0 28 1187.0 29 1175.0 30 1358.0 31 1539.0 32 1853.0 33 2172.0 34 2548.0 35 3119.0 36 3331.0 37 3124.0 38 1692.0 39 472.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 100.0 0.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 99.20642359483864 0.6623551098197269 0.04686474833630143 0.08435654700534258 8 99.33139625706877 0.5155122316993158 0.10622676289561658 0.04686474833630143 9 98.07818505671698 1.2280866222930533 0.21249335958251303 0.481234961407456 10 46.95222919986253 37.65113881338457 7.654575561595902 7.742056425156997 11 28.61026057614197 23.689308254702244 30.378678997687935 17.32175217146785 12 43.31864904552129 12.906551691817414 18.97085012653482 24.803949136126473 13 22.02955603461743 24.39153935076702 21.370325241353456 32.2085793732621 14 34.09268460360614 15.762007437267586 21.736820724352366 28.408487234773915 15 19.844126576731664 29.28730163698394 19.850386553569752 31.01818523271464 16 24.97578352029497 18.557635221697964 33.41874199293816 23.0478392650689 17 39.79001968565447 20.973033778083305 17.27025591350811 21.96669062275412 18 37.93200424973439 25.867133304168487 16.967689519405038 19.23317292669208 19 24.73514797337417 39.635613612925404 15.453607925247665 20.175630488452764 20 23.989749038972406 25.177360377535397 17.154733256242775 33.67815732724943 21 23.797768959160077 24.32896915914133 32.525075774146174 19.34818610755242 22 21.682756896928797 25.30383978504702 28.749960946043053 24.263442371981128 23 23.63545474427469 39.49448558127909 18.677164370293998 18.192895304152216 24 21.573405817477425 39.431999250164026 18.171025088261946 20.823569844096603 25 21.662240274957036 39.809404780503044 18.872051241993436 19.656303702546477 26 27.679744696827484 26.43764470308491 26.537763594268192 19.34484700581941 27 33.2166630207194 23.08509640926279 22.291321603800117 21.406918966217695 28 18.421052631578945 38.58501783590963 23.06464734964641 19.92928218286501 29 18.651537884471118 39.034758689672415 25.340710177544384 16.97299324831208 30 19.04479104804176 24.599131059919358 40.70265370549808 15.653424186540807 31 18.32409928944815 25.454659279431556 41.305912918270884 14.915328512849408 32 33.303125 22.14375 26.828125000000004 17.724999999999998 33 20.146497214048704 36.34257810054467 27.7624741751706 15.748450510236022 34 17.579530169852045 25.07429071913416 30.811098251431073 26.53508085958272 35 30.379192847541344 25.755728531682763 30.30104098283785 13.564037637938043 36 29.485935984481088 26.316448171208663 29.520352930133598 14.677262914176653 37 29.543318925014095 26.31710831297375 30.7492326003884 13.390340161623755 38 18.790097956373423 26.98025224548556 40.70666291115075 13.522986886990267 39 16.41678690170002 32.717520858164484 26.861551972900067 24.004140267235428 40 11.572327044025158 16.849056603773587 21.72327044025157 49.855345911949684 41 20.74870695092721 18.689289769143432 24.239939447458056 36.3220638324713 42 15.320761809297082 26.534895376519234 31.080065154742513 27.06427765944117 43 9.769177695540362 20.617198770620334 24.725584896192686 44.888038637646616 44 11.334862241211399 17.043134051710595 23.181803901856682 48.440199805221326 45 16.887261804186615 36.52179354798336 30.739384836822182 15.851559811007853 46 27.454107639866155 25.602776995965847 31.760327735559933 15.182787628608063 47 14.04194143200925 23.43032159264931 40.92571178547989 21.60202518986155 48 15.432947796186308 25.739293529227886 43.56048765239137 15.267271022194437 49 11.31696918398696 17.304617699614408 22.919213768456693 48.45919934794194 50 14.964452378715274 25.105703279150614 45.03429484167998 14.895549500454134 51 27.79293261844292 26.836609914401276 30.533345875270435 14.837111591885366 52 15.194202171048504 39.411432515529896 29.98682311601933 15.407542197402272 53 27.414808318717114 26.600476071160113 30.87258832372839 15.112127286394387 54 14.719319106327056 23.02396895925903 38.015520370486264 24.241191563927657 55 16.39996242131964 25.5253186358939 31.531644380421508 26.54307456236495 56 28.413931345527438 25.394637935354545 30.503006765221745 15.688423953896265 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 1.0 10 2.0 11 3.0 12 8.5 13 23.0 14 32.0 15 77.5 16 123.0 17 195.0 18 267.0 19 320.5 20 374.0 21 413.0 22 465.0 23 478.0 24 489.5 25 501.0 26 561.0 27 621.0 28 705.5 29 868.5 30 947.0 31 1016.0 32 1085.0 33 1082.5 34 1080.0 35 1028.5 36 977.0 37 902.5 38 737.0 39 646.0 40 607.0 41 568.0 42 551.5 43 535.0 44 535.5 45 536.0 46 545.0 47 663.5 48 773.0 49 1063.5 50 1354.0 51 1530.5 52 1707.0 53 1620.5 54 1460.5 55 1387.0 56 1425.5 57 1464.0 58 1485.5 59 1507.0 60 1513.0 61 1519.0 62 1576.0 63 1675.0 64 1717.0 65 1647.5 66 1578.0 67 1456.0 68 1334.0 69 1156.5 70 979.0 71 850.5 72 624.0 73 526.0 74 435.5 75 345.0 76 291.5 77 238.0 78 187.5 79 98.5 80 60.0 81 52.0 82 44.0 83 33.0 84 22.0 85 15.5 86 9.0 87 7.0 88 2.5 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.018745899334520574 10 0.0 11 0.0031243165557534288 12 0.0 13 0.0 14 0.018745899334520574 15 0.18121036023369888 16 0.012497266223013715 17 0.012497266223013715 18 0.015621582778767143 19 0.02499453244602743 20 0.031243165557534286 21 0.012497266223013715 22 0.0 23 0.0 24 0.0 25 0.0062486331115068575 26 0.1405942450089043 27 0.02499453244602743 28 0.153091511231918 29 0.04686474833630143 30 0.043740431780548004 31 0.18745899334520572 32 0.021870215890274002 33 0.19058330990095915 34 0.1187240291186303 35 0.056237698003561716 36 0.14371856156465773 37 0.25306964101602775 38 0.16871309401068516 39 0.3905395694691786 40 0.6467335270409598 41 0.9341706501702752 42 0.2593182741275346 43 0.3780423032461649 44 0.5498797138126035 45 0.1499671946761646 46 0.09372949667260286 47 0.031243165557534286 48 0.05311338144780829 49 0.3374261880213703 50 0.24369669134876745 51 0.3561720873558909 52 0.41553410191520607 53 0.24682100790452088 54 0.153091511231918 55 0.23119942512575373 56 0.24682100790452088 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 32007.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.04411534976724 #Duplication Level Percentage of deduplicated Percentage of total 1 93.52044609665427 78.59843159308902 2 4.360594795539034 7.329646639797545 3 1.0074349442379182 2.5400693598275375 4 0.3754646840148699 1.2622238885243853 5 0.20446096654275092 0.859187052832193 6 0.11152416356877323 0.5623769800356172 7 0.08550185873605948 0.5030149654763021 8 0.04460966542750929 0.2999343893523292 9 0.03345724907063197 0.25306964101602775 >10 0.20446096654275092 3.2961539663198676 >50 0.029739776951672865 1.8152279188927423 >100 0.022304832713754646 2.6806636048364423 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGCGGAGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG 167 0.5217608648108226 No Hit TGCGGAGGGATGAGATGGACTTAAATGAATTGACGGGTACCCACAGTTCTGAGTCG 162 0.5061392820320555 No Hit TGCGGAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG 140 0.43740431780548 No Hit TGCGGAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG 137 0.4280313681382198 No Hit TGCGGAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG 133 0.41553410191520607 No Hit TGCGGAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG 119 0.371793670134658 No Hit TGCGGAGGGATGAGATGGACTCAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG 97 0.3030587059080826 No Hit TGCGGAGGGATGAGATGGACTCAAATGAATTGACGGGTACCCACAGTTCTGAGTCG 94 0.2936857562408223 No Hit TGCGGAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG 76 0.2374480582372606 No Hit TGCGGAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG 74 0.23119942512575373 No Hit TGCGGAGGGATGAGATGGACTCAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG 65 0.2030805761239729 No Hit TGCGGAGGGATGCGCTGGACTTAAAGGAATTGACGGGTCCGCCCAGTTCTGAGTCG 61 0.19058330990095915 No Hit TGCGGAGGGATGCGCTGGACTTAAATGAATTGACGGGTCCGCCCAGTTCTGAGTCG 58 0.18121036023369888 No Hit TGCGGAGGGATGAGATGGACTCAAATGAATTGACGGGTACGCACAGTTCTGAGTCG 56 0.17496172712219202 No Hit TGCGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA 44 0.13746992845315087 No Hit TGCGGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA 41 0.12809697878589058 No Hit TGCGGAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCCTGCGCTT 34 0.10622676289561658 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0031243165557534288 0.0 0.0 0.0 0.0 7 0.0031243165557534288 0.0 0.0 0.0 0.0 8 0.0031243165557534288 0.0 0.0 0.0 0.0 9 0.0031243165557534288 0.0 0.0 0.0 0.0 10 0.0062486331115068575 0.0 0.0 0.0 0.0 11 0.009372949667260287 0.0 0.0 0.0 0.0 12 0.012497266223013715 0.0 0.0 0.0 0.0 13 0.015621582778767145 0.0 0.0 0.0 0.0 14 0.015621582778767145 0.0 0.0 0.0 0.0 15 0.018745899334520574 0.0 0.0 0.0 0.0 16 0.02499453244602743 0.0 0.0 0.0 0.0 17 0.02499453244602743 0.0 0.0 0.0 0.0 18 0.03124316555753429 0.0 0.0 0.0 0.0 19 0.03436748211328772 0.0 0.0 0.0 0.0 20 0.03436748211328772 0.0 0.0 0.0 0.0 21 0.03436748211328772 0.0 0.0 0.0 0.0 22 0.03749179866904115 0.0 0.0 0.0 0.0 23 0.04061611522479457 0.0 0.0 0.0 0.0 24 0.04061611522479457 0.0 0.0 0.0 0.0 25 0.04061611522479457 0.0 0.0 0.0 0.0 26 0.043740431780548004 0.0 0.0 0.0 0.0 27 0.04998906489205486 0.0 0.0 0.0 0.0 28 0.05311338144780829 0.0 0.0 0.0 0.0 29 0.06248633111506858 0.0 0.0 0.0 0.0 30 0.065610647670822 0.0 0.0 0.0 0.0 31 0.06873496422657543 0.0 0.0 0.0 0.0 32 0.06873496422657543 0.0 0.0 0.0 0.0 33 0.06873496422657543 0.0 0.0 0.0 0.0 34 0.06873496422657543 0.0 0.0 0.0 0.0 35 0.07185928078232887 0.0 0.0 0.0 0.0 36 0.07185928078232887 0.0 0.0 0.0 0.0 37 0.07185928078232887 0.0 0.0 0.0 0.0 38 0.07185928078232887 0.0 0.0 0.0 0.0 39 0.07185928078232887 0.0 0.0 0.0 0.0 40 0.07185928078232887 0.0 0.0 0.0 0.0 41 0.07185928078232887 0.0 0.0 0.0 0.0 42 0.07185928078232887 0.0 0.0 0.0 0.0 43 0.07185928078232887 0.0 0.0 0.0 0.0 44 0.07185928078232887 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCCTAT 15 0.007945813 50.639553 38 TCCGGCC 15 0.007945813 50.639553 38 GCCCTTG 15 0.007945813 50.639553 38 TCCGCCC 70 0.0 50.63955 38 CACTGAC 15 0.007996145 50.558918 41 TCAAGAC 15 0.008046714 50.47854 34 GTCAACA 15 0.008046714 50.47854 36 CGGTCAA 15 0.008046714 50.47854 34 CGATCCT 20 3.9324068E-4 50.47854 36 TGCAATC 20 4.0261226E-4 50.238926 50 ACGGGTC 100 0.0 50.238926 33 ACTGACT 15 0.008199846 50.238922 42 TTCGATG 15 0.008199846 50.238922 50 GCCAGTT 15 0.008199846 50.238922 42 GCTGAGT 20 4.0577524E-4 50.15956 48 GTGCAAT 15 0.008251369 50.159557 49 ACTTTCG 15 0.008251369 50.159557 47 CGTGCGC 15 0.008251369 50.159557 48 CGTGCAA 15 0.008251369 50.159557 48 GCTCTGA 25 2.0032676E-5 50.159557 46 >>END_MODULE