FastQCFastQC Report
Fri 17 Jun 2016
SRR1526308_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526308_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42992
Sequences flagged as poor quality0
Sequence length56
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCAGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG2180.5070710829921845No Hit
TGATCAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG2030.47218087085969485No Hit
TGATCAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG1830.4256605880163751No Hit
TGATCAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG1800.41868254558987716No Hit
TGATCAGGGATGAGATGGACTTAAATGAATTGACGGGTACCCACAGTTCTGAGTCG1760.40937848902121327No Hit
TGATCAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG1590.3698362486043915No Hit
TGATCAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC1140.2651656122069222No Hit
TGATCAGGGATGAGATGGACTCAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG1010.23492742835876443No Hit
TGATCAGGGATGAGATGGACTCAAATGAATTGACGGGTACCCACAGTTCTGAGTCG930.21631931522143655No Hit
TGATCAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG840.1953851879419427No Hit
TGATCAGGGATGAGATGGACTCAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG780.18142910308894677No Hit
TGATCAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG780.18142910308894677No Hit
TGATCAGGGATGCGCTGGACTTAAATGAATTGACGGGTCCGCCCAGTTCTGAGTCG710.1651470040937849No Hit
TGATCAGGGATGCGCTGGACTTAAAGGAATTGACGGGTCCGCCCAGTTCTGAGTCG690.16049497580945293No Hit
TGATCAGGGATGAGATGGACTCAAATGAATTGACGGGTACGCACAGTTCTGAGTCG660.15351693338295497No Hit
TGATCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC480.11164867882396724No Hit
TGATCAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCGAACCTGCGTGCAATC450.10467063639746929No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAAAA203.884566E-450.64412739
ACCTGTG150.00796192850.64412740
ACACAGT150.00796192850.64412741
ACTTCCA150.00796192850.64412739
AACTAAA203.884566E-450.64412738
AGACCCT150.00796192850.64412740
GACCTGT150.00796192850.64412739
TTAATTA150.00796192850.64412741
TACTCAC150.00796192850.64412738
CCGACCA150.00796192850.64412739
GAGACCC150.00796192850.64412739
AAACTAA203.9074113E-450.58412637
ATACGCC203.9074113E-450.58412637
GTCCGAC150.00799939350.5841237
GTACACA150.00799939350.5841237
CGGCCAA251.9168287E-550.5841237
GGTCCGA150.00811257650.4049636
GGTACTC150.00811257650.4049636
GATACGC251.9576091E-550.40495736
GGATACG251.9713612E-550.3455235