##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526308_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 42992 Sequences flagged as poor quality 0 Sequence length 56 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.22885653144771 39.0 37.0 39.0 33.0 40.0 2 36.41321641235579 39.0 36.0 39.0 30.0 40.0 3 35.00753628582062 38.0 35.0 39.0 25.0 40.0 4 36.68135932266468 39.0 36.0 39.0 30.0 40.0 5 36.628419240788986 39.0 36.0 39.0 30.0 40.0 6 33.91926404912542 38.0 33.0 39.0 17.0 40.0 7 36.137281354670634 38.0 35.0 39.0 30.0 40.0 8 36.10078619278005 38.0 35.0 39.0 30.0 40.0 9 36.062686081131375 38.0 35.0 39.0 29.0 40.0 10 35.77451618905843 38.0 35.0 39.0 29.0 40.0 11 34.66412355787123 38.0 33.0 39.0 26.0 40.0 12 32.54784611090435 36.0 29.0 39.0 18.0 40.0 13 31.820408448083363 36.0 28.0 39.0 15.0 40.0 14 31.392468366207666 36.0 27.0 39.0 13.0 40.0 15 32.06308150353554 36.0 29.0 39.0 17.0 40.0 16 32.41703107554894 36.0 30.0 39.0 18.0 40.0 17 32.71178358764421 36.0 30.0 39.0 18.0 40.0 18 32.643538332713064 36.0 30.0 39.0 19.0 40.0 19 33.58943524376628 36.0 31.0 39.0 24.0 40.0 20 33.89793449944175 37.0 31.0 39.0 25.0 40.0 21 35.36022981019725 38.0 35.0 39.0 27.0 40.0 22 35.03365742463714 38.0 35.0 39.0 26.0 40.0 23 35.107368812802385 38.0 35.0 39.0 27.0 40.0 24 34.66975251209527 38.0 34.0 39.0 25.0 40.0 25 34.99034704131001 38.0 35.0 39.0 27.0 40.0 26 35.33694640863416 38.0 35.0 39.0 28.0 40.0 27 35.410820617789355 38.0 35.0 39.0 28.0 40.0 28 35.19936267212505 38.0 35.0 39.0 28.0 40.0 29 35.15093505768515 38.0 35.0 39.0 27.0 40.0 30 34.84957666542613 38.0 35.0 39.0 26.0 40.0 31 34.80070710829922 38.0 35.0 39.0 26.0 40.0 32 33.6455154447339 38.0 33.0 39.0 19.0 40.0 33 34.91256512839598 38.0 35.0 39.0 27.0 40.0 34 34.78088946780797 38.0 35.0 39.0 26.0 40.0 35 34.85297264607369 38.0 35.0 39.0 27.0 40.0 36 32.69596669147749 36.0 31.0 38.0 23.0 39.0 37 32.43540658727205 36.0 31.0 38.0 22.0 39.0 38 31.239695757350205 35.0 30.0 38.0 13.0 39.0 39 29.716854298474136 35.0 28.0 38.0 5.0 39.0 40 25.739556196501674 32.0 12.0 38.0 4.0 39.0 41 21.88551358392259 25.0 9.0 33.0 4.0 37.0 42 25.72727484183104 30.0 20.0 34.0 4.0 37.0 43 22.35285634536658 27.0 9.0 33.0 4.0 37.0 44 23.399516189058428 28.0 10.0 33.0 4.0 37.0 45 25.676358392259026 30.0 21.0 34.0 4.0 38.0 46 26.079805545217717 31.0 18.0 37.0 4.0 38.0 47 25.17093877930778 30.0 11.0 36.0 4.0 38.0 48 25.268142910308896 30.0 14.0 36.0 4.0 38.0 49 22.72915891328619 28.0 5.0 35.0 4.0 38.0 50 25.117324153330852 30.0 13.0 36.0 4.0 38.0 51 26.43791868254559 33.0 4.0 38.0 4.0 40.0 52 26.274748790472646 33.0 4.0 38.0 4.0 40.0 53 25.86448641607741 32.0 4.0 38.0 4.0 40.0 54 24.95755024190547 32.0 4.0 38.0 4.0 39.0 55 25.271608671380722 32.0 4.0 38.0 4.0 40.0 56 24.663751395608486 32.0 4.0 38.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.5740689810191313 1 2 2.5360086988118624 1 3 3.674039676364181 1 4 2.243423750436314 1 5 2.227818733775642 1 6 6.073428680140875 1 7 2.8893701503834635 1 8 3.022710777803752 1 9 3.1573902797660196 1 10 2.89327974424949 1 11 -3.161546537906279 1 12 -6.8870366604976105 1 13 -21.528214393719516 1 14 -17.548267058148106 1 15 -11.254630238713776 1 16 -21.4567152460392 1 17 -21.479328701176172 1 18 -21.0933744865148 1 19 -5.0029144482116905 1 20 -10.336933992267593 1 21 -8.549131768070563 1 22 -3.107797944673912 1 23 -2.7881054439662165 1 24 -3.2324475061463467 1 25 -3.703344128344977 1 26 3.3352093612309517 1 27 2.719600874877031 1 28 2.7594604274121934 1 29 2.6554087691005037 1 30 2.9374549260815357 1 31 2.2162527888772203 1 32 -12.687847780890731 1 33 1.6220650378430719 1 34 1.2189299055201062 1 35 1.415574974600439 1 36 4.942330684357913 1 37 5.176262365679342 1 38 5.861208663813493 1 39 7.8970322176108745 1 40 10.754365466640131 1 41 -1.3703685143043458 1 42 -6.4322446155641835 1 43 -14.47446660780688 1 44 -16.51653119768395 1 45 -14.869163857526733 1 46 -18.71704923068283 1 47 -18.143085874352046 1 48 -15.990620917442044 1 49 -15.306094123139545 1 50 -16.018013513785412 1 51 -16.818103727039173 1 52 -16.70005222051586 1 53 -17.740357972087157 1 54 -18.350532809161695 1 55 -17.337764899901405 1 56 -16.759382315069754 2 1 1.9200338932998307 2 2 2.826885891794319 2 3 4.266320378118564 2 4 2.476757083769648 2 5 2.6060643478107295 2 6 6.050972539789999 2 7 3.017089448629079 2 8 3.290780953242347 2 9 3.1398464201168963 2 10 3.3357358846003677 2 11 -1.4717219765027671 2 12 -8.38142262540989 2 13 -10.546460007754607 2 14 -12.971424952884949 2 15 -5.470068835205005 2 16 -2.5440836670918294 2 17 -0.8891532625796827 2 18 -8.128462205813047 2 19 -0.2958969043520412 2 20 -2.3137760975307486 2 21 3.1715699863154043 2 22 3.6953599500629295 2 23 3.4876840297179967 2 24 3.466850739467688 2 25 3.3166558716550227 2 26 3.191349712108142 2 27 2.5518815766314162 2 28 3.1605130589911354 2 29 3.4968122778724364 2 30 3.503068961169255 2 31 1.2860773502807277 2 32 -6.575917956329327 2 33 0.6532931080185094 2 34 0.8775263967481735 2 35 1.1327679570565792 2 36 5.534611386112296 2 37 5.843630786731971 2 38 7.10050690942753 2 39 7.889312919365256 2 40 10.921032133306795 2 41 8.707175345344776 2 42 5.036878191453361 2 43 0.4016737430703117 2 44 -6.788461022245354 2 45 3.368029124929407 2 46 -8.138101862261776 2 47 -7.743085874352047 2 48 -4.389919163056078 2 49 -9.266445000332528 2 50 -4.840820531329271 2 51 -4.584770393705838 2 52 -5.01584169420007 2 53 -4.418252708929263 2 54 -12.88141000214415 2 55 -5.3044315665680735 2 56 -4.580084069455719 3 1 1.4471124592799995 3 2 1.7078927567828757 3 3 3.383025183610556 3 4 1.766612156233414 3 5 2.104920183051 3 6 5.859515636662618 3 7 2.769949860528392 3 8 2.7003919372240404 3 9 2.8675352073022538 3 10 2.0489319181625376 3 11 1.2071491142676365 3 12 2.517890875734274 3 13 -4.726475263284737 3 14 -5.374933724814774 3 15 -3.4105722676992833 3 16 2.8380673626564494 3 17 2.5769031828817965 3 18 2.022567542470707 3 19 0.6941870010636677 3 20 0.4085732541092213 3 21 2.41260736236422 3 22 3.185825243731884 3 23 3.3168220922656673 3 24 3.950451044578294 3 25 2.954337031075312 3 26 1.9381079119555906 3 27 2.4077168169060172 3 28 2.217721296977409 3 29 2.4136696386657235 3 30 2.1270201434728406 3 31 2.182339745398963 3 32 1.0724420741817298 3 33 1.2200360523358214 3 34 2.149074833056339 3 35 2.4584735253250827 3 36 4.288997351024577 3 37 4.823798597563396 3 38 5.192802866712043 3 39 6.83210468137899 3 40 10.341321988379264 3 41 10.080935833521739 3 42 7.677610456899586 3 43 3.2353884646568893 3 44 2.5776717008667767 3 45 7.563299910589208 3 46 0.48584932004180814 3 47 -3.554680077250598 3 48 0.9960457492246242 3 49 -10.386963688356936 3 50 0.8906821383885024 3 51 0.6471136642651771 3 52 -0.38121164080571646 3 53 -1.2168797112175902 3 54 -5.371112519306621 3 55 -0.8986344651187963 3 56 2.802066960292567 4 1 2.588863501567076 4 2 3.366419657715973 4 3 4.449930087323082 4 4 3.1297251202993266 4 5 3.225626952953725 4 6 6.964935529455946 4 7 3.6006030270957936 4 8 3.7339436545160822 4 9 3.6916368551084844 4 10 3.9834167305508643 4 11 4.83133017442249 4 12 4.770223613474997 4 13 6.5326075240887 4 14 4.894472667879288 4 15 7.0204382544369075 4 16 6.896435438892308 4 17 6.794095956358074 4 18 6.673748801156432 4 19 5.417633496993787 4 20 5.053202994033782 4 21 4.411416177134917 4 22 4.377407534778143 4 23 4.44586715877351 4 24 4.6379634527577664 4 25 4.111176419600227 4 26 3.0228805941076615 4 27 2.5026145735071665 4 28 2.438090564398493 4 29 3.0312991800594062 4 30 2.6410165699171557 4 31 3.520636350521059 4 32 3.5151659177394023 4 33 2.819599284418416 4 34 3.5211216863420205 4 35 2.830917440353865 4 36 4.978495067919553 4 37 5.581193872528658 4 38 6.0798388007997985 4 39 8.223607560076626 4 40 10.788612041982596 4 41 4.388261622681956 4 42 8.319262233750884 4 43 6.452382707261613 4 44 11.709496199576325 4 45 9.06617860822669 4 46 8.954183646029502 4 47 8.57198261879864 4 48 8.822529767489463 4 49 5.325960671381004 4 50 8.788835801283081 4 51 7.807923670221104 4 52 7.601317642497836 4 53 7.451970795036132 4 54 1.4420699305643332 4 55 8.170454278180785 4 56 7.714316315067233 5 1 2.641761288711443 5 2 2.9837010065041696 5 3 4.107885830210336 5 4 3.174192981205543 5 5 3.4770495030064126 5 6 6.956505603217796 5 7 3.4062932273065414 5 8 3.9242492393422097 5 9 3.5266210489967875 5 10 3.4763566673264137 5 11 4.813838077478337 5 12 7.065271031810084 5 13 6.844093298588174 5 14 7.845579095698046 5 15 7.5976774535939136 5 16 8.034053984730033 5 17 6.67144052959306 5 18 6.93739474425443 5 19 5.927854782557539 5 20 5.961527546193949 5 21 4.398560539621748 5 22 4.001432824556858 5 23 4.49497147911071 5 24 4.716783263084423 5 25 3.8089635639627133 5 26 3.0736708996924875 5 27 2.4703701056462606 5 28 3.293306581258342 5 29 3.196947230638969 5 30 3.3036087722353855 5 31 3.5562527888772237 5 32 4.7444599114169606 5 33 3.531295807073839 5 34 4.14662221321241 5 35 2.994036513061978 5 36 4.653099915127143 5 37 4.573185442602416 5 38 6.442747125351957 5 39 7.284724525303179 5 40 7.638980851255518 5 41 1.5357853318494996 5 42 10.295447692128125 5 43 12.91476416142389 5 44 12.245007263854514 5 45 10.506220757857886 5 46 11.17525846162486 5 47 12.518452587186417 5 48 12.467840621019498 5 49 13.043136646091227 5 50 12.066601870829974 5 51 11.374203965268524 5 52 10.40764008717645 5 53 10.770411258682074 5 54 11.054082575453688 5 55 11.839158177021673 5 56 12.131386915699476 6 1 1.9403189810191321 6 2 2.77288369881186 6 3 3.572789676364181 6 4 2.6121737504363125 6 5 3.0265687337756404 6 6 6.549053680140876 6 7 3.4606201503834626 6 8 3.1564607778037512 6 9 3.5792652797660196 6 10 3.384529744249491 6 11 4.4734534620937225 6 12 7.216713339502391 6 13 7.017410606280482 6 14 7.791732941851894 6 15 7.627244761286224 6 16 6.684534753960801 6 17 6.650046298823828 6 18 6.562875513485199 6 19 4.631460551788308 6 20 5.110566007732409 6 21 3.3439932319294385 6 22 3.916577055326087 6 23 3.2187695560337843 6 24 3.6381774938536537 6 25 4.097905871655023 6 26 3.2820843612309503 6 27 2.6239758748770328 6 28 2.7863354274121903 6 29 3.029158769100505 6 30 2.366829926081536 6 31 3.356252788877221 6 32 4.152152219109269 6 33 2.912065037843071 6 34 3.610804905520105 6 35 3.5055749746004423 6 36 3.5754556843579124 6 37 3.740012365679341 6 38 5.146833663813496 6 39 6.758282217610873 6 40 5.238740466640131 6 41 5.080256485695653 6 42 8.356505384435817 6 43 11.24240839219312 6 44 10.88971880231605 6 45 8.990836142473267 6 46 10.454825769317171 6 47 10.975664125647953 6 48 10.548129082557956 6 49 11.748905876860455 6 50 9.500736486214588 6 51 8.14439627296083 6 52 8.581197779484139 6 53 9.411517027912844 6 54 9.063217190838305 6 55 6.893485100098594 6 56 5.966242684930247 7 1 2.734068981019135 7 2 3.0260086988118573 7 3 3.4373730096975166 7 4 3.0100904171029796 7 5 3.141152067108976 7 6 6.650095346807543 7 7 3.6793701503834626 7 8 3.77937744447042 7 9 3.890723613099354 7 10 3.3366130775828253 7 11 3.8651201287603882 7 12 7.789630006169055 7 13 7.251785606280482 7 14 8.191732941851892 7 15 6.838703094619557 7 16 7.619951420627469 7 17 7.357337965490498 7 18 6.539958846818532 7 19 5.580418885121638 7 20 5.923066007732409 7 21 4.590868231929441 7 22 4.532202055326088 7 23 3.871894556033787 7 24 4.464219160520322 7 25 4.749989204988353 7 26 3.3018760278976202 7 27 3.489600874877034 7 28 2.4727937607455246 7 29 4.185408769100505 7 30 4.257454926081536 7 31 3.422919455543891 7 32 4.885485552442603 7 33 3.862065037843074 7 34 4.2222632388534365 7 35 3.7889083079337738 7 36 2.472330684357914 7 37 2.346262365679344 7 38 2.981208663813497 7 39 5.34369888427754 7 40 0.7876987999734659 7 41 -8.09370184763768 7 42 5.9044220511024825 7 43 9.078866725526453 7 44 8.900135468982718 7 45 5.807502809139933 7 46 9.632950769317173 7 47 8.706914125647952 7 48 10.162712415891292 7 49 11.723905876860456 7 50 9.015319819547923 7 51 10.748562939627494 7 52 9.799947779484139 7 53 10.03964202791284 7 54 10.269467190838302 7 55 10.44556843343193 7 56 10.867284351596911 8 1 2.2483546953048474 8 2 2.8569610797642397 8 3 4.227055549380054 8 4 2.857709464722028 8 5 2.942739368696273 8 6 6.477873124585319 8 7 3.4373066583199687 8 8 3.808742523835498 8 9 3.7923109146866523 8 10 3.845343236312985 8 11 3.884167747808007 8 12 7.261852228391277 8 13 7.434325288820165 8 14 8.117129767248716 8 15 7.387909443825908 8 16 7.149316499992548 8 17 6.993052251204777 8 18 6.636784243643927 8 19 5.881212535915289 8 20 5.66116124582765 8 21 3.6527729938341977 8 22 4.543313166437201 8 23 4.029037413176638 8 24 4.747552493853654 8 25 3.9991955541947064 8 26 3.068542694564286 8 27 3.5284897637659185 8 28 2.7870794750312413 8 29 3.5703294040211375 8 30 2.55904222766884 8 31 3.0387924714169046 8 32 3.6838982508553 8 33 2.860477736255767 8 34 2.835755302345504 8 35 1.8270035460290117 8 36 1.5588386208658491 8 37 1.5470560164729932 8 38 2.5558118384166733 8 39 3.310365550944205 8 40 -1.3488091365344737 8 41 -10.215130419066252 8 42 4.308390305070738 8 43 8.167755614415341 8 44 7.031087849935098 8 45 4.443217094854219 8 46 6.981363467729871 8 47 7.717231585965411 8 48 8.297633050811925 8 49 10.35009635305093 8 50 7.82166902589713 8 51 10.06602325708781 8 52 9.585662065198424 8 53 9.120594408865223 8 54 11.025022746393862 8 55 10.25112398898748 8 56 9.684744669057231 9 1 2.0674023143524636 9 2 2.15934203214519 9 3 4.054039676364184 9 4 2.610090417102981 9 5 2.3578187337756376 9 6 6.550095346807542 9 7 3.4460368170501283 9 8 3.21271077780375 9 9 3.0907236130993496 9 10 2.3866130775828225 9 11 4.631786795427054 9 12 6.506296672835724 9 13 6.451785606280485 9 14 7.608399608518564 9 15 5.905369761286227 9 16 6.6032847539608035 9 17 6.157337965490495 9 18 5.873292180151868 9 19 4.963752218454978 9 20 4.056399341065742 9 21 3.374201565262773 9 22 4.048868721992754 9 23 3.055227889367117 9 24 3.230885827186988 9 25 3.349989204988354 9 26 3.7352093612309503 9 27 2.9562675415437027 9 28 2.889460427412189 9 29 2.2854087691005063 9 30 2.690788259414866 9 31 2.5729194555438895 9 32 4.618818885775937 9 33 2.3120650378430696 9 34 2.988929905520102 9 35 2.7889083079337738 9 36 0.022330684357910968 9 37 0.4629290323460076 9 38 0.9645419971468279 9 39 1.7603655509442078 9 40 -1.1789678666932026 9 41 -4.477035180971015 9 42 5.704422051102483 9 43 7.94553339219312 9 44 7.166802135649384 9 45 4.724169475806601 9 46 8.116284102650503 9 47 8.340247458981285 9 48 7.562712415891291 9 49 10.40723921019379 9 50 7.598653152881255 9 51 7.38189627296083 9 52 7.966614446150807 9 53 7.906308694579511 9 54 7.469467190838305 9 55 6.928901766765261 9 56 6.983951018263582 10 1 2.1406263580683103 10 2 3.1973201742216943 10 3 3.6655150862002444 10 4 2.7827680127313954 10 5 2.963556438693672 10 6 6.672772942435959 10 7 3.1752717897277236 10 8 3.587300941738178 10 9 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114 42 4.415533162213595 114 43 -1.30446660780688 114 44 -0.044308975461728295 114 45 5.713058364695488 114 46 -2.0781603417939394 114 47 -11.854196985463158 114 48 -2.7817320285531544 114 49 -13.876094123139545 114 50 -1.9124579582298544 114 51 -0.3069926159280598 114 52 0.04994777948413898 114 53 -0.7325801943093779 114 54 -3.586088364717252 114 55 -0.6933204554569627 114 56 4.478395462708026 115 1 0.6278189810191321 115 2 1.0697586988118601 115 3 1.6977896763641809 115 4 0.5184237504363125 115 5 0.7765687337756404 115 6 3.502178680140876 115 7 1.1793701503834626 115 8 1.9064607778037512 115 9 1.2823902797660196 115 10 1.478279744249491 115 11 -1.9640465379062775 115 12 -7.814536660497609 115 13 -3.3732143937195183 115 14 -8.739517058148106 115 15 -2.8883802387137756 115 16 -10.8154652460392 115 17 -13.178078701176172 115 18 -3.7496244865148007 115 19 -3.446664448211692 115 20 -3.358183992267591 115 21 0.7658682319294385 115 22 0.994702055326087 115 23 0.5781445560337843 115 24 1.5600524938536537 115 25 1.0041558716550227 115 26 0.6414593612309503 115 27 1.2333508748770328 115 28 1.3019604274121903 115 29 1.7791587691005049 115 30 1.663704926081536 115 31 0.4187527888772209 115 32 -4.379097780890731 115 33 2.068315037843071 115 34 1.063929905520105 115 35 0.8805749746004423 115 36 3.4348306843579124 115 37 4.240012365679341 115 38 4.662458663813496 115 39 6.039532217610873 115 40 10.285615466640131 115 41 8.689631485695653 115 42 1.4502553844358168 115 43 3.25803339219312 115 44 1.3272188023160503 115 45 2.303336142473267 115 46 0.28295076931717134 115 47 -6.336835874352047 115 48 -3.6393709174420437 115 49 -4.344844123139545 115 50 0.6257364862145884 115 51 1.2693962729608295 115 52 0.612447779484139 115 53 0.4896420279128435 115 54 0.813217190838305 115 55 2.1122351000985944 115 56 4.231867684930247 116 1 0.5340689810191321 116 2 1.3617229845261463 116 3 1.7826111049356115 116 4 1.1148523218648805 116 5 1.2506758766327835 116 6 2.4334286801408744 116 7 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0.8896008748770328 117 28 1.139460427412189 117 29 0.43540876910050486 117 30 -0.5925450739184654 117 31 -3.5437472111227777 117 32 -8.647847780890732 117 33 -3.087934962156929 117 34 -2.7610700944798943 117 35 -2.594425025399559 117 36 -0.3776693156420876 117 37 0.19626236567934185 117 38 -2.918791336186505 117 39 0.2770322176108735 117 40 -0.44563453335986836 117 41 2.189631485695653 117 42 0.8877553844358168 117 43 -4.104466607806881 117 44 -1.0665311976839504 117 45 1.3408361424732682 117 46 -3.9670492306828287 117 47 -6.543085874352048 117 48 -7.670620917442044 117 49 -5.676094123139546 117 50 -5.468013513785412 117 51 -4.718103727039171 117 52 -4.3000522205158624 117 53 -4.060357972087157 117 54 -7.980532809161694 117 55 -4.987764899901407 117 56 -2.9493823150697516 118 1 0.9044393513895059 118 2 1.438971661774822 118 3 2.909595231919738 118 4 0.8471274541400149 118 5 1.3078187337756404 118 6 3.7371323838445782 118 7 1.7441849651982793 118 8 1.951599666692637 118 9 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2.500626160404856 119 30 1.648759273907622 119 31 -1.469834167644521 119 32 -11.43480430262986 119 33 -0.5770653969395383 119 34 -1.5175918336103322 119 35 -1.657468503660425 119 36 0.6658089452274751 119 37 1.0701754091575992 119 38 1.59859996816132 119 39 4.150945261089131 119 40 6.689148075335783 119 41 3.3418053987391296 119 42 -4.786157659042445 119 43 -2.0979448686764464 119 44 -5.233922502031776 119 45 -4.030902987961515 119 46 -8.586614448074133 119 47 -10.569172830873786 119 48 -12.105403526137696 119 49 -9.126094123139545 119 50 -9.935404818133238 119 51 -10.265929813995692 119 52 -12.004400046602818 119 53 -15.738618841652375 119 54 -12.400098026553 119 55 -15.029069247727492 119 56 -13.081991010721927 120 1 0.6720000155018937 120 2 1.269112147087725 120 3 3.1126603660193553 120 4 1.2468720262983837 120 5 1.1354049406721956 120 6 2.136876956002947 120 7 1.1017839434869074 120 8 0.4765038812520288 120 9 0.5022178659729164 120 10 1.0084521580425942 120 11 -8.436029296526968 120 12 -0.3856573501527798 120 13 -7.868904048891931 120 14 -5.777232575389487 120 15 -2.984285411127569 120 16 -11.941542832246096 120 17 -7.890363183934792 120 18 -4.817512417549285 120 19 -3.482224793039279 120 20 -5.266589164681385 120 21 -1.6608559060015935 120 22 -0.9815910481221906 120 23 -1.4832778577593189 120 24 -5.838654402698069 120 25 -1.1661027490346356 120 26 -0.19582512152766895 120 27 0.08787673694600073 120 28 -0.36398784844988086 120 29 0.34920187254878243 120 30 -0.190820935987432 120 31 -2.919609280088295 120 32 -12.761640884339009 120 33 -3.63103841043279 120 34 -11.836932163445411 120 35 -2.0892526116064545 120 36 -0.3431865570213972 120 37 -1.5520134963896233 120 38 -1.1705154741175399 120 39 0.0804804934729404 120 40 -6.124944878187456 120 41 -5.586230583269865 120 42 -9.92258944315039 120 43 -7.468259711255156 120 44 -9.878600163201192 120 45 -14.681577650630182 120 46 -15.889463023786277 120 47 -15.881016908834805 120 48 -15.08441402089032 120 49 -15.57436998520851 120 50 -14.752496272406102 120 51 -15.400862347728825 120 52 -15.182810841205516 120 53 -17.260357972087156 120 54 -16.323636257437556 120 55 -16.672247658522096 120 56 -14.989037487483547 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 450.0 5 78.0 6 66.0 7 51.0 8 71.0 9 51.0 10 49.0 11 56.0 12 51.0 13 78.0 14 115.0 15 154.0 16 224.0 17 330.0 18 369.0 19 450.0 20 598.0 21 682.0 22 899.0 23 1065.0 24 1118.0 25 1306.0 26 1294.0 27 1349.0 28 1433.0 29 1495.0 30 1684.0 31 1954.0 32 2294.0 33 2866.0 34 3313.0 35 4069.0 36 4731.0 37 4635.0 38 2807.0 39 757.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 100.0 0.0 0.0 7 99.51153703014515 0.41170450316337925 0.0581503535541496 0.018608113137327877 8 99.27893561592855 0.6187197618161518 0.06047636769631559 0.04186825455898772 9 97.67301158840229 1.5846791082980407 0.36999115744403593 0.37231814585563366 10 37.458131745441015 44.23148492742836 7.69212876814291 10.618254558987719 11 24.085876442128768 29.298474134722742 28.728600669892074 17.887048753256423 12 45.03628582061779 13.183848157796799 19.182638630442874 22.59722739114254 13 24.0905284704131 22.311127651656122 22.480926684034237 31.11741719389654 14 34.930200093066546 16.40995812005584 20.386226151698462 28.273615635179155 15 21.404775771694815 28.18171228887595 20.927198602213164 29.486313337216075 16 22.153194657731863 17.006375354832706 37.74954627949184 23.0908837079436 17 37.732644705006514 20.109808300763074 18.644146659222034 23.513400335008374 18 37.35985112816934 24.09630146545708 19.313793905559432 19.230053500814144 19 25.655461207397927 35.25648482028615 15.949749912760266 23.138304059555658 20 23.182875756165657 21.605397859469523 19.68590041879944 35.525825965565375 21 24.01888943168865 19.494265708237375 35.780584827971246 20.706260032102726 22 23.330464515828893 23.114140168872556 28.999092833383735 24.55630248191482 23 25.939709713435054 34.8948641607741 21.764514328247117 17.400911797543728 24 20.278191291403054 36.81847785634537 20.527074804614813 22.37625604763677 25 21.934823567723477 36.249447558791374 21.025330883208113 20.790397990277036 26 25.718879619704527 25.38332478911311 27.601715058023025 21.29608053315934 27 32.6151698464402 21.284318287575616 24.585853885528152 21.514657980456025 28 21.653616703952274 33.358967188665176 25.33090976882923 19.656506338553317 29 20.533221663874933 33.79396984924623 26.707612134747816 18.965196352131024 30 19.381107491856678 24.19730107026524 41.37505816658911 15.046533271288972 31 17.160977429584033 24.139619473978733 43.781477336317856 14.91792576011938 32 32.04913456169737 18.876791364228552 32.20268006700168 16.8713940070724 33 19.6797725312886 32.37933204372247 33.206702868995734 14.734192555993195 34 17.78895408400857 23.265344137096022 35.43121914873801 23.5144826301574 35 29.735831490748282 23.4027696962644 34.62818573257302 12.23321308041429 36 28.1916256961111 23.543118111703986 34.172938462613885 14.092317729571032 37 26.453508710557617 22.67543552788078 36.31381328855617 14.557242473005433 38 19.830879612374208 24.529444651509504 44.10408125232948 11.535594483786806 39 16.707542101698085 29.014551654871184 30.14504940088291 24.132856842547824 40 9.912249912249912 16.979056979056978 25.056745056745054 48.05194805194805 41 19.88551051051051 17.105386636636634 27.81766141141141 35.19144144144144 42 13.52869865249219 26.10155266470835 37.331561523756235 23.038187159043222 43 8.491028303427678 19.756399187997292 28.228760762535877 43.52381174603915 44 9.929674540314478 16.929510992733814 26.090511903927478 47.050302563024225 45 13.951646713560347 35.00722038477663 36.267293986118226 14.77383891554479 46 25.447478062518915 24.446616856364777 36.74557177105881 13.36033331005749 47 14.863072663393751 22.003769282672934 43.81674771399986 19.31641033993346 48 15.714518418541875 25.36941800665534 45.91254974053475 13.003513834268029 49 10.438048143310318 17.2490203396156 26.380854637059155 45.932076880014925 50 12.416913496746508 25.008745947710892 49.57902838351564 12.99531217202696 51 26.748855888670963 25.05603810591202 36.55552442327449 11.639581582142524 52 14.944649446494465 36.1600261572236 35.76066140408239 13.13466299219954 53 24.7126302781599 27.564177294877474 34.8271118468605 12.896080580102124 54 13.14888562844966 24.057383730408255 40.739188150632295 22.054542490509792 55 16.14113589223706 23.75492320958307 36.71956932112144 23.384371577058428 56 26.84473034074488 24.250687549526873 33.31235724607281 15.592224863655431 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 1.0 5 2.0 6 1.0 7 0.0 8 0.0 9 0.0 10 23.0 11 46.0 12 74.5 13 126.0 14 149.0 15 155.0 16 161.0 17 219.0 18 277.0 19 325.0 20 373.0 21 428.5 22 603.0 23 722.0 24 928.5 25 1135.0 26 1298.0 27 1461.0 28 1689.0 29 2001.5 30 2086.0 31 2004.5 32 1923.0 33 1745.5 34 1568.0 35 1420.5 36 1273.0 37 1136.5 38 899.5 39 799.0 40 808.5 41 818.0 42 832.5 43 847.0 44 957.0 45 1067.0 46 1481.0 47 2229.0 48 2563.0 49 2532.0 50 2501.0 51 2289.5 52 2078.0 53 2073.0 54 2045.0 55 2022.0 56 1956.5 57 1891.0 58 1890.0 59 1889.0 60 1829.5 61 1770.0 62 1669.5 63 1491.0 64 1413.0 65 1208.0 66 1003.0 67 875.5 68 748.0 69 611.0 70 474.0 71 412.0 72 301.5 73 253.0 74 212.0 75 171.0 76 124.0 77 77.0 78 52.5 79 20.5 80 13.0 81 9.0 82 5.0 83 2.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.04186825455898772 10 0.0 11 0.0 12 0.0 13 0.0 14 0.02791216970599181 15 0.15584294752512096 16 0.032564197990323784 17 0.018608113137327877 18 0.004652028284331969 19 0.016282098995161892 20 0.02791216970599181 21 0.011630070710829922 22 0.0023260141421659847 23 0.0 24 0.0 25 0.0023260141421659847 26 0.18142910308894677 27 0.02791216970599181 28 0.18608113137327875 29 0.018608113137327877 30 0.02791216970599181 31 0.24190547078526237 32 0.018608113137327877 33 0.19771120208410867 34 0.10234462225530332 35 0.06280238183848158 36 0.1744510606624488 37 0.2628395980647562 38 0.14886490509862302 39 0.41635653144771123 40 0.597785634536658 41 0.8559732043170823 42 0.22794938593226646 43 0.3140119091924079 44 0.444268701153703 45 0.1349088202456271 46 0.06745441012281354 47 0.030238183848157796 48 0.044194268701153706 49 0.27912169705991813 50 0.26749162634908824 51 0.38146631931522146 52 0.40472646073688123 53 0.23957945664309638 54 0.12327874953479717 55 0.1930591737997767 56 0.20003721622627466 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 42992.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.04354298474135 #Duplication Level Percentage of deduplicated Percentage of total 1 93.95272888298462 78.96120208410868 2 3.954942986826082 6.647748418310383 3 0.8773386471825529 2.2120394491998514 4 0.41237684047381823 1.3863044287309267 5 0.21033986493966564 0.883885374023074 6 0.09686704306431972 0.48846296985485665 7 0.10516993246983282 0.6187197618161518 8 0.06365548544226723 0.4279866021585412 9 0.04981733643307871 0.3768142910308895 >10 0.23524853315620506 3.6402121324897654 >50 0.019373408612863945 1.2537216226274657 >100 0.02214103841470165 3.102902865649423 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGATCAGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG 218 0.5070710829921845 No Hit TGATCAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG 203 0.47218087085969485 No Hit TGATCAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG 183 0.4256605880163751 No Hit TGATCAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG 180 0.41868254558987716 No Hit TGATCAGGGATGAGATGGACTTAAATGAATTGACGGGTACCCACAGTTCTGAGTCG 176 0.40937848902121327 No Hit TGATCAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG 159 0.3698362486043915 No Hit TGATCAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC 114 0.2651656122069222 No Hit TGATCAGGGATGAGATGGACTCAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG 101 0.23492742835876443 No Hit TGATCAGGGATGAGATGGACTCAAATGAATTGACGGGTACCCACAGTTCTGAGTCG 93 0.21631931522143655 No Hit TGATCAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG 84 0.1953851879419427 No Hit TGATCAGGGATGAGATGGACTCAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG 78 0.18142910308894677 No Hit TGATCAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG 78 0.18142910308894677 No Hit TGATCAGGGATGCGCTGGACTTAAATGAATTGACGGGTCCGCCCAGTTCTGAGTCG 71 0.1651470040937849 No Hit TGATCAGGGATGCGCTGGACTTAAAGGAATTGACGGGTCCGCCCAGTTCTGAGTCG 69 0.16049497580945293 No Hit TGATCAGGGATGAGATGGACTCAAATGAATTGACGGGTACGCACAGTTCTGAGTCG 66 0.15351693338295497 No Hit TGATCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC 48 0.11164867882396724 No Hit TGATCAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCGAACCTGCGTGCAATC 45 0.10467063639746929 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0046520282843319685 0.0 0.0 0.0 0.0 7 0.006978042426497953 0.0 0.0 0.0 0.0 8 0.006978042426497953 0.0 0.0 0.0 0.0 9 0.006978042426497953 0.0 0.0 0.0 0.0 10 0.009304056568663937 0.0 0.0 0.0 0.0 11 0.013956084852995905 0.0 0.0 0.0 0.0 12 0.013956084852995905 0.0 0.0 0.0 0.0 13 0.016282098995161892 0.0 0.0 0.0 0.0 14 0.023260141421659844 0.0 0.0 0.0 0.0 15 0.023260141421659844 0.0 0.0 0.0 0.0 16 0.023260141421659844 0.0 0.0 0.0 0.0 17 0.030238183848157796 0.0 0.0 0.0 0.0 18 0.03721622627465575 0.0 0.0 0.0 0.0 19 0.03721622627465575 0.0 0.0 0.0 0.0 20 0.039542240416821736 0.0 0.0 0.0 0.0 21 0.04186825455898772 0.0 0.0 0.0 0.0 22 0.04652028284331969 0.0 0.0 0.0 0.0 23 0.06047636769631559 0.0 0.0 0.0 0.0 24 0.07210643840714552 0.0 0.0 0.0 0.0 25 0.07675846669147748 0.0 0.0 0.0 0.0 26 0.08606252326014142 0.0 0.0 0.0 0.0 27 0.09536657982880536 0.0 0.0 0.0 0.0 28 0.10699665053963528 0.0 0.0 0.0 0.0 29 0.10699665053963528 0.0 0.0 0.0 0.0 30 0.10699665053963528 0.0 0.0 0.0 0.0 31 0.10932266468180127 0.0 0.0 0.0 0.0 32 0.10932266468180127 0.0 0.0 0.0 0.0 33 0.10932266468180127 0.0 0.0 0.0 0.0 34 0.10932266468180127 0.0 0.0 0.0 0.0 35 0.10932266468180127 0.0 0.0 0.0 0.0 36 0.10932266468180127 0.0 0.0 0.0 0.0 37 0.10932266468180127 0.0 0.0 0.0 0.0 38 0.10932266468180127 0.0 0.0 0.0 0.0 39 0.10932266468180127 0.0 0.0 0.0 0.0 40 0.10932266468180127 0.0 0.0 0.0 0.0 41 0.11630070710829922 0.0 0.0 0.0 0.0 42 0.11630070710829922 0.0 0.0 0.0 0.0 43 0.11862672125046521 0.0 0.0 0.0 0.0 44 0.12327874953479717 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTAAAA 20 3.884566E-4 50.644127 39 ACCTGTG 15 0.007961928 50.644127 40 ACACAGT 15 0.007961928 50.644127 41 ACTTCCA 15 0.007961928 50.644127 39 AACTAAA 20 3.884566E-4 50.644127 38 AGACCCT 15 0.007961928 50.644127 40 GACCTGT 15 0.007961928 50.644127 39 TTAATTA 15 0.007961928 50.644127 41 TACTCAC 15 0.007961928 50.644127 38 CCGACCA 15 0.007961928 50.644127 39 GAGACCC 15 0.007961928 50.644127 39 AAACTAA 20 3.9074113E-4 50.584126 37 ATACGCC 20 3.9074113E-4 50.584126 37 GTCCGAC 15 0.007999393 50.58412 37 GTACACA 15 0.007999393 50.58412 37 CGGCCAA 25 1.9168287E-5 50.58412 37 GGTCCGA 15 0.008112576 50.40496 36 GGTACTC 15 0.008112576 50.40496 36 GATACGC 25 1.9576091E-5 50.404957 36 GGATACG 25 1.9713612E-5 50.34552 35 >>END_MODULE