FastQCFastQC Report
Fri 17 Jun 2016
SRR1526307_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526307_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29844
Sequences flagged as poor quality0
Sequence length56
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTCCGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC600.2010454362685967No Hit
GCGTCCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG540.18094089264173704No Hit
GCGTCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC410.13738104811687443No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAACCT150.00803055150.4957439
TGGTGGC150.00819442150.23898334
CCAGCCC150.00824959450.15397643
CCCACAC150.00824959450.15397643
CTTCTGT252.0418747E-549.98482528
AGAAGCA150.00836076849.9848250
GCAACAT301.0155509E-649.9848223
CTGCAAT301.0155509E-649.9848249
GCCTGCA301.0155509E-649.9848247
CCGTGGT150.00841677149.90067313
CCAGTGA150.00841677149.90067313
CGTGGTA150.00841677149.90067314
TCTCGCT150.00841677149.90067320
AGTGACA150.00841677149.90067315
GAGGCCC150.00847305349.8168079
ATCCAGT150.00847305349.81680711
GAAGCAT301.0395288E-649.8168079
CTCTCGC150.00847305349.81680719
GTGGGCG150.00847305349.81680710
GATCCAG150.00847305349.81680710
Produced by FastQC (version 0.11.5)