##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526307_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 29844 Sequences flagged as poor quality 0 Sequence length 56 %GC 55 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.97962739579145 39.0 38.0 39.0 35.0 40.0 2 37.107659831121836 39.0 38.0 39.0 33.0 40.0 3 37.548787025867846 39.0 38.0 39.0 35.0 40.0 4 37.44762766385203 39.0 38.0 39.0 35.0 40.0 5 37.54761426082295 39.0 38.0 39.0 35.0 40.0 6 37.55300898002949 38.0 38.0 39.0 35.0 40.0 7 36.684861278648974 38.0 36.0 39.0 33.0 40.0 8 36.73314569092615 38.0 36.0 39.0 33.0 40.0 9 36.78092748961265 38.0 36.0 39.0 33.0 40.0 10 36.8739780190323 38.0 36.0 39.0 33.0 40.0 11 35.40993164455167 38.0 35.0 39.0 29.0 40.0 12 33.248056560782736 37.0 31.0 39.0 20.0 40.0 13 32.641401956842245 36.0 30.0 39.0 17.0 40.0 14 32.292889693070634 36.0 29.0 39.0 16.0 40.0 15 32.88577268462672 37.0 30.0 39.0 18.0 40.0 16 32.601125854443104 36.0 30.0 38.0 19.0 40.0 17 32.53655676182817 36.0 30.0 38.0 19.0 40.0 18 32.62488272349551 36.0 30.0 38.0 19.0 40.0 19 33.46934057096904 36.0 31.0 38.0 24.0 40.0 20 33.66351695483179 36.0 31.0 38.0 24.0 40.0 21 34.95885270071036 38.0 34.0 39.0 27.0 40.0 22 34.618181208953224 38.0 33.0 39.0 26.0 40.0 23 34.83665058303176 38.0 33.0 39.0 27.0 40.0 24 34.44059107358263 38.0 33.0 39.0 25.0 40.0 25 34.680505294196486 38.0 33.0 39.0 26.0 40.0 26 34.99437072778448 38.0 35.0 39.0 27.0 40.0 27 34.91951481034714 38.0 35.0 39.0 27.0 40.0 28 34.8229794933655 38.0 35.0 39.0 27.0 40.0 29 34.6632488942501 38.0 34.0 39.0 27.0 40.0 30 34.52251708886208 38.0 34.0 39.0 26.0 40.0 31 34.24611312156547 38.0 33.0 39.0 25.0 40.0 32 33.08350087119689 37.0 32.0 39.0 18.0 40.0 33 34.383594692400486 38.0 34.0 39.0 26.0 40.0 34 34.09620024125452 38.0 33.0 39.0 24.0 40.0 35 34.00207746950811 38.0 33.0 39.0 24.0 40.0 36 32.007572711432786 35.0 31.0 38.0 20.0 39.0 37 31.71702854845195 35.0 31.0 38.0 18.0 39.0 38 30.95161506500469 35.0 30.0 38.0 11.0 39.0 39 29.005495241924674 33.0 26.0 38.0 4.0 39.0 40 24.892306661305454 30.0 11.0 37.0 4.0 39.0 41 21.65755260688916 25.0 9.0 33.0 4.0 37.0 42 25.290209087253718 30.0 19.0 34.0 4.0 37.0 43 21.296240450341777 25.0 7.0 33.0 4.0 36.0 44 22.420989143546443 27.0 9.0 33.0 4.0 36.0 45 24.9152928561855 30.0 18.0 34.0 4.0 37.0 46 24.945918777643747 30.0 12.0 36.0 4.0 38.0 47 24.133192601527945 29.0 7.0 35.0 4.0 38.0 48 24.049691730331055 29.0 8.0 35.0 4.0 38.0 49 21.544062458115533 26.0 4.0 35.0 4.0 38.0 50 23.81084975204396 29.0 4.0 35.0 4.0 38.0 51 25.26986999061788 32.0 4.0 38.0 4.0 39.0 52 24.999798954563733 31.0 4.0 37.0 4.0 39.0 53 24.58701916633159 31.0 4.0 37.0 4.0 39.0 54 23.75395389357995 30.0 4.0 37.0 4.0 39.0 55 23.903699236027343 31.0 4.0 36.0 4.0 39.0 56 23.124782200777375 30.0 4.0 37.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.17726753651123062 1 2 0.9210816785972895 1 3 0.12003775361798574 1 4 0.07085788137225535 1 5 0.379759271712409 1 6 0.17731285228123994 1 7 1.460973751403472 1 8 1.4781829737745298 1 9 1.5725292406199927 1 10 -0.2201353427474757 1 11 -4.534300480935158 1 12 -9.669952035488521 1 13 -21.030873080379745 1 14 -19.50316424665147 1 15 -11.603066514224814 1 16 -22.57838411861602 1 17 -22.646301207903726 1 18 -21.32921113321792 1 19 -7.044990489662496 1 20 -12.491187446909116 1 21 -9.656415078310964 1 22 -3.9005277256823803 1 23 -4.881535671674193 1 24 -4.27815350250188 1 25 -5.039492918341175 1 26 1.3395909165097208 1 27 0.9108425020755746 1 28 0.9870429752443144 1 29 0.5556050999894282 1 30 1.3021000220078847 1 31 0.5285591252278294 1 32 -12.916494612997667 1 33 -0.013843334458734091 1 34 -0.2323295627177373 1 35 -0.06468958182510676 1 36 0.37971378652611776 1 37 1.3090044048939937 1 38 1.6995931936534703 1 39 3.9836773472809206 1 40 6.812517874251082 1 41 -2.6740626556109426 1 42 -10.166625806688174 1 43 -12.691914850345444 1 44 -16.083386553142855 1 45 -15.842920602888967 1 46 -18.54672018636493 1 47 -17.955303680418112 1 48 -16.5482182165038 1 49 -14.59189105280237 1 50 -17.307806781105334 1 51 -18.18636447912834 1 52 -17.879427554710155 1 53 -17.480936381566877 1 54 -17.372262255835167 1 55 -16.89393453555016 1 56 -16.1171504167361 2 1 0.3867205587268643 2 2 1.163641202406815 2 3 0.07688299171322655 2 4 0.33573883375320435 2 5 0.4824378431409784 2 6 0.22046761418599914 2 7 1.2288308942606179 2 8 1.6091353547269094 2 9 1.6186601930009417 2 10 0.6399837048715753 2 11 -2.4241814333161074 2 12 -8.970547273583758 2 13 -10.193075461332125 2 14 -13.611795199032422 2 15 -9.37687603803434 2 16 -4.064991261473164 2 17 -2.9900512079037256 2 18 -9.603020657027447 2 19 -3.098561918233923 2 20 -3.6414850659567364 2 21 1.9358468264509412 2 22 1.9908413219366707 2 23 1.9458452807067559 2 24 2.1846441165457406 2 25 1.2968166054683508 2 26 2.5003052022240055 2 27 2.452509168742239 2 28 1.2176977371490736 2 29 2.466319385703713 2 30 1.937516688674549 2 31 -2.3241194462007435 2 32 -8.470066041569094 2 33 -2.085271905887307 2 34 -1.7338176579558322 2 35 -1.436713391348917 2 36 4.073166167478497 2 37 3.6453139287035192 2 38 4.3989979555582295 2 39 6.36165353775711 2 40 8.781267874251082 2 41 5.5670087729604845 2 42 1.595278955216589 2 43 -3.6487600884406817 2 44 -9.514934172190474 2 45 0.8296984447300808 2 46 -9.823505900650643 2 47 -9.650244156608588 2 48 -6.881551549837132 2 49 -11.96093867184999 2 50 -6.225961543010097 2 51 -5.9185073362711975 2 52 -5.516332316614918 2 53 -6.74581733394783 2 54 -14.226428922501835 2 55 -7.34036310697873 2 56 -6.832924226259909 3 1 0.49556409614183394 3 2 1.5581990255360623 3 3 0.5326653046383925 3 4 0.8221333915763367 3 5 1.2579480472226123 3 6 1.2034608114649146 3 7 1.943116608546326 3 8 2.22818297377453 3 9 2.3703608732730572 3 10 1.1637932286810937 3 11 0.8955464578403536 3 12 0.36958878083800784 3 13 -5.965184304869542 3 14 -7.057373430324937 3 15 -3.4551073305513462 3 16 2.4656209834247917 3 17 2.80586715944321 3 18 2.288135805557591 3 19 1.7891931838068942 3 20 1.2326645939072094 3 21 2.80659512577067 3 22 3.7697528865625145 3 23 3.258770450774783 3 24 2.2798822117838355 3 25 2.783211163291476 3 26 3.0411215287546156 3 27 1.908291481667412 3 28 2.649670526264721 3 29 2.833666324479225 3 30 1.760645940375234 3 31 2.910574431350277 3 32 1.8360564074104957 3 33 3.4045240124800387 3 34 2.7797877842210355 3 35 3.056483887562649 3 36 5.579968888566931 3 37 5.927626853873587 3 38 5.922807479367755 3 39 8.385463061566636 3 40 11.44453318037353 3 41 8.20986591581763 3 42 6.190517050454684 3 43 0.9158657618994539 3 44 2.1347256917551043 3 45 4.680038580784501 3 46 0.829555323839152 3 47 -3.357727149805868 3 48 -0.027810053238493282 3 49 -9.66842166504727 3 50 -1.2905873933502328 3 51 -2.8980991730058925 3 52 -1.4687132689958702 3 53 -1.6607833203423894 3 54 -4.192415317059655 3 55 -2.5444447396317926 3 56 1.241905705712881 4 1 1.0714824634887705 4 2 1.9198316785972906 4 3 1.3187877536179826 4 4 1.263357881372258 4 5 1.4910092717124073 4 6 1.478562852281243 4 7 1.7259737514034725 4 8 2.4281829737745326 4 9 2.6662792406199927 4 10 1.5523646572525251 4 11 2.668199519064842 4 12 1.967547964511482 4 13 4.027876919620258 4 14 2.820585753348528 4 15 4.7669334857751835 4 16 6.137865881383981 4 17 5.429948792096276 4 18 5.70078886678208 4 19 4.510009510337504 4 20 4.217562553090886 4 21 2.491084921689037 4 22 3.26322227431762 4 23 2.3934643283258055 4 24 3.4255964974981197 4 25 2.6330070816588247 4 26 2.6545909165097186 4 27 1.6658425020755772 4 28 1.7157929752443124 4 29 2.340605099989425 4 30 1.590850022007885 4 31 2.38730912522783 4 32 3.3185053870023324 4 33 1.4461566655412668 4 34 3.0814204372822616 4 35 2.85281041817489 4 36 5.052213786526117 4 37 4.861504404893996 4 38 5.177093193653469 4 39 7.611177347280918 4 40 10.161267874251084 4 41 3.8584373443890563 4 42 7.113374193311827 4 43 6.609335149654555 4 44 9.664113446857144 4 45 8.322079397111033 4 46 10.190779813635068 4 47 10.38594631958189 4 48 9.251781783496202 4 49 5.79810894719763 4 50 8.915943218894665 4 51 7.418635520871661 4 52 8.025572445289846 4 53 5.766563618433121 4 54 0.9802377441648318 4 55 5.45106546444984 4 56 5.176599583263901 5 1 0.5680782081696236 5 2 0.8313210402994144 5 3 -1.0548292676586115 5 4 -0.5421740335213627 5 5 0.42164756958474925 5 6 0.5330309373876219 5 7 0.8093780067226177 5 8 1.887757441859634 5 9 1.5492579640242496 5 10 -0.7055076831730105 5 11 2.1452207956605847 5 12 4.693079879405097 5 13 5.453834366428769 5 14 4.989521923561295 5 15 5.966082421945401 5 16 5.099142477128659 5 17 4.797182834649469 5 18 5.388873973165058 5 19 2.9576690848055875 5 20 3.6477753190483284 5 21 2.5842764110507375 5 22 1.9325839764452795 5 23 1.5041026261981472 5 24 2.6081496889874813 5 25 1.1240709114460614 5 26 1.3316121931054639 5 27 1.7432893105862135 5 28 -0.6186751098620675 5 29 1.1806050999894282 5 30 0.7176585326461833 5 31 0.40688359331293356 5 32 1.4904202806193538 5 33 -0.5989497174374563 5 34 0.8188672457929016 5 35 0.5111082905153168 5 36 1.064554212058031 5 37 0.2810788729791014 5 38 2.4668804276960223 5 39 3.181815645153261 5 40 3.2599912785064014 5 41 -0.14879669816413355 5 42 5.333374193311826 5 43 10.738271319867323 5 44 9.433900680899697 5 45 6.462930460940822 5 46 7.678013856188262 5 47 8.102542064262739 5 48 7.739015826049393 5 49 8.317683415282737 5 50 7.333815559320197 5 51 7.023741903850382 5 52 8.054083083587717 5 53 8.429967873752272 5 54 8.014705829271215 5 55 9.018299507003032 5 56 9.529791072625603 6 1 1.0164824634887708 6 2 2.614831678597291 6 3 1.388787753617983 6 4 0.6333578813722553 6 5 2.0610092717124076 6 6 1.8585628522812385 6 7 2.6859737514034734 6 8 2.6781829737745326 6 9 2.5662792406199912 6 10 1.8173646572525257 6 11 3.6531995190648416 6 12 6.542547964511478 6 13 6.462876919620253 6 14 5.5655857533485324 6 15 6.546933485775185 6 16 6.3778658813839755 6 17 6.359948792096276 6 18 5.8207888667820775 6 19 4.255009510337501 6 20 4.927562553090887 6 21 3.481084921689039 6 22 3.668222274317621 6 23 2.8434643283258083 6 24 3.715596497498119 6 25 2.5730070816588224 6 26 3.0395909165097237 6 27 1.8358425020755718 6 28 0.8807929752443115 6 29 2.7806050999894296 6 30 2.1708500220078832 6 31 2.1723091252278266 6 32 2.4835053870023316 6 33 1.1361566655412645 6 34 2.8864204372822613 6 35 2.8728104181748932 6 36 2.167213786526119 6 37 1.7465044048939937 6 38 3.7370931936534717 6 39 4.946177347280919 6 40 1.5812678742510826 6 41 2.3884373443890574 6 42 5.4083741933118255 6 43 12.064335149654557 6 44 10.404113446857146 6 45 6.057079397111032 6 46 8.34077981363507 6 47 9.200946319581888 6 48 8.751781783496202 6 49 10.63310894719763 6 50 8.935943218894664 6 51 8.38863552087166 6 52 9.120572445289845 6 53 8.881563618433123 6 54 9.465237744164831 6 55 7.656065464449842 6 56 9.851599583263901 7 1 1.0124126960469084 7 2 1.6863433065042628 7 3 1.6759970559435686 7 4 1.0461485790466725 7 5 1.631939504270548 7 6 1.679493084839379 7 7 2.109229565356962 7 8 1.972369020286159 7 9 2.096511798759529 7 10 1.3132948898106633 7 11 3.6212227748787953 7 12 5.104757266837062 7 13 5.478574594038857 7 14 6.6312834277671335 7 15 6.577166043914723 7 16 6.263912393011886 7 17 6.568088326979996 7 18 5.757998169107662 7 19 5.4346606731281994 7 20 4.105469529835069 7 21 3.3799221309913605 7 22 3.4432222743176197 7 23 1.7609061887909263 7 24 2.54234068354463 7 25 1.9241698723565008 7 26 2.0634281258120453 7 27 1.0184006416104552 7 28 2.188351114779195 7 29 2.296884169756872 7 30 1.7127104871241627 7 31 2.8868440089487564 7 32 2.935249573048843 7 33 2.0210403864714976 7 34 2.4195599721659846 7 35 1.1983918135237275 7 36 0.7270975074563495 7 37 0.4122020793125998 7 38 1.2650001703976557 7 39 2.9967587426297584 7 40 -1.1373367769117095 7 41 -10.873190562587686 7 42 3.7054672165676408 7 43 8.68235840546851 7 44 7.604113446857145 7 45 5.037893350599404 7 46 7.914035627588557 7 47 7.686411435860958 7 48 8.044805039310155 7 49 9.309271737895305 7 50 7.5243153119179205 7 51 8.79910063715073 7 52 7.710688724359613 7 53 9.567028734712192 7 54 10.486749372071806 7 55 9.161298022589378 7 56 10.162646094891809 8 1 0.9219172460974647 8 2 2.161570809032071 8 3 1.5344399275310323 8 4 0.861618750937474 8 5 1.4979657934515416 8 6 1.6759541566290679 8 7 2.2381476644469487 8 8 2.749922104209311 8 9 2.9488879362721647 8 10 1.7662777007307824 8 11 3.093416910369193 8 12 5.724069703641916 8 13 6.209616050055036 8 14 6.07971618813114 8 15 6.820846529253444 8 16 7.230039794427455 8 17 6.596905313835407 8 18 6.6164410406951255 8 19 5.3952269016418555 8 20 5.107997335699579 8 21 2.9876066608194733 8 22 4.20409183953501 8 23 1.8630295457171115 8 24 3.4569008453242063 8 25 2.9469201251370833 8 26 3.062417003466244 8 27 3.6119294585973165 8 28 2.890575583939963 8 29 3.9849529260763816 8 30 2.509980456790494 8 31 4.160352603488697 8 32 4.936766256567552 8 33 4.007895795976047 8 34 3.2309856546735674 8 35 4.090201722522721 8 36 0.49656161261307474 8 37 0.6867217961983414 8 38 0.8544844980012947 8 39 3.8450903907591822 8 40 -4.370906038792395 8 41 -9.513736568654421 8 42 3.746417671572697 8 43 9.937161236611079 8 44 8.082374316422364 8 45 5.695122875371904 8 46 9.412518944069852 8 47 10.021598493494935 8 48 10.38656439219185 8 49 12.201587208067195 8 50 10.883769305851189 8 51 12.851678999132528 8 52 13.158615923550713 8 53 11.576128835824427 8 54 13.3532812224257 8 55 11.013674160102013 8 56 10.45486045282912 9 1 1.316482463488768 9 2 2.164831678597288 9 3 1.4887877536179843 9 4 0.9083578813722539 9 5 1.911009271712409 9 6 2.008562852281237 9 7 2.3609737514034705 9 8 2.60318297377453 9 9 2.9412792406199912 9 10 1.4673646572525243 9 11 3.253199519064843 9 12 6.1175479645114805 9 13 6.162876919620256 9 14 6.140585753348528 9 15 6.471933485775189 9 16 6.977865881383977 9 17 6.159948792096273 9 18 6.620788866782082 9 19 5.905009510337507 9 20 4.627562553090883 9 21 3.4560849216890333 9 22 3.918222274317621 9 23 2.5934643283258083 9 24 4.290596497498122 9 25 3.3980070816588253 9 26 2.7395909165097194 9 27 2.3608425020755774 9 28 2.9557929752443144 9 29 3.5806050999894268 9 30 3.2958500220078832 9 31 1.9473091252278323 9 32 4.183505387002334 9 33 2.5611566655412688 9 34 2.6114204372822627 9 35 2.1728104181748904 9 36 1.3172137865261178 9 37 2.7465044048939937 9 38 2.962093193653473 9 39 4.171177347280921 9 40 -0.8937321257489188 9 41 -2.2365626556109426 9 42 5.808374193311828 9 43 9.739335149654554 9 44 8.654113446857146 9 45 5.382079397111035 9 46 9.740779813635072 9 47 8.600946319581887 9 48 9.276781783496201 9 49 12.058108947197628 9 50 8.510943218894667 9 51 8.48863552087166 9 52 9.295572445289842 9 53 8.281563618433122 9 54 9.265237744164832 9 55 9.031065464449842 9 56 8.201599583263903 10 1 0.40511882712513625 10 2 0.8671044058700161 10 3 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36 6.152213786526119 114 37 5.941504404893994 114 38 7.117093193653467 114 39 9.35117734728092 114 40 13.081267874251079 114 41 12.118437344389054 114 42 6.733374193311825 114 43 -2.1306648503454433 114 44 0.20411344685714639 114 45 5.702079397111035 114 46 -0.7092201863649308 114 47 -9.654053680418112 114 48 -2.2882182165037968 114 49 -12.90189105280237 114 50 -1.6840567811053333 114 51 0.6186355208716598 114 52 0.4455724452898444 114 53 0.5065636184331233 114 54 -1.0397622558351678 114 55 0.9510654644498402 114 56 5.2565995832639025 115 1 -0.25397208196577736 115 2 1.048922587688196 115 3 0.06606048089071237 115 4 0.019721517735888483 115 5 0.09282745353058885 115 6 -0.1778007840823932 115 7 0.4041555695852921 115 8 1.0918193374108967 115 9 1.5299156042563595 115 10 -0.862180797292929 115 11 -1.4263459354806116 115 12 -3.9597247627612475 115 13 -1.448486716743382 115 14 -9.432141519378742 115 15 -2.1257937869520873 115 16 -11.603952300434205 115 17 -10.308233026085542 115 18 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2.1556117121719893 116 39 0.9674736435772182 116 40 3.8738604668436736 116 41 1.5273262332779467 116 42 -3.4444035844659524 116 43 -10.632887072567666 116 44 -3.7662569235132253 116 45 -2.8475502325185964 116 46 -9.627738704883448 116 47 -11.32220182856626 116 48 -11.52969969798528 116 49 -8.971520682432 116 50 -6.4825752996238535 116 51 -4.987290405054267 116 52 -5.8285016287842275 116 53 -6.700843788974284 116 54 -7.004206700279612 116 55 -7.62078638740201 116 56 -2.949326342662026 117 1 0.4914824634887722 117 2 1.3731650119306238 117 3 0.9054544202846557 117 4 0.7500245480389225 117 5 0.31100927171240755 117 6 0.29189618561457564 117 7 1.152640418070142 117 8 0.9615163071078641 117 9 1.766279240619994 117 10 0.92569799058586 117 11 -7.138467147601826 117 12 -10.065785368821853 117 13 -13.84545641371308 117 14 -11.226080913318135 117 15 -7.61973318089148 117 16 -11.455467451949357 117 17 -7.323384541237058 117 18 -4.012544466551255 117 19 -3.6366571563291608 117 20 -4.055770780242451 117 21 1.6977515883557004 117 22 1.8432222743176183 117 23 1.926797661659137 117 24 -1.7010701691685455 117 25 1.8730070816588267 117 26 3.647924249843051 117 27 2.7191758354089046 117 28 3.422459641910983 117 29 3.9806050999894254 117 30 3.4708500220078875 117 31 2.4139757918944937 117 32 -12.574827946331002 117 33 1.486156665541266 117 34 2.66142043728226 117 35 2.7394770848415604 117 36 2.992213786526115 117 37 4.554837738227324 117 38 5.503759860320137 117 39 6.271177347280922 117 40 2.481267874251081 117 41 5.5051040110557246 117 42 4.5333741933118255 117 43 -7.610664850345444 117 44 -7.6625532198095225 117 45 2.5154127304443676 117 46 -2.175886853031596 117 47 -10.44072034708478 117 48 -4.448218216503797 117 49 -10.54189105280237 117 50 -0.5640567811053359 117 51 0.071968854204993 117 52 1.5789057786231773 117 53 -0.8934363815668789 117 54 -7.026428922501834 117 55 -3.835601202216825 117 56 2.3099329165972335 118 1 -0.5266993546930507 118 2 0.8216498604154694 118 3 0.020605935436165623 118 4 -0.0711875731731979 118 5 0.09282745353058885 118 6 -0.04143714771876006 118 7 0.8587010241307453 118 8 0.8645466101381629 118 9 1.0753701497108992 118 10 0.13781920270707104 118 11 -4.517255026389705 118 12 -9.368815671852158 118 13 -7.357577625834292 118 14 -2.2048687921060157 118 15 -17.125793786952087 118 16 -7.467588664070568 118 17 -6.671869389721909 118 18 -4.579211133217921 118 19 -3.7608995805715857 118 20 -4.886073810545479 118 21 1.0765394671435828 118 22 1.6250404561357996 118 23 1.12982796468944 118 24 -3.5253125934109697 118 25 1.9093707180224584 118 26 2.0782272801460877 118 27 2.4222061384392077 118 28 2.2739747934261345 118 29 2.0896960090803347 118 30 2.679940931098791 118 31 1.2018545797732827 118 32 -6.677858249361304 118 33 0.9861566655412659 118 34 2.0886931645549893 118 35 1.73644678181126 118 36 3.6013046956170243 118 37 3.8033225867121736 118 38 5.95527501183529 118 39 6.080268256371827 118 40 7.0085406015238085 118 41 5.456619162570874 118 42 2.6515560114936427 118 43 -2.956119395799991 118 44 -2.5777047349610385 118 45 -1.5361024210707832 118 46 -6.018311095455839 118 47 -8.264962771327204 118 48 -8.820945489231072 118 49 -7.160072870984189 118 50 -5.645874962923518 118 51 -7.879546297310158 118 52 -8.481700281982882 118 53 -10.702527290657786 118 54 -10.92339861947153 118 55 -10.541661808277432 118 56 -9.11430950764519 119 1 -1.024307010195443 119 2 0.5872000996499196 119 3 -0.05594908848727442 119 4 0.14651577610909783 119 5 0.5425882190808267 119 6 -0.04143714771876006 119 7 0.8754474356139994 119 8 1.7544987632482147 119 9 1.7978581879884104 119 10 -0.8813195532737907 119 11 -7.892853112514107 119 12 -8.469294140751678 119 13 -2.0489651856429028 119 14 -1.4752037203356778 119 15 -1.5109612510669201 119 16 -9.501081487037073 119 17 -5.726893313166883 119 18 -2.2897374490073936 119 19 -2.5910431212414444 119 20 -1.9434900784880647 119 21 -3.2320729730478064 119 22 -1.7146724625244865 119 23 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5 -0.24688546512969367 120 6 -0.6730160950871777 120 7 0.1912369092982118 120 8 0.6492356053534749 120 9 0.11364766167262275 120 10 0.01341728883146942 120 11 -10.734958375672 120 12 -1.890346772330627 120 13 -6.78580729090606 120 14 -7.73836161507252 120 15 -11.195171777382708 120 16 -11.65897622387918 120 17 -13.147945944745832 120 18 -3.5002637647968697 120 19 -4.222622068609866 120 20 -5.575069025856486 120 21 -0.8110203414688613 120 22 -0.08309351515606522 120 23 -0.006535671674193111 120 24 -6.697561397238722 120 25 -0.46383502360433226 120 26 2.135643548088666 120 27 1.7858425020755746 120 28 0.8505298173495746 120 29 2.0753419420946884 120 30 2.060323706218412 120 31 -1.4632171905616431 120 32 -8.30465250773451 120 33 -0.7770012291955766 120 34 -10.138579562717737 120 35 -1.4429790555093156 120 36 1.0869506286313815 120 37 0.0425570364729424 120 38 0.6370931936534703 120 39 2.250124715701972 120 40 0.5444257689879244 120 41 0.37527944965221494 120 42 -5.82452054353028 120 43 -4.410664850345444 120 44 -7.501149711037591 120 45 -13.849499550257388 120 46 -11.846062291628087 120 47 -14.805632627786533 120 48 -13.99558663755643 120 49 -14.76294368438132 120 50 -14.279846254789545 120 51 -13.692943426496761 120 52 -14.017585449446997 120 53 -15.082910065777405 120 54 -15.717656992677274 120 55 -16.08472400923437 120 56 -13.865505679893992 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 72.0 5 23.0 6 27.0 7 30.0 8 63.0 9 86.0 10 73.0 11 88.0 12 63.0 13 58.0 14 82.0 15 119.0 16 138.0 17 171.0 18 270.0 19 311.0 20 408.0 21 489.0 22 600.0 23 780.0 24 856.0 25 954.0 26 938.0 27 1097.0 28 1160.0 29 1139.0 30 1338.0 31 1506.0 32 1810.0 33 2122.0 34 2531.0 35 2936.0 36 3240.0 37 2642.0 38 1295.0 39 329.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 0.0 100.0 7 99.36000536121163 0.34847875619890095 0.2010454362685967 0.09047044632086852 8 99.29299021578876 0.505964347942635 0.09717196086315508 0.10387347540544163 9 97.27211796246648 1.6152815013404827 0.5361930294906166 0.5764075067024129 10 50.4255461734352 31.42675244605281 7.240986462940624 10.906714917571371 11 31.501524645645546 29.953422913245987 17.21006601213015 21.33498642897832 12 35.31363088057901 13.908993432515748 21.74976544699102 29.02761023991422 13 25.184291649912883 14.026269937005765 26.521243801099047 34.2681946119823 14 23.217697335344393 16.346572817161054 26.63314898609016 33.802580861404394 15 23.73666196899537 16.8042413260855 26.96798872558889 32.491107979330245 16 25.925305082472843 17.356175405659112 27.330025479415315 29.388494032452726 17 29.01431109025706 20.3773837852331 22.75362804571505 27.854677078794786 18 28.975939950405472 23.312780644728907 20.94028550365257 26.770993901213053 19 28.62457269253971 23.992894966150548 19.4684630337154 27.914069307594342 20 27.360482654600304 23.063516004692474 22.302664655605835 27.27333668510139 21 27.701638575210268 21.093723821331636 24.74617163153838 26.458465971919715 22 31.247905636351454 20.903424703438105 21.58032303464915 26.26834662556129 23 31.25251306795336 23.797078139659565 21.552070767993566 23.398338024393514 24 28.287092882991555 23.646294062458114 20.76799356654604 27.298619488004288 25 27.59683688513604 23.37488272349551 22.06473663047849 26.963543760889962 26 25.011748909029873 22.8633769721383 24.075193017791204 28.04968110104062 27 25.347722626269398 18.892650065355095 25.894024198143246 29.865603110232264 28 26.454298278003424 22.82904232821993 25.1854586955792 25.53120069819744 29 25.500251382604322 21.55522037874979 27.58505111446288 25.359477124183005 30 22.308620458506503 24.299503954953746 29.410778924788843 23.981096661750907 31 23.586299529885828 24.41571524513096 29.741437206178645 22.256548018804565 32 24.989946380697052 20.020107238605895 30.86461126005362 24.12533512064343 33 24.163506393261066 21.43839983890996 33.2986542269356 21.09943954089338 34 23.4375 22.428245708154506 35.68267167381974 18.45158261802575 35 24.306114239742556 24.396621078037008 34.395950657012605 16.90131402520783 36 22.350137611599653 23.790024837215544 33.26173054977512 20.59810700140968 37 22.51217464315701 24.218303946263646 33.66918555835433 19.60033585222502 38 23.479281999664487 25.4521053514511 34.83308169770173 16.235530951182685 39 21.544100339621373 19.65096338141834 31.910958673795353 26.893977605164938 40 13.723178495637821 15.468049988210328 25.8665409101627 44.94223060598915 41 15.644005946749562 18.113934315448034 27.236788755237196 39.00527098256521 42 18.76573039363737 24.084700828886877 38.088526460619484 19.06104231685627 43 13.387123886367458 14.193982181879308 28.166078332492855 44.25281559926038 44 13.90712866619524 15.829881806243055 26.6895646024851 43.57342492507661 45 19.65124077800134 22.575452716297786 37.90744466800805 19.865861837692826 46 20.487461445621562 22.74373072281078 38.111841223011936 18.65696660855572 47 18.131849716794584 21.614103294567148 35.15433857291283 25.09970841572544 48 20.185023798350873 23.70114634309848 37.101964201917276 19.011865656633372 49 14.355836471221087 16.191500806885422 25.95145239376009 43.5012103281334 50 17.428389133281843 25.793344303032338 38.23163974612982 18.546626817555996 51 20.44973278209136 25.484857651843633 35.01394911095425 19.051460455110753 52 19.730338589825493 26.4147136948993 34.92148885377089 18.93345886150432 53 19.03083996107252 27.336487801604083 36.09517097889191 17.53750125843149 54 18.10373750251627 22.794068308394284 32.24518553311414 26.857008655975307 55 20.271131841213382 24.27435320962384 36.790711721083184 18.663803228079594 56 20.126841381161707 25.13002919365122 34.91158014831717 19.831549276869904 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 1.0 12 2.0 13 5.0 14 7.0 15 17.5 16 28.0 17 41.0 18 54.0 19 53.5 20 53.0 21 80.0 22 122.5 23 138.0 24 172.5 25 207.0 26 228.0 27 249.0 28 317.0 29 490.5 30 596.0 31 713.0 32 830.0 33 946.0 34 1062.0 35 1146.0 36 1230.0 37 1162.5 38 995.0 39 895.0 40 858.0 41 821.0 42 747.5 43 674.0 44 625.5 45 577.0 46 541.0 47 500.0 48 495.0 49 487.0 50 479.0 51 508.5 52 538.0 53 609.0 54 723.5 55 767.0 56 831.0 57 895.0 58 1012.5 59 1130.0 60 1234.5 61 1339.0 62 1547.5 63 1890.0 64 2024.0 65 2060.5 66 2097.0 67 2003.5 68 1910.0 69 1845.5 70 1781.0 71 1582.0 72 1239.0 73 1095.0 74 929.0 75 763.0 76 640.5 77 518.0 78 433.0 79 267.5 80 187.0 81 131.5 82 76.0 83 55.5 84 35.0 85 24.5 86 14.0 87 14.5 88 8.0 89 1.0 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.013403029084573114 10 0.0 11 0.0033507572711432786 12 0.0 13 0.0 14 0.030156815440289503 15 0.1407318053880177 16 0.05361211633829246 17 0.023455300898002948 18 0.006701514542286557 19 0.02010454362685967 20 0.030156815440289503 21 0.0033507572711432786 22 0.006701514542286557 23 0.0 24 0.0 25 0.0 26 0.18094089264173704 27 0.023455300898002948 28 0.17759013537059373 29 0.030156815440289503 30 0.02680605816914623 31 0.21444846535316983 32 0.013403029084573114 33 0.15748559174373408 34 0.06701514542286557 35 0.04020908725371934 36 0.1675378635571639 37 0.23120225170888623 38 0.13067953357458784 39 0.35182951347004426 40 0.5260688915694948 41 0.830987803243533 42 0.15078407720144751 43 0.33172496984318456 44 0.492561318858062 45 0.08041817450743868 46 0.05361211633829246 47 0.023455300898002948 48 0.03350757271143279 49 0.3350757271143278 50 0.21779922262431312 51 0.3116204262163249 52 0.3451279989277577 53 0.15078407720144751 54 0.12732877630344458 55 0.14408256265916097 56 0.14408256265916097 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 29844.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.4771478354108 #Duplication Level Percentage of deduplicated Percentage of total 1 94.64113769350419 85.62860206406648 2 3.5701059180801424 6.460260018764242 3 0.8999333382712391 2.44270205066345 4 0.33701207317976445 1.2196756466961534 5 0.18517146878009036 0.8376893177858197 6 0.08888230501444337 0.48250904704463204 7 0.08147544626323976 0.5160166197560648 8 0.05555144063402711 0.4020908725371934 9 0.044441152507221686 0.3618817852834741 >10 0.08888230501444337 1.2665862484921593 >50 0.007406858751203614 0.38198632891033374 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCGTCCGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC 60 0.2010454362685967 No Hit GCGTCCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG 54 0.18094089264173704 No Hit GCGTCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC 41 0.13738104811687443 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.006701514542286556 0.0 0.0 0.0 0.0 11 0.013403029084573113 0.0 0.0 0.0 0.0 12 0.013403029084573113 0.0 0.0 0.0 0.0 13 0.02010454362685967 0.0 0.0 0.0 0.0 14 0.030156815440289506 0.0 0.0 0.0 0.0 15 0.030156815440289506 0.0 0.0 0.0 0.0 16 0.03350757271143279 0.0 0.0 0.0 0.0 17 0.03350757271143279 0.0 0.0 0.0 0.0 18 0.03350757271143279 0.0 0.0 0.0 0.0 19 0.03350757271143279 0.0 0.0 0.0 0.0 20 0.03350757271143279 0.0 0.0 0.0 0.0 21 0.04020908725371934 0.0 0.0 0.0 0.0 22 0.04355984452486262 0.0 0.0 0.0 0.0 23 0.046910601796005896 0.0 0.0 0.0 0.0 24 0.05361211633829245 0.0 0.0 0.0 0.0 25 0.06366438815172229 0.0 0.0 0.0 0.0 26 0.07371665996515213 0.0 0.0 0.0 0.0 27 0.08041817450743868 0.0 0.0 0.0 0.0 28 0.09382120359201179 0.0 0.0 0.0 0.0 29 0.09717196086315508 0.0 0.0 0.0 0.0 30 0.09717196086315508 0.0 0.0 0.0 0.0 31 0.10387347540544163 0.0 0.0 0.0 0.0 32 0.10387347540544163 0.0 0.0 0.0 0.0 33 0.10387347540544163 0.0 0.0 0.0 0.0 34 0.10387347540544163 0.0 0.0 0.0 0.0 35 0.10387347540544163 0.0 0.0 0.0 0.0 36 0.10387347540544163 0.0 0.0 0.0 0.0 37 0.1072242326765849 0.0 0.0 0.0 0.0 38 0.1072242326765849 0.0 0.0 0.0 0.0 39 0.1072242326765849 0.0 0.0 0.0 0.0 40 0.11057498994772819 0.0 0.0 0.0 0.0 41 0.11057498994772819 0.0 0.0 0.0 0.0 42 0.11057498994772819 0.0 0.0 0.0 0.0 43 0.11057498994772819 0.0 0.0 0.0 0.0 44 0.12062726176115803 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGAACCT 15 0.008030551 50.49574 39 TGGTGGC 15 0.008194421 50.238983 34 CCAGCCC 15 0.008249594 50.153976 43 CCCACAC 15 0.008249594 50.153976 43 CTTCTGT 25 2.0418747E-5 49.984825 28 AGAAGCA 15 0.008360768 49.98482 50 GCAACAT 30 1.0155509E-6 49.98482 23 CTGCAAT 30 1.0155509E-6 49.98482 49 GCCTGCA 30 1.0155509E-6 49.98482 47 CCGTGGT 15 0.008416771 49.900673 13 CCAGTGA 15 0.008416771 49.900673 13 CGTGGTA 15 0.008416771 49.900673 14 TCTCGCT 15 0.008416771 49.900673 20 AGTGACA 15 0.008416771 49.900673 15 GAGGCCC 15 0.008473053 49.816807 9 ATCCAGT 15 0.008473053 49.816807 11 GAAGCAT 30 1.0395288E-6 49.816807 9 CTCTCGC 15 0.008473053 49.816807 19 GTGGGCG 15 0.008473053 49.816807 10 GATCCAG 15 0.008473053 49.816807 10 >>END_MODULE