FastQCFastQC Report
Fri 17 Jun 2016
SRR1526306_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526306_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30441
Sequences flagged as poor quality0
Sequence length56
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA860.282513715055353No Hit
GCCTAGGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC860.282513715055353No Hit
GCCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC840.27594362865871686No Hit
GCCTAGGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCGAACCTGCGTGCAATC590.19381754870076542No Hit
GCCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC470.15439703032094873No Hit
GCCTAGGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGACAACCTGCGTGCAATC330.10840642554449591No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCCT354.7593858E-850.3926840
ACTAAAA150.00809754350.39267739
ACCAACC150.00809754350.39267738
AACTAAA150.00809754350.39267738
CCAACCA203.962726E-450.39267339
GAGCGCT203.9954757E-450.3089736
GATACCC203.9954757E-450.3089735
TACCCAA203.9954757E-450.3089737
AAACTAA150.00815106350.30896837
AAAACTA150.00815106350.30896836
AGCCCTT150.00815106350.30896837
TAGAGCC251.9854733E-550.2255434
AAGGTGC150.00820484550.22553634
AGAATAT150.0082588950.14238444
GCAGCAT150.0082588950.14238442
ATAGAAT150.0082588950.14238442
ACCAACA402.4738256E-950.1423842
CAACACA451.2369128E-1050.1423844
TTGCCTA204.0616203E-450.1423842
CCAACAC355.016227E-850.05950543