##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526306_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 30441 Sequences flagged as poor quality 0 Sequence length 56 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.43211458230676 39.0 38.0 39.0 33.0 40.0 2 36.588975395026445 39.0 36.0 39.0 30.0 40.0 3 37.16530337373936 39.0 38.0 39.0 33.0 40.0 4 37.015341151736145 39.0 38.0 39.0 33.0 40.0 5 35.52869485233731 39.0 35.0 39.0 24.0 40.0 6 36.7281298249072 38.0 36.0 39.0 32.0 40.0 7 36.60497355540225 38.0 36.0 39.0 31.0 40.0 8 36.55008048355836 38.0 36.0 39.0 31.0 40.0 9 36.50954305049111 38.0 36.0 39.0 31.0 40.0 10 36.43760060444795 38.0 36.0 39.0 31.0 40.0 11 34.86169968135081 38.0 33.0 39.0 27.0 40.0 12 32.911533786669295 37.0 30.0 39.0 18.0 40.0 13 32.49794684800105 36.0 30.0 39.0 17.0 40.0 14 32.221806116750436 36.0 29.0 39.0 15.0 40.0 15 32.71170460891561 37.0 30.0 39.0 18.0 40.0 16 32.28228376203147 36.0 29.0 39.0 17.0 40.0 17 32.34496238625538 36.0 30.0 38.0 17.0 40.0 18 32.440064386846686 36.0 30.0 38.0 18.0 40.0 19 33.28898525015604 36.0 31.0 38.0 23.0 40.0 20 33.56890378108472 36.0 31.0 38.0 24.0 40.0 21 34.87352583686476 38.0 34.0 39.0 26.0 40.0 22 34.53825432804442 38.0 33.0 39.0 25.0 40.0 23 34.77829243454551 38.0 33.0 39.0 26.0 40.0 24 34.347590420814036 38.0 33.0 39.0 24.0 40.0 25 34.613810321605726 38.0 33.0 39.0 26.0 40.0 26 35.03041950001643 38.0 35.0 39.0 27.0 40.0 27 34.965736999441546 38.0 35.0 39.0 27.0 40.0 28 34.96281331099504 38.0 35.0 39.0 27.0 40.0 29 34.7665976807595 38.0 35.0 39.0 27.0 40.0 30 34.64974869419533 38.0 35.0 39.0 26.0 40.0 31 34.422719358759565 38.0 34.0 39.0 25.0 40.0 32 33.31388587759929 38.0 33.0 39.0 18.0 40.0 33 34.58385072763707 38.0 35.0 39.0 26.0 40.0 34 34.33638842350777 38.0 34.0 39.0 25.0 40.0 35 34.21303505141093 38.0 34.0 39.0 25.0 40.0 36 31.890476659768076 35.0 31.0 38.0 19.0 39.0 37 31.608817055944286 35.0 31.0 38.0 17.0 39.0 38 30.76955421963799 35.0 30.0 38.0 10.0 39.0 39 28.896553989684964 33.0 26.0 38.0 4.0 39.0 40 24.5873000229953 30.0 10.0 37.0 4.0 39.0 41 20.94435136822049 24.0 8.0 32.0 4.0 36.0 42 25.08665943957163 30.0 19.0 33.0 4.0 37.0 43 21.215958739857427 25.0 7.0 33.0 4.0 36.0 44 22.18642620150455 27.0 8.0 33.0 4.0 36.0 45 24.848001051213824 30.0 17.0 34.0 4.0 37.0 46 25.13816891692126 30.0 11.0 36.0 4.0 38.0 47 24.288820997996122 30.0 5.0 35.0 4.0 38.0 48 24.257711638908052 30.0 6.0 35.0 4.0 38.0 49 21.520317992181596 26.0 4.0 35.0 4.0 38.0 50 23.973194047501725 29.0 4.0 35.0 4.0 38.0 51 25.62589928057554 32.0 4.0 38.0 4.0 39.0 52 25.414013994284026 32.0 4.0 38.0 4.0 39.0 53 25.031864919023686 32.0 4.0 38.0 4.0 39.0 54 24.13353700601163 31.0 4.0 38.0 4.0 39.0 55 24.40208928747413 31.0 4.0 38.0 4.0 39.0 56 23.511579777274072 30.0 4.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.297018988576319 1 2 2.8335878258192437 1 3 1.6359699119830609 1 4 1.9037168263792807 1 5 3.7675460120976325 1 6 1.910947696930208 1 7 2.4192135811910376 1 8 2.6188664566993367 1 9 2.6320712400511894 1 10 2.489762309748315 1 11 -3.924017127994542 1 12 -6.376673468423029 1 13 -21.605082262367013 1 14 -18.93921666036821 1 15 -11.621310211597994 1 16 -19.983181984452933 1 17 -21.348190383929726 1 18 -20.808812267149584 1 19 -6.9601563808226565 1 20 -10.361989353321192 1 21 -6.461658441683433 1 22 -2.543372148349384 1 23 -2.537150702774852 1 24 -3.056293371927996 1 25 -2.9483405999619947 1 26 3.3407863877802058 1 27 3.6110843388374576 1 28 2.8763409071969193 1 29 2.317209760774304 1 30 3.1858630290396093 1 31 2.7566588414115145 1 32 -11.021718950544575 1 33 1.0644113640330417 1 34 1.9211918807181476 1 35 2.555622756388644 1 36 3.939923661340945 1 37 4.492905458262477 1 38 5.921425489273176 1 39 7.800626149192087 1 40 7.686972495363612 1 41 -2.838443741918244 1 42 -7.291782590767022 1 43 -11.4191896867437 1 44 -14.823093140105762 1 45 -10.101001198446516 1 46 -16.28300083580054 1 47 -16.34635490178548 1 48 -13.91381950459708 1 49 -13.233029313792063 1 50 -13.249654316926813 1 51 -14.800657957639082 1 52 -14.869977723794499 1 53 -15.568461095307452 1 54 -17.512204592067256 1 55 -14.893958956790286 1 56 -13.68011370151304 2 1 0.8151468725578681 2 2 2.351715709800793 2 3 0.5034715599725175 2 4 0.8364788567022927 2 5 2.561217732598621 2 6 0.9030373475564417 2 7 1.6248826649088954 2 8 1.6946739715312376 2 9 1.6782149447314794 2 10 1.3651743071115376 2 11 -4.580576126016954 2 12 -12.108380785496198 2 13 -14.308444160850861 2 14 -17.342644478430174 2 15 -9.457170462092392 2 16 -5.624579479508963 2 17 -4.623734220449176 2 18 -11.697408180135742 2 19 -3.608145833690159 2 20 -4.999431673690342 2 21 1.0591062254226955 2 22 1.7862257422241186 2 23 0.7884920131117639 2 24 1.217272877248007 2 25 1.4702487210663477 2 26 1.471966348228463 2 27 1.6150395135243372 2 28 1.2283514543294132 2 29 1.8399520152238722 2 30 1.2722176763698698 2 31 -3.918357638483016 2 32 -12.883287836503705 2 33 -4.300123902941248 2 34 -2.642420512162534 2 35 -3.234093789425465 2 36 3.2286514134833304 2 37 3.6491348583943193 2 38 4.436257394348978 2 39 6.647692727965985 2 40 10.058758915930522 2 41 5.455557576473318 2 42 3.1518561699449066 2 43 -1.7889985199671656 2 44 -8.492176857969968 2 45 1.5726968898857194 2 46 -11.843317249775488 2 47 -11.532248112068938 2 48 -8.624432556673549 2 49 -12.702376709968727 2 50 -8.444117072365179 2 51 -8.208700146169075 2 52 -7.642555179298784 2 53 -8.316648306909299 2 54 -14.538572423313136 2 55 -8.623029490738867 2 56 -7.5568440904385525 3 1 1.2536631327204688 3 2 2.873565303296722 3 3 1.9861951372082913 3 4 1.8603609705234305 3 5 3.861014480566105 3 6 1.9340332825157915 3 7 2.6731550226324785 3 8 2.979226817059697 3 9 3.1258775463575006 3 10 2.0669019493879546 3 11 1.846253142275728 3 12 1.9358265315769714 3 13 -3.5437083884931404 3 14 -3.0535184621700147 3 15 -2.147211112498894 3 16 3.3174936912227437 3 17 3.681651958412612 3 18 2.8955796247423073 3 19 1.3146183939521165 3 20 1.8958935295616897 3 21 4.0845127294877415 3 22 3.710006230028995 3 23 3.592353801729651 3 24 2.5602606821260565 3 25 3.4880332739118813 3 26 1.6228809823748023 3 27 2.5474582127113337 3 28 2.63028234863836 3 29 2.4044845355490736 3 30 2.216831497508082 3 31 2.775239922492595 3 32 2.0430333017076805 3 33 2.619591544213222 3 34 3.019164853691123 3 35 1.856861495127383 3 36 5.441049787467069 3 37 6.115090142947167 3 38 7.094285849633536 3 39 9.108058581624523 3 40 11.643616639507758 3 41 11.6334031049286 3 42 9.547181373196938 3 43 2.7486031060490923 3 44 3.7979654184527973 3 45 6.429404206958889 3 46 -1.2345774123771136 3 47 -3.9392603071908887 3 48 -0.7392699550475328 3 49 -9.627173457936207 3 50 -1.8053975601700571 3 51 -2.6289237234048457 3 52 -2.9555633093800857 3 53 -2.529046680893039 3 54 -8.30330819567086 3 55 -1.8877652630965898 3 56 0.9792331453338043 4 1 1.283268395878359 4 2 2.5566793383844413 4 3 1.5980372424714488 4 4 1.6969837775409715 4 5 3.6044355331976803 4 6 1.5667087211122848 4 7 2.5097778296500195 4 8 2.5932619047789913 4 9 2.6927635112697814 4 10 2.094314230089708 4 11 3.2059022650827487 4 12 2.658414250875218 4 13 4.549493365892829 4 14 0.43442013432121485 4 15 5.477788887501102 4 16 6.324072638591161 4 17 5.573099326833667 4 18 5.749746291408972 4 19 4.183039446583699 4 20 4.292823354123094 4 21 4.165653080364933 4 22 3.975357107221974 4 23 3.5813888894489523 4 24 4.190743138266406 4 25 3.2764104668943332 4 26 3.145907298164275 4 27 3.028817861834135 4 28 3.6862034012699354 4 29 2.530581026777149 4 30 2.549068339613342 4 31 2.714932904948732 4 32 2.493691196444523 4 33 2.5077494389500643 4 34 2.648550818603404 4 35 2.9083965828466773 4 36 5.048505927817946 4 37 4.896888388561202 4 38 6.2466981303352895 4 39 9.07516384478242 4 40 11.497783306174423 4 41 6.569806613700532 4 42 8.531830496003959 4 43 8.33851538675085 4 44 12.033711032487883 4 45 9.778088417485204 4 46 7.543931359552708 4 47 7.61666074544069 4 48 7.688361623899837 4 49 4.9386160157480035 4 50 7.5772778784264325 4 51 4.6791903116828735 4 52 4.263734936233952 4 53 4.477532266475382 4 54 -1.7122994237410367 4 55 3.9927171930437595 4 56 2.3772594611232805 5 1 1.805504217991782 5 2 3.4816079389556407 5 3 2.2255362224796045 5 4 2.598248567422651 5 5 4.739890449558352 5 6 2.66707591817471 5 7 2.829647270694494 5 8 3.5761260418658978 5 9 3.023648864186953 5 10 1.8115724920236147 5 11 4.695090351578052 5 12 6.251233508321157 5 13 5.72615595259213 5 14 6.739601692868746 5 15 6.508796639439087 5 16 6.901796016804141 5 17 6.280973663838974 5 18 6.338893578230678 5 19 5.7526028900761474 5 20 4.598412909406655 5 21 3.6087375356892863 5 22 4.021052741656902 5 23 3.1611522513420596 5 24 3.1140397518935004 5 25 2.661967382439009 5 26 2.4237530753980607 5 27 2.2213923212384614 5 28 3.0077048292585147 5 29 2.7508992642312435 5 30 3.3675097920817194 5 31 2.3493678294693368 5 32 4.52510694511853 5 33 2.787227203127955 5 34 3.4988160164818183 5 35 4.170330487375445 5 36 5.05054591149808 5 37 6.193094018916156 5 38 7.343801353509505 5 39 9.163291139764059 5 40 8.288480980593029 5 41 5.0704186088045695 5 42 11.819468194902367 5 43 15.458874423878548 5 44 14.650194100623342 5 45 11.885799555796101 5 46 14.05515126979343 5 47 12.643588530018416 5 48 13.465187409293552 5 49 12.421276154466895 5 50 11.088497788667155 5 51 9.743653795974996 5 52 10.234359171240072 5 53 10.233550218331768 5 54 10.054734439988056 5 55 9.365626209771627 5 56 9.089213765488843 6 1 1.8189409104982417 6 2 3.022176414407838 6 3 2.1500840260971756 6 4 2.381194303856759 6 5 3.9693478138994394 6 6 1.852088838071353 6 7 2.8606550226324785 6 8 3.157004594837474 6 9 2.6272664352463906 6 10 2.5294019493879603 6 11 4.657364253386838 6 12 6.8677709760214185 6 13 7.091013833729086 6 14 6.947870426718879 6 15 6.988899998612215 6 16 8.092493691222742 6 17 7.549707513968169 6 18 7.149746291408977 6 19 6.1923961717298965 6 20 5.6542268628950225 6 21 4.2650682850432915 6 22 3.9905617855845534 6 23 3.345131579507431 6 24 4.597760682126058 6 25 3.9477554961341 6 26 3.7798254268192437 6 27 3.5071804349335522 6 28 3.517782348638356 6 29 3.7142067577713007 6 30 3.0321092752858547 6 31 4.215517700270375 6 32 5.7860888572632305 6 33 4.555702655324332 6 34 3.916387075913349 6 35 4.583250384016267 6 36 3.5993831208004075 6 37 3.2887012540582745 6 38 4.8817858496335305 6 39 4.78861413718008 6 40 3.6755610839522 6 41 1.4528475493730468 6 42 8.76107026208583 6 43 12.795825328271317 6 44 10.779909862897242 6 45 8.393293095847778 6 46 9.718200365400666 6 47 11.337128581698 6 48 10.746841156063578 6 49 10.46727098650824 6 50 10.307102439829944 6 51 11.18913183215071 6 52 12.122214468397694 6 53 12.268175541329182 6 54 11.373080693218032 6 55 11.288623625792301 6 56 10.61117758977825 7 1 1.8520158458987623 7 2 3.388584683141687 7 3 2.5046059899214654 7 4 2.574992753469168 7 5 4.111983472814167 7 6 2.527541034453776 7 7 3.248251921857282 7 8 3.320312088377527 7 9 3.20969537581486 7 10 2.276688771093383 7 11 3.4160205841361915 7 12 5.553559089716508 7 13 6.191272231661898 7 14 6.041927274264097 7 15 5.880889662694901 7 16 6.925051830757624 7 17 6.211206221978504 7 18 5.176102880556261 7 19 5.33399823891336 7 20 4.365854769871767 7 21 2.8878073031311473 7 22 3.393145764912717 7 23 2.5565010885513573 7 24 3.369853705381871 7 25 2.9875487777878504 7 26 0.9586367963282925 7 27 2.9190667398431174 7 28 2.2402629687933953 7 29 2.541596938649853 7 30 2.181463280453819 7 31 3.1865771317949196 7 32 4.478595317211557 7 33 3.3221109240581868 7 34 2.84765322578415 7 35 1.8680049059800936 7 36 2.0040342835911 7 37 2.0070475072882488 7 38 2.878685074439737 7 39 4.140035325810569 7 40 -1.1998911124302296 7 41 -10.627255809800081 7 42 3.8194681949023668 7 43 7.575153493645992 7 44 7.301356891321014 7 45 4.839287927889121 7 46 7.7528256883980795 7 47 8.015681553274227 7 48 7.302396711619135 7 49 10.607322666094802 7 50 7.69314895145785 7 51 9.232025888998255 7 52 9.908777775891231 7 53 9.861457195075953 7 54 10.333804207429917 7 55 10.505161093492557 7 56 10.29851609107024 8 1 1.2780425653445775 8 2 2.1763135302470786 8 3 1.9959469102579348 8 4 1.9060169988922198 8 5 3.43459604084979 8 6 1.9149729988278494 8 7 3.0592365829161636 8 8 2.901212632662535 8 9 2.9241931491943802 8 10 1.4201820912319292 8 11 2.7505084614246655 8 12 4.4743903613642075 8 13 5.446096576045868 8 14 5.074584374709417 8 15 5.087717965515292 8 16 5.394621350797209 8 17 5.92322997259701 8 18 5.444781752401884 8 19 4.318164493242897 8 20 4.6627375011929 8 21 3.2485198216863225 8 22 4.217984953433252 8 23 2.55600628399916 8 24 3.0828670651047787 8 25 2.3581573873870596 8 26 2.275806514289698 8 27 2.0239653049099147 8 28 1.8567894408369412 8 29 1.8934029752653885 8 30 1.5763173131109198 8 31 2.3483782203649355 8 32 2.3440084790126434 8 33 2.0460099839295367 8 34 1.3835265558187828 8 35 2.535496246900429 8 36 -0.451680708986828 8 37 -0.4961687223010607 8 38 0.48036740991722127 8 39 3.084122411411755 8 40 -2.8426422257404695 8 41 -10.601525973085582 8 42 2.367216834189847 8 43 6.678567645056187 8 44 6.60071364540315 8 45 2.729936121852507 8 46 6.2609899635094095 8 47 6.69504820344741 8 48 6.217290328640413 8 49 8.849776896673724 8 50 4.804974780255474 8 51 6.171164929077424 8 52 6.594082080690839 8 53 6.916875304922566 8 54 7.1749719461731125 8 55 4.30328083619419 8 56 5.663187046043024 9 1 2.2189409104982474 9 2 3.711065303296728 9 3 2.7723062483194028 9 4 2.9367498594123163 9 5 4.880458925010544 9 6 2.6965332825157944 9 7 3.149543911521363 9 8 3.2458934837263627 9 9 3.5161553241352763 9 10 2.929401949387959 9 11 4.657364253386838 9 12 6.645548753799197 9 13 5.224347167062419 9 14 7.058981537829986 9 15 6.544455554167772 9 16 7.6480492467782994 9 17 7.194151958412615 9 18 7.105301846964533 9 19 6.081285060618782 9 20 6.054226862895028 9 21 4.376179396154406 9 22 4.701672896695662 9 23 4.322909357285212 9 24 5.042205126570501 9 25 4.169977718356321 9 26 3.5576032045970223 9 27 3.462735990489108 9 28 3.5400045708605816 9 29 4.025317868882411 9 30 3.8765537197303033 9 31 3.3710732558259267 9 32 5.808311079485456 9 33 3.5334804331021132 9 34 3.3608315203577916 9 35 3.1388059395718244 9 36 2.6660497874670703 9 37 2.4220345873916074 9 38 3.6817858496335347 9 39 5.321947470513411 9 40 -0.0355500271589122 9 41 -2.2804857839602874 9 42 6.6055147065302755 9 43 11.084714217160204 9 44 10.291020974008351 9 45 6.237737540292223 9 46 10.562644809845107 9 47 11.914906359475776 9 48 11.302396711619135 9 49 13.578382097619347 9 50 10.662657995385501 9 51 10.455798498817376 9 52 10.811103357286584 9 53 11.179286652440293 9 54 10.639747359884698 9 55 10.533068070236741 9 56 9.544510923111584 10 1 1.3123752539325864 10 2 1.8262168184482377 10 3 1.2778618038749556 10 4 1.919073091735548 10 5 3.4254084199600427 10 6 1.6707757067582207 10 7 2.595503507480963 10 8 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3.2660497874670718 117 37 3.644256809613829 117 38 5.348452516300199 117 39 7.899725248291187 117 40 4.2977833061744235 117 41 3.608403104928602 117 42 0.4721813731969391 117 43 -5.893063560617573 117 44 -1.864534581547204 117 45 1.1710708736255562 117 46 -3.592910745710448 117 47 -6.418426973857555 117 48 -4.430936621714199 117 49 -5.377173457936207 117 50 -3.62623089350339 117 51 -4.699757056738179 117 52 -4.388896642713419 117 53 -5.68738001422637 117 54 -8.515808195670859 117 55 -5.600265263096592 117 56 -2.49993352133286 118 1 0.789647981205313 118 2 2.462580454811878 118 3 1.3460436220567757 118 4 1.0781640008264617 118 5 3.2663175108691362 118 6 1.0571393431218539 118 7 1.7091398711173227 118 8 1.7368025746354547 118 9 0.6504987584787116 118 10 0.7809171009031104 118 11 -9.062837766815182 118 12 -10.922128013877575 118 13 -9.973632630917383 118 14 -0.8925336136851634 118 15 -17.552514142801925 118 16 -6.034779036049983 118 17 -8.036151071890416 118 18 -0.689647647984966 118 19 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119 2 2.065451268209003 119 3 0.9489144354539008 119 4 1.0829486898216771 119 5 1.4289969367064543 119 6 1.1456560895333396 119 7 1.8431111629833552 119 8 1.9616829574105736 119 9 1.9208336867083773 119 10 1.620630019563393 119 11 -5.285325805092693 119 12 -6.6924629421072375 119 13 0.672300383436685 119 14 -1.4954044270822955 119 15 -6.9257198844287196 119 16 -7.518032624566729 119 17 -6.198830497727737 119 18 -1.1800782699945351 119 19 -0.7467851148198115 119 20 -0.5141941897365534 119 21 0.11302150141756329 119 22 0.36132201950267984 119 23 -0.3835233912528011 119 24 -4.686449844189735 119 25 0.8027262563680182 119 26 2.4616967718484872 119 27 1.4147827741148404 119 28 1.668659541620812 119 29 2.530581026777149 119 30 1.373629743122116 119 31 0.4693188698610129 119 32 -10.208063189520391 119 33 0.6831880354412974 119 34 0.8415332747437532 119 35 1.0136597407414172 119 36 4.188856805010932 119 37 5.054783125403304 119 38 6.158978832089673 119 39 7.91726910794031 119 40 10.866204358806005 119 41 7.885596087384741 119 42 4.216041022319747 119 43 -1.8544670693895036 119 44 -0.24699072189807936 119 45 -1.7482273719884809 119 46 -3.1578230264122027 119 47 -7.804391886138257 119 48 -6.750234867328233 119 49 -5.991208545655505 119 50 -5.650792297012162 119 51 -5.33835354796625 119 52 -4.99942295850289 119 53 -12.803169487910582 119 54 -11.993001178127 119 55 -12.656405613973783 119 56 -10.640284398525843 120 1 0.1911631327204688 120 2 1.936065303296722 120 3 0.9445284705416199 120 4 1.1311943038567591 120 5 2.9026811472327694 120 6 0.9965332825157915 120 7 1.568988355965807 120 8 1.8125601503930255 120 9 1.3967108796908292 120 10 0.8377352827212903 120 11 -10.82041352439094 120 12 -4.460006801756361 120 13 -4.9395417218264726 120 14 -6.241018462170015 120 15 -6.647211112498894 120 16 -9.974172975443924 120 17 -11.047514708254052 120 18 -3.7085870419243605 120 19 -3.18538160604788 120 20 -4.5624398037716425 120 21 -0.9154872705122585 120 22 0.12667289669565918 120 23 -1.5743128649370135 120 24 -6.918905984540611 120 25 -2.0328000594214544 120 26 0.268714315708138 120 27 2.7349582127113337 120 28 2.19278234863836 120 29 2.8419845355490736 120 30 2.070998164174746 120 31 -2.9539267441740726 120 32 -12.269466698292323 120 33 -4.755408455786775 120 34 -11.439168479642209 120 35 -3.622305171539285 120 36 -2.3506168791995954 120 37 -2.6140765237195005 120 38 -4.218214150366467 120 39 -1.3086080850421453 120 40 -5.210550027158909 120 41 -1.6999302284047317 120 42 -4.119485293469726 120 43 -6.20973022728424 120 44 -6.639534581547204 120 45 -10.320595793041111 120 46 -11.276244079043781 120 47 -12.751760307190889 120 48 -8.280936621714199 120 49 -14.502173457936205 120 50 -7.867897560170057 120 51 -9.358090390071514 120 52 -9.038896642713418 120 53 -11.112380014226371 120 54 -12.24080819567086 120 55 -11.575265263096592 120 56 -8.416600187999528 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 205.0 5 23.0 6 35.0 7 39.0 8 87.0 9 93.0 10 55.0 11 52.0 12 70.0 13 50.0 14 84.0 15 130.0 16 166.0 17 244.0 18 276.0 19 358.0 20 425.0 21 520.0 22 667.0 23 790.0 24 904.0 25 980.0 26 943.0 27 1058.0 28 1063.0 29 1043.0 30 1188.0 31 1438.0 32 1771.0 33 2194.0 34 2496.0 35 2933.0 36 3348.0 37 2851.0 38 1466.0 39 396.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 100.0 0.0 5 0.0 100.0 0.0 0.0 6 100.0 0.0 0.0 0.0 7 99.28386058276666 0.6175881212837949 0.08212607995795144 0.01642521599159029 8 98.82395453500213 0.9099569659341021 0.11169146874281397 0.15439703032094873 9 95.23543521834851 3.0986100614464562 0.867479380935169 0.7984753392698715 10 40.85608225748169 38.39886994513978 9.388653460793009 11.356394336585526 11 25.038599257580234 32.807726421602446 19.253638185342137 22.900036135475183 12 35.57373279458625 15.853618475082948 21.930948391971356 26.64170033835945 13 27.41697053316251 15.071778193883251 22.27916297099307 35.23208830196117 14 20.608629925400113 20.076243057609517 25.216076768871797 34.09905024811857 15 23.245945857044177 20.272359461859807 24.3972237755337 32.08447090556232 16 23.98961687586252 20.01380035486627 31.280804363540778 24.715778405730433 17 27.95031055900621 26.671924808570775 19.612869302310294 25.76489533011272 18 31.245072273324574 26.524310118265443 20.607752956636006 21.62286465177398 19 27.13980614424183 30.218498439296866 18.60358140298998 24.038114013471333 20 26.83688222923239 27.438222923238698 19.331624605678233 26.393270241850686 21 25.590564116042973 24.095673029536417 25.955251831652266 24.358511022768344 22 26.58431617333027 25.45418706264989 21.46916784388449 26.492328920135357 23 25.639762162872444 28.93466049078545 24.58197825301403 20.843599093328077 24 23.402647744817845 28.665286948523374 22.170756545448572 25.76130876121021 25 24.483426957064484 28.885384842810684 23.2121152393154 23.419072960809434 26 22.771560236998027 28.22909809084924 27.452271231073073 21.547070441079658 27 23.18840579710145 22.307667028163923 29.42259029215551 25.08133688257912 28 20.668092808951783 26.246503208820144 30.212275793977287 22.87312818825078 29 20.96477392218717 25.391035751840167 31.936777076761302 21.707413249211356 30 18.72104104367257 26.827905754000852 36.31165587723045 18.13939732509612 31 18.001317089232796 27.148501810997693 37.869608165953245 16.980572933816266 32 21.97437582128778 21.208935611038108 37.26018396846255 19.556504599211564 33 19.549119631397073 23.620207339147605 39.40760243541221 17.423070594043114 34 18.243687532877434 23.56325618095739 43.18779589689637 15.005260389268807 35 19.554357828316025 25.601419744971736 41.7707374786381 13.073484948074142 36 17.236045286993154 24.601764086361243 40.89323328067404 17.268957345971565 37 16.28527213460209 25.926047874617236 41.938032991998945 15.850646998781734 38 18.78823761594632 26.202223537925136 42.8688902045918 12.14064864153674 39 16.672708697228355 21.40526645354777 37.51771413505586 24.404310714168012 40 10.754037718400106 17.551276546553492 29.953429996366882 41.74125573867953 41 13.25161504058307 19.181712771243994 32.47639556070896 35.09027662746397 42 13.472235936934268 27.714690102366614 44.30071426220335 14.51235969849577 43 9.23710660734882 15.837864557587741 33.425605536332185 41.49942329873126 44 11.10670761914198 17.1306846329139 31.38148551801579 40.38112222992834 45 14.873396908911543 24.985202236106545 43.78493916474844 16.356461690233477 46 14.84074548861059 24.974525852151334 45.11718108010387 15.067547579134208 47 14.165543981861795 23.40551375151973 41.79344790194855 20.63549436466993 48 14.182439537329127 26.853312302839115 44.029311251314404 14.934936908517349 49 11.56267510465078 18.35261544546623 30.389927156465273 39.69478229341771 50 13.264197530864196 26.9037037037037 46.324279835390946 13.507818930041152 51 16.413974950560316 28.091628213579433 42.27092946605141 13.223467369808834 52 13.681017999604405 29.943297949495616 41.42546317663348 14.950220874266499 53 15.129673512374934 29.377303844128487 42.769220642443386 12.723802001053187 54 12.041970922965595 25.65949608578383 39.00072363660286 23.29780935464772 55 14.588885598657587 26.529134998190372 44.812950350409636 14.069029052742406 56 14.777887462981242 27.137216189536034 41.849292530437644 16.23560381704508 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 2.0 11 4.0 12 12.5 13 61.0 14 101.0 15 138.0 16 175.0 17 152.5 18 130.0 19 162.0 20 194.0 21 234.0 22 304.0 23 334.0 24 370.5 25 407.0 26 540.0 27 673.0 28 851.5 29 1176.0 30 1322.0 31 1445.5 32 1569.0 33 1660.0 34 1751.0 35 1693.0 36 1635.0 37 1489.5 38 1242.5 39 1141.0 40 965.5 41 790.0 42 761.0 43 732.0 44 722.5 45 713.0 46 706.5 47 702.5 48 705.0 49 771.0 50 837.0 51 849.5 52 862.0 53 871.0 54 915.5 55 951.0 56 993.0 57 1035.0 58 1100.5 59 1166.0 60 1180.0 61 1194.0 62 1340.0 63 1416.5 64 1347.0 65 1294.5 66 1242.0 67 1232.5 68 1223.0 69 1050.0 70 877.0 71 737.0 72 511.5 73 426.0 74 341.5 75 257.0 76 201.0 77 145.0 78 125.0 79 69.0 80 33.0 81 25.5 82 18.0 83 13.0 84 8.0 85 6.0 86 4.0 87 3.0 88 1.0 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.026280345586544462 10 0.0 11 0.0 12 0.0 13 0.0 14 0.039420518379816694 15 0.1314017279327223 16 0.022995302388226405 17 0.039420518379816694 18 0.003285043198318058 19 0.019710259189908347 20 0.029565388784862517 21 0.013140172793272231 22 0.006570086396636116 23 0.0 24 0.0 25 0.0 26 0.20038763509740154 27 0.039420518379816694 28 0.18396241910581124 29 0.029565388784862517 30 0.03285043198318058 31 0.23323806708058212 32 0.003285043198318058 33 0.18396241910581124 34 0.08212607995795144 35 0.04270556157813475 36 0.1872474623041293 37 0.22995302388226407 38 0.12811668473440427 39 0.32193423343516964 40 0.5387470845241614 41 0.8409710587694228 42 0.19710259189908347 43 0.31536414703853355 44 0.5321769981275254 45 0.1018363391478598 46 0.05913077756972503 47 0.026280345586544462 48 0.029565388784862517 49 0.3350744062284419 50 0.21681285108899181 51 0.33178936303012385 52 0.35149962222003217 53 0.1872474623041293 54 0.12811668473440427 55 0.15768207351926677 56 0.16753720311422096 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 30441.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.38467855852305 #Duplication Level Percentage of deduplicated Percentage of total 1 95.12611761285163 85.97943562957853 2 3.3873664316348044 6.12332052166486 3 0.7450752344261103 2.020301566965606 4 0.2471469070291488 0.8935317499425117 5 0.12720796685323835 0.5748825597056602 6 0.08722832012793487 0.47304622055780027 7 0.050883186741295335 0.32193423343516964 8 0.05815221341862324 0.4204855293847114 9 0.021807080031983717 0.17739233270917512 >10 0.13447699353056625 1.9808810485857888 >50 0.01453805335465581 1.0347886074701882 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA 86 0.282513715055353 No Hit GCCTAGGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC 86 0.282513715055353 No Hit GCCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC 84 0.27594362865871686 No Hit GCCTAGGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCGAACCTGCGTGCAATC 59 0.19381754870076542 No Hit GCCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 47 0.15439703032094873 No Hit GCCTAGGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGACAACCTGCGTGCAATC 33 0.10840642554449591 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.006570086396636116 0.0 0.0 0.0 0.0 6 0.006570086396636116 0.0 0.0 0.0 0.0 7 0.006570086396636116 0.0 0.0 0.0 0.0 8 0.006570086396636116 0.0 0.0 0.0 0.0 9 0.006570086396636116 0.0 0.0 0.0 0.0 10 0.022995302388226405 0.0 0.0 0.0 0.0 11 0.039420518379816694 0.0 0.0 0.0 0.0 12 0.039420518379816694 0.0 0.0 0.0 0.0 13 0.039420518379816694 0.0 0.0 0.0 0.0 14 0.04599060477645281 0.0 0.0 0.0 0.0 15 0.052560691173088925 0.0 0.0 0.0 0.0 16 0.05584573437140698 0.0 0.0 0.0 0.0 17 0.05913077756972504 0.0 0.0 0.0 0.0 18 0.06570086396636116 0.0 0.0 0.0 0.0 19 0.07555599356131533 0.0 0.0 0.0 0.0 20 0.07884103675963339 0.0 0.0 0.0 0.0 21 0.08212607995795144 0.0 0.0 0.0 0.0 22 0.08869616635458756 0.0 0.0 0.0 0.0 23 0.09855129594954173 0.0 0.0 0.0 0.0 24 0.10512138234617785 0.0 0.0 0.0 0.0 25 0.11497651194113202 0.0 0.0 0.0 0.0 26 0.12811668473440427 0.0 0.0 0.0 0.0 27 0.12811668473440427 0.0 0.0 0.0 0.0 28 0.13468677113104038 0.0 0.0 0.0 0.0 29 0.13797181432935843 0.0 0.0 0.0 0.0 30 0.13797181432935843 0.0 0.0 0.0 0.0 31 0.13797181432935843 0.0 0.0 0.0 0.0 32 0.13797181432935843 0.0 0.0 0.0 0.0 33 0.13797181432935843 0.0 0.0 0.0 0.0 34 0.13797181432935843 0.0 0.0 0.0 0.0 35 0.13797181432935843 0.0 0.0 0.0 0.0 36 0.13797181432935843 0.0 0.0 0.0 0.0 37 0.13797181432935843 0.0 0.0 0.0 0.0 38 0.13797181432935843 0.0 0.0 0.0 0.0 39 0.13797181432935843 0.0 0.0 0.0 0.0 40 0.13797181432935843 0.0 0.0 0.0 0.0 41 0.13797181432935843 0.0 0.0 0.0 0.0 42 0.15111198712263066 0.0 0.0 0.0 0.0 43 0.15768207351926677 0.0 0.0 0.0 0.0 44 0.16096711671758485 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGACCCT 35 4.7593858E-8 50.39268 40 ACTAAAA 15 0.008097543 50.392677 39 ACCAACC 15 0.008097543 50.392677 38 AACTAAA 15 0.008097543 50.392677 38 CCAACCA 20 3.962726E-4 50.392673 39 GAGCGCT 20 3.9954757E-4 50.30897 36 GATACCC 20 3.9954757E-4 50.30897 35 TACCCAA 20 3.9954757E-4 50.30897 37 AAACTAA 15 0.008151063 50.308968 37 AAAACTA 15 0.008151063 50.308968 36 AGCCCTT 15 0.008151063 50.308968 37 TAGAGCC 25 1.9854733E-5 50.22554 34 AAGGTGC 15 0.008204845 50.225536 34 AGAATAT 15 0.00825889 50.142384 44 GCAGCAT 15 0.00825889 50.142384 42 ATAGAAT 15 0.00825889 50.142384 42 ACCAACA 40 2.4738256E-9 50.14238 42 CAACACA 45 1.2369128E-10 50.14238 44 TTGCCTA 20 4.0616203E-4 50.14238 42 CCAACAC 35 5.016227E-8 50.059505 43 >>END_MODULE