FastQCFastQC Report
Fri 17 Jun 2016
SRR1526305_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526305_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38258
Sequences flagged as poor quality0
Sequence length56
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCATAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC1390.36332270374823566No Hit
TTCATAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCGAACCTGCGTGCAATC850.22217575409064771No Hit
TTCATAGGGATGAGATGGACTTAAATGAATTGACGGGTACCCACAGTTCTGAGTCG720.18819593287678393No Hit
TTCATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC690.1803544356735846No Hit
TTCATAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG600.15682994406398662No Hit
TTCATAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG590.15421611166292018No Hit
TTCATAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG590.15421611166292018No Hit
TTCATAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG580.15160227926185374No Hit
TTCATAGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG560.14637461445972086No Hit
TTCATAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGACAACCTGCGTGCAATC560.14637461445972086No Hit
TTCATAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG560.14637461445972086No Hit
TTCATAGGGGCCGCATAGCCTCTCCACCCAAGCGGCCGGAGACCCTGCCTGCAATC480.12546395525118928No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAACC251.9467661E-550.4344438
TTCGTTT251.9467661E-550.4344438
ACCACAA150.00808706350.4344439
AACTAAA150.00808706350.4344438
CTAAAAA150.00808706350.4344440
CCAACCA203.9595983E-450.43443739
ACGCCCA402.3756002E-950.43443739
CCCACAG203.9595983E-450.43443740
TACGCCC402.3756002E-950.43443738
ACCCACA203.9595983E-450.43443739
TACCCAC203.9595983E-450.43443738
TTTCGTT251.9621199E-550.3677337
GTACCCA203.9856124E-450.36772537
GTCCGCC203.9856124E-450.36772537
TACCCAT150.00812956850.36772537
TCACAGT150.00812956850.36772541
ATACAAA150.00812956850.36772536
GGCCGGC354.92455E-850.2348334
CGGGTCC309.885825E-750.2348334
TAGAGCG150.00821507650.2348334