FastQCFastQC Report
Fri 17 Jun 2016
SRR1526304_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526304_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43152
Sequences flagged as poor quality0
Sequence length56
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG1690.39163885799035963No Hit
CTCCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG1360.3151649981460882No Hit
CTCCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG1120.25954764553207266No Hit
CTCCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG910.21088246199480903No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC890.20624768261030776No Hit
CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC890.20624768261030776No Hit
CTCCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG760.1761216166110493No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC730.16916944753429736No Hit
CTCCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAACGATTAAG730.16916944753429736No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC460.10659992584352986No Hit
CTCCATGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT440.10196514645902854No Hit
CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC440.10196514645902854No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAGAA150.007991062550.59787441
AAGCCAT150.00806595150.4788237
TAAGAGA204.017517E-450.30129234
ATATGCC354.8928996E-850.30129234
CAGGGCC150.00817926150.3012934
GTGCCCA150.00817926150.3012935
ACGATTA354.9385562E-850.24238648
CGATTAA354.9385562E-850.24238649
AACGATT402.4974725E-950.18362847
GACCGCT204.0643185E-450.18362846
ATAGAAT204.0643185E-450.18362842
CAGCAAA150.00825545750.18362447
TTCTCAT150.00825545750.18362442
CTTTTAT150.00833217850.0665144
TAGCGCC150.00833217850.0665144
CCTCGCC150.00833217850.0665144
TCTTCCA150.00833217850.0665150
CAGTAGC1350.049.94994427
GTGTCTC150.00840942949.9499425
TGTCTCC150.00840942949.9499426