##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526304_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 43152 Sequences flagged as poor quality 0 Sequence length 56 %GC 54 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.78392658509455 39.0 38.0 40.0 34.0 40.0 2 37.151789024842415 39.0 38.0 40.0 32.0 40.0 3 37.047367445309604 39.0 38.0 40.0 32.0 40.0 4 37.15538097886541 39.0 38.0 40.0 32.0 40.0 5 35.77764645902855 39.0 37.0 40.0 27.0 40.0 6 36.99406748238784 39.0 38.0 40.0 32.0 40.0 7 37.538236929922135 39.0 38.0 40.0 34.0 40.0 8 37.202725250278085 39.0 38.0 40.0 33.0 40.0 9 37.242283092324804 39.0 38.0 40.0 33.0 40.0 10 36.49921208750464 39.0 36.0 39.0 30.0 40.0 11 35.594920281794586 38.0 35.0 39.0 29.0 40.0 12 33.25984890619207 37.0 31.0 39.0 19.0 40.0 13 32.85379588431591 37.0 31.0 39.0 17.0 40.0 14 32.06743604004449 36.0 29.0 39.0 15.0 40.0 15 32.86853448275862 37.0 30.0 39.0 18.0 40.0 16 32.566138301816835 36.0 30.0 39.0 18.0 40.0 17 32.66511401557286 36.0 30.0 39.0 19.0 40.0 18 32.918335187245084 36.0 30.0 39.0 20.0 40.0 19 33.71141546162403 36.0 31.0 39.0 24.0 40.0 20 33.843321282906935 36.0 31.0 39.0 24.0 40.0 21 35.39122172784575 38.0 35.0 39.0 27.0 40.0 22 35.02885150166852 38.0 34.0 39.0 26.0 40.0 23 35.24823878383389 38.0 35.0 39.0 27.0 40.0 24 34.82095847237672 38.0 34.0 39.0 26.0 40.0 25 35.053091397849464 38.0 34.0 39.0 27.0 40.0 26 35.44215795328142 38.0 35.0 39.0 28.0 40.0 27 35.432123655913976 38.0 35.0 39.0 28.0 40.0 28 35.22038375973304 38.0 35.0 39.0 28.0 40.0 29 35.11417779013719 38.0 35.0 39.0 27.0 40.0 30 34.85701705598814 38.0 35.0 39.0 27.0 40.0 31 34.452632554690396 38.0 34.0 39.0 25.0 40.0 32 33.37773451983686 38.0 33.0 39.0 18.0 40.0 33 34.690443084909155 38.0 34.0 39.0 27.0 40.0 34 34.34691323692992 38.0 34.0 39.0 25.0 40.0 35 34.42526418242492 38.0 34.0 39.0 26.0 40.0 36 32.51323229514275 35.0 31.0 38.0 23.0 39.0 37 32.364873007044864 35.0 31.0 38.0 22.0 39.0 38 31.60847701149425 35.0 30.0 38.0 17.0 39.0 39 29.632879124953654 34.0 27.0 38.0 6.0 39.0 40 25.60270671116055 31.0 13.0 37.0 4.0 39.0 41 22.203744901742677 26.0 10.0 33.0 4.0 37.0 42 25.885961253244346 30.0 22.0 34.0 4.0 37.0 43 21.64998146088246 25.0 8.0 33.0 4.0 36.0 44 22.837759547645533 27.0 10.0 33.0 4.0 37.0 45 25.443780126066 30.0 21.0 34.0 4.0 37.0 46 25.243951612903224 30.0 17.0 36.0 4.0 38.0 47 24.627201520207638 30.0 11.0 35.0 4.0 38.0 48 24.615823136818687 30.0 12.0 35.0 4.0 38.0 49 21.97080088987764 26.0 5.0 35.0 4.0 38.0 50 24.3824156470152 29.0 10.0 35.0 4.0 38.0 51 25.96403411197627 32.0 4.0 38.0 4.0 40.0 52 25.762351687059695 32.0 4.0 38.0 4.0 40.0 53 25.39664441972562 32.0 4.0 38.0 4.0 39.0 54 24.474694104560623 31.0 4.0 37.0 4.0 39.0 55 24.691532258064516 31.0 4.0 37.0 4.0 39.0 56 24.103007971820542 31.0 4.0 37.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.2937145193938804 1 2 0.7877109650698273 1 3 1.4816721053967683 1 4 1.2546778542699286 1 5 2.676225215961587 1 6 1.241845376106994 1 7 0.8632178856122437 1 8 1.1826012124578327 1 9 1.0782378459692126 1 10 1.9997087591128633 1 11 -4.311804370776617 1 12 -8.987001213041886 1 13 -22.571821603831193 1 14 -19.24673633174038 1 15 -12.331419047189595 1 16 -21.722402612301288 1 17 -21.879594406279214 1 18 -20.96381211512417 1 19 -6.500615235516342 1 20 -11.532354495725912 1 21 -6.422541422159121 1 22 -3.6564325691938144 1 23 -3.3274325969640657 1 24 -3.7702567240564875 1 25 -3.6782480927181496 1 26 2.0101325541412436 1 27 1.9246778162074776 1 28 1.5780268027342217 1 29 0.9938501987276567 1 30 2.0075707844107384 1 31 1.3941750931023051 1 32 -13.557610058079696 1 33 0.3945198636131195 1 34 1.3966820656681591 1 35 1.3157200083619713 1 36 2.93706596644925 1 37 3.1670156812536483 1 38 3.263258231215186 1 39 5.220092514172244 1 40 8.66167090754946 1 41 -3.1152354024444975 1 42 -9.093795545901871 1 43 -12.873561200408146 1 44 -15.92265013647807 1 45 -12.11404277360387 1 46 -16.61519167518092 1 47 -16.453603697309717 1 48 -13.271049835753262 1 49 -11.212741258185057 1 50 -13.263668346684307 1 51 -14.663647503064665 1 52 -14.170381910358362 1 53 -15.330341140454781 1 54 -18.14562224866468 1 55 -15.777824913429559 1 56 -15.045102826333304 2 1 0.9676023337072266 2 2 1.9018308877003847 2 3 2.079351022224621 2 4 1.985819053496236 2 5 3.456496008998336 2 6 2.091748664308156 2 7 1.4241463188810997 2 8 1.716450342051644 2 9 1.5908103798183433 2 10 2.4430356449929462 2 11 -2.5094832876044677 2 12 -8.346769104724672 2 13 -9.798514060310882 2 14 -14.06607869150827 2 15 -6.119039936938144 2 16 -1.980816538800319 2 17 -2.3198265145964285 2 18 -9.641568401391098 2 19 -1.5307893167542552 2 20 -2.2538245150682705 2 21 2.7987351735855626 2 22 3.297145767363247 2 23 2.7758556041964724 2 24 2.8572094268139168 2 25 2.650572023336011 2 26 3.0387592465977207 2 27 3.4326081837123112 2 28 3.0875045590204877 2 29 3.341625827354349 2 30 3.762309662553868 2 31 0.3976567178605208 2 32 -10.537880851116448 2 33 -0.03604106482014657 2 34 0.4585776169254103 2 35 -0.17441538815639746 2 36 5.508826121188125 2 37 5.353862876611476 2 38 6.346817225412476 2 39 7.6866302317737905 2 40 10.333624485886016 2 41 7.907588582081615 2 42 4.04005358369194 2 43 -3.109537989576424 2 44 -10.086673347309793 2 45 1.6573305339396498 2 46 -10.783470205161578 2 47 -9.419947991313586 2 48 -8.201804187784209 2 49 -11.672315726270163 2 50 -8.503513607806164 2 51 -7.62148115877066 2 52 -7.119704927766485 2 53 -8.438658355154974 2 54 -15.567285691604717 2 55 -8.494265919232268 2 56 -8.7387198476099 3 1 1.3143744022184265 3 2 1.8965607954737749 3 3 2.266439296269411 3 4 2.2269257728638294 3 5 3.51341300504577 3 6 2.4780464245189577 3 7 1.665253038248693 3 8 1.8600603552268709 3 9 1.9373189437182106 3 10 2.1816390705528903 3 11 1.541109597771026 3 12 1.3475655461317153 3 13 -4.322361227636311 3 14 -5.861599113115648 3 15 -2.072399620732611 3 16 2.2908567154816275 3 17 2.2351141968660215 3 18 1.6084974747617373 3 19 0.252082883509253 3 20 0.5419594111504367 3 21 2.308880101121794 3 22 2.691085161302638 3 23 2.0040505976088596 3 24 2.6413991501340774 3 25 2.035288755878831 3 26 1.891723673475191 3 27 2.092423730484377 3 28 1.9810486960428833 3 29 1.8757496745216784 3 30 1.4047339049781158 3 31 1.789488074909272 3 32 0.5472311383433706 3 33 0.6572395675909206 3 34 1.4164168791125036 3 35 0.0927782877329264 3 36 3.777337320134105 3 37 3.522242323251799 3 38 4.171059649654904 3 39 7.09058280094375 3 40 9.899105381801693 3 41 10.054360650592812 3 42 7.815547654838184 3 43 1.51496793927733 3 44 4.326501883256743 3 45 7.07682987517812 3 46 1.736951402216551 3 47 -3.013887385252982 3 48 1.5510284867875974 3 49 -9.002750508878858 3 50 1.7468157729580014 3 51 0.9903765487392207 3 52 0.9153411855404983 3 53 0.2438185881915338 3 54 -3.656877259457154 3 55 0.11548375138696798 3 56 3.8149033407958974 4 1 1.6631797292846215 4 2 1.78512327099277 4 3 2.161660604534198 4 4 2.1934357611129442 4 5 3.314235566737892 4 6 2.047768320327812 4 7 1.7804116751464605 4 8 1.9609220865233823 4 9 2.078525367533331 4 10 2.554337856295156 4 11 2.927126049004869 4 12 3.85716209920653 4 13 4.957505595708774 4 14 3.762176836747262 4 15 6.105038687140482 4 16 5.811812453828676 4 17 5.69516120039129 4 18 5.13337017354748 4 19 4.312208226243293 4 20 4.909566148322391 4 21 3.396032470882858 4 22 3.7406347108521913 4 23 2.4333494616903337 4 24 3.8341136037180945 4 25 3.129687502451489 4 26 2.524508632347107 4 27 2.8525099036140347 4 28 3.1651458366617646 4 29 3.111158996887525 4 30 1.9632924635366749 4 31 3.151219371423174 4 32 4.088163374927781 4 33 3.012116183337099 4 34 3.5519437102915035 4 35 3.7332013194603064 4 36 5.077376489738494 4 37 5.6374009601495665 4 38 6.256645235240487 4 39 8.326187971331528 4 40 11.244189596451125 4 41 5.754517329010362 4 42 9.46830911194747 4 43 8.308152428113992 4 44 11.395636234999788 4 45 9.506716283325396 4 46 8.406947485256111 4 47 9.287914416548823 4 48 8.782716696736674 4 49 6.195251841297406 4 50 9.290098185805629 4 51 7.602597465307962 4 52 8.566535858474303 4 53 7.2357888192922 4 54 1.6108469865279638 4 55 7.864210739244392 4 56 6.385604476714423 5 1 1.587407528512422 5 2 2.1972854331549314 5 3 2.644286087159685 5 4 2.6027021703793523 5 5 3.629872632374962 5 6 2.4599304824899733 5 7 1.880315150049931 5 8 2.352813978415277 5 9 2.1200311590391223 5 10 2.6011525281098287 5 11 3.382724504603324 5 12 6.361023103067534 5 13 6.65538204358522 5 14 7.084570659141082 5 15 7.146544478646273 5 16 7.1438587858750076 5 17 6.897381277611366 5 18 6.1623277025050065 5 19 5.381223670258734 5 20 4.650396264152512 5 21 3.170163745014129 5 22 4.149418494635974 5 23 3.2398166431575106 5 24 3.5614302060347 5 25 3.2431044908684754 5 26 3.1890839219223963 5 27 2.5774133785175053 5 28 2.723543520059451 5 29 3.3669505026790247 5 30 2.9106862859304954 5 31 4.185968406172215 5 32 5.203028239792644 5 33 3.845127766348682 5 34 3.322213980561777 5 35 2.696039157298145 5 36 3.3679170302790347 5 37 4.130161577910183 5 38 4.980583459178714 5 39 7.0525393226828825 5 40 4.209923187184714 5 41 3.1309652054582386 5 42 7.629988648627002 5 43 11.646955516917082 5 44 10.110404574768129 5 45 8.135577287186404 5 46 10.40646485977349 5 47 10.652779281413686 5 48 10.669299708319688 5 49 11.484827130872695 5 50 11.051681197388643 5 51 9.554817542528042 5 52 9.928022344960787 5 53 9.907120865624243 5 54 10.419727295408272 5 55 10.56450031453397 5 56 9.9362801523901 6 1 1.7659789570838527 6 2 2.3401425760120773 6 3 2.751428944302539 6 4 2.763416456093637 6 5 3.737015489517816 6 6 2.5849304824899733 6 7 1.9338865786213617 6 8 2.209956835558131 6 9 2.2450311590391223 6 10 2.690438242395544 6 11 4.007724504603324 6 12 6.82530881735325 6 13 6.708953472156651 6 14 7.3167135162839365 6 15 6.682258764360558 6 16 6.197430214446431 6 17 6.165238420468505 6 18 6.1623277025050065 6 19 5.416937955973019 6 20 5.2218248355810815 6 21 2.8844494592998444 6 22 3.488704208921689 6 23 2.9005309288718024 6 24 4.079287348891839 6 25 3.689533062297045 6 26 2.6533696362081116 6 27 2.73812766423179 6 28 2.0271149486308744 6 29 1.9205219312504553 6 30 2.589257714501926 6 31 2.5966826918864996 6 32 4.953028239792644 6 33 2.7736991949201126 6 34 2.947213980561777 6 35 2.678182014440999 6 36 3.189345601707604 6 37 2.523018720767322 6 38 3.7841548877501374 6 39 5.766825036968598 6 40 1.7813517586132868 6 41 1.8809652054582386 6 42 8.022845791484148 6 43 11.307669802631366 6 44 10.628261717625271 6 45 7.0820058586149734 6 46 11.513607716916344 6 47 11.099207852842255 6 48 11.597871136891118 6 49 12.234827130872691 6 50 10.015966911674358 6 51 10.108388971099465 6 52 9.678022344960787 6 53 10.014263722767105 6 54 11.151870152551126 6 55 9.296643171676823 6 56 7.418423009532958 7 1 1.6305393966442878 7 2 2.38574697161647 7 3 2.546483889357482 7 4 2.4697351374123215 7 5 3.950202302704632 7 6 2.202238174797664 7 7 2.092677787412569 7 8 2.091275516876813 7 9 2.011789400797362 7 10 2.6206580226153235 7 11 2.7156915375703576 7 12 5.443440685485118 7 13 6.435052373255552 7 14 7.431823406393828 7 15 6.784456566558358 7 16 6.570232412248632 7 17 5.828425233655317 7 18 5.638151878329186 7 19 4.14193795597302 7 20 4.474846813603058 7 21 2.860273635124024 7 22 2.4244184946359724 7 23 3.0961353244761938 7 24 3.7243422939467834 7 25 2.2016209743849586 7 26 2.4261718340103116 7 27 2.218622169726295 7 28 1.8133786848946158 7 29 1.6828845686130904 7 30 2.256015956260164 7 31 2.081572801776609 7 32 2.983797470561875 7 33 1.6478750190959346 7 34 2.099137057484853 7 35 2.0787314649904545 7 36 0.494840107202112 7 37 0.872743995492602 7 38 1.745418624013876 7 39 3.777539322682884 7 40 -1.5167251644636366 7 41 -7.183320508827476 7 42 5.722571066209422 7 43 9.731021450983015 7 44 8.165074904438459 7 45 5.577610254219369 7 46 8.34520112350975 7 47 9.006625435259838 7 48 9.041552455572432 7 49 10.81999196603753 7 50 9.27970317541062 7 51 10.574323037033537 7 52 10.13763772957617 7 53 9.432670316173699 7 54 10.966980042661021 7 55 9.842797017830673 7 56 9.738203229313179 8 1 1.8989154650203588 8 2 2.177444163313659 8 3 2.3665083093819064 8 4 2.578892646569834 8 5 4.022729775232101 8 6 2.6781844507439416 8 7 2.0330929278277097 8 8 2.289321914923214 8 9 2.1795549685629254 8 10 3.154723956681252 8 11 4.184311806190621 8 12 6.8134040554484905 8 13 6.90538204358522 8 14 7.973459548029968 8 15 7.483846065947855 8 16 7.302588944605166 8 17 7.018413023643113 8 18 6.392486432663741 8 19 5.160985575020639 8 20 6.144443883200125 8 21 3.2019097767601608 8 22 4.34187881209629 8 23 3.979896008236878 8 24 4.2142079838124715 8 25 3.753025125789108 8 26 2.790274398112878 8 27 2.7480482991524298 8 28 2.8326705041864315 8 29 3.3153632010917278 8 30 3.579337079581286 8 31 3.6542223744261833 8 32 5.222869509633917 8 33 3.484016655237575 8 34 4.203166361514157 8 35 2.9996105858695756 8 36 2.0147424271044336 8 37 2.112304435053037 8 38 2.7266152052104538 8 39 4.431507576651136 8 40 -0.8158704636089347 8 41 -8.710304635811603 8 42 5.124036267674622 8 43 10.333463453425018 8 44 9.251277590641145 8 45 6.470894747503863 8 46 8.630671208979837 8 47 9.303572932207338 8 48 9.0085854226054 8 49 11.635620781666347 8 50 8.968347864055307 8 51 9.64807151078201 8 52 9.854609646548091 8 53 10.988470071973452 8 54 10.594330470011442 8 55 11.082357457391108 8 56 10.362867453977405 9 1 1.3433599094648017 9 2 1.8858819243829998 9 3 2.5402760620970284 9 4 2.303203423512187 9 5 3.534007970720829 9 6 1.990632236875939 9 7 1.3204780573181054 9 8 1.352813978415277 9 9 1.1920862968837298 9 10 2.0344232048015556 9 11 3.138363602347681 9 12 6.236962952691595 9 13 6.233702845590237 9 14 6.931688453627299 9 15 6.683511897192634 9 16 6.685212169333649 9 17 6.212230901671518 9 18 5.394157276439845 9 19 4.629657254218635 9 20 5.29481982304975 9 21 2.9847000858662582 9 22 3.1121377928815903 9 23 2.8278492246111497 9 24 3.223397624581061 9 25 2.5166007314699783 9 26 2.2288708893409463 9 27 2.8750324261365563 9 28 2.3723530438689693 9 29 1.426474312202835 9 30 2.4573654839254786 9 31 2.490479684367699 9 32 3.4442563099680825 9 33 3.4322204981782605 9 34 3.2958981910880922 9 35 3.3078812625613025 9 36 2.8284433460685072 9 37 2.4230813774089306 9 38 3.258778947900513 9 39 2.9494691472442867 9 40 -0.24277104840425778 9 41 -4.232443315845021 9 42 5.181680377950311 9 43 9.797958023182748 9 44 7.493549938176653 9 45 5.8777452069858995 9 46 9.010788168044161 9 47 9.600147702466316 9 48 9.650189432630466 9 49 10.724175501800012 9 50 8.692658640997664 9 51 9.040719798167139 9 52 8.658285502855527 9 53 7.486381517253321 9 54 8.60017842322782 9 55 7.393134399746998 9 56 6.35952576642519 10 1 1.9676023337072266 10 2 2.6472854331549343 10 3 3.0429873858609824 10 4 2.8767281444053268 10 5 3.9837687362710668 10 6 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0.470703803215045 118 34 -0.43585053556725484 118 35 -2.912831810443791 118 36 1.198562191569355 118 37 1.2782030525645602 118 38 1.482887606644148 118 39 2.7259264194570747 118 40 5.632158210226191 118 41 4.190873039559619 118 42 0.5925462523136389 118 43 -2.7459016259400606 118 44 -0.3083742270751877 118 45 -0.722141606822813 118 46 -8.2116226978302 118 47 -9.411736847618572 118 48 -9.839340844675705 118 49 -8.93683185530242 118 50 -6.896475484639009 118 51 -5.974560337656293 118 52 -5.89859055826502 118 53 -7.526634894744417 118 54 -9.262300354361313 118 55 -7.525937473484467 118 56 -6.100010170190545 119 1 0.34335990946480166 119 2 0.8546928405623362 119 3 1.3083072511808496 119 4 1.1979402656174472 119 5 2.0915128440151705 119 6 0.2284490010084923 119 7 -0.030399135664353594 119 8 0.5379991636004604 119 9 0.5975443865523502 119 10 0.5198033217606195 119 11 -9.99029136841255 119 12 -9.451146209101775 119 13 -2.787739649536473 119 14 -3.000085425515003 119 15 -9.875942293840502 119 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119 54 -9.368632492951518 119 55 -11.277430902397251 119 56 -10.457238366128419 120 1 0.5387622083153758 120 2 0.688664743499757 120 3 1.508818106864119 120 4 1.2898942886059572 120 5 2.6138627801582146 120 6 0.7099304824899733 120 7 0.5213250022666784 120 8 1.0079863922083803 120 9 0.8133809127336988 120 10 1.4355121340211596 120 11 -8.511364165347416 120 12 -1.9173020200851738 120 13 -3.3803322421290645 120 14 -5.7331633310067005 120 15 -6.384243698693631 120 16 -9.378308701809726 120 17 -8.742397047511787 120 18 -3.0371796866575558 120 19 -1.66495859575112 120 20 -4.376081568359805 120 21 0.7021834494476309 120 22 0.3347633222221802 120 23 -0.10439517950258193 120 24 -3.4650476264776167 120 25 -0.4089891051906349 120 26 1.5948720992623038 120 27 2.024457713492879 120 28 1.429824308236789 120 29 2.173600748984448 120 30 1.1058833302654705 120 31 -1.6637852884090698 120 32 -10.426282105034943 120 33 -0.8285175538483642 120 34 -10.350199812541671 120 35 -0.08413325649495818 120 36 2.1696411682100667 120 37 2.8265901493387524 120 38 1.2515194197698456 120 39 3.7982289778552953 120 40 -1.662613758628094 120 41 1.4511622497932137 120 42 -3.130479331668564 120 43 -0.6079705914573026 120 44 -2.4641028143944332 120 45 -7.1125754221732045 120 46 -11.802278982591044 120 47 -13.381703477207004 120 48 -12.351020488724648 120 49 -15.25162607109775 120 50 -10.860880378966039 120 51 -11.54185733431925 120 52 -10.515943172280592 120 53 -11.419849577725508 120 54 -12.191110143015374 120 55 -11.410253380047315 120 56 -7.766306054506451 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 278.0 5 14.0 6 54.0 7 42.0 8 99.0 9 128.0 10 88.0 11 60.0 12 78.0 13 79.0 14 137.0 15 157.0 16 198.0 17 267.0 18 345.0 19 440.0 20 531.0 21 668.0 22 862.0 23 986.0 24 1148.0 25 1239.0 26 1338.0 27 1435.0 28 1531.0 29 1543.0 30 1746.0 31 1986.0 32 2437.0 33 2893.0 34 3580.0 35 4102.0 36 4743.0 37 4569.0 38 2671.0 39 680.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 0.0 100.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.4229699666296 0.4263997033741194 0.07183908045977011 0.07879124953652206 8 99.40211345939933 0.2549128661475714 0.21783463107156098 0.12513904338153503 9 98.04844130258431 1.0012747711206396 0.22250550469347546 0.727778421601576 10 56.66944753429737 25.532999629217652 9.346032628846865 8.451520207638117 11 34.934649610678534 27.81331108639229 16.050241008527994 21.201798294401186 12 34.37152391546162 11.417779013718947 22.411475713756023 31.799221357063406 13 25.516777901371896 10.81989247311828 22.545884315906566 41.11744530960326 14 21.543311467049904 13.226397162791775 31.709974270415614 33.520317099742705 15 27.91475135812787 14.37061800622185 25.953011097181594 31.761619538468683 16 33.0049375275273 14.893715663320892 24.15911356313313 27.942233246018684 17 29.631689961291517 20.6615209883411 24.76415641007811 24.94263264028927 18 30.069524913093858 20.567786790266513 27.168018539976824 22.194669756662805 19 34.90032452480297 23.465461288827075 18.662494204914232 22.971719981455728 20 30.77137093969535 26.90640142820709 18.986343928960608 23.335883703136957 21 29.565680912209142 20.88393436543988 26.429961991285804 23.120422731065172 22 27.280943662951035 20.347153020787466 24.685407058932583 27.686496257328912 23 28.25593251761216 22.200593251761216 22.97691879866518 26.566555431961437 24 28.953466814979606 21.405728587319246 24.64543937708565 24.995365220615497 25 30.254924681344146 21.25608342989571 20.342989571263036 28.146002317497103 26 25.32444919090846 20.957444338680844 23.947716667053605 29.77038980335709 27 23.444998261272747 17.95062014605309 24.886982728642636 33.71739886403153 28 22.47864041604755 25.940286032689453 25.19270059435364 26.38837295690936 29 30.094116556168576 21.111317168158006 25.374379897074505 23.420186378598913 30 25.73601001437248 20.455283045111038 31.670452964903333 22.138253975613146 31 26.856067645419067 23.157870284333768 28.393885894815092 21.592176175432076 32 33.17416902322562 16.937091465393355 26.47304251077836 23.415697000602663 33 28.319323828542238 18.766544373751913 28.523661356986953 24.390470440718897 34 24.41536748329621 22.900426874536006 33.11757609502599 19.566629547141797 35 23.92700628376655 23.50499686971039 34.734621003083916 17.833375843439146 36 23.26725778747505 27.59156956501555 28.63608931804466 20.50508332946474 37 24.20257869671274 23.38715297944012 32.44046927633871 19.96979904750842 38 29.814746030272076 24.496239205125825 26.78753830439224 18.901476460209864 39 23.47263889857904 18.621363287518314 24.405218726016884 33.50077908788576 40 15.17786376987272 14.005314933097113 22.980092311995897 47.83672898503426 41 17.10018930098857 16.6865315852205 25.188716726261422 41.02456238752951 42 23.01563334804525 21.631164486979955 31.494343654904878 23.85885851006992 43 12.836973460795944 11.813551043193078 27.01369990463564 48.33577559137534 44 15.080677082120655 13.124869030710842 22.726955225965682 49.067498661202826 45 23.2340109533092 19.985148055323492 33.74176181193725 23.039079179430054 46 21.012106312908763 21.67308316712278 31.119254139802404 26.195556380166057 47 18.409217359050444 21.717359050445104 30.56147997032641 29.311943620178045 48 21.619992581602375 24.142247774480712 33.232103115727 21.005656528189913 49 15.854197177859916 15.112629890508403 22.735197712532255 46.29797521909943 50 24.125995958094265 21.006295152036053 32.62793560826036 22.239773281609327 51 22.056018238496257 23.865909831107803 33.194528451123624 20.88354347927232 52 21.096967863542222 21.981244036953434 33.993437739976265 22.92835035952808 53 21.65815141662796 20.5829075708314 36.16581514166279 21.593125870877845 54 18.708075110832578 20.808671633823085 29.717057772207134 30.7661954831372 55 20.722561471127726 23.50878822354826 32.61974970396341 23.14890060136061 56 23.856087703813817 20.4185441538533 31.423328842848512 24.302039299484367 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 1.0 12 6.0 13 24.0 14 37.0 15 57.0 16 77.0 17 84.5 18 92.0 19 111.5 20 131.0 21 146.0 22 192.5 23 224.0 24 282.0 25 340.0 26 425.5 27 511.0 28 602.5 29 775.0 30 856.0 31 911.0 32 966.0 33 989.0 34 1012.0 35 904.5 36 797.0 37 758.0 38 686.5 39 654.0 40 593.5 41 533.0 42 584.5 43 636.0 44 702.5 45 769.0 46 941.5 47 1398.0 48 1682.0 49 1606.0 50 1530.0 51 1503.0 52 1476.0 53 1596.0 54 1867.5 55 2019.0 56 2217.5 57 2416.0 58 2662.0 59 2908.0 60 3122.5 61 3337.0 62 3456.5 63 3351.5 64 3127.0 65 2860.0 66 2593.0 67 2189.5 68 1786.0 69 1641.0 70 1496.0 71 1299.5 72 952.5 73 802.0 74 682.5 75 563.0 76 463.5 77 364.0 78 264.5 79 125.5 80 86.0 81 65.0 82 44.0 83 32.0 84 20.0 85 13.0 86 6.0 87 4.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.01622172784575454 10 0.0 11 0.0 12 0.0 13 0.0 14 0.025491286614757136 15 0.1807563959955506 16 0.030126065999258437 17 0.020856507230255842 18 0.0046347793845012975 19 0.027808676307007785 20 0.04866518353726362 21 0.009269558769002595 22 0.0023173896922506487 23 0.0 24 0.0 25 0.0046347793845012975 26 0.18307378568780125 27 0.03939562476826103 28 0.18539117538005193 29 0.03244345569150908 30 0.03244345569150908 31 0.2410085279940675 32 0.02317389692250649 33 0.19929551353355582 34 0.11123470522803114 35 0.05793474230626622 36 0.1622172784575454 37 0.24796069707081944 38 0.1761216166110493 39 0.35456062291434925 40 0.5886169818316648 41 0.8412124582869854 42 0.23869113830181682 43 0.36846496106785315 44 0.47043010752688175 45 0.13904338153503892 46 0.07879124953652206 47 0.03707823507601038 48 0.03707823507601038 49 0.31284760845383763 50 0.23869113830181682 51 0.38468668891360774 52 0.4148127549128662 53 0.2131998516870597 54 0.1598998887652948 55 0.19234334445680387 56 0.2271041898405636 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 43152.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.07369299221357 #Duplication Level Percentage of deduplicated Percentage of total 1 93.04295715180736 79.15507971820541 2 4.461877911252758 7.5917686318131254 3 1.2312385933371468 3.1423804226918794 4 0.43856064939663864 1.4923989618094178 5 0.24515812699190978 1.0428253615127918 6 0.1361989594399499 0.6952169076751946 7 0.08989131323036692 0.5353170189098999 8 0.05447958377597995 0.37078235076010385 9 0.049031625398381955 0.3754171301446051 >10 0.2260902726703168 3.494623655913978 >50 0.016343875132793985 1.1378383388950686 >100 0.008171937566396992 0.9663515016685207 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTCCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 169 0.39163885799035963 No Hit CTCCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 136 0.3151649981460882 No Hit CTCCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 112 0.25954764553207266 No Hit CTCCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 91 0.21088246199480903 No Hit CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 89 0.20624768261030776 No Hit CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC 89 0.20624768261030776 No Hit CTCCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG 76 0.1761216166110493 No Hit CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 73 0.16916944753429736 No Hit CTCCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAACGATTAAG 73 0.16916944753429736 No Hit CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 46 0.10659992584352986 No Hit CTCCATGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT 44 0.10196514645902854 No Hit CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 44 0.10196514645902854 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0046347793845012975 0.0 0.0 0.0 0.0 11 0.01853911753800519 0.0 0.0 0.0 0.0 12 0.01853911753800519 0.0 0.0 0.0 0.0 13 0.023173896922506487 0.0 0.0 0.0 0.0 14 0.030126065999258437 0.0 0.0 0.0 0.0 15 0.03476084538375973 0.0 0.0 0.0 0.0 16 0.03476084538375973 0.0 0.0 0.0 0.0 17 0.03707823507601038 0.0 0.0 0.0 0.0 18 0.04171301446051168 0.0 0.0 0.0 0.0 19 0.04171301446051168 0.0 0.0 0.0 0.0 20 0.046347793845012975 0.0 0.0 0.0 0.0 21 0.04866518353726362 0.0 0.0 0.0 0.0 22 0.04866518353726362 0.0 0.0 0.0 0.0 23 0.05561735261401557 0.0 0.0 0.0 0.0 24 0.060252131998516874 0.0 0.0 0.0 0.0 25 0.06256952169076752 0.0 0.0 0.0 0.0 26 0.06488691138301816 0.0 0.0 0.0 0.0 27 0.06952169076751946 0.0 0.0 0.0 0.0 28 0.07415647015202076 0.0 0.0 0.0 0.0 29 0.08342602892102335 0.0 0.0 0.0 0.0 30 0.0903781979977753 0.0 0.0 0.0 0.0 31 0.0903781979977753 0.0 0.0 0.0 0.0 32 0.0903781979977753 0.0 0.0 0.0 0.0 33 0.0903781979977753 0.0 0.0 0.0 0.0 34 0.0903781979977753 0.0 0.0 0.0 0.0 35 0.0903781979977753 0.0 0.0 0.0 0.0 36 0.0903781979977753 0.0 0.0 0.0 0.0 37 0.09269558769002595 0.0 0.0 0.0 0.0 38 0.0950129773822766 0.0 0.0 0.0 0.0 39 0.09733036707452725 0.0 0.0 0.0 0.0 40 0.0996477567667779 0.0 0.0 0.0 0.0 41 0.0996477567667779 0.0 0.0 0.0 0.0 42 0.10428253615127919 0.0 0.0 0.0 0.0 43 0.10428253615127919 0.0 0.0 0.0 0.0 44 0.10659992584352984 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATAGAA 15 0.0079910625 50.597874 41 AAGCCAT 15 0.008065951 50.47882 37 TAAGAGA 20 4.017517E-4 50.301292 34 ATATGCC 35 4.8928996E-8 50.301292 34 CAGGGCC 15 0.008179261 50.30129 34 GTGCCCA 15 0.008179261 50.30129 35 ACGATTA 35 4.9385562E-8 50.242386 48 CGATTAA 35 4.9385562E-8 50.242386 49 AACGATT 40 2.4974725E-9 50.183628 47 GACCGCT 20 4.0643185E-4 50.183628 46 ATAGAAT 20 4.0643185E-4 50.183628 42 CAGCAAA 15 0.008255457 50.183624 47 TTCTCAT 15 0.008255457 50.183624 42 CTTTTAT 15 0.008332178 50.06651 44 TAGCGCC 15 0.008332178 50.06651 44 CCTCGCC 15 0.008332178 50.06651 44 TCTTCCA 15 0.008332178 50.06651 50 CAGTAGC 135 0.0 49.949944 27 GTGTCTC 15 0.008409429 49.94994 25 TGTCTCC 15 0.008409429 49.94994 26 >>END_MODULE