FastQCFastQC Report
Fri 17 Jun 2016
SRR1526303_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526303_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53624
Sequences flagged as poor quality0
Sequence length56
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACTGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC1480.2759958227659257No Hit
TTAACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC720.13426823810234223No Hit
TTAACTGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCGAACCTGCGTGCAATC660.12307921826048038No Hit
TTAACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC650.12121438162017008No Hit
TTAACTGGGCTCGTCTTAATCAGCAACATCTTTTGATACCCAAAAAAAAAAAAAAA570.1062956884976876No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCCG251.9285615E-550.56155439
GCACCGA150.00802463250.5615540
TTCGCCG150.00817608250.3232836
ATATTTT150.00829876450.13427447
CTGCAAT252.0280879E-550.13427449
ACCAACA600.050.08724242
CTCAGGC150.00832964850.08724242
TCCTCCA150.00839167149.99344650
GATATGG204.150557E-449.99344650
TTAAAAG150.00839167149.99344633
GAGGAAT252.085194E-549.920
CTCTCAT204.1891172E-449.912
CGTGGTA204.1891172E-449.914
GAATACC252.085194E-549.923
TTTTGAA150.00845403749.89999831
CCGCAGG150.00845403749.89999828
TCTACCT150.00845403749.89999811
GCGCCAT150.00845403749.89999824
AGGCTAT150.00845403749.89999820
GAATTCC150.00845403749.89999812